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Dissertations / Theses on the topic 'Phospholipase C'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Zhou, Yixing Harden T. Kendall. "Cloning and characterization of a novel phospholipase C enzyme, human phospholipase C eta2." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1271.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.<br>Title from electronic title page (viewed Mar. 26, 2008). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Pharmacology." Discipline: Pharmacology; Department/School: Medicine.

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2

Lin, Gialih Hoffmann. "Stereochemistry and mechanism of phospholipase A2 and phosphatidylinositide-specific phospholipase C /." The Ohio State University, 1989. http://rave.ohiolink.edu/etdc/view?acc_num=osu1487672245900381.

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3

Tong, Yun. "Phosphoinositide-phospholipase C in diabetic cardiomyopathy." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0001/MQ32268.pdf.

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4

Zhao, Li. "Mechanistic studies on phosphatidylinositol-specific phospholipase C." Connect to this title online, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1047485476.

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Thesis (Ph. D.)--Ohio State University, 2003.<br>Title from first page of PDF file. Document formatted into pages; contains xix, 135 p.; also includes graphics (some col.) Includes bibliographical references (p. 128-135). Available online via OhioLINK's ETD Center

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5

Donahue, Vicki S. "Phospholipase c activity in retinal pigment epithelium." Virtual Press, 1997. http://liblink.bsu.edu/uhtbin/catkey/1041916.

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The role of the retinal pigment epithelial cells on the viability and renewal of photoreceptors has been well demonstrated in the Royal College of Surgeons (RCS) strain of rat. These rats are characterized by an inherited time-dependent degeneration of their photoreceptors. This degeneration is apparently due to the inability of the retinal pigment epithelial cells to adequately ingest fragments of photoreceptor membrane that are shed during the course of photoreceptor membrane renewal. The buildup of photoreceptor material in the interphotoreceptor space ultimately leads to the degeneration o

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6

Clark, Graeme Christopher. "Characterisation and exploitation of clostridial phospholipase C." Thesis, Birkbeck (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.406120.

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7

Blank, Jonathan Louis. "The phospholipase C specific for the phosphoinositides." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.252943.

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8

Meldrum, Eric. "Isolation and characterization of mammalian phospholipase C." Thesis, Open University, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.258438.

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9

Chrétien, Louise. "Régulation de l'activité de la phospholipase C par les récepteurs AT [indice] 1 de l'angiotensine II et B [indice] 2 de la bradykinine." Sherbrooke : Université de Sherbrooke, 1998.

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10

Nazih, Hassan. "Hdl3, plaquettes et seconds messagers : caracterisation et regulation du systeme de transduction." Lille 2, 1993. http://www.theses.fr/1993LIL2P261.

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11

Popovics, Petra. "Biochemical and functional characterisation of phospholipase C-η2". Thesis, University of St Andrews, 2013. http://hdl.handle.net/10023/3511.

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Phospholipase C enzymes are important cell signalling enzymes that catalyse the cleavage of phosphatidylinositol 4,5-bisphophate PI(4,5)P₂ into two biologically active second messenger molecules. These are the inositol 1,4,5-trisphosphate which initiates Ca²⁺ release from the endoplasmic reticulum and the diacylglycerol that activates protein kinase C. Although this basic function is shared between the different isoforms, the PLC family encompasses a diverse collection of proteins with various domain structures in addition to the PLC-specific domains. The neuron-specific “6th family” of these

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12

Raabe, Andreas Christian. "Rôle de la phospholipase C, des phosphoinositides et du calcium dans la gamétogenèse de Plasmodium berghei, parasite du paludisme." Montpellier 2, 2008. http://www.theses.fr/2008MON20081.

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Une étape cruciale dans le cycle de Plasmodium, parasite causal du paludisme est sa transmission de l'hôte vertébré au moustique vecteur. Seuls les stades sanguins sexués sont capables de se développer dans le moustique. Chez le moustique, le nouvel environnement déclenche une cascade de signalisation conduisant à la gamétogenèse. Les travaux de thèse ont pour objectifs d'élucider cette cascade de signalisation qui joue un rôle clé dans la transmission du paludisme. Des approches pharmacologiques biochimiques et génétiques ainsi que des études d'imagerie ont été utilisées et fournissent de mul

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13

Day, James Edward Harvey. "The Design and Synthesis of Phospholipase C Gamma Inhibitors." Thesis, Institute of Cancer Research (University Of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.487209.

