Relevant bibliographies by topics / Phage T3

Academic literature on the topic 'Phage T3'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Phage T3"

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Serwer, Philip, Elena T. Wright, Jorge De La Chapa, and Cara B. Gonzales. "Basics for Improved Use of Phages for Therapy." Antibiotics 10, no. 6 (June 16, 2021): 723. http://dx.doi.org/10.3390/antibiotics10060723.

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Blood-borne therapeutic phages and phage capsids increasingly reach therapeutic targets as they acquire more persistence, i.e., become more resistant to non-targeted removal from blood. Pathogenic bacteria are targets during classical phage therapy. Metastatic tumors are potential future targets, during use of drug delivery vehicles (DDVs) that are phage derived. Phage therapy has, to date, only sometimes been successful. One cause of failure is low phage persistence. A three-step strategy for increasing persistence is to increase (1) the speed of lytic phage isolation, (2) the diversity of phages isolated, and (3) the effectiveness and speed of screening phages for high persistence. The importance of high persistence-screening is illustrated by our finding here of persistence dramatically higher for coliphage T3 than for its relative, coliphage T7, in murine blood. Coliphage T4 is more persistent, long-term than T3. Pseudomonas chlororaphis phage 201phi2-1 has relatively low persistence. These data are obtained with phages co-inoculated and separately assayed. In addition, highly persistent phage T3 undergoes dispersal to several murine organs and displays tumor tropism in epithelial tissue (xenografted human oral squamous cell carcinoma). Dispersal is an asset for phage therapy, but a liability for phage-based DDVs. We propose increased focus on phage persistence—and dispersal—screening.
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Sarkar, Probir, Dola Sengupta, Shantanu Basu, and Umadas Maitra. "Nucleotide sequence of a major class-III phage-T3 RNA-polymerase promoter located at 98.0% of phage-T3 genetic map." Gene 33, no. 3 (January 1985): 351–55. http://dx.doi.org/10.1016/0378-1119(85)90243-4.

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Pajunen, Maria, Saija Kiljunen, and Mikael Skurnik. "Bacteriophage φYeO3-12, Specific forYersinia enterocolitica Serotype O:3, Is Related to Coliphages T3 and T7." Journal of Bacteriology 182, no. 18 (2000): 5114–20. http://dx.doi.org/10.1128/jb.182.18.5114-5120.2000.

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Bacteriophage φYeO3-12 is a lytic phage of Yersinia enterocolitica serotype O:3. The phage receptor is the lipopolysaccharide O chain of this serotype that consists of the rare sugar 6-deoxy-l-altropyranose. A one-step growth curve of φYeO3-12 revealed eclipse and latent periods of 15 and 25 min, respectively, with a burst size of about 120 PFU per infected cell. In electron microscopy φYeO3-12 virions showed pentagonal outlines, indicating their icosahedral nature. The phage capsid was shown to be composed of at least 10 structural proteins, of which a protein of 43 kDa was predominant. N-terminal sequences of three structural proteins were determined, two of them showing strong homology to structural proteins of coliphages T3 and T7. The phage genome was found to consist of a double-stranded DNA molecule of 40 kb without cohesive ends. A physical map of the phage DNA was constructed using five restriction enzymes. The phage infection could be effectively neutralized using serum from a rabbit immunized with whole φYeO3-12 particles. The antiserum also neutralized T3 infection, although not as efficiently as that of φYeO3-12. φYeO3-12 was found to share, in addition to the N-terminal sequence homology, several common features with T3, including morphology and nonsubjectibility to F exclusion. The evidence conclusively indicated that φYeO3-12 is the first close relative of phage T3 to be described.
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Ikebe, Tadayoshi, Akihito Wada, Yoshishige Inagaki, Kumiko Sugama, Rieko Suzuki, Daisuke Tanaka, Aki Tamaru, et al. "Dissemination of the Phage-Associated Novel Superantigen Gene speL in Recent Invasive and Noninvasive Streptococcus pyogenes M3/T3 Isolates in Japan." Infection and Immunity 70, no. 6 (June 2002): 3227–33. http://dx.doi.org/10.1128/iai.70.6.3227-3233.2002.

