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1
Simis, André. "Edema peritumoral em meningiomas benignos: correlação com fatores clínicos, radiológicos, cirúrgicos e com recorrência tumoral." Universidade de São Paulo, 2007. http://www.teses.usp.br/teses/disponiveis/5/5138/tde-12022008-132122/.
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INTRODUÇÃO: O edema peritumoral (EP) está presente em aproximadamente 60% dos meningiomas. Os fatores responsáveis pela formação do edema e sua importância clínica permanecem como foco de discussão. OBJETIVOS: Analisar a correlação entre a presença de edema com características clínicas, cirúrgicas, radiológicas e recorrência tumoral. MÉTODOS: Foram selecionados 61 pacientes portadores de meningiomas benignos submetidos a tratamento cirúrgico pelo Grupo de Tumores Encefálicos e Metástases do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo. Foram incluídos no estudo os portadores de meningiomas benignos submetidos a ressecção tumoral completa (Simpson 1 e 2). Foram excluídos pacientes portadores de meningiomas malignos ou atípicos e aqueles localizados em tubérculo selar, seio cavernoso, forame magno, intraventriculares e região petroclival. RESULTADOS: Encontramos correlação entre as maiores medidas de edema peritumoral e recorrência tumoral (p = 0,042) e tumores com margens irregulares (p < 0,011) na análise bivariada. Além disso, os pacientes que apresentaram maiores volumes tumorais apresentaram maiores medidas de edema (p = 0,035) e nos pacientes com menores medidas de edema a localização tentorial foi mais freqüente (p = 0,032). Verificamos que ao estudo de regressão logística, o EP apresenta correlação com tumores maiores que 40 cm3 (Odds ratio=15,977), crises convulsivas (Odds ratio=3,469) e para cada cm3 acrescida ao tamanho tumoral o risco de edema cresce 1,082 vez (Odds ratio). CONCLUSÕES: Considerando os resultados obtidos, o EP esteve associado a maior recorrência tumoral, tumores multilobulados, grandes e a presença de crises convulsivas. A localização tentorial mostrou-se como um fator protetor ao EP. O EP pode estar associado a um potencial invasivo aumentado em meningiomas. Desta forma, o seu estudo aprofundado poderá trazer dados adicionais para o esclarecimento dos mecanismos de formação dos meningiomas e de seu comportamento biológico levando ao melhor manejo clínico dos pacientes.INTRODUCTION: Approximately 60% of meningiomas are associated with peritumoral edema.Various causative factors have been discussed in the literature. PURPOSES: Investigate the correlation of peritumoral edema with clinical, radiological and surgical aspects, and recurrence rate of meningiomas. METHODS: Sixty one benign meningiomas submitted to surgical treatment by the Group of Brain Tumors and Metastasis of the Division of Neurosurgery of the Hospital das Clínicas of São Paulo Medical School of São Paulo University. All patients underwent complete surgical ressection (Simpson 1 and 2) and were excluded the atypical and malignant hystopathological grades. The tumors located in the cavernous sinus, tuberculum sellae region, foramen magnum region, ventricular space and petroclival region were excluded. RESULTS: Edema extention had a positive correlation with the higher recurrence rates (p = 0,042) and with the presence of irregular margins (p < 0,011) on bivariate analysis. Meningiomas with greater edema sizes also showed correlation with large meningiomas (p = 0,035) and the ones with smaller edema sizes correlated with the tentorial location (p=0,032). Multivariate analysis showed an association between peritumoral brain edema and the presence of seizures (Odds ratio=3,469), large meningiomas (Odds ratio=15,977), and for each cubic centimeter added to its size, the risk of edema increased 1,082 times (Odds ratio). CONCLUSION: Peritumoral brain edema correlated with recurrence, irregular margins, seizures and larger tumors. The tentorial location demonstrated smaller edema sizes. Peritumoral brain edema may be related to meningioma\'s invading potentiality and may play a role in the recurrence pontential of the tumor. As a consequence, it\'s reasonable to consider edema\'s presence as an additional factor to be taken into account when arranging layout of strategies for meningiomas treatment.
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Vieira, Fabricio Le Draper. "Tipagem das células do infiltrado inflamatório peritumoral em carcinoma de células escamosas da mucosa bucal: correlação com expressão de Ki67." Universidade do Estado do Rio de Janeiro, 2009. http://www.bdtd.uerj.br/tde_busca/arquivo.php?codArquivo=4554.
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O emprego de técnicas imunoistoquímicas, utilizando marcadores biológicos como o Ki67, que permite a avaliação do índice de proliferação celular em neoplasias malignas, vem sendo preconizado como um importante caminho de investigação do comportamento biológico das neoplasias malignas, tendo como consequências contribuições para o estabelecimento do prognóstico e desenvolvimento de novos protocolos terapêuticos. Neste trabalho, utiliza-se o método imunoistoquímico da avidina-biotina-peroxidase avaliada a expressão de Ki67 no parênquima de amostras de carcinomas de células escamosas da mucosa bucal com diferentes graus de diferenciação histológica. Além disso, a quantificação da área de infiltrado inflamatório foi avaliada. Os resultados demonstraram que a resposta imunológica celular é o principal mecanismo de defesa no carcinoma de células escamosas da mucosa bucal, expressada pelo grande número de linfócitos T e macrófagos e a expressão de Ki67 está relacionado ao índice mitótico e, consequentemente, à proliferação celular e, também, à diferenciação da neoplasia.The utilization of immunohistochemical techniques as biological markers, such as Ki67, that allows the evaluation of the cell proliferation in malignancies, has been advocated as an important way of investigating the biological behavior of cancer, resulting in contributions to the establishment of prognosis and development of new treatment protocols. In this study, using immunohistochemical methods with avidin-biotin-peroxidase, we have assessed the expression of Ki67 in samples of oral squamous cell carcinoma with different patterns of histologic differentiation. The results showed that the cellular immune response is the major deffence mechanisnc in squamous cell carcinoma of the oral mucosa, expressed by the large number of T lymphocytes and macrophages, and expression of Ki67 is related to mitotic index associated with cell proliferation and neoplastic differentiation of tumor.
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Silva, Júlio César Thomé de Souza. "Correlação da localização topográfica e do edema peritumoral em pacientes com glioma recidivo com a resposta terapêutica ao álcool perílico." Niterói, 2010. https://app.uff.br/riuff/handle/1/4846.