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Phospholipase C (PLC) catalyses the hydrolysis of phosphatidylinositoI4,5-biphosphate to two important secondary messengers which mediate downstream cell motility and cell proliferation. PLCy is of particular interest because of its involvement with growth factor mediated cell motility, which is a key process to both angiogenesis and tumour invasion. Experimental results provide compelling evidence that PLCy may be rate limiting in tumour invasion and angiogenesis. There is a need for efficacious and 'drug like' inhibitors ofPLCy. Two compounds CCT006000 and CCT007740 were identified from a hi

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14

Klein, Ryan Reaves Thakker Dhiren R. "Regulation of tight junction barrier function by phospholipase C." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1380.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.<br>Title from electronic title page (viewed Apr. 25, 2008). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the School of Pharmacy." Discipline: Pharmacy; Department/School: Pharmacy.

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15

Mills, Lewis Nathan. "The role of phospholipase C in guard cell signalling." Thesis, Lancaster University, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.429967.

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16

Blake, Robert A. "Regulation of the platelet phospholipase C by tyrosine phosphorylation." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240551.

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17

Treagus, Jane Elizabeth. "The translocation of phospholipase C-β1 into the nucleus". Thesis, University of Cambridge, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.625027.

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18

Shi, Xiaomeng. "Phosphatidylinositol-specific phospholipase C: Conformational changes upon membrane binding." Thesis, Boston College, 2010. http://hdl.handle.net/2345/1413.

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Thesis advisor: Mary F. Roberts<br>Phosphatidylinositol-specific phospholipase C (PI-PLC) from B. thuringiensis is activated by phosphatidylcholine (PC) surfaces for both phosphatidylinositol (PI) cleavage to inositol 1,2-(cyclic)-phosphate (cIP) and subsequent hydrolysis of cIP to inositol-1-phosphate. These enzyme kinetics strongly suggest that this PI-PLC has two discrete binding sites for phospholipids - the active site binding PI (or substrate competitors) and an activator site specific for PC. However, it is difficult to determine the orientation and conformation of peripheral membrane p

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19

Blachier, François. "Activation de la phospholipase c par trois agents insulinotropes." Paris 6, 1988. http://www.theses.fr/1988PA066085.

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20

Blachier, François. "Activation de la phospholipase C par trois agents insulinotropes." Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb37611958m.

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21

De, Filippis Lidia. "The phospholipase C signalling system : study of the role of the Go protein and identification of a novel variant of Phospholipase C Î²4." Thesis, Open University, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.270087.

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22

Bühler, Anja [Verfasser]. "Molecular mechanisms regulating phospholipase C-gamma 2 activity / Anja Bühler." Ulm : Universität Ulm. Fakultät für Naturwissenschaften, 2016. http://d-nb.info/1094889202/34.

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23

Rezaei, Effat. "Regulation of phospholipase C in human megakaryocyte-like cell lines." Thesis, University of Oxford, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337499.

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24

Hodson, Elizabeth Anne Marie. "G protein regulation of phospholipase C in vascular smooth muscle." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.390487.

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25

Leslie, Dario Lyall. "Genetic analysis of alpha toxin (phospholipase C) from Clostridium perfringens." Thesis, University of Newcastle Upon Tyne, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.346420.

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26

Nomikos, Michail. "Molecular and enzymatic characterization of mammalian phospholipase C zeta (PLCzeta)." Thesis, Cardiff University, 2006. http://orca.cf.ac.uk/55522/.

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In mammalian oocytes, the fertilising sperm evokes intracellular calcium (Ca2+) oscillations that are essential for the initiation of egg activation and embryonic development. Although the exact mechanism leading to the initiation of Ca2+ oscillations is still unclear, accumulating evidence suggests that sperm-specific phospholipase C zeta (PLC zeta) is delivered from the fertilising sperm into the ooplasm, triggering the Ca2+ oscillations through the inositol 1,4,5-trisphosphate (InsP3) pathway. PLC zeta is the smallest known mammalian PLC isoform comprising of two EF hand, a C2 and the X and

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27

Chang, Wei-Chiao. "Ca²+-dependent-regulation of phospholipase A² and leukotriene C⁴ secretion." Thesis, University of Oxford, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.670084.

