Dissertations / Theses on the topic 'Pertussis'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Pertussis.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Rambow-Larsen, Amy Alison. "Assembly and secretion of pertussis toxin by bordetella pertussis." Cincinnati, Ohio : University of Cincinnati, 2003. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=ucin1066417734.
Full textRAMBOW-LARSEN, AMY ALISON. "ASSEMBLY AND SECRETION OF PERTUSSIS TOXIN BY BORDETELLA PERTUSSIS." University of Cincinnati / OhioLINK, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1066417734.
Full textHegerle, Nicolas. "Evolution of Bordetella pertussis and Bordetella parapertussis under acellular Pertussis vaccine pressure : What future for whooping cough and Pertussis vaccination ?" Paris 7, 2014. http://www.theses.fr/2014PA077038.
Full textWhooping cough is an acute respiratory disease life threatening for unvaccinated young children. It is caused by Bordetella pertussis and Bordetella parapertussis, two gram-negative bacteria restricted to humans. The introduction of vaccination against B. Pertussis in the 1950s greatly changed the epidemiology of pertussis as well as the bacterial population itself. Whole cell vaccines were first introduced for children immunization and first booster and enabled to control isolates similar to strains included in the vaccines. Acellular pertussis vaccines, only targeting few bacterial antigens, were later introduced for adolescent booster vaccination before being generalized to the whole population, including adults and new-borns. In addition to a change in the type of vaccine-induced immunity, B. Pertussis also had to face increased herd immunity. Few years alter acellular pertussis vaccine introduction we demonstrated a temporal increase in the prevalence of isolates lacking the production of one vaccine antigen, pertactin, while the population remained quite monomorphic at the genetic level as compare to the post-whole cell vaccine era (allelic stability of known virulence factors). We characterized these isolates at the phenotypic level and demonstrated that they remain as virulent in different in vitro and in vivo models of host pathogen interaction while they might present a selective advantage in an acellular immunized background targeting pertactin. Although vaccines remain effective, surveillance must continue to follow the evolution of these isolate prevalence and the possible impact of pertactin loss on vaccine effectiveness
Phillips, Linda Jane. "Immunization against Bordetella pertussis." Thesis, Kansas State University, 1985. http://hdl.handle.net/2097/9871.
Full textSmith, Maureen. "Studies on the modification of insulin secretion by pertussis vaccine and pertussis toxin." Thesis, University of Strathclyde, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303295.
Full textSmith, Colin J. "Genetic studies with Bordetella pertussis." Thesis, University of Glasgow, 1986. http://theses.gla.ac.uk/1505/.
Full textLewandowski, Anna Zofia. "Antigenic variation in Bordetella pertussis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/MQ60480.pdf.
Full textSidey, Fiona M. "Metabolic effects of Bordetella pertussis." Thesis, University of Strathclyde, 1987. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=20352.
Full textLadant, Daniel. "L'adenyl cyclase de bordetella pertussis." Paris 7, 1989. http://www.theses.fr/1989PA077200.
Full textGoard, Jody Ruth. "An Ecological Perspective on Pertussis." ScholarWorks, 2016. https://scholarworks.waldenu.edu/dissertations/2537.
Full textGOYARD, SOPHIE. "Regulation de l'adenylcyclase de bordetella pertussis." Paris 6, 1993. http://www.theses.fr/1993PA066107.
Full textTallett, April. "Structure and function of pertussis toxin." Thesis, University of Edinburgh, 1993. http://hdl.handle.net/1842/20238.
Full textAdvani, Abdolreza. "Epidemiological characterisation of Bordatella pertussis in Sweden, 1970-2004 /." Stockholm, 2007. http://diss.kib.ki.se/2007/978-91-7357-052-7/.
Full textdel, Valle-Mendoza Juana, Wilmer Silva-Caso, Miguel Angel Aguilar-Luis, Valle-Vargas Cristina del, Erico Cieza-Mora, Johanna Martins-Luna, Ronald Aquino-Ortega, Andrea Silva-Vásquez, Jorge Bazán-Mayra, and Pablo Weilg. "Bordetella pertussis in children hospitalized with a respiratory infection: clinical characteristics and pathogen detection in household contacts." BioMed Central Ltd, 2018. http://hdl.handle.net/10757/624653.
Full textThis work was supported by fourth research incentive of the Universidad Peruana de Ciencias Aplicadas (UPC), Lima‑Peru.