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Universidade Federal Fluminense. Hospital Universitário Antonio Pedro
Hospital Federal de Ipanema
Hospital Municipal Souza Aguiar
Introdução: Gliomas são tumores cerebrais primários caracterizados pelo crescimento difuso e invasivo. Estudo biomatemático de proliferação e migração dos gliomas propõe que o crescimento de tumores na substância cinzenta profunda cerebral teria um intervalo de tempo maior comparado a lesões situadas na substância branca lobar, onde a invasão e migração seriam mais rápidas. Objetivo: Estabelecer uma correlação da topografia tumoral e edema peritumoral com a resposta terapêutica à administração intranasal do álcool perílico (AP) em pacientes com glioblastoma (GBM) recidivo após tratamento convencional. Métodos: A coorte incluiu o estudo retrospectivo de 119 pacientes com glioma recidivo, sendo 52 em tratamento paliativo de suporte (grupo controle não pareado) e 67 que foram incluídos no Estudo Fase I/II do álcool perílico e receberam administração pela via inalatória de 440 mg de AP diariamente durante o período de Janeiro 2005 a Dezembro 2009. Os parâmetros avaliados incluíram aspectos clínicos e de neuroimagem: topografia tumoral, volume tumoral, presença de desvio da linha média e extensão de edema peritumoral; análise dos dados demográficos, sintomas iniciais e sobrevida global. Análise estatística foi realizada usando testes log rank. A sobrevida global foi determinada pelo método de Kaplan-Meier, incluindo intervalos de confiança de 95%. Resultados: Pacientes do grupo controle apresentaram sobrevida reduzida (p < 0,0001) em relação ao grupo tratado com AP. Dentre os 67 pacientes com GBM, 14 (21%) apresentavam localização talâmica e 53 (79%) localização lobar. Pacientes com tumor localizado na região do tálamo sobreviveram significativamente mais tempo do que aqueles com tumor localizado na região lobar (log rank test, p = 0,0003). Pacientes que apresentaram regressão tumoral acompanhada de redução do edema peritumoral apresentaram resposta clínica positiva, enquanto aqueles com regressão tumoral sem redução do edema peritumoral apresentaram evolução clínica desfavorável, independentemente da topografia tumoral. Presença de desvio da linha média (> 1 cm) foi estatisticamente significante como fator de risco para menor sobrevida (log rank test, p = 0,0062). Conclusões: Este estudo sugere que: (1) pacientes com tumor localizado na região profunda (tálamo) apresentaram sobrevida média maior do que pacientes com tumor localizado na região lobar; (2) edema peritumoral foi um fator determinante na sintomatologia, provavelmente implicado na morbidade podendo estar relacionado com a característica de invasividade do glioma maligno. Esses achados apóiam a teoria de que fatores presentes em diferentes microambientes do tecido cerebral (tálamo, córtex) possam contribuir para o processo de progressão tumoral, para o prognóstico clínico e a resposta terapêutica ao álcool perílico administrado pela vias inalatória
Introduction: Gliomas are primary brain tumors are characterized by diffuse and invasive growth. Study biomathematical proliferation and migration of gliomas suggests that the growth of tumors in deep brain gray matter would have a longer time interval compared with lesions in the lobar white matter, where the invasion and migration would be faster. Objective: To establish a correlation of peritumoral edema and tumor topography with the therapeutic response to intranasal administration of perillyl alcohol (PA) in patients with glioblastoma (GBM) relapsing after conventional treatment. Methods: The cohort included a retrospective study of 119 patients with relapsing glioma, 52 palliative care support (control group) and 67 that were included in the Study Phase I / II and received perillyl alcohol administration by inhalation of 440 mg AP daily during the period January 2005 to December 2009. The parameters evaluated included clinical and neuroimaging: topography tumor, tumor volume, presence of midline shift and extent of peritumoral edema, analysis of demographic data, initial symptoms and overall survival. Statistical analysis was performed using log rank tests. Overall survival was determined by Kaplan-Meier, including 95% confidence intervals. Glioma is a primary brain tumor characterized by diffuse growth and invasiveness. The pattern of differential tumor growth and invasiveness suggest that patients with tumoral lesion located in the lobar white matter region present lower survival rate than patients with lesion located in deep brain gray matter (thalamo). Objective: To establish a correlation between tumor topography and peritumoral edema with the therapeutic response to intranasal administration of perillyl alcohol (POH). Methods: This retrospective study analyzed 119 patients with recurrent glioma being 52 under supportive treatment (control group) and 67 included in the Phase I/II clinical trial that received intranasal administration of 440 mg daily AP from January 2005 to December 2009. The following parameters were analyzed: clinical assessment; demographic data, symptoms and overall survival, neuroimage analysis of topography including tumor volume, midline shift and extent of peritumoral edema. Statistical analysis was performed using log rank tests. The overall survival was determined by the Kaplan-Meier method, including 95% confidence intervals. Results: Patients from control group showed reduced overall survival (p < 0,0001) in comparison with patients included in the Phase I/II that received treatment with perillyl alcohol. Among 67 GBM patients, 14 (21%) had tumoral lesion in the thalamic region and 53 (79%) in the lobar region. Patients with thalamic tumor survived significantly longer than those with tumor located in the lobar region (log rank test, p = 0.0003). Patients with tumor regression with reduction of peritumoral edema had positive clinical response, whereas poor prognosis was observed in those with tumor regression but without reduction of peritumoral edema. Presence of midline shift (> 1 cm) was statistically significant as a risk factor for shorter survival (log rank test, p = 0062). Conclusions: This study indicates that: 1) patients with tumoral lesion in the deep region (thalamic) have longer overall survival than GBM patients with tumors in the lobar region; 2) presence of peritumoral edema contributes strongly to symptoms and is likely to influence morbidity and the invading potential of malignant glioma. These findings support the hypothesis that interaction between glioma cells and different brain microenvironment (thalamo, cortex) can influence the process of glioma progression, clinical prognosis and therapeutic response to intranasal delivery perillyl alcohol
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Grochot, Rafael Maciel. "Expressão do PD-L1 em neoplasias cervicais e seu impacto em sobrevida associado à infiltração linfocitária peritumoral e à expressão de FOXP3." reponame:Repositório Institucional da UCS, 2018. https://repositorio.ucs.br/11338/3902.
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Mendes, Suzanny Oliveira. "Perfil da expressão de HIF-1α em células linfoides do infiltrado inflamatório peritumoral e intratumoral como marcador prognóstico do carcinoma epidermoide de cavidade oral." reponame:Repositório Institucional da UFES, 2014. http://repositorio.ufes.br/handle/10/1887.
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O complexo transcricional HIF-1 é responsável por controlar a transcrição de mais de 100 genes envolvidos em resposta celular a hipóxia. A subunidade HIF-1α é estabilizada em condições de hipóxia e dimeriza-se com um a subunidade HIF-1β, formando o complexo HIF-1 transcricionalmente ativo. Em células inflamatórias, uma elevada expressão de HIF-1α pode induzir os linfócitos à imunossupressão, reduzindo o reconhecimento dos antígenos tumorais, permitindo o crescimento tumoral. O presente trabalho objetivou investigar a relação entre a expressão de HIF-1α em linfócitos do infiltrado inflamatório intratumoral e peritumoral de 56 pacientes com carcinoma epidermoide de cavidade oral. Os resultados indicaram um valor prognóstico para esta expressão. Uma elevada expressão da HIF-1α em células do infiltrado inflamatório peritumoral foi significantemente relacionada com o pior prognóstico do paciente, enquanto esta elevada expressão nos linfócitos na região intratumoral foi correlacionado com um melhor prognóstico. Um perfil de risco indicando a chance para a recidiva e óbito foi desenhado baseado na expressão de HIF-1α nos linfócitos do infiltrado inflamatório peritumoral e intratumoral, definindo os riscos baixo, intermediário e alto. Este perfil de risco foi hábil para determinar que a expressão forte de HIF-1α nos linfócitos peritumorais são relacionados com um pior prognóstico, independente da expressão nas células intratumorais. A expressão fraca de HIF-1α nos linfócitos peritumorais e forte nos linfócitos intratumorais foram considerados um perfil de baixo risco, mostrando nenhum caso de recidiva da doença e óbito decorrente da doença. O risco intermediário foi associado com baixa expressão de HIF-1α tanto em infiltrado inflamatório intratumoral como peritumoral. Estes resultados sugerem que a expressão da HIF-1α em células linfoides do infiltrado inflamatório intratumoral e peritumoral pode exercer um papel importante como um marcador prognóstico tumoral.
The HIF-1 transcriptional complex is responsible for controlling transcription of over 100 genes involved in cell hypoxia response. HIF-1α subunit is stabilized in hypoxia conditions, dimerizes with HIF-1β forming the active HIF-1 transcriptional factor. In inflammatory cells, high HIF-1α expression might induce lymphocytic immunosuppression, decreasing tumoral antigen recognition, allowing tumor growth. The present work aimed to investigate the relationship between HIF-1α expression in lymphocytes inflammatory infiltrate from intratumoral and peritumoral region of 56 patients with oral cancer. Data indicates a prognostic value for this expression. High HIF-1α expression in peritumoral inflammatory cells was significantly related to worse patient outcome, whereas high expression in the intratumoral lymphoid cells correlates with a better prognosis. A risk profile indicating the chance of disease relapse and death was designed based on HIF-1α expression in inflammatory infiltrate cells, defining low, intermediate and high risks. This risk profile was able to determine that high HIF-1α expression in peritumoral lymphocytes correlates with worse prognosis, independently of intratumoral inflammatory infiltrate expression. Low HIF-1α in peritumoral lymphocytes and high expression in the intratumoral lymphocytes was considered a low risk profile, showing no cases of disease relapse and disease related death. Intermediate risk was associated with low HIF-1α expression in inflammatory infiltrate intratumoral and peritumoral. in tumor and tumor margins. These results suggest that HIF-1α expression in tumor and peritumoral inflammatory cells may play an important role as prognostic tumor marker.
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MENDES, S. O. "Perfil da Expressão de Hif-1 Alfa em Células Linfoides do Infiltrado Inflamatório Peritumoral e Intratumoral Como Marcador Prognóstico do Carcinoma Epidermoide de Cavidade Oral." Universidade Federal do Espírito Santo, 2014. http://repositorio.ufes.br/handle/10/4481.
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Previous issue date: 2014-02-21O complexo transcricional HIF-1 é responsável por controlar a transcrição de mais de 100 genes envolvidos em resposta celular a hipóxia. A subunidade HIF-1α é estabilizada em condições de hipóxia e dimeriza-se com um a subunidade HIF-1β, formando o complexo HIF-1 transcricionalmente ativo. Em células inflamatórias, uma elevada expressão de HIF-1α pode induzir os linfócitos à imunossupressão, reduzindo o reconhecimento dos antígenos tumorais, permitindo o crescimento tumoral. O presente trabalho objetivou investigar a relação entre a expressão de HIF-1α em linfócitos do infiltrado inflamatório intratumoral e peritumoral de 56 pacientes com carcinoma epidermoide de cavidade oral. Os resultados indicaram um valor prognóstico para esta expressão. Uma elevada expressão da HIF-1α em células do infiltrado inflamatório peritumoral foi significantemente relacionada com o pior prognóstico do paciente, enquanto esta elevada expressão nos linfócitos na região intratumoral foi correlacionado com um melhor prognóstico. Um perfil de risco indicando a chance para a recidiva e óbito foi desenhado baseado na expressão de HIF-1α nos linfócitos do infiltrado inflamatório peritumoral e intratumoral, definindo os riscos baixo, intermediário e alto. Este perfil de risco foi hábil para determinar que a expressão forte de HIF-1α nos linfócitos peritumorais são relacionados com um pior prognóstico, independente da expressão nas células intratumorais. A expressão fraca de HIF-1α nos linfócitos peritumorais e forte nos linfócitos intratumorais foram considerados um perfil de baixo risco, mostrando nenhum caso de recidiva da doença e óbito decorrente da doença. O risco intermediário foi associado com baixa expressão de HIF-1α tanto em infiltrado inflamatório intratumoral como peritumoral. Estes resultados sugerem que a expressão da HIF-1α em células linfoides do infiltrado inflamatório intratumoral e peritumoral pode exercer um papel importante como um marcador prognóstico tumoral.
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Makdissi, Fabiana Baroni Alves. ""Análise da expressão de E-caderina, Snail e Hakai em células epiteliais de tumor e tecido peritumoral de mulheres com carcinoma ductal invasivo da mama: correlação com comprometimento linfonodal"." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5155/tde-07082006-110753/.
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A expressão de E-caderina (Ecad) pode ser regulada pré ou pós transcricionalmente por Snail e Hakai, respectivamente. Nosso objetivo foi determinar a expressão de Ecad, Snail e Hakai, em células epiteliais (CE) de tecido tumoral e peritumoral de pacientes com carcinoma ductal invasivo da mama e correlacionar sua expressão ao comprometimento linfonodal axilar (LN+). As CE de amostras de tecidos de 45 pacientes (52% LN+) foram extraídas por método imunomagnético, o RNA foi extraído por RT-PCR em tempo real e utilizou-se primers específicos para a moléculas. A expressão de Ecad, Snail e Hakai não variou entre o tecido tumoral e peritumoral e não houve correlação com comprometimento linfonodalE-cadherin (Ecad) expression may be transcriptionally or post-transcriptionally regulated by Snail and Hakai. Our aim was to determine the expression of Ecad, Snail and Hakai, in epithelial cells (EC) obtained from tumor and its adjacent tissue from women with invasive ductal breast carcinoma (IDC) and evaluate their correlation to the axillary's lymph node (LN+) involvement. Tissue from 45 patients (52% LN+) had their EC recovered by immunomagnetic antibody process, RNA was extracted and real-time RT-PCR was performed using specific primers. Ecad, Snail and Hakai mRNA expression did not vary between tumoral and peritumoral samples and their expression was not correlated to LN involvement
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GIANNICE, RAFFAELLA. "Il microambiente peritumorale nel carcinoma dell'endometrio." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2012. http://hdl.handle.net/10281/31294.
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INTRODUZIONE Sempre maggiori evidenze scientifiche confermano che il microambiente peri tumorale gioca un ruolo importante nello sviluppo e nel comportamento dei tumori solidi.
Nelle ultime decadi la correlazione tra tumore e risposta infiammatoria peritumorale è stata ampiamente accettata indicando il ruolo centrale del sistema immunitario nella progressione tumorale e nella prognosi. Obiettivo primario dello studio è stato analizzare l’espressione dell’mRNA di alcune citochine, chemochine e loro recettori e di alcuni fattori di crescita, estratti da campioni di carcinoma originato da endometrio umano e confrontarli con l’ mRNA estratto da campioni di tessuto endometriale normale omologo al fine di caratterizzare le variazioni del microambiente tumorale del carcinoma endometriale rispetto al tessuto endometriale sano.
L’obiettivo secondario è stato evidenziare i fattori più interessanti presenti nell’ambiente peritumorale del carcinoma endometriale per programmare uno studio prospettico con una casistica maggiore.
MATERIALE E METODO. Presso l’istituto Clinico di Ricerca a Carattere Scientifico IRCCS Humanitas di Rozzano, da pazienti sottoposte a chirurgia primaria per carcinoma dell’endometrio, sono stati prelevati: un campione di carcinoma endometriale (A) e un campione da tessuto endometriale sano della stessa paziente (B) trattati con RNA later e conservati a –80°C. I parametri clinici e chirurgici sono stati raccolti. L’RNA è stato estratto, quantificato, retrotrascritto in cDNA ed infine quantificato mediante una RQ-PCR.
RISULTATI. Dodici pazienti con carcinoma endometriale sono state arruolate nello studio. Nel tessuto tumorale endometriale umano, rispetto al tessuto di controllo sano, sono state osservate i risultati statisticamente significativi che seguono. Un’inibizione del CXCL12 mRNA nelle pazienti con infiltrazione del miometrio > 50% (P= .003). Il CXCL12 era direttamente correlato a quello del CXCR7 nel 100% dei casi (P=0.000). L’mRNA del TNF è risultato down-regolato nel 67% dei casi, ed in particolare, nel 100% delle pazienti in cui l’invasione miometriale era > 50% versus il 50% delle pazienti con infiltrazione del miometrio < 50% (P<.09) . Un basso livello di espressione dell’mRNA dell’ IL6 è stato evidenziato nel 100% degli stadi avanzati versus il 45% degli stadi iniziali nel tessuto tumorale rispetto al tessuto sano, (P< .05). Il MIF mRNA aveva un’ aumentata espressione nel 100% dei casi, (P<.001). L’up-regolazione del MIF mRNA era indirettamente proporzionale a quella del TGFb (P<.05).
CONCLUSIONI. In base ai risultati di questo studio, tutti i fattori dell’ambiente peritumorale del carcinoma endometriale esaminati hanno dimostrato livelli di espressione interessanti e meritevoli di approfondimento. Tutti potrebbero essere presi in considerazione quali nuovi target di terapia antitumorale per prolungare l’intervallo libero da malattia o addirittura portare alla guarigione, mediante l’utilizzo di farmaci o anticorpi bloccanti soprattutto nei pazienti a rischio medio-alto ma con stadio del tumore ancora precoce. Il dosaggio dei fattori di crescita, citochine e chemochine esaminati nel nostro studio potrebbe essere utilizzato anchecome fattore prognostico per il tipo di terapia adiuvante, soprattutto negli stadi iniziali. Questi risultati, anche se devono essere confermati in una popolazione piu’ ampia e con un FU maggiore, forniscono un evidenza scientifica che individua nuovi obiettivi per la terapia anti-tumorale per il futuro.
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Lemée, Jean-Michel. "Au delà des frontières du glioblastome : caractérisation de la zone péritumorale des glioblastomes." Thesis, Angers, 2015. http://www.theses.fr/2015ANGE0001/document.
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Le glioblastome (GB) est une tumeur hétérogène, agressive devant laquelle les possibilités thérapeutiques disponibles restent limitées. L’étude de la zone péritumorale macroscopiquement normale (ZMN) des GB est essentielle à la compréhension de ses mécanismes de progression et de récidive. Le premier objectif de ce travail de Thèse a été de comparer les données de transcriptomique et de protéomique issues de l’analyse de la zone tumorale des GB dans le cadre du Projet Gliome Grand Ouest. Le taux de concordance entre les 2 modalités est faible, retrouvant toutefois comme point commun une dysrégulation de la protéine légère des neurofilaments qui pourrait servir de biomarqueur potentiel des GB. Le deuxième objectif de ce travail de Thèse a été la caractérisation de la ZMN des GB. Nous avons mis en évidence que cette zone, dont l’aspect est similaire à première vue à celui du tissu cérébral sain, n’est pas une simple zone de transition entre le GB et le tissu cérébral sain. En effet, la ZMN est une entité spécifique possédant des caractéristiques qui lui sont propres, comme la présence d’un phénotype particulier de cellules tumorales infiltrantes et de cellules stromales et une sur’expression des protéines CRYAB et H3F3A. Ce travail de Thèse a aussi été l’occasion de développer de nouvelles techniques d’imagerie per-opératoire de la ZMN, afin d’évaluer la présence d’un contingent tumoral et ainsi optimiser la qualité de la résection chirurgicale. La caractérisation de cette ZMN nous permet de mieux appréhender son implication dans la tumorogenèse et la présence de caractéristiques spécifiques de cette zone ouvre la porte à la détection de biomarqueurs spécifiques, ainsi qu’au développement de thérapies ciblées. Ce travail de Thèse a été valorisé par 2 publications, 2 articles soumis et un brevet est en cours de dépôt et d’évaluation par un cabinet de brevetGlioblastoma (GB) is a heterogeneous andaggressive tumor, before which therapeutic options arelimited. The study of the macroscopically normalperitumoral brain zone (PBZ) of GB is essential tounderstand its mechanisms of progression andrecurrence.The first objective of this thesis work was tocompare the transcriptomic and proteomic data from theGB tumor area obtained through the “Grand Ouest”glioma Project. The concordance rate between the 2modalities is low. However, one of the common featureis the dysregulation of neurofilament light polypeptide,which could serve as a biomarker potential of GB.The second objective of this thesis was thecharacterization of the PBZ. We have shown that thisarea, similar at first glance to that of healthy braintissue, is not a simple transition area between the GBand healthy brain tissue but a specific entity withcharacteristics of its own. For example, the ZMNpresents a particular phenotype of infiltrating GB cellsand stromal cells and a surexpression of CRYAB andH3F3A proteins.This thesis work was also an opportunity todevelop new intraoperative imaging techniques of thePBZ, with the aim to assess the presence of a tumoralinfiltration and optimize the quality of the surgicalresection.The characterization of this PBZ allows us tobetter understand its involvement in tumorigenesis andthe presence of specific characteristics of this areaopens the door for the detection of specific biomarkersand the development of targeted therapies.This thesis work was led to 2 publications, 2articles submitted and a patent being evaluated andredacted by a patent office
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Sarraf, Michel. "Evaluation non-invasive des Gliomes par Imagerie Résonance Magnétique : Effets des traitements anti-angiogéniques (Avastin) sur la microvascularisation et la microarchitecture tumorale et péritumorale." Thesis, Université Grenoble Alpes (ComUE), 2019. http://www.theses.fr/2019GREAS040.
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En neuro-oncologie, les effets des traitements anti-angiogéniques utilisés pour inhiber la microvascularisation tumorale ne sont pas bien maîtrisés, comme dans le cas des glioblastomes traités par Avastin. Seulement une partie des patients montre une réponse significative au traitement. L’imagerie conventionnelle anatomique ne permet pas d’évaluer l’efficacité de ce traitement. Le défi est de trouver et valider des nouveaux biomarqueurs capables de prédire la réponse tumorale précocement. L’objectif principal de cette thèse est de développer et de mettre en place un protocole préclinique d’imagerie par résonance magnétique (IRM) multiparamétrique, qui est capable de caractériser et de suivre précocement l’évolution après traitement par l’Avastin : la microvascularisation et des microstructures dans la tumeur et sa région péritumorales. Dans ce but, la quantification du volume sanguin (VS), du Kmodel (i.e coefficient apparent d’accumulation de l’agent de contraste (AC)) et l’évaluation de la microarchitecture par la technique d’IRM de diffusion DTI ont été développées et évaluées comme biomarqueurs.Dans un premier temps, nous avons développé et mis en place la méthode Rapid Steady State T1 (RSST1) pour la quantification du VS et du Kmodel dans la tumeur où l’AC s’extravase. Cette technique a été développée initialement pour quantifier le volume sanguin en l’absence d’extravasation de l’AC. Le développement ainsi effectué durant ma thèse repose sur la modélisation du signal d’extravasation RSST1 par un modèle mathématique qui prend en compte l’échange bi-compartimental et unidirectionnel de l’AC du compartiment vasculaire vers le compartiment extravasculaire. Ce développement a permis de quantifier les paramètres vasculaires dans le cas du gliome C6 chez le rat. Les résultats ont été confirmés par d’autres modalités d’imagerie (Technique stationnaire de susceptibilité magnétique et par histologie).Dans un second temps, nous avons étudié la sensibilité de la méthode RSST1 pour le suivi de la réponse tumorale à traitement antiangiogénique dans des conditions proches de la clinique. Pour cette étude, le modèle du gliome humain U87 MG a été utilisé chez la souris sous traitement par Avastin. La méthode RSST1 s’est avérée reproductible pour la mesure du VS et plus sensible que l’imagerie pondérée T1 (avec injection du Gd-DOTA comme AC, T1w-Gd-DOTA) pour détecter et pour suivre la réponse tumorale à l’Avastin notamment à des stades précoces de la progression tumorale. Nos résultats concernant la mesure du VS ont été corrélés aux mesures effectuées par la microscopie à deux photons.Enfin, dans la dernière partie de cette thèse nous avons étudié la capacité de l’imagerie du tenseur de diffusion (DTI), couplée à l’IRM de type FLAIR (fluid-attenuated inversion recovery) et T1w-Gd-DOTA, pour caractériser les régions tumorales, péritumorales et controlatérales dans le modèle U87MG. La quantification des paramètres DTI nous a permis d’évaluer les changements des microstructures dans la région peritumorale avant et pendant l’invasion cellulaire provoquée par l’Avastin pour différents modes d’administration : intraveineux, intratumoral par convection-enhanced delivery. La délinéation des régions péritumorales a été basée sur les images anatomiques, mais nous avons aussi pris en compte la