Relevant bibliographies by topics / Paratuberculosi

Academic literature on the topic 'Paratuberculosi'

Author: Grafiati
Published: 9 March 2023
Last updated: 11 March 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Contents

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Paratuberculosi.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Paratuberculosi"

1

Laranja-da-Fonseca, Luis Femando, Alexandre Azevedo Oliva, Christian Campos Pereira, and Marcos Veiga dos Santos. "Doença de Johne: uma doença emergente em rebanhos leiteiros brasileiros." Revista de Educação Continuada em Medicina Veterinária e Zootecnia do CRMV-SP 3, no. 2 (July 1, 2000): 30–39. http://dx.doi.org/10.36440/recmvz.v3i2.3336.

Full text
Abstract:
A Paratuberculose ou Doença de Johne é uma doença infecto-contagiosa, causada pelo Mycobacterium paratuberculosis, que se caracteriza por um processo inflamatório granulomatoso no intestino dos ruminantes domésticos e selvagens, determinando redução na digestibilidade dos alimentos, com consequente queda na produção de leite. Os animais infectados geralmente apresentam diarreia progressiva e perda de peso, Ainda que a literatura nacional apresente descrições da ocorrência de paratuberculose como casos isolados, não existem dados sobre a ocorrência da paratuberculose em rebanhos leiteiros no Brasil. Em estudo recente que avaliou a presença de anticorpos anti-M. paratuberculosis em rebanhos leiteiros do Estado de São Paulo, LARANJA-DA-FONSECA et al. (1999) identificaram que dos 403 animais amostrados, 153 (37,9%) apresentaram anticorpos anti-Mycobacterium paratuberculosis e, das 20 fazendas amostradas, 19 (95%) tiveram pelo menos um animal positivo, existindo assim a necessidade de levantamentos epidemiológicos da doença, afim de que se possa avaliar o real impacto da paratuberculose nos rebanhos brasileiros.
APA, Harvard, Vancouver, ISO, and other styles
2

Zavgorodniy, A. I., S. A. Pozmogova, N. V. Goncharova, M. V. Kalashnyk, and V. V. Bilushko. "The study of epizootic sera obtained from ruminant animals in complement fixation test (CFT) with the use of Paratuberculous antigen." Veterinary Medicine: inter-departmental subject scientific collection, no. 105 (August 7, 2019): 41–45. http://dx.doi.org/10.36016/vm-2019-105-8.

Full text
Abstract:
The paper presents results of the study of epizootic blood sera in the complement fixation test (CFT) with paratuberculous antigen. Blood sera were sampled from the cattle and goats. The antigen was produced from the culture filtrate of Mycobacterium avium subspecies paratuberculosis (MAP) in the laboratory for tuberculosis study. The aim of the present study was to clarify the epizootic situation concerning Johne’s disease among the dairy cattle in different regions of Ukraine. To achieve this aim the blood sera from cattle and goats were collected from farms in different regions of Ukraine. Those sera samples were studied in the complement fixation test with the use of paratuberculous antigen that was produced from the culture filtrate of MAP. The above mentioned blood sera were collected from the cattle that had positive allergic reactions on the use of tuberculin (PPD) for mammals. Those animals belonged to the free from tuberculosis and paratuberulosis milk farms. The study of obtained samples of blood sera was conducted in the accordance with the methodological guidelines “Laboratory diagnostics of paratuberculosis” (shutter. NMR FEFU pr. No. 1, dated December 19, 2014). There were studied 1098 blood sera samples from cattle. In addition to this, investigation was conducted on 24 samples of blood sera from goats. As the result of conducted study it was found that 17 samples of blood sera contained specific antibodies against MAP (serum solution 1:10). These blood sera collected from the cattle belonging to 4 farms in Poltava, Donetsk and Khmelnitsky regions. Along with this it was obtained 9 uncertain results in compliment fixation test that was conducted between paratuberculous antigen (ACF) and blood sera from those 4 farms. The results of monitoring studies indicate that M. avium subsp. paratuberculosis pathogen circulates in studied farms. This can lead to the complication of the epizootic situation regarding paratuberculosis and contribute to the spreading of this pathogen to other free from MAP infection farms. There are no anti-paratuberculosis antibodies in blood serum from goats. It is necessary to conduct annual monitoring serological studies of productive dairy cattle and imported animals in order to clarify and control epizootic situation concerning paratuberculosis on the territory of Ukraine
APA, Harvard, Vancouver, ISO, and other styles
3

Konté, M. "La paratuberculose. Diagnostic d'un premier cas chez un bovin d'importation au Sénégal." Revue d’élevage et de médecine vétérinaire des pays tropicaux 41, no. 2 (February 1, 1988): 147–48. http://dx.doi.org/10.19182/remvt.8714.

Full text
Abstract:
Le diagnostic de paratuberculose est porté pour la première fois au Sénégal sur un bovin importé de France. Les circonstances d’isolement de Mycobacterium paratuberculosis sont rapportées. Les particularités culturales du germe sont évoquées et discutées. L’auteur conclut en invoquant la nécessité d’appliquer les mesures de police sanitaire arrêtées en la matière.
APA, Harvard, Vancouver, ISO, and other styles
4

Mota, Rinaldo A., Paulo V. Peixoto, Elise M. Yamasaki, Elizabeth S. de Medeiros, Mateus M. da Costa, Rodolfo M. Peixoto, and Marilene F. Brito. "Ocorrência de paratuberculose em búfalos (Bubalus bubalis) em Pernambuco." Pesquisa Veterinária Brasileira 30, no. 3 (March 2010): 237–42. http://dx.doi.org/10.1590/s0100-736x2010000300008.

Full text
Abstract:
A paratuberculose (doença de Johne) é uma das doenças de maior importância econômica para ruminantes em vários países e pode representar uma ameaça ao desenvolvimento da pecuária brasileira. É uma doença infecto-contagiosa que provoca enterocolite granulomatosa crônica, incurável e de difícil controle, cujo agente é o Mycobacterium avium subsp. paratuberculosis (MAP). Descreve-se a ocorrência de paratuberculose em um rebanho de búfalos no Estado de Pernambuco, Brasil. Não foi encontrado registro, na literatura, da ocorrência de paratuberculose em búfalos no país. De 100 búfalos, cinco mostravam sinais clínicos característicos da doença. À necropsia de dois animais as lesões estavam restritas ao intestino delgado com evidente espessamento da mucosa, aumento de linfonodos mesentéricos e vasos linfáticos proeminentes e dilatados. À microscopia, observaram-se na mucosa do intestino, infiltrado inflamatório granulomatoso com numerosos macrófagos epitelióides e células gigantes de Langhans, além de bacilos álcool-ácido resistentes (BAAR) visualizados através da coloração de Ziehl-Neelsen (ZN). Nos linfonodos mesentéricos, havia espessamento da cápsula e marcada inflamação granulomatosa. O exame direto pela técnica de ZN para pesquisa do bacilo em esfregaços de fezes, raspado de mucosa intestinal e imprint de linfonodos mesentéricos resultou positivo. A PCR IS900 específico de linfonodo mesentérico e mucosa intestinal revelou amplificação de um fragmento de aproximadamente 110pb, confirmada pela comparação com outras sequências de M. avium subsp. paratuberculosis disponíveis no GenBank.
APA, Harvard, Vancouver, ISO, and other styles
5

Lázaro Sales, Mariana, Érica Bravo Sales, Andréa Alencar Padilha, Omara Tereza Vianello Pereira, and Antônio Augusto Fonseca Junior. "Desenvolvimento de uma PCR em tempo real para diagnóstico de Mycobacterium avium subespécie paratuberculosis." Revista Acadêmica Ciência Animal 11, no. 1 (January 15, 2013): 97. http://dx.doi.org/10.7213/academica.7760.