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ABSTRACT In Japan, more than 10% of streptococcal toxic shock-like syndrome (TSLS) cases have been caused by Streptococcus pyogenes M3/T3 isolates since the first reported TSLS case in 1992. Most M3/T3 isolates from TSLS or severe invasive infection cases during 1992 to 2001 and those from noninvasive cases during this period are indistinguishable in pulsed-field gel electropherograms. The longest fragments of these recent isolates were 300 kb in size, whereas those of isolates recovered during or before 1973 were 260 kb in size. These 260- and 300-kb fragments hybridized to each other, suggesting the acquisition of an about 40-kb fragment by the recent isolates. The whole part of the acquired fragment was cloned from the first Japanese TSLS isolate, NIH1, and its nucleotide sequence was determined. The 41,796-bp fragment is temperate phage φNIH1.1, containing a new superantigen gene speL near its right attachment site. The C-terminal part of the deduced amino acid sequence of speL has 48 and 46% similarity with well-characterized erythrogenic toxin SpeC and the most potent superantigen, SmeZ-2, respectively. None of 10 T3 isolates recovered during or before 1973 has speL, whereas all of 18 M3/T3 isolates recovered during or after 1992 and, surprisingly, Streptococcus equi subsp. equi ATCC 9527 do have this gene. Though plaques could not be obtained from φNIH1.1, its DNA became detectable from the phage particle fraction upon mitomycin C induction, showing that this phage is not defective. A horizontal transfer of the phage carrying speL may explain the observed change in M3/T3 S. pyogenes isolates in Japan.
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Buchholtz, F., and F. W. Schneider. "Computer simulation of T3 / T7 phage infection using lag times." Biophysical Chemistry 26, no. 2-3 (May 1987): 171–79. http://dx.doi.org/10.1016/0301-4622(87)80020-0.

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Pajunen, Maria I., Saija J. Kiljunen, M. E. Lotta Söderholm, and Mikael Skurnik. "Complete Genomic Sequence of the Lytic Bacteriophage φYeO3-12 of Yersinia enterocolitica Serotype O:3." Journal of Bacteriology 183, no. 6 (March 15, 2001): 1928–37. http://dx.doi.org/10.1128/jb.183.6.1928-1937.2001.

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ABSTRACT φYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the φYeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted φYeO3-12 gene products were >70% identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, φYeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonneiD2371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik, J. Bacteriol. 182:5114–5120, 2000). These findings indicate that φYeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.
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Serwer, Philip, Elena T. Wright, Guimei Yu, and Wen Jiang. "30 Illuminating obscure states of the phage T3 DNA packaging motor." Journal of Biomolecular Structure and Dynamics 33, sup1 (May 18, 2015): 17–18. http://dx.doi.org/10.1080/07391102.2015.1032570.

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Serwer, Philip, Elena T. Wright, Borries Demeler, and Wen Jiang. "States of phage T3/T7 capsids: buoyant density centrifugation and cryo-EM." Biophysical Reviews 10, no. 2 (December 14, 2017): 583–96. http://dx.doi.org/10.1007/s12551-017-0372-5.

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Krüger, D. H., Sigrid Hansen, and Cornelia Schroeder. "Virus adaptation to host cells: The non-classical modification of phage T3." Zeitschrift für allgemeine Mikrobiologie 20, no. 8 (January 24, 2007): 495–502. http://dx.doi.org/10.1002/jobm.19800200803.

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Carrascosa, José L., José M. Valpuesta, and Hisao Fujisawa. "Three-Dimensional Structure of Cloned T3 Connector Protein at 1.6nm Resolution." Proceedings, annual meeting, Electron Microscopy Society of America 48, no. 1 (August 12, 1990): 282–83. http://dx.doi.org/10.1017/s0424820100180161.