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28

Jäger, Karin. "Glycosyl-phosphatidylinositol-anchored acetyl-cholinesterase and phosphatidylinositol-specific phospholipase C /." Bern, 1990. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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29

Ladas, Ioannis. "The role of phospholipase C enzymes in health and disease." Thesis, Cardiff University, 2015. http://orca.cf.ac.uk/75723/.

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Phospholipases C (PLCs) are a large family of enzymes that regulate the cleavage of phosphatidylinositol-4,5- bisphosphate into Inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). This project included research on PLCζ and PLCβ1 on male infertility and thyroid cancer respectively. Human PLCζ (hPLCζ) is the key protein for egg activation following sperm-egg fusion and triggers the release of Ca2+ producing a series of oscillations which initiate embryogenesis. Defects in hPLCζ lead to infertility, thus the project aimed at its expression and enrichment suitable for biomedical applicati

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30

Hergenrother, Paul Joseph. "The catalytic mechanism of phospholipase C and the total synthesis of erythromycin B /." Digital version accessible at:, 1999. http://wwwlib.umi.com/cr/utexas/main.

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31

Remmal, Adnane. "Métabolisme des phospholipides dans les plaquettes du rat spontanément hypertendu." Paris 11, 1987. http://www.theses.fr/1987PA112275.

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Afin de rechercher une origine moléculaire du dérèglement de l'homéostasie calcique observé dans la cellule du muscle lisse vasculaire dans l'hypertension primaire, nous avons étudié et comparé le métabolisme des phospholipides membranaire chez le Rat spontanément hypertendu (SHR) et son témoin normo tendu WKY. En raison des nombreuses analogies existantes entre les cellules du muscle lisse vasculaire et les plaquettes sanguines, ces dernières constituent un modèle intéressant pour étudier le métabolisme du calcium chez le sujet hypertendu et pour tenter d'identifier l'anomalie qui pourrait in

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32

Nomura, Wataru. "Methylglyoxal-induced activation of protein kinase C through phospholipase C and TORC2 in Saccharomyces cerevisiae." Kyoto University, 2010. http://hdl.handle.net/2433/120466.

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Kyoto University (京都大学)<br>0048<br>新制・課程博士<br>博士(農学)<br>甲第15423号<br>農博第1808号<br>新制||農||979(附属図書館)<br>学位論文||H22||N4522(農学部図書室)<br>27901<br>京都大学大学院農学研究科応用生命科学専攻<br>(主査)教授喜多恵子, 教授植田和光, 教授阪井康能<br>学位規則第4条第1項該当

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33

Seifert, Jason Paul Harden T. Kendall. "Regulation of phospholipase C-epsilon by Rho and Ras family proteins." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1008.

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Thesis (Ph. D.)--University of North Carolina at Chapel Hill, 2007.<br>Title from electronic title page (viewed Dec. 18, 2007). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Pharmacology." Discipline: Pharmacology; Department/School: Medicine.

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34

Rasonabe, Zinna Marie [Verfasser]. "Intra- and Intermolecular Control of Phospholipase C-gamma / Zinna Marie Rasonabe." Ulm : Universität Ulm, 2016. http://d-nb.info/1104840235/34.

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35

Arastoo, Mohammed. "Characterisation of phospholipase C-η enzymes and their relevance to disease". Thesis, University of St Andrews, 2016. http://hdl.handle.net/10023/15698.

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Phospholipase C enzymes are a class of enzymes that catalyse the cleavage of the membrane phospholipid, phosphatidylinositol bisphosphate (PtdIns(4,5)P₂) into the second messengers, inositol trisphosphate (Ins(4,5)P₃) and diacylglycerol (DAG). Six classes of PLC enzymes have been identified based on their structure and mechanism of activation. PLCηs are the most recently identified family and consist of two isozymes, PLCη1 and PLCη2. The aim of this thesis is to further understand the mechanisms of PLCη activation, the role of PLCη2 in relation to neuritogenesis and their roles in certain dise

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36

Luo, Ding. "Localisation and function of phosphoinositide-specific phospholipase C in Sacchromyces cerevisiae." Thesis, University of Birmingham, 2010. http://etheses.bham.ac.uk//id/eprint/680/.

