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Journal articles on the topic "Oxygen uptake rates"
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Mines, Richard O. "Oxygen transfer parameters and oxygen uptake rates revisited." Journal of Environmental Science and Health, Part A 55, no. 4 (November 28, 2019): 345–53. http://dx.doi.org/10.1080/10934529.2019.1694817.
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Rotem, Avi, Menmet Toner, Ronald G. Tompkins, and Martin L. Yarmush. "Oxygen uptake rates in cultured rat hepatocytes." Biotechnology and Bioengineering 40, no. 10 (December 5, 1992): 1286–91. http://dx.doi.org/10.1002/bit.260401020.
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Kizaki, Z., and R. G. Thurman. "Stimulation of oxygen uptake by glucagon is oxygen dependent in perfused rat liver." American Journal of Physiology-Gastrointestinal and Liver Physiology 256, no. 2 (February 1, 1989): G369—G376. http://dx.doi.org/10.1152/ajpgi.1989.256.2.g369.
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Livers from well-fed female Sprague-Dawley rats (100-150 g) were perfused at flow rates of 4 or 8 ml.g liver-1.min-1 to deliver O2 to the organ at various rates. During perfusion at normal flow rates (4 ml.g-1.min-1), glucagon (10 nM) increased O2 uptake in perfused liver by approximately 40 mumol.g-1.h-1. In contrast, glucagon increased O2 uptake by nearly 100 mumol.g-1.h-1 when livers were perfused at high flow rates. Increase in O2 uptake was directly proportional to flow rate and was blocked partially by infusion of phorbol myristate acetate (100 nM) before glucagon. Increase in O2 uptake due to elevated flow was not due to enhanced glucagon delivery, since infusion of 120 nM glucagon at normal flow rates only increased O2 uptake by approximately 40 mumol.g-1.h-1. On the other hand, when O2 tension in the perfusate was manipulated at normal flow rates, the stimulation of O2 uptake by glucagon increased proportional to the average O2 tension in the liver. Infusion of 8-bromo-adenosine 3',5'-cyclic monophosphate (BrcAMP; 25 microM) also increased O2 uptake more than twice as much at high compared with normal flow rates. In the presence of angiotensin II (5 nM), a hormone that increases intracellular calcium, glucagon increased O2 uptake by nearly 100 mumol.g-1.h-1 at normal flow rates. Infusion of glucagon or BrcAMP into livers perfused at normal flow rates increased state 3 rates of O2 uptake of subsequently isolated mitochondria significantly by approximately 25%. In contrast, perfusion with glucagon or BrcAMP at high flow rates increased mitochondrial respiration by 50-60%. Glucagon addition acutely to suspensions of mitochondria, however, had no effect on O2 uptake. These data are consistent with reports that glucagon administration in vivo or treatment of intact cells with glucagon increases O2 uptake of subsequently isolated mitochondria, a phenomenon that can account for the observed increase in O2 uptake in livers perfused at high flow rates with glucagon. Furthermore, these results are consistent with the hypothesis that the effect of glucagon on mitochondria is O2 dependent in the perfused liver. This is most likely due to an effect of intracellular calcium on a mechanism mediated via cAMP.(ABSTRACT TRUNCATED AT 250 WORDS)
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Qu, Wei, Zhi Zhong, Gavin E. Arteel, and Ronald G. Thurman. "Stimulation of oxygen uptake by prostaglandin E2 is oxygen dependent in perfused rat liver." American Journal of Physiology-Gastrointestinal and Liver Physiology 275, no. 3 (September 1, 1998): G542—G549. http://dx.doi.org/10.1152/ajpgi.1998.275.3.g542.
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The aim of this study was to determine if the effect of prostaglandin E2(PGE2) on hepatic oxygen uptake was affected by oxygen tension. Livers from fed female Sprague-Dawley rats were perfused at normal or high flow rates (4 or 8 ml ⋅ g−1 ⋅ min−1) to vary local oxygen tension within the liver lobule. During perfusion at normal flow rates, PGE2 (5 μM) infusion increased oxygen uptake by about 50 μmol ⋅ g−1 ⋅ h−1; however, when livers were perfused at high flow rates, the increase was nearly twice as large. Simultaneously, glucose output was increased rapidly by about 50%, whereas glycolysis was decreased about 60%. When flow rate was held constant, increases in oxygen uptake due to PGE2 were proportional to oxygen delivery. Infusion of PGE2 into livers perfused at normal flow rates increased state 3 rates of oxygen uptake of subsequently isolated mitochondria by about 25%; however, rates were increased 50–75% in mitochondria isolated from livers perfused at high flow rates. Thus it is concluded that PGE2stimulates oxygen uptake via mechanisms regulated by oxygen tension in perfused rat liver. High flow rates also increased basal rates of oxygen uptake: this increase was prevented by inactivation of Kupffer cells with GdCl3. In addition, conditioned medium from Kupffer cells incubated at high oxygen tension (75% oxygen) stimulated oxygen uptake of isolated parenchymal cells by >30% and elevated PGE2production about twofold compared with Kupffer cells exposed to normal air-saturated buffer (21% oxygen). These effects were blocked completely by both indomethacin and nisoldipine. These data support the hypothesis that oxygen stimulates Kupffer cells to release mediators such as PGE2 which elevate oxygen consumption in parenchymal cells, possibly by mechanisms involving cyclooxygenase and calcium channels.
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Carlson, John S., and Geraldine A. Naughton. "An Examination of the Anaerobic Capacity of Children Using Maximal Accumulated Oxygen Deficit." Pediatric Exercise Science 5, no. 1 (February 1993): 60–71. http://dx.doi.org/10.1123/pes.5.1.60.
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The purpose of this study was to determine the anaerobic capacity of children using the maximal accumulated oxygen deficit technique (AOD). Eighteen healthy children (9 boys, 9 girls) with a mean age of 10.6 years volunteered as subjects. Peak oxygen uptake and submaximal steady-state oxygen uptake tests were conducted against progressive constant work rates on a Cybex cycle ergometer. Supramaximal work rates were predicted from the linear regression of submaximal steady-state work rates and oxygen uptakes to equal 110, 130, and 150% of peak oxygen uptake. Results indicated a significant interaction in the responses of both sexes when the accumulated oxygen deficit data were expressed in both absolute and relative terms. The profile of accumulated oxygen deficits across the three intensities indicated a downward shift in the girls responses between the 110 and 150% supramaximal tests. This trend was not evident in the boys’ responses. Intraclass correlations conducted on test-retest data indicated that compared to the boys, the reliability of the girls in the accumulated oxygen deficits in liters and ml·kg−1 was poorer.
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Nakagawa, Y., T. Matsumura, M. Goto, W. Qu, F. C. Kauffman, and R. G. Thurman. "Increase in oxygen uptake due to arachidonic acid is oxygen dependent in the perfused liver." American Journal of Physiology-Gastrointestinal and Liver Physiology 266, no. 5 (May 1, 1994): G953—G959. http://dx.doi.org/10.1152/ajpgi.1994.266.5.g953.