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The head to tail connector of bacteriophages plays a fundamental role in the assembly of viral heads and DNA packaging. In spite of the absence of sequence homology, the structure of connectors from different viruses (T4, Ø29, T3, P22, etc) share common morphological features, that are most clearly revealed in their three-dimensional structure. We have studied the three-dimensional reconstruction of the connector protein from phage T3 (gp 8) from tilted view of two dimensional crystals obtained from this protein after cloning and purification.DNA sequences including gene 8 from phage T3 were cloned, into Bam Hl-Eco Rl sites down stream of lambda promotor PL, in the expression vector pNT45 under the control of cI857. E R204 (pNT89) cells were incubated at 42°C for 2h, harvested and resuspended in 20 mM Tris HC1 (pH 7.4), 7mM 2 mercaptoethanol, ImM EDTA. The cells were lysed by freezing and thawing in the presence of lysozyme (lmg/ml) and ligthly sonicated. The low speed supernatant was precipitated by ammonium sulfate (60% saturated) and dissolved in the original buffer to be subjected to gel nitration through Sepharose 6B, followed by phosphocellulose colum (Pll) and DEAE cellulose colum (DE52). Purified gp8 appeared at 0.3M NaCl and formed crystals when its concentration increased above 1.5 mg/ml.
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Dissertations / Theses on the topic "Phage T3"

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張順福. "Interactions between Escherichia coli thioredoxin and the DNA polymerase of phage T3/T7." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/90876724721365418867.

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Books on the topic "Phage T3"

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Cat, Collins Big. Phase 2 Set 1 Support F T3: Phase 2. HarperCollins Publishers Limited, 2022.

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Brêtas, Anilce de Araújo. Balanço eletrolítico estratégico na ração de suínos criados em clima quente. Brazil Publishing, 2021. http://dx.doi.org/10.31012/978-65-5861-318-3.

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The effect of Dietary Electrolyte Balance (EB) in rations for growing and finished swine under high temperatures ambient was evaluated. Two hundred male castrated pigs with initial average 25,3±1.3 kg were allotted in completely randomized experimental design with five treatments T1 diet without supplemented electrolyte 191 mEq/kg; T2 (supl B) diet supplementd with sodium bicarbonate (NaHCO3) 250 mEq/kg; T3 (supl B+C) diet supplemented with (NaHCO3) and potassium chloride (KCl) 250 mEq/kg; T4 (supl B) diet supplemented with (NaHCO3) 300 mEq/kg; T5 (supl B+C) diet supplemented (NaHCO3) and (KCl) 300 mEq/kg and four replication and 10 pigs per experimental unit. The average weight of the animals was 68,8±3,4 kg. The performance parameters evaluated were the feed intake (FI), the daily gain (DG), finished weight (FW), nitrogen intake (NI), lysine intake (LI), efficiency of N utilization for weight gain (ENUWG), efficiency of L utilization for weight gain (ELUWG) and the gain:feed ration and physiologic parameters to respiratory frequency (RF) and rectal temperature (RT). Blood was colleted to measure serum concentration of Na, Cl and K. The average temperature was 29,65±1,80ºC with (RU) of 69,6±10,4%. The levels of EB decreased (P<0,05) the (FI) and improved (NI). The others performance parameters evaluated weren’t influenced by treatments (P>0,05). In the termination phase the supplementation with sodium bicarbonate and or potassium chloride with 250 or 300 mEq/kg can be used to correct electrolyte balance under heat stress.
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Book chapters on the topic "Phage T3"

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"T7 Phage (T3 is related)." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics, 1925. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_16611.

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Conference papers on the topic "Phage T3"

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Wang, X. M., Hong Yu Bai, G. F. Xu, Q. Y. Zhang, and M. Zhuang. "Application of Oil-Gas Hybrid Bearing Turbine-Expander in EAST Helium Cryogenic System." In 18th International Conference on Nuclear Engineering. ASMEDC, 2010. http://dx.doi.org/10.1115/icone18-29146.

