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Relevant bibliographies by topics / Picodroplet

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Academic literature on the topic 'Picodroplet'

Author: Grafiati

Published: 1 April 2023

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Journal articles on the topic "Picodroplet"

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Perroud, Thomas D., Robert J. Meagher, Michael P. Kanouff, Ronald F. Renzi, Meiye Wu, Anup K. Singh, and Kamlesh D. Patel. "Isotropically etched radial micropore for cell concentration, immobilization, and picodroplet generation." Lab on a Chip 9, no. 4 (2009): 507. http://dx.doi.org/10.1039/b817285d.

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Wei, F., X. Guo, J. Yin, Y. Shi, and D. Chen. "Performance of picodroplet digital PCR for quantitative detection of HCV RNA." Journal of Clinical Virology 69 (August 2015): 226–27. http://dx.doi.org/10.1016/j.jcv.2015.06.018.

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Josephides, Dimitris, Serena Davoli, William Whitley, Raphael Ruis, Robert Salter, Sinan Gokkaya, Maeva Vallet, et al. "Cyto-Mine: An Integrated, Picodroplet System for High-Throughput Single-Cell Analysis, Sorting, Dispensing, and Monoclonality Assurance." SLAS TECHNOLOGY: Translating Life Sciences Innovation 25, no. 2 (January 15, 2020): 177–89. http://dx.doi.org/10.1177/2472630319892571.

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The primary goal of bioprocess cell line development is to obtain high product yields from robustly growing and well-defined clonal cell lines in timelines measured in weeks rather than months. Likewise, high-throughput screening of B cells and hybridomas is required for most cell line engineering workflows. A substantial bottleneck in these processes is detecting and isolating rare clonal cells with the required characteristics. Traditionally, this was achieved by the resource-intensive method of limiting dilution cloning, and more recently aided by semiautomated technologies such as cell sorting (e.g., fluorescence-activated cell sorting) and colony picking. In this paper we report on our novel Cyto-Mine Single Cell Analysis and Monoclonality Assurance System, which overcomes the limitations of current technologies by screening hundreds of thousands of individual cells for secreted target proteins, and then isolating and dispensing the highest producers into microtiter plate wells (MTP). The Cyto-Mine system performs this workflow using a fully integrated, microfluidic Cyto-Cartridge. Critically, all reagents and Cyto-Cartridges used are animal component-free (ACF) and sterile, thus allowing fast, robust, and safe isolation of desired cells.

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Mosbach, Marcus, Heiko Zimmermann, Thomas Laurell, Johan Nilsson, Elisabeth Csöregi, and Wolfgang Schuhmann. "Picodroplet-deposition of enzymes on functionalized self-assembled monolayers as a basis for miniaturized multi-sensor structures." Biosensors and Bioelectronics 16, no. 9-12 (December 2001): 827–37. http://dx.doi.org/10.1016/s0956-5663(01)00205-6.

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Taly, Valerie, Deniz Pekin, Leonor Benhaim, Steve K. Kotsopoulos, Delphine Le Corre, Xinyu Li, Ivan Atochin, et al. "Multiplex Picodroplet Digital PCR to Detect KRAS Mutations in Circulating DNA from the Plasma of Colorectal Cancer Patients." Clinical Chemistry 59, no. 12 (December 1, 2013): 1722–31. http://dx.doi.org/10.1373/clinchem.2013.206359.

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BACKGROUND Multiplex digital PCR (dPCR) enables noninvasive and sensitive detection of circulating tumor DNA with performance unachievable by current molecular-detection approaches. Furthermore, picodroplet dPCR facilitates simultaneous screening for multiple mutations from the same sample. METHODS We investigated the utility of multiplex dPCR to screen for the 7 most common mutations in codons 12 and 13 of the KRAS (Kirsten rat sarcoma viral oncogene homolog) oncogene from plasma samples of patients with metastatic colorectal cancer. Fifty plasma samples were tested from patients for whom the primary tumor biopsy tissue DNA had been characterized by quantitative PCR. RESULTS Tumor characterization revealed that 19 patient tumors had KRAS mutations. Multiplex dPCR analysis of the plasma DNA prepared from these samples identified 14 samples that matched the mutation identified in the tumor, 1 sample contained a different KRAS mutation, and 4 samples had no detectable mutation. Among the tumor samples that were wild type for KRAS, 2 KRAS mutations were identified in the corresponding plasma samples. Duplex dPCR (i.e., wild-type and single-mutation assay) was also used to analyze plasma samples from patients with KRAS-mutated tumors and 5 samples expected to contain the BRAF (v-raf murine sarcoma viral oncogene homolog B) V600E mutation. The results for the duplex analysis matched those for the multiplex analysis for KRAS-mutated samples and, owing to its higher sensitivity, enabled detection of 2 additional samples with low levels of KRAS-mutated DNA. All 5 samples with BRAF mutations were detected. CONCLUSIONS This work demonstrates the clinical utility of multiplex dPCR to screen for multiple mutations simultaneously with a sensitivity sufficient to detect mutations in circulating DNA obtained by noninvasive blood collection.

