Dissertations / Theses on the topic 'Phospholipase D'
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Brown, Shea Austin. "N-Acylethanolamines and Plant Phospholipase D." Thesis, University of North Texas, 1998. https://digital.library.unt.edu/ark:/67531/metadc279270/.
Full textHodson, Jane E. "Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization." Thesis, University of North Texas, 2002. https://digital.library.unt.edu/ark:/67531/metadc3341/.
Full textMebarek, Azzam Saida. "La Phospholipase D, une voie de signalisation lipidique impliquée dans de multiples fonctions cellulaires : morphologie, prolifération, différenciation." Lyon, INSA, 2006. http://theses.insa-lyon.fr/publication/2006ISAL0003/these.pdf.
Full textPhospholipase D (PLD) hydrolyses phosphatidylcholine of cell membranes in response to a variety of agonists, to generate phosphatidic acid, a second messenger implicated in cell functions such as cytoskeletal reorganization. In L6 skeletal myoblasts induced to differentiate, a short-lived lowering of ceramide levels was followed by a long-lasting elevation due to de novo synthesis. Ceramide mediates a negative control of myogenic differentiation, at least in part through down-regulation of PLD1 isoform expression and inhibition of PLD1-dependent formation of stress fibers. Moreover, we show that PLD is involved in paracellular permeability of endothelial cells through actin cytoskeleton reorganization, and morphological changes. In addition, we show that disruption of membrane lipid rafts by agents specifically active on the lipids of this compartment, induces an activation of PLD and generates anti-proliferative signals in lymphocytes
Rahier-Corticchiato, Renaud. "Caractérisation biochimique des phospholipases D et de leurs domaines fonctionnels : nouvelle méthode de mesure de l’activité phospholipase D." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSE1292/document.
Full textPhospholipase D (PLD) hydrolyses membrane phospholipids, leading to the formation of free polar headgroup and phosphatidic acid releasing, involved in cell signaling. To understand the biochemical properties of PLDs, this work has been made around two axes. The one first concerns the recombinant expression and purification of the PLDa of Arabidopsis thaliana (AtPLDa) in the yeast Pichia pastoris. The N-terminal sequence of the recombinant AtPLDa has been determined and found to lack its first 35 amino acids, suggesting the involvement of a maturing mechanism. However, plant PLDs exhibit a C2-lipid binding domain at their N-terminal region, which is involved in their Ca2+-dependent membrane targeting. Thus, to assess the impact of such a cleavage, whole and mature-like C2 domains of AtPLDa, as well as of AtPLDß, for the sake of comparison were studied. Thus, the characterization of their affinity for phospholipids, combined with their three-dimensional modeling have demonstrated that the differences observed in their regulation by Ca2+, observed between whole and mature-like forms, originated from the presence of a N-terminus amphipathic a helix, removed during the maturation process. The second axis concerns the development of a novel PLD assay that measure PA in a direct, specific and continuous manner, using the chelation enhanced fluorescence property of 8-hydroxyquinoline in the presence of Ca2+. Thus, this assay appears suitable for monitoring both the inhibition of PLDs as well as their substrate specificity, using natural phospholipids with different polar headgroups, and at a microplate scale
Li, Liang. "Regulation of phospholipase D in submandibular glands." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape4/PQDD_0018/NQ53062.pdf.
Full textLee, Jung Hoon. "Suppression of phospholipase D[Alpha] in soybean." Diss., Manhattan, Kan. : Kansas State University, 2008. http://hdl.handle.net/2097/828.
Full textMcKinnon, Murray. "Studies on mammalian phosphatidylcholine specific phospholipase D." Thesis, Open University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.315440.
Full textSkafi, Najwa. "Role of Phospholipase D in Vascular Calcification." Thesis, Lyon, 2017. http://www.theses.fr/2017LYSE1339/document.