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Phosphoinositide-specific phospholipase C enzymes (PLCs) cleave the plasma membrane phospholipid PtdIns(4,5)P\(_2\) to generate two messengers, inositol (1,4,5) trisphosphate [Ins(1,4,5)P\(_3\)] and diacylglycerol (DAG). Ins(1,4,5)P\(_3\) is an important second messenger in animal cells. It releases calcium from intracellular stores by opening Ins(1,4,5)P\(_3\) receptors (InsP\(_3\)Rs) and is formed in response to activation of cell surface hormone and growth factor receptors. It is also important for its immediate phosphorylation to form higher phosphorylated inositol phosphates (InsPs), whic

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37

Phillips, Sally Victoria. "Expression of phospholipase c zeta (PLCzeta) in a mammalian cell line." Thesis, Cardiff University, 2009. http://orca.cf.ac.uk/55886/.

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Phospholipase C zeta (PLCzeta) is a sperm specific isoform of phospholipase C. It has been shown to produce a long lasting series of calcium oscillations and triggers the activation of development when introduced into mammalian eggs. It is not known how PLCzeta is regulated or if its effects are specific to eggs. Here Chinese Hamster Ovary (CHO) cells were transfected with cDNA encoding PLCzeta tagged with enhanced yellow fluorescent protein (eYFP) or luciferase (LUC). Comparisons were made between these cells and cells transfected with the catalytically inactive PLCzeta, the corresponding rep

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38

Gellatly, Steven Alexander. "Cloning and characterisation of phospholipase C X-domain containing proteins (PLCXDs)." Thesis, University of St Andrews, 2015. http://hdl.handle.net/10023/7033.

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Members of the phosphoinositide-specific phospholipase C (PI-PLC) enzyme family play a fundamental role in cell signalling pathways by regulating cytosolic calcium and/or the activity of several protein kinases. This thesis reports the identification, molecular cloning and characterisation of a potential seventh sub-class of the PI-PLC enzyme family, the phospholipase C X-domain containing proteins (PLCXDs), which contain only an X domain in their structure. Comparative sequence analysis has identified at least three PLCXD isoforms in the human and mouse genomes (PLCXDs 1, 2 and 3), and at lea

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39

Grebert, Chloé. "Rôle des phospholipases C dans la régulation de la sécrétion d'ions chlorure et de l'homéostasie calcique dans les cellules épithéliales bronchiques : intérêt dans la mucoviscidose." Thesis, Poitiers, 2019. http://www.theses.fr/2019POIT2301.

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Les phospholipases C (PLC) sont des enzymes indispensables dans la transmission des signaux cellulaires via l’hydrolyse des phospholipides membranaires et régulent, par exemple, l’activité de protéines et de canaux ioniques. Les PLC génèrent des seconds messagers tels que le DAG et l’IP3 qui vont respectivement activer la protéine kinase C et augmenter le Ca2+ intracellulaire, deux mécanismes impliquées dans l’activité du canal chlorure CFTR (Cystic Fibrosis Transmembrane conductance Regulator), dont la mutation du gène dans la mucoviscidose est liée à la perte de l’expression et/ou de fonctio

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40

Marc, Sylvie. "Étude de la régulation du système de transduction phospholipase C - inositol phosphates dans le muscle utérin : caractérisation pharmacologique de sous-classes fonctionnelles distinctes de récepteurs muscariniques." Paris 11, 1989. http://www.theses.fr/1989PA112038.

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La première partie de ce travail est une analyse du système de transduction phospholipase C-inositol phosphates dans le muscle utérin de cobaye : a) Dans le myomètre prémarqué au [3H] inositol, la stimulation par le carbachol, l'ocytocine et les prostaglandines conduit à la dégradation de PIP2 avec apparition séquentielle des inositol phosphates dans l'ordre IP3, IP2, IP. B) Une corrélation entre l'efficacité des différents effecteurs pour induire la contraction utérine et l'effet phosphoinositide permet d'impliquer l'inositol triphosphate dans leur action contracturante. C) L'utilisation de N

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41

Parre, Elodie. "La signalisation lipidique et le métabolisme de la proline en réponse à des contraintes hydriques : rôles des phospholipases C et D chez Arabidopsis thaliana." Paris 6, 2008. http://www.theses.fr/2008PA066213.

