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Dissertations / Theses on the topic 'PcrA'

Author: Grafiati
Published: 4 June 2021
Last updated: 30 January 2023

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1

Chisty, L. T. "PcrA function in plasmid replication." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1427879/.

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PcrA is a DNA helicase involved in unwinding plasmi ds as a part of a complex in asymmetric rolling - circle replication of certain plasmids carrying antibiotic resistance genes. PcrA translocates on single stranded DNA by coupling ATP hydrolysis to movement on DNA. Initiator protein, RepD is required to nick supercoiled plasmid site - specifically and open an ssDNA stretch that PcrA can bind. The presence of RepD is needed throughout plasmid unwinding to maintain processivity. Using fluorescent - based techniques, PcrA helicase mechanistic functions and interactions with different components
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2

Cerqueira, Lucas Saar. "PCRA - um protocolo cooperativo de acesso ao meio para redes de sensores aquáticas." Universidade Federal de Juiz de Fora (UFJF), 2018. https://repositorio.ufjf.br/jspui/handle/ufjf/7214.

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3

Lynch, Gerard Patrick. "Molecular characterization of the interaction between the plasmid initiator protein RepD and PcrA helicase." Thesis, University of Leeds, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.536100.

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4

Dillingham, Mark Simon. "Biochemical studies on DNA helicases." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312245.

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5

Cravello, Laëtitia. "Etudes structurales des protéines par spectrométrie de masse couplée aux échanges hydrogène/deuterium et à la réticulation chimique." Université Joseph Fourier (Grenoble), 2005. https://tel.archives-ouvertes.fr/tel-00009698.

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Les protéines sont impliquées dans de nombreux processus biologiques. Il est nécessaire pour comprendre en détail leur fonction et leur mode d’action afin d’obtenir des informations sur leur structure et sur leurs interactions éventuelles avec leurs partenaires. Le travail réalisé durant cette thèse a consisté à développer deux méthodes innovantes utilisant la spectrométrie de masse pour étudier la structure des protéines et à appliquer ces méthodes à une problématique biologique. Nous avons optimisé une méthode associant les échanges H/D et la spectrométrie de masse sur une protéine modèle, l
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6

Caye-Eude, Aurélie. "Génétique et architecture clonale des leucémies myélomonocytaires juvéniles sporadiques et syndromiques." Thesis, Sorbonne Paris Cité, 2018. http://www.theses.fr/2018USPCC173/document.

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La LMMJ est un syndrome myéloprolifératif et myélodysplasique rare du jeune enfant, initiée par des mutations classiquement décrites comme mutuellement exclusives de RAS (NRAS, KRAS) ou de régulateurs de la voie RAS (PTPN11, NF1 ou CBL). Ces mutations, somatiques ou constitutionnelles, entraînent l’hyperactivation de cette voie de signalisation et une hypersensibilité spécifique au GM-CSF. La LMMJ est une hémopathie sévère dont le seul traitement est l’allogreffe de moelle osseuse. Cependant sa présentation et son évolution sont particulièrement hétérogènes puisqu’une transformation en leucémi
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7

Salgado, Vanessa Riesz. "Desenvolvimento de Reações em Cadeia pela Polimerase (PCRs) para o diagnóstico diferencial das principais espécies de Brucella." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-11102012-115215/.

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A brucelose é uma doença altamente contagiosa, responsável por grandes prejuízos econômicos e de saúde pública. É causada por bactérias do gênero Brucella, cujas espécies e seus biovares costumam ser caracterizados pelo isolamento e identificação de características fenotípicas da colônia. Dificuldades como, o perigo na manipulação dos microrganismos, processos laboriosos de tipificação, demora na obtenção de resultados e a instabilidade de características fenotípicas ou isolamento de linhagens atípicas dificultam a tipificação e encorajaram a busca de técnicas mais sensíveis e específicas, com
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8

Voigt, Kristina. "Einfluss der patientenkontrollierten epiduralen Analgesie versus der patientenkontrollierten intravenösen Analgesie auf immunologische Parameter nach großen Wirbelsäulenoperationen." Doctoral thesis, Humboldt-Universität zu Berlin, Medizinische Fakultät - Universitätsklinikum Charité, 2007. http://dx.doi.org/10.18452/15589.

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Operationen mit großem Gewebetrauma können mit starken postoperativen Schmerzen und ausgeprägten perioperativen Homöostasestörungen einhergehen. Dabei werden sowohl hyperinflammatorische als auch immunparalytische Reaktionen beobachtet, die sich negativ auf den postoperativen Verlauf auswirken können. Um eine effektive und sichere Analgesie zu gewähren, werden alternativ zu der intravenösen Therapie mit Opioiden zunehmend epidurale Verfahren eingesetzt. In dieser prospektiven, randomisierten und doppelblinden Studie wurde die patientenkontrollierte epidurale Analgesie mit der patientenkontroll
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9

Minnella, Walter Settimo Leonardo. "Development of microfluidic tools for biological applications." Thesis, Bordeaux, 2017. http://www.theses.fr/2017BORD0664.

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Cette thèse traite le développement de dispositifs, basés sur la technologie "laboratoire sur puce"(LOC) qui visent à contrôler l'environnement des systèmes biologiques pour des applications macro et microbiologiques. En effet, les caractéristiques de la microfluidique permettent de manipuler l'environnement cellulaire à un niveau supérieur à celui du degré de contrôle atteignable avec les techniques ordinaires. Dans ce travail de thèse sera explorée la possibilité de profiter de ces fonctions afin de développer des outils de diagnostic peu coûteux et pourtant efficaces. En particulier, on rap
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10

Ohlsson, Staffan. "Modellering och styrning av flis till en sulfatkokare." Thesis, Linköping University, Department of Electrical Engineering, 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-2941.

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<p>At the Skoghall pappermill, sulphatepaper pulp is produced in a continuous digester originally from 1969. To be able to maintain a high level of production there is a need for a process with few disturbances. Variations in how well the wooden chips are packed in the digester is one form of disturbance. Today there are no available measurements on how well the chips are packed. Instead this is regarded as being constant. </p><p>The variation in the so called bulk density of the chips is mainly due to variations in the percentage with small dimensions. Chips are classified in relation to thei
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11

Rozales, Franciéli Pedrotti. "Real time-pcr e nested-pcr no diagnóstico da tuberculose pulmonar." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2013. http://hdl.handle.net/10183/72990.

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A tuberculose (TB) é um importante problema de saúde pública, portanto, é necessário o desenvolvimento de novas ferramentas para a detecção rápida e confiável do Mycobacterium tuberculosis, prevenindo a transmissão da TB. O objetivo deste estudo foi avaliar dois testes moleculares para detecção de bactérias do Complexo Mycobacterium tuberculosis (MTBC) diretamente de amostras clínicas. Foi realizado um estudo transversal, no qual foram selecionadas 124 amostras respiratórias. As amostras foram avaliadas por dois testes moleculares “in house” para detecção do MTBC: NESTED-PCR (NPCR) e Real Time
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12

Ziebell, Kim. "Evaluation of PCR and PCR-RFLP protocols to identify the Shiga toxins." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0026/MQ51107.pdf.

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13

SPÍNDULA, FILHO José Vieira de. "Detecção de HPV e avaliação do índice de proliferação celular entre carcinomas espinocelulares e carcinomas verrucosos de boca." Universidade Federal de Goiás, 2006. http://repositorio.bc.ufg.br/tede/handle/tde/1364.

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Made available in DSpace on 2014-07-29T15:21:56Z (GMT). No. of bitstreams: 1 DIS J V S FILHO 2006.pdf: 804840 bytes, checksum: 74be1a5db70d292dc48c7621acb661f3 (MD5) Previous issue date: 2006-11-27<br>Squamous cell carcinoma (SCC) is the most common malignant neoplasm of the bucal cavity, and one of its variants is verrucous carcinoma (VC), of low degree malignancy. The diagnosis of VC is difficult from the clinical as well as from the histopathological point of view, and an effective diagnosis is vital when deciding on the treatment and prognosis of this tumor. The aim of this research was
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14

Hill, Philip John. "PCR based gene engineering." Thesis, University of Nottingham, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317040.

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15

Costa, Luciana Fachini da [UNESP]. "Avaliação comparativa entre PCR gênero-específica, PCR espécie-específica e nested PCR espécie-específica no diagnóstico da infecção por Brucella ovis." Universidade Estadual Paulista (UNESP), 2010. http://hdl.handle.net/11449/94670.

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Made available in DSpace on 2014-06-11T19:27:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-06-29Bitstream added on 2014-06-13T20:08:22Z : No. of bitstreams: 1 costa_lf_me_botfmvz.pdf: 594547 bytes, checksum: 7d609573704d70bd41e6633e86629767 (MD5)<br>Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)<br>Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)<br>O diagnóstico da infecção por Brucella ovis em ovinos usualmente é realizado por meio de exame clínico, testes sorológicos e bacteriologia. Devido às limitações apresentadas pelas técnicas, o diagnós
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16

Costa, Luciana Fachini da. "Avaliação comparativa entre PCR gênero-específica, PCR espécie-específica e nested PCR espécie-específica no diagnóstico da infecção por Brucella ovis /." Botucatu : [s.n.], 2010. http://hdl.handle.net/11449/94670.

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Resumo: O diagnóstico da infecção por Brucella ovis em ovinos usualmente é realizado por meio de exame clínico, testes sorológicos e bacteriologia. Devido às limitações apresentadas pelas técnicas, o diagnóstico é geralmente obtido mediante aplicação de duas ou mais técnicas para obtenção de um resultado conclusivo. A Reação em Cadeia pela Polimerase (PCR) tem sido utilizada como ferramenta diagnóstica aplicável, pois é sensível, pouco dispendiosa, rápida, simples de ser realizada e permite o diagnóstico específico do agente infectante. Neste trabalho foram realizados dois experimentos. No pri
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17

Nyström, Stefan. "Evaluation of a New Method for Extraction of Drift-Stable Information from Electronic Tongue Measurements." Thesis, Linköping University, Department of Electrical Engineering, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-1615.

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<p>This thesis is a part of a project where a new method, the base descriptor approach, is studied. The purpose of this method is to reduce drift and extract vital information from electronic tongue measurements. Reference solutions, called descriptors, are measured and the measurements are used to find base descriptors. A base descriptor is, in this thesis, a regression vector for prediction of the property that the descriptor represent. The property is in this case the concentration of a chemical substance in the descriptor solution. Measurements from test samples, in this case fruit juices,
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18

Jakobsson, Sanna. "Quantitative analysis of BCR-ABL1 fusion gene by Droplet Digital PCR and qRT-PCR." Thesis, Umeå universitet, Biomedicinsk laboratorievetenskap, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-103957.

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19

Metivier, Romain. "Ecologie microbienne de produits végétaux : Adaptation de traitements assainissants pour la valorisation de ces produits." Thesis, Bordeaux, 2015. http://www.theses.fr/2015BORD0419/document.

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L’utilisation de coproduits en tant que matière première provenant d’une autre voie industrielle, fait qu’il n’est plus considéré comme « déchet ». Leur valorisation est donc un axe de développement pour les entreprises agronomiques et agroalimentaires. Cependant, leur nouveau statut de « matière première » entraîne des contraintes pour les industriels.Celles-ci sont diverses selon les voies de destination : sanitaires, toxicologiques …Ce travail s’intéresse à deux coproduits issus de filières différentes de transformation végétale :(1) l’épiderme de pomme, comme source d’antioxydants. Leur va
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20

Ertongur, Sabahat Işin. "Funktionen und Regulation der PCNA-Ubiquitinierung in Vertebraten." kostenfrei, 2009. http://edoc.ub.uni-muenchen.de/11549/.

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Ertongur, Sabahat Isin. "Funktionen und Regulation der PCNA-Ubiquitinierung in Vertebraten." Diss., lmu, 2009. http://nbn-resolving.de/urn:nbn:de:bvb:19-115497.

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Smith, Jane Showler. "PCNA and P53 expression in mouse liver tumours /." [S.l : s.n.], 1995. http://www.ub.unibe.ch/content/bibliotheken_sammlungen/sondersammlungen/dissen_bestellformular/index_ger.html.

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23

Ohayon, Delphine. "Rôle de PCNA cytoplasmique dans la survie cellulaire." Thesis, Sorbonne Paris Cité, 2016. http://www.theses.fr/2016USPCC324.

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Notre laboratoire a mis en évidence la présence de la protéine Proliferating Cell Nuclear Antigen (PCNA) localisée exclusivement dans le cytosol du neutrophile exerçant une activité anti-apoptotique. C'est une protéine dite "scaffolding" qui s'associe à de nombreuses protéines partenaires pour assumer ses fonctions. Dans des conditions physiologiques, la relocalisation du noyau vers le cytosol de PCNA s'effectue à la fin de différenciation granulocytaire. Mon projet de thèse a permis d'identifier que cette relocalisation cytoplasmique était dérégulée dans les cellules leucémiques contribuant a
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24

Saukkoriipi, A. (Annika). "Detection of pneumococcus by PCR." Doctoral thesis, University of Oulu, 2003. http://urn.fi/urn:isbn:9514272110.

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Abstract New rapid methods for sensitive and specific detection of pneumococci are not only needed to improve the diagnosis of pneumococcal disease but are also essential for vaccine and carriage studies. The purpose of this study was to develop sensitive PCR methods for the detection and quantification of S. pneumoniae and to study the applicability of these methods to detecting pneumococci in clinical samples. A previously described PCR method was first developed further by introducing a Europium-labelled hybridisation probe for the detection of amplification products. The hybridisation me
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Beauchamps, Patrick. "Contribution de l'amplification génique (PCR) au diagnostic de la toxoplasmose : intérêts de la PCR quantitative." Lille 1, 1999. https://pepite-depot.univ-lille.fr/LIBRE/Th_Num/1999/50376-1999-413.pdf.

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La toxoplasmose est une parasitose ubiquitaire occupant une large place en médecine humaine et vétérinaire. Cette affection parasitaire très fréquente en France est le plus souvent bénigne voire asymptomatique. Pourtant, elle reste redoutable en situation de transmission congénitale. De même, sa survenue chez les malades immunodéprimés a radicalement changé la conception de cette maladie. Pour compenser les insuffisances du sérodiagnostic et du diagnostic parasitologique, nous avons mis au point un diagnostic permettant un dépistage très précoce face à la suspicion d'une toxoplasmose congénita
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Chandramoulee, Swaran Yuvaneswari. "Evaluation of direct PCR for forensic DNA profiling and the development of a direct PCR multiplex." Thesis, University of Strathclyde, 2012. http://oleg.lib.strath.ac.uk:80/R/?func=dbin-jump-full&object_id=18935.

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The use of direct PCR with different types of sample was explored in this study. Genomic DNA preparations at various concentrations and buccal cell counts were deposited on commonly encountered substrates, recovered and amplified using direct PCR before subjecting them to capillary electrophoresis. The electropherograms obtained were compared to those obtained using the standard DNA profiling protocol which involves extraction and amplification prior to capillary electrophoresis. Direct PCR was found to be better than the standard DNA profiling protocol in both studies and was further tested w
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Moldovan, George-Lucian. "Regulation of replication-linked functions by PCNA and SUMO." Diss., lmu, 2006. http://nbn-resolving.de/urn:nbn:de:bvb:19-63450.

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28

Ola, Ayodele Oluronke. "A functional analysis of proliferating cell nuclear antigen (PCNA)." Thesis, King's College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.391441.

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29

Shetty, Shiphali. "In vivo replication dynamics : analysis of PCNA-interacting proteins." Thesis, University of Cambridge, 2013. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.607734.

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30

Cloux, Boccoz Stéphanie. "Développement de PCRs multiplexes pour le diagnostic : microarrays analytiques." Thesis, Lyon 1, 2015. http://www.theses.fr/2015LYO10282/document.

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Les travaux présentés dans cette thèse font suite à celle de Melle LE GOFF. Ils se concentrent sur la technologie HIFI brevetée et développée pendant ses travaux. Une première partie du travail présenté dans ce manuscrit concerne le test HIFI Blood 96™ et plus particulièrement les améliorations et les évolutions apportées au test afin d'en faire un véritable outil de génotypage, multiparamétrique et haut-débit pouvant être installé dans les banques de sang dans le but de constituer des inventaires de sang génotypé de façon étendue, participant ainsi à améliorer la sécurité transfusionnelle. Il
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Bischoff, Erik. "Schnellnachweis von bierschädlichen Bakterien mit PCR." [S.l.] : [s.n.], 2002. http://deposit.ddb.de/cgi-bin/dokserv?idn=964420333.

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32

Lien, Tonje Gulbrandsen. "Statistical Analysis of Quantitative PCR Data." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for matematiske fag, 2011. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-13094.

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This thesis seeks to develop a better understanding of the analysis of gene expression to find the amount of transcript in a sample. The mainstream method used is called Polymerase Chain Reaction (PCR) and it exploits the DNA's ability to replicate. The comparative CT method estimate the starting fluorescence level f0 by assuming constant amplification in each PCR cycle, and it uses the fluorescence level which has risen above a certain threshold. We present a generalization of this method, where different threshold values can be used. The main aim of this thesis is to evaluate a new method ca
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Karlsson, Magdalena, and Emilia Semberg. "Tracing probiotics in salami using PCR." Thesis, Uppsala universitet, Institutionen för medicinsk biokemi och mikrobiologi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-157177.

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Starter cultures of different bacteria strains like lactic acid producing bacteria, Staphylococcus and Kocuria are used when making salami. Starter cultures give the sausage specific flavours and improve the quality and ripening of the final product. Probiotic strains can also be added during the production of salami. Studies have shown that probiotics are good for health and are therefore added to food, such as fermented sausages. In order to work as a probiotic strain, the bacteria have to survive during the production process, storage and through the whole human gastrointestinal tract. The
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Renkjumnong, Wasuta. "SVD and PCA in Image Processing." Digital Archive @ GSU, 2007. http://digitalarchive.gsu.edu/math_theses/31.

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The Singular Value Decomposition is one of the most useful matrix factorizations in applied linear algebra, the Principal Component Analysis has been called one of the most valuable results of applied linear algebra. How and why principal component analysis is intimately related to the technique of singular value decomposition is shown. Their properties and applications are described. Assumptions behind this techniques as well as possible extensions to overcome these limitations are considered. This understanding leads to the real world applications, in particular, image processing of neurons.
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Halpern, Micah. "Immuno-PCR detection of Lyme borreliosis." Doctoral diss., University of Central Florida, 2013. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/6286.

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Lyme borreliosis, more commonly referred to as Lyme disease, is the fastest growing zoonotic disease in North America with approximately 30,000 confirmed cases and 300,000 estimated infections per year. In nature, the causative agent of Lyme disease, the bacterium Borrelia burgdorferi, cycles between Ixodes sp. ticks and small mammals. Humans become infected with Lyme disease after being bitten by an infected tick. The primary indicator of a Borrelia burgdorferi infection is a bull's eye rash typically followed by flu-like symptoms with treatment consisting of a 2-4 week course of antibiotics.
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Bennett, Marissa A. "Improving Model Performance with Robust PCA." Digital WPI, 2020. https://digitalcommons.wpi.edu/etd-theses/1366.

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As machine learning becomes an increasingly relevant field being incorporated into everyday life, so does the need for consistently high performing models. With these high expectations, along with potentially restrictive data sets, it is crucial to be able to use techniques for machine learning that increase the likelihood of success. Robust Principal Component Analysis (RPCA) not only extracts anomalous data, but also finds correlations among the given features in a data set, in which these correlations can themselves be used as features. By taking a novel approach to utilizing the output fro
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Liu, Peng. "Adaptive Mixture Estimation and Subsampling PCA." Case Western Reserve University School of Graduate Studies / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=case1220644686.

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Feugeas, Olivier. "Pcr (polymerase chain reaction) et vih." Lille 2, 1990. http://www.theses.fr/1990LIL2M264.

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Moravčíková, Simona. "Stanovenie hodnoty firmy PCA Slovakia, s.r.o." Master's thesis, Vysoká škola ekonomická v Praze, 2015. http://www.nusl.cz/ntk/nusl-206699.

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The aim of this thesis is to estimate the value of the company PCA Slovakia, s.r.o. to the 31st December 2015. The thesis is divided into two parts, in the concrete the theoretical and practical parts. The theoretical part describes the basic concepts necessary for the valuation of the company and it is kind of the point for the practical part. On the other hand, the practical part is focused on the introduction of the company and the application of strategic analysis and financial analysis, the prognosis of revenue and other value drivers, financial plan and finally the actual valuation of th
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Šuranská, Hana. "Identifikace vinných kvasinek metodou PCR-RFLP." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2009. http://www.nusl.cz/ntk/nusl-216494.

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This thesis deals with identification of the wine yeasts by applying the PCR-RFLP method. The identification and characteristic of the yeasts has gone through substantial changes in recent years. There have been introduced new methods of taxonomic classifying based on the molecular methods, which are oriented to easy and fast identification. One of these methods is the PCR-RFLP method. The amplification of the 5•8S-ITS rDNA sequence by the polymerase chain reaction with use of the primers ITS1 and ITS4 leads to the amplification of the specific sequence of DNA. Such multiplied DNA is after rep
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Pelikánová, Veronika. "Automatická genotypizace bakterií metodou rep-PCR." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2018. http://www.nusl.cz/ntk/nusl-378029.

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This thesis deals with automatic bacteria genotyping by rep-PCR method. Its theoretical part presents various methods of DNA typing, basic information on electrophoresis and modern electrophoretic approaches, including their problems, misleading data distortion. In order to automate typing, there has been introduced a program for phylogenetic sample classification from rep-PCR, also applicable for data from chip capillary electrophoresis. The program consists of three main parts: digitization, bandmatching and clustering apparatus to bacterial type classification. The result of the algorithm i
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Reis, Levi Eduardo Soares. "Detecção de Leishmania por PCR e suas variações (seminested PCR e PCR em tempo real), em fragmentos de pele e de baço de cães com leishmaniose visceral." Programa de Pós-Graduação em Ciências Farmacêuticas. CIPHARMA, Escola de Farmácia, Universidade Federal de Ouro Preto, 2013. http://www.repositorio.ufop.br/handle/123456789/3364.

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Noll, Lance. "Escherichia coli O157: detection and quantification in cattle feces by quantitative PCR, conventional PCR, and culture methods." Thesis, Kansas State University, 2015. http://hdl.handle.net/2097/18923.

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Master of Science<br>Department of Diagnostic Medicine/Pathobiology<br>T. G. Nagaraja<br>Shiga toxin-producing E. coli O157 is a major foodborne pathogen. The organism colonizes the hindgut of cattle and is shed in the feces, which serves as a source of contamination of food. Generally, cattle shed E. coli O157 at low concentrations (≤ 10[superscript]2 CFU/g), but a subset of cattle, known as “super-shedders”, shed high concentrations (>10[superscript]3 CFU/g) and are responsible for increased transmission between animals and subsequent hide and carcass contamination. Therefore, concentrati
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Lima, Ana Carolina Stocco de. "Diagnóstico molecular de Leishmaniose Tegumentar Americana: identificação de espécies de Leishmania por SSUrDNA PCR e G6PD PCR." Universidade de São Paulo, 2010. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-20092010-162811/.

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A Leishmaniose Tegumentar Americana (LTA) representa um sério problema de saúde pública. No Brasil muitas espécies são reconhecidas como patogênicas para o homem, portanto o diagnóstico diferencial é necessário para compreender o perfil epimemiológico da LTA em áreas endêmicas. Com o objetivo de identificar espécies de Leishmania utilizando ferramentas moleculares, cinquenta e três biópsias de pele de pacientes com LTA, fixadas em formalina e incluídas em parafina dos Estados do Pará (N=33) e Maranhão (20) foram submetidos a diferentes protocolos da Reação em Cadeia da Polimerase (PCR) para a
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45

SPACOV, Isabel Cristina Guerra. "Utilização de marcadores de rDNA-PCR e tDNA-PCR para tipagem de isolados clínicos de Pseudomonas aeruginosa." Universidade Federal de Pernambuco, 2005. https://repositorio.ufpe.br/handle/123456789/6640.

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Made available in DSpace on 2014-06-12T18:06:32Z (GMT). No. of bitstreams: 2 arquivo6313_1.pdf: 1481990 bytes, checksum: 994600fb8e4272b6f803e8477c45a54d (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2005<br>Pseudomonas aeruginosa é uma bacteria Gram-negativa ubíqua e oportunista. Na rotina hospitalar, os marcadores fenotípicos nem sempre revelam a diversidade das bactérias distribuídas nos diversos setores, assim, aplicamos três métodos moleculares baseados na amplificação por PCR do locus de rDNA e tDNA para caracterizar a divers
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Ferreira, Karin Correa Scheffer. "Detecção do vírus da raiva em órgãos de morcegos do gênero Artibeus (Leach, 1821) por meio de RT-PCR, Hemi-Nested RT-PCR e Real Time RT-PCR." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/10/10134/tde-19102012-132944/.

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Este estudo teve como objetivo detectar a presença do vírus da raiva em diferentes órgãos de morcegos do gênero Artibeus empregando as técnicas moleculares como RT-PCR, hnRT-PCR e Real Time RT-PCR. De aproximadamente 4000 espécimes de morcegos recebidas no Instituto Pasteur para o diagnóstico da raiva, foram selecionados 30 morcegos do gênero Artibeus, com resultados positivos para raiva pelas técnicas tradicionais de IFD e inoculação em células N2A utilizando suspensões feitas a partir do SNC. Para as técnicas moleculares, foram retirados glândulas salivares, bexigas urinárias, rins, pulmões
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Pfander, Boris. "Regulation der Genomstabilität durch SUMO- und Ubiquitin-Modifikation von PCNA." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-44792.

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Tomi, Nils-Sebastian. "Funktionelle Analyse der PCNA-Polyubiquitinierung und der E3-Ligase SHPRH." Diss., Ludwig-Maximilians-Universität München, 2015. http://nbn-resolving.de/urn:nbn:de:bvb:19-182348.

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Ludwig, Cornelia. "Structural and biochemical studies of PCNA and its molecular interactions." Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/12483.

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Proliferating cell nuclear antigen (PCNA) plays a key role in DNA replication and repair. In this thesis I have determined the crystal structure of <i>S. pombe </i>PCNA on its own and in complex with a peptide derived from the natural inhibitor of PCNA in humans, p21. Using the p21 peptide as a template, chemical libraries were screened for small drug-like molecules that are able to mimic the protein-protein interaction between human PCNA and p21 and tested for binding using various fluorescence-based methods. Binding of selected compounds could not be observed, however, which may have been du
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Lamb, Vanessa Martins. "Arqueologia hist?rica eg?pcia do per?odo de Amarna." Pontif?cia Universidade Cat?lica do Rio Grande do Sul, 2008. http://tede2.pucrs.br/tede2/handle/tede/2255.

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Made available in DSpace on 2015-04-14T13:46:32Z (GMT). No. of bitstreams: 1 402761.pdf: 455290 bytes, checksum: 11f4b3fba95b413fa82ce5cfadd49fd6 (MD5) Previous issue date: 2008-06-25<br>Com a ascen??o de Akhenaton ao trono egipcio, inicia-se um per?odo que traz in?meras transforma??es ? sociedade do per?odo. Forem objetos de pesquisa as teorias que apontam para uma poss?vel co-reg?ncia entre novo rei e seu pai, Amen?fis III; a substitui??o do antigo pante?o por um ?nico Deus, Aton, e todas as implica??es que essa transforma??o trouxe ? sociedade eg?pcia; al?m da transfer?ncia da capital de
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