Full textVascular calcification is the accumulation of calcium phosphate crystals in blood vessels via a pathological process that resembles physiological bone or cartilage formation. Calcification in the medial layer is mainly seen in diabetic and chronic kidney disease patients. Its main consequence is the loss of elasticity which is indispensable for the function of large arteries. Accordingly, vascular medial calcification was significantly associated with mortality in hemodialysis patients. Vascular calcification treatments are limited to those that correct its causative health problems, but no efficient, specific and targeted interventions are available. Therefore, a deep understanding of its molecular mechanisms is needed to find novel therapeutic targets. Phospholipase D catalyses the hydrolysis of phospholipids into phosphatidic acid and a head group. It is implicated in different cellular functions and diseases. It was found to be activated by factors involved in osteogenesis and others involved in vascular calcification. Thus, we investigated its role in vascular calcification in 3 models: an in-vitro model of murine smooth muscle cell line MOVAS cultured with ascorbic acid and β-glycerophosphate, an ex-vivo model of rat aortas cultured in high phosphate medium, and an in-vivo model of adenine-induced kidney disease in rats in which vascular calcification is induced by further administration of high phosphorus/calcium diet and active vitamin D injections. Calcification was detected in these models using different approaches including alkaline phosphatase activity, calcium dosage, and/or evaluation of osteo-chondrocytic markers expression. Pld1 expression was seen upregulated in all the three models, especially during early stages of calcification, and was accompanied with increased phospholipase D activity in the in-vitro and ex-vivo model. The inhibition of total phospholipase D activity in these two models, or that of phospholipase D1 in case of MOVAS model, abolished calcification. Phospholipase D2-specific inhibition did not induce significant effects. Two pathways by which phospholipase D can be activated were tested, protein kinase C and sphingosine 1-phosphate pathways, but they were found to be involved in calcification but not necessary for phospholipase D activation during this process. Alternatively, the preliminary results showed that PLD may be acting by activation of sphingosine kinase 2 whose activity was found necessary for calcification in the MOVAS model. Further investigations are needed to understand the mechanisms by which phospholipase D is activated and by which it is acting. Phospholipase D could be a novel target for vascular calcification especially that its inhibition in patients did not induce adverse health effects
Arhab, Yani. "Caractérisation structurale et fonctionnelle des phospholipases D." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1225/document.
Full textPhospholipases D (PLD, EC 3.1.4.4) are ubiquitary enzymes found in prokaryotes (bacteria) as well as in eukaryotes (plant, animals and fungi). PLD catalyzes the hydrolysis of the distal phosphoester bound of phospholipids thus forming phosphatidic acid, an important cell signaling messenger implicated in numerous pathways such as cell proliferation, vesicular formation and trafficking but also transcription and cell survival. PLDs belong to a superfamily of protein which share a common catalytic site called “HKD†for HXKX4D, X is a random amino acid, containing H (Histidyl), K (lysyl) and D (aspartyl) residues. This consensus sequence is duplicated in most of the PLD superfamily members. The study of plant PLD is the best way to understand this family of proteins as they are the sole eukaryotic PLDs to be purified to homogeneity so far. This work provides a functional characterization of the most conserved residues in plant PLDs leading to a structural characterization with the crystallization of this enzyme. A second part of this work proposes the modulation of the enzyme hydrolysis activity by studying the minimal domain necessary for the activity and post-translational maturation undergone by plant PLDs. Also, we look for a new specific inhibitory molecule. Finally, we propose the cloning of a new plant PLD and the development of a new way to detect in vivo PLD activity
Heung, Yen Ming Mary. "Molecular selectivity of phospholipase D in granulocyte function." Thesis, University of Southampton, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.241935.
Full textZeiller, Caroline. "Phospholipase D, perméabilité endothéliale, et apoptose TNFα dépendante." Lyon, INSA, 2007. http://theses.insa-lyon.fr/publication/2007ISAL0087/these.pdf.
Full textPLD is a membrane-bound enzyme which plays a key role in lipid metabolism by generating phosphatidic acid, an anionic phospholipid involved in many cellular functions. Two isoforms PLD 1 and PLD2 exist in mammals. We have shown that PLD enhances the permeability of endothelial cell monolayers (HUV-EC-C cells) through an actin reorganization which is characterized by synthesis of stress fibers. PLD2 might be more particularly implicated, because of its sub cellular localization to membrane caveolae. We also studied the role of PLD in Tumor Necrosis Factor alpha (TNFα)-induced apoptosis of ECV304 cells. TNFα, a pleiotropic cytokine, activates both apoptotic and pro-survival signals depending on the cell model. We showed that PLD exerts a protective effect against cell death induced by TNFα in the presence of an inhibitor of protein synthesis, cycloheximide. PLD 1 isoform plays a predominant role in this process. Different mechanisms explaining the protective role of PLD are proposed
Kusner, David John. "Regulation of phospholipase D activity in U937 cells." Case Western Reserve University School of Graduate Studies / OhioLINK, 1994. http://rave.ohiolink.edu/etdc/view?acc_num=case1057945948.