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The purpose of this study was to determine whether the effect of arachidonic acid on hepatic O2 uptake is O2 dependent and which region of the liver lobule it affects. In livers perfused at normal flow rates, infusion of arachidonate increased O2 uptake significantly by about 20-25 mumol.g-1.h-1. When the flow rate was doubled to make the hepatic O2 gradient shallower, the increase in O2 uptake due to arachidonate was two to three times larger (i.e., approximately 50 mumol.g-1.h-1). In livers perfused in the retrograde direction, maximal rates of O2 uptake were about twofold higher in upstream pericentral than in downstream periportal regions, and arachidonic acid nearly doubled O2 uptake in downstream areas without affecting rates in upstream regions. Thus it is concluded that arachidonate stimulates O2 uptake in an O2-dependent manner. This effect was sensitive to an inhibitor of the lipoxygenase, nordihydroguaiaretic acid, in perfused liver but not in isolated hepatocytes. In addition, conditioned medium from Kupffer cells incubated at high O2 tension stimulated parenchymal cell O2 uptake. Furthermore, arachidonate increased intracellular Ca2+ in isolated Kupffer cells in a dose-dependent manner. These findings suggest that eicosanoids produced by nonparenchymal cells participate in a hepatic O2 sensor mechanism involving Ca2+ that regulates O2 uptake by parenchymal cells in the liver.
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Garcı́a-Ochoa, F., E. Gómez Castro, and V. E. Santos. "Oxygen transfer and uptake rates during xanthan gum production." Enzyme and Microbial Technology 27, no. 9 (November 2000): 680–90. http://dx.doi.org/10.1016/s0141-0229(00)00272-6.
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Huang, Jerry Y. C., Meng-Dawn Cheng, and James T. Mueller. "Oxygen uptake rates for determining microbial activity and application." Water Research 19, no. 3 (1985): 373–81. http://dx.doi.org/10.1016/0043-1354(85)90098-3.
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Peddie, F. L., W. J. Simpson, B. V. Kara, Sarah C. Robertson, and J. R. M. Hammond. "MEASUREMENT OF ENDOGENOUS OXYGEN UPTAKE RATES OF BREWERS' YEASTS." Journal of the Institute of Brewing 97, no. 1 (January 2, 1991): 21–25. http://dx.doi.org/10.1002/j.2050-0416.1991.tb01048.x.
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Milley, J. R. "Uptake of exogenous substrates during hypoxia in fetal lambs." American Journal of Physiology-Endocrinology and Metabolism 254, no. 5 (May 1, 1988): E572—E578. http://dx.doi.org/10.1152/ajpendo.1988.254.5.e572.
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Fetal uptakes of oxygen, glucose, lactate, and alpha-amino-nitrogen containing substrates were measured in eight near-term fetal lambs by simultaneously drawing blood samples from the umbilical vein and descending aorta, then measuring umbilical blood flow using the microsphere method. These procedures were repeated after 3 h of hypoxia induced by lowering maternal inspired oxygen concentration. On the next day the experiment was repeated, except the ewes were first made hypoxic then allowed to breathe room air. These conditions decreased the delivery of oxygen, but not the delivery of other metabolic substrates to the fetus. During hypoxia, fetal oxygen uptake was 82% of normal (mean of both days); fetal glucose and amino-nitrogen uptakes were 74 and 23% of normal, respectively, and fetal lactate uptake became insignificant. These data indicate that endogenous rather than exogenous substrates are used to support fetal oxidative metabolism during hypoxia. Also, because exogenous uptake of amino-nitrogen is less than normal nitrogen accretion rates, fetal growth must be reduced as a consequence of 3-4 h of hypoxia.
Dissertations / Theses on the topic "Oxygen uptake rates"
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Petrella, Robert J. "Left ventricular diastole and oxygen uptake at maximal and submaximal work rates." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/nq21310.pdf.
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Cakici, Avni. "Self-tuning PI control of industrial wastewater treatment process and simultaneous on-line estimations of oxygen uptake and transfer rates." Thesis, University of Exeter, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.317288.
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Wos, Melissa Louise, and n/a. "Methods For Understanding Bacterial Metabolic Activity In Activated Sludge." Griffith University. School of Environmental Engineering, 2005. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20061031.151641.
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Biological wastewater treatment relies on the diverse and complex metabolic activities of bacteria to remove pollutants. Its success depends on the metabolic efficiency of the bacteria. Activated sludge models use parameters that attempt to depict bacterial growth and metabolic processes. However, current methods do not separate metabolic activity from growth and maintenance. As a result, activated sludge processes are misinterpreted or over-simplified. Alternative methods for gauging bacterial activity have been proposed and include the measurements of cellular derived compounds that relate specifically to energy cycling and include Nicotinamide Adenine Dinucleotide [NADH]. To date, NADH has been largely measured within activated sludge using commercial online fluorimeters with in situ probes. However, this current method provides a measure of the 'bulk' (raw) fluorescence within the system, resulting in difficulties when interpreting fluorescence data and poor sensitivity for detecting changes in intracellular [NADH]. This study has developed a more reliable method for estimating intracellular [NADH] and thus metabolic activity within activated sludge systems. Separating extracellular from intracellular [NADH] in samples was crucial because NADH was released and accumulates in the extracellular environment at a concentration of 200 ~M immediately following bacterial death or lysis. This concentration did not decline overtime. This not only caused high background fluorescence but also reduced the sensitivity of detection for changes in intracellular [NADH]. In particular, considerably higher [NADH] values to those from the extracellular suspensions were obtained following extraction of the intracellular material, suggesting that the cell membranes were not being penetrated by the excitable light source. Of the extraction procedures examined, filtration followed by extraction of the intracellular material with a hot Tris buffer was the most efficient and was recommended for accurate estimates of intracellular [NADH] in situ. In addition, standards were used to quantify NADH (moles per cell and/or unit volume) from unknown samples. The limits of detection were found to be 1.058 - 353 uM, whereas concentrations above 353 jAM self-quenched. Sample concentrations were always within these limits of detection. Hence, the sensitivity, reliability and experimental application of the original method was improved upon and able to be used for the direct measurement of microbial metabolic activity, something that has not been demonstrated before now. This study found that bacteria have between 106~ I 08 NADH molecules per cell depending on their metabolic state. A highly metabolically active bacterial cell had between 1O6~ tO7 NADH molecules, while a less active bacterial cell had between to7 -to8 NADH molecules. These measurements of metabolic activity were simultaneously monitored alongside other measures of bacterial growth, such as the incorporation of radiolabelled thymidine into DNA as a direct measure of DNA replication (new cell synthesis), the incorporation of radiolabelled leucine into protein as a direct measure of protein synthesis, oxygen uptake rates (OUR) as a direct measure of respiration, ATP as a measure of potential energy and dissolved organic carbon (DOC) as a measure of substrate assimilation. As OUR deceased, bacterial growth (using both the thymidine and leucine assays), specific [NADH] and specific [ATP] increased. High OUR and substrate oxidation rates simultaneous with low specific [NADH] indicated high rates of electron transport and thus efficient metabolic activity. Also, low OUR and substrate oxidation rates simultaneous with high specific [NADHI indicated inefficient rates of electron transport, therefore inhibiting oxidative phosphorylation (ATP production). A lack of oxygen as the terminal electron acceptor did not efficiently reoxidise NADH to NAD and resulted in an accumulation of NADH within the cell. Thus, a measure of low specific [NADHI was linked to the efficient rate of reoxidation of NADH to NAD* and reflects high metabolic efficiency. DNA and protein syntheses were coupled following substrate enrichment (glucose or acetate), indicating that bacteria were in balanced growth. However, DNA and protein syntheses became uncoupled once substrate was depleted, indicating unbalanced growth. An average Leu:TdR ratio of 7.4 was determined for activated sludge and was comparable to values published from marine systems. This ratio increased during log growth phase and decreased during stationary growth phases. Specific growth rates determined using the [3HITdR and [3H]Leu assay yielded values ranging from 2 - 10.5 d' and from 2.5 - 6 d1, respectively and were comparable to published values. Changes in OUR, NADH, ATE', DNA replication and protein synthesis were statistically ordinated using multidimensional scaling, and changes (in magnitude and direction) in bacterial metabolic activity were observed. Such methods enable the tracing of where bacteria divert their energies, such as to growth or maintenance and thus provide a greater understanding of bacterial behaviour in activated sludge. While studying anoxic and anaerobic conditions were beyond the scope of this work, the use of such methods to monitor bacterial metabolic activity under such conditions is warranted.