Revisión por pares
Leusch, Mark Steven. "Purification and characterization of adenylate cyclase toxin from Bordetella pertussis." Diss., The University of Arizona, 1990. http://hdl.handle.net/10150/184998.
Full textSeabrook, Richard N. "An immunochemical study of B. pertussis antigens." Thesis, Queen Mary, University of London, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.279600.
Full textCraig, Frank Furlong. "Effects of Bordetella pertussis on neutrophil leukocytes." Thesis, University of Glasgow, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236023.
Full textLobban, Margaret D. "The structure and function of pertussis toxin." Thesis, University of Edinburgh, 1990. http://hdl.handle.net/1842/19057.
Full textCambuy, Diego Duque. "Epidemiologia genômica de Bordetella pertussis no Brasil." reponame:Repositório Institucional da FIOCRUZ, 2014. https://www.arca.fiocruz.br/handle/icict/13349.
Full textFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil
A coqueluche, ou pertússis, é uma doença do trato respiratório causada principalmente pela bactéria Bordetella pertussis. Após 50 anos de vacinação, pertussis reemergiu, passando a ser a doença imunoprevinível mais frequente mesmo em países desenvolvidos. Várias são as hipóteses para a reemergência de pertússis, uma delas é a adaptação do patógeno frente à vacinação. Linhagens contemporâneas de B. pertussis diferem de linhagens do período pré-vacinal, especialmente em genes codificadores de proteínas usadas na produção de vacinas acelular. Esta re-emergência também tem sido observada no Brasil, assim, realizamos a caracterização genética por MLST baseado nesses genes, de 26 isolados B. pertussis de surtos de três regiões brasileiras (Norte, Sul e Nordeste). Foram identificados dois perfis alélicos, em 24 isolados: prn2-ptxS1A-fim3B-ptxP3, de surtos (2008-2013) de Alagoas, Pernambuco e Rio Grande do Sul - e o perfil prn2-ptxS1A-fim3A-ptxP3 , em dois isolados de Pará/2004. Análises filogenéticas agruparam esses perfis com isolados do período pós vacinal de outras partes do globo. Deste conjunto, três do perfil mais frequente e um do perfil menos frequente, tiveram seus genomas sequenciados na plataforma GS 454 Junior. A comparação desses genomas com outros genomas de B. pertussis disponíveis em dados públicos não identificou SNPs ou genes únicos que caracterizassem os isolados do Brasil Este estudo desenvolveu uma metodologia que permitiu definir a posição da IS481 nos genomas, e uma delas corresponde a um gene relacionado a regulação da transcrição da família MarR, Análise filogenômica, baseada em 826 SNPs, demonstrou que os isolados recentes do Brasil da linhagem pandêmica que presente em todos os continentes, exceto a África. Foi observado também que as relações filogenéticas inferidas pelo MLST são semelhantes àquelas inferidas quando se utiliza o genoma completo, isso denota a pressão seletiva sobre esses genes. Sendo assim, a cepa utilizada na produção da vacina no Brasil, que apresenta o perfil alélico prn1-ptxS1D - fim3A-ptxP2, pode não ser capaz de gerar uma resposta imune protetora frente às linhagens circulantes no país. Este estudo traz, pela primeira vez, informações genéticas e genômicas de isolados de B. pertussis do Brasil, país que apresenta cobertura vacinal bastante heterogênea, que utiliza, oficialmente, a vacina celular, mas que, também, aplica a vacina acelular. As informações reveladas neste estudo podem auxiliar a tomada de ações para o controle de pertússis no Brasil, além do conhecimento sobre epidemiologia e evolução de B. pertussis
Pertussis more commonly referred as whooping cough is respiratory tract disease mainly caused by the bacteria B. pertussis. After 50 years of vaccination pert ussis remerged, becoming the most frequent vaccine preventable disease in developed countries. Many hypotheses have been proposed for the re - emergence of pertussis, one being the pathogen adaptation in a vaccinated environment. Current pertussis strains ar e different than those from the prevaccination era, especially in genes that code for proteins used in acelluar pertussis vaccines. This re - emergence is also observed in Brazil, therefore we characterized 26 isolates from 3 regions of Brazil (North,South,N ortheast) using an MLST approach based on these genes. We identified two allelic profiles, 24 isolates from the states of Rio Grande do Sul (2008 - 2009), Alagoas (2008 - 2009), Pernambuco (2013) and Pará (2004) presented the prn2 - ptxS1A - fim3B - ptxP3 allelic pr ofile, while 2 isolates from Pará (2004) presented the prn2 - ptxS1A - fim3A - ptxP3 allelic profile. Phylogenetic analysis branch these two allelic profiles along with other post vaccination isolates around the globe. Four isolates, three from the dominant prof ile and one from the less frequent profile, had their genomes completed sequenced on the GS 454 Junior Platform. We compared these genomes with others available in public databases and no SNP or unique genes were identified in the Brazilian genomes. This s tudy also developed a methodology that identifies the location