Full textFang, Mimi. "The role of phospholipase d in osteoblasts in response to titanium surfaces." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2008. http://hdl.handle.net/1853/26462.
Full textCommittee Chair: Boyan, Barbara; Committee Member: Eskin, Suzanne; Committee Member: Lobachev, Kirill; Committee Member: Schwartz, Zvi. Part of the SMARTech Electronic Thesis and Dissertation Collection.
Raymond, Frank Damian. "Inositol specific phospholipase D activity : a GPI-cleaving enzyme." Thesis, University College London (University of London), 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321569.
Full textMcCulloch, Derek A. "Activation of phospholipase D by G protein-coupled receptors." Thesis, University of Edinburgh, 1999. http://hdl.handle.net/1842/22467.
Full textPisch-Heberle, Sandra. "Untersuchungen zur Stabilisierung von Membranproteinen mit ungewöhnlichen Phospholipiden." [S.l.] : Universität Stuttgart , Fakultät Chemie , Institut für Technische Biochemie, 2000. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB8619088.
Full textJaafar, Rami. "Régulation des la voie mTOR par la phospholipase D dans le muscle squelettique : implication dans le contrôle de la différenciation myogénique et de la taille des myocytes." Phd thesis, INSA de Lyon, 2011. http://tel.archives-ouvertes.fr/tel-00679827.
Full textGrant, Margaret Rose. "Characterisation of the pleckstrin homology (PH) domain of phospholipase D." Thesis, University of Birmingham, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.397782.
Full textKnapek, Katie J. "The Role of Phospholipase D (PLD) and Grb2 in Chemotaxis." Wright State University / OhioLINK, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=wright1230574811.
Full textGanesan, Ramya. "Phospholipase D: Key Player in Macrophage-mediated Inflammation and Resolution." Wright State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright1516049400177206.
Full textYozwiak, Michael Leo 1963. "Effect of Corynebacterium pseudotuberculosis phospholipase D on ovine neutrophil function." Thesis, The University of Arizona, 1990. http://hdl.handle.net/10150/277329.
Full textTanguy, Emeline. "Implication de l’acide phosphatidique dans le trafic membranaire : rôle et régulation de la phospholipase D au cours de la phagocytose et de l’exocytose régulée." Thesis, Strasbourg, 2019. http://www.theses.fr/2019STRAJ112.
Full textThe discovery of the involvement of lipids in membrane trafficking is one of the major recent progress in cell biology. My thesis work focused on phosphatidic acid (PA), the simplest phospholipid. PA synthesis by phospholipase D (PLD) plays a crucial role during phagocytosis and regulated exocytosis, but its precise dynamics, as well as the mode of action of the different PA species, remain unknown. I characterized three PA binding domains allowing a better understanding of the interaction between proteins and PA and leading to the generation of genetic sensors for PA in cells. Thus I could visualize PA synthesis during phagocytosis and identified that the small GTPase Arf6 regulates PLD activity and consequently PA synthesis. My work also reveals that PLD modulates several steps during exocytosis in neuroendocrine cells. Further lipidomics and rescue experiments allowed me to show that mono- and polyunsaturated forms of PA are involved in distinct steps of exocytosis
Petersen, Gitte. "Characterization and partial purification of N-acylethanolamine phospholipid-hydrolyzing phospholipase D /." Kbh. : Danmarks Farmaceutiske Højskole, 2001. http://www.dfh.dk/phd/defences/gittepedersen.html.
Full textParre, Elodie. "La signalisation lipidique et le métabolisme de la proline en réponse à des contraintes hydriques : rôles des phospholipases C et D chez Arabidopsis thaliana." Paris 6, 2008. http://www.theses.fr/2008PA066213.
Full textFennell, Myles. "Activation of phospholipase D by the LHRH receptor and associated mechanisms." Thesis, University of Edinburgh, 1995. http://hdl.handle.net/1842/28004.