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Fusion energy with much more advantages could become a serious option for the future energy. The EAST (Experimental Advanced Superconducting Tokomak) device is aiming to study the physical and technical issues involved in stead-state tokamak nuclear fusion. Cryogenic system is one of the most important sub-systems in EAST device, and turbine-expanders are the key components of cryogenic system. In this paper, turbine-expanders with specific configuration in EAST cryogenic system were introduced, and the problem of instability of turbine-expanders in actual refrigerating process was pointed out. Three different refrigerating methods (Using T3, No-using T3 and using a two-phase turbine) were analyzed, and the results showed that the instability of turbine-expander, especially the coldest turbine (T3), will influence the normal operation, even the whole physical experiment of EAST device. The liquefaction capacity will be increased largely by using a two-phase turbine-expander instead of the JT-throttling.
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Sansoucy, E., B. Jodoin, G. E. Kim, and A. L. Moran. "Mechanical Characteristics of Al-Co-Ce Coatings Produced by the Cold Spray Process." In ITSC2007, edited by B. R. Marple, M. M. Hyland, Y. C. Lau, C. J. Li, R. S. Lima, and G. Montavon. ASM International, 2007. http://dx.doi.org/10.31399/asm.cp.itsc2007p0102.

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Abstract Gas atomized feedstock particles of an Al-13Co-26Ce alloy system were sprayed using the Cold Spray deposition technique. The microstructures of the coatings produced are examined and the mechanical characteristics, in particular the bending fatigue and the bond strength, of the Al-Co-Ce coatings are reported. The results show that the Al-Co-Ce coating improved the fatigue behavior of AA 2024-T3 specimens when compared to uncoated and Al clad specimens. During the bond strength tests, the bonding agent failed and no delamination of the coating from the substrate occurred. The microstructural features of the feedstock powder were also found in the coatings. The coatings contained amorphous and crystalline phase contents similar to the ones found in the feedstock powder. It is suggested that the increase in the fatigue properties can be attributed to the residual compressive stresses induced in the coatings and to the high adhesion strength of the coatings to the substrates.
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Mneimneh, Farah, Nesreen Ghaddar, Kamel Ghali, Charbel Moussalem, and Ibrahim Omeis. "Experiment Study for Evaluation of Phase Change Material Cooling Vest’s Effectiveness at Two Melting Points Used by People With Paraplegia During Exercise." In ASME 2020 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/imece2020-23083.

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Abstract Phase change material (PCM) cooling vests were tested on people with thoracic (T1-T12) spinal cord injury (SCI), also called people with paraplegia (PA), during exercise in heat. The purpose was to reduce heat stress, limit the increase in core temperature, and improve thermal comfort for PA under high metabolic rates and hot ambient conditions. This health risk was a result of thoracic SCI and disruption of thermoregulatory responses in PA. The current study aims to evaluate the efficacy of cooling vest on PA during arm-crank exercise at two melting points, 20°C (V20) and 14°C (V14) compared to no vest test (NV). Eleven participants with high- (T1-T3) and mid-thoracic SCI (T4-T8) were selected to participate in three tests. Core and skin temperatures and heart rate values were measured during 15-min precondition, 30-min exercise and 15-min recovery. Subjective voting of thermal comfort, sensation, skin wettedness and perceived exertion were recorded during exercise only. The main findings revealed significant reduction in change in core temperature (0.42±0.3°C;0.38±0.2°C) in V20 and V14 compared to NV tests for mid-thoracic group. For high-thoracic group, V20 and V14 were least effective in reducing core temperature (p &gt; 0.05). Improvements in thermal comfort was established when using V14 and V20 compared to NV by 85% and 30% for high-thoracic group and by 72% and 53% for mid-thoracic group.
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