of the repetitive region IS481, and what genes it interrupted. One of them was the MarR transcriptional regulator gene. Phylogenomic analysis based on 826 SNPs revealed that Brazilian B. pertus sis lineages are part of the current pandemic linage present in all continents, except Africa. We also observed that phylogenomic relationships are similar to MLST’s. Therefore, strain used for pertussis vaccine in Brazil, that presents the prn1 - ptxS1D - f im3A - ptxP2 allelic profile, might not be able to induce immune response to the current linage circulating in the country. This is the first study with genetic and genomic informations of B. pertussis isolates in Brazil, which is a country with heterogeneou s vaccine coverage and mixed and has both cellular and acellular vaccine administrated to the population. Information brought with this study can help the decision making on the control of pertussis in Brazil and gives new insights on the epidemiology and evolution of B. pertussis
Hayles, Elizabeth Helen. "The Maternal Pertussis Trial: Best practice for communication of perinatal pertussis booster recommendations and optimisation of the cocooning strategy." Thesis, The University of Sydney, 2015. http://hdl.handle.net/2123/15693.
Full textAlhashmi, Ibrahim. "Live Metabolite-Deficient Bordetella pertussis: Determination of Suitability for use as a Vaccine in Humans using the Pertussis Mouse Model." Thesis, Curtin University, 2012. http://hdl.handle.net/20.500.11937/51705.
Full textLuo, Siding. "A Stochastic Model for the spread of Pertussis." Thesis, Uppsala University, Department of Mathematics, 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-121728.
Full textStenson, Trevor H. "Characterization of Bvg-repressed molecules of Bordetella pertussis." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ34841.pdf.
Full textGlaser, Philippe. "Biologie moleculaire de l'adenylate cyclase de bordetella pertussis." Paris 6, 1988. http://www.theses.fr/1988PA066259.
Full textGlaser, Philippe. "Biologie moléculaire de l'adénylate cyclase de Bordetella pertussis." Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb376139521.
Full textTorvaldsen, Siranda. "The epidemiology and prevention of pertussis in Australia." Thesis, The University of Sydney, 2001. http://hdl.handle.net/2123/808.
Full textTorvaldsen, Siranda. "The epidemiology and prevention of pertussis in Australia." University of Sydney. Paediatrics and Child Health, 2001. http://hdl.handle.net/2123/808.
Full textNeal, Shona Elaine. "Genotypic diversity and epidemiological typing of Bordetella pertussis." Thesis, University of Glasgow, 2004. http://theses.gla.ac.uk/30856/.
Full textDill, Michael T. "Characterization of the Aspartate Transcarbamoylase that is Found in the pyrBC Complex of Bordetella Pertussis." Thesis, University of North Texas, 2001. https://digital.library.unt.edu/ark:/67531/metadc3057/.
Full textNascimento, Ivan Pereira. "Expressão de antígenos de Bordetella pertussis em BCG Recombinante: subunidade 1 da toxina Pertussis e fragmento A da Hemaglutinina Filamentosa (FHA)." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-15022016-163854/.
Full textThe development of combined vaccines constitutes one of the priorities in modem vaccine research. The use of live vectors for heterologous antigen presentation is desirable, as it could eliminate the necessity of several doses to reach a maximum protection and increase vaccine coverage. In this work, the potential of recombinant Mycobacterium bovis BCG (Bacillus Calmette and Guerin) (rBCG), expressing Bordetella pertussis antigens was investigated. The antigens used were the genetically detoxified S1 subunít of pertussis toxin (S1-PT) and the CRD fragment of FHA (Filamentous hemagglutinin. The antigen genes were cloned into mycobacterial expression vectors under control of the upregulated M. fortuitum β-lactamase promoter, pBlaF*, in fusion with the β-lactamase signal sequence. Mice were immunized with rBCG expressing S1-PT and the respective splenocytes induced specific production of INF-γ and low IL-4, characterizing a strong antigen-specific Th1-dominant cellular response. The rSCG-S1 PT induced a low humoral response against PT. Mice immunized with rSCG-S1 PT strains displayed high-level of protection against an intracerebral challenge with live B. pertussis. Animals immunized with rBCG expressing CRD induced anti-FHA antibodies production. Protection induced by the combination of these two strains is being evaluated. A new approach for production of rBCG without the use of antibiotic resistance markers was also investigated, using complementation in auxotrophic BCG. A lysine auxotrophic rSCG strain was transformed with expression vectors containing the complementation gene for lysine and the pertussis antigens: selection of recombinant clones was carried in media without lysine. These constructs allowed steady expression of the antigens and will be evaluated for the induction of an immunological response against pertussis. These strains would be appropriate for clinical evaluation in humans.