Full text
Abstract:
A paratuberculose, ou Doença de Johne, é uma doença crônica degenerativa que incide nos ruminantes domésticos. O agente etiológico Mycobacterium avium subsp. paratuberculosis (MAP) é um bacilo de crescimento lento pertencente ao complexo Mycobacterium avium (MAI). Nos EUA, a enfermidade acarreta grandes prejuízos econômicos. No Brasil, são poucos os relatos de casos da doença e não se conhece a real situação epidemiológica da paratuberculose bovina. O objetivo desse trabalho foi padronizar um teste de Reação em Cadeia da Polimerase (PCR) em tempo real para auxiliar na confirmação de casos da enfermidade.A técnica foi testada com DNA de diversas espécies do gênero Mycobacterium e avaliada quanto à sensibilidade e eficiência. Uma vez atestada sua confiabilidade, a metodologia foi aplicada para confirmar um caso suspeito de paratuberculose bovina. Foi enviada para análise no Laboratório Nacional Agropecuário/MG (Lanagro/MG) amostra de linfonodo mesentérico e alça intestinal de um bovino da raça holandesa com suspeita de paratuberculose. A amostra foi processada e inoculada para crescimento em meio Herrold’s com micobactina. A combinação entre a técnica do isolamento bacteriano em meio apropriado juntamente com a PCR em tempo real foi capaz de diagnosticar a presença da micobactéria no animal. Esse foi o primeiro caso de paratuberculose confirmado em um laboratório oficial do Ministério da Agricultura.
APA, Harvard, Vancouver, ISO, and other styles
6

Schwarz, D. G. G., I. A. Carvalho, P. A. G. Pietralonga, A. C. S. Faria, and M. A. S. Moreira. "Paratuberculose em pequenos ruminantes domésticos." Arquivos do Instituto Biológico 79, no. 3 (September 2012): 443–52. http://dx.doi.org/10.1590/s1808-16572012000300019.

Full text
Abstract:
Mycobacterium avium subesp. paratuberculosis (MAP) é o agente etiológico da paratuberculose em ruminantes domésticos e silvestres. Em caprinos e ovinos causa uma enterite granulomatosa crônica com emagrecimento progressivo seguido de morte, raramente a diarreia é observada. Nesse aspecto, a doença é considerada uma ameaça mundial aos rebanhos, pois pode permanecer no estádio subclínico por anos, manifestando perdas indiretas na produção animal e na disseminação do agente. Além disso, existem relatos da possível relação de MAP com a doença de Crohn, determinando, assim, a sua relevância na saúde pública. O Brasil não dispõe de dados quantificando as reais perdas produtivas nos rebanhos acometidos pela doença, e poucas informações do comprometimento de caprinos e ovinos no país são relatadas. Assim, este artigo busca revisar a paratuberculose em pequenos ruminantes domésticos.
APA, Harvard, Vancouver, ISO, and other styles
7

Yamasaki, Elise M., Carlos H. Tokarnia, Alexandre Galvão, Marcos J. P. Gomes, José A. B. Chies, Tiago Degani Veit, Ana Paula Aragão, and Marilene F. Brito. "Aspectos clínico-patológicos e controle da paratuberculose em rebanho bovino leiteiro." Pesquisa Veterinária Brasileira 30, no. 11 (November 2010): 921–32. http://dx.doi.org/10.1590/s0100-736x2010001100005.

Full text
Abstract:
A paratuberculose ou doença de Johne é uma enterite granulomatosa causada por Mycobacterium avium subsp. paratuberculosis. Descrevem-se os aspectos epidemiológicos, clínico-patológicos e laboratoriais da paratuberculose em rebanho bovino leiteiro no município de Rio Claro, região Sul do Estado do Rio de Janeiro. No período de 2006 a 2009, oito vacas adultas da raça Girolanda apresentaram diarreia crônico-intermitente e perda progressiva de peso. À necropsia, observaram-se linfonodos mesentéricos aumentados de volume e úmidos ao corte, vasos linfáticos subserosos das alças intestinais proeminentes, serosa do intestino com aspecto anelado e cerebroide e a mucosa espessada, pregueada e com aspecto microgranular. À microscopia havia, desde o duodeno até o reto, inflamação granulomatosa difusa, marcada dilatação dos vasos linfáticos no ápice das vilosidades, linfangiectasia e linfangite granulomatosa na submucosa, muscular e serosa. A inflamação granulomatosa também foi vista nos linfonodos mesentéricos. A coloração de Ziehl-Neelsen revelou variável quantidade de bacilos álcool-ácido resistentes no interior de macrófagos, de células gigantes de Langhans e livres na mucosa e submucosa dos intestinos delgado e grosso e em linfonodos mesentéricos. Em alguns animais, a lâmina própria da mucosa, principalmente do jejuno e íleo exibia acentuada hipertrofia. Mycobacterium avium subsp. paratuberculosis foi isolado em cultivo bacteriano de Herrold com micobactina, a partir de amostras de fezes, de raspado de mucosa intestinal e de leite e identificado pela técnica de PCR IS900. Através da avaliação sorológica semestral, foram analisadas 298 vacas do mesmo rebanho a partir de três anos de idade, observou-se cerca de 40% de animais reagentes ao teste ELISA indireto no período estudado. O diagnóstico da paratuberculose foi baseado nos dados clínico-patológicos, na sorologia, no isolamento e identificação do agente através de cultivo bacteriano e PCR IS900. Após implementação de medidas de controle, tais como eliminação de animais doentes, abate seletivo dos animais soropositivos, separação dos bezerros ao nascer e utilização de banco de colostro, observou-se, nos três anos de estudo, diminuição da ocorrência de casos clínicos no rebanho, de seis casos por ano para cerca de um caso por ano.
APA, Harvard, Vancouver, ISO, and other styles
8

Da Silva Filho, Givaldo Bom, Hisadora Advincula Da Silva Chaves, Lorena D’Andrade Aires, Thaiza Campelo Braga, Danielle Pimentel Ribeiro, Huber Rizzo, Lázaro Manoel De Camargo, et al. "Aspectos clínicos e patológicos da paratuberculose em um rebanho de bovinos na Zona da Mata de Pernambuco." Medicina Veterinária (UFRPE) 11, no. 4 (May 7, 2018): 247. http://dx.doi.org/10.26605/medvet-n4-1960.

Full text
Abstract:
A paratuberculose é uma doença infecciosa causada por Mycobacterium avium subsp. paratuberculosis (Map), um bacilo álcool-ácido resistente (BAAR), que se replica nos macrófagos da lâmina própria do intestino delgado e grosso, e que acomete principalmente ruminantes domésticos e selvagens. Os sinais clínicos observados cursam com enterite granulomatosa e crônica. Devido à má absorção de nutrientes, os animais afetados apresentam perda progressiva de peso, mau estado corporal e óbito. Objetivou-se descrever os aspectos clínicos e patológicos de casos de paratuberculose no município de Carpina, Zona da Mata de Pernambuco. Dois bovinos mestiços com holandês foram examinados clinicamente e necropsiados. Os principais sinais clínicos consistiram em pelos quebradiços e sem brilho, perda progressiva de peso, desidratação e diarreia. À necropsia observou-se os linfonodos mesentéricos aumentados de volume, esbranquiçados e edemaciados. No intestino delgado as lesões consistiram em espessamento da mucosa intestinal, principalmente da prega ileocecal. Na avaliação histológica visibilizou-se acentuado infiltrado inflamatório granulomatoso difuso na mucosa intestinal com numerosos macrófagos com aspecto epitelióide, células gigantes de Langhans, além de bacilos álcool-ácido resistentes (BAAR) visualizados através da coloração de Ziehl-Neelsen (ZN). Este trabalho demonstra a ocorrência de casos de paratuberculose no município de Carpina, na Zona da Mata de Pernambuco.
APA, Harvard, Vancouver, ISO, and other styles
9

Yamasaki, Elise M., Marilene de Farias Brito, Douglas McIntosh, Alexandre Galvão, Tiago C. Peixoto, and Carlos Hubinger Tokarnia. "Diagnóstico imuno-histopatológico da paratuberculose subclínica em bovinos no estado do Rio de Janeiro." Pesquisa Veterinária Brasileira 33, no. 12 (December 2013): 1427–32. http://dx.doi.org/10.1590/s0100-736x2013001200006.

Full text
Abstract:
O diagnóstico precoce e específico da paratuberculose ainda é um desafio. Isto pode estar associado à baixa sensibilidade dos testes laboratoriais e ou à variação da resposta imunológica frente à infecção por Mycobacterium avium subsp. paratuberculosis. Mundialmente, é uma enfermidade que causa importantes prejuízos econômicos, em especial, à bovinocultura leiteira, devido ao caráter crônico da infecção. No Brasil, a paratuberculose já foi descrita em diversas espécies de ruminantes domésticos e em vários estados, o que demonstra que a enfermidade está presente em território nacional e há a necessidade de elaboração de técnicas de diagnóstico para a confirmação da infecção. O objetivo deste trabalho foi caracterizar os achados anátomo-histopatológicos e imuno-histoquímicos em intestino e linfonodos mesentéricos de bovinos assintomáticos, provenientes de rebanhos positivos para paratuberculose localizados no estado do Rio de Janeiro, Brasil. O estudo macroscópico revelou alterações inespecíficas tais como áreas avermelhadas na mucosa do intestino, aumento do volume das placas de Peyer e dos linfonodos mesentéricos, além disso, observou-se que vasos linfáticos mesentéricos estavam dilatados e esbranquiçados. Do total de 52 vacas leiteiras avaliadas, a histopatologia revelou infiltração granulomatosa, por vezes com formação de células gigantes multinucleadas, em mucosa e ou submucosa de jejuno, íleo e em linfonodos mesentéricos, principalmente na região cortical, em 32 animais. Estes bovinos foram submetidos à coloração de Ziehl-Neelsen cujo teste não demonstrou reação positiva, no entanto, quando analisados pelo teste imunohistoquímico para Mycobacterium spp. observou-se imunorreação em 6 animais. Desta forma, a histopatologia e imunohistoquímica pode ser uma importante ferramenta para diagnóstico da paratuberculose subclínica.
APA, Harvard, Vancouver, ISO, and other styles
10

Yamasaki, Elise M., Marilene F. Brito, Rinaldo A. Mota, Douglas McIntosh, and Carlos H. Tokarnia. "Paratuberculose em ruminantes no Brasil." Pesquisa Veterinária Brasileira 33, no. 2 (February 2013): 127–40. http://dx.doi.org/10.1590/s0100-736x2013000200001.