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Parmi les stratégies adaptatives que les plantes ont développées pour faire face aux contraintes hydriques, l’accumulation de proline est une réponse physiologique fréquemment observée chez un grand nombre d’espèces. Alors que le métabolisme de ce soluté compatible a été relativement bien caractérisé, les voies de signalisation impliquées dans sa régulation restent en grande partie inconnues. Par une approche pharmacologique, nous avons mis en évidence l’existence d’un contrôle positif de la biosynthèse de la proline par les phospholipases C – et la synthèse d’ IP3 – en réponse au NaCl et non

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42

Chen, Wei. "Understanding the kinetic profile of phosphatidylinositol-specific phospholipase C from Listeria monocytogenes." Thesis, Boston College, 2008. http://hdl.handle.net/2345/966.

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Thesis advisor: Mary F. Roberts<br>The phosphatidylinositol-specific phospholipase C (PI-PLC) from Listeria monocytogenes (a monomer in solution) shows unusual kinetic properties compared to other well-studied phospholipases: (i) increased specific activity with decreasing protein concentration, (ii) activation of the phosphotransferase step by salts, and (iii) activation of both the interfacial phosphotransferase and water-soluble phosphodiesterase steps by zwitterionic and neutral amphiphiles. A variety of biophysical studies (fluorescence, NMR, monolayer, vesicle binding) of enzyme/lipid co

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43

Zhao, Li. "Mechanistic Studies on Phosphatidylinositol-specific Phospholitase C." The Ohio State University, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=osu1047485476.

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44

Savopoulos, John William. "Functional characterisation of the pleckstrin homology domain of phospholipase C-#delta#1." Thesis, Institute of Cancer Research (University Of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243307.

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45

Owen, Helen Jane. "Characterisation of a phospholipase C-Î´ from sea urchin eggs and embryos." Thesis, University College London (University of London), 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.411314.

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Cai, Jingfei. "Probing the Membrane Association Mechanisms for Pulmonary Collectins and Mammalian Phospholipase C." Thesis, Boston College, 2013. http://hdl.handle.net/2345/3872.

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Thesis advisor: Mary F. Roberts<br>Thesis advisor: Eranthie Weerapana<br>Peripheral proteins from mammals often exhibit multi-domain structures and require metal ions such as calcium as co-factors. This dissertation investigates two types of such proteins -- pulmonary collectins (surfactant proteins A and D) and phosphatidylinositol-specific phospholipase C (PLC) delta1 -- and their interactions with model membranes. One approach to work around the complexity brought upon by such multi-domain protein structure is to use a truncated construct or an isolated single domain. For pulmonary collecti

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47

Yilmaz, Umut [Verfasser]. "Calcium-abhängige Translokation der Phospholipase C-β1a aus der Plasmamembran / Umut Yilmaz". Berlin : Medizinische Fakultät Charité - Universitätsmedizin Berlin, 2011. http://d-nb.info/1025238826/34.

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48

Reynolds, Nicholas John. "Investigation of phospholipase C/protein kinase C signalling pathways in skin relevant to the pathogenesis of psoriasis." Thesis, Imperial College London, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307375.

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49

盛建中 and Jianzhong Sheng. "Phosphoinositol/Ca2+ pathway in the cardiac k-opioid receptor: physiological role and alternations upontolerance." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1997. http://hub.hku.hk/bib/B31237654.

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50

Yu, Yan Mei. "Effector regulation domains on G[alpha]16 and their role in the activation of phospholipase C[Beta] and other effectors /." View abstract or full-text, 2004. http://library.ust.hk/cgi/db/thesis.pl?BICH%202004%20YU.

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Thesis (M. Phil.)--Hong Kong University of Science and Technology, 2004.<br>Includes bibliographical references (leaves 94-103). Also available in electronic version. Access restricted to campus users.

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