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Laurent-Puig, Pierre, Deniz Pekin, Corinne Normand, Steve K. Kotsopoulos, Philippe Nizard, Karla Perez-Toralla, Rachel Rowell, et al. "Clinical Relevance of KRAS-Mutated Subclones Detected with Picodroplet Digital PCR in Advanced Colorectal Cancer Treated with Anti-EGFR Therapy." Clinical Cancer Research 21, no. 5 (September 23, 2014): 1087–97. http://dx.doi.org/10.1158/1078-0432.ccr-14-0983.

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Borsu, Laetitia, Julie Intrieri, Linta Thampi, Helena Yu, Gregory Riely, Khedoudja Nafa, Raghu Chandramohan, Marc Ladanyi, and Maria E. Arcila. "Clinical Application of Picodroplet Digital PCR Technology for Rapid Detection of EGFR T790M in Next-Generation Sequencing Libraries and DNA from Limited Tumor Samples." Journal of Molecular Diagnostics 18, no. 6 (November 2016): 903–11. http://dx.doi.org/10.1016/j.jmoldx.2016.07.004.

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Watanabe, Masaru, Tomoya Kawaguchi, Shun-ichi Isa, Masahiko Ando, Akihiro Tamiya, Akihito Kubo, Hideo Saka, et al. "Multiplex Ultrasensitive Genotyping of Patients with Non-Small Cell Lung Cancer for Epidermal Growth Factor Receptor (EGFR) Mutations by Means of Picodroplet Digital PCR." EBioMedicine 21 (July 2017): 86–93. http://dx.doi.org/10.1016/j.ebiom.2017.06.003.

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Liu, X., R. E. Painter, K. Enesa, D. Holmes, G. Whyte, C. G. Garlisi, F. J. Monsma, M. Rehak, F. F. Craig, and C. A. Smith. "High-throughput screening of antibiotic-resistant bacteria in picodroplets." Lab on a Chip 16, no. 9 (2016): 1636–43. http://dx.doi.org/10.1039/c6lc00180g.

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Pybus, Leon P., Devika Kalsi, Joe T. Matthews, Ellie Hawke, Nicholas Barber, Rachel Richer, Alison Young, and Fay L. Saunders. "Coupling picodroplet microfluidics with plate imaging for the rapid creation of biomanufacturing suitable cell lines with high probability and improved multi‐step assurance of monoclonality." Biotechnology Journal 17, no. 1 (October 25, 2021): 2100357. http://dx.doi.org/10.1002/biot.202100357.

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Conference papers on the topic "Picodroplet"

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Li, X., M. J. Brown, C. A. Smith, G. Cooper, X. Liu, A. Dossang, V. Pawate, A. Bridges, D. Holmes, and B. Leavens. "Picodroplet Mass Spectrometry for Miniaturized High Throughput Analysis of Synthetic Biology Microbial Clones." In IET/SynbiCITE Engineering Biology Conference. Institution of Engineering and Technology, 2016. http://dx.doi.org/10.1049/cp.2016.1249.

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Akamatsu, Hiroaki, Yasuhiro Koh, Masaru Watanabe, Takashi Kikuchi, Masanori Nakanishi, Kazuto Matsunaga, and Nobuyuki Yamamoto. "Abstract 4908: Establishment of multiplexed ultra-sensitive detection of epidermal growth factor receptor mutations using picodroplet digital PCR." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-4908.

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Koh, Yasuhiro, Tomoya Kawaguchi, Masaru Watanabe, Shun-ichi Isa, Masahiko Ando, Akihiro Tamiya, Akihito Kubo, et al. "Abstract 617: Ultrasensitive detection of the pretreatment EGFR T790M mutation in non-small cell lung cancer patients with an EGFR-activating mutation using picodroplet digital PCR." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-617.

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Koh, Yasuhiro, Tomoya Kawaguchi, Masaru Watanabe, Shun-ichi Isa, Masahiko Ando, Akihiro Tamiya, Akihito Kubo, et al. "Abstract 1376: Ultra-sensitive picodroplet digital PCR assay for multiplex genotyping of mutations in epidermal growth factor receptor (EGFR) in non-small cell lung cancer patients." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-1376.

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Samuels, Michael L., Steve K. Kotsopoulos, Frances Long, Holly Gettler, Omo Clement, and Jeff Olson. "Abstract 4894: Targeted sequencing of myeloid cancers using single-molecule enrichment in picodroplets." In Proceedings: AACR 106th Annual Meeting 2015; April 18-22, 2015; Philadelphia, PA. American Association for Cancer Research, 2015. http://dx.doi.org/10.1158/1538-7445.am2015-4894.

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