Full textDevlin, Marni Allison. "The characterization of TSH-mediated phospholipase D activity in thyroid cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape9/PQDD_0017/MQ47020.pdf.
Full textFlores, Borja Fabian. "Characterisation of the mouse glycosylphosphatidyl inositol phospholipase D (GPI-PLD) gene." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.404723.
Full textMorgan, Clive Paul. "ARF regulated phospholipase D : role in cell signalling and membrane traffic." Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.288004.
Full textFite, Kristen. "Dysregulation of Phospholipase D (PLD) isoforms increases breast cancer cell invasion." Wright State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=wright149557402792618.
Full textHeller, Manfred. "Characterization of glycosylphosphatidylinositol-specific phospholipase D from mammalian liver and serum /." [S.l.] : [s.n.], 1994. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.
Full textXie, Mingsheng. "The regulation and functional significance of phospholipase D in HL-60 cells." Case Western Reserve University School of Graduate Studies / OhioLINK, 1992. http://rave.ohiolink.edu/etdc/view?acc_num=case1056134184.
Full textRiebeling, Christian. "Mechanisms of regulation of phospholipase D isoforms involvement in differentiation and apoptosis /." [S.l.] : [s.n.], 2001. http://www.diss.fu-berlin.de/2001/100/index.html.
Full textUfer, Guido [Verfasser]. "Proteins under the control of phospholipase D in Arabidopsis thaliana / Guido Ufer." Bonn : Universitäts- und Landesbibliothek Bonn, 2016. http://d-nb.info/1132711495/34.
Full textUddin, Mohib. "Role of priming in the regulation of phospholipase D in human neutrophils." Thesis, University of Cambridge, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.620569.
Full textYamamoto, Izumi. "Studies on Activation and Inhibition of Phospholipase D in Small Unilamellar Vesicles." 京都大学 (Kyoto University), 2000. http://hdl.handle.net/2433/157221.
Full textKyoto University (京都大学)
0048
新制・論文博士
博士(薬学)
乙第10300号
論薬博第624号
新制||薬||183(附属図書館)
UT51-2000-C67
(主査)教授 半田 哲郎, 教授 中川 照眞, 教授 多賀 徹
学位規則第4条第2項該当
McNamara, Peter Joseph. "Attenuation of Corynebacterium pseudotuberculosis by targeted mutagenesis of the phospholipase D gene." Diss., The University of Arizona, 1994. http://hdl.handle.net/10150/186732.
Full textAbdallah, Dina. "Fonctions de la phospholipase D et des récepteurs de la prostaglandine PGE2 durant la maturation des ostéoblastes, le processus de la minéralisation physiologique et la calcification cardiovasculaire." Thesis, Lyon 1, 2014. http://www.theses.fr/2014LYO10152/document.
Full textLipid metabolism affects the maturation and the differentiation of bone cells. The aim of my PhD thesis is to explore two unknown sides of lipid metabolism which are the actions of phospholipase D (PLD) and those of prostaglandin PGE2 receptors during cell differentiation. Human lineage, Saos-2 cells and primary osteoblasts from calvaria of mice were used as cellular models of physiological mineralization. The ex vivo aorta culture under hyperphosphatemia conditions has been used to reproduce the calcification of the aorta, which is an ex vivo model of cardiovascular calcification (CVC). We showed that the expression and the activity of PLD increased in Saos-2 and primary osteoblasts after the fifth day of differentiation while in the aorta under hyperphosphatemia condition, PLD activity increased at the end of the sixth day. PLD inhibitors decreased the activity of alkaline phosphatase (TNAP) in osteoblasts and in calcified aorta while the overexpression of PLD1 in the Saos-2 increased it. In the second part of this work, we monitored the variation of the expression of PGE2 receptors during the maturation of Saos-2 cells. The EP3 gene expression increased in the late stage of the mineralization while that of EP4 decreased. In conclusion, these results indicated that the PLD activity by affecting the activity of TNAP could modulate the physiological mineralization and CVC. We showed that the mineralization is dependent of the change of the expression of PGE2 receptors in Saos-2 cells
Hagele, Thomas. "Mercury activates phospholipase D in vascular endothelial cells Implications for environmental cardiovascular disease /." Connect to resource, 2006. http://hdl.handle.net/1811/6486.
Full textTitle from first page of PDF file. Document formatted into pages: contains 47 p.; also includes graphics. Includes bibliographical references (p. 26-30). Available online via Ohio State University's Knowledge Bank.
Gargett, Caroline Eve, and mikewood@deakin edu au. "Studies of the human lymphocyte P2Z receptor and its activation of phospholipase D." Deakin University, 1997. http://tux.lib.deakin.edu.au./adt-VDU/public/adt-VDU20060727.144101.
Full textYu, Yuanyuan. "Investigation of potential roles of Phospholipase D in Arabidopsis thaliana seed oil accumulation." Thesis, University of British Columbia, 2009. http://hdl.handle.net/2429/14835.
Full textWhitby, Helen Elizabeth. "The role of glycosylphospatidyl inositol phospholipase D (GDI PLD) in type one hypersensitivity." Thesis, University College London (University of London), 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.251585.
Full textPlanat, Valérie. "Étude des voies de transduction régulant l'activité phospholipase D dans le neutrophile humain." Toulouse 3, 1996. http://www.theses.fr/1996TOU30128.
Full textMcHugh, John. "Molecular and biochemical characterization of phospholipase D in cotton (Gossypium hirsutum L) seedlings." Thesis, University of North Texas, 2005. https://digital.library.unt.edu/ark:/67531/metadc4732/.
Full textQin, Chunbo. "Cloning, expression and characterization of multiple forms of phospholipase D in Arabidopsis thaliana /." Search for this dissertation online, 2003. http://wwwlib.umi.com/cr/ksu/main.
Full textBühler, Anja [Verfasser]. "Molecular mechanisms regulating phospholipase C-gamma 2 activity / Anja Bühler." Ulm : Universität Ulm. Fakultät für Naturwissenschaften, 2016. http://d-nb.info/1094889202/34.
Full textSchäffner, Ines. "Identifizierung und rekombinante Herstellung von Phospholipase D-Isoenzymen aus Weisskohl (Brassica oleracea var. capitata)." [S.l. : s.n.], 2001. http://deposit.ddb.de/cgi-bin/dokserv?idn=963174665.
Full textYu, Changhua. "Phospholipase D/phosphatidic acid phosphatase signal transduction pathway in post-infarction congestive heart failure." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp04/mq23565.pdf.
Full textHa, V. L. "The coordinated regulation of phospholipase D by ADP-ribosylation factors and their exchange factors." Thesis, University College London (University of London), 2004. http://discovery.ucl.ac.uk/1446607/.
Full textGuillemain, Isabelle. "Mode d'action des neurokinines et de divers neurotransmetteurs au niveau de la glande parotide de rat. Regulation de la phospholipase c et de la phospholipase d." Paris 11, 1994. http://www.theses.fr/1994PA112303.
Full textCai, Jingfei. "Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C." Thesis, Boston College, 2013. http://hdl.handle.net/2345/3872.
Full textThesis advisor: Eranthie Weerapana
Peripheral proteins from mammals often exhibit multi-domain structures and require metal ions such as calcium as co-factors. This dissertation investigates two types of such proteins -- pulmonary collectins (surfactant proteins A and D) and phosphatidylinositol-specific phospholipase C (PLC) delta1 -- and their interactions with model membranes. One approach to work around the complexity brought upon by such multi-domain protein structure is to use a truncated construct or an isolated single domain. For pulmonary collectins, homotrimers consisting of the neck domain and the carbohydrate recognition domain were used in a novel NMR assay for better understanding of their lipid-specific interactions with the membranes. For PLC delta1, we were particularly interested in the role of the EF-hand domain. The isolated EF-hand domain of PLC delta1 was first used to characterize its interactions with membranes and identify key residues responsible for such interactions. These key residues in the N terminal lobe of the EF-hand domain, either cationic or hydrophobic, were then found to affect the hydrolysis activity of the full-length enzyme. A common role for this region of the PLC in facilitating proper membrane association was thus proposed
Thesis (PhD) — Boston College, 2013
Submitted to: Boston College. Graduate School of Arts and Sciences
Discipline: Chemistry