Relevant bibliographies by topics / Peroxisomal matrix proteins

Contents

  1. Journal articles
  2. Dissertations / Theses
  3. Book chapters
  4. Reports

Academic literature on the topic 'Peroxisomal matrix proteins'

Author: Grafiati
Published: 4 June 2021
Last updated: 20 February 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Peroxisomal matrix proteins.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Peroxisomal matrix proteins"

1

South, Sarah T., and Stephen J. Gould. "Peroxisome Synthesis in the Absence of Preexisting Peroxisomes." Journal of Cell Biology 144, no. 2 (January 25, 1999): 255–66. http://dx.doi.org/10.1083/jcb.144.2.255.

Full text
Abstract:
Zellweger syndrome and related diseases are caused by defective import of peroxisomal matrix proteins. In all previously reported Zellweger syndrome cell lines the defect could be assigned to the matrix protein import pathway since peroxisome membranes were present, and import of integral peroxisomal membrane proteins was normal. However, we report here a Zellweger syndrome patient (PBD061) with an unusual cellular phenotype, an inability to import peroxisomal membrane proteins. We also identified human PEX16, a novel integral peroxisomal membrane protein, and found that PBD061 had inactivating mutations in the PEX16 gene. Previous studies have suggested that peroxisomes arise from preexisting peroxisomes but we find that expression of PEX16 restores the formation of new peroxisomes in PBD061 cells. Peroxisome synthesis and peroxisomal membrane protein import could be detected within 2–3 h of PEX16 injection and was followed by matrix protein import. These results demonstrate that peroxisomes do not necessarily arise from division of preexisting peroxisomes. We propose that peroxisomes may form by either of two pathways: one that involves PEX11-mediated division of preexisting peroxisomes, and another that involves PEX16-mediated formation of peroxisomes in the absence of preexisting peroxisomes.
APA, Harvard, Vancouver, ISO, and other styles
2

Voorn-Brouwer, Tineke, Astrid Kragt, Henk F. Tabak, and Ben Distel. "Peroxisomal membrane proteins are properly targeted to peroxisomes in the absence of COPI- and COPII-mediated vesicular transport." Journal of Cell Science 114, no. 11 (June 1, 2001): 2199–204. http://dx.doi.org/10.1242/jcs.114.11.2199.

Full text
Abstract:
The classic model for peroxisome biogenesis states that new peroxisomes arise by the fission of pre-existing ones and that peroxisomal matrix and membrane proteins are recruited directly from the cytosol. Recent studies challenge this model and suggest that some peroxisomal membrane proteins might traffic via the endoplasmic reticulum to peroxisomes. We have studied the trafficking in human fibroblasts of three peroxisomal membrane proteins, Pex2p, Pex3p and Pex16p, all of which have been suggested to transit the endoplasmic reticulum before arriving in peroxisomes. Here, we show that targeting of these peroxisomal membrane proteins is not affected by inhibitors of COPI and COPII that block vesicle transport in the early secretory pathway. Moreover, we have obtained no evidence for the presence of these peroxisomal membrane proteins in compartments other than peroxisomes and demonstrate that COPI and COPII inhibitors do not affect peroxisome morphology or integrity. Together, these data fail to provide any evidence for a role of the endoplasmic reticulum in peroxisome biogenesis.
APA, Harvard, Vancouver, ISO, and other styles
3

Knoops, Kèvin, Rinse de Boer, Anita Kram, and Ida J. van der Klei. "Yeast pex1 cells contain peroxisomal ghosts that import matrix proteins upon reintroduction of Pex1." Journal of Cell Biology 211, no. 5 (December 7, 2015): 955–62. http://dx.doi.org/10.1083/jcb.201506059.

Full text
Abstract:
Pex1 and Pex6 are two AAA-ATPases that play a crucial role in peroxisome biogenesis. We have characterized the ultrastructure of the Saccharomyces cerevisiae peroxisome-deficient mutants pex1 and pex6 by various high-resolution electron microscopy techniques. We observed that the cells contained peroxisomal membrane remnants, which in ultrathin cross sections generally appeared as double membrane rings. Electron tomography revealed that these structures consisted of one continuous membrane, representing an empty, flattened vesicle, which folds into a cup shape. Immunocytochemistry revealed that these structures lack peroxisomal matrix proteins but are the sole sites of the major peroxisomal membrane proteins Pex2, Pex10, Pex11, Pex13, and Pex14. Upon reintroduction of Pex1 in Pex1-deficient cells, these peroxisomal membrane remnants (ghosts) rapidly incorporated peroxisomal matrix proteins and developed into peroxisomes. Our data support earlier views that Pex1 and Pex6 play a role in peroxisomal matrix protein import.
APA, Harvard, Vancouver, ISO, and other styles
4

Walton, P. A., P. E. Hill, and S. Subramani. "Import of stably folded proteins into peroxisomes." Molecular Biology of the Cell 6, no. 6 (June 1995): 675–83. http://dx.doi.org/10.1091/mbc.6.6.675.