Giayetto, Víctor O. "Caracterización de la infección por Bordetella spp. y coqueluche en la provincia de Córdoba." Master's thesis, Giayetto VO. Caracterización de la infección por Bordetella spp. y coqueluche en la provincia de Córdoba [Internet]. Universidad Nacional de Córdoba; 2015 [citado el 14 de febrero de 2020]. Disponible en: https://rdu.unc.edu.ar/handle/11086/6066, 2015. http://hdl.handle.net/11086/6066.
Full textFil: Giayetto, Victor Olegario. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Cátedra de Bacteriología y Virología Médica; Argentina.
Las enfermedades respiratorias, entre las que se encuentra coqueluche producida por Bordetella pertussis (Bp), son consideradas como la primera causa de mortalidad infantil en el mundo. Si bien la introducción de la vacuna produjo un marcado descenso en el número de casos en nuestro país, coqueluche continúa siendo un problema en Salud Pública, ya que han aumentado los casos entre adolescentes y adultos a pesar de décadas de coberturas vacunales adecuadas. Los individuos de estos grupos etarios son considerados el reservorio actual del agente, y a partir de ellos, la infección se transmitiría a los menores de un año, quienes son los individuos más susceptibles a la infección. Existe una enfermedad clínicamente similar a coqueluche, denominada Síndrome Coqueluchoide (SC) asociada a infecciones por Virus Respiratorio Sincicial (VRS), Adenovirus, Influenza A o B, Parainfluenza y Metapneumovirus, Chlamydia spp., Mycoplasma pneumoniae, Bordetella bronchiseptica. Debido a la importancia sanitaria actual de las infecciones producidas por Bordetella spp. como agente etiológico de infecciones respiratorias emergentes en Argentina y en el mundo, el objetivo de este estudio fue caracterizar el perfil clínico y epidemiológico de esta infección en Córdoba, evaluando además, en un porcentaje de la población estudiada, la prevalencia de infecciones respiratorias de etiología viral que pueden cursar con un cuadro clínico similar como es el SC. La totalidad de las muestras de secreciones respiratorias obtenidas por aspirado nasofaríngeo y/o hisopado nasofaríngeo entre el 1º de marzo de 2011 y el 28 de febrero de 2013 se derivaron al Laboratorio Central de la Provincia de Córdoba para el diagnóstico molecular de coqueluche. Se estudió para Bp y Bordetella spp. 2.588 casos sospechosos de coqueluche de pacientes de la provincia de Córdoba y se realizó diagnóstico diferencial para infecciones respiratorias virales utilizando Inmunofluorescencia Directa. Se reportó infección por Bordetella spp. en 11,59% de los casos sospechosos estudiados (300/2.588) y en el 9,16% del total de muestras estudiadas (237/2.588) se confirmó infección por Bordetella pertussis.