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Wos, Melissa Louise. "Methods For Understanding Bacterial Metabolic Activity In Activated Sludge." Thesis, Griffith University, 2005. http://hdl.handle.net/10072/367303.
Full textAbstract:
Biological wastewater treatment relies on the diverse and complex metabolic activities of bacteria to remove pollutants. Its success depends on the metabolic efficiency of the bacteria. Activated sludge models use parameters that attempt to depict bacterial growth and metabolic processes. However, current methods do not separate metabolic activity from growth and maintenance. As a result, activated sludge processes are misinterpreted or over-simplified. Alternative methods for gauging bacterial activity have been proposed and include the measurements of cellular derived compounds that relate specifically to energy cycling and include Nicotinamide Adenine Dinucleotide [NADH]. To date, NADH has been largely measured within activated sludge using commercial online fluorimeters with in situ probes. However, this current method provides a measure of the 'bulk' (raw) fluorescence within the system, resulting in difficulties when interpreting fluorescence data and poor sensitivity for detecting changes in intracellular [NADH]. This study has developed a more reliable method for estimating intracellular [NADH] and thus metabolic activity within activated sludge systems. Separating extracellular from intracellular [NADH] in samples was crucial because NADH was released and accumulates in the extracellular environment at a concentration of 200 ~M immediately following bacterial death or lysis. This concentration did not decline overtime. This not only caused high background fluorescence but also reduced the sensitivity of detection for changes in intracellular [NADH]. In particular, considerably higher [NADH] values to those from the extracellular suspensions were obtained following extraction of the intracellular material, suggesting that the cell membranes were not being penetrated by the excitable light source. Of the extraction procedures examined, filtration followed by extraction of the intracellular material with a hot Tris buffer was the most efficient and was recommended for accurate estimates of intracellular [NADH] in situ. In addition, standards were used to quantify NADH (moles per cell and/or unit volume) from unknown samples. The limits of detection were found to be 1.058 - 353 uM, whereas concentrations above 353 jAM self-quenched. Sample concentrations were always within these limits of detection. Hence, the sensitivity, reliability and experimental application of the original method was improved upon and able to be used for the direct measurement of microbial metabolic activity, something that has not been demonstrated before now. This study found that bacteria have between 106~ I 08 NADH molecules per cell depending on their metabolic state. A highly metabolically active bacterial cell had between 1O6~ tO7 NADH molecules, while a less active bacterial cell had between to7 -to8 NADH molecules. These measurements of metabolic activity were simultaneously monitored alongside other measures of bacterial growth, such as the incorporation of radiolabelled thymidine into DNA as a direct measure of DNA replication (new cell synthesis), the incorporation of radiolabelled leucine into protein as a direct measure of protein synthesis, oxygen uptake rates (OUR) as a direct measure of respiration, ATP as a measure of potential energy and dissolved organic carbon (DOC) as a measure of substrate assimilation. As OUR deceased, bacterial growth (using both the thymidine and leucine assays), specific [NADH] and specific [ATP] increased. High OUR and substrate oxidation rates simultaneous with low specific [NADH] indicated high rates of electron transport and thus efficient metabolic activity. Also, low OUR and substrate oxidation rates simultaneous with high specific [NADHI indicated inefficient rates of electron transport, therefore inhibiting oxidative phosphorylation (ATP production). A lack of oxygen as the terminal electron acceptor did not efficiently reoxidise NADH to NAD and resulted in an accumulation of NADH within the cell. Thus, a measure of low specific [NADHI was linked to the efficient rate of reoxidation of NADH to NAD* and reflects high metabolic efficiency. DNA and protein syntheses were coupled following substrate enrichment (glucose or acetate), indicating that bacteria were in balanced growth. However, DNA and protein syntheses became uncoupled once substrate was depleted, indicating unbalanced growth. An average Leu:TdR ratio of 7.4 was determined for activated sludge and was comparable to values published from marine systems. This ratio increased during log growth phase and decreased during stationary growth phases. Specific growth rates determined using the [3HITdR and [3H]Leu assay yielded values ranging from 2 - 10.5 d' and from 2.5 - 6 d1, respectively and were comparable to published values. Changes in OUR, NADH, ATE', DNA replication and protein synthesis were statistically ordinated using multidimensional scaling, and changes (in magnitude and direction) in bacterial metabolic activity were observed. Such methods enable the tracing of where bacteria divert their energies, such as to growth or maintenance and thus provide a greater understanding of bacterial behaviour in activated sludge. While studying anoxic and anaerobic conditions were beyond the scope of this work, the use of such methods to monitor bacterial metabolic activity under such conditions is warranted.Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Environmental Engineering
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Brink-Elfegoun, Thibault. "Limitations of maximal oxygen uptake during whole-body exercise /." Stockholm : Karolinska institutet, 2007. http://diss.kib.ki.se/2007/20071116brin/.
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Jarvis, David R. "Multifrequent work rate forcings in the assessment of oxygen uptake kinetics." Thesis, Sheffield Hallam University, 1999. http://shura.shu.ac.uk/19870/.