Full text
Abstract:
By virtue of their synthesis in the cytoplasm, proteins destined for import into peroxisomes are obliged to traverse the single membrane of this organelle. Because the targeting signal for most peroxisomal matrix proteins is a carboxy-terminal tripeptide sequence (SKL or its variants), these proteins must remain import competent until their translation is complete. We sought to determine whether stably folded proteins were substrates for peroxisomal import. Prefolded proteins stabilized with disulfide bonds and chemical cross-linkers were shown to be substrates for peroxisomal import, as were mature folded and disulfide-bonded IgG molecules containing the peroxisomal targeting signal. In addition, colloidal gold particles conjugated to proteins bearing the peroxisomal targeting signal were translocated into the peroxisomal matrix. These results support the concept that proteins may fold in the mammalian cytosol, before their import into the peroxisome, and that protein unfolding is not a prerequisite for peroxisomal import.
APA, Harvard, Vancouver, ISO, and other styles
5

Szilard, R. K., V. I. Titorenko, M. Veenhuis, and R. A. Rachubinski. "Pay32p of the yeast Yarrowia lipolytica is an intraperoxisomal component of the matrix protein translocation machinery." Journal of Cell Biology 131, no. 6 (December 15, 1995): 1453–69. http://dx.doi.org/10.1083/jcb.131.6.1453.

Full text
Abstract:
Pay mutants of the yeast Yarrowia lipolytica fail to assemble functional peroxisomes. One mutant strain, pay32-1, has abnormally small peroxisomes that are often found in clusters surrounded by membraneous material. The functionally complementing gene PAY32 encodes a protein, Pay32p, of 598 amino acids (66,733 D) that is a member of the tetratricopeptide repeat family. Pay32p is intraperoxisomal. In wild-type peroxisomes, Pay32p is associated primarily with the inner surface of the peroxisomal membrane, but approximately 30% of Pay32p is localized to the peroxisomal matrix. The majority of Pay32p in the matrix is complexed with two polypeptides of 62 and 64 kD recognized by antibodies to SKL (peroxisomal targeting signal-1). In contrast, in peroxisomes of the pay32-1 mutant, Pay32p is localized exclusively to the matrix and forms no complex. Biochemical characterization of the mutants pay32-1 and pay32-KO (a PAY32 gene disruption strain) showed that Pay32p is a component of the peroxisomal translocation machinery. Mutations in the PAY32 gene prevent the translocation of most peroxisome-bound proteins into the peroxisomal matrix. These proteins, including the 62-kD anti-SKL-reactive polypeptide, are trapped in the peroxisomal membrane at an intermediate stage of translocation in pay32 mutants. Our results suggest that there are at least two distinct translocation machineries involved in the import of proteins into peroxisomes.
APA, Harvard, Vancouver, ISO, and other styles
6

Anteghini, Marco, Vitor Martins dos Santos, and Edoardo Saccenti. "In-Pero: Exploiting Deep Learning Embeddings of Protein Sequences to Predict the Localisation of Peroxisomal Proteins." International Journal of Molecular Sciences 22, no. 12 (June 15, 2021): 6409. http://dx.doi.org/10.3390/ijms22126409.

Full text
Abstract:
Peroxisomes are ubiquitous membrane-bound organelles, and aberrant localisation of peroxisomal proteins contributes to the pathogenesis of several disorders. Many computational methods focus on assigning protein sequences to subcellular compartments, but there are no specific tools tailored for the sub-localisation (matrix vs. membrane) of peroxisome proteins. We present here In-Pero, a new method for predicting protein sub-peroxisomal cellular localisation. In-Pero combines standard machine learning approaches with recently proposed multi-dimensional deep-learning representations of the protein amino-acid sequence. It showed a classification accuracy above 0.9 in predicting peroxisomal matrix and membrane proteins. The method is trained and tested using a double cross-validation approach on a curated data set comprising 160 peroxisomal proteins with experimental evidence for sub-peroxisomal localisation. We further show that the proposed approach can be easily adapted (In-Mito) to the prediction of mitochondrial protein localisation obtaining performances for certain classes of proteins (matrix and inner-membrane) superior to existing tools.
APA, Harvard, Vancouver, ISO, and other styles
7

Brown, Trevor W., Vladimir I. Titorenko, and Richard A. Rachubinski. "Mutants of theYarrowia lipolytica PEX23Gene Encoding an Integral Peroxisomal Membrane Peroxin Mislocalize Matrix Proteins and Accumulate Vesicles Containing Peroxisomal Matrix and Membrane Proteins." Molecular Biology of the Cell 11, no. 1 (January 2000): 141–52. http://dx.doi.org/10.1091/mbc.11.1.141.

Full text
Abstract:
pex mutants are defective in peroxisome assembly. The mutant strain pex23-1 of the yeast Yarrowia lipolytica lacks morphologically recognizable peroxisomes and mislocalizes all peroxisomal matrix proteins investigated preferentially to the cytosol. pex23 strains accumulate vesicular structures containing both peroxisomal matrix and membrane proteins. The PEX23 gene was isolated by functional complementation of the pex23-1 strain and encodes a protein, Pex23p, of 418 amino acids (47,588 Da). Pex23p exhibits high sequence similarity to two hypothetical proteins of the yeastSaccharomyces cerevisiae. Pex23p is an integral membrane protein of peroxisomes that is completely, or nearly completely, sequestered from the cytosol. Pex23p is detected at low levels in cells grown in medium containing glucose, and its levels are significantly increased by growth in medium containing oleic acid, the metabolism of which requires intact peroxisomes.
APA, Harvard, Vancouver, ISO, and other styles
8

Ma, Changle, Gaurav Agrawal, and Suresh Subramani. "Peroxisome assembly: matrix and membrane protein biogenesis." Journal of Cell Biology 193, no. 1 (April 4, 2011): 7–16. http://dx.doi.org/10.1083/jcb.201010022.

Full text
Abstract:
The biogenesis of peroxisomal matrix and membrane proteins is substantially different from the biogenesis of proteins of other subcellular compartments, such as mitochondria and chloroplasts, that are of endosymbiotic origin. Proteins are targeted to the peroxisome matrix through interactions between specific targeting sequences and receptor proteins, followed by protein translocation across the peroxisomal membrane. Recent advances have shed light on the nature of the peroxisomal translocon in matrix protein import and the molecular mechanisms of receptor recycling. Furthermore, the endoplasmic reticulum has been shown to play an important role in peroxisomal membrane protein biogenesis. Defining the molecular events in peroxisome assembly may enhance our understanding of the etiology of human peroxisome biogenesis disorders.
APA, Harvard, Vancouver, ISO, and other styles
9

Bascom, Roger A., Honey Chan, and Richard A. Rachubinski. "Peroxisome Biogenesis Occurs in an Unsynchronized Manner in Close Association with the Endoplasmic Reticulum in Temperature-sensitiveYarrowia lipolyticaPex3p Mutants." Molecular Biology of the Cell 14, no. 3 (March 2003): 939–57. http://dx.doi.org/10.1091/mbc.e02-10-0633.

Full text
Abstract:
Pex3p is a peroxisomal integral membrane protein required early in peroxisome biogenesis, and Pex3p-deficient cells lack identifiable peroxisomes. Two temperature-sensitive pex3 mutant strains of the yeast Yarrowia lipolytica were made to investigate the role of Pex3p in the early stages of peroxisome biogenesis. In glucose medium at 16°C, these mutants underwent de novo peroxisome biogenesis and exhibited early matrix protein sequestration into peroxisome-like structures found at the endoplasmic reticulum-rich periphery of cells or sometimes associated with nuclei. The de novo peroxisome biogenesis seemed unsynchronized, with peroxisomes occurring at different stages of development both within cells and between cells. Cells with peripheral nascent peroxisomes and cells with structures morphologically distinct from peroxisomes, such as semi/circular tubular structures that immunostained with antibodies to peroxisomal matrix proteins and to the endoplasmic reticulum-resident protein Kar2p, and that surrounded lipid droplets, were observed during up-regulation of peroxisome biogenesis in cells incubated in oleic acid medium at 16°C. These structures were not detected in wild-type or Pex3p-deficient cells. Their role in peroxisome biogenesis remains unclear. Targeting of peroxisomal matrix proteins to these structures suggests that Pex3p directly or indirectly sequesters components of the peroxisome biogenesis machinery. Such a role is consistent with Pex3p overexpression producing cells with fewer, larger, and clustered peroxisomes.
APA, Harvard, Vancouver, ISO, and other styles
10

Gould, S. J., J. E. Kalish, J. C. Morrell, J. Bjorkman, A. J. Urquhart, and D. I. Crane. "Pex13p is an SH3 protein of the peroxisome membrane and a docking factor for the predominantly cytoplasmic PTs1 receptor." Journal of Cell Biology 135, no. 1 (October 1, 1996): 85–95. http://dx.doi.org/10.1083/jcb.135.1.85.

Full text
Abstract:
Import of newly synthesized PTS1 proteins into the peroxisome requires the PTS1 receptor (Pex5p), a predominantly cytoplasmic protein that cycles between the cytoplasm and peroxisome. We have identified Pex13p, a novel integral peroxisomal membrane from both yeast and humans that binds the PTS1 receptor via a cytoplasmically oriented SH3 domain. Although only a small amount of Pex5p is bound to peroxisomes at steady state (< 5%), loss of Pex13p further reduces the amount of peroxisome-associated Pex5p by approximately 40-fold. Furthermore, loss of Pex13p eliminates import of peroxisomal matrix proteins that contain either the type-1 or type-2 peroxisomal targeting signal but does not affect targeting and insertion of integral peroxisomal membrane proteins. We conclude that Pex13p functions as a docking factor for the predominantly cytoplasmic PTS1 receptor.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Peroxisomal matrix proteins"

1

Spathaky, Jane Mary. "A novel method for the isolation of genes encoding peroxisomal matrix proteins." Thesis, University of Cambridge, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.361693.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Maxwell, Megan Amanda, and n/a. "PEX1 Mutations in Australasian Patients with Disorders of Peroxisome Biogenesis." Griffith University. School of Biomolecular and Biomedical Science, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040219.100649.

Full text
Abstract:
The peroxisome is a subcellular organelle that carries out a diverse range of metabolic functions, including the b-oxidation of very long chain fatty acids, the breakdown of peroxide and the a-oxidation of fatty acids. Disruption of peroxisome metabolic functions leads to severe disease in humans. These diseases can be broadly grouped into two categories: those in which a single enzyme is defective, and those known as the peroxisome biogenesis disorders (PBDs), which result from a generalised failure to import peroxisomal matrix proteins (and consequently result in disruption of multiple metabolic pathways). The PBDs result from mutations in PEX genes, which encode protein products called peroxins, required for the normal biogenesis of the peroxisome. PEX1 encodes an AAA ATPase that is essential for peroxisome biogenesis, and mutations in PEX1 are the most common cause of PBDs worldwide. This study focused on the identification of mutations in PEX1 in an Australasian cohort of PBD patients, and the impact of these mutations on PEX1 function. As a result of the studies presented in this thesis, twelve mutations in PEX1 were identified in the Australasian cohort of patients. The identified mutations can be broadly grouped into three categories: missense mutations, mutations directly introducing a premature termination codon (PTC) and mutations that interrupt the reading frame of PEX1. The missense mutations that were identified were R798G, G843D, I989T and R998Q; all of these mutations affect amino acid residues located in the AAA domains of the PEX1 protein. Two mutations that directly introduce PTCs into the PEX1 transcript (R790X and R998X), and four frameshift mutations (A302fs, I370fs, I700fs and S797fs) were identified. There was also one mutation found in an intronic region (IVS22-19A>G) that is presumed to affect splicing of the PEX1 mRNA. Three of these mutations, G843D, I700fs and G973fs, were found at high frequency in this patient cohort. At the commencement of these studies, it was hypothesised that missense mutations would result in attenuation of PEX1 function, but mutations that introduced PTCs, either directly or indirectly, would have a deleterious effect on PEX1 function. Mutations introducing PTCs are thought to cause mRNA to be degraded by the nonsense-mediated decay of mRNA (NMD) pathway, and thus result in a decrease in PEX1 protein levels. The studies on the cellular impact of the identified PEX1 mutations were consistent with these hypotheses. Missense mutations were found to reduce peroxisomal protein import and PEX1 protein levels, but a residual level of function remained. PTC-generating mutations were found to have a major impact on PEX1 function, with PEX1 mRNA and protein levels being drastically reduced, and peroxisomal protein import capability abolished. Patients with two missense mutations showed the least impact on PEX1 function, patients with two PTC-generating mutations had a severe defect in PEX1 function, and patients carrying a combination of a missense mutation and a PTC-generating mutation showed levels of PEX1 function that were intermediate between these extremes. Thus, a correlation between PEX1 genotype and phenotype was defined for the Australasian cohort of patients investigated in these studies. For a number of patients, mutations in the coding sequence of one PEX1 allele could not be identified. Analysis of the 5' UTR of this gene was therefore pursued for potential novel mutations. The initial analyses demonstrated that the 5' end of PEX1 extended further than previously reported. Two co-segregating polymorphisms were also identified, termed –137 T>C and –53C>G. The -137T>C polymorphism resided in an upstream, in-frame ATG (termed ATG1), and the possibility that the additional sequence represented PEX1 coding sequence was examined. While both ATGs were found to be functional by virtue of in vitro and in vivo expression investigations, Western blot analysis of the PEX1 protein in patient and control cell extracts indicated that physiological translation of PEX1 was from the second ATG only. Using a luciferase reporter approach, the additional sequence was found to exhibit promoter activity. When examined alone the -137T>C polymorphism exerted a detrimental effect on PEX1 promoter activity, reducing activity to half that of wild-type levels, and the -53C>G polymorphism increased PEX1 promoter activity by 25%. When co-expressed (mimicking the physiological condition) these polymorphisms compensated for each other to bring PEX1 promoter activity to near wild-type levels. The PEX1 mutations identified in this study have been utilised by collaborators at the National Referral Laboratory for Lysosomal, Peroxisomal and Related Genetic Disorders (based at the Women's and Children's Hospital, Adelaide), in prenatal diagnosis of the PBDs. In addition, the identification of three common mutations in Australasian PBD patients has led to the implementation of screening for these mutations in newly referred patients, often enabling a precise diagnosis of a PBD to be made. Finally, the strong correlation between genotype and phenotype for the patient cohort investigated as part of these studies has generated a basis for the assessment of newly identified mutations in PEX1.
APA, Harvard, Vancouver, ISO, and other styles
3