Respiratory diseases, including whooping caused by Bordetella pertussis (Bp), are considered the leading cause of infant mortality worldwide. Even though the introduction of the vaccine caused a decrease in cases in our country, whooping cough is still a problem in Public Health since cases in teenagers and adults have increased in spite of decades of adequate vaccination coverage. Individuals belonging to these age groups are considered the current reservoir of the agent, and from them, the infection would be passed on to children younger than one year old, who are the ones most susceptible to the infection. There is a disease clinically similar to whooping cough, named Coqueluchoide Syndrome (CS), associated to infections by Respiratory Syncytial Virus (RSV), Adenovirus, Influenza A or B, Parainfluenza, Metapneumovirus, Chlamydia spp., Mycoplasma pneumoniae, Bordetella bronchiseptica. Due to the current sanitary importance of infections caused by Bordetella spp. as an etiological agent of emerging respiratory infections in Argentina and in the world, it was considered important to analyse the clinical and epidemiological profile of this infection in Córdoba, evaluating also the prevalence of respiratory infections of viral etiology than can develop a clinical picture similar to the CS, in a percentage of the population under study. The totality of the respiratory secretion samples obtained from nasopharyngeal aspiration and/or nasopharyngeal swabbing were studied for Bp y Bordetella spp., and then derived to the Central Laboratory of Córdoba Province for the molecular diagnose of whooping cough between March 1st, 2011 and February 28th, 2013; and differential diagnose for respiratory infections was carried out using Direct Immunofluorescence. Clinical and epidemiological data resulting from the evaluation of 2,588 cases suspected of whooping cough in patients in Córdoba province are presented in this study. 11.59% of the suspected cases studied (300/2,588) reported the infection by Bordetella spp. and 9.16% of the total samples studied (237/2,588) confirmed the infection by Bordetella pertussis.
Dupré, Elian. "La régulation de la virulence chez Bordetella pertussis : BvgS, modèle original de capteur de système à deux composants." Thesis, Lille 2, 2013. http://www.theses.fr/2013LIL2S022/document.
Full textVirulence of Bordetella pertussis, the whooping cough agent, is due to a plethora of virulence factors which expression is regulated by the two-component system BvgAS. BvgA is a classical response regulator and BvgS the sensor. BvgS contains 3 putative sensor domains, 2 periplasmic Venus FlyTrap (VFT), linked through a transmembrane segment to a cytoplasmic PAS domain preceding the histidine-kinase. Signals perceived by those sensor domains are still unknown, but a 37°C temperature is sufficient to maintain the system active under laboratory conditions. This activity can be down-modulated by chemical compounds, such as MgSO4 or nicotinate, which at sufficient concentration allows the bacteria to switch to avirulent phase.We investigated the role of BvgS VFT domains. VFTs are ubiquitous domains composed of 2 lobes linked by a hinge hence forming a cleft where a specific ligand can bind and stabilize the VFT in its closed conformation.BvgS VFT domains were crystalized and form an intricate dimer defining large interfaces between the 4 VFTs. VFT2s are closed without a ligand and VFT1s are opened, artificial closure of these domains via a disulfide bond indicates that this is the active conformation of BvgS. The role of the interfaces was probed by site-directed mutagenesis. A positive signal might originate from the periplasm to be transmitted through the membrane by the interfaces and integrated by a functional coupling between the VFT2s and the helices preceding the membrane, H19.These helices should be continued through the membrane and the cytoplasm to the PAS domain. Pas domains are ubiquitous with a highly conserved structure, a 5 stranded sheet surrounded by helices defining a cavity. Pas domains are involved in a wide variety of physiological processes, depending on their ability to bind a ligand. Some PAS might function without a ligand and could then be signal adaptors or amplifiers.We demonstrated PASBvg was dimeric, confirming the dimeric nature of BvgS. Cavity residues were substituted indicating that integrity of the cavity is necessary to maintain activity and modulation capacity coming from the periplasmic moiety. Ligand binding wasn’t demonstrated but couldn’t be excluded. Some residues are needed for the correct coupling of the PAS domain to its flanking helices and hence signal transmission. Loss of these connections generates a strong destabilization of PASBvg and turns BvgS inactive.A positive signal might come from the periplasmic moiety and shoul be maintaines by the PAS domain, which is in a rigid conformation also allowing the transmission of negative signals
Miller, Reginaldo Assad. "Desenvolvimento de metodologia in vitro para avaliação do fenômeno de sensibilização à histamina induzido pelo Toxina pertussis e vacina pertussis in vivo." reponame:Repositório Institucional da FIOCRUZ, 2008. https://www.arca.fiocruz.br/handle/icict/9265.