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During dynamic exercise, the response of the cardiorespiratory system is structured to maintain homeostasis at the cellular level. The rate at which homeostasis is established is largely dependent on the system's structural and physiological integrity. Evidence suggests that any impairment in the functioning of the system might be reflected in a determination of oxygen uptake (VO[2]) kinetics. The kinetics of VO[2] have been quantified in response to step, impulse, ramp and sinusoidal changes in work rate (WR). An alternative approach uses a technique in which the WR is perturbed according to a pseudorandom binary signal. Pseudorandom binary sequence (PRBS) WR forcings have the advantage of being able to provide a determination of vo2 kinetics from a single test session of ~30 min duration. The assessment of VO[2]kinetics using PRBS WR forcings demands that the controlling process behaves in a linear manner. To minimise the contribution of non-linear influences, changes in work intensity must be constrained to the sub-lactate threshold domain. When examining clinical, untrained or young subjects, the necessary reduction in the upper work limit of a PRBS forcing can effect a fall in the distribution of power across the bandwidth of the sequence. If the distribution of power should fall below a critical level, then it can become difficult to elicit discernible responses from the forcing. To resolve this problem, this thesis investigated the potential for developing a multifrequent WR forcing altered to enhance identification of the underlying VO[2] response. The multifrequent WR forcing developed for use in this thesis took the form of a binary sequence. Binary transitions were determined according to a specially constructed multifrequent signal. Signal construction involved redistributing the available signal power to specific harmonics in a chosen range of frequencies. To validate estimates of VO[2] kinetics derived from the multifrequent binary sequence (MFBS) WR forcing, comparisons were made with the data obtained from an established PRBS forcing. When comparing physiological data, it is necessary to consider the amount of variability between trials. Therefore, prior to assessing the agreement between data obtained from the MFBS and PRBS methods, this thesis sought to establish the degree of variability in estimates of VO[2] kinetics derived from PRBS exercise tests. The results presented in this thesis show estimates of the mean response time (MRT) of VO[2] derived from the MFBS method to be 46.8 (4.2) s (mean (standard deviation) seconds), compared with 45.2 (5.0) s for the PRBS method. This suggests that the two methods yield comparable determinations of VO[2] kinetics. Supporting evidence is provided by the limits of agreement. These indicate that the maximum difference likely to occur between the MRT obtained from the two methods (-6.5 to +9.6 s) is less than that expected due to variability in the MRT derived from PRBS forcings (-11.6 to +8.0 s). However, the limits also reveal the poor repeatability of VO[2] response data obtained from the PRBS used in the thesis. Consequently, the use of this data to assess the validity of t the MRT derived from MFBS forcings is not recommended. In addition to poor repeatability, the possibility exists that assessments of VO[2] kinetics derived from MFBS WR forcings will also depend on the distribution of power across the harmonic content of the sequence. Therefore, whilst MFBS WR forcings may be suited to the assessment of VO[2] kinetics in subjects with a reduced tolerance to exercise, there remain doubts concerning both the validity of the response data and applicability of the method. Until these issues have been resolved, care would need to be taken when using estimates of VO[2] kinetics derived from MFBS WR forcings to determine the functional state of the cardiorespiratory system.
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Chandra, Sanjay. "Evaluation of oxygen uptake rate as an activated sludge process control parameter." Thesis, Virginia Polytechnic Institute and State University, 1987. http://hdl.handle.net/10919/101173.
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A debate currently exists concerning whether or not oxygen uptake rate is a valid control parameter for monitoring the activated sludge process. A laboratory study was conducted to attempt to shed light on the controversy. Two bench-scale reactors were operated at steady state and under shock load. Oxygen uptake rate (OUR) was measured with the BOD bottle technique and with an on-line respirometer. The reliability of the results obtained from the BOD bottle technique was also of interest.
No relationship could be deduced between effluent quality and oxygen uptake rate thereby suggesting that the latter would not be useful as a control parameter. As was concluded from the shock load data, the oxygen uptake rate varies very inconsistently at high organic loadings.
It was found that the BOD bottle technique completely failed at very high organic loadings and gave meaningless results. The on-line respirometer, in spite of its high sensitivity, gave more realistic and consistent results.M.S.
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Blackwood, Susan Kim. "Aspects of fitness and physical activity patterns in Edinburgh school children." Thesis, Open University, 1997. http://oro.open.ac.uk/57657/.
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There is growing concern that many children in Britain do not take sufficient exercise to benefit cardiovascular health. This is supported by extensive evidence advocating the importance of regular physical activity for lifelong health and well-being, and is of particular relevance in Scotland given it's notorious record of adult coronary heart disease death. This study examined aspects of fitness and physical activity in groups of Edinburgh school children, aged between 13 and 14 years. A three stage investigation was adopted: Phase One: A repeated measures, same subject design was used to examine the reliability and validity of selected measures (20m shuttle run test, peak oxygen uptake (V02 Peak), and anthropometric measures). Thirty three children (15 boys, 18 girls) performed each test on 3 separate days. Anthropometric measures showed strong reliability (r > 0.94, n=33) whilst reliability for the treadmill test ofV02 peak and shuttle run performance was lower (r=0.89 and r=0.79 respectively). Multiple regression analysis yielded a new equation for predicting V02 peak for children. This age specific prediction equation incorporated shuttle run performance in conjunction with skinfold thickness measures (Boys, R2=0.64 SEE=3.46; Girls, R2=0.79, SEE=2.81). Repeat testing was also recommended. Phase Two: An evaluation of methods of heart rate data analysis to assess physical activity in children. Twenty eight children (14 boys, 14 girls) wore continuous heart rate monitors (polar Electro PE4000, Finland) over a period of 7 days (Monday to Sunday), mean duration 737 (+/-55) mins/day. A detailed 7 day self report activity diary was also completed. Variability of heart rate measures was high (R=0.10-0.30), and it was noted that using data for just 4 days or less resulted in considerable underestimation of total weekly activity levels (44-100% error). If activity levels are to be compared against current recommendations, researchers must endeavour to achieve weekly rather than daily estimates of activity. Evaluation of methods of heart rate data analysis showed good correlation between heart rate activity indices and reported seven day activity. For boys strongest correlation was achieved using the number of 5 minute periods with HR > 139 b.min"l and the number of 5 minute periods with HR > 50% heart rate reserve (r=0.80, n=14). Total activity time was similar for both males and females but girls engaged in fewer sustained bouts of activity (>5 minutes) and a better correlate with activity in females was achieved using the total number of elevated heart rates (total HR > 50% heart rate reserve, r=0.64, n=14). Phase Three: A cross sectional survey was conducted to investigate standards of aerobic fitness and patterns of physical activity in groups of Edinburgh school children. Height, weight, skinfold thickness, shuttle run performance and physical activity (assessed by heart rate monitoring and activity diary) were recorded in a sample of 91 children (44 Boys, 47 Girls). Overall, males performed significantly better on the shuttle run test (t=5.4, df=88, p < 0.05), had higher predicted peak oxygen uptake (t=5.6, df=87, p < 0.05), and engaged in more bouts of moderate to vigorous activity than females. Seventy percent of boys and 50% of girls fulfilled current physical activity guidelines. Most activities were school based (131 mins per week as compared to 85 mins per week of out of school activities). Activity tended to be sporadic with active days interspersed with inactive days (mean 3.2 +/·1.6 days per week). After school activities specifically targeting young girls should be promoted.
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Sirna, Karen Margaret. "The effect of body position on oxygen uptake and heart rate kinetics in older people." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0007/MQ32509.pdf.
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Wójcik, Weronika. "Evaluation of microbiological activity during the deammonification process for nitrogen removal." Thesis, KTH, VA-teknik, Vatten, Avlopp och Avfall, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-96300.
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This master thesis is based on own studies. A four-month study was performed at Hammarby Sjostad Research Station, which is located in Stockholm. One-stage deammonification process was evaluated in two different system configurations in pilot plant scale. The theoretical background for this thesis works is presented in the first part and where is presented negative impacts of nitrogen compounds in environment and requirements for purified wastewater in European Union (Sweden and Poland). In the next part of the thesis the nitrogen cycle is described and with focus on biological reactions for nitrogen removal. Especially, nitrification/denitrification and anammox processes are described with special focus on parameters affecting the anammox process performance and its advantages and disadvantages of using this process. Experimental results from the four-month study and evaluation of the microbial activity are described in the last part.
