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Journal articles on the topic "PDIA6"
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Passam, Freda H., Angelina Lay, Alexander Dupuy, Jessica Tieng, Lejla Hagimola, Jessica Maclean, Marc Ellis, and Philip Hogg. "Protein Disulphide Isomerase 6 (PDIA6) Attenuates Platelet Endoplasmic Reticulum Stress and Secretion in a Mouse Model." Blood 138, Supplement 1 (November 5, 2021): 3138. http://dx.doi.org/10.1182/blood-2021-152307.
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Abstract Background: Platelet hyperreactivity involves increased secretion of their granule content which promotes platelet aggregation and thrombosis. Platelet hyperreactivity is observed in conditions such as diabetes mellitus and is associated with decreased cardioprotective effect from antiplatelet agents in this patient group. Diabetes is associated with increased endoplasmic reticulum (ER) stress from hyperglycemia and hyperlipidemia. Protein disulphide isomerase 6 (PDIA6) is an endoplasmic reticulum protein which folds nascent proteins by reduction and oxidation of their disulphide bonds. PDIA6 has been shown to inhibit downstream ER stress pathways by inhibiting the phosphorylation of IRE-1 in fibroblasts (Eletto, Mol Cell, 2014). We hypothesized that ER stress pathways are functional in platelets and that PDIA6 may inhibit ER stress pathways leading to platelet secretion. Methods: We generated conditional PDIA6 knockout mice (PF4Cre+ Pdia6 fl/fl) (CKO) in the megakaryocyte/platelet lineage by CRISPR-Cas9 technology (Fig.1A). Megakaryopoiesis and haemostasis was assessed by bone marrow histology, coagulation assays, platelet aggregation and tail bleeding studies. We induced ER stress of purified platelets by incubation with thapsigargin and tunicamycin. Activation of the PERK and IRE1 pathways was measured by Western blot. Thrombosis was assessed in vitro by microfluidic devices and in vivo by electrolytic injury of the carotid artery. Results: PDIA6 CKO mice displayed a mild macrothrombocytopenia: the mean (+/-SD) platelet count in Pf4Cre+/Pdia6fl/fl was 775 +/- 98 x10 3/ul compared with 874 +/- 55 x10 3/ul in Pdia6fl/fl (p<0.005). The median platelet volume was 6.3 fL in Pf4Cre+/Pdia6fl/fl compared with 5.7 fL in Pdia6fl/fl (p<0.005). Megakaryopoiesis was normal at baseline. However, PDIA6 CKO mice showed significant upregulation of intracellular platelet PDIs including PDIA1, PDIA3 and PDIA4. PDIA6 deficient platelets displayed significant increase of disulphide reductase activity and the generation of free thiols on the platelet surface. Activation of the PERK and IRE-1 pathway at baseline and after induction of ER stress was increased in PDIA6 deficient platelets (Figure 1B, C). There was striking hypersecretion of PDIA1 (Figure 1D) and α-granule proteins (Figure 1E, F) in response to shear and stimulation with thrombin. PDIA6 CKO mice displayed a prothrombotic phenotype with increased platelet adhesion to fibrinogen under shear (500 s-1) and decreased time to carotid artery occlusion (mean+/SD: 10.8 +/-3.2 min in Pf4Cre+/Pdia6fl/fl compared with 15.3 +/-5.2 min in Pdia6fl/fl, n=8-10, p<0.05). Conclusion: We have identified a role for platelet PDIA6 in attenuating platelet ER stress and secretion. This opens avenues for further study into the role of platelet PDIs in conditions with increased ER stress, such as obesity and diabetes. Figure 1: PDIA6 deficient platelets have increased endoplasmic reticulum (ER) stress and are hypersecretory. A. Western blot of PDIA6 protein in platelets from Pf4Cre+/Pdia6fl/fl mice and control mice (Pdia6fl/fl) showing efficient deletion of PDIA6 in platelets. B. PDIA6 deficient platelets have increased phosphorylation of pEIF2a (PERK phosphorylation pathway) at baseline and after induction of ER stress by thapsigargin, representative image. C. Normalized band intensity (peIF2a/beta actin) in platelets treated with DMSO control or thapsigargin. D. Increased secretion of thiol isomerase PDIA1. E. alpha granule proteins: platelet factor 4 (PF4) and F. von Willebrand factor (vWF) from PDIA6 deficient platelets compared with controls after stimulation with thrombin 0.5 U/ml. n=3-5 Pf4Cre+/Pdia6fl/fl (red boxes) and n=3-5 Pdia6fl/fl mice (grey boxes). Columns are presented as mean+/-SD, *p<0.05, ** p<0.001 by Mann Whitney. Figure 1 Figure 1. Disclosures No relevant conflicts of interest to declare.
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Cheng, He-Peng, Qian Liu, Yang Li, Xiao-Dong Li, and Chao-Yang Zhu. "The Inhibitory Effect of PDIA6 Downregulation on Bladder Cancer Cell Proliferation and Invasion." Oncology Research Featuring Preclinical and Clinical Cancer Therapeutics 25, no. 4 (April 14, 2017): 587–93. http://dx.doi.org/10.3727/096504016x14761811155298.
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Protein disulfide isomerases A6 (PDIA6) belongs to the PDI family. Recently, PDIA6 was found to have a close association with various cancers. However, there has been little investigation into the biological functions of PDIA6 in bladder cancer (BC). In this study, we explored the expression pattern and functional significance of PDIA6 in BC. We found that PDIA6 was overexpressed in BC tissues and cell lines. The in vitro study showed that PDIA6 downregulation significantly inhibited BC proliferation and invasion. In addition, the in vivo experiment demonstrated that PDIA6 downregulation decreased the volume, weight, and metastasis of tumors. Furthermore, PDIA6 downregulation reduced the protein expression of β-catenin, cyclin D1, and c-Myc and thus suppressed the Wnt/β-catenin signaling pathway. In conclusion, we suggest that PDIA6 could be targeted for the treatment of BC.
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Kim, Tae-Wan, Hyang-Hwa Ryu, Song-Yuan Li, Chun-Hao Li, Sa-Hoe Lim, Woo-Youl Jang, and Shin Jung. "PDIA6 regulation of ADAM17 shedding activity and EGFR-mediated migration and invasion of glioblastoma cells." Journal of Neurosurgery 126, no. 6 (August 2016): 1829–38. http://dx.doi.org/10.3171/2016.5.jns152831.
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OBJECTIVEIn patients with glioblastoma, local invasion of tumor cells causes recurrence and shortens survival. The goal of this study was to determine whether protein disulfide isomerase (PDI) A6 regulates migration and invasion of glioblastoma cells and the associated factors.METHODSU87MG cells were treated with either PDIA6 or ADAM17 small interfering RNA (siRNA) fragments or with both types of siRNA fragments, and expression was confirmed by reverse transcription–polymerase chain reaction and Western blot. Migration and invasion were assessed using a wound-healing assay, a Matrigel assay, and an organotypic culture system. After the U87MG cells were treated with siRNAs and epidermal growth factor receptor (EGFR) inhibitors, the expression of matrix metalloproteinase–2 (MMP-2), membrane Type 1-matrix metalloproteinase (MT1-MMP), integrin, phosphorylated focal adhesion kinase (pFAK), and phosphorylated EGFR (pEGFR) was detected by Western blotting and zymography.RESULTSU87MG cell migration and invasion increased significantly after inhibition of PDIA6. The MMP-2 activation ratio and ADAM17 activity (as a sheddase of the proligand) increased, and expression of pEGFR, pFAK, integrin α5β3, and MT1-MMP was induced, compared with control levels. Furthermore, heparin-binding epidermal growth factor (EGFR signaling ligand) was highly expressed in PDIA6-knockdown cells. After siPDIA6-transfected U87MG cells were treated with EGFR signaling inhibitors, expression of pFAK, MMP-2, and MT1-MMP decreased and invasion decreased significantly. Simultaneous double-knockdown of PDIA6 and ADAM17 reduced pEGFR and pFAK expression, compared with control levels.CONCLUSIONSThe authors propose that inhibiting PDIA6 could transduce EGFR signaling by activating and inducing ADAM17 during migration and invasion of U87MG glioblastoma cells. The results of this study suggest that PDIA6 is an important component of EGFR-mediated migration and invasion of U87MG cells. This is the first report of the effects of PDIA6 on migration and invasion in glioblastoma.
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Shen, Chien-Heng, Shui-Yi Tung, Wen-Shih Huang, Kam-Fai Lee, Yung-Yu Hsieh, Meng Chiao Hsieh, Cheng-Nan Chen, and Hsing-Chun Kuo. "Comparative Proteomic Identification of Protein Disulphide Isomerase A6 Associated with Tert-Butylhydroperoxide-Induced Liver Injury in Rat Hepatocytes." Cellular Physiology and Biochemistry 45, no. 5 (2018): 1915–26. http://dx.doi.org/10.1159/000487968.