Full textMade available in DSpace on 2014-12-22T16:56:35Z (GMT). No. of bitstreams: 2 98.pdf: 3463846 bytes, checksum: 8603b620b9560561e10f307f624ab539 (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2008
Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde
Dentre os efeitos induzidos pela toxina pertussis (TP) em mamíferos, ocupa um lugar de destaque o fenômeno conhecido por sensibilização aos efeitos biológicos e letais da histamina, cuja intensidade e constância proporcionaram o estabelecimento de um ensaio in vivo de controle de qualidade para avaliação da segurança de vacinas contra a pertussis (coqueluche) e tríplice bacteriana contra a difteria, tétano e coqueluche (DTP). O ensaio de sensibilização à histamina (ESH) em camundongos NIH fêmeas mostrou-se altamente sensível à toxina pertussis de referência NIBSC 90/518 (TPR) detectando níveis tão baixos quanto 20 ng de TP/dose. Todas as 5 vacinas DTP de um produtor nacional noESH apresentaram níveis de TP ativa que variaram entre 84 a 147 ng/mL, valores inferiores ao valor limite de 1789 ng/mL obtido para a vacina pertussis de referência NIBSC 66/303 (VPR), logo, todas as vacinas foram aprovadas para uso humano. Embora o ESH tenha sido conclusivo quanto à alta especificidade à TP, o elevado número de animais, no mínimo, 40 por ensaio, acarretando alto custo e o sofrimento dos animais são fatores limitantes que dificultam o uso rotineiro como ensaio de controle de qualidade da vacina DTP. O objetivo do nosso estudo foi desenvolver uma metodologia in vitro em preparações de íleo isolado de cobaias Short Hair fêmeas (250 a 300 g) fornecidas pelo CECAL/FIOCRUZ /Rio de Janeiro para avaliação do fenômeno de sensibilização à histamina pela TPR. Todos os experimentos foram aprovados de acordo com as diretrizes estabelecidas pela CEUA/FIOCRUZ. Curvas concentração-efeito à histamina em íleos isolados de cobaias foram analisadas e os parâmetros de concentração efetiva média(CE50), concentração efetiva máxima (CEmax) e de constante de dissociação no equilíbrio do complexo droga-receptor (Kd) para histamina foram determinados [...].
Among the effects induced by pertussis toxin (PT) in mammalian species, a prominence place is occupied by the phenomenon known as sensitization to the biological and lethal effects of the histamine, whose intensity and constancy promoted the establishment of an in vivo quality control assay to evaluate the safety of the pertussis vaccine (PV) against whooping cough and the triple bacterial vaccine, (DPT) against diphtheria, whooping cough and tetanus. The histamine sensitization assay (HSA) performed with NIH female mice was highly sensitive to reference pertussis toxin NIBSC 90/518 (RPT), detecting levels as low as 20 ng of administered RPT/dose, which caused 50% lethality. All five samples of DPT vaccines from one Brazilian producer presented active PT levels in the range of 84 and 147 ng/ml by the HSA, inferior to the limit value of 1789 ng/mL obtained for reference pertussis vaccine NIBSC 66/303 (RPV), thus all the vaccines were approved for use. Although the HSA has been conclusive in relation to its high specificity for RPT, the large number of mice used (at least 40 per assay) results in high costs and the suffering of the mice are limiting factors that make its routine use as a DPT vaccine quality control assay difficult. The aim of our study was to develop an in vitro methodology in ileum segments from female Short Hair guinea pigs (250-300 g) maintained in the animal facilities of the Oswaldo Cruz Foundation in Rio de Janeiro (FIOCRUZ), Brazil, to evaluate the histamine sensitization phenomenon by RPT. All experiments were approved in accordance with the guidelines of the Committee for Ethics in Animal Use of the FIOCRUZ. Concentration effects curves for histamine in guinea pig ileum were studied and the parameters of mean effective concentration (EC50), maximum effective concentration (ECmax) and dissociation constant of drug-receptor complex (Kd) were determined. No increase in ileum contractile response to histamine was detected in relation to control PBS 4 days after intraperitoneal treatment of guinea pigs with doses and dilutions corresponding to mean histamine sensitization dose (HSD50) obtained in NIH female mice of RPT (40 ng), RPV and of 5 DPT vaccines (0.26 IU). In all the ten assays performed on the experimental group, the data followed normal distribution, the variances were homogeneous and no significant differences occurred between assays. With doses 10 times higher than the HSD50 of RPT (400 ng) and of RPV (2.6 IU), analysis of the data showed the same behavior above. Contrary to the anticipated results, histamine EC50 and Kd values in ileum of guinea pigs treated in vivo with RPT were significantly higher than the control and RPV (p< 0.05) with no alteration in ECmax (p= 0.3672). In vitro 15 min treatment of guinea pig ileum with 30 ng/ml of RPT reduced the ECmax to about half in relation to control (p= 0.0028), with no significant reduction in the mean values of histamine EC50 and Kd (p= 0.09). In contrast, in vitro 15 min treatment of ileum with 40 ng/ml of RPT significantly reduced histamine ECmax (p< 0.0069), EC50 (p= 0.0261) and Kd (p= 0.0479) in relation to control ileum. In vitro 15 min treatment with PBS (390 and 520 µL in 13 mL of Tyrode) did not significantly alter the mean values of histamine EC50 (p=0.4043 and p= 0.1035), ECmax (p= 0.2366 and p= 0.2708) or KD (p= 0.4564 and p= 0.1158) in relation to control without treatment, demonstrating no effect of the control solvent (PBS) on ileum contractile response by histamine. In conclusion, increased histamine sensitization in female guinea pig ileum after in vitro treatment of 30 and 40 ng/ml of RPT was demonstrated.
Tefon, Burcu Emine. "Towards Whole Cell Immunoproteome And Subproteomes Of Bordetella Pertussis." Phd thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614238/index.pdf.
Full textGraf, Anna. "Humorale und zelluläre Immunität nach Pertussis-Auffrischimpfung im Jugendalter." Diss., lmu, 2010. http://nbn-resolving.de/urn:nbn:de:bvb:19-114881.
Full textWoldegeorgis, Fasil. "Analysis on seroepidemiology of pertussis, a multivariate statistical approach." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ36539.pdf.
Full textDemydchuk, Mykhaylo. "Structural investigation of the enzymatic mechanism of pertussis toxin." Thesis, Birkbeck (University of London), 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.497793.
Full textBokhari, Syed Habib. "Characterisation and secretion mechanism of Bordetella pertussis autotransporter proteins." Thesis, University of Glasgow, 2002. http://theses.gla.ac.uk/1507/.
Full textCahill, Edward Sean. "Antigen delivery systems for nasal immunisation against B. pertussis." Thesis, University of Nottingham, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.321455.
Full textFunnell, Simon Gordon Paul. "Mechanisms of colonisation of mammalian tissues by Bordetella pertussis." Thesis, Open University, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.239726.
Full textPearce, Alexandra M. "The structure and immunogenicity of fimbriae from Bordetella pertussis." Thesis, Open University, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.333436.
Full textErnest, Lisa Lorene, and Lisa Lorene Ernest. "Pertussis Cocooning for Alaska: Development of an Educational Brochure." Diss., The University of Arizona, 2016. http://hdl.handle.net/10150/621034.
Full textBassinet, Laurence. "Bordetella pertussis et épithélium respiratoire : aspects biologiques et cliniques." Paris 12, 2004. https://athena.u-pec.fr/primo-explore/search?query=any,exact,990003948590204611&vid=upec.
Full textWhooping cough is a respiratory disease caused by Bordetella pertussis (Bp). Because of waning immunity after vaccination, adult are thought to have been acting as a reservoir for infections in infants, for whom the disease can be serious. We report a pertussis out break among health care workers (HCW) in an hospital with the contamination of 2 adults immunosuppressed patients. Such observations could support the vaccination of HCW. We also studied interactions between human respiratory epithelium and Bp as these cells could constitute a bacterial reservoir in adults. We showed that Bp invades human HTE tracheal apithelial cells line. The invasive capacity of the bacterium is inhibited by the expression of a toxin, the adenylate cyclase-hemolysin (AC-Hly). Interaction of Bp with HTE cells induces the secretion of inflammatory cytokines such as IL-6 by the cells. AC-Hly is responsible of this secretion confirming that this toxin plays an important role in the inflammatory response induced by Bp
Bassinet, Laurence Guiso-Maclouf Nicole. "Bordetella pertussis et épithélium respiratoire aspects biologiques et cliniques /." Créteil : Université de Paris-Val-de-Marne, 2007. http://doxa.scd.univ-paris12.fr:8080/theses-npd/th0394859.pdf.
Full textVersion électronique uniquement consultable au sein de l'Université Paris 12 (Intranet). Titre provenant de l'écran-titre. Bibliogr. : 332 réf.
Van, der Ploeg Ingeborg. "Studies of G-protein coupled receptors using pertussis toxin." Stockholm : Kongl. Carolinska Medico chirurgiska Institutet, 1991. http://books.google.com/books?id=ks1pAAAAMAAJ.
Full textDammers-Müller, Cirsten [Verfasser]. "Schwierigkeiten bei der Diagnostik der Pertussis / Cirsten Dammers-Müller." Köln : Deutsche Zentralbibliothek für Medizin, 2010. http://d-nb.info/1007193131/34.