progression intrinsèque de chaque tumeur. Une différence significative des paramètres DTI a été détectée entre les régions tumorales, péritumorales et controlatérales et une évolution différente de ces paramètres a été constatée selon le mode d’injection de l’AvastinIn neuro-oncology, the effects of anti-angiogenic treatments are not predictable, as observed in glioblastomas treated with Avastin. Only a minority of patients show a significant response to treatment. Conventional imaging modalities are not able to evaluate the efficiency in the early phase of the treatment. Thus, the challenge remains to find and to validate new biomarkers that are able to predict the early response to such therapies.The aim of this work is to develop and implement a preclinical multiparametric magnetic resonance imaging (MRI) protocol for the characterization and follow up of early microvascular and microstructural changes in the tumor and its peritumoral regions after treatment with Avastin. For this purpose, the quantification of the blood volume and Kmodel (an apparent coefficient that is related to the contrast agent (CA) uptake rate), and evaluation of brain microarchitecture by diffusion tensor imaging were developed and evaluated as biomarkers.The Rapid Steady State T1 (RSST1) method was initially developed for blood volume quantification in the absence of CA extravasation. In the first part of this thesis, we have implemented and adapted this MRI technique for the quantification of both blood volume and Kmodel in tumors where the CA extravasates. We developed a mathematical model for the RSST1 signals that accounts for the unidirectional bi-compartmental exchange of CA from the vascular towards the extravascular compartment. This development allows to the quantification of vascular parameters in a rat glioma model (C6). The results were confirmed using another MRI modality, the steady state magnetic susceptibility method, and quantitative histology.In the second part, we studied the sensitivity of the RSST1 method for the follow up of the glioma response to anti-angiogenic treatment under clinical conditions. In this study, the effect of Avastin treatment in a murine orthotopic U87 MG glioma model was analyzed. The RSST1 method demonstrated a high reproducibility in the blood volume quantification and a superior sensitivity in comparison to CA enhanced T1-weighted imaging (T1W-Gd-DOTA) for the detection and follow-up of the tumor response to Avastin, especially in early stages of tumor progression. Blood volume quantification by MRI was correlated to measures obtained by two-photon microscopy.In the last part of this thesis, we have studied the capacity of diffusion tensor imaging (DTI) coupled with FLAIR (fluid-attenuated inversion recovery) MRI and T1w-Gd-DOTA, to characterize tumor, peritumoral, and contralateral regions of the U87MG glioma model. We quantified DTI parameters before and during the invasion of tumor cells induced by Avastin in the peritumoral zone for different administration modes: intravenous and intratumoral via Convention-Enhanced Delivery. Therefore, the delineation of peritumoral regions for each tumor in an early stage was based on anatomical images, that took into account the individual tumor progression at later stages. Significant differences were detected for DTI parameters between the tumor, peritumoral, and contralateral regions and a different evolution of these parameters was noticed according to the Avastin injection mode
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Yan, Jiun-Lin. "Characterising peritumoural progression of glioblastoma using multimodal MRI." Thesis, University of Cambridge, 2017. https://www.repository.cam.ac.uk/handle/1810/267740.
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Glioblastoma is a highly malignant tumor which mostly recurs locally around the resected contrast enhancement. However, it is difficult to identify tumor invasiveness pre-surgically, especially in non-enhancing areas. Thus, the aim of this thesis was to utilize multimodal MR technique to identify and characterize the peritumoral progression zone that eventually leads to tumor progression. Patients with newly diagnosed cerebral glioblastoma were included consecutively from our cohort between 2010 and2014. The presurgical MRI sequences included volumetric T1-weighted with contrast, FLAIR, T2-weighted, diffusion-weighted imaging, diffusion tensor and perfusion MR imaging. Postsurgical and follow-up MRI included structural and ADC images. Image deformation, caused by disease nature and surgical procedure, renders routine coregistration methods inadequate for MRIs comparison between different time points. Therefore, a two-staged non-linear semi-automatic coregistration method was developed from the modification of the linear FLIRT and non-linear FNIRT functions in FMRIB’s Software Library (FSL). Utilising the above mentioned coregistration method, a volumetric study was conducted to analyse the extent of resection based on different MR techniques, including T1 weighted with contrast, FLAIR and DTI measures of isotropy (DTI-p) and anisotropy (DTI-q). The results showed that patients can have a better clinical outcome with a larger resection of the abnormal DTI q areas. Further study of the imaging characteristics of abnormal peritumoural DTI-q areas, using MRS and DCS-MRI, showed a higher Choline/NAA ratio (p = 0.035), especially higher Choline (p = 0.022), in these areas when compared to normal DTI-q areas. This was indicative of tumour activity in the peritumoural abnormal DTI-q areas. The peritumoural progression areas were found to have distinct imaging characteristics. In these progression areas, compared to non-progression areas within a 10 mm border around the contrast enhancing lesion, there was higher signal intensity in FLAIR (p = 0.02), and T1C (p < 0.001), and there were lower intensity in ADC (p = 0.029) and DTI-p (p < 0.001). Further applying radiomics features showed that 35 first order features and 77 second order features were significantly different between progression and non-progression areas. By using supervised convolutional neural network, there was an overall accuracy of 92.4% in the training set (n = 37) and 78.5% in the validation set (n=14). In summary, multimodal MR imaging, particularly diffusion tensor imaging, can demonstrate distinct characteristics in areas of potential progression on preoperative MRI, which can be considered potential targets for treatment. Further application of radiomics and machine learning can be potentially useful when identifying the tumor invasive margin before the surgery.
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TERRACCIANO, ROSSANA. "Enhancing the Biodistribution and Physicochemical Properties of Gold Nanoparticles by Modifying their Surface Characteristics." Doctoral thesis, Politecnico di Torino, 2023. https://hdl.handle.net/11583/2976597.
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LIUZZA, Claudia. "Studio dell'infiltrato linfocitario peritumorale e dei linfociti circolanti in pazienti affetti da melanoma cutaneo." Doctoral thesis, Università degli Studi di Palermo, 2014. http://hdl.handle.net/10447/91265.
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I linfociti T γδ sono un gruppo di effettori “natural killer” del sistema immunitario innato che hanno rappresentato un interessante oggetto di studio nel corso degli ultimi anni.
Il Dipartimento di Discipline Chirurgiche, Oncologiche ed Odontostomatologiche del Policlinico Universitario di Palermo, da anni attivo nei confronti della ricerca scientifica, ha recentemente condotto uno studio mirato alla conoscenza del potenziale effetto immunoterapeutico di questa popolazione linfocitaria verso i melanomi in stadio avanzato. Sulla base della classificazione della American Joint Committee on Cancer (AJCC) i pazienti affetti da melanoma maligno in stadio IV con neoplasia metastatica hanno una prognosi infausta, con una bassa sopravvivenza a 5 anni.
Dati statistici stimano che la sopravvivenza globale sia inferiore al 10%, evidenziando come pochi trattamenti terapeutici siano al momento disponibili e ragionevolmente efficaci.
I diversi schemi terapeutici proposti ed approvati per il trattamento del melanoma metastatico (interleukina 2 ad alte dosi e chemioterapia) hanno mostrato un tasso di risposta globale del 16% e del 7,5%, con bassa risposta completa e sopravvivenza a lungo termine.
Lo studio e l’analisi dell’infiltrato linfocitario peritumorale (TIL) nei pazienti affetti da melanoma maligno e la ricerca di linfociti circolanti nel torrente ematico permetterà di definire il ruolo chiave dei linfociti T γδ, e le potenzialità del trattamento terapeutico del melanoma maligno metastatico.γδ T cells are a group of effectors "natural killer" of the innate immune system, which represented an interesting object of study in recent years. The Department of Surgical, Oncology and Dentistry of the University Hospital of Palermo, active for years in respect of scientific research, has recently conducted a study aimed at understanding the potential effect of this immunotherapeutic lymphocyte population to the advanced-stage melanomas. Based on the classification of the American Joint Committee on Cancer ( AJCC ) patients with stage IV malignant melanoma with metastatic cancer have a poor prognosis, with a low 5-year survival. Statistical data estimate that overall survival is less than 10 %, highlighting how few treatments are currently available and reasonably effective. The different therapeutic schemes proposed and approved for the treatment of metastatic melanoma (high-dose interleukin- 2 and chemotherapy) showed an overall response rate of 16 % and 7.5 %, with a low complete response and long-term survival. The study and analysis of the infiltrated peritumoral lymphocytes ( TIL) in patients with malignant melanoma and the pursuit of lymphocytes circulating in the blood stream will allow you to define the key role of γδ T cells, and the potential of the therapeutic treatment of metastatic malignant melanoma.
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MOLINELLI, ELISA. "Melanoma e tessuto adiposo peritumorale: studio preliminare sul ruolo delle adipocitochine nella caratterizzazione e prognosi di malattia." Doctoral thesis, Università Politecnica delle Marche, 2021. http://hdl.handle.net/11566/291060.
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Nelle ultime decadi, è andato delineandosi il concetto di organo adiposo, conferendo al tessuto adiposo una funzione endocrina attiva espletata mediante la secrezione di molteplici citochine e chemochine. Queste sembrano detenere infatti un ruolo chiave non solo nel mantenimento dell'omeostasi energetica ma anche nella patogenesi di malattie metaboliche ed infiammatorie e nella crescita e progressione di numerose neoplasie tra le quali il melanoma. In questo studio sperimentale preliminare abbiamo analizzato l'espressione, a livello del tessuto adiposo sottocutaneo peritumorale mediante qPCR, delle principali adipocitochine (Tumor Nescrosis Factor alpha (TNF-alpha), Interleukin-6 (IL-6), Plasminogen Activator Inhibitor 1 (PAI1), Leptina (LEP), Insulin-like Growth factor 1 (IGF1), Vascular Endothelial Growth Factor A (VEGF-A), Nicotinamide phosphoribosyltransferase (NAMPT), C-X-C Motif Chemokine Ligand 1 (CXCL1) e C-X-C Motif Chemokine Ligand 8 (CXCL8)) ritenute coinvolte, sulla base dei dati presenti in letteratura, nei processi di cancerogenesi e metastatizzazione. Lo studio è stato condotto prendendo in analisi una popolazione composta da pazienti affetti da melanoma, confrontando i dati ottenuti con l'espressione delle stesse citochine nel tessuto adiposo sottocutaneo in 2 gruppi controllo composti rispettivamente da nevi melanocitari e cisti epidemoidi. Abbiamo correlato i risultati ottenuti con i principali fattori prognostici di malattia per comprendere la loro espressione in relazione alla severità di patologia. Abbiamo osservato un aumento statisticamente significativo dell'espressione di PAI1, NAMPT, LEP e CXCL1 a livello del tessuto peritumorale dei campioni di melanoma rispetto ai gruppi controlli ed una correlazione degli stessi con lo stadio patologico di malattia ed in particolare con lo spessore di Breslow (il fattore prognostico più importante nella stadiazione patologica di melanoma). Il limite principale dello studio è rappresentato dal fatto che la popolazione risulta composta da un numero esiguo di pazienti. Studi su casistiche più ampie saranno necessari per confermare i risultati parziali ottenuti. Nel complesso, i risultati preliminari di questo lavoro evidenziano che la sovraespressione di alcune adipochine e chemochine in particolare PAI1, NAMPT, LEP e CXCL1 non solo a livello della lesione melanomatosa ma anche nel tessuto adiposo peritumorale può rappresentare un evento chiave nella crescita e soprattutto nell’aggressività locale della neoplasia e apre pertanto l'ipotesi di un ruolo oncogenico diretto di queste molecole e del tessuto adiposo sottocutaneo nella tumoregenesi del melanoma.In the last decades, the concept of adipose organ has emerged, giving adipose tissue an active endocrine function carried out through the secretion of multiple cytokines and chemokines having a key role not only in maintaining energy homeostasis but also in the pathogenesis of metabolic and inflammatory diseases and in the growth and progression of numerous neoplasms including melanoma. In this preliminary experimental study, we analyzed the expression in the peritumor subcutaneous adipose tissue by qPCR of the most significant adipocytokines involved in the processes of carcinogenesis and metastasis such as Tumor Nescrosis Factor alpha (TNF-alpha), Interleukin- 6 (IL-6), Plasminogen Activator Inhibitor 1 (PAI1), Leptin (LEP), Insulin-like Growth factor 1 (IGF1), Vascular Endothelial Growth Factor A (VEGF-A), Nicotinamide phosphoribosyltransferase (NAMPT), CXC Motif Chemokine Ligand 1 (CXCL1) and CXC Motif Chemokine Ligand 8 (CXCL8) in a population of melanoma patients by comparing the data obtained with the expression of the same cytokines in the subcutaneous adipose tissue of 2 control groups composed respectively of melanocytic nevi and epidemoid cysts. We correlated the results obtained with the main disease prognostic factors to understand their expression in relation to the severity of the disease. We observed a statistically significant increase in the expression of PAI1, NAMPT, LEP and CXCL1 at the level of the peritumor tissue of the melanoma samples compared to the control groups and a correlation of the same with the pathological stage of the disease and in particular with the Breslow thickness (the most important prognostic factor in the pathological staging of melanoma). The main limitation of the study is represented by the small cohort of patients. Studies on larger case series will be necessary to confirm the partial results obtained. Overall, the preliminary results of this study show that the overexpression of adipokines and chemokines in particular PAI1, NAMPT, LEP and CXCL1 not only at the level of the melanomatous lesion but also in the peritumoral adipose tissue, may represent a key event in growth and especially in the local aggressiveness of the neoplasm and therefore opens the hypothesis of a direct oncogenic role of these molecules and of the subcutaneous adipose tissue in the tumorigenesis of melanoma.
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Knoop, Richard F. [Verfasser], and Volker [Akademischer Betreuer] Fendrich. "Peritumorale Entzündung, chronische Pankreatitis und Chemoresistenz von Gemcitabin bei der Therapie des Pankreaskarzinoms – Evaluation am transgenen Tumormausmodell / Richard F. Knoop. Betreuer: Volker Fendrich." Marburg : Philipps-Universität Marburg, 2014. http://d-nb.info/1058679880/34.
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Steinwandter, Florian [Verfasser], Gerhard [Gutachter] Mall, and Claus Michael [Gutachter] Rödel. "Peritumorales Budding bei kolorektalen Karzinomen : Analyse morphologischer Veränderungen an der Invasionsfront / Florian Steinwandter ; Gutachter: Gerhard Mall, Claus Michael Rödel." Frankfurt am Main : Universitätsbibliothek Johann Christian Senckenberg, 2019. http://d-nb.info/1203206763/34.
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Friedrich, Martina [Verfasser], Veit [Akademischer Betreuer] Rohde, Michael [Gutachter] Knauth, Martin [Gutachter] Sommer, and Rainer [Gutachter] Mausberg. "Peritumorale Hirnödeme bei intrakraniellen Meningeomen: Einfluss von Alter, Geschlecht, Tumorgröße, Tumorlokalisation und histologischem Subtyp / Martina Friedrich ; Gutachter: Michael Knauth, Martin Sommer, Rainer Mausberg ; Betreuer: Veit Rohde." Göttingen : Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2017. http://d-nb.info/1149954434/34.
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Kremer-Maas, Marion [Verfasser], Verena [Akademischer Betreuer] Kirn, and Jürgen [Akademischer Betreuer] Wolf. "Der Einfluss der peritumoralen Lymphgefäßdichte auf das Tumorstadium und Outcome von Patientinnen mit Endometriumkarzinom / Marion Kremer-Maas ; Akademische Betreuer: Verena Kirn, Jürgen Wolf." Köln : Deutsche Zentralbibliothek für Medizin, 2018. http://d-nb.info/1174678496/34.
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Baier, Patricia Maria Gloria. "Prognostische Bedeutung der CEA- und CK-20-Detektion mittels RT-PCR in peritumoralen Lymphknoten von Patienten mit nodal negativem kolorektalen Karzinom im UICC-Stadium I und II." [S.l.] : [s.n.], 2004. http://deposit.ddb.de/cgi-bin/dokserv?idn=972767304.
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Lo, Shih-Ya, and 羅仕雅. "Diffusion Tensor Imaging of Peritumoral Structures." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/75058581597483224347.
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碩士國立陽明大學
生物醫學影像暨放射科學系暨研究所
96
Peritumoral signal abnormality (PSA) of brain tumors contains varieties of tissues which contain vasogenic edema and/or tumor infiltration. In clinical experience, T2 weighted MR images are always used to identify PSA in tumor patients. However, the two types of signal abnormalities can hardly be distinguished effectively up to date. In this study, diffusion tensor imaging (DTI) was applied to differentiate the complex contents of PSA. Instead of distinguishing the two components directly, reversibility of PSA was proposed as a criterion to differentiate the tissue contents, which may facilitate tumoral delineation and therefore improve the therapeutic efficiency. The study aims to observe the reversibility of PSA via pre- surgical and at least one-month post-surgical follow-up MR images. PSA were classified as reversible if they were normalized in the follow-up MR imaging and as irreversible if remained. The lesion-to-brain fractional anisotropy (FA) ratio of ROIs was determined. The results showed that mean FA ratios for reversible group (0.517 ± 0.086) and irreversible group（0.306 ± 0.056）were with significant difference（p < 0.0001）. The reversibility of PSA is predictable by mean FA ratios on DTI. The new model for characterization of peritumoral tissues allows us to refine the delineation of therapeutic targets. It may serve as a biomarker for target delineation and therefore improve therapeutic effects.
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Lin, Li-en, and 林立恩. "Diffusion Tensor Images of Metastatic Brain Tumors:the Impact of Peritumoral Edema on White Matter Indices." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/88209165178862397903.
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碩士國立雲林科技大學
工業工程與管理研究所碩士班
100
In recent year, Magnetic Resonance Imaging (MRI) and Diffusion Tensor Imaging (DTI) has become an essential portrait medical treatment, MRI can make extraordinary portrait performances in brain tumor and adjacency edema on medical treatment, DTI can not only observe diversification between brain tumor and white matter fiber bundles but evaluate relationship between tumor and brain tissue. Sometimes brain tumor may undeveloped in T1-weighted MRI, this study will evaluate diversification between brain tumor and other brain tissue by applying region of interest and combining measure index of magnetic resonance diffusion. This thesis will reconstruct 3D visualization for brain tumor and area surrounding edema; then we conduct image fusion after reconstruction. This study will calculate the distance between area surrounding edema and white matter by applying morphological image processing, and then we sort out region of interest, using fractional anisotropy and mean diffusivity to be measurement indicators. This study of brain magnetic resonance imaging include four metastatic cancer patients and two normal subjects, research area cover edema side around tumor of cancer patients and white matter of normal subjects, we develop region of interest by applying morphological image processing. Each step represents that we start three-dimensional dilation to expanse four pixels, in our experiment results; we can find FA rising and MD declining in region of interest of D4、D8 in cancer patients, FA and MD emerge smooth in white matter area beside edema area. FA is the lowest and MD is the highest in D4 in five steps in brain tumor patients experiment results. It demonstrates that the area around tumor will decline direction and rise average diffusion coefficient. This thesis focus on region of interest developed and compares FA and MD with brain tissue in difference area between cancer patients and normal subjects. In our experiments, we can see that results show significant difference in FA and MD in four areas under 90% confidence levels. The values of FA and MD in side of the brain edema and white matter regions outside the area and edema are significant difference（p-value＜0.1）. The values of FA and MD in side of the brain edema region corresponds to the region and against the side are significant difference（p-value＜0.1）. The values of FA and MD in brain edema side against the side of the white matter and the corresponding region are significant difference（p-value＜0.1）. The values of FA and MD in side with the opposition side of the brain tumor area of common concern are significant difference（p-value＜0.1）.We can find the diversification between tumor and white matter area around tumor; we hope this study can offer some references for medical image.
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Chang, Tzu-Yuan, and 張子媛. "Functional characterization of novel α-ketoamide derivatives as potent inhibitors against cathepsin S induced by acidic peritumoral pH of pancreatic cancer." Thesis, 2014. http://ndltd.ncl.edu.tw/handle/4jj3fg.
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Ting, Yi-Cen, and 丁怡岑. "Microstructural Characterization in the Peritumoral Area of Glioma Patients and in the Corpus Callosum of Normal Subjects Using Neurite Orientation Dispersion and Density Imaging (NODDI)." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/hj8ra4.
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碩士國立陽明大學
腦科學研究所
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Diffusion magnetic resonance imaging (dMRI) is a technique for the non-invasive characterization of the microstructure in biological tissues since it is sensitive for the diffusion processes of hydrogen molecules. dMRI is a promising candidate for in vivo quantification of neurite morphology in white matter. Over the past two decades, conventional dMRI method usually focused on Diffusion Tensor Imaging (DTI). DTI was widely used to assess the organization of tissue in white matter, providing some indices to describe changes in biology. The model of DTI describes the diffusive water molecules relevant to free diffusion or hindered anisotropic diffusion homogeneous within each voxel based on the assumption of Gaussian distribution. However, DTI was obtained at single b-value and lacked of specificity for describing tissues in this assumption. Additionally, several advanced dMRI techniques, especially multi-compartment models, have been proposed with complicated assumption for estimating neuron morphology. Neurite Orientation Dispersion and Density Imaging (NODDI) is a clinically feasible technique for estimating the microstructural complexity in central neuron system imaging, post by Zhang et al. in 2012. NODDI is a multi-compartment tissue model based on dMRI, combining a three-compartment tissue model: restricted compartment for non-Gaussian anisotropic diffusion (referring to the space bounded by the membrane of neurites), hindered compartment for Gaussian anisotropic diffusion (referring to the space around the neurites) and isotropic compartment for Gaussian diffusion (referring to the CSF space) in each voxel. Using three compartments, NODDI map not only axons in the white matter but also dendrites in gray matter in each voxel. Compared to DTI indices, NODDI may provide greater specificity to morphology and pathology, e.g. neurite density and orientation dispersion. The aims of this work are to explore the promising indices of diffusion models in characterizing the microstructural complexity in the peritumoral area of gliomas and to show the clinical feasibility and potential capability of NODDI studies. The first chapter gave an overview of dMRI and explained the models of NODDI as well as DTI (Chapter 1). In the chapters 2~5, we investigated the different preprocessing interference on NODDI and DTI as verified by topography of corpus callosum for optimization (Chapter 2), and then we differentiated different types of gliomas and characterized the infiltration in peritumoral area by NODDI and DTI using the optimized preprocessing method (Chapter 3); furthermore, we in-vivo evaluated the simplified NODDI imaging protocol and constructed the semi-automatic regions of interest (ROI) delineation for peritumoral areas (Chapter 4 and 5). Finally, the last chapter gave a conclusion of this thesis (Chapter 6).
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CHAKIR, Asmaa. "Characterization of cancer - associated adipose tissue." Doctoral thesis, 2017. http://hdl.handle.net/11562/960114.
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A large body of evidences has contributed to establish a strict association between adipose tissue and cancer in the contest of metabolic disorders such as obesity. Epidemiological studies have shown that obese patients have a drastically higher risk to develop cancer. This tumor-promoting role in the context of obesity is thought to rely mainly on the aberrant systemic and paracrine release of pro-inflammatory cytokines by fat cells, which ultimately cooperate to boost cancer cell proliferation and metastatic dissemination. In addition, cancer associated adipocytes (CAAs) may contribute in tumor progression by providing a metabolic support to the aberrant bioenergetics demand of cancer cells. However, it still remains to be established whether a relevant pro-inflammatory effect might be exerted by peritumoral adipose tissue in non-obese patients with gastrointestinal cancer. Gastrointestinal cancers are responsible for more deaths than any other cancer in the body. As for other types of cancer, many studies have revealed the strong relationship between gastrointestinal neoplasms and systemic disorders of adipose tissue, such as obesity and diabetes, but almost nothing is known about the local interaction between gastrointestinal cancer cells and peritumoral adipocytes in non-obese/non-diabetic patients. In order to start bridge this gap we assess whether adipose tissue surrounding human gastrointestinal tumors might be altered in terms of adipocyte morphology and inflammatory infiltration. Specifically we compare peritumoral and non-peritumoral adipose tissue (visceral fat distant from tumor lesion) for the adipocyte size and morphology, cell count of lymphocytes (CD3 positive cells, CD3+) and macrophages (CD68 positive cells, CD68+). We found that peritumoral adipose tissue exhibit significantly reduced adipocyte size and increased number of activated lymphocytes and macrophages. Our results provide clinical evidences in support of the emerging notion that adipocytes at the tumor-stroma interface participate in a highly complex vicious cycle organized by cancer cells to promote tumor progression. Specifically our results suggest that peritumoral adipocytes might provide a significant contribution to enhance tumor burden by fueling cancer cell's metabolic demands and providing mitogenic signals via the paracrine release of pro-inflammatory cytokines.
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""Análise da expressão de E-caderina, Snail e Hakai em células epiteliais de tumor e tecido peritumoral de mulheres com carcinoma ductal invasivo da mama: correlação com comprometimento linfonodal"." Tese, Biblioteca Digital de Teses e Dissertações da USP, 2006. http://www.teses.usp.br/teses/disponiveis/5/5155/tde-07082006-110753/.
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Salvador, Rute Maria Silva. "Utilização de imunohistoquímica para avaliação da densidade linfática no mesentério de adenocarcinomas do cólon." Master's thesis, 2019. http://hdl.handle.net/10400.1/13582.
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Introdução: O cancro colorretal apresenta-se como uma das neoplasias mais comuns nos países desenvolvidos. A sua capacidade de metastização ocorre por recurso a vasos sanguíneos e/ou linfáticos. Nas últimas décadas a importância da linfangiogénese no cancro tem sido discutida sendo que, desde que a técnica cirúrgica para a excisão total do mesorreto (ETM) demonstrou efeitos positivos nas recidivas locais do cancro do reto e, mais tarde, com a proposta da implementação da técnica cirúrgica da excisão completa do mesocólon (CME) para o cancro do cólon, a importância da manutenção da integridade do mesocólon e consequente rede linfática tem-se mantido em acesso debate. Até ao momento ainda não se conseguiu entender o verdadeiro benefício da manutenção da integridade da rede linfática e, como tal, a implementação rotineira da CME ainda não ocorreu. Objetivo: Determinar, em doentes com adenocarcinoma do cólon, a densidade linfática mesentérica em regiões peritumorais e em regiões distais ao tumor e correlacionar a densidade linfática mesentérica com diferentes características clínico-patológicas e clínico-histológicas. Métodos: Participaram neste estudo 49 doentes com adenocarcinoma do cólon de estadios T2 e T3. A avaliação da densidade linfática mesentérica foi realizada por imunohistoquímica com recurso ao anticorpo monoclonal D2-40 da podoplanina. Avaliou-se ainda a influência que diversas características clínico-patológicas e clínico-histológicas poderiam ter na densidade linfática mesentérica com recurso ao programa estatístico IBM SPSS. Resultados: Verificou-se maiores densidades linfáticas na região peritumoral comparativamente às regiões distais ao tumor. A densidade linfática peritumoral não se relacionou positivamente com nenhuma das características clínico-patológicas e clínico-histológicas analisadas, no entanto, a densidade linfática distal ao tumor demonstrou significância estatística na relação com o índice de massa corporal, o número de gânglios positivos isolados, a profundidade de invasão do tumor e o envolvimento ganglionar. Conclusão: O papel biológico dos vasos linfáticos na progressão tumoral continua controverso. Apenas se conseguiu correlacionar a densidade linfática com parâmetros clínico-patológicos e clínico-histológicos em regiões distais ao tumor o que reflete a importância de uma técnica cirúrgica bem executada. Continuam a ser necessários mais estudos para a compreensão do papel da linfangiogénese na disseminação tumoral.Introduction: Colorectal cancer presents itself as one of the most common neoplasms in developed countries. Their ability to metastization occurs by resource to blood vessels and/or lymphatic vessels. In recent decades the importance of lymphangiogenesis in cancer has been discussed being that as long as the surgical technique for total mesorectal excision (TME) demonstrated positive effects on local recurrences of rectal cancer and, later, with the proposal for the implementation of complete mesocolic excision (CME) surgical technique for colon cancer, the importance of maintaining the integrity of the mesocolon and consequent lymphatic network it is been keeping himself in intense debate. Until the moment has not yet been understood the true benefit of maintaining the integrity of the lymphatic network and, as such, the routine implementation of the CME has not yet occurred. Objective: Determine, in patients with colon adenocarcinoma, the mesenteric lymphatic density in peritumoral regions and in distal regions to the tumor and correlate the lymphatic density mesenteric with different clinic-pathological and clinic-histological features. Methods: Participated in this study 49 patients with adenocarcinoma of the colon with stages T2 and T3. The evaluation of mesenteric lymphatic density was carried out by immunohistochemistry using the antibody monoclonal D2-40 of podoplanin. It was also evaluated the influence that several clinic-pathological and clinic-histological features could have in mesenteric lymphatic density using the IBM SPSS statistical program. Results: There were higher lymphatic densities in the peritumoral region compared to the distal regions of the tumor. The lymphatic peritumoral density was not positively related to any of the clinic-pathological and clinic-histological characteristics analyzed, however, the lymphatic density distal to the tumor demonstrated statistical significance in the relationship with the body mass index, the number of isolated positive lymph node, the depth of invasion of the tumor and the lymph node involvement. Conclusion: The biological role of the lymph vessels in the tumor progression remains controversial. Only the lymphatic density was correlated with the clinic-pathological and clinic-histological parameters in distal regions to the tumor which reflects the importance of a well-executed surgical technique. Further studies are still needed to understand the role of the lymphangiogenesis in the tumor dissemination.
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Berendes, Katja. "Wertigkeit intra- und peritumoraler Zysten bei Meningeomen im Erwachsenenalter /." 2005. http://bvbr.bib-bvb.de:8991/F?func=service&doc_library=BVB01&doc_number=014588152&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA.
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Friedrich, Martina. "Peritumorale Hirnödeme bei intrakraniellen Meningeomen: Einfluss von Alter, Geschlecht, Tumorgröße, Tumorlokalisation und histologischem Subtyp." Doctoral thesis, 2017. http://hdl.handle.net/11858/00-1735-0000-0023-3F8F-7.
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Rathert, Julian [Verfasser]. "Lesion based fibertracking : ein noninvasiver Traktographie-Algorithmus zur verbesserten Darstellung peritumoraler Faserbahnen in der funktionellen Neuronavigation / vorgelegt von Julian Rathert." 2010. http://d-nb.info/1007160462/34.
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Baier, Patricia Maria Gloria [Verfasser]. "Prognostische Bedeutung der CEA- und CK-20-Detektion mittels RT-PCR in peritumoralen Lymphknoten von Patienten mit nodal negativem kolorektalen Karzinom im UICC-Stadium I und II / Patricia Maria Gloria Baier." 2004. http://d-nb.info/972767304/34.
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