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Background/Aims: Oxidants are important human toxicants. They have been implicated in the occurrence and development of liver diseases. Increased intracellular tert-butylhydroperoxide (t-BHP) may be critical for oxidant toxicity, and is commonly used for evaluating mechanisms involving oxidative stress, but the method remains controversial. Methods: Primary cultures of hepatocytes as well as human Hep G2 and mouse FL83B liver cells were obtained. Cell viability was measured by annexin V–FITC/propidium iodide and DAPI staining to determine the effects of t-BHP treatment on acute liver injury. A proteomic assay provided information that was used to identify the differentially expressed proteins following t-BHP treatment; immunohistochemistry and western blotting were performed to detect the expression of PDIA6 activity in apoptotic and endoplasmic reticulum (ER) stress pathways. Results: Our results demonstrate that t-BHP treatment of liver cells increased cell cytotoxicity and the generation of reactive oxygen species. This treatment also increased the level of PDIA6; this was validated in vitro and in vivo based on a comparison of t-BHP-treated and -untreated groups. Treatment of mouse liver FL83B cells with t-BHP activated caspase 3, increased the expression of apoptotic molecules, caused cytochrome c release, and induced Bcl-2, Bax and IRE1α/TRAF2 complex formation. t-BHP-dependent induction of apoptosis was accompanied by sustained phosphorylation of the IRE1α/ASK1/JNK1/2/p38 pathways and PDIA6 expression. Furthermore, t-BHP induced liver FL83B cell viability and apoptosis by upregulating the levels of PDIA6; this process could be involved in the activation of the IRE1α/ASK1/JNK1/2/p38 signalling pathways. Conclusions: We conclude that t-BHP induced an apoptosis cascade and ER stress in hepatocytes by upregulation of PDIA6, providing a new mechanism underlying the effects of t-BHP on liver injury.
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Ramos, F. S., L. T. R. Serino, C. M. S. Carvalho, R. S. Lima, C. A. Urban, I. J. Cavalli, and E. M. S. F. Ribeiro. "PDIA3 and PDIA6 gene expression as an aggressiveness marker in primary ductal breast cancer." Genetics and Molecular Research 14, no. 2 (2015): 6960–67. http://dx.doi.org/10.4238/2015.june.26.4.
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Vieujean, S., S. Hu, E. Bequet, C. Salée, C. Massot, N. Bletard, N. Pierre, et al. "P013 Potential Role of Epithelial Protein Disulphide Isomerases in Crohn’s Disease Fibrosis." Journal of Crohn's and Colitis 15, Supplement_1 (May 1, 2021): S134—S135. http://dx.doi.org/10.1093/ecco-jcc/jjab076.142.
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Abstract Background Intestinal fibrosis is a common complication of Crohn’s disease (CD) characterized by an accumulation of fibroblasts differentiating into activated myofibroblasts secreting excessive extracellular matrix. In in-vitro experiments, this myofibroblastic differentiation is elicited by a whole series of factors among which transforming growth factor-β1 (TGF-β1) seems to play a key role. The potential role of the intestinal epithelium in this fibrotic process remains poorly defined. Methods We performed a pilot proteomic study comparing the proteome of surface epithelium isolated by laser-capture microdissection in normal and fibrotic zones of resected ileal CD strictures (13 zones collected in 5 patients). The pro-fibrotic role of selected epithelial proteins was investigated through in-vitro experiments using HT-29 epithelial cells and a CCD-18Co fibroblast to myofibroblast differentiation model. Results Proteomic study revealed an endoplasmic reticulum (ER) stress proteins increase in the epithelium of CD ileal fibrotic strictures, including Anterior gradient protein 2 homolog (AGR2), Protein disulphide isomerase A6 (PDIA6) and Endoplasmic reticulum resident protein 44 (ERP44) which are 3 protein disulphide isomerases. In HT-29 cells, tunicamycin-induced ER stress triggered AGR2, PDIA6, ERP44 as well as TGF-β1 intracellular expression and their secretion. Supernatant of these HT-29 cells, pre-conditioned by tunicamycin (Tm), led to a myofibroblastic differentiation when applied on CCD-18Co fibroblasts. The application of blocking agents for AGR2, PDIA6, ERP44 or TGF-β1 in the supernatant of these Tm-pre-conditioned HT-29 cells, attenuated the myofibroblastic differentiation induced by this supernatant, suggesting a pro-fibrotic role of these secreted epithelial proteins. Conclusion The development of CD fibrotic strictures may involve ER stress in epithelial cells, releasing a whole set of proteins into their environment, including AGR2, PDIA6, ERP44 as well as TGF-β1, which could exercise a pro-fibrotic role through a paracrine action.
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Tufo, G., A. W. E. Jones, Z. Wang, J. Hamelin, N. Tajeddine, D. D. Esposti, C. Martel, et al. "The protein disulfide isomerases PDIA4 and PDIA6 mediate resistance to cisplatin-induced cell death in lung adenocarcinoma." Cell Death & Differentiation 21, no. 5 (January 24, 2014): 685–95. http://dx.doi.org/10.1038/cdd.2013.193.
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Zhou, Ping, Lakshmanan K. Iyer, Hani Hassoun, James E. Hoffman, Heather Landau, and Raymond L. Comenzo. "Cyclin D1 Overexpression In Clonal Plasma Cells In Systemic AL Amyloidosis Is Associated with Differential Expression of Protein Quality Control Genes and Bias In Clonal Germline IgVL donor Gene Use." Blood 116, no. 21 (November 19, 2010): 4043. http://dx.doi.org/10.1182/blood.v116.21.4043.4043.
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Abstract Abstract 4043 Cyclin D1 (CCND1) overexpression in AL plasma cells (PC) is associated with patient characteristics such as production of free immunoglobulin (Ig) light chains (FLC) without an intact M-protein (that is, without partner Ig heavy chains), increased cardiac biomarkers and shorter survival (Amyloid 2010;17(S1):61a; Blood 2008;111:4700; Haematologica 2009;94:380). The molecular ramifications of CCND1 overexpression within AL PC clones have not been described. To study these associations, we used CD138+ AL PC from 69 untreated AL patients at diagnosis for (1) gene expression profiling (GEP, Affymetrix U133A 2.0) (n=16), (2) qRT-PCR to validate GEP findings (n=53), and (3) clonal IgVL germline donor gene identification (n=69) by established methods (Blood 2008;111:549; Blood 2001;98:714). By GEP, all cases displayed significant overexpression of the appropriate isotypic IgVL constant region gene, confirming the preponderance of clonal AL PC. Five cases were CCND1hi and 11 CCND1lo, and a supervised analysis of CCND1hi vs CCND1lo transcriptomes showed that in CCND1hi PC among the most down-regulated genes were IGHG1, IGHG3 and CCND2 while among the most up-regulated ones (after CCND1) were FAM129A, WARS, SEC63, PDIA6 and SEL1L. By RT-PCR all 53 cases used for qRT-PCR displayed prominent amplification of spliced and unspliced XBP1, confirming PC derivation. By qRT-PCR, median CCND1 expression was 1.51 (range, 0–19.36) with 27 cases above (CCND1hi) and 26 below the median (CCND1lo) with clear-cut quartile differences (25% 0.02, 75% 4.78). We examined PDIA6 and SEL1L expression by qRT-PCR, and found that both correlated with CCND1 expression (PDIA6, P=0.018, r=0.452; SEL1L, P=0.038, r=0.395). In addition, PDIA6 and SEL1L values above and below the CCND1 median differed significantly (P=0.01, P=0.04). The genes up-regulated in CCND1hi cases are involved in endoplasmic reticulum (ER) and protein control processes: WARS in protein production, FAM129A in autophagy, SEC63 in ER protein transport, PDIA6 in catalysis of disulfide bonds and SEL1L in modifying misfolded proteins and channeling them to cytosolic proteasomes. We then identified the clonal IgVL germline donor genes in the CCND1hi (n=32) and CCND1lo (n=37) AL PC clones. We knew that CCND1hi clones displayed biased Ig light chain restriction with 10/12 κ and 22/57 λ cases being CCND1hi (p=0.009, Fisher's exact). Surprisingly, we also identified biased λ family use as only 6/27 λ1 and λ2 cases were CCND1hi compared to 16/30 λ3 and λ6 cases (P=0.03). Overall these results confirm that CCND1hi AL PC clones express significantly higher levels of important ER protein quality control genes than CCND1lo clones, possibly due to CCND1hi AL PC clones adapting to the production of FLC without partner Ig heavy chains. Moreover, CCND1hi AL PC clones display a biased clonal IgVL germline donor gene repertoire, raising questions about the origin of CCND1hi clones since germline gene selection is an early and CCND1 overexpression likely a late event in malignant clonal PC emergence. Disclosures: No relevant conflicts of interest to declare.
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Gorasia, Dhana G., Nadine L. Dudek, Helena Safavi-Hemami, Rochelle Ayala Perez, Ralf B. Schittenhelm, Philippa M. Saunders, Sheena Wee, Jon E. Mangum, Michael J. Hubbard, and Anthony W. Purcell. "A prominent role of PDIA6 in processing of misfolded proinsulin." Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics 1864, no. 6 (June 2016): 715–23. http://dx.doi.org/10.1016/j.bbapap.2016.03.002.
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Özenver, Nadire, and Thomas Efferth. "Identification of Prognostic and Predictive Biomarkers and Druggable Targets among 205 Antioxidant Genes in 21 Different Tumor Types via Data-Mining." Pharmaceutics 15, no. 2 (January 28, 2023): 427. http://dx.doi.org/10.3390/pharmaceutics15020427.