Full text
Abstract:
A paratuberculose ou doença de Johne é uma enterite granulomatosa causada por Mycobacterium avium subsp. paratuberculosis (Map) e comumente afeta ruminantes domésticos, no entanto, pode infectar várias espécies de mamíferos. Está presente nos cinco continentes e é considerada endêmica em algumas regiões pela Organização Internacional de Epizootias (OIE). Pertence à lista de enfermidades notificáveis, que compreende as doenças transmissíveis de importância sócio-econômica e/ou em saúde-pública, cujo controle é necessário para o comércio internacional de animais e alimentos de origem animal. A importância da doença de Johne não se restringe somente aos prejuízos econômicos causados à indústria animal, mas também na possível participação do Map na íleocolite granulomatosa que afeta seres humanos, conhecida como doença de Crohn. No Brasil, a paratuberculose já foi descrita em diversas espécies de ruminantes e em vários estados. Embora os relatos naturais da enfermidade sejam pontuais, acredita-se na possibilidade da transmissão interespecífica e na disseminação do agente através da compra e venda de animais infectados. O objetivo deste artigo foi reunir as informações disponíveis referentes aos aspectos epidemiológicos, clínico-patológicos e laboratoriais da paratuberculose em bovinos, bubalinos, caprinos e ovinos no Brasil, e salientar a necessidade de implementação de medidas de controle sanitário da enfermidade no país, o que possibilitaria a melhoria da qualidade e valorização dos produtos de origem animal no mercado internacional.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Paratuberculosi"

1

Chastel, Michaël Foucras Gilles. "Épidémiologie de la paratuberculose des ruminants conséquences sur les mesures de contrôle et de prévention /." [S.l.] : [s.n.], 2008. http://oatao.univ-toulouse.fr/2081/1/debouch_2081.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Marcé, Clara. "Modelling the transmission of and effectiveness of control measures for Mycobacterium avium subsp. Paratuberculosis in dairy herds." Rennes 1, 2010. http://www.theses.fr/2010REN1S047.

Full text
Abstract:
Paratuberculosis is a worldwide incurable disease of ruminants resulting in a decrease in milk production and slaughter value. The aim of this thesis was to evaluate the epidemiological and economic effectiveness of selected control programmes in infected dairy herds. A stochastic simulation model has been developed to represent both the population dynamics within a dairy herd and the transmission of Mycobacterium avium subsp. Paratuberculosis (Map). It has been coupled to an existing bioeconomic model. The spontaneous within-herd progression of Map infection after the introduction of one infected cattle in an initially susceptible herd was studied in the absence of control measure. The effect of within-herd contacts on Map spread in a persistently infected herd was investigated. The cost-effectiveness of test-and-cull strategies to control Map infection in dairy herds was assessed. Simulation outcomes put forward that, even when no control measure is implemented, fadeout can occur if less than two clinically affected animals are present. In persistently infected herds, the two main transmission routes are transmission via the environment of the farm and in utero transmission. Isolating calves from their herd mates during the first weeks of age has no significant impact on Map transmission. Limiting or delaying calf exposure to adult faeces and early culling of clinically affected adults are thus recommended to decrease Map prevalence in infected dairy herds. Systematic test-and-cull appears cost-effective if implemented from the day one infected cattle is introduced. The tool designed here is flexible and enables studying other control options within a dairy herd
La paratuberculose est une maladie incurable des ruminants entraînant une diminution de la production laitière et de la valeur de carcasse des animaux infectés. L'objectif de ma thèse est d'évaluer l'efficacité épidémiologique et économique d'actions de maîtrise en troupeaux bovins laitiers infectés. Un modèle de simulation stochastique représentant la dynamique de population d'un troupeau bovin laitier et la transmission indirecte de Mycobacterium avium subsp. Paratuberculosis (Map) a été, élaboré puis couplé à un simulateur bioéconomique existant. L'évolution spontanée de l'infection après I'introduction d'un animal infecté dans un troupeau initialement sensible est étudiée en l'absence d'action de maîtrise. L'effet de la structure de contact sur la transmission de Map est exploré. La rentabilité de stratégies de maîtrise de l'infection est évaluée. Une extinction de l'infection peut survenir lorsque moins de deux animaux cliniquement infectés sont présents en cinq ans, en l'absence d'action de maîtrise. Dans les troupeaux infectés persistants, la transmission in utero et via l'environnement contaminé sont les deux principales voies de transmission. Empêcher le contact précoce entre veaux n'a pas d'impact sur la transmission de Map. Il est recommandé de limiter ou retarder l'exposition des veaux aux fèces des adultes et de réformer rapidement les animaux cliniquement infectés pour réduire la prévalence de l'infection. L'utilisation de tests suivis de réformes mis en place systématiquement dès l'introduction d'un animal infecté semble rentable. L'outil développé ici est flexible et permettra d'étudier d'autres actions de maîtrise en troupeau laitier
APA, Harvard, Vancouver, ISO, and other styles
3

Matado, André Filipe Fernandes. "Seroprevalência de paratuberculose em ovinos e caprinos na região do Baixo Alentejo." Master's thesis, Universidade de Évora, 2011. http://hdl.handle.net/10174/15722.

Full text
Abstract:
No presente trabalho verificámos se existia circulação do agente da paratuberculose nas explorações de pequenos ruminantes no distrito de Beja. Avaliou-se a variação da circulação do agente em função das raças, localização geográfica, coabitação com outras espécies e dimensão das explorações. O estudo consistiu na pesquisa serológica que incidiu em 27 explorações de regime semi-extensivo utilizando um teste ELISA em animais com idade superior a 2 anos. Foram analisadas 25 explorações de ovinos de aptidão carne e duas de caprinos. Foram rastreados no total 187 animais, 172 ovinos e 15 caprinos. A seroprevalência aparente de rebanho encontrada foi 14,8%, com intervalo de confiança 5,9 a 32,5%. Na estimativa da seroprevalência real de rebanho este valor sobe para 41,2%, com intervalo de confiança 16,4 a 90,2%. Apenas o factor coabitação dos rebanhos rastreados com rebanhos de bovinos revelou ter significância, podendo ser considerado factor de risco nos rebanhos de pequenos ruminantes; ABSTRACT: In this study we tested the circulation of Paratuberculosis in small ruminant flocks in the district of Beja. We evaluated the variation of the agent circulation with race, geographic location, cohabitation with other species and flocks size. The study consisted of a serological survey in 27 semi-extensive farms using an ELISA test in animals older than 2 years. We surveyed25 sheep flocks designed for meat production and two goats flocks. We screened a total of 187 animals, 172 sheep and 15 goats. The apparent herd prevalence found was 14.8% with a confidence limit from 5.9 to 32.5%. In estimating the real herd prevalence this figure rises to 41.2% with a confidence limit from 16.4 to 90.2%. Only the cohabitation flocks factor with cattle flocks seems to be significant and can be considered a risk factor in small ruminants.
APA, Harvard, Vancouver, ISO, and other styles
4

PIGOLI, CLAUDIO. "MYCOBACTERIAL DISEASES IN VETERINARY MEDICINE: MORPHOPATHOLOGY AND MYCOBACTERIAL PHENOTYPES." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/843675.

Full text
Abstract:
Mycobacteria are an ancient bacterial taxon that has, over time, had a profound impact on mankind and domesticated animals. Mycobacteria are, in fact, responsible for significant human and animal diseases whose severe economic and public health repercussions make them still highly topical today. The first topic of research in this Thesis is a widely characterized phenomenon in the context of human tuberculosis, but one which has, to date, been little considered in animal tubercular infections, namely mycobacterial dormancy. As bovine tuberculosis (bTB) is undoubtedly the most significant of the animal tubercular infections, these aspects have been investigated precisely in the context of this pathology. In particular, this study aimed to verify the intralesional existence of non-acid-fast (non-AF) mycobacteria phenotypes that in human medicine have been associated to the phenomenon of mycobacterial dormancy. Another important objective of this study was to develop a histological method capable of detecting in the same section the mycobacterial cells by combining AF stains and techniques capable of highlighting the mycobacteria regardless of their AF features. In this retrospective study, 250 bovine lymph nodes (LNs) in which Mycobacterium bovis (Mb) has been identified by the diagnostic algorithm routinely applied by the Italian National Reference Center for bTB, were randomly enrolled. Ziehl-Neelsen (ZN) stained sections were evaluated for each sample. Only LNs with at least one granuloma containing more than 20 ZN-positive bacilli were selected, and the corresponding formalin-fixed and paraffin-embedded blocks were retrieved. For each block, a 4-µm thick section was obtained and, after reducing the autofluorescence through a photobleaching protocol and unmasking the Mb antigens with enzymatic digestion, the sections were submitted to a protocol combining Auramine O stain and an indirect immunofluorescence assay targeting Mb antigens; a DAPI-containing mounting medium was used. Processed slides were viewed with a Leica DM6 B upright microscope, and the obtained images were elaborated with a deconvolution algorithm. Of the 250 enrolled LNs, 24 contained at least one granuloma with more than 20 ZN-positive bacilli, and in all of them, AF and non-AF bacilli were identified. In all (24/24) selected LNs, the non-AF bacillary load resulted greater than the AF one, especially in the central parts of the granuloma; non-AF bacilli were also identified within the cytoplasm of multinucleated giant macrophages (MGMs). This study revealed for the first-time non-AF Mb phenotypes within bovine lymph nodal granulomatous lesions. It also demonstrated that a widely studied pathology such as bTB still poses new issues regarding pathogenesis and host-pathogen interaction and suggested that bTB can be considered an effective animal model for studying mycobacterial dormancy. The second study carried out was also focused on a tubercular infection but, in this latter case, both the mycobacterial species involved and the affected host were different. Indeed, in the second study, wild boar (WB) M. microti (Mm) infection, whose epidemiological and pathogenic dynamics are still largely unknown, was enquired into. Specifically, the natural WB Mm infection was investigated by evaluating the granulomatous lesions' histological features and Mm microbiological isolation. For this purpose, 103 WB retropharyngeal and submandibular LNs in which Mm was identified by gyrB restriction fragment length polymorphism PCR were retrospectively selected and histologically assessed. For each sample, Hematoxylin-eosin and ZN-stained slides were evaluated. Considered histological variables were the number of granulomas, size and maturational stage of granulomas, number of MGMs, and AF bacilli per granuloma. Furthermore, Mm microbiological results were also considered. Investigated parameters were statistically analyzed. Mm microbiological isolation was negatively influenced by granulomas maturation and positively affected by AF bacilli's presence within the section. Granuloma maturation was positively influenced by granuloma size and negatively affected by the number of granulomas in the section and the number of MGMs within the granuloma. The obtained results indicate that granulomas' maturation ensured an efficient containment of Mm infection in the WB, making the intraspecific transmission of the disease an unlikely event. Finally, an outbreak of paratuberculosis in a group of scimitar-horned oryxes (SHOs) kept in a zoological park, gave us the chance to describe various aspects of this disease in an endangered animal species subject to an international conservation and reintroduction plan encompassing several countries. In particular, after the death of six of the 10 SHOs, serial investigations of dead and alive animals were performed. Necropsy, carried out on five out of six animals, identified intestinal thickening and mesenteric lymphadenomegaly in one of the animals. Histopathology (5/6) revealed lepromatous (2/5) and tuberculoid (2/5) intestinal forms or lack of lesions (1/5). ZN and immunohistochemistry stains identified two multibacillary, two paucibacillary forms, and one negative case. M. avium subsp. paratuberculosis (Map) was identified by quantitative PCR (qPCR) in tissue samples in five out of five SHOs and was microbiologically isolated from two of the three animals whose fresh tissue samples were available. Fecal samples were collected in four of the six dead animals: all four resulted positive to qPCR and Map was isolated in three. ELISA identified Map-specific antibodies in three of the five dead animals whose serum was available. qPCR identified Map in the freshly deposited feces of two out of the four alive animals. From the feces of these two animals, Map was microbiologically isolated in one case. All isolates were classified as Map type C and profiled as INMV2 and MVS27 by molecular analysis. Genomic analysis of a field isolate revealed clusterization with a European clade but was more similar to Italian than East European isolates. Our findings highlight again that paratuberculosis should always be considered in zoological parks where endangered species are hosted. Infection can be subclinical, and multiple combined testing techniques may be necessary. The studies included in this Thesis made the examination of certain aspects of significant animal mycobacterial diseases possible, and the results obtained demonstrate that a multidisciplinary approach is the best option when studying infectious diseases, and this is especially true in case of infections deserving to be managed in a One Health perspective.
APA, Harvard, Vancouver, ISO, and other styles
5

Gomes, Marcos José Pereira. "Aspectos epidemiológicos da paratuberculose bovina no Rio Grande do Sul." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2002. http://hdl.handle.net/10183/3448.

Full text
Abstract:
Descrevem-se os achados clínicos e patológicos da paratuberculose em uma criação intensiva de bovinos de leite no municí pio de Capela de Santana, RS. Os sinais clínicos foram observados em oito de um total de 345 bovinos, consistindo em diarréia crônica refratária aos tratamentos, emagrecimento progressivo e queda na produção de leite. As principais lesões macroscópicas, observadas nos oito animais necropsiados, incluíam intestino delgado com acentuado espessamento da parede e superfície mucosa de aspecto reticulado, semelhante às circunvoluções cerebrais, lesão essa perceptível, através da serosa. A luz intestinal estava preenchida com conteúdo fluido e de aspecto leitoso. Os vasos linfáticos do mesentério mostravam-se mais evidentes sendo que alguns tinham aspecto varicoso. Os linfonodos mesentéricos estavam aumentados de volume e, ao corte, fluía grande quantidade de líquido leitoso. Focos de mineralização foram observados na íntima das artérias, nas válvulas cardíacas e na serosa do rúmen. As principais lesões macroscópicas incluíam enterite, linfadenite e linfangite granulomatosa caracterizada por infiltrado inflamatório com macrófagos, células gigantes de Langhans que continham grande quantidade de bacilos álcool-ácido-resistentes. As lesões vasculares consistiam em degeneração e mineralização das túnicas í ntimas e média das artérias de grande calibre associada a proliferação de colágeno. Havia calcificação da serosa do rúmen atrofia hepatocelular difusa e hepatite granulomatosa multifocal. O M. avium subsp. paratuberculosis (Map) foi isolado em amostras de intestino e linfonodos de 8 vacas Holandesas (3,5%) com doença de Johne, dentre 229 amostras cultivadas provenientes de um rebanho leiteiro. Amostras inoculadas em HEYM com micobactina produziram colônias identificadas como Map, segundo as caracterí sticas fenotí picas próprias como: crescimento lento, coloração álcool-ácido-resistente (A.A.R.) e dependência a micobactina. O laboratório de Referência da OIE confirmou a amostra isolada. O M. avium subsp. paratuberculosis (Map) foi isolado em amostras de intestino e linfonodos de 8 vacas Holandesas (3,5%) com doença de Johne, dentre 229 amostras cultivadas provenientes de um rebanho leiteiro Amostras inoculadas em HEYM com micobactina produziram colônias identificadas como Map, segundo as caracterí sticas fenotí picas próprias como: crescimento lento, coloração álcool-ácido-resistente (A.A.R.) e dependência a micobactina. O laboratório de Referência da OIE confirmou a amostra isolada. Não houve isolamento do agente em 221 amostras intestinais quando processadas, após 2 anos de sua colheita. O teste de IDGA aplicado como “screening”, detectou 26 vacas (11,4%) positivas, dentre 228 animais testados e sacrificados em matadouro. O teste de ELISA adsorvido, utilizando o antí geno PPA-3 detectou 125 (39,8%) amostras positivas. O ELISA não adsorvido detectou mais 32 (10,1%) reagentes positivos, dentre os 314 bovinos testados. A prevalência da infecção causada pelo Map em 36 rebanhos leiteiros procedentes de 25 municí pios do Rio Grande do Sul foi estimada em 44,6% das 1316 amostras testadas. A infecção foi identificada em 35 (97,2%) dos 36 rebanhos testados e presentes em todos os municí pios incluí dos. A ocorrência da doença de Johne foi enfatizada, tanto a forma clí nica quanto a infecção subclí nica no Rio Grande do Sul, sugerindo a adoção de medidas de controle sejam aplicadas na proteção dos rebanhos leiteiros nacionais.
The clinical and pathological aspects of bovine paratuberculosis in a dairy herd in the county of Capela de Santana, Rio Grande do Sul, southern Brazil are described. Clinical signs in eight cows out 345 cattle included chronic diarrhea refractory to treatment, progressive emaciation and decreased milk production. Necropsy findings included severe and diffuse thickening of the mucosa of the small intestine with exaggerated reticular pattern, reflected in the serosal surface, similar to cerebral gyri. Intestinal loops were filled with whitish watery contents. The lymphatic vessels of the intestinal serosa and of the mesentery were thickened and tortuous. The mesenteric lymph nodes were enlarged and, on cut surface, there were oozing of milky fluid. Plaques of mineralization were evident in the intima of larger arteries, in the endocardium of the heart valves and in the ruminal serosa. There was also edema of abomasal folds and atrophy of caudate lobe of the liver. The main histopathological findings were granulomatous enteritis, lymphadenitis and lymphangitis. The inflammatory infiltrate was composed of macrophages, epithelioid cells and Langhan`s giant cells containing large numbers of acid-fast bacilli. Vascular lesions included degeneration, calcification and collagen proliferation of the intima and media of larger arteries. Other microscopic lesions included calcification of the ruminal serosa, diffuse hepatocellular atrophy and multifocal granulomatous hepatitis. M. avium subsp. paratuberculosis (Map) was isolated from intestines and lymph nodes samples obtained from 8 Holstein cows (3.5%) with Johne’s Disease among 229 inoculated samples from a unique dairy herd in Rio Grande do Sul State, South Brazil. Cultures on HEYM supplemented with mycobactin yielded colonies identified like Map by phenotypic properties: Slow growth, acid-fast stain and mycobactin dependency. OIE Reference Laboratory in Argentine confirmed the strain like Map. The attempts to isolate the agent from 221 intestinal samples processed after 2 years were unsuccessful. The AGIDT applied as a screening test detected 26 positive cows (11.4%) among 228 tested animals at slaughterhouse. The absorbed ELISA test using PPA-3 antigen detected 125 positive animals (39.8%) and 47 (14.9%) suspect samples among 314 animals tested. Nonabsorbed ELISA test detected more 32 (10.1%) positive animals and 18 (5.7%) suspect samples than absorbed ELISA test. The prevalence of infection caused by Map was estimated in 35 bovine dairy herds in Rio Grande do Sul State by absorbed ELISA using PPA-3 commercial antigen. The ELISA test identified 558 positive (44.6%) animals among 1316 samples tested. The infection was detected in 35 dairy herds (97.2%) among 36 tested and was present in all 25 countries performed. The infection by Map was disseminated in dairy herds in Rio Grande do Sul State. It’ s emphasized the occurrence of clinical and subclinical forms of Johne’ s disease in Rio Grande do Sul and should be necessity the adoption of control measures in the protection of National dairy herds.
APA, Harvard, Vancouver, ISO, and other styles
6

Mathevon, Yoann. "Utilisation des analyses en mélanges pour l'évaluation et le suivi du statut sanitaire des troupeaux : application à la paratuberculose des ovins." Thesis, Toulouse 3, 2018. http://www.theses.fr/2018TOU30044/document.

Full text
Abstract:
La paratuberculose est une maladie enzootique contagieuse des ruminants due à Mycobacterium avium ssp. paratuberculosis (Map). La longue période d'incubation et les faibles performances diagnostiques des tests limitent l'efficacité des plans de maîtrise. Les grands effectifs des troupeaux ovins limitent l'approche par dépistage individuel exhaustif, et les plans de maîtrise s'orientent vers la vaccination, dont les effets n'ont pas été évalués dans les troupeaux français. Á partir d'échantillons de sérum et de fèces de près de 4000 brebis issues de 30 élevages du Lot, les performances diagnostiques de deux trousses sérologiques ELISA et d'une qPCR sur fèces ont été estimées par des modèles à classes latentes bayésiens et fréquentistes. Nos résultats confirment la faible sensibilité et le défaut de spécificité des sérologies ELISA pour la détection des ovins infectés ; la qPCR présentant de meilleures performances diagnostiques. Par ailleurs nous avons évalué les performances diagnostiques relatives des ELISA et de la qPCR appliquées à des mélanges d'échantillons. Dans les deux cas les animaux forts répondeurs étaient détectés de façon systématique, les animaux faiblement positifs étant détectés de façon moins constante. Sur la base de simulations, nous avons évalué les performances des stratégies de dépistage et de suivi basées sur les analyses de mélanges d'échantillons à l'échelle des troupeaux. Alors que la sérologie ELISA est apparue insuffisamment spécifique, l'analyse de mélange de fèces par qPCR semble être une approche prometteuse, permettant de détecter des faibles prévalences d'infection. Enfin les travaux menés dans les troupeaux vaccinés ont précisé dans quelles mesures leur situation épidémiologique pouvait être approchée par l'emploi d'analyses en mélanges
Paratuberculosis is a contagious enzootic disease in ruminants caused byMycobacteriumavium ssp. paratuberculosis (Map). The long incubation period and the low informative values of antemortem diagnostic tests limit the effectiveness of control plans. In large sheep flocks, exhaustive individual testing is unfeasible and control plansmainly focus on vaccination, the effects of which have not yet been evaluated in French flocks. Using blood and feces samples from nearly 4000 ewes in 30 sheep flocks from the French department of Lot, the diagnostic performances of two serum ELISA and one fecal qPCR kits were estimated using bayesian and frequentist latent class modeling. Our results confirm the low sensitivity and non-perfect specificity of serum ELISA for the detection of subclinically infected animals, while the diagnostic performances of fecal qPCR were better. We also evaluated the relative diagnostic performances of pooled-sample analysis for both tests. Highly qPCR/ELISA positive samples were invariously detected while low positive ones were associated with lower detection rates. The flock-level epidemiological performances of screening strategies based on pooled-sample analysis were evaluated by simulation studies. Pooled serum ELISA appeared lowly specific. Conversely, pooled fecal qPCR appeared promising, allowing the detection of low infection prevalence. Finally, the work carried out in the vaccinated flocks made it possible to better know their epidemiological status and to specify to what extent it could be approached using pooled-sample analysis
APA, Harvard, Vancouver, ISO, and other styles
7

Dudemaine, Pier-Luc. "Étude de l'état immunitaire des vaches laitière atteintes de la paratuberculose bovine." Mémoire, Université de Sherbrooke, 2013. http://hdl.handle.net/11143/6298.

Full text
Abstract:
La paratuberculose bovine, ou maladie de Johne, est une maladie inflammatoire intestinale chronique provoquant d’importantes pertes économiques chez les producteurs de ruminants du monde entier. Que ce soit chez la vache laitière ou de boucherie, ces pertes sont causées majoritairement par une diminution de la capacité de reproduction, la baisse de production laitière et l’amaigrissement des vaches qui perdent ainsi beaucoup de valeur à l’abattage, en plus d’être sujettes à une réforme précoce. Outre les pertes économiques, le potentiel de transmission à l’humain est un facteur non négligeable en plus d’un risque de contamination de la chaîne alimentaire. Cette maladie est causée par une bactérie intracellulaire obligatoire nommée Mycobacterium avium subspecies paratuberculosis (MAP). II n’existe actuellement aucune stratégie efficace pour combattre l’infection chez les animaux atteints. L’évolution lente de la maladie fait en sorte que les signes cliniques apparaissent tardivement, soit plusieurs années (4 à 7 ans) après l’infection initiale. Au cours de cette progression, les animaux infectés commencent à excréter le pathogène dans leur environnement. Les animaux atteints deviennent infectieux et peuvent contaminer d’autres congénères, ainsi que leur propre veau. Afin de permettre aux producteurs d’éliminer les vaches atteintes avant qu’elles n’atteignent ce stade, il s’avère important d’établir un diagnostic précoce. Actuellement, ce n’est qu’en phase sous-clinique avancée que les tests diagnostiques sont plus sensibles, soit 2 à 3 ans après le début des excrétions fécales chez les animaux infectés. L'incompréhension du manque de sensibilité des tests de dépistage et de l'évolution de cette maladie justifient les efforts de recherche dans ce domaine en vue de mieux comprendre les réponses immunitaires impliquées dans cette maladie. En effet, une meilleure connaissance des processus d’inflammation chronique pourrait aider à développer des outils diagnostiques complémentaires. Nos résultats suggèrent une dérégulation de la réponse immunitaire. Ainsi, en étudiant les composantes et caractéristiques du sang provenant de vaches infectées, il nous a été possible d’observer que les niveaux de cytokines plasmatiques telles l’interleukine 17 et l'ostéopontine se trouvent sécrétées à différents niveaux chez les vaches atteintes de paratuberculose bovine. De plus, l'analyse de la capacité de leur sérum à soutenir efficacement la prolifération des cellules mononucléées du sang périphérique révèle que le sérum de vaches infectées interfère pour atténuer la prolifération cellulaire. II semble qu’un constituant du sérum provoque une diminution de la réponse immunitaire chez les vaches malades. Les résultats offrnt une appréciation des dérèglements immunitaires provoqués par la paratuberculose bovine.
APA, Harvard, Vancouver, ISO, and other styles
8

Cruz, Catarina Lourenço da. "Paratuberculose: associação dos dados clínicos com os dados de rejeição de carcaças de bovino no matadouro por caquexia." Master's thesis, Universidade de Évora, 2015. http://hdl.handle.net/10174/14002.

Full text
Abstract:
O presente relatório refere-se às atividades no estágio curricular do Mestrado Integrado em Medicina Veterinária. Apresentam-se as atividades no âmbito de sanidade animal, clínica de espécies pecuárias e inspeção sanitária. No âmbito da sanidade animal foram acompanhados os procedimentos do plano de erradicação da brucelose nos Açores. Foram ainda acompanhados casos clínicos de várias áreas de intervenção, principalmente em bovinos de leite. A componente de inspeção sanitária foi desenvolvida no Matadouro de São Miguel, onde foram observados e praticados os procedimentos de inspeção sanitária. Como tema de desenvolvimento optou-se pela paratuberculose (PT) bovina que se manifesta pelo emagrecimento progressivo e diarreia crónica. Colheram-se amostras de sangue, junção ileocecal e fezes a 116 bovinos que apresentavam sinais clínicos compatíveis com PT, sendo que 66,38% dos animais tiveram uma das análises (ELISA ou histopatologia) positivas; ABSTRACT: :“Bovine paratuberculosis association between clinical data and rejection data in slaughterhouse for cachexia.” This document describes the activities during the curricular traineeship of the Master Degree in Veterinary Medicine. Activities carried out on animal health, food ani-mal clinic and meat inspection are presented in this report. In relation to animal health the procedures from the plan of eradication of brucel-losis in Azores as well as the clinical cases from several areas of intervention, especially in dairy cattle were followed. The procedures for meat inspection were learned at São Miguel Slaughterhouse. The subject chosen was bovine paratuberculosis (PT). This disease is manifested by progressive weight loss e chronic diarrhea. In the present study 116 cattle samples (blood, ileo-ceacal junction a feces) were collected from animals with clinical signs com-patible with PT. The results show that 66,38% had one of the tests (ELISA or histopathol-ogy) positive. More studies must be carried out to acknowledge the disease behavior in São Mi-guel Isle. Mais estudos devem ser feitos a fim de averiguar o comportamento da doença na ilha de São Miguel
APA, Harvard, Vancouver, ISO, and other styles
9

Creignou-Mercier, Pascale. "Bases épidémiologiques pour la maîtrise de la paratuberculose caprine." Rennes 1, 2010. http://www.theses.fr/2010REN1S110.

Full text
Abstract:
L’objectif était de produire des connaissances épidémiologiques descriptives pour la conception ultérieure de plans de maîtrise de l’infection des caprins par Mycobacterium avium subspecies paratuberculosis. Une enquête sérologique a permis de confirmer que l’infection par Map était très répandue dans les troupeaux caprins français, avec une prévalence vraie estimée à 63%. La détection des animaux infectés peut être réalisée par un test ELISA, dont la sensibilité et la spécificité ont été estimées à respectivement 53 et 100%. Mais les qualités des tests varient en fonction de l’âge des animaux et les meilleures valeurs ont été obtenues pour les tests ELISA sur des animaux de 2-3 ans. Pour la détection des animaux excréteurs, la PCR est le test le plus précoce (à partir de 8 mois). Comparé à la détection des animaux par PCR, le test de dosage de l’IFNγ a une sensibilité estimée à 49% et le test ELISA a une sensibilité estimée à 29%. Les apports et limites des résultats produits sont discutés dans la perspective de l’élaboration ultérieure d’actions de maîtrise et des perspectives de recherche complémentaires sont avancées
This study aimed at providing descriptive epidemiological knowledge about the infection of goats by Mycobacterium avium subspecies paratuberculosis, in order to design control schemes. A serological study confirmed that Map infection was widespread in goat herds in France (true prevalence estimated to 63%). Detection of infected animals can be made by ELISA, with a sensitivity of 53% and a specificity of 100%. Best values for ELISA tests were obtained for goats aged 2-3 years. For detection of infectious animals, PCR was the earliest test (from 8 months of age). With PCR as reference test, sensitivities for IFNγ test and ELISA test were estimated to 49 and 29%, respectively. Specificities for the 2 tests were estimated to 100%. Lastly, results are discussed in the perspective of the implementation of infection control schemes and further research topics are proposed
APA, Harvard, Vancouver, ISO, and other styles
10

Lefrançois, Louise. "Etude des adhésines HBHA et LBP impliquées dans l'interaction de Mycobacterium avium ssp. paratuberculosis avec les cellules épithéliales intestinales, cibles privilégiées de la bactérie in vivo." Thesis, Tours, 2012. http://www.theses.fr/2012TOUR4020/document.

Full text
Abstract:
Mycobacterium avium ssp. paratuberculosis (Map), agent étiologique de la paratuberculose, a évolué en deuxtypes dénommés, S pour« Sheep » et C pour « Cattle ». L’intestin grêle est le site primaire de l’infection à Map mais les mécanismes moléculaires impliqués dans l’implantation du bacille restent largement méconnus. L’objectif de mon projet de thèse visait à identifier et caractériser les adhésines exprimées par Map par des approches génétiques et biochimiques. J’ai ainsi purifié la HBHA et la LBP par chromatographie d’affinité puis les ai identifiés en spectrométrie de masse. L’originalité de ce travail repose sur le polymorphisme de ces adhésines observé entre les souches de type C et S. Cette variabilité a été mise en évidence sur le domaine d’interaction avec les sucres sulfatés de la cellule hôte influençant l’affinité des adhésines pour l’héparine. Ce travail de thèse a permis de caractériser pour la première fois ces deux adhésines produites par Map. Le polymorphisme de la HBHA et de la LBP, discriminant les types C et S, ouvre de nombreuses perspectives sur l’évolution de l’espèce M. avium et le rôle de ces adhésines sur le tropisme intestinal, la préférence d’hôte de Map ou encore leur potentiel diagnostic
Mycobacterium avium subsp. paratuberculosis (Map), the etiological agent of paratuberculosis, has evolved into two types called, S for "Sheep" and C for "Cattle." The small intestine is the primary site of Map infection but the molecular mechanisms involved in the establishment of bacilli are still unknown. The aim of my thesis was to identify and characterize the adhesins expressed by Map by genetic and biochemical approaches. I purified HBHA and LBP by affinity chromatography then identified them by mass spectrometry. The originality of this work is based on the polymorphism of these adhesins observed between strains of type C and S. This variability has been demonstrated in the binding domain involved in interaction with sulfated sugars of host cell influences adhesins affinity for heparin. This thesis has characterized for the first time these two adhesins produced by Map. Specific polymorphism highlighted related to the evolution of the species avium, opens large number questions on their role on the pathogenesis of Map including the cellular tropism, host preference or interest of these antigens to improve diagnostic
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "Paratuberculosi"

1

W, Sweeney Raymond, ed. Paratuberculosis (Johne's disease). Philadelphia, PA: W.B. Saunders Co., 1996.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Behr, Marcel A., Karen Stevenson, and Vivek Kapur, eds. Paratuberculosis: organism, disease, control. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243413.0000.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Behr, M. A., and D. M. Collins, eds. Paratuberculosis: organism, disease, control. Wallingford: CABI, 2010. http://dx.doi.org/10.1079/9781845936136.0000.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Behr, Marcel A. Paratuberculosis: Organism, disease, control. Wallingford, UK: CABI, 2010.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

International Colloquium on Paratuberculosis (7th 2002 Bilbao, Spain). Proceedings of the Seventh International Colloquium on Paratuberculosis. Madison, WI: International Association for Paratuberculosis, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

International Colloquium on Paratuberculosis (3rd 1991 Orlando, Fla.). Proceedings of the Third International Colloquium on Paratuberculosis: September 28 - October 2, 1991 : a meeting of the International Association for Paratuberculosis, the Orlando Marriott International Drive, Orlando, Florida, U.S.A. Providence, RI: The Association, 1992.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

International Colloquium on Paratuberculosis (5th 1996 Madison, Wis.). Proceedings of the fifth International Colloquium on Paratuberculosis, September 29-October 4, 1996: A meeting of the International Associaiton for Paratuberculosis, Madison, Wisconsin, U.S.A. Rehoboth, MA: International Association for Paratuberculosis, 1997.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

International Colloquium on Paratuberculosis (4th 1994 Cambridge, England). Proceedings of the Fourth International Colloquium on Paratuberculosis, July 17-July 21, 1994: A meeting of the International Association for Paratuberculosis, St. John's College, Cambridge, U.K. Rehoboth, MA: International Association for Paratuberculosis, 1995.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

International Colloquium on Paratuberculosis (8th 2005 Copenhagen, Denmark). Proceedings of the 8th International Colloquium on Paratuberculosis, August 14-18, 2005, Copenhagen, Denmark: A meeting of the International Association for Paratuberculosis, Inc. Madison, WI: International Association for Paratuberculosis, 2005.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

International Colloquium on Paratuberculosis (8th 2005 Copenhagen, Denmark). Proceedings of the 8th International Colloquium on Paratuberculosis, August 14-18, 2005, Copenhagen, Denmark: A meeting of the International Association for Paratuberculosis, Inc. Madison, WI: International Association for Paratuberculosis, 2005.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "Paratuberculosi"

1

Gilmour, N. J. L. "Paratuberculosis." In The Management and Health of Farmed Deer, 113–19. Dordrecht: Springer Netherlands, 1988. http://dx.doi.org/10.1007/978-94-009-1325-7_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Anderson, Daniel C., and Harold M. McClure. "Paratuberculosis, Nonhuman Primates." In Nonhuman Primates I, 148–54. Berlin, Heidelberg: Springer Berlin Heidelberg, 1993. http://dx.doi.org/10.1007/978-3-642-84906-0_23.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Nyange, J. F. C., N. J. L. Gilmour, D. Buxton, and Hugh W. Reid. "Paratuberculosis in Deer." In The Biology of Deer, 151. New York, NY: Springer New York, 1992. http://dx.doi.org/10.1007/978-1-4612-2782-3_37.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Fecteau, Marie-Eve. "Paratuberculosis in cattle." In Paratuberculosis: organism, disease, control, 149–59. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243413.0149.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Begg, Douglas, and Richard Whittington. "Paratuberculosis in sheep." In Paratuberculosis: organism, disease, control, 160–73. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243413.0160.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Lybeck, Kari R., Girum T. Tessema, Annette H. Kampen, Berit Djønne, and Angelika Agdestein. "Paratuberculosis in goats." In Paratuberculosis: organism, disease, control, 174–87. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243413.0174.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Donat, Karsten, Susanne Eisenberg, and Richard Whittington. "Paratuberculosis control measures." In Paratuberculosis: organism, disease, control, 346–64. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243413.0346.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Hussein, Mansour F. "Paratuberculosis (Johne’s Disease)." In Infectious Diseases of Dromedary Camels, 167–73. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-79389-0_27.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Duffy, Shannon C., and Marcel A. Behr. "Paratuberculosis and crohn's disease." In Paratuberculosis: organism, disease, control, 29–44. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243413.0029.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Juste, Ramón A., Joseba M. Garrido, Natalia Elguezabal, and Iker A. Sevilla. "Paratuberculosis vaccines and vaccination." In Paratuberculosis: organism, disease, control, 365–79. Wallingford: CABI, 2020. http://dx.doi.org/10.1079/9781789243413.0365.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Paratuberculosi"

1

Martcheva, M., Michail D. Todorov, and Christo I. Christov. "An Immuno-epidemiological Model of Paratuberculosis." In APPLICATION OF MATHEMATICS IN TECHNICAL AND NATURAL SCIENCES: 3rd International Conference—AMiTaNS'11. AIP, 2011. http://dx.doi.org/10.1063/1.3659918.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Jiang, Xiu-Yun, Chun-Fang Wang, Fan-Li Zeng, Yu-Qing Hu, Xin-Yu Liu, Hao-Ran Ning, and Zhao-Yang He. "Cloning and Expression of 34KDa Protein Gene of Mycobacterium paratuberculosis in Escherichia coli." In 2010 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2010. http://dx.doi.org/10.1109/icbbe.2010.5515315.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Liu, Xinyu, Chunfang Wang, Fanli Zeng, Lei Liu, Xiaoai Zhu, Yanhong Dong, Xiuyun Jiang, et al. "Notice of Retraction: Prokaryotic Expression and Analysis on the 22kDa Protein Gene of Mycobacterium paratuberculosis." In 2011 5th International Conference on Bioinformatics and Biomedical Engineering. IEEE, 2011. http://dx.doi.org/10.1109/icbbe.2011.5780043.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Sanchez, M. P., T. Tribout, S. Fritz, R. Guatteo, C. Fourichon, L. Schibler, A. Delafosse, and D. Boichard. "165. A single step genetic evaluation including causal candidate SNPs for resistance to paratuberculosis in Holstein cattle." In World Congress on Genetics Applied to Livestock Production. The Netherlands: Wageningen Academic Publishers, 2022. http://dx.doi.org/10.3920/978-90-8686-940-4_165.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Knobloch, Henri, Heike Köhler, Nicola Commander, Petra Reinhold, Claire Turner, Mark Chambers, Matteo Pardo, and Giorgio Sberveglieri. "Volatile Organic Compound (VOC) Analysis For Disease Detection: Proof Of Principle For Field Studies Detecting Paratuberculosis And Brucellosis." In OLFACTION AND ELECTRONIC NOSE: Proceedings of the 13th International Symposium on Olfaction and Electronic Nose. AIP, 2009. http://dx.doi.org/10.1063/1.3156505.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Seehusen, F., and S. Scherrer. "Ein neuer S-Stamm von Mycobacterium avium subsp. paratuberculosis bei Ziegen in Graubünden – eine morphologische und molekulare Charakterisierung." In 62. Jahrestagung der Fachgruppe Pathologie der Deutschen Veterinärmedizinischen Gesellschaft. Georg Thieme Verlag KG, 2019. http://dx.doi.org/10.1055/s-0039-1688584.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Usai, M. G., S. Casu, T. Sechi, M. G. Cancedda, D. Pintus, S. Salaris, G. Mulas, and A. Carta. "166. Genetic parameters and QTL mapping for antibody response to paratuberculosis in a natural infected flock of sheep." In World Congress on Genetics Applied to Livestock Production. The Netherlands: Wageningen Academic Publishers, 2022. http://dx.doi.org/10.3920/978-90-8686-940-4_166.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Casu, S., M. G. Usai, T. Sechi, C. Maestrale, S. Miari, S. Salaris, C. Ligios, and A. Carta. "256. Functional and association analyses of sequence data in a region of OAR 20 associated to paratuberculosis in sheep." In World Congress on Genetics Applied to Livestock Production. The Netherlands: Wageningen Academic Publishers, 2022. http://dx.doi.org/10.3920/978-90-8686-940-4_256.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Becher, Gunther, Roman Purkhart, Werner Schüler, Heike Köhler, and Petra Reinhold. "Highly specific differentiation ofmycobacterium avium subspec. paratuberculosis(MAP) in comparison to other mycobacteria using GC IMS-spectral analysis from headspace." In Annual Congress 2015. European Respiratory Society, 2015. http://dx.doi.org/10.1183/13993003.congress-2015.oa3488.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "Paratuberculosi"

1

Bonovska, Magdalena, Tanya Savova, Reneta Petrova, Violeta Valcheva, and Hristo Najdenski. Cases of Paratuberculosis in Deer in Bulgaria. "Prof. Marin Drinov" Publishing House of Bulgarian Academy of Sciences, March 2019. http://dx.doi.org/10.7546/crabs.2019.03.18.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Bercovier, Herve, Michael Collins, Aliza Cohen, and Louis Levy. Recognition and Production of Specific Antigens of Mycobacterium Paratuberculosis. United States Department of Agriculture, October 1992. http://dx.doi.org/10.32747/1992.7600056.bard.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Bercovier, Herve, Raul Barletta, and Shlomo Sela. Characterization and Immunogenicity of Mycobacterium paratuberculosis Secreted and Cellular Proteins. United States Department of Agriculture, January 1996. http://dx.doi.org/10.32747/1996.7573078.bard.

Full text
Abstract:
Our long-term goal is to develop an efficient acellular vaccine against paratuberculosis based on protein antigen(s). A prerequisite to achieve this goal is to analyze and characterize Mycobacterium paratuberculosis (Mpt) secreted and cellular proteins eliciting a protective immune response. In the context of this general objective, we proposed to identify, clone, produce, and characterize: the Mpt 85B antigen and other Mpt immunoreactive secreted proteins, the Mpt L7/L12 ribosomal protein and other immunoreactive cellular proteins, Mpt protein determinants involved in invasion of epithelial cells, and Mpt protein antigens specifically expressed in macrophages. Paratuberculosis is still a very serious problem in Israel and in the USA. In the USA, a recent survey evaluated that 21.6% of the dairy herd were infected with Mpt resulting in 200-250 million dollars in annual losses. Very little is known on the virulence factors and on protective antigens of Mpt. At present, the only means of controlling this disease are culling or vaccination. The current vaccines do not allow a clear differentiation between infected and vaccinated animals. Our long-term goal is to develop an efficient acellular paratuberculosis vaccine based on Mpt protein antigen(s) compatible with diagnostic tests. To achieve this goal it is necessary to analyze and characterize secreted and cellular proteins candidate for such a vaccine. Representative Mpt libraries (shuttle plasmid and phage) were constructed and used to study Mpt genes and gene products described below and will be made available to other research groups. In addition, two approaches were performed which did not yield the expected results. Mav or Mpt DNA genes that confer upon Msg or E. coli the ability to invade and/or survive within HEp-2 cells were not identified. Likewise, we were unable to characterize the 34-39 kDa induced secreted proteins induced by stress factors due to technical difficulties inherent to the complexity of the media needed to support substantial M. pt growth. We identified, isolated, sequenced five Mpt proteins and expressed four of them as recombinant proteins that allowed the study of their immunological properties in sensitized mice. The AphC protein, found to be up regulated by low iron environment, and the SOD protein are both involved in protecting mycobacteria against damage and killing by reactive oxygen (Sod) and nitrogen (AhpC) intermediates, the main bactericidal mechanisms of phagocytic cells. SOD and L7/L12 ribosomal proteins are structural proteins constitutively expressed. 85B and CFP20 are both secreted proteins. SOD, L7/L12, 85B and CFP20 were shown to induce a Th1 response in immunized mice whereas AphC was shown by others to have a similar activity. These proteins did not interfere with the DTH reaction of naturally infected cows. Cellular immunity provides protection in mycobacterial infections, therefore molecules inducing cellular immunity and preferentially a Th1 pathway will be the best candidate for the development of an acellular vaccine. The proteins characterized in this grant that induce a cell-mediated immunity and seem compatible with diagnostic tests, are good candidates for the construction of a future acellular vaccine.
APA, Harvard, Vancouver, ISO, and other styles
4

Karcher, Elizabeth L., Donald C. Beitz, and Judith R. Stabel. Osteopontin Expression in Periparturient Dairy Cows Naturally Infected with Mycobacterium Avium Subsp. Paratuberculosis. Ames (Iowa): Iowa State University, January 2008. http://dx.doi.org/10.31274/ans_air-180814-843.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Barkan, Daniel, Yung-Fu Chang, Patrick McDonough, Susan Fubini, and Robin Gleed. Identification of virulence-associated genes in Mycobacterium avium subsp. paratuberculosis by mutant-library construction. United States Department of Agriculture, January 2016. http://dx.doi.org/10.32747/2016.7600046.bard.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Shpigel, Nahum, Raul Barletta, Ilan Rosenshine, and Marcelo Chaffer. Identification and characterization of Mycobacterium paratuberculosis virulence genes expressed in vivo by negative selection. United States Department of Agriculture, January 2004. http://dx.doi.org/10.32747/2004.7696510.bard.

Full text
Abstract:
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiological agent of a severe inflammatory bowel disease (IBD) in ruminants, known as Johne’s disease or paratuberculosis. Johne’s disease is considered to be one of the most serious diseases affecting dairy cattle both in Israel and worldwide. Heavy economic losses are incurred by dairy farmers due to the severe effect of subclinical infection on milk production, fertility, lower disease resistance and early culling. Its influence in the United States alone is staggering, causing an estimated loss of $1.5 billion to the agriculture industry every year. Isolation of MAP from intestinal tissue and blood of Crohn's patients has lead to concern that it plays a potential pathogenic role in promoting human IDB including Crohn’s disease. There is great concern following the identification of the organism in animal products and shedding of the organism to the environment by subclinically infected animals. Little is known about the molecular basis for MAP virulence. The goal of the original proposed research was to identify MAP genes that are required for the critical stage of initial infection and colonization of ruminants’ intestine by MAP. We proposed to develop and use signature tag mutagenesis (STM) screen to find MAP genes that are specifically required for survival in ruminants upon experimental infection. This research projected was approved as one-year feasibility study to prove the ability of the research team to establish the animal model for mutant screening and alternative in-vitro cell systems. In Israel, neonatal goat kids were repeatedly inoculated with either one of the following organisms; MAP K-10 strain and three transposon mutants of K-10 which were produced and screened by the US PI. Six months after the commencement of inoculation we have necropsied the goats and taken multiple tissue samples from the jejunum, ileum and mesenteric lymph nodes. Both PCR and histopathology analysis indicated on efficient MAP colonization of all the inoculated animals. We have established several systems in the Israeli PI’s laboratory; these include using IS900 PCR for the identification of MAP and using HSP65-based PCR for the differentiation between MAV and MAP. We used Southern blot analysis for the differentiation among transposon mutants of K-10. In addition the Israeli PI has set up a panel of in-vitro screening systems for MAP mutants. These include assays to test adhesion, phagocytosis and survival of MAP to/within macrophages, assays that determine the rate of MAPinduced apoptosis of macrophages and MAP-induced NO production by macrophages, and assays testing the interference with T cell ã Interferon production and T cell proliferation by MAP infected macrophages (macrophage studies were done in BoMac and RAW cell lines, mouse peritoneal macrophages and bovine peripheral blood monocytes derived macrophages, respectively). All partners involved in this project feel that we are currently on track with this novel, highly challenging and ambitious research project. We have managed to establish the above described research systems that will clearly enable us to achieve the original proposed scientific objectives. We have proven ourselves as excellent collaborative groups with very high levels of complementary expertise. The Israeli groups were very fortunate to work with the US group and in a very short time period to master numerous techniques in the field of Mycobacterium research. The Israeli group has proven its ability to run this complicated animal model. This research, if continued, may elucidate new and basic aspects related to the pathogenesis MAP. In addition the work may identify new targets for vaccine and drug development. Considering the possibility that MAP might be a cause of human Crohn’s disease, better understanding of virulence mechanisms of this organism might also be of public health interest as well.
APA, Harvard, Vancouver, ISO, and other styles
7

Bradner, Laura K., Judith R. Stabel, Donald C. Beitz, and Suelee Robbe-Austerman. Shedding of Mycobacterium avium subsp. paratuberculosis into Milk and Colostrum of Naturally Infected Dairy Cows over Complete Lactation Cycles. Ames (Iowa): Iowa State University, January 2013. http://dx.doi.org/10.31274/ans_air-180814-118.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Karcher, Elizabeth L., Donald C. Beitz, and Judith R. Stabel. Parturition Invokes Changes in Peripheral Blood Mononuclear Cell Populations in Holstein Dairy Cows Naturally Infected with Mycobacterium Avium Subsp. Paratuberculosis. Ames (Iowa): Iowa State University, January 2008. http://dx.doi.org/10.31274/ans_air-180814-26.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Osman, Mohamed, Judith Stabel, Jesse M. Hostetter, Daniel S. Nettleton, and Donald C. Beitz. Probiotic Lactobacillus acidophilus strain NP51® Curtails the Progression of Mycobacterium avium Subspecies paratuberculosis (MAP) Infection in Balb/c mice. Ames (Iowa): Iowa State University, January 2011. http://dx.doi.org/10.31274/ans_air-180814-66.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Bradner, Laura, Judith R. Stabel, Donald C. Beitz, and Suelee Robbe-Austerman. Optimization of Methods for the Detection of Mycobacterium avium subsp. paratuberculosis in Milk and Colostrum of Naturally Infected Dairy Cows. Ames (Iowa): Iowa State University, January 2012. http://dx.doi.org/10.31274/ans_air-180814-701.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Paratuberculosi' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography