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1

Peng, Kou-Cheng, Kun-Che Chang, Shyue-Chu Ke, JunRu Chen, and Shu-Ying Liu. "Novel protein photocleavers: Chrysophanol and pachybasin." Journal of Photochemistry and Photobiology 3-4 (September 2020): 100008. http://dx.doi.org/10.1016/j.jpap.2020.100008.

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2

Cadelis, Melissa M., Hugo Gordon, Alex Grey, Soeren Geese, Daniel R. Mulholland, Bevan S. Weir, Brent R. Copp, and Siouxsie Wiles. "Isolation of a Novel Polyketide from Neodidymelliopsis sp." Molecules 26, no. 11 (May 27, 2021): 3235. http://dx.doi.org/10.3390/molecules26113235.

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Fungi have become an invaluable source of bioactive natural products, with more than 5 million species of fungi spanning the globe. Fractionation of crude extract of Neodidymelliopsis sp., led to the isolation of a novel polyketide, (2Z)-cillifuranone (1) and five previously reported natural products, (2E)-cillifuranone (2), taiwapyrone (3), xylariolide D (4), pachybasin (5), and N-(5-hydroxypentyl)acetamide (6). It was discovered that (2Z)-cillifuranone (1) was particularly sensitive to ambient temperature and light resulting in isomerisation to (2E)-cillifuranone (2). Structure elucidation of all the natural products were conducted by NMR spectroscopic techniques. The antimicrobial activity of 2, 3, and 5 were evaluated against a variety of bacterial and fungal pathogens. A sodium [1-13C] acetate labelling study was conducted on Neodidymelliopsis sp. and confirmed that pachybasin is biosynthesised through the acetate polyketide pathway.
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3

Lin, Yi-Ruu, Kou-Cheng Peng, Ming-Huan Chan, Huan-Lin Peng, and Shu-Ying Liu. "Effect of Pachybasin on General Toxicity and Developmental Toxicity in Vivo." Journal of Agricultural and Food Chemistry 65, no. 48 (November 20, 2017): 10489–94. http://dx.doi.org/10.1021/acs.jafc.7b03879.

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4

Lin, Yi-Ruu, Chaur-Tsuen Lo, Shu-Ying Liu, and Kou-Cheng Peng. "Involvement of Pachybasin and Emodin in Self-Regulation ofTrichoderma harzianumMycoparasitic Coiling." Journal of Agricultural and Food Chemistry 60, no. 9 (February 24, 2012): 2123–28. http://dx.doi.org/10.1021/jf202773y.

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5

Benfaremo, Nicholas, and Michael P. Cava. "Studies in anthracycline synthesis: simple Diels-Alder routes to pachybasin, .omega.-hydroxypachybasin, aloe-emodin, and fallacinol." Journal of Organic Chemistry 50, no. 1 (January 1985): 139–41. http://dx.doi.org/10.1021/jo00201a033.

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6

WULANSARI, DEWI, YULIASRI JAMAL, PRAPTIWI, and ANDRIA AGUSTA. "Pachybasin, a Major Metabolite from Culture Broth of Endophytic Coelomyceteous AFKR-18 Fungus isolated from a Yellow Moonsheed Plant, Arcangelisia flava (L.) Merr." HAYATI Journal of Biosciences 21, no. 2 (June 2014): 95–100. http://dx.doi.org/10.4308/hjb.21.2.95.

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7

Hildebrandt, Ulrich, Alexander Marsell, and Markus Riederer. "Direct Effects of Physcion, Chrysophanol, Emodin, and Pachybasin on Germination and Appressorium Formation of the Barley (Hordeum vulgare L.) Powdery Mildew Fungus Blumeria graminis f. sp. hordei (DC.) Speer." Journal of Agricultural and Food Chemistry 66, no. 13 (March 20, 2018): 3393–401. http://dx.doi.org/10.1021/acs.jafc.7b05977.

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8

Bissett, John. "A revision of the genus Trichoderma. III. Section Pachybasium." Canadian Journal of Botany 69, no. 11 (November 1, 1991): 2373–417. http://dx.doi.org/10.1139/b91-298.

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Twenty species assigned to Trichoderma section Pachybasium (Sacc.) Bissett are described and differentiated on the basis of conidiophore and conidium morphology. Included in section Pachybasium are T. hamatum, T. polysporum, T. piluliferum, T. harzianum, and T. virens that were already recognized in Trichoderma; T. flavofuscum comb.nov. transferred from Gliocladium; the anamorphs of Hypocrea gelatinosa, H. semiorbis, and two unnamed Hypocrea species; and 10 new species segregated from the T. hamatum species aggregate of M. A. Rifai (1969. Mycol. Pap. 116: 1–56). The new species Trichoderma crassum, T. croceum, T. fasciculatum, T. fertile, T. longipilis, T. minutisporum, T. oblongisporum, T. pubescens, T. spirale, T. strictipilis, T. strigosum, and T. tomentosum are proposed. Keys are provided to distinguish the 20 known species in section Pachybasium. Key words: Trichoderma section Pachybasium, Hypocrea, taxonomy.
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9

Bissett, John. "A revision of the genus Trichoderma. II. Infrageneric classification." Canadian Journal of Botany 69, no. 11 (November 1, 1991): 2357–72. http://dx.doi.org/10.1139/b91-297.

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The genus Trichoderma Pers.:Fr. is defined to include anamorphs of Hypocrea, previously placed in Gliocladium and Verticillium, having elongate phialides and irregularly branched conidiophores. A sectional classification is proposed for Trichoderma recognizing the following five sections: section Trichoderma, section Longibrachiatum Bissett, section Saturnisporum Doi et al., section Pachybasium (Sacc.) stat.nov., and section Hypocreanum sect.nov. Trichoderma lactea sp.nov. is described, typifying section Hypocreanum. The species in section Trichoderma have narrow and flexuous conidiophores and branches, with branches and phialides uncrowded, frequently paired, and seldom with more than three elements in a whorl. Section Pachybasium includes species with highly ramified conidiophores that are often aggregated into compact fascicles or pustules and with relatively short, broad branches bearing inflated phialides in crowded verticils. Section Hypocreanum accommodates Trichoderma anamorphs of Hypocrea that have effuse conidiation, sparingly branched conidiophores, and cylindrical to subulate phialides. The placement of existing Trichoderma species, species aggregates, and some anamorphs of Hypocrea in the five sections is discussed, and a key is provided to differentiate the sections of Trichoderma. Key words: Trichoderma, sectional classification, Hypocrea, Gliocladium.
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10

Caron, Brigitte, and Paul Brassard. "An integrated approach to the synthesis of contiguously substituted xanthopurpurins, pachybasins and purpurins." Tetrahedron 49, no. 4 (January 1993): 771–84. http://dx.doi.org/10.1016/s0040-4020(01)80322-7.

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11

QIN, WEN-TAO, and WEN-YING ZHUANG. "Seven new species of Trichoderma (Hypocreales) in the Harzianum and Strictipile clades." Phytotaxa 305, no. 3 (April 28, 2017): 121. http://dx.doi.org/10.11646/phytotaxa.305.3.1.

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Among 240 specimens of Trichoderma producing sexual states collected in different regions of China, 80 specimens representing 21 species possess green ascospores. Species were identified based on integrated studies of phenotypic features, culture characteristics and molecular analyses of RNA polymerase II subunit b and translation elongation factor 1-α genes. Of these 21 species, seven are recognized here as new: T. angustum, T. crystalligenum, T. globoides, T. perviride, T. purpureum, T. tenue and T. viridulum. Trichoderma angustum and T. crystalligenum belong to the Strictipile clade, and the remaining five species are in the Harzianum clade. Trichoderma angustum inhabits herbaceous stems and possesses trichoderma- to verticillium-like conidiophores. Trichoderma crystalligenum forms brownish yellow pulvinate stromata, pachybasium-like conidiophores, and abundant yellow crystals in culture. Trichoderma globoides and T. perviride share a common ancestor with but are different from T. alni, T. christiani, T. corneum and T. italicum. Trichoderma purpureum is a sister of T. epimyces, and is distinguished by its purplish red stromata. Trichoderma tenue is closely related to T. priciliae and T. rufobrunneum, and is characterized by verticillium-like conidiophores and slender phialides. Trichoderma viridulum is associated with T. cerinum, T. tomentosum and T. ceraceum; it is distinguishable by trichoderma-like rather than pachybasium-like conidiophores. Morphological distinctions between the new species and their allies are discussed. This study updates our knowledge of species diversity of the group.
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12

Lee, Ching-Fu, and Tzong-Hsiung Hseu. "Genetic relatedness of Trichoderma sect. Pachybasium species based on molecular approaches." Canadian Journal of Microbiology 48, no. 9 (September 1, 2002): 831–40. http://dx.doi.org/10.1139/w02-081.

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Molecular approaches including internal transcribed spacer (ITS) sequences of ribosomal DNA, universal primer polymerase chain reaction (UP-PCR) fingerprinting, and DNA–DNA hybridization were used to study the genetic relatedness of species within Trichoderma sect. Pachybasium. In the analysis of ITS and 5.8S sequences of ribosomal DNA, parsimony analysis demonstrated that forty-one strains were distributed into five main groups supported by high bootstrap values. The species of Trichoderma sect. Pachybasium were clustered into groups I, II, and IV, with the strains of Trichoderma fasculatum and Trichoderma strictipile forming a separate branch, an independent group V. Some species within each group showed nearly identical sequence differences (fewer than 1–3 bp). UP-PCR and DNA–DNA hybridization were further used to clarify the genetic relatedness of these species with highly similar ITS sequences. Highly similar or identical UP-PCR profiles and high values of DNA complementarity (>70%) were observed among some species, Trichoderma hamatum and Trichoderma pubescens; Trichoderma croceum,Trichoderma polysporum and Trichoderma album; Trichoderma crassum and Trichoderma flavofuscum; and Trichoderma strictipile and Trichoderma fasciculatum. Although every species can be differentiated morphologically, the species showed highly similar molecular characteristics in the above cases, indicating that they could be conspecific. However, in some cases (Trichoderma longipile, T. crassum and T. flavofuscum; Trichoderma fertile and Trichoderma minutisporum; Trichoderma tomentosum, Trichoderma inhamatum and Trichoderma harzianum) there were discriminative patterns of UP-PCR and (or) low levels (<50%) of DNA–DNA hybridization; even their ITS sequences were similar, suggesting a closely phylogenetic relationship.Key words: fingerprinting, ITS sequencing, DNA–DNA hybridization, taxonomy, Trichoderma.
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13

CARON, B., and P. BRASSARD. "ChemInform Abstract: An Integrated Approach to the Synthesis of Contiguously Substituted Xanthopurpurins, Pachybasins, and Purpurins." ChemInform 24, no. 19 (August 20, 2010): no. http://dx.doi.org/10.1002/chin.199319224.

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14

Lu, Bingsheng, Irina S. Druzhinina, Payam Fallah, Priscila Chaverri, Cornelia Gradinger, Christian P. Kubicek, and Gary J. Samuels. "Hypocrea/Trichodermaspecies with pachybasium-like conidiophores: teleomorphs forT. minutisporumandT. polysporumand their newly discovered relatives." Mycologia 96, no. 2 (March 2004): 310–42. http://dx.doi.org/10.1080/15572536.2005.11832980.

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15

SITRIN, ROBERT D., GEORGE CHAN, JOHN DINGERDISSEN, CHARLES DEBROSSE, RAJ MEHTA, GERALD ROBERTS, SUE ROTTSCHAEFER, et al. "Isolation and structure determination of Pachybasium cerebrosides which potentiate the antifungal activity of aculeacin." Journal of Antibiotics 41, no. 4 (1988): 469–80. http://dx.doi.org/10.7164/antibiotics.41.469.

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16

Bissett, John, George Szakacs, Carol Ann Nolan, Irina Druzhinina, Cornelia Gradinger, and Christian P. Kubicek. "New species of Trichoderma from Asia." Canadian Journal of Botany 81, no. 6 (June 1, 2003): 570–86. http://dx.doi.org/10.1139/b03-051.

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Seven new species of Trichoderma, isolated from soil or tree bark from Siberia, Nepal, northern India, Taiwan, Thailand, Cambodia, and Malaysia, are described based on morphological and physiological characters, and from their phylogenetic position inferred from parsimony analyses of nucleotide sequences of the internal transcribed spacer (ITS) regions of the rDNA cluster (ITS1 and 2) and partial sequences of translation elongation factor 1-alpha (tef1α). Trichoderma sinensis sp. nov. and Trichoderma effusum sp. nov. are additions to Trichoderma section Longibrachiatum. Trichoderma helicum sp. nov., Trichoderma rossicum sp. nov., Trichoderma velutinum sp. nov., and Trichoderma cerinum sp. nov. are additions to section Pachybasium. Trichoderma erinaceum sp. nov. is described in section Trichoderma. The contribution of sequence data in resolving species in Trichoderma is demonstrated by Trichoderma helicum, which is morphologically indistinguishable from Trichoderma tomentosum Bissett, even though the two species are not phylogenetically closely related.Key words: Hypocrea, systematics, taxonomy, phylogeny.
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17

Nováková, Alena, Alena Kubátová, Šárka Valinová, Vít Hubka, and Miroslav Kolařík. "Trichoderma fassatiae, a new species from the section Pachybasium isolated from soil in the Czech Republic." Czech Mycology 67, no. 2 (October 30, 2015): 227–31. http://dx.doi.org/10.33585/cmy.67205.

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18

Li, Jishun, Yuanzheng Wu, Kai Chen, Yilian Wang, Jindong Hu, Yanli Wei, and Hetong Yang. "Trichoderma cyanodichotomus sp. nov., a new soil-inhabiting species with a potential for biological control." Canadian Journal of Microbiology 64, no. 12 (December 2018): 1020–29. http://dx.doi.org/10.1139/cjm-2018-0224.

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During a biodiversity survey of Trichoderma (Ascomycota, Hypocreales, Hypocreaceae) in coastal and lake wetlands of China, a new species, Trichoderma cyanodichotomus, was isolated from Dongting Lake wetland of Hunan province. The strain TW21990-1 was characterized as having two types of conidia and producing a distinct blue–green pigment on potato dextrose agar and cornmeal dextrose agar. The taxonomic position was analyzed using three molecular markers, internal transcribed spacer rDNA, translation elongation factor 1-alpha, and RNA polymerase II subunit B, revealing less than 95.0% homology with all known Trichoderma species. The combined phylogenetic tree further identified T. cyanodichotomus as an independent subgroup belonging to Section Pachybasium, with no close relatives. In vitro antagonistic activity by dual-culture assay exhibited broad inhibition against various plant pathogens, including Botryosphaeria dothidea, Pythium aphanidermatum, Rhizoctonia solani, and Verticillium dahliae. In addition, TW21990-1 demonstrated moderate hydrolase activity of cellulase, chitinase, β-1,3-glucanase, and protease, which might be involved in mycoparasitism. Greenhouse experiments showed strong biocontrol effects against tomato damping-off incited by P. aphanidermatum, together with increased seedling height and weight gain. The identification of T. cyanodichotomus will provide useful information for sufficient utilization of fungal resources.
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19

Lu, Bingsheng, Irina S. Druzhinina, Payam Fallah, Priscila Chaverri, Cornelia Gradinger, Christian P. Kubicek, and Gary J. Samuels. "Hypocrea/Trichoderma Species with Pachybasium-like Conidiophores: Teleomorphs for T. minutisporum and T. polysporum and Their Newly Discovered Relatives." Mycologia 96, no. 2 (March 2004): 310. http://dx.doi.org/10.2307/3762066.

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20

Dreyfuss, M. M., and W. Gams. "(1140) Proposal to reject Pachybasium niveum Rostr. in order to retain the name Tolypocladium inflatum W. Gams for the fungus that produces cyclosporin." TAXON 43, no. 4 (November 1994): 660–61. http://dx.doi.org/10.2307/1223557.

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21

Komoń-Zelazowska, Monika, John Bissett, Doustmorad Zafari, Lóránt Hatvani, László Manczinger, Sheri Woo, Matteo Lorito, László Kredics, Christian P. Kubicek, and Irina S. Druzhinina. "Genetically Closely Related but Phenotypically Divergent Trichoderma Species Cause Green Mold Disease in Oyster Mushroom Farms Worldwide." Applied and Environmental Microbiology 73, no. 22 (September 7, 2007): 7415–26. http://dx.doi.org/10.1128/aem.01059-07.

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ABSTRACT The worldwide commercial production of the oyster mushroom Pleurotus ostreatus is currently threatened by massive attacks of green mold disease. Using an integrated approach to species recognition comprising analyses of morphological and physiological characters and application of the genealogical concordance of multiple phylogenetic markers (internal transcribed spacer 1 [ITS1] and ITS2 sequences; partial sequences of tef1 and chi18-5), we determined that the causal agents of this disease were two genetically closely related, but phenotypically strongly different, species of Trichoderma, which have been recently described as Trichoderma pleurotum and Trichoderma pleuroticola. They belong to the Harzianum clade of Hypocrea/Trichoderma which also includes Trichoderma aggressivum, the causative agent of green mold disease of Agaricus. Both species have been found on cultivated Pleurotus and its substratum in Europe, Iran, and South Korea, but T. pleuroticola has also been isolated from soil and wood in Canada, the United States, Europe, Iran, and New Zealand. T. pleuroticola displays pachybasium-like morphological characteristics typical of its neighbors in the Harzianum clade, whereas T. pleurotum is characterized by a gliocladium-like conidiophore morphology which is uncharacteristic of the Harzianum clade. Phenotype MicroArrays revealed the generally impaired growth of T. pleurotum on numerous carbon sources readily assimilated by T. pleuroticola and T. aggressivum. In contrast, the Phenotype MicroArray profile of T. pleuroticola is very similar to that of T. aggressivum, which is suggestive of a close genetic relationship. In vitro confrontation reactions with Agaricus bisporus revealed that the antagonistic potential of the two new species against this mushroom is perhaps equal to T. aggressivum. The P. ostreatus confrontation assays showed that T. pleuroticola has the highest affinity to overgrow mushroom mycelium among the green mold species. We conclude that the evolutionary pathway of T. pleuroticola could be in parallel to other saprotrophic and mycoparasitic species from the Harzianum clade and that this species poses the highest infection risk for mushroom farms, whereas T. pleurotum could be specialized for an ecological niche connected to components of Pleurotus substrata in cultivation. A DNA BarCode for identification of these species based on ITS1 and ITS2 sequences has been provided and integrated in the main database for Hypocrea/Trichoderma (www.ISTH.info ).
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22

BENFAREMO, N., and M. P. CAVA. "ChemInform Abstract: STUDIES IN ANTHRACYCLINE SYNTHESIS: SIMPLE DIELS-ALDER ROUTES TO PACHYBASIN, Ω-HYDROXYPACHYBASIN, ALOE-EMODIN, AND FALLACINOL." Chemischer Informationsdienst 16, no. 22 (June 4, 1985). http://dx.doi.org/10.1002/chin.198522167.

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