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(1) Background: Oxidative stress is crucial in carcinogenesis and the response of tumors to treatment. Antioxidant genes are important determinants of resistance to chemotherapy and radiotherapy. We hypothesized that genes involved in the oxidative stress response may be valuable as prognostic biomarkers for the survival of cancer patients and as druggable targets. (2) Methods: We mined the KM Plotter and TCGA Timer2.0 Cistrome databases and investigated 205 antioxidant genes in 21 different tumor types within the context of this investigation. (3) Results: Of 4347 calculations with Kaplan–Meier statistics, 84 revealed statistically significant correlations between high gene expression and worse overall survival (p < 0.05; false discovery rate ≤ 5%). The tumor types for which antioxidant gene expression was most frequently correlated with worse overall survival were renal clear cell carcinoma, renal papillary cell carcinoma, and hepatocellular carcinoma. Seventeen genes were clearly overexpressed in tumors compared to their corresponding normal tissues (p < 0.001), possibly qualifying them as druggable targets (i.e., ALOX5, ALOX5AP, EPHX4, G6PD, GLRX3, GSS, PDIA4, PDIA6, PRDX1, SELENOH, SELENON, STIP1, TXNDC9, TXNDC12, TXNL1, TXNL4A, and TXNRD1). (4) Conclusions: We concluded that a sub-set of antioxidant genes might serve as prognostic biomarkers for overall survival and as druggable targets. Renal and liver tumors may be the most suitable entities for this approach.
Dissertations / Theses on the topic "PDIA6"
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Tufo, Grégory. "Recherche de nouveaux biomarqueurs de la résistance au cisplatine dans le cancer du poumon : rôle anti-apoptotique des PDIA4 et PDIA6." Versailles-St Quentin en Yvelines, 2012. http://www.theses.fr/2012VERS0017.
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Environ 80% des cancers du poumon sont des carcinomes du poumon non à petites cellules (NSCLC). Les NSCLC posent un enjeu médical majeur en raison de leur forte incidence et du taux de mortalité qu’ils induisent. Ils sont généralement traités avec le cisplatine (CDDP), l'un des agents anticancéreux les plus largement utilisés aujourd'hui. Cependant, après plusieurs mois, des cellules cancéreuses résistantes à l'apoptose induite par le CDDP apparaissent et compromettent le succès de la chimiothérapie. Le CDDP est un composé pro-apoptotique qui se lie l'ADN nucléaire pour former des liaisons inter et intra-chaîne, entraînant une inhibition de la synthèse de l'ADN. Toutefois, des cellules anucléées restent sensibles au CDDP et seulement 1% du CDDP intracellulaire réagit avec l'ADN nucléaire, ce qui suggère que le CDDP peut affecter d'autres cibles. Ainsi, les mécanismes par lesquels le CDDP conduit à la mort cellulaire sont mal définis et de nouvelles cibles intracellulaires sont encore à déterminer. Notre thèse élucide quelques mécanismes de résistance cellulaires et moléculaires à l'apoptose induite par le CDDP dans les cellules cancéreuses du poumon (A549). Un criblage du protéome de réticulum endoplasmique, nous a permis d'identifier un ensemble de protéines, qui sont régulées à la hausse dans les trois lignées cellulaires A549 résistantes au CDDP. Parmi ces protéines, plusieurs isoformes de protéines disulfure isomérases (PDI) ont été confirmées par spectrométrie de masse dans la lignée cellulaire de cancer du poumon A549 et retrouvées par western-blot dans plusieurs lignées cellulaires résistantes au CDDP. L'invalidation pharmacologique et génétique de deux isoformes de PDI restaure la sensibilité au CDDP et rétablit la mort cellulaire dans des lignées cellulaires résistantes. Fait intéressant, nous avons constaté que l'inhibition de PDIA4 induit l'apoptose mitochondriale classique, tandis que celle de PDIA6 déclenche une voie non-canonique de la mort cellulaire. Enfin, nous avons confirmé le rôle de ces PDI dans une lignée cellulaire de cancer ovarien, ce qui indique que les mécanismes de résistance initiés par les PDI peuvent être conservés dans différentes lignées cellulaires cancéreuses. En conclusion, nous avons identifié deux nouveaux acteurs de la résistance au CDDP, dans les cellules cancéreuses du poumon non à petites cellules, qui pourraient devenir de nouveaux biomarqueurs en vue de thérapies et de diagnosticsAbout 80% of lung cancers are non-small-cell lung carcinoma (NSCLC). These NSCLC are a major clinical problem because of their high incidence and mortality rate. They are usually treated with cisplatin (CDDP), one of the most widely used anticancer drugs today. However, following several months, cancer cells, that are resistant to CDDP-induced apoptosis appear, compromising the chemotherapy success. CDDP is a pro-apoptotic compound that binds nuclear DNA to form inter- and intra-chain bonds, causing inhibition of DNA synthesis. However, only 1% of intracellular CDDP reacts with nuclear DNA, suggesting that CDDP can affect other molecules. Thus, mechanisms by which CDDP leads to cell death are poorly defined and novel intracellular targets are still to be determined. Our thesis elucidated some cellular and molecular resistance mechanisms to CDDP-induced apoptosis in lung cancer cells (A549). Using a systematic screen of the endoplasmic reticulum proteome, we have identified a set of proteins, which are up-regulated in three CDDP-resistant A549 cell lines. Among these proteins, several isoforms of proteins disulfide isomerase (PDI) have been confirmed by mass spectrometry in an A549 lung cancer cell line and by western-blot in several CDDP-resistant cell lines. Pharmacological and genetic invalidation of two PDI isoforms rescued the sensitivity to CDDP and restored cell death in resistant cells lines. Interestingly, we found that PDIA4 silencing induce classical mitochondrial apoptosis, whereas PDIA6 triggered a non-canonical cell death pathway. Finally, we confirmed the role of these PDIs in an ovarian cancer cell line, indicating that the PDI-mediated resistance mechanisms may be conserved in various cancer cell lines. In conclusion, we identified two novel actors of CDDP resistance in non-small cell lung cancer cell lines that might be novel biomarkers in therapeutic and diagnosis perspectives
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Eletto, Daniela. "Study of bio-molecar interaction: a) Mapping of the interaction between STAT1 and flavonoids; b) Role of PDIA6-BiP complex in the regulation of the unfolded protein response." Doctoral thesis, Universita degli studi di Salerno, 2012. http://hdl.handle.net/10556/1309.
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2010 - 2011Mapping of the interaction between STAT1 and flavonoids Abstract An experimental approach is described, in the first part of this Ph.D. work, for determining protein-small molecule non-covalent ligand binding sites and protein conformational changes induced by ligand binding. The methodology utilizes a combination of multiple technical approaches: limited proteolysis, MALDI TOF MS, circular dichroism and Surface Plasmon Resonance (SPR) to determine the binding sites in signal transducer and activator of transcription 1, STAT1 (87kDa)-flavonoid (Epigallocatechin-3-gallate, Myricetin and Delphinidin, about 500 Da) non-covalent complex. Comparing relative ion abundances of peptides released from the limited proteolysis of STAT1 and the STAT1-flavonoid complex after 0, 5, 15 and 30 minutes of digestion revealed that the binding of flavonoid induced a significant change in surface topology of STAT1. An increase in ion abundance and a different peptide profile suggest that the flavonoids obstruct the access of the proteases to one or both termini of specific peptides, identifying flavonoids binding region. Taken together, MALDI MS and SPR data led us to assume that the binding sites are close to Tyrosine 701 and that the flavonoids probably act disturbing the phosphorylation of TYR701 and the following dimerization and activation of STAT1. PDIA6-BiP complex: role in the regulation of the unfolded protein response Abstract The unfolded proteins response (UPR) induced in many experimental settings is an extremely strong response that usually leads to cell death rather than to restoration of the ER homeostasis. Because the outcome of UPR signaling determines cell fate, a key unresolved molecular question is how UPR signaling is attenuated. Indeed, it is often under-appreciated that UPR signaling in response to stress is transient and is attenuated. Recently it has been proved that yeast UPR matches its output to the magnitude of the stress by regulating the duration of IRE1 signaling. An ER protein, known as binding immunoglobulin protein (BiP), binding to UPR sensors regulates their deactivation. Our idea, described in the second part of this Ph.D. work, is that there is another luminal ER factor, which interacts with the UPR sensors and is involved in attenuation of their activities. This factor is protein disulphide isomerase 6, PDIA6 (also known as P5), a poorly understood member of the protein disulfide isomerase (PDI) family, whose absence, according to our data, confers hypersensitivity to ER stress because one of its main action is tied to the sensing of UPR, rather than to the consequences of UPR signaling. We thought that PDIA6 uses its protein disulfide isomerase activity to interact specifically with UPR sensors in the ER lumen and attenuate their activities, thus regulating the duration of ER stress signaling. [edited by author]
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Bastos, Sara. "Structural studies of protein Disulfide Isomerases: PDIA1 and PDILT." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=114530.
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AbstractProtein disulfide isomerases (PDIs) family members are essential for proper folding of proteins entering the secretory pathway. Studies of these remarkable enzymes have shown that PDIs catalyze the oxidation, reduction and isomeration of disulfide bonds in the endoplasmic reticulum (ER). PDIs are found and conserved in a wide range of species and are ubiquitously expressed.In all eukaryotes, the ER redox potential must be tightly controlled since a large fraction of proteins within the cell require disulfide-bond formation. How PDIA1 maintains a balance between oxidation and reduction remains an important open question. Previous studies have revealed the tendency of PDIA1 to dimerize, but little is known about the functional relevance of PDIA1 dimerization. Also a recent crystal structure of a dimeric form of human PDIA1 shows that the formation of dimers inhibits substrate binding and therefore may function as a mechanism to regulate PDIA1 activity in the ER. This thesis has found that PDIA1 dimerizes in vivo and proposes that the dimerization of PDIA1 has physiological relevance by auto-regulating its activity. This mechanism would allow the ER to maintain a balance between oxidation and reduction necessary for native disulfide formation. Another enzyme, PDILT, is a testis-specific PDI. PDILT shares a high sequence similarity (32% identity) with PDIA1. Previous studies suggest that they share the same mechanism for binding substrates, which is mediated by a highly conserved hydrophobic pocket. In this thesis, the crystal structure of the PDILT b'-domain is reported and reveals a hydrophobic pocket that contains interesting features for substrate binding. Structural studies of this new PDI-member will help to understand the important role that PDILT plays in the differentiation and maturation on spermatozoids. The study of ER protein folding in the testis may lead to the identification of proteins and pathways associated with male infertility.Résumé Les membres de la famille des protéines disulfures isomérases (PDI) sont essentiels pour le repliement des protéines entrant dans les voies de sécrétion. Les études sur ces enzymes remarquables ont montré que les PDI catalysent l'oxydation, la réduction et l'isomérisation des liens disulfures dans le réticulum endoplasmique (RE). Les PDI sont présentes et conservées chez une large gamme d'espèces et sont exprimées de manière omniprésente.Chez tous les eukaryotes, le potential rédox du RE doit être contrôlé fermement puisqu'une grande fraction des protéines dans la cellule nécessite la formation de liens disulfures. Le mécanisme avec lequel la PDIA1 maintient un équilibre entre l'oxydation et la réduction reste une question importante ouverte. Des études précédentes ont révélé que la PDIA1 a tendance à se dimériser, mais on sait peu de choses sur la pertinence fonctionnelle de la dimérisation de la PDIA1. De plus, une structure cristalline récente d'une forme dimérique de la PDIA1 humaine montre que la formation des dimères inhibe la liaison au substrat et ainsi peut agir comme un mécanisme de régulation de l'activité de la PDIA1 dans le RE. Cette thèse a trouvé que la PDIA1 se dimérise in vivo et propose que la dimérisation de la PDIA1 a une pertinence physiologique en auto-régulant son activité. Ce mécanisme permettrait au RE de maintenir un équilibre entre l'oxydation et la réduction nécessaires pour la formation de liaisons disulfures natives.Une autre enzyme, la PDILT, est spécifique pour le testicule. La PDILT partage une similitude de séquence importante (32%) avec la PDIA1. Des études précédentes suggèrent qu'elles partagent le même mécanisme de liaison des substrats qui est médiée par une poche hydrophobe hautement conservée. Dans cette thèse, la structure cristalline du domaine b' de la PDILT est reportée et révèle une poche hydrophobe qui contient des caractéristiques intéressantes pour la liaison du substrat. Des études structurelles de ce nouveau membre de PDI aideront à comprendre le rôle important que joue la PDILT dans la différentiation et la maturation des spermatozoïdes. L'étude du repliement des protéines du RE dans le testicule pourrait mener à l'identification de protéines et voies associées à l'infertilité masculine.
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Chen, Jiaxuan. "The role of Pdia3 in vitamin D signaling in osteoblasts." Diss., Georgia Institute of Technology, 2012. http://hdl.handle.net/1853/50147.
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1a,25-Dihydroxyvitamin D3 (1a,25(OH)2D3) is a major functional metabolic form of vitamin D. 1a,25(OH)2D3 has drawn increasing attention due to its functions in addition to maintaining calcium phosphate homeostasis. It directly regulates mineralization by osteoblasts, matrix production and remodeling by chondrocytes, and contraction of cardiomyocytes. 1a,25(OH)2D3 and its analogues have shown beneficial effects in treating multiple sclerosis, diabetes and various types of cancer. In order to maximize the pharmaceutical potential of 1a,25(OH)2D3, a better understanding its cell signaling pathway is necessary. 1a,25(OH)2D3 regulates osteoblasts through both classical nuclear vitamin D receptor (nVDR) mediated genomic effects and plasma membrane receptor-mediated rapid responses. The identity of the plasma membrane receptor for 1a,25(OH)2D3 is controversial. Protein disulfide isomerase associated 3 (Pdia3) has been hypothesized as one of the putative plasma membrane receptors for 1a,25(OH)2D3. The overall goal of this thesis was to understand the general role and the molecular mechanism of Pdia3 in 1a,25(OH)2D3-initiated rapid responses, and to determine the role of Pdia3 and its dependent signaling in osteoblast biology. The results show that Pdia3 is required for membrane-mediated responses of 1a,25(OH)2D3. Moreover, both Pdia3 and nVDR are critical components of the plasma membrane receptor complex for 1a,25(OH)2D3. Finally, Pdia3 and signaling via Pdia3 regulate osteoblast differentiation and mineralization. Taken together, this study demonstrates the role of Pdia3 in rapid responses to 1a,25(OH)2D3 and osteoblast biology, reveals the unexpected complexity of the 1a,25(OH)2D3 plasma receptor complex and opens the new target, Pdia3, for pharmaceutical application and tissue engineering.
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Silva, Thaís Larissa Araujo de Oliveira. "Estudo da rota de externalização da dissulfeto isomerase protéica (PDIA1) em células endoteliais." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/5/5131/tde-09112015-113347/.
Full textAbstract:
Dissulfeto isomerase protéica (PDIA1 ou PDI) é uma chaperona e ditiol-dissulfeto oxido-redutase residente do reticulo endoplasmático (RE). PDI é essencial à regulação da proteostase por ter função no enovelamento oxidativo de proteínas e na via de degradação associada ao RE (ERAD). Além disso, PDI interage fisicamente e regula a atividade de NADPH oxidases, e fora da célula é um regulador redox essencial à atividade de proteínas extracelulares. Este pool epi/pericelular da PDI (pecPDI) regula função de proteínas de membrana/secretadas, como integrinas, glicoproteínas gp120 do virus HIV e outras, com múltiplas funções que incluem: trombose, ativação plaquetária, adesão celular, infecção viral e remodelamento vascular. A rota de externalização da PDI permanece obscura, e seu conhecimento pode indicar mecanismos dos efeitos (fisio)patológicos da PDI. A secreção da PDI pela rota RE-Golgi foi sugerida em células endoteliais infectadas pelo vírus da dengue, células pancreáticas e tireoideanas. No entanto, uma varredura sistemática das possíveis rotas de externalização da PDI não foi previamente realizada. Neste estudo, mostramos que células endoteliais (EC) externalizam constitutivamente, por rotas distintas, dois pools de PDI, de superfície celular e solúvel, enquanto na EC não estimulada PDI não foi detectada significativamente em micropartículas. PDI externalizada corresponde a ca.1,4% do pool total de PDI celular. Tanto a PDI de superfície celular como a solúvel foram majoritariamente secretadas pela via de secreção não-convencional do tipo IV independente de GRASP. Contudo, a via de secreção clássica também contribui para externalização basal da PDI de superfície celular, mas não da solúvel basal ou estimulada por PMA, ATP e trombina indicando que todas envolvem escape do Golgi. Além disso, a externalização constitutiva da PDI de superfície em célula muscular lisa vascular também ocorre por via independente de Golgi. Externalização da PDI não foi detectavelmente mediada pela secreção não-convencional do tipo I, II, III, lisossomos secretórios, endossoma de reciclagem e transporte ativo (dependente de ATP) em EC. Considerando que chaperonas são vias essenciais de resposta a estresses, investigamos o efeito de estresse do RE e choque térmico na pecPDI. Estresse do RE não altera a PDI de superfície celular, mas aumenta PDI solúvel. Ambos os pools de PDI não foram alterados por choque térmico, embora a recuperação desse estresse diminua a secreção de PDI. Estes dados sugerem que a liberação de PDI é um processo regulado, dependente da natureza do estresse. Bloqueio da síntese de proteínas com cicloheximida não altera pecPDI, indicando que PDI recém-sintetizada não é preferencialmente externalizada e que o tráfego da PDI independe de outras proteínas recém-sintetizadas. Um aspecto importante do estudo foi indicar uma resiliência da pecPDI à modulação individual de distintas vias secretoras, consistente com uma estrita auto-regulação e possibilidade de vias sinérgicas e complementares. Estes resultados indicam que a externalização da PDI de superfície e PDI secretada possam ser externalizadas por mecanismos independentes. Estes processos compõem um processo regulado estritamente, consistente com papel homeostático da pecPDIProtein disulfide isomerase (PDIA1 or PDI) is dithiol-disulfide oxireductase chaperone resident in the endoplasmic reticulum (ER). PDI is essential for proteostasis, due to its support of oxidative protein folding and ER-associated protein degradation (ERAD). In addition, PDI associates with NADPH oxidase(s) and regulate its activity, while outside of the cell, PDI redox-dependently modulates extracellular proteins. This epi/pericellular PDI (pecPDI) pool is known to regulate membrane/secreted proteins such as integrins, HIV glycoprotein gp120 and others, with functions that involve thrombosis, platelet function, cell adhesion, viral infection and vascular remodeling. PDI externalization route remains enigmatic and its elucidation can help understand some (patho)physiological PDI effects. An ER-Golgi route for PDI secretion has been as described on dengue virus-infected endothelial cells pancreatic and thyroid) cells. However, none of these papers addressed PDI secretion routes in a systematic fashion. Here, we show that endothelial cells (EC) constitutively externalize, through different routes, two PDI pools, a cell-surface and a secreted one, while in nonstimulated ECs PDI was not significantly detected in microparticles. Externalized PDI corresponds to < 2% of total cellular PDI pool. Both cell-surface and soluble PDI were predominantly externalized through unconventional type IV GRASP-independent pathway(s). However, the classical secretory pathway also contributes to basal cell-surface, but not soluble, PDI externalization, as PMA, ATP or thrombin-stimulated secretion also involve Golgi bypass. Furthermore, constitutive cell-surface PDI externalization in vascular smooth muscle cells also occurs in a Golgi-independent way. PDI externalization was not detectably mediated by non-conventional type I, II and III secretion routes, secretory lysosomes, recycling endosomes and ATP dependent active transport in EC. Since chaperones are essential for cellular stress response, we assessed the effects of ER stress and heat-shock on pecPDI. ER stress did not affect cell-surface PDI but increased the soluble pool. Both PDI pools were unaltered by heat shock, while stress recovery decreased PDI secretion. These data suggest that PDI release is finely tuned and dependent on the type of stress. Blockade of protein synthesis with cycloheximide did not change pecPDI levels, suggesting that newly-synthesized PDI is not preferentially externalized and that PDI traffic does not require newly-synthesized proteins. An important aspect of the study was the evidence for pecPDI resilience to individual modulation of distinct secretion routes, consistent with strict auto-regulation and possible synergic or complementary pathways. Overall, our data suggest that cell-surface and secreted PDI pool externalization are regulated through independent mechanisms, which in both cases involve Type IV non-conventional routes, with some minor contribution of Golgi-dependent secretory pathway. These patterns compose a strictly regulated process, consistent with an important homeostatic role for pecPDI
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Pyburn, Jaeden, and Matthew Keasey. "Development of PDIA3 and VDR Knockout Human Osteosarcoma SaOs-2 Cells Using CRISPR-Cas9." Digital Commons @ East Tennessee State University, 2020. https://dc.etsu.edu/asrf/2020/presentations/59.
Full textAbstract:
Intro: Hypovitaminosis D (vitamin D deficiency) has been observed in ageing patients with brain calcification and loss of the vitamin D receptor leads to abnormal calcification of the basal ganglia and thalamus. We have found that vitamin D can reverse calcification of human osteosarcoma SaOs-2 cells in vitro, in apparent contrast to its known effects of increasing bone strength in patients with Rickets and Osteomalacia. Vitamin-D functions through binding to two Vitamin-D responsive proteins; the vitamin D receptor (VDR) and Protein Disulfide isomerase A3 (PDIA3). The aim of this project was to establish VDR and PDIA3 knockout SaOs-2 cells using CRISPR-Cas9 technology. Methods: We designed guide RNA (gRNA) sequences against PDIA3 and VDR using ChopChop, selecting only gRNAs with low predicting non-specific binding probabilities. These gRNA sequences were ordered as oligonucleotides and dimerised before directional cloning into a Cas-9 plasmid. Plasmids were amplified in DH5 E. coli and purified before transfection into SaOs-2 cells together with a plasmid containing the puromycin resistance gene. Cells were treated with puromycin (1 ug/ml) for 4 days to eliminate non-transfected cells. SaOs-2 cells were maintained for 7 days before being passaged and plated for colony selection. Results: Real Time quantitative PCR showed 1 SaOs-2 clone had non-detectable levels of PDIA3 while 4 out of 6 clones had no detectable VDR mRNA relative to wild type cells. Two clones were selected for further analysis. Western blotting of these two clones probing for VDR and PDIA3 confirmed there were no detectable levels of these two proteins. Conclusion: We successfully knocked out expression of the Vitamin-D receptors VDR and PDIA3 in SaOS2 cells. These cells will be used for further study of Vitamin-D related signaling.
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Linden, Liana de Salles van der. "Avaliação da proteína disulfeto isomerase A1 (PDIA1) como marcador para a qualidade seminal em garanhões." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2017. http://hdl.handle.net/10183/182414.
Full textAbstract:
O período de puberdade, em equinos, define-se pela aparição de espermatozóides maduros no ejaculado de animais jovens, bem como a maturação das funções endócrinas. Os espermatozóides precisam passar por um processo de maturação no epidídimo. Para se obter marcadores moleculares que permitam estabelecer a fertilidade de um garanhão, é necessário um melhor conhecimento das proteínas presentes em espermatozóides imaturos e maduros, uma das quais é a PDI (proteína dissulfeto-isomerase). A PDI foi descrita também como importante marcador de fertilidade no plasma seminal e espermatozóides de diversas espécies. O objetivo deste trabalho foi identificar a presença da PDI no epidídimo equino durante a puberdade, e quantificá-la no fluido e espermatozóides epididimários. Visou verificar a presença da PDI no plasma seminal e espermatozóides ejaculados, em garanhões férteis e subférteis. Foram realizados dois experimentos: Experimento 1: utilizou-se 22 equinos Crioulos saudáveis, castrados e divididos em três grupos: G1: potros até 24 meses, G2: de 25-36 meses e G3: a partir de 36 meses. Imediatamente após a castração, foi efetuada a dissecação do epidídimo para a coleta de fluido epididimário, o qual foi separado dos espermatozoides por centrifugação a 800 g por 10minutos. O sobrenadante foi removido, e criopreservado a -196º C. Os espermatozóides foram ressuspendidos em PBS e armazenados a -196º C. Para a dosagem de proteína das amostras, utilizou-se o método de Kit BCA espectrofotômetro e a eletroforese em gel de poliacrilamida a 10% SDS- Page.Para imunodetecção das proteínas, efetuou-se a incubação do anticorpo primário específico por no mínimo, 6 horas a 4º C, e incubação com anticorpo secundário conjugado com peroxidase anti-igG de camundongo ou anti-IgG de rato. Para a visualização das bandas foi utilizado o Kit de ECL em filmes de raio-X, e as bandas quantificadas pela utilização do software livre ImageJ. A PDI foi identificada nos três grupos avaliados na análise proteômica do fluido , e espermatozoides epididimários, sendo sua quantidade inferior no G1 em relação aos grupos dois (2) e três (3). Em conclusão, a expressão da PDI nos espermatozóides e fluido epididimários de potros castrados cirurgicamente, aumenta conforme o animal atinge a maturidade sexual. Experimento 2- Utilizou-se 12 garanhões adultos que já haviam sido submetidos à pelo menos duas temporadas de monta na região da Campanha do Rio Grande do Sul, efetuando-se quatro (4) coletas de cada garanhão, fora da estação de monta, respeitando-se um intervalo de 48h entre coletas. Imediatamente após a coleta, analisou-se motilidade, vigor e concentração com auxílio de microscópio óptico e retirou-se uma alíquota de sêmen para avaliação das patologias espermáticas. A partir dos dados obtidos, os garanhões foram divididos em dois grupos: Grupo 1: motilidade não inferior a 70%, tendo-se uma média de 76,04±5,89% e histórico reprodutivo de temporadas anteriores com índice de prenhez mínimo por temporada de 80%. Grupo 2: motilidade igual ou inferior a 30%, com média de 11,83±11,21%, e histórico reprodutivo de temporadas anteriores de índices de prenhez inferiores a 35%. Efetuadas as análises, as mostras foram centrifugadas a 800 g por 10 minutos para separar o plasma seminal, de mesma forma que no Exp. 1. Os pellets passaram por ressuspensão em PBS gelado e posteriormente armazenados a -196º C. As amostras foram submetidas à dosagem das proteínas, para serem submetidas à eletroforese, utilizando-se géis de poliacrilamida a 10% SDS-Page.Para imunodetecção das proteínas, efetuou-se a incubação do anticorpo primário específico por no mínimo, 6 horas a 4º C, e incubação com anticorpo secundário conjugado com peroxidase anti-igG de camundongo ou anti-IgG de rato. Na visualização das bandas foi utilizado o Kit de ECL em filmes de raio-X, e as bandas foram quantificadas pela utilização do software livre Image J. Ao analisar a presença da PDI no plasma seminal dos garanhões, verificou-se sua expressão em ambos os grupos; porém não houve diferença de expressão da PDI entre eles(p˂0,05). Não houve também relação da PDI com motilidade, nem com a concentração espermática. Considerando os dados obtidos no presente experimento, não foi possível relacionar a PDI com qualidade espermática e assim considerá-la como um potencial marcador de fertilidade em equinos. São necessários mais estudos que possam envolver outros fatores moleculares inclusive as demais proteínas da família das PDIs.Puberty, in the equine species, may be defined by the appearance of mature spermatozoa in young animals´ ejaculates, as well as endocrine function maturation. One of the proteins found in immature and mature spermatozoa is PDI (protein dissulfide-isomerase). PDI was also described as an important fertility marker both in seminal plasma and sperm of many species. is responsible for rearranging dissulfide bonds, necessary for sperm adhesion proteins to link to the oocyte. The aim of this work was to identify PDI in equine epididymis during puberty, and quantify it in epididymal sperm and fluid of fertile and subfertile sperm. Two experiments were performed. Experiment 1-twenty-two healthy Crioulo colts were surgically castrated, and divided in three groups: G1: until 24 months; G2: from 25-36 months and G3: more than 36 months. Immediately after castration, testicles were measured, weighed, and the epididymis was dissecated for epididymal fluid collection, which was centrifuged at 800 g for 10minutes to separate epididymal fluid from sperm. Supernatant was removed, and cryopreserved at -196º C. Sperm were re-suspended in PBS and stored at -196º C. Protein dosing of samples was performed with BCA Kit and electrophoresis at 10% SDS-Page. To detect proteins, primary antibody was incubated for at least 6 hours at 4º C, and then incubation with secondary antibody conjugated with anti-mouse IgG or anti-rat IgG. To see bands, ECL Kit in X-ray films was used, and the bands quantified with softwareImageJ. In the three groups PDI was identified, in epididymal fluid and epididymal sperm, but in smaller amount in G1 when compared with Groups 2 and 3. In conclusion, expression of PDI in epididymal fluid and sperm of surgically castrated colts, increases as the animal attains sexual maturity. Experiment 2- The aim of this work was to verify the presence of PDI in equine seminal plasma and sperm, quantify it and to compare its expression on seminal plasma from fertile and subfertile stallions. Twelve adult stallions with at least two breeding season were used. For the study, four collections of each animal were performed. Immediately after collection, analysis of motility, velocity, concentration and sperm morphology were performed. Stallions were divided in two groups, according to the semen analysis and previous breeding history: Group 1: motility greater than 70% and previous history of pregnancy rates higher than 80%; Group 2: sperm motility less or equal than 30% and breeding history of less than 35% of pregnacy per season. After the analysis, samples were centrifuged at 800 g/10minutes to remove seminal plasma. Samples were prepared as described in Exp. 1. The expression of PDI in seminal plasma was seen in both groups, but with no statistical difference between them. There was no correlation of PDI with sperm motility or concentration. According to these findings, it is not possible to consider PDI as a fertility marker in stallions. More research is needed, involving other mollecular factors, including other PDIs family proteins.
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Bougnoux, Anne-Claire. "Identification et caractérisation de biomarqueurs associés à la progression tumorale du mélanome malin cutané : implication de TRAP1 et PDIA4." Thesis, Montpellier 1, 2014. http://www.theses.fr/2014MON1T014.
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Bien que le mélanome malin cutané (MMC) ne représente que 10% des cancers cutanés, il en est la forme la plus agressive et est responsable de 90% des décès dus aux cancers de la peau. Son incidence ne cesse d'augmenter ces dernières années et atteint 10.8 cas pour 100 000 habitants chez les hommes et 11 chez les femmes. La survie médiane des patients atteint de mélanome à un stade avancé est de 6.2 mois et seulement 1 patient sur 4 est encore en vie à 1an. Le diagnostic du mélanome est uniquement histopathologique et seul l'examen de la biopsie permet de le poser définitivement. De la même façon, il n'existe pas de marqueurs pronostiques associés au mélanome malin cutané. Seuls des facteurs histologiques tels que l'épaisseur de la tumeur, le degré d'invasion ou encore l'envahissement ganglionnaire ont été validés par l'American Joint Committee on Cancer et servent de facteurs pronostiques. Cependant, bien qu'ils permettent d'évaluer le risque métastatique, leur valeur pronostique est encore très insuffisante. L'objectif de ma thèse est d'identifier et de caractériser les protéines associées à la progression tumorale du MMC. Grâce à une approche protéomique quantitative de type iTRAQ par nanoLC MS/MS, j'ai pu identifier et caractériser 2242 protéines dans un modèle cellulaire de progression tumorale du mélanome. La surexpression de huit de ces protéines dans les lignées tumorales par rapport à la lignée normale a été validée par western blot. Deux d'entre elles, TRAP1 et PDIA4 ont ensuite été validées en immunohistochimie comme marqueurs associés à la progression tumorale dans le MMC. Dans un second temps, l'implication de ces deux protéines dans les mécanismes de carcinogénèse du mélanome a été étudiée. L'inhibition de TRAP1 et de PDIA4 induit une diminution de la migration et de la viabilité de la lignée cellulaire métastatique de mélanome 1676. Enfin, la sous-expression dePDIA4 induit une inhibition du complexe Cycline D/CDK4 provoquant un blocage des cellules en phase G0/G1. Ce blocage passerait par la voie PERK liées au stress du réticulum endoplasmiqueAlthough cutaneous malignant melanoma (CMM) represents only 10% of skin cancers, it is the most aggressive form with 90% of deaths from skin cancer. The Incidence rate is increasing in recent years to 10.8 cases and 11 cases per 100000, in men and women respectively. The prognosis of metastatic melanoma is poor, with a median survival of only 6.2 months. Histopathologic examination remains the gold standard for melanoma diagnosis. There are no prognostic markers associated with CMM. Only histological factors such as tumor thickness, level of invasion, or lymph node involvement have been validated by the American Joint Committee on Cancer and are currently used as prognostic factors. However, although these histological factors are used to assess the risk of metastasis development, their prognostic value is still very low. The aim of my PhD work is to identify and characterize biomarkers associated with tumor progression of CMM. Using a quantitative proteomics approach (iTRAQ and nanoLCMS/MS), I was able to identify and characterize 2242 proteins in a cellular model of melanoma tumor progression. The overexpression of eight proteins in tumor cell lines compared to the normal cell line was validated by immunoblotting. Among these proteins, TRAP1 and PDIA4 were then validated by immunohistochemistry as markers associated with tumor progression in the CMM. Secondly, the involvement of these two proteins in the mechanisms of melanoma carcinogenesis has been studied. The inhibition of TRAP1 and PDIA4 induces a decrease in migration and viability of the metastatic melanoma cell line. Finally, PDIA4 under expression induce an inhibition of cyclin D/Cdk4 complex leading to G0/G1 cell-cycle arrest dependant of endoplasmic reticulum stress by the PERK pathway
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Goncalves, Renata. "Interactions entre la signalisation estrogénique et la vitamine D dans les cellules testiculaires." Thesis, Normandie, 2018. http://www.theses.fr/2018NORMC411/document.
Full textAbstract:
La 1α,25(OH)2 vitamine D3 (1,25-D3) est synthétisée à partir du cholestérol par l'exposition solaire de la peau. Les effets de cette hormone sont médiées par le récepteur de la vitamine D (VDR) dans le noyau et à la membrane plasmique, et avec le récepteur PDIA3 ils médient des effets génomiques et non-génomique. La vitamine D joue un rôle important dans la reproduction, puisque la réduction de la fertilité a été observée chez les rats déficients en vitamine D. L'estradiol (E2) est synthétisé à partir de la testostérone par l'enzyme aromatase (CYP19). L’E2 a des effets génomiques et non génomiques médiée par les récepteurs ESR1, ESR2 et GPER. L’objectif de ce travail a été d’étudier l’effet de l’E2 sur le métabolisme et les voies de signalisation de la vitamine D dans des testicules des rats à différents âges ainsi qu’une perturbation éventuelle initiée par un perturbateur endocrinien à activité estrogénique, le Bisphénol A (BPA). Dans le première axe de travail, trois protocoles expérimental (PE) ont été réalisés, où l’E2 et le BPA ont été administrés: traitement de J15pp à J30pp et euthanasie immédiate à J30 (PE1), traitement de J15 à J30 et euthanasie différée à J75 (PE2) et traitement à l’âge adulte de J60 à J75 et euthanasie immédiate à J75 (PE3). Dans le PE1, le traitement avec l’E2 a diminué l'expression du CYP27A1. L’E2 et le BPA ont diminué l'expression du VDR. Cet effet n'a pas été vérifié dans l'expression de la protéine VDR. Dans le PE2, l’E2 a augmenté l'expression des gènes VDR, PDIA3 et CYP27A1, et l'expression de la protéine VDR et CYP27A1. Les traitements n’ont eu aucun effet dans le PE3, ce qui indique qu’un traitement en période prépubère entraîne à la fois un effet immédiat et différé alors que le traitement à l’âge adulte semble sans effet. Dans le deuxième axe de travail, des effets non-génomiques du BPA ont été étudiés par la technique d’afflux de 45Ca2+ dans les testicules de rat prépubères. Le BPA a stimulé l’afflux de 45Ca2+ de manière un peu pareille avec les effets de l’E2. Cet effet semble ne pas impliquer les récepteurs classiques des estrogènes, mais semble se produire de manière compatible avec l'activation d'un récepteur couplé à la protéine G, comme le GPER. Cet effet se produit par la modulation de la fonction des canaux ioniques, comme des canaux potassiques, TRPV1 et des canaux chlorés. Aussi le BPA module le calcium du stock intracellulaire par l’inhibition de la SERCA et l’activation du récepteur IP3. Également des protéines kinases PKA, PKC, MEK et p38MAPK participent de l’effet du BPA, qui pourrait déclencher un cross talk avec les voies de signalisation nucléaires résultant la médiation des réponses génomiques. Dans le troisième axe de travail, l'expression de certains gènes impliqués dans le métabolisme et la signalisation de 1,25-D3 et E2 a été analysée dans des cellules de Leydig. La 1,25-D3 a diminué l'expression du CYP27A1, un effet qui a également été observé lorsque les cellules étaient co-incubées avec l'E2. L’E2 a diminué l'expression des gènes ESR1 et CYP19. Les deux hormones ont démontré un mécanisme de retours négatifs sur leur métabolisme dans ces cellules. Des effets non génomiques ont été étudiés dans ces cellules, où l’E2 semble avoir un effet inhibiteur tandis que la 1,25-D3 a stimulé l'afflux de 45CA2+. A partir de ces résultats, nous pouvons affirmer que la 1,25-D3, l’E2 et le BPA ont des effets moléculaires importants dans le système reproducteur masculin, par l'expression génique des récepteurs et des enzymes impliqués dans le métabolisme des hormones 1,25-D3 et E2. De plus, les résultats obtenus renforcent la théorie selon laquelle il existe une relation entre les voies de signalisation de la 1,25-D3 et l’E2. Comme la 1,25-D3 et l'E2, le BPA stimule également les effets non-génomiques impliqués dans la signalisation du calcium1α,25-dihydroxyvitamin D3 (1,25-D3), the active form of vitamin D, is synthetized from cholesterol by skin exposure to the sun. This hormone’s actions are mediated by vitamin D receptor (VDR) in the nucleus and in the plasma membrane, resulting in genome actions like gene expression regulation. VDR can also be found in the plasmatic membrane, and together with PDIA3 receptor they mediate 1,25-D3 non-genomic actions. Vitamin D has an important role in reproductive function, since fertility reduction was observed in vitamin D deficient rats, as well as VDR and 1α-hydroxylase deficiency. In these animals, calcium and estrogen supplementation was able to reverse the deleterious effects in reproductive function, indicating that there is a relation between 1,25-D3 and estrogens signalling pathways. Estradiol (E2) is synthetized from testosterone by aromatase enzyme (CYP19). E2 is found in high levels in the male reproductive function, and like 1,25-D3 can induce genomic and non- genomic actions, mediated by ESR1, ESR2 and GPER receptors. Bisphenol A (BPA) is a xenoestrogen utilized in plastic industry, capable of modulating the endocrine system through E2 receptors. The aim of this work was to study metabolism and signaling pathways interactions between 1,25-D3 and E2, as well as BPA influence in testicular cells. In the first line of work, three treatment protocols (TP) were realized, where E2 and BPA were administrated in rats between 15th and 30th days, were a portion of the animals were euthanized at the last day of treatment (TP1) and another portion was kept alive after the treatment until euthanized at adult age with 75 days (TP2). A third animal group also received the same treatments when adults (TP3). In TP1, E2 treatment decreased CYP27A1 gene expression. E2 and BPA decreased VDR gene expression. This effect was not verified in VDR protein expression. In TP2, E2 increased VDR, PDIA3 and CYP27A1 gene expression, and VDR and CYP27A1 protein expression, indicating a compensatory effect over gene expression inhibition in prepubertal age. In TP3, treatments did not change gene expression, indicating that prepubertal age is more susceptible to estrogen exposure. In the second line of work, non-genomic effects of BPA were studied through 45Ca2+ influx in prepubertal rat testis. BPA stimulated 45Ca2+ influx in a similar manner to E2. This effect was independent of classical ERs, consistent with a G protein-coupled receptor mechanism, probably GPER. This effect involves the modulation of ionic channels, such as K+, TRPV1 and Cl- channels. Furthermore, BPA is able to modulate calcium from intracellular storages by inhibiting SERCA and activating IP3 receptor/Ca2+ channels at the endoplasmic reticulum and activate kinase proteins, such as PKA and PKC. The rapid responses of BPA on calcium influx could, in turn, trigger a cross talk by MEK and p38MAPK activation and also mediate genomic responses. In the third line of work, the expression of some genes involved in 1,25-D3 and E2 metabolism and signalling were analysed in Leydig cells. 1,25-D3 decreased CYP27A1 gene expression, an effect that was also observed when cells were coincubated with 1,25-D3 and E2. E2 decreased ESR1 and CYP19 gene expression. Both hormones demonstrated an negative feedback mechanism over their on metabolism in these cells. Non-genomic effects were also studied in these cells, where E2 seems to have an inhibitory effect while 1,25-D3 was able to stimulate calcium influx. From these results we can conclude that 1,25-D3, E2 and BPA have important molecular effects in the male reproductive system, through gene expression control over receptors and enzymes involved in the metabolism of the steroid hormones studied. These results also reinforce the theory that there is a relationship between 1,25-D3 and E2 signalling pathways, as well as 1,25-D3, E2 and BPA also have non-genomic actions in calcium signalling
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Doroudi, Maryam. "Essential roles of Pdia3/PLAA receptor complex and CaMKII IN 1α,25(OH)₂D₃ and Wnt5a calcium-dependent signaling pathways in osteoblasts and chondrocytes." Diss., Georgia Institute of Technology, 2014. http://hdl.handle.net/1853/53427.
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The vitamin D metabolite 1,25-dihydroxyvitamin D3 [1α,25(OH)2D3] plays an important role in the regulation of musculoskeletal growth and differentiation. 1α,25(OH)2D3 mediates its effects on cells, including chondrocytes and osteoblasts, through the classical nuclear 1α,25(OH)2D3 receptor. Additionally, recent evidence indicates that several cellular responses to 1α,25(OH)2D3 are mediated via a rapid, calcium-dependent membrane-mediated pathway. These actions of 1α,25(OH)2D3 can be blocked by antibodies to protein-disulfide isomerase family A, member 3 (Pdia3), indicating that it is part of the receptor complex; however, the pathway which is activated by this receptor is not fully understood. The overall goal of this thesis was to examine the roles of phospholipase A2 activating protein (PLAA) and calcium calmodulin-dependent kinase II (CaMKII) in 1α,25(OH)2D3 rapid membrane-mediated signaling. We further investigated the interaction between two pathways regulating growth plate cartilage and endochondral bone formation, 1α,25(OH)2D3 and Wnt5a, at the receptor complex level. Results indicated that PLAA was required for mediating 1α,25(OH)2D3 signal from Pdia3. Furthermore, CaM and CaMKII were identified as mediators of 1α,25(OH)2D3-stimulated PLAA-dependent activation of cPLA2 and PKCα, and downstream biological effects. Wnt5a and 1α,25(OH)2D3 are important regulators of endochondral bone formation. This study demonstrated that 1α,25(OH)2D3 and Wnt5a mediate their effects via similar receptor components in osteoblasts and chondrocytes suggesting that these pathways may interact.
Books on the topic "PDIA6"
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Katalog pameran buku-buku Aceh, tanggal 27 Agustus s/d 3 September 1988 di PDIA-Banda Aceh. Banda Aceh: Panitia Pusat Pekan Kebudayaan Aceh-3, Sub Seksi Pameran Buku-Buku Aceh, 1988.
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Andrews, Matt. How Do Governments Build Capabilities to Do Great Things? Edited by Carol Lancaster and Nicolas van de Walle. Oxford University Press, 2016. http://dx.doi.org/10.1093/oxfordhb/9780199845156.013.34.
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Governments can play great roles, resolving festering problems and opening new pathways for progress. Examples are numerous and raise an important question: How do governments build the capabilities required to do great things? This chapter identifies ten cases of such governments to answer four dimensions of this question: how do governments to ramp up their capability? Who leads these interventions ?, When do they occur, and why? How changes implemented to ensure they yield sustainable results? The chapter suggests two sets of answers to these concerns, combining rival theories that explain how governments enhance capabilities and strengthen their role: “solution- and leader-driven change” (SLDC) and “problem-driven iterative adaptation” (PDIA). It proposes using these two theories in future research about how governments foster the kinds of achievements one could call great and argues this research should employ a version of theory-guided process tracking (TGPT) called systematic process analysis.
Book chapters on the topic "PDIA6"
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Lam, Shing Tat Theodore, and Chinten James Lim. "Cancer Biology of the Endoplasmic Reticulum Lectin Chaperones Calreticulin, Calnexin and PDIA3/ERp57." In Cellular Biology of the Endoplasmic Reticulum, 181–96. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-67696-4_9.
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Petrevska Nechkoska, Renata, Arber Hajrizaj, Olga Arsic, Klejda Harasani, Milan Stojanovic, Sabahudin Mujkić, Samir Beharic, et al. "PDIA in the Balkans: The Western Balkans Alumni Association (WBAA) as Positive Deviance." In Contributions to Management Science, 237–67. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-11065-8_9.
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Ogden, Timothy. "RCTs in Development Economics, Their Critics and Their Evolution." In Randomized Control Trials in the Field of Development, 126–51. Oxford University Press, 2020. http://dx.doi.org/10.1093/oso/9780198865360.003.0006.
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The use of Randomized Control Trials (RCTs) in development economics has attracted a consistent drumbeat of criticism, but relatively little response from so-called randomistas (other than a steadily increasing number of practitioners and papers). This chapter systematizes the critiques and discusses the difficulty in responding directly to them. Following this the chapter applies prominent RCT critic Lant Pritchett’s PDIA framework to illustrate how the RCT movement has been responsive to the critiques if not to the critics through a steady evolution of practice. Finally, the chapter assesses the current state of the RCT movement in terms of impact and productivity.
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Clark, David. "A Culture Transformed? Post-PDIA Progress in Palliative and End-of-Life Care." In Transforming the Culture of Dying, 223–52. Oxford University Press, 2013. http://dx.doi.org/10.1093/acprof:oso/9780199311613.003.0009.
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Nemere, I., N. Garbi, and GJ Hammerling. "Intestinal Cell Phosphate Uptake and the Targeted Knockout of the 1,25D3-MARRS Receptor/PDIA3/Erp57." In The Endocrine Society's 92nd Annual Meeting, June 19–22, 2010 - San Diego, P1–154—P1–154. Endocrine Society, 2010. http://dx.doi.org/10.1210/endo-meetings.2010.part1.p4.p1-154.
Full textConference papers on the topic "PDIA6"
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Chamberlain, N., B. Korwin-Mihavics, E. Nakada, S. Bruno, D. Heppner, D. G. Chapman, S. M. Hoffman, et al. "Lung Epithelial PDIA3 Plays a Critical Role in Influenza infection." In American Thoracic Society 2019 International Conference, May 17-22, 2019 - Dallas, TX. American Thoracic Society, 2019. http://dx.doi.org/10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a1216.
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Robinson, Reeder M., Leticia Reyes, Ravyn Duncan, and Nathan Dolloff. "Abstract 3863: Specifically targeting PDIA1 with indene inhibitors leads to bortezomib-potentiation in multiple myeloma." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-3863.
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Robinson, Reeder M., Leticia Reyes, Ravyn Duncan, and Nathan Dolloff. "Abstract 3863: Specifically targeting PDIA1 with indene inhibitors leads to bortezomib-potentiation in multiple myeloma." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-3863.
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Kumar, A., E. Elko, S. Bruno, Z. Mark, N. Chamberlain, B. Korwin-Mihavics, R. Chandrasekaran, et al. "Ablation or Inhibition of Protein Disulfide Isomerase A3 (PDIA3) in Club Cells Attenuates Osteopontin (SPP1) Production and Lung Fibrosis." In American Thoracic Society 2021 International Conference, May 14-19, 2021 - San Diego, CA. American Thoracic Society, 2021. http://dx.doi.org/10.1164/ajrccm-conference.2021.203.1_meetingabstracts.a4241.
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Miyoshi, Yuji, Seiki Takagi, Shu amiki, and Ken-ichi Kitayama. "Multi period PM OLM with Dynamic Counter Propagating Effects Compensation for 5 bit All optical Analog to digital Conversion." In Optical Fiber Communication Conference. Washington, D.C.: OSA, 2009. http://dx.doi.org/10.1364/ofc.2009.pdpa6.
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Shi, J. W., F. M. Kuo, and M. Z. Chou. "A Linear Cascade Near-Ballistic Uni-Traveling-Carrier Photodiodes with Extremely High Saturation-Current Bandwidth Product (6825mA-GHz, 75mA/91GHz) under a 50Ω Load." In Optical Fiber Communication Conference. Washington, D.C.: OSA, 2010. http://dx.doi.org/10.1364/ofc.2010.pdpa6.
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Yu, Jianjun, Ze Dong, Xin Xiao, Yan Xia, Sheping Shi, Chao Ge, Weiqing Zhou, Nan Chi, and Yufeng Shao. "Generation, Transmission and Coherent Detection of 11.2 Tb/s (112×100Gb/s) Single Source Optical OFDM Superchannel." In Optical Fiber Communication Conference. Washington, D.C.: OSA, 2011. http://dx.doi.org/10.1364/ofc.2011.pdpa6.
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Miyoshi, Yuji, Seiki Takagi, Shu Namiki, and Ken-ichi Kitayama. "Multi-period PM-NOLM with Dynamic Counter-Propagating Effects Compensation for 5-bit All-optical Analog-to-digital Conversion." In National Fiber Optic Engineers Conference. Washington, D.C.: OSA, 2009. http://dx.doi.org/10.1364/nfoec.2009.pdpa6.
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Shi, J. W., F. M. Kuo, and M. Z. Chou. "A Linear Cascade Near-Ballistic Uni-Traveling-Carrier Photodiodes with Extremely High Saturation-Current Bandwidth Product (6825mA-GHz, 75mA/91GHz) under a 50Ω Load." In National Fiber Optic Engineers Conference. Washington, D.C.: OSA, 2010. http://dx.doi.org/10.1364/nfoec.2010.pdpa6.
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Yu, Jianjun, Ze Dong, Xin Xiao, Yan Xia, Sheping Shi, Chao Ge, Weiqing Zhou, Nan Chi, and Yufeng Shao. "Generation, Transmission and Coherent Detection of 11.2 Tb/s (112×100Gb/s) Single Source Optical OFDM Superchannel." In National Fiber Optic Engineers Conference. Washington, D.C.: OSA, 2011. http://dx.doi.org/10.1364/nfoec.2011.pdpa6.
Full textReports on the topic "PDIA6"
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McNaught, Tim. A Problem-Driven Approach to Education Reform: The Story of Sobral in Brazil. Research on Improving Systems of Education (RISE), March 2022. http://dx.doi.org/10.35489/bsg-rise-ri_2022/039.
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For more than two decades, the Brazilian municipality of Sobral has focused intensively on improving the quality of its public education system; the resulting success has been remarkable. In 2005, the Brazilian federal government started calculating a Basic Education Development Index (IDEB in Portuguese), which measures the quality of education in schools across the country. In the inaugural results in 2005, 1,365 municipalities had a better score for primary education than Sobral. By 2017, Sobral made national news by ranking number one in the entire country for both primary and lower secondary education (Cruz and Loureiro, 2020). These results are even more impressive when considering that Sobral is located in the northeastern state of Ceará, which is the fifth poorest state in Brazil in terms of GDP per capita (Cruz and Loureiro, 2020). The case of Sobral exhibits many elements that are similar to Problem Driven Iterative Adaptation (PDIA), an approach wherein problems are key to driving change (Andrews et al., 2015). The PDIA approach relies on reformers to identify problems that matter, break them down into their root causes, identify entry points, act, stop to reflect, and then iterate and adapt their way to a solution.1 This process of constant feedback and experimentation by local actors allows for the development of a solution that fits the local context. This paper explores the transformation of Sobral’s education system through the lens of PDIA2 , with an emphasis on the early reform period of 2000-2004. Many excellent papers have been written, in Portuguese and English, about the case of Sobral; this paper draws heavily on this existing literature.3 The paper is also supported by interviews from key individuals who either were closely involved with the reform efforts or have studied them. The paper follows the narrative of the Sobral story, starting in 1997, and uses boxes and other diagrams to view the reform efforts through the lens of PDIA. Finally, the paper explains how the reform efforts grew and scaled over the years, not only within Sobral, but also to other municipalities in Ceará and across Brazil.
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Richards, Robin. Can PDIA be Used at a Strategic Level in Multi-Donor Trust Funds and Across Various Phases of Downstream Projects? Institute of Development Studies, June 2022. http://dx.doi.org/10.19088/k4d.2022.112.
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This rapid review investigated whether PDIA can be used successfully in Multi-Donor Trust Funds (MDTF) at a strategic level and also across various phases of downstream projects (Verheijen, 2017 and World Bank 2018). The review also identified best practices across the whole programme iteration cycle. The review found that PDIA can be successfully used in MDTF’s and across the various phases of downstream projects. There were few examples illustrating the use the PDIA approach in Multi-Donor Trust Fund interventions. The body of published research on the use of PDIA was relatively small. However, there was more literature on the use of PDIA in large scale interventions generally at system-wide level and lower down, at project level. Literature was mainly from grey sources, including case studies published by organisations; reports from multi-donor projects; university projects; online blogs; and annual reports from development and funding organisations. Sources for the review were quite recent, between the periods 2010-2021.
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Barjum, Daniel. PDIA for Systems Change: Tackling the Learning Crisis in Indonesia. Research on Improving Systems of Education (RISE), September 2022. http://dx.doi.org/10.35489/bsg-rise-ri_2022/046.
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Indonesia is facing a learning crisis. While schooling has increased dramatically in the last 30 years, the quality of education has remained mediocre (Rosser et al., 2022). Teacher capability is an often cited weakness of the system, along with policies and system governance. Approaches focused primarily on adding resources to education have not yielded expected outcomes of increased quality. “It is a tragedy that in the second decade of the twenty-first century, some children in Indonesia are not completing primary school and are turned out into the workforce as functional illiterates.” (Suryadarma and Jones, 2013; Nihayah et al., 2020). In the early 2000s, Indonesia began a process of decentralising service delivery, including education, to the district level. Many responsibilities were transferred from the central government to districts, but some key authorities, such as hiring of civil service teachers, remained with the central government. The Indonesian system is complex and challenging to manage, with more than 300 ethnic groups and networks of authority spread over more than 500 administrative districts (Suryadarma and Jones, 2013). Niken Rarasati and Daniel Suryadarma researchers at SMERU, an Indonesian think tank and NGO, understood this context well. Their prior experience working in the education sector had shown them that improving the quality of education within the classroom required addressing issues at the systems level (Kleden, 2020). Rarasati noted the difference in knowledge between in-classroom teaching and the systems of education: “There are known-technologies, pedagogical theories, practices, etc. for teaching in the classroom. The context [for systems of education] is different for teacher development, recruitment, and student enrollment. Here, there is less known in the public and education sector.” Looking for ways to bring changes to policy implementation and develop capabilities at the district level, SMERU researchers began to apply a new approach they had learned in a free online course offered by the Building State Capability programme at the Center for International Development at Harvard University titled, “The Practice of PDIA: Building Capability by Delivering Results”. The course offered insights on how to implement public policy in complex settings, focused on using Problem Driven Iterative Adaptation (PDIA). The researchers were interested in putting PDIA into practice and seeing if it could be an effective approach for their colleagues in government. This case study reviews Rarasati and Suryadarma’s journey and showcases how they used PDIA to foster relationships between local government and stakeholders, and bring positive changes to the education sector.
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Samji, Salimah, and Mansi Kapoor. Funda Wande through the Lens of PDIA: Showcasing a Flexible and Iterative Learning Approach to Improving Educational Outcomes. Research on Improving Systems of Education (RISE), January 2022. http://dx.doi.org/10.35489/bsg-rise-ri_2022/036.
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Funda Wande has adopted a ‘learning by doing’ strategy that is similar to the Problem Driven Iterative Adaptation (PDIA) approach to solving complex problems. PDIA is a high-impact process of innovation that helps organisations develop the capability to solve complex problems while they are solving such problems. It is a step-by-step framework that helps break down problems into their root causes, identify entry points, search for possible solutions, take action, reflect upon what is learned, adapt, and then act again. Its dynamic process and tight feedback loops enable teams to find and fit solutions to the local context. This case provides a narrative of the Funda Wande story with boxes illustrating how PDIA principles and tools like problem construction, deconstruction, entry point analysis, iteration, and building authorisation would have been applied in practice. The sources of this case include a literature review of education in South Africa, related research documents, and conversations with staff at Funda Wande.