Maxwell, Megan Amanda. "PEX1 Mutations in Australasian Patients with Disorders of Peroxisome Biogenesis." Thesis, Griffith University, 2004. http://hdl.handle.net/10072/366184.

Full text
Abstract:
The peroxisome is a subcellular organelle that carries out a diverse range of metabolic functions, including the b-oxidation of very long chain fatty acids, the breakdown of peroxide and the a-oxidation of fatty acids. Disruption of peroxisome metabolic functions leads to severe disease in humans. These diseases can be broadly grouped into two categories: those in which a single enzyme is defective, and those known as the peroxisome biogenesis disorders (PBDs), which result from a generalised failure to import peroxisomal matrix proteins (and consequently result in disruption of multiple metabolic pathways). The PBDs result from mutations in PEX genes, which encode protein products called peroxins, required for the normal biogenesis of the peroxisome. PEX1 encodes an AAA ATPase that is essential for peroxisome biogenesis, and mutations in PEX1 are the most common cause of PBDs worldwide. This study focused on the identification of mutations in PEX1 in an Australasian cohort of PBD patients, and the impact of these mutations on PEX1 function. As a result of the studies presented in this thesis, twelve mutations in PEX1 were identified in the Australasian cohort of patients. The identified mutations can be broadly grouped into three categories: missense mutations, mutations directly introducing a premature termination codon (PTC) and mutations that interrupt the reading frame of PEX1. The missense mutations that were identified were R798G, G843D, I989T and R998Q; all of these mutations affect amino acid residues located in the AAA domains of the PEX1 protein. Two mutations that directly introduce PTCs into the PEX1 transcript (R790X and R998X), and four frameshift mutations (A302fs, I370fs, I700fs and S797fs) were identified. There was also one mutation found in an intronic region (IVS22-19A>G) that is presumed to affect splicing of the PEX1 mRNA. Three of these mutations, G843D, I700fs and G973fs, were found at high frequency in this patient cohort. At the commencement of these studies, it was hypothesised that missense mutations would result in attenuation of PEX1 function, but mutations that introduced PTCs, either directly or indirectly, would have a deleterious effect on PEX1 function. Mutations introducing PTCs are thought to cause mRNA to be degraded by the nonsense-mediated decay of mRNA (NMD) pathway, and thus result in a decrease in PEX1 protein levels. The studies on the cellular impact of the identified PEX1 mutations were consistent with these hypotheses. Missense mutations were found to reduce peroxisomal protein import and PEX1 protein levels, but a residual level of function remained. PTC-generating mutations were found to have a major impact on PEX1 function, with PEX1 mRNA and protein levels being drastically reduced, and peroxisomal protein import capability abolished. Patients with two missense mutations showed the least impact on PEX1 function, patients with two PTC-generating mutations had a severe defect in PEX1 function, and patients carrying a combination of a missense mutation and a PTC-generating mutation showed levels of PEX1 function that were intermediate between these extremes. Thus, a correlation between PEX1 genotype and phenotype was defined for the Australasian cohort of patients investigated in these studies. For a number of patients, mutations in the coding sequence of one PEX1 allele could not be identified. Analysis of the 5' UTR of this gene was therefore pursued for potential novel mutations. The initial analyses demonstrated that the 5' end of PEX1 extended further than previously reported. Two co-segregating polymorphisms were also identified, termed –137 T>C and –53C>G. The -137T>C polymorphism resided in an upstream, in-frame ATG (termed ATG1), and the possibility that the additional sequence represented PEX1 coding sequence was examined. While both ATGs were found to be functional by virtue of in vitro and in vivo expression investigations, Western blot analysis of the PEX1 protein in patient and control cell extracts indicated that physiological translation of PEX1 was from the second ATG only. Using a luciferase reporter approach, the additional sequence was found to exhibit promoter activity. When examined alone the -137T>C polymorphism exerted a detrimental effect on PEX1 promoter activity, reducing activity to half that of wild-type levels, and the -53C>G polymorphism increased PEX1 promoter activity by 25%. When co-expressed (mimicking the physiological condition) these polymorphisms compensated for each other to bring PEX1 promoter activity to near wild-type levels. The PEX1 mutations identified in this study have been utilised by collaborators at the National Referral Laboratory for Lysosomal, Peroxisomal and Related Genetic Disorders (based at the Women's and Children's Hospital, Adelaide), in prenatal diagnosis of the PBDs. In addition, the identification of three common mutations in Australasian PBD patients has led to the implementation of screening for these mutations in newly referred patients, often enabling a precise diagnosis of a PBD to be made. Finally, the strong correlation between genotype and phenotype for the patient cohort investigated as part of these studies has generated a basis for the assessment of newly identified mutations in PEX1.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
Full Text
APA, Harvard, Vancouver, ISO, and other styles
4

Harper, Courtney Christine. "Complex problems in peroxisome matrix protein import." Available to US Hopkins community, 2003. http://wwwlib.umi.com/dissertations/dlnow/3080674.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Su, Jian-Rong. "Structural studies of the peroxisomal matrix protein import factor, Pex14p." 京都大学 (Kyoto University), 2010. http://hdl.handle.net/2433/120677.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Meshram, Mallika. "PEX13 Mutant Mice as Models of Zellweger Syndrome Neuropathogenesis and Peroxisomal Matrix Protein Import." Thesis, Griffith University, 2014. http://hdl.handle.net/10072/366010.

Full text
Abstract:
Peroxisomes are essential for the developing brain as the loss of functional peroxisomes leads to mild to severe Peroxisome Biogenesis Disorders (PBD) with significant neurological involvement. Zellweger Syndrome (ZS) represents the most severe form of PBDs resulting from a mutation in PEX genes, including PEX13, which encode peroxins necessary for peroxisome biogenesis. ZS patients exhibit a range of clinical abnormalities including hypotonia, multi-organ failure, abnormal metabolic profile with significant neuropathologies and death within a year after birth. As mutation in Pex13 in humans result in ZS, animal models with ubiquitous and targeted disruption of PEX13 were generated in order to understand the unifying molecular pathogenesis of ZS. PEX13 knockout (KO) and brain specific disruption of PEX13 mice (referred as PEX13 brain mutant) were developed in our laboratory and applied as experimental tools previously and in this thesis, to explore the molecular and cellular basis of ZS neuropathology. The PEX13 KO mice, exhibits severe ZS phenotype which includes hypotonia, neuronal migration defect, impaired fatty acid oxidation and plasmalogen synthesis, and early neonatal death.
Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Physical Sciences
Science, Environment, Engineering and Technology
Full Text
APA, Harvard, Vancouver, ISO, and other styles
7

Reglinski, Katharina [Verfasser], Ralf [Akademischer Betreuer] Erdmann, and Matthias [Akademischer Betreuer] Rögner. "Bedeutung und Mechanismen des peroxisomalen Matrix‐Protein‐Importes des deubiquitinierenden Enzyms USP2 / Katharina Reglinski. Gutachter: Ralf Erdmann ; Matthias Rögner." Bochum : Ruhr-Universität Bochum, 2016. http://d-nb.info/1095884859/34.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Dias, Ana Filipa Carvalho Marques. "The peroxisomal matrix protein import machinery - a PEX5 - centered perspective." Doctoral thesis, 2017. https://repositorio-aberto.up.pt/handle/10216/107578.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Dias, Ana Filipa Carvalho Marques. "The peroxisomal matrix protein import machinery - a PEX5 - centered perspective." Tese, 2017. https://repositorio-aberto.up.pt/handle/10216/107578.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Yen, Yu-Chen, and 嚴玉真. "The assessment of the importence of the nearby sequence before peroxisomal targeting sequence (PTS1) on the transport of peroxisomal matrix protein SPS19 in Saccharomyces cerevisiae." Thesis, 2013. http://ndltd.ncl.edu.tw/handle/49810494097136559460.

Full text
Abstract:
碩士
中山醫學大學
生化暨生物科技研究所
101
Peroxisomal matrix proteins are imported from cytoplasm into peroxisomes through peroxisomal targeting signal 1 (PTS1). Peroxisomal targeting signal (PTS) type 1 (PTS1) is a C-terminal uncleaved tripeptide (serine-lysine-leucine [SKL] or variants). Analysis of sequence variability in the PTS1 motif revealed that, in addition to the known C-terminal tripeptide, at least nine residues directly upstream are important for signal recognition in the PTS1:Pex5p receptor complex. The yeast SPS19 gene encoding the peroxisomally targeted 2,4-dienoyl-CoA reductase ，fatty acids with double bonds at even-numbered positions require for their degradation the auxiliary enzyme 2,4-dienoyl-CoA reductase。The Saccharomyces cerevisiae SPS19 deleted was reduction in sporulation and sensitive to lytic.SPS19 reveled a carboxyl-terminal SKL peroxisome targeting signal. In this study , we constructed pRS424-Gal-GFP-PTS1-12a，pRS424-Gal-GFP-PTS1-3ａ, and pRS424-Gal-GFP, transformed these plasmid into wild-type W303.1a and W303.1a-Δpex19 by yeast transformation technique .The GFP-PTS1 encoding these strains were assayed peroxisome formation by the fluorescence microscopy technique.pRS424-Gal-GFP-PTS1-12a and pRS424-Gal-GFP-PTS1-3a DNA sequence use ClustalW2 analysis to perform comparative sequencing .We find the expected results for the pRS424-Gal-GFP-PTS1-20a and pRS424-Gal-GFP-PTS1-12a have green fluorescence in peroxisome, but the pRS424-Gal-GFP-PTS1-3a has green fluorescence less than the pRS424-Gal-GFP-PTS1-20a and pRS424-Gal-GFP-PTS1-12a in peroxisome, and pRS424-Gal-GFP have green fluorescence in cytosol. These results suggest that the C-terminal 12 amino acids of SPS19 is better sequence reguired for effective protein transport into peroxisome.
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Peroxisomal matrix proteins"

1

Reumann, Sigrun, and Gopal Chowdhary. "Prediction of Peroxisomal Matrix Proteins in Plants." In Proteomics of Peroxisomes, 125–38. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2233-4_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Freitag, Johannes, Thorsten Stehlik, Alina C. Stiebler, and Michael Bölker. "The Obvious and the Hidden: Prediction and Function of Fungal Peroxisomal Matrix Proteins." In Proteomics of Peroxisomes, 139–55. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2233-4_6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Mullen, Robert T. "Targeting and Import of Matrix Proteins into Peroxisomes." In Plant Peroxisomes, 339–83. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/978-94-015-9858-3_11.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Effelsberg, Daniel, Ralf Erdmann, and Wolfgang Schliebs. "The Matrix Protein Import Complex in Yeast." In Molecular Machines Involved in Peroxisome Biogenesis and Maintenance, 305–23. Vienna: Springer Vienna, 2014. http://dx.doi.org/10.1007/978-3-7091-1788-0_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Okumoto, Kanji, Masanori Honsho, Yuqiong Liu, and Yukio Fujiki. "Peroxisomal Membrane and Matrix Protein Import Using a Semi-Intact Mammalian Cell System." In Methods in Molecular Biology, 213–19. New York, NY: Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6937-1_20.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Leon, Sebastien, and Suresh Subramani. "The Role of Shuttling Targeting Signal Receptors and Heat‐Shock Proteins in Peroxisomal Matrix Protein Import." In Molecular Machines Involved in Protein Transport across Cellular Membranes, 525–40. Elsevier, 2007. http://dx.doi.org/10.1016/s1874-6047(07)25020-6.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Platta, Harald W., Sven Thoms, Wolf‐H Kunau, and Ralf Erdmann. "Function of the Ubiquitin‐Conjugating Enzyme Pex4p and the AAA Peroxin Complex Pex1p/Pex6p in Peroxisomal Matrix Protein Transport." In Molecular Machines Involved in Protein Transport across Cellular Membranes, 541–72. Elsevier, 2007. http://dx.doi.org/10.1016/s1874-6047(07)25021-8.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "Peroxisomal matrix proteins"

1

Fluhr, Robert, and Maor Bar-Peled. Novel Lectin Controls Wound-responses in Arabidopsis. United States Department of Agriculture, January 2012. http://dx.doi.org/10.32747/2012.7697123.bard.

Full text
Abstract:
Innate immune responses in animals and plants involve receptors that recognize microbe-associated molecules. In plants, one set of this defense system is characterized by large families of TIR–nucleotide binding site–leucine-rich repeat (TIR-NBS-LRR) resistance genes. The direct interaction between plant proteins harboring the TIR domain with proteins that transmit and facilitate a signaling pathway has yet to be shown. The Arabidopsis genome encodes TIR-domain containing genes that lack NBS and LRR whose functions are unknown. Here we investigated the functional role of such protein, TLW1 (TIR LECTIN WOUNDRESPONSIVE1). The TLW1 gene encodes a protein with two domains: a TIR domain linked to a lectin-containing domain. Our specific aim in this proposal was to examine the ramifications of the TL1-glycan interaction by; A) The functional characterization of TL1 activity in the context of plant wound response and B) Examine the hypothesis that wounding induced specific polysaccharides and examine them as candidates for TL-1 interactive glycan compounds. The Weizmann group showed TLW1 transcripts are rapidly induced by wounding in a JA-independent pathway and T-DNA-tagged tlw1 mutants that lack TLW1 transcripts, fail to initiate the full systemic wound response. Transcriptome methodology analysis was set up and transcriptome analyses indicates a two-fold reduced level of JA-responsive but not JA-independent transcripts. The TIR domain of TLW1 was found to interact directly with the KAT2/PED1 gene product responsible for the final b-oxidation steps in peroxisomal-basedJA biosynthesis. To identify potential binding target(s) of TL1 in plant wound response, the CCRC group first expressed recombinant TL1 in bacterial cells and optimized conditions for the protein expression. TL1 was most highly expressed in ArcticExpress cell line. Different types of extraction buffers and extraction methods were used to prepare plant extracts for TL1 binding assay. Optimized condition for glycan labeling was determined, and 2-aminobenzamide was used to label plant extracts. Sensitivity of MALDI and LC-MS using standard glycans. THAP (2,4,6- Trihydroxyacetophenone) showed minimal background peaks at positive mode of MALDI, however, it was insensitive with a minimum detection level of 100 ng. Using LC-MS, sensitivity was highly increased enough to detect 30 pmol concentration. However, patterns of total glycans displayed no significant difference between different extraction conditions when samples were separated with Dionex ICS-2000 ion chromatography system. Transgenic plants over-expressing lectin domains were generated to obtain active lectin domain in plant cells. Insertion of the overexpression construct into the plant genome was confirmed by antibiotic selection and genomic DNA PCR. However, RT-PCR analysis was not able to detect increased level of the transcripts. Binding ability of azelaic acid to recombinant TL1. Azelaic acid was detected in GST-TL1 elution fraction, however, DHB matrix has the same mass in background signals, which needs to be further tested on other matrices. The major findings showed the importance of TLW1 in regulating wound response. The findings demonstrate completely novel and unexpected TIR domain interactions and reveal a control nexus and mechanism that contributes to the propagation of wound responses in Arabidopsis. The implications are to our understanding of the function of TIR domains and to the notion that early molecular events occur systemically within minutes of a plant sustaining a wound. A WEB site (http://genome.weizmann.ac.il/hormonometer/) was set up that enables scientists to interact with a collated plant hormone database.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Peroxisomal matrix proteins' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography