Academic literature on the topic 'Phytocomplex'
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Journal articles on the topic "Phytocomplex"
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Landina, L. N. "Standardization of Dry Extract of Pumpkin Pulp Juice, Which Has a Hypolipidemic Effect on the Content of Β-Carotene." Medicina 9, no. 1 (2021): 79–92. http://dx.doi.org/10.29234/2308-9113-2021-9-1-79-92.
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The objective is to standardize the content of β-carotene – one of the principal active substances with sufficient hypolipidemic activity in the resultant original product. Search for natural biologically active substances having a hypolipidemic effect is one of the topical questions in modern pharmacy. Most of the research is carried out by means of isolating individual substances from the plant object. We propose to study a native phytocomplex obtained from pumpkin pulp – a dry extract of pumpkin pulp juice. The following materials and methods were used: laboratory-obtained dry extract of pumpkin pulp juice and methods used to determine the qualitative and quantitative content of β-carotene in an individual substance. It was possible to adapt the methods applied to the analysis of an individual substance in relation to the resulting phytocomplex containing both lipophilic and hydrophilic fractions. The rapid method of quantitative determination of β-carotene described in the scientific literature was corrected. An effective alternative method for spectrophotometric determination of β-carotene in the phytocomplex has been developed. Phytocomplexes standardized for the main active substances can be used to obtain effective drugs and biologically active additives with minimal side effects. In addition, the expansion of the search for alternative natural objects containing complexes haaving a hypolipidemic effect is currently relevant. Based on the literature data, in the future it is planned to conduct a pharmacological study to establish the anti-inflammatory effect of the resulting complex on the state of the vascular wall.
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Babaskina, Liudmila Ivanovna, Tatiana Mikhailovna Litvinova, Dmitrii Vladimirovich Babaskin, and Olga Valerevna Krylova. "Control of the Transdermal Delivery Process of Active Substances of the Phytocomplex during Phonophoresis in Model Experiments." Open Access Macedonian Journal of Medical Sciences 7, no. 13 (August 4, 2019): 2079–83. http://dx.doi.org/10.3889/oamjms.2019.607.
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BACKGROUND: The scientific substantiation for the selection of therapeutically significant dosage of phytocomplex in the dosage form for phonophoresis, control over the delivery of active substances into the body, and what affects this process require the study of the kinetics of phytocomplex flavonoids delivery during phonophoresis.
AIM: The aim was to study the possibilities of controlling the process of transdermal delivery of phytocomplex active substances (flavonoids) during phonophoresis in vitro model experiments.
METHODS: Working compositions with different concentrations of phytocomplex for phonophoresis were used. The content of flavonoids in the compositions was determined using the spectrophotometric method and was calculated equivalent to quercetin, the flavonoid prevailing in the phytocomplex. The study of the kinetics of flavonoids delivery from working compositions was carried out using Franz diffusion cells and Carbosyl-P membranes. The authors determined the main parameters of the process and established the dependence of the delivery rate of flavonoids on their initial concentration in the working composition. The authors studied the effect of dimethyl sulfoxide and the base-forming substances of the working composition on the kinetics of phytocomplex flavonoid delivery during phonophoresis.
RESULTS: The authors recorded an increase in the rate of delivery of the active substances from working compositions containing dimethyl sulfoxide into the model medium by almost 1.5-2 times during the first ten minutes of the experiment (approximate duration of the phonophoresis procedure). The authors proposed technological techniques for improvement of the phonophoresis method for the phytocomplex. The possibilities of control over the process of transdermal delivery of the phytocomplex active ingredients during phonophoresis in vitro model experiments were shown.
CONCLUSION: The obtained results provide information for further pharmacological studies of the nature and mechanism of the effect of phytocomplex flavonoids during phonophoresis in the rehabilitation of patients with osteoarthrosis.
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Babaskina, Liudmila Ivanovna, Tatiana Mikhailovna Litvinova, Dmitrii Vladimirovich Babaskin, Mikhail Valentinovich Kiselevsky, Olga Vladimirovna Savinova, and Elizaveta Aleksandrovna Winter. "Influence of Flavonoids on the Cytotoxic Activity of Mononuclear Blood Cells in Model Tests." Open Access Macedonian Journal of Medical Sciences 7, no. 12 (July 2, 2019): 1900–1904. http://dx.doi.org/10.3889/oamjms.2019.331.
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BACKGROUND: The spread of phytocomplex application and justification of its selective effects on tumour cells (mainly due to the presence of flavonoids) require research of its cytotoxic and immunomodulatory activity.
AIM: The goal was to study the direct cytotoxic effect of the phytocomplex and its modulating effect on the cytotoxic activity of the donor's mononuclear blood cells in in vitro experiments.
METHODS: The phytocomplex was a dry extract from marsh cinquefoil, creeping alfalfa and common hop; its main active ingredients were flavonoids. Transplantable monolayer cultures of lung adenocarcinoma, colorectal cancer, erythroblastic leukaemia, and fibroblasts were used as target cells. The cytotoxic activity was assessed using a cytotoxic test based on the selective ability to live cells to reduce MTT (3-[4, 5-dimethyltriazol-2-yl]-2, 5 diphenyltetrazolium bromide) to formazan in mitochondria. Quantitative determination of formazan was performed using spectrophotometry.
RESULTS: A direct cytotoxic effect of the phytocomplex in concentrations of at least 2.5 mg/ml on tumour cells has been established. Its modulating effect on the cytotoxic activity of mononuclear blood cells at a concentration of 0.05 mg/ml was shown. The phytocomplex in doses of 0.25 and 0.5 mg/ml increased the killer activity of the mononuclear cells in a diseased person's blood, but did not affect these blood cells in a healthy donor. Incubation of lymphocytes with a phytocomplex for 24 hours increased the cytotoxic activity of mononuclear cells by 20-25%.
CONCLUSION: The direct cytotoxic effect of the phytocomplex and its modulating effect on the cytotoxic activity of mononuclear blood cells in model experiments in vitro have been established.
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Busato, Isabella, Oriana Bertaiola, Silvio Tundo, Chiara Guarnerio, Marco Lucchetta, Luca Sella, Giovanna Pressi, and Francesco Favaron. "A Phytocomplex Obtained from Salvia officinalis by Cell Culture Technology Effectively Controls the Grapevine Downy Mildew Pathogen Plasmopara viticola." Plants 11, no. 20 (October 11, 2022): 2675. http://dx.doi.org/10.3390/plants11202675.
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The negative impact of using conventional fungicides in plant disease protection has increased the interest in safer alternatives such as plant secondary metabolites, generally having a better toxicological profile. However, cultivation conditions and plant material strongly affect the quality and quantity of secondary metabolites obtained from field grown plants, limiting the standardization needed for industrial production. Plant cell culture technology can provide highly homogeneous biomasses with specific chemical characteristics. A phytocomplex with high rosmarinic acid content (10.12% w/w) was obtained from a selected cell line of Salvia officinalis and was tested against the grapevine downy mildew pathogen, Plasmopara viticola. Grapevine leaf discs were sprayed with the phytocomplex at 5 g/L and then inoculated with P. viticola sporangia. Sporulation level on each disc was assessed after 7 days with an image processing software. The phytocomplex reduced by 95% the sporulation level compared to the control and was also more effective than rosmarinic acid alone, used at the same concentration found in the phytocomplex. Persistence of the phytocomplex was also assessed: when applied 5 days before inoculation, it reduced by 90% the sporulation level compared to the control. These results highlight the possibility to take advantage of cell culture techniques to produce safer pesticides with high quality standards.
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Buriani, Alessandro, Stefano Fortinguerra, Vincenzo Sorrenti, Giada Caudullo, and Maria Carrara. "Essential Oil Phytocomplex Activity, a Review with a Focus on Multivariate Analysis for a Network Pharmacology-Informed Phytogenomic Approach." Molecules 25, no. 8 (April 16, 2020): 1833. http://dx.doi.org/10.3390/molecules25081833.
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Thanks to omic disciplines and a systems biology approach, the study of essential oils and phytocomplexes has been lately rolling on a faster track. While metabolomic fingerprinting can provide an effective strategy to characterize essential oil contents, network pharmacology is revealing itself as an adequate, holistic platform to study the collective effects of herbal products and their multi-component and multi-target mediated mechanisms. Multivariate analysis can be applied to analyze the effects of essential oils, possibly overcoming the reductionist limits of bioactivity-guided fractionation and purification of single components. Thanks to the fast evolution of bioinformatics and database availability, disease-target networks relevant to a growing number of phytocomplexes are being developed. With the same potential actionability of pharmacogenomic data, phytogenomics could be performed based on relevant disease-target networks to inform and personalize phytocomplex therapeutic application.
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Babaskin, Dmitrii Vladimirovich, Tatiana Mikhailovna Litvinova, and Liudmila Ivanovna Babaskina. "The Effect of the Phytocomplex Electrophoresis on the Clinical Symptomatology and Quality of Life of Patients with the Knee Joint Osteoarthritis." Open Access Macedonian Journal of Medical Sciences 7, no. 14 (July 13, 2019): 2236–41. http://dx.doi.org/10.3889/oamjms.2019.603.
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BACKGROUND: Improving the effectiveness of rehabilitation of patients with osteoarthritis necessitates the use of drug electrophoresis with sinusoidal modulated currents (SMC-electrophoresis) in conjunction with drug therapy. The phytocomplex is proposed for electrophoresis composed of the compared, alfalfa and hops dry extract, containing flavonoids, coumestans, polysaccharides, steroids, essential amino acids, vitamins, mineral components and causing its possible use in osteoarthritis.
AIM: The research aims to study the effect of the phytocomplex SMC-electrophoresis on the clinical symptoms and quality of life of patients with the knee joint osteoarthritis.
METHODS: One hundred and eight patients were randomly distributed into 3 groups (n = 36). The phytocomplex SMC-electrophoresis was assigned to the first group, the amplipulse therapy (SMC) – to the second group, and the "basic" drug therapy – to the third group. The drug therapy of the patients of the third group was comparable with the drug treatment of those in the first two groups. The concentration of phytocomplex in the working solution was 10%. The electrotherapy was carried out in the aligned SMC mode in the first and fourth kind of works. Comparative indicators were as follows: WOMAC index, pain level on a visual analogue scale (VAS), Lequesne index, joint range of motion (JROM), articular and tendon indices, quality of life as per Health Assessment Questionnaire (HAQ).
RESULTS: The use of the phytocomplex SMC-electrophoresis had a more pronounced positive effect on pain, knee joint function and quality of life of the patients compared with the treatment with drugs alone or using amplitude therapy. This was especially pronounced immediately after the rehabilitation. The analgesic effect was consistently maintained in the patients of the first group for up to 6 – 12 months, the second group – up to 3 – 6 months in terms of the level of pain according to the WOMAC and Lequesne indices, VAS, articular and tendon indices. Stable results have been obtained for improving the functions of the knee joint for up to 6 – 12 months using the phytocomplex SMC-electrophoresis as per the WOMAC and Lequesne indices. In the treatment by the phytocomplex SMC-electrophoresis, no side effects were registered.
CONCLUSION: The obtained results give grounds for further research on the evaluation of the effectiveness of using the phytocomplex SMC-electrophoresis in microcirculatory disorders in the affected joint, for correcting connective tissue metabolism and electrolyte metabolism in the patients with the knee joint osteoarthritis.
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Arru, Laura, Francesca Mussi, Luca Forti, and Annamaria Buschini. "Biological Effect of Different Spinach Extracts in Comparison with the Individual Components of the Phytocomplex." Foods 10, no. 2 (February 9, 2021): 382. http://dx.doi.org/10.3390/foods10020382.
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The Mediterranean-style diet is rich in fruit and vegetables and has a great impact on the prevention of major chronic diseases, such as cardiovascular diseases and cancer. In this work we investigated the ability of spinach extracts obtained by different extraction methods and of the single main components of the phytocomplex, alone or mixed, to modulate proliferation, antioxidant defense, and genotoxicity of HT29 human colorectal cells. Spinach extracts show dose-dependent activity, increasing the level of intracellular endogenous reactive oxygen species (ROS) when tested at higher doses. In the presence of oxidative stress, the activity is related to the oxidizing agent involved (H2O2 or menadione) and by the extraction method. The single components of the phytocomplex, alone or mixed, do not alter the intracellular endogenous level of ROS but again, in the presence of an oxidative insult, the modulation of antioxidant defense depends on the oxidizing agent used. The application of the phytocomplex extracts seem to be more effective than the application of the single phytocomplex components.
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Moroșan, Elena, Ana Andreea Secăreanu, Adina Magdalena Musuc, Magdalena Mititelu, Ana Corina Ioniță, Emma Adriana Ozon, Adriana Maria Dărăban, and Oana Karampelas. "Advances on the Antioxidant Activity of a Phytocomplex Product Containing Berry Extracts from Romanian Spontaneous Flora." Processes 10, no. 4 (March 25, 2022): 646. http://dx.doi.org/10.3390/pr10040646.
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The present study aimed to evaluate the antioxidant properties of a phytocomplex product obtained using 10% hydroalcoholic extractive solutions, in equal proportions, from Ribes nigrum, Rubus idaeus, Rubus fruticosus and Fragaria moschata fresh fruits harvested from the spontaneous flora of Romania. These plant products were recognized for their rich antioxidant content. The phytochemical profile was assessed using HPLC chromatography and UV-Vis spectrometry. The obtained results highlighted the presence of complex bioactive compounds with antioxidant actions, namely anthocyanins, proanthocyanins and vitamin C. The antioxidant actions of the hydroalcoholic extractive solutions and the phytocomplex product were evaluated using chemiluminescence, electrochemical and superoxide dismutase (SOD) methods. The experimental results showed evident antioxidant activity in both the hydroalcoholic extracts and the phytocomplex product.
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Pressi, Giovanna, Oriana Bertaiola, Chiara Guarnerio, Elisa Barbieri, Flavia Guzzo, Caroline Durand, Laurent Peno-Mazzarino, Veronica Cocetta, Isabella Giacomini, and Alessandra Semenzato. "In Vitro Cultured Melissa officinalis Cells as Effective Ingredient to Protect Skin against Oxidative Stress, Blue Light, and Infrared Irradiations Damages." Cosmetics 8, no. 1 (March 15, 2021): 23. http://dx.doi.org/10.3390/cosmetics8010023.
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Skin is being increasingly exposed to artificial blue light due to the extensive use of electronic devices, which can induce cell oxidative stress, causing signs of early photo aging. The Melissa officinalis phytocomplex is a new standardized cosmetic ingredient obtained by an in vitro plant cell culture with a high content of rosmarinic acid. In this study, we examine the activity of the Melissa officinalis phytocomplex to protect skin against blue light and infrared damages, evaluating the ROS (Radical Oxygen Species) level in keratinocyte cell line from human skin (HaCaT) and Nrf2 (Nuclear factor erythroid 2-related factor 2), elastin, and MMP1 (Matrix Metalloproteinase 1) immunostaining in living human skin explants ex vivo. This phytocomplex demonstrates antioxidant activity by reducing ROS production and thus the oxidant damage of the skin caused by UV and blue light exposure. In addition, it inhibits blue light-induced Nrf2 transcriptional activity, IR-induced elastin alteration, and IR-induced MMP-1 release. This Melissa officinalis phytocomplex is a new innovative active ingredient for cosmetic products that is able to protect skin against light and screen exposure damages and oxidative stress.
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Pressi, Giovanna, Giovanna Rigillo, Paolo Governa, Vittoria Borgonetti, Giulia Baini, Raffaella Rizzi, Chiara Guarnerio, et al. "A Novel Perilla frutescens (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo." Pharmaceutics 15, no. 1 (January 10, 2023): 240. http://dx.doi.org/10.3390/pharmaceutics15010240.
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In the last years, the medicinal plant Perilla frutescens (L.) Britton has gained scientific interest because leaf extracts, due to the presence of rosmarinic acid and other polyphenols, have shown anti-allergic and skin protective potential in pre-clinical studies. Nevertheless, the lack of standardized extracts has limited clinical applications to date. In this work, for the first time, a standardized phytocomplex of P. frutescens, enriched in rosmarinic acid and total polyphenols, was produced through innovative in vitro cell culture biotechnology and tested. The activity of perilla was evaluated in an in vitro inflammatory model of human keratinocytes (HaCaT) by monitoring tight junctions, filaggrin, and loricrin protein levels, the release of pro-inflammatory cytokines and JNK MAPK signaling. In a practical health care application, the perilla biotechnological phytocomplex was tested in a multilayer model of vaginal mucosa, and then, in a preliminary clinical observation to explore its capacity to preserve vaginal mucosal integrity in women in peri-menopause. In keratinocytes cells, perilla phytocomplex demonstrated to exert a marked activity in epidermis barrier maintenance and anti-inflammatory effects, preserving tight junction expression and downregulating cytokines release through targeting JNK activation. Furthermore, perilla showed positive effects in retaining vaginal mucosal integrity in the reconstructed vaginal mucosa model and in vivo tests. Overall, our data suggest that the biotechnological P. frutescens phytocomplex could represent an innovative ingredient for dermatological applications.
Dissertations / Theses on the topic "Phytocomplex"
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VASARRI, MARZIA. "Biochemical investigation on the bioactive properties of the Posidonia oceanica (L.) Delile marine plant." Doctoral thesis, 2021. http://hdl.handle.net/2158/1235961.
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Posidonia oceanica (L.) Delile is an endemic marine plant of the Mediterranean Sea of great ecological importance. Although the literature tells of a millennial relationship between P. oceanica and humans and despite the tradition has handed down the use of this seagrass as a natural herbal remedy, the potential benefits of P. oceanica for human health are poorly documented.
The research group of Prof. Degl'Innocenti of the Department of Biomedical, Experimental and Clinical Sciences "Mario Serio" (University of Florence) was among the first to explore the bioactivities of a hydroalcoholic extract from P. oceanica leaves (POE), shedding light on its efficacy and non-toxic nature in cell-based experimental models.
The possibility of working with a mixture of compounds of marine origin able to act synergistically without leaving signs of cellular toxicity has aroused my interest in continuing to explore the bioactive properties of POE with particular reference to mechanisms and molecular interactions and targeted signalling pathways underlying its activity, with the future prospective of exploring its potential benefits, hitherto unexplored, for human health.
During my research activity, I have achieved excellent results related to the ability of POE to inhibit the migration of cancer cells (such as human fibrosarcoma HT1080 cells and human neuroblastoma SH-SY5Y cells) without any effects on cell viability. A more in-depth investigation of the mechanism of action of POE revealed that the phytocomplex controlled cell migration process through the activation of a transient autophagic flux. During my research, nanotechnology was also investigated as an innovative system for the nano-delivery of phytocomplexes as well. In particular, Soluplus® polymeric nanomicelles proved to be an exceptional delivery system capable of increasing the aqueous solubility of POE and bio-enhancing its anti-migratory ability on SH-SY5Y cells.
In light of a tradition handed down in Anatolia on the antidiabetic role of P. oceanica, confirmed by an in vivo study in animal models, my research activity investigated the properties of POE as an antioxidant, anti-inflammatory and anti-glycation agent. In fact, it is well known that oxidative stress and inflammation are among the factors characterizing the pathophysiology of diabetes, while protein glycation is a crucial event responsible for the onset of diabetics complications under chronic hyperglycemia. The results obtained with my research help to give a more complete picture of the POE molecular mechanisms of action by shedding light on its potential benefits in the management of chronic diseases such as diabetes and related complications, but also of other inflammatory-related diseases, including cancer.
So, overall my mainly cell-based experimental research used a marine resource of extreme environmental importance to explore it in terms of bioactivity from a biochemical point of view, highlighting that the P. oceanica phytocomplex is undoubtedly a promising resource of effective and non-toxic bioactive compounds.
My research also established POE bioactivities also in in vivo animal models, opening new research perspectives concerning the development of alternative and complementary POE-based therapeutic strategies against various diseases.
This research activity carried out during the three years of the PhD is disclosed in papers published in international peer-reviewed scientific journals.
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Detti, Cassandra. "New insights on the diversity of phytocomplexes from Mediterranean shrubs species and their potential nutraceutical and industrial applications." Doctoral thesis, 2022. https://hdl.handle.net/2158/1279479.
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Mediterranean plants are exposed to different environmental stresses, such as high temperatures, solar irradiance, and low water availability, that coexist together, especially during the summer period. To cope with such a combination of stresses, these plants have developed a suite of morpho-anatomical, physiological, and biochemical mechanisms. In particular, they increase the production of secondary metabolites with many defensive functions. Among them, polyphenolic compounds play an important ecological role, acting as UV screeners and antioxidants against reactive oxygen species (ROS) which are produced when the photosynthetic metabolism is impaired by abiotic constraints.
The aim of this PhD project was to investigate four native Mediterranean wild species (Arbutus unedo L., Cistus x incanus L., Myrtus communis L. and Pistacia lentiscus L.) as sources of phytocomplexes that can be exploited for nutraceutical and other industrial applications. We have planned the set-up of green extractive methods to obtain polyphenolic rich extracts from their leaves and fruits, to perform a field-experiment for enhancing the polyphenolic production, and to test the potential utilization of these phytocomplexes as nutraceuticals.
To achieve this, the initial part of the project was the setting up of efficient ultrasound-assisted (UAE) extractions using the Response Surface Methodology. In particular, a fractional factorial screening was performed to verify the effects of different levels of temperature, solvent volume, percentage of ethanol and time followed by an optimization step using a Box-Behnken design. Then, a field-experiment for enhancing the leaf polyphenolic and arbutin yield in A. unedo plants was performed through the application of water stress on a seasonal basis. Finally, the potential utilization of A. unedo and M. communis as nutraceuticals sources was also tested through the inclusion of their polyphenolic fruit extracts into whey milk, a dairy waste product.
Regarding the optimization of the extractions, for P. lentiscus, higher content of total polyphenols can be achieved extracting the leaves using 50% of ethanol at 50 °C for 15 minutes with a solvent ratio of 0.13 L g-1. For A. unedo, a higher amount of arbutin was obtained using 75% of ethanol at ambient temperature for 15 minutes with a solvent ratio of 0.06 L g-1. Finally, for C. incanus the highest content of polyphenols was obtained using 60% EtOH at 40 °C for 60 minutes using 0.1 L g-1 solvent proportion.
The results of the field experiment showed that the application of water stress during the cultivation of A. unedo plants triggered the biosynthesis of polyphenolic compounds in the leaves, increasing their content from summer until the beginning of autumn. Finally, polyphenolic fruit extracts of A. unedo and M. communis showed to be suitable for the inclusion in whey, maintaining their high polyphenolic content during storage thus proving their possible application as functional additives in the food industry.
In conclusion, all the selected species provided extracts with high yields of polyphenols to be applied in the pharmaceutical and nutraceutical sectors, thus the cultivation of these plants may represent an alternative source of income for farmers in the Mediterranean drought-stressed areas
Book chapters on the topic "Phytocomplex"
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Buriani, Alessandro, Stefano Fortinguerra, and Maria Carrara. "Clinical Perspectives in Diagnostic-omics and Personalized Medicine Approach to Monitor Effectiveness and Toxicity of Phytocomplexes." In Toxicology of Herbal Products, 385–476. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-43806-1_16.
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"Phytocomplexes versus Single-Entity Drugs." In Herbal Medicines, 169–82. CRC Press, 2016. http://dx.doi.org/10.1201/b11208-12.
Full textConference papers on the topic "Phytocomplex"
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POZDNYAKOVA, Olga G., Galina A. BELAVINA, Alexander N. AVSTRIEVSKIKH, Andrey A. VEKOVTSEV, and Valery M. POZNYAKOVSKY. "Antioxidant Phytocomplex with Antitumor Activity." In XVIII International Scientific and Practical Conference "Modern Trends in Agricultural Production in the World Economy". Sibac, 2020. http://dx.doi.org/10.32743/kuz.agri.2020.1-11.
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KALIMULLIN, Marat I., Sai-Suu S. SADI, Alexander N. AVSTRIEVSKIKH, and Valery M. POZNYAKOVSKY. "Biologically Active Phytocomplex for Correction of Carbohydrate Metabolism Disorders Phytocomplex in the Correction of Carbohydrate Metabolism." In XVIII International Scientific and Practical Conference "Modern Trends in Agricultural Production in the World Economy". Sibac, 2020. http://dx.doi.org/10.32743/kuz.agri.2020.12-18.
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ZhDANOVA, Irina. "Effect of vegetable additives on the state of homeostasis of cows." In Multifunctional adaptive fodder production 29 (77). ru: Federal Williams Research Center of Forage Production and Agroecology, 2022. http://dx.doi.org/10.33814/mak-2022-29-77-178-182.
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The results of feeding vitamin-herbal flour from Rhaponticum carthamoides, Onobrychis arenaria and Trifolium pratense to dairy cows in order to increase resistance to diseases of various genesis are presented. For the period 2016–2021 according to the statistics of the GVI of the Perm Region, in livestock farms of all categories, in adult cattle, various pathologies of a non-infectious nature were recorded in 45% of cases. Modern agriculture of the Perm Territory is developing on an industrial basis. At the same time, at large livestock complexes, animals are overloaded, lack of walking, exclusion of insolation, uncontrolled and unreasonable feeding of mono-feed, etc. hinders the full creation of immunity and negatively affects the physiological state of animals. Therefore, at present, the use of phytocomplexes consisting of basic biologically active substances as feed and biologically active additives is of great interest in the animal husbandry industry of the Perm Region. This will make it possible to introduce into the schemes of prevention and treatment of veterinarians an environmentally friendly means to strengthen the immunity and safety of farm animals, which will improve the quality of the products obtained. The results of the research can be used in the sectors of feeding and fodder production in farms of any form of ownership.