Full textHormozi, E. Kalantar. "Activation and immunogenicity of Bordetella pertussis adenylate cyclase toxin." Thesis, University of Glasgow, 1997. http://theses.gla.ac.uk/30948/.
Full textKoo, Yoon. "The impact of pertussis toxin on T cell functions." Thesis, Aix-Marseille, 2019. http://www.theses.fr/2019AIXM0077.
Full textPertussis toxin (PTX) is an exotoxin uniquely produced from Bordetella pertussis, a human respiratory tract pathogen causing pertussis disease, also known as whooping cough. The toxin is well described its virulence effects during bacterial infection. Most of these effects are due to ADP-ribosyltransferase activity of the molecule that targets G-protein coupled receptors (GPCR). On the other hand, PTX is an important antigen that provides protection against pertussis disease and a major component of all current pertussis vaccines. There are numerous literatures on PTX about its molecular mechanisms and its role during infection phase. Instead, lack of information on how PTX contributes host’s adaptive immunity has incurred confusion in understanding the immunogenic role of PTX. With intranasal infection model of B. pertussis, we detected the generation of CD4 lung-resident memory T cells (Trm) were depending on PTX exposure. For T cell migration study, PTX is being used to inhibit chemokine response. Because most of chemokine receptors are GPCR, the motility of many immune cells including T cells is easily affected by PTX. T cell migration is a sophisticate phenomenon regulated space-temporally. The results demonstrated, once T cells become activated and effector, are less influenced than inactivated T cells.This thesis reports the impact of PTX on T cells in two parts; 1) Role of PTX in adaptive immune response by in vivo infection system and 2) Influence of PTX on T cell motility by in vitro assays
Сміян, Олександр Іванович, Александр Иванович Смиян, Oleksandr Ivanovych Smiian, Олена Геннадіївна Васильєва, Елена Геннадьевна Васильева, Olena Hennadiivna Vasylieva, О. М. Черняк, Г. С. Зайцева, and О. В. Логвінова. "Епідемічна ситуація щодо кашлюка в м. Суми та Cумській області за 2001-2012 роки." Thesis, Сумський державний університет, 2013. http://essuir.sumdu.edu.ua/handle/123456789/32354.
Full textPavic, Espinoza Ivana, Medina Sandy Bendezu, Alzamora Angella Herrera, Maria J. Pons, Adrian V. Hernández, Valle Mendoza Juana Mercedes Del, and Universidad Peruana de Ciencias Aplicadas (UPC). "High prevalence of Bordetella pertussis in severe acute respiratory infections in hospitalized children under 5 years in Lima, Peru." Universidad Peruana de Ciencias Aplicadas (UPC), 2015. http://hdl.handle.net/10757/582376.
Full textAcute respiratory infections (ARI) are the main cause of morbidity and mortality in children under 5 years worldwide. Bordetella pertussis is a highly contagious bacterium that can cause serious illness, and approximately half of infected infants less than 1 year old are hospitalized. Also, pertussis immunization series is not completed until six months of age, leaving young infants vulnerable to pertussis. In Peru, pertussis is an increasing health problem despite immunization efforts, and the role of B. pertussis in ARI is unknown. We determined the prevalence of B. pertussis among children under 5 years old admitted to Hospital Nacional Cayetano Heredia in Lima with diagnosis of ARI between Jan-2009 and Dec 2010. Epidemiological and clinical features were collected, and presence of B. pertussis was determined by PCR (pertussis toxin and IS481 gene). A total of 596 nasopharyngeal samples among children under 5 years were analyzed. In 114 (19.1%) samples were positive for B. pertussis. 32.5% of sample positive to B. pertussis were diagnosed as viral pneumonia at diagnosis. Importantly, 71.9% of cases were under 12 months of age and 58.8% have been contact with other ARI infected people. Significant differences in clinical symptoms between the total ARI cases and B. pertussis cases were not found. The most frequent symptoms in B. pertussis cases were fever (100%), rhinorrhea 78%, cough 71.9% and respiratory distress 60.5%. One child died due to the infection. B. pertussis cases showed a seasonal distribution with peaks during the months March June and November. This study shows the high prevalence of B. pertussis in infants who were hospitalized due to severe acute respiratory infections in Lima, Peru. Epidemiologic surveillance programs for B. pertussis are essential in the future in Peru