Books on the topic "Oxygen uptake rates"
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Mahon, Anthony D. Aerobic training. Oxford University Press, 2013. http://dx.doi.org/10.1093/med/9780199232482.003.0039.
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Aerobic training is defined as exercise training that involves whole body endurance activity that is sustained for a sufficient length of time and at a sufficient intensity in order to improve cardiorespiratory fitness.1 The effect of aerobic training on physiological function in children has been investigated for nearly four decades. Some of this research has focused on the health-related benefits of this type of training on children and adolescents and for good reason. With increasing rates of obesity, type 2 diabetes, metabolic syndrome, and many other physical inactivity-related disorders, there is ample reason to discern the health-related effects of aerobic training during the paediatric years.2,3 However, there also has been a concerted effort to study the effect of aerobic training on the physiological adaptations, particularly maximal oxygen uptake ( V · O 2 max), that are associated with endurance performance.4 This chapter will focus on the latter consideration and will examine the effect of aerobic training in apparently healthy children and adolescents.
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Kinnear, William, and James H. Hull. A Practical Guide to the Interpretation of Cardiopulmonary Exercise Tests. 2nd ed. Oxford University Press, 2021. http://dx.doi.org/10.1093/med/9780198834397.001.0001.
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A Practical Guide to the Interpretation of Cardiopulmonary Exercise Tests is a short, but comprehensive, guide for those who are involved in the supervision of exercise tests and interpretation of cardiopulmonary exercise test (CPET) data. It is a clear and concise guide which will also be of interest to those who request CPETs and who wish to understand more about how to use the results. The first four chapters cover the reasons why a CPET may be requested, pre-test assessment, supervision of the test to ensure that it is safe, and the practicalities of the test itself. Subsequent chapters look in detail at the key CPET measurements of heart rate, ventilation, oxygen uptake, and carbon dioxide output. There are chapters on the parameters that can be derived from these basic measurements: ventilatory equivalents, oxygen pulse, and the respiratory exchange ratio. Further chapters show how the anaerobic threshold and respiratory compensation point are obtained, and how they can be used to interpret the physiological response to exercise. The role of monitoring oxygen saturation and flow–volume loops during the test is described. The third section of the book has chapters on presentation of results, interpretative strategies, and summaries of classical CPET patterns seen in different diseases. The final chapters consider the role of preoperative CPET testing and how to prescribe exercise. All chapters have pointers to further resources.
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Wilton, Niall, Brian J. Anderson, and Bruno Marciniak. Anatomy, physiology, and pharmacology in paediatric anaesthesia. Edited by Jonathan G. Hardman and Neil S. Morton. Oxford University Press, 2017. http://dx.doi.org/10.1093/med/9780199642045.003.0069.
Full textAbstract:
Anaesthesia for children is tempered by changes that occur during both growth and development. Drug dose is affected by size and clearance maturation processes as well as the changing body composition that occurs with age. All organ systems undergo these maturation changes and most are complete within the first few years of life. Normal physiological variables in infancy and childhood are quite different from adults. The central nervous, cardiovascular, and respiratory systems are particularly important. Cerebral immaturity and plasticity impacts sensitivity to drugs, pain responses, and behaviour and increases potential harm from apoptosis with anaesthesia. The heart undergoes a transition from fetal to adult circulation during the first few weeks of life. Undiagnosed congenital defects are not uncommon. The neonate is very susceptible to conditions that trigger an increase in pulmonary vascular resistance, with reversion to fetal circulatory patterns. Respiratory anatomy and mechanics affect the propensity to apnoea, airway maintenance, artificial ventilation modalities, uptake of inhalational agents, and tracheal tube sizes. Metabolic rate and oxygen requirements increase with decreasing age. This physiology influences diverse aspects that include the rate of desaturation during apnoea, hypoglycaemia during starvation, cardiac output, drug metabolism, fluid requirements, and heat production or loss.
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Armstrong, Neil, and Joanne R. Welsman. Aerobic fitness. Oxford University Press, 2013. http://dx.doi.org/10.1093/med/9780199232482.003.0008.
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Maximal oxygen uptake ( V O 2 · max), the highest rate at which an individual can consume O2 during exercise, is widely recognized as the best single measure of adults’ aerobic fitness.1,2 Maximal V O 2 · ultimately limits an individual’s capacity to perform aerobic exercise but it does not describe fully all aspects of aerobic fitness. The transient kinetics of V O 2 · best reflect the integrated response of the O2 delivery system and the metabolic requirements of the exercising muscle to rapid changes in exercise intensity. Furthermore, V O 2 · max is not the best index of an individual’s ability to sustain submaximal aerobic exercise and despite its derivation from anaerobic metabolism, measures of blood lactate accumulation during submaximal exercise provide useful indicators of aerobic fitness.As the assessment of V O 2 · kinetics is addressed in Chapter 22, in this chapter, we will focus on the assessment of V O 2 · max and blood lactate accumulation.
Book chapters on the topic "Oxygen uptake rates"
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Bock, A., Y. Genzel, and U. Reichl. "Monitoring of Cell Activity: Online Oxygen Uptake Rates in Pulsed Aerated Cell Culture." In Cells and Culture, 391–93. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_68.
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Nielsen, L. K., T. K. K. Wong, J. Power, S. Reid, and P. F. Greenfield. "Using Oxygen Uptake Rates to Time Infections in The Baculovirus Expression Vector System." In Animal Cell Technology: Basic & Applied Aspects, 161–66. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0848-5_23.
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Gimenes, Maria Antonieta P., Luiz Cláudio S. Carlos, Luís F. F. Faria, and Nei Pereira. "Oxygen Uptake Rate in Production of Xylitol by Candida guilliermondii with Different Aeration Rates and Initial Xylose Concentrations." In Biotechnology for Fuels and Chemicals, 1049–59. Totowa, NJ: Humana Press, 2002. http://dx.doi.org/10.1007/978-1-4612-0119-9_85.
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Haas, J., T. Tröbs, C. Clemens, M. Wunder, M. Rüger, and T. W. Schulz. "Online Determination of Oxygen Uptake and Carbon Dioxide Production Rates in Mammalian Cell Culture Using Mass Spectrometry." In Cells and Culture, 683–87. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_118.
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Binkhorst, R. A., P. F. J. D. Verstraten, I. A. Beekman, and M. A. van’t Hof. "Armergometry in Determining Oxygen Uptake for Work form Heart Rate." In Advances in Ergometry, 468–70. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76442-4_67.
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Cruz, C., K. Joeris, C. Goudar, C. Zhang, and K. Konstantinov. "Oxygen Uptake Rate (OUR) Estimation in High Density Mammalian Cell Perfusion Cultures." In Cells and Culture, 837–41. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_147.
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Preissmann, Anja, Rolf G. Werner, and Wolfgang Noé. "Monitoring and control of large — scale vaccine production via dynamic oxygen uptake rate measurement." In Animal Cell Technology, 169–74. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5404-8_28.
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Kathy Wong, T. K., Lars K. Nielsen, Paul F. Greenfield, and Steven Reid. "Relationship between oxygen uptake rate and time of infection of Sf9 insect cells infected with a recombinant baculovirus." In Cell Culture Engineering IV, 157–67. Dordrecht: Springer Netherlands, 1994. http://dx.doi.org/10.1007/978-94-011-0257-5_18.
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Ismail, Ahmad Rasdan, Norfadzilah Jusoh, Nor Kamilah Makhtar, Raemy Md Zein, Muhamad Mat Noor, and Khairul Azhar Mat Daud. "Optimization of Temperature and Relative Humidity with Maximum Oxygen Uptake (VO2max) and Heart Rate (HR) by Using Response Surface Methodology (RSM)." In Human-Centered Technology for a Better Tomorrow, 723–34. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-16-4115-2_58.
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Wessel, H. U., M. H. Paul, and R. L. Stout. "Oxygen Uptake ( % MathType!MTEF!2!1!+- % feaagaart1ev2aaatCvAUfeBSjuyZL2yd9gzLbvyNv2CaerbuLwBLn % hiov2DGi1BTfMBaeXatLxBI9gBaerbd9wDYLwzYbItLDharqqtubsr % 4rNCHbGeaGqiVu0Je9sqqrpepC0xbbL8F4rqqrFfpeea0xe9Lq-Jc9 % vqaqpepm0xbba9pwe9Q8fs0-yqaqpepae9pg0FirpepeKkFr0xfr-x % fr-xb9adbaqaaeGaciGaaiaabeqaamaabaabaaGcbaacbaGab8Nvay % aacaWaaSbaaSqaaiaa-9gadaWgaaadbaGaa8NmaaqabaaaleqaaOGa % aiikaiaa-rhacaGGPaGaeyypa0Jab8NvayaacaWaaSbaaSqaaiaa-9 % gadaWgaaadbaGaa8NmaaqabaaaleqaaOGaaiikaiaa-nhacaWFZbGa % aiykamaadmaabaGaaGymaiabgkHiTiaa-vgadaahaaWcbeqaaiaa-1 % cacaWF0bGaa83laGGaaiab+r8a0baaaOGaay5waiaaw2faaaaa!4B40! $$ \dot V_{o_2 } (t) = \dot V_{o_2 } (ss)\left[ {1 - e^{ - t/\tau } } \right] $$ ) Response to Progressive Work Rate Increments of One and Four Minute Duration in Pediatric Patients with Congenital Cardiac Defects." In Pediatric Cardiology, 246–48. New York, NY: Springer New York, 1986. http://dx.doi.org/10.1007/978-1-4613-8598-1_65.
Full textConference papers on the topic "Oxygen uptake rates"
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Starly, Binil, and Shih Feng Lan. "Real time measurement of cellular Oxygen Uptake Rates (OUR) by a fiber optic sensor." In 2009 IEEE International Conference on Virtual Environments, Human-Computer Interfaces and Measurements Systems (VECIMS). IEEE, 2009. http://dx.doi.org/10.1109/vecims.2009.5068878.
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Li, Lingling, Bo Yang, and Maojuan Bai. "Impact of High Salinity on Specific Oxygen Uptake, Nitrification and Denitrification Rates of Activated Sludge." In 2009 3rd International Conference on Bioinformatics and Biomedical Engineering (iCBBE). IEEE, 2009. http://dx.doi.org/10.1109/icbbe.2009.5162926.
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Eluvathingal, Sebastian J., Narcisse A. N’Dri, Mark Stremler, and David Cliffel. "Computational Modeling of a Cell-Based Microphysiometer." In ASME 2006 2nd Joint U.S.-European Fluids Engineering Summer Meeting Collocated With the 14th International Conference on Nuclear Engineering. ASMEDC, 2006. http://dx.doi.org/10.1115/fedsm2006-98496.
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A computational model of a cell-based microphysiometer is presented in this paper. The microphysiometer is a fluid based device that uses electrochemical sensors to measure the concentration of metabolites in the fluid medium around living cells. A computational code has been used to model the convective-diffusive transport in this system. This work focuses on modeling an oxygen electrochemical sensor. An ideal sensor model is used to study the effects of initial concentration and cell uptake rate on the sensor signal. In particular, the relative influence of the oxygen consumption by the sensor and the cells is examined. Removing the effect of the sensor allows isolation of cell behavior for various cell uptake rates and ranges of initial concentration. A preliminary comparison of computational results with experimental data is presented. The computational model provides very useful predictions of trends.
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Kim, Jinsoo, Ciaran O’Neill, Kavya Pathak, Sai Shanthanand Rajagopal, Martina Moyne, Glen Picard, J. Andrew Taylor, and Conor Walsh. "Automatically Triggered FES Rowing Device for SCI Patients With Motorized Return." In 2018 Design of Medical Devices Conference. American Society of Mechanical Engineers, 2018. http://dx.doi.org/10.1115/dmd2018-6824.
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A quarter of a million people in the United States are affected by spinal cord injury (SCI), which causes loss of sensation and muscle function. Improvements in clinical care have resulted in a lower risk of mortality from initial complications like bedsores or urosepsis, though patients are more susceptible to long term conditions like coronary heart disease [1], which is a leading cause of death for SCI patients [2]. Patients with SCI have sedentary lifestyles, decreased aerobic fitness, and limited levels of oxygen uptake, which contribute to increased rates of cardiovascular complications [2]. To mitigate these factors, SCI patients must perform vigorous aerobic exercise, which can be done through functional electrical stimulation (FES) [3].
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Rojas, Allan A., Chengdong Yuan, Dmitrii A. Emelianov, Emil R. Saifullin, Seyedsaeed Mehrabi-Kalajahi, Mikhail A. Varfolomeev, Vladislav A. Sudakov, et al. "A 3-Step Reaction Model For Numerical Simulation of In-Situ Combustion." In SPE Russian Petroleum Technology Conference. SPE, 2021. http://dx.doi.org/10.2118/206430-ms.
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Abstract In-situ combustion (ISC) is an effective thermal enhanced oil recovery method. However, it is still not widely implemented in oilfields. One of the factors limiting the wide application of ISC is the challenge in its simulation and prediction. In this work, the oxidation experiments of maltenes and asphaltenes in reservoir rock were performed in the porous media thermo-effect cell (PMTEC) to establish a simplified reaction model based on non-isothermal measurements and to use it in numerical simulation of ISC process. It was found that the oxidation reaction process of oil fractions can be divided into different regions depending on generated self-energy rate and oxygen consumption rates that is up to the temperature. In order to propagate reactions from one mode to another, a specific oxygen consumption per unit mass of oil fractions is required. The average oxygen requirement for crossing LTOad (low temperature oxidation, oxygen addition reactions) boundary into LTC (low temperature combustion) mode was 64 mgO2/g(maltenes) and 10.4 mgO2/g(asphaltenes). To propagate reactions into HTO mode from the LTC mode, it requires about 646 mgO2/g(asphaltenes) for asphaltenes fraction. Moreover, this characterization seems to be a key tool when designing air injection in field pilots. Additionally, it was revealed that asphaltenes are more exothermic and require lower oxygen uptake per unit of temperature increment in comparison to maltenes. Furthermore, the mass conversion data obtained from non-isothermal measurements of oil fractions allow for the estimation of the stoichiometry coefficients of two low temperature oxidation reactions, i.e. oxidation and cocking processes, which can be included into a numerical simulation model to replicate combustion tube (CT) results. The numerical simulation model reveals that the simplified reaction model from a 6-step into a 3-step reaction scheme can reproduce ignition process, temperature profiles, combustion velocity, and fluid production, which thus makes it suitable for the upscaled modelling of ISC.
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Karlsen, Vibeke B., Gamunu Samarakoon, and Carlos Dinamarca. "A Comparative Model-Analysis on Sulphide Bio-oxidation with Different Electron Acceptors." In 63rd International Conference of Scandinavian Simulation Society, SIMS 2022, Trondheim, Norway, September 20-21, 2022. Linköping University Electronic Press, 2022. http://dx.doi.org/10.3384/ecp192014.
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Sulphide (H2S, HS- and S2-) is an undesired by-product of biogas production processes. This modelling work in Aquasim was carried out to study three parallel processes related to sulphide in AD processes: 1) H2S liquid-gas mass transfer; 2) Acid-base equilibrium; and 3) Sulphide oxidation with three different electron acceptors; nitrate, oxygen, and a biotic anode with a given potential. Multiplicative Monod (biotic processes) and Nernst-Monod kinetics (bioelectrochemical process) provide the basis for the sulphide bio-oxidation processes. At the current stage, the model can be used to study sulphide bio-oxidation and the effect of relevant parameters, including initial biomass concentration, uptake rates, temperature, and pH. The model can be improved further by implementing anaerobic microbial processes as competing reactions. With the proposed improvements, the model can be a useful tool for calculating the chemical dosage or electrode potential required for sulphide removal. These calculations can be based on both the concentration of H2S(g) in the headspace (ppm) often available at full-scale plants and the concentration of sulphide (HS-(liq)) in effluent streams from the plants.
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Laszczyk, Piotr. "Predictive functional control of dissolved oxygen with online estimation of oxygene uptake rate." In 2015 20th International Conference on Methods and Models in Automation and Robotics (MMAR ). IEEE, 2015. http://dx.doi.org/10.1109/mmar.2015.7283943.
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Pedev, Todor, Ljubomir Mladenov, Anton Gochkov, and Silvia Shandurkova. "OXYGEN SATURATION AND HEART RATE VARIABILITY DURING ORIENTEERING RACES." In INTERNATIONAL SCIENTIFIC CONGRESS “APPLIED SPORTS SCIENCES”. Scientific Publishing House NSA Press, 2022. http://dx.doi.org/10.37393/icass2022/80.
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ABSTRACT Orienteering is a hard, physically demanding sport. The combination of running and map reading tests competitors’ mental and physical endurance. The high speed during races requires an intense oxygen uptake. This paper discusses oxygen deficiency during orienteering races. Competitors’ oxygen saturation and heart rate values were monitored by smart watches and pulse oximeters. A group of eight male elite orienteers were tested from March 2021 to October 2021 through laboratory and terrain tests. The laboratory spiroergometry was carried out on a treadmill. Spiroergometric parameters and gas analysis results were recorded in real time (breath-by-breath) with a Meta Max 3B metabolic analyzer. The terrain tests - pulmometry and measurement of oxygen levels in the blood (SpО2) were taken by Huawei GT Pro 2 watches. Indicators of movement, speed per kilometer, average and maximum heart rate values were collected. Field studies to monitor oxygen levels in the blood (SpO2) were conducted during orienteering competitions for the first time in Bulgaria. Descriptive statistics, hypothesis testing, and correlation analysis were used for data analysis. The research results revealed that the orienteers do not suffer oxygen deficiency during orienteering races, which led us to the conclusion that the level of oxygen in the blood (SpО2) does not influence technical errors during the competitions. Statistically significant differences in the average heart rate values of the subjects for forest versus sprint disciplines were observed, which implies specialization of the competitors by discipline. Further studies should be done for interpreting and comparing the terrain tests data with the laboratory functional tests by taking into account the individual characteristics of each competitor.
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"Effects of Sediment Resuspension and Oxygenation on Oxygen Uptake Rate." In 2014 ASABE Annual International Meeting. American Society of Agricultural and Biological Engineers, 2014. http://dx.doi.org/10.13031/aim.20141896896.
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Premanoch, Piyarat. "Short-term biochemical oxygen demand (BODst) estimation using an oxygen uptake rate measurement method." In 2016 Management and Innovation Technology International Conference (MITicon). IEEE, 2016. http://dx.doi.org/10.1109/miticon.2016.8025257.
Full textReports on the topic "Oxygen uptake rates"
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Solberg, Thomas. Aspects of anuran metabolism : effects of chronic hypoxia on maximal oxygen uptake rates and the fate of lactic acid. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.3215.
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Shenker, Moshe, Paul R. Bloom, Abraham Shaviv, Adina Paytan, Barbara J. Cade-Menun, Yona Chen, and Jorge Tarchitzky. Fate of Phosphorus Originated from Treated Wastewater and Biosolids in Soils: Speciation, Transport, and Accumulation. United States Department of Agriculture, June 2011. http://dx.doi.org/10.32747/2011.7697103.bard.
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Beneficial use of reclaimed wastewater (RW) and biosolids (BS) in soils is accompanied by large input of sewage-originated P. Prolonged application may result in P accumulation up to levelsBeneficial use of reclaimed wastewater (RW) and biosolids (BS) in soils is accompanied by large input of sewage-originated P. Prolonged application may result in P accumulation up to levels that impair plant nutrition, increase P loss, and promote eutrophication in downstream waters. This study aims to shed light on the RW- and BS-P forms in soils and to follow the processes that determine P reactivity, solubility, availability, and loss in RW and BS treated soils. The Technion group used sequential P extraction combined with measuring stable oxygen isotopic composition in phosphate (δ18OP) and with 31P-NMR studies to probe P speciation and transformations in soils irrigated with RW or fresh water (FW). The application of the δ18OP method to probe inorganic P (Pi) speciation and transformations in soils was developed through collaboration between the Technion and the UCSC groups. The method was used to trace Pi in water-, NaHCO3-, NaOH-, and HCl- P fractions in a calcareous clay soil (Acre, Israel) irrigated with RW or FW. The δ18OP signature changes during a month of incubation indicated biogeochemical processes. The water soluble Pi (WSPi) was affected by enzymatic activity yielding isotopic equilibrium with the water molecules in the soil solution. Further it interacted rapidly with the NaHCO3-Pi. The more stable Pi pools also exhibited isotopic alterations in the first two weeks after P application, likely related to microbial activity. Isotopic depletion which could result from organic P (PO) mineralization was followed by enrichment which may result from biologic discrimination in the uptake. Similar transformations were observed in both soils although transformations related to biological activity were more pronounced in the soil treated with RW. Specific P compounds were identified by the Technion group, using solution-state 31P-NMR in wastewater and in soil P extracts from Acre soils irrigated by RW and FW. Few identified PO compounds (e.g., D-glucose-6-phosphate) indicated coupled transformations of P and C in the wastewater. The RW soil retained higher P content, mainly in the labile fractions, but lower labile PO, than the FW soil; this and the fact that P species in the various soil extracts of the RW soil appear independent of P species in the RW are attributed to enhanced biological activity and P recycling in the RW soil. Consistent with that, both soils retained very similar P species in the soil pools. The HUJ group tested P stabilization to maximize the environmental safe application rates and the agronomic beneficial use of BS. Sequential P extraction indicated that the most reactive BS-P forms: WSP, membrane-P, and NaHCO3-P, were effectively stabilized by ferrous sulfate (FeSul), calcium oxide (CaO), or aluminum sulfate (alum). After applying the stabilized BS, or fresh BS (FBS), FBS compost (BSC), or P fertilizer (KH2PO4) to an alluvial soil, P availability was probed during 100 days of incubation. A plant-based bioassay indicated that P availability followed the order KH2PO4 >> alum-BS > BSC ≥ FBS > CaO-BS >> FeSul-BS. The WSPi concentration in soil increased following FBS or BSC application, and P mineralization further increased it during incubation. In contrast, the chemically stabilized BS reduced WSPi concentrations relative to the untreated soil. It was concluded that the chemically stabilized BS effectively controlled WSPi in the soil while still supplying P to support plant growth. Using the sequential extraction procedure the persistence of P availability in BS treated soils was shown to be of a long-term nature. 15 years after the last BS application to MN soils that were annually amended for 20 years by heavy rates of BS, about 25% of the added BS-P was found in the labile fractions. The UMN group further probed soil-P speciation in these soils by bulk and micro X-ray absorption near edge structure (XANES). This newly developed method was shown to be a powerful tool for P speciation in soils. In a control soil (no BS added), 54% of the total P was PO and it was mostly identified as phytic acid; 15% was identified as brushite and 26% as strengite. A corn crop BS amended soil included mostly P-Fe-peat complex, variscite and Al-P-peat complex but no Ca-P while in a BS-grass soil octacalcium phosphate was identified and o-phosphorylethanolamine or phytic acid was shown to dominate the PO fraction that impair plant nutrition, increase P loss, and promote eutrophication in downstream waters. This study aims to shed light on the RW- and BS-P forms in soils and to follow the processes that determine P reactivity, solubility, availability, and loss in RW and BS treated soils. The Technion group used sequential P extraction combined with measuring stable oxygen isotopic composition in phosphate (δ18OP) and with 31P-NMR studies to probe P speciation and transformations in soils irrigated with RW or fresh water (FW). The application of the δ18OP method to probe inorganic P (Pi) speciation and transformations in soils was developed through collaboration between the Technion and the UCSC groups. The method was used to trace Pi in water-, NaHCO3-, NaOH-, and HCl- P fractions in a calcareous clay soil (Acre, Israel) irrigated with RW or FW. The δ18OP signature changes during a month of incubation indicated biogeochemical processes. The water soluble Pi (WSPi) was affected by enzymatic activity yielding isotopic equilibrium with the water molecules in the soil solution. Further it interacted rapidly with the NaHCO3-Pi. The more stable Pi pools also exhibited isotopic alterations in the first two weeks after P application, likely related to microbial activity. Isotopic depletion which could result from organic P (PO) mineralization was followed by enrichment which may result from biologic discrimination in the uptake. Similar transformations were observed in both soils although transformations related to biological activity were more pronounced in the soil treated with RW. Specific P compounds were identified by the Technion group, using solution-state 31P-NMR in wastewater and in soil P extracts from Acre soils irrigated by RW and FW. Few identified PO compounds (e.g., D-glucose-6-phosphate) indicated coupled transformations of P and C in the wastewater. The RW soil retained higher P content, mainly in the labile fractions, but lower labile PO, than the FW soil; this and the fact that P species in the various soil extracts of the RW soil appear independent of P species in the RW are attributed to enhanced biological activity and P recycling in the RW soil. Consistent with that, both soils retained very similar P species in the soil pools. The HUJ group tested P stabilization to maximize the environmental safe application rates and the agronomic beneficial use of BS. Sequential P extraction indicated that the most reactive BS-P forms: WSP, membrane-P, and NaHCO3-P, were effectively stabilized by ferrous sulfate (FeSul), calcium oxide (CaO), or aluminum sulfate (alum). After applying the stabilized BS, or fresh BS (FBS), FBS compost (BSC), or P fertilizer (KH2PO4) to an alluvial soil, P availability was probed during 100 days of incubation. A plant-based bioassay indicated that P availability followed the order KH2PO4 >> alum-BS > BSC ≥ FBS > CaO-BS >> FeSul-BS. The WSPi concentration in soil increased following FBS or BSC application, and P mineralization further increased it during incubation. In contrast, the chemically stabilized BS reduced WSPi concentrations relative to the untreated soil. It was concluded that the chemically stabilized BS effectively controlled WSPi in the soil while still supplying P to support plant growth. Using the sequential extraction procedure the persistence of P availability in BS treated soils was shown to be of a long-term nature. 15 years after the last BS application to MN soils that were annually amended for 20 years by heavy rates of BS, about 25% of the added BS-P was found in the labile fractions. The UMN group further probed soil-P speciation in these soils by bulk and micro X-ray absorption near edge structure (XANES). This newly developed method was shown to be a powerful tool for P speciation in soils. In a control soil (no BS added), 54% of the total P was PO and it was mostly identified as phytic acid; 15% was identified as brushite and 26% as strengite. A corn crop BS amended soil included mostly P-Fe-peat complex, variscite and Al-P-peat complex but no Ca-P while in a BS-grass soil octacalcium phosphate was identified and o-phosphorylethanolamine or phytic acid was shown to dominate the PO fraction.
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Brosh, Arieh, David Robertshaw, Yoav Aharoni, Zvi Holzer, Mario Gutman, and Amichai Arieli. Estimation of Energy Expenditure of Free Living and Growing Domesticated Ruminants by Heart Rate Measurement. United States Department of Agriculture, April 2002. http://dx.doi.org/10.32747/2002.7580685.bard.
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Research objectives were: 1) To study the effect of diet energy density, level of exercise, thermal conditions and reproductive state on cardiovascular function as it relates to oxygen (O2) mobilization. 2) To validate the use of heart rate (HR) to predict energy expenditure (EE) of ruminants, by measuring and calculating the energy balance components at different productive and reproductive states. 3) To validate the use of HR to identify changes in the metabolizable energy (ME) and ME intake (MEI) of grazing ruminants. Background: The development of an effective method for the measurement of EE is essential for understanding the management of both grazing and confined feedlot animals. The use of HR as a method of estimating EE in free-ranging large ruminants has been limited by the availability of suitable field monitoring equipment and by the absence of empirical understanding of the relationship between cardiac function and metabolic rate. Recent developments in microelectronics provide a good opportunity to use small HR devices to monitor free-range animals. The estimation of O2 uptake (VO2) of animals from their HR has to be based upon a consistent relationship between HR and VO2. The question as to whether, or to what extent, feeding level, environmental conditions and reproductive state affect such a relationship is still unanswered. Studies on the basic physiology of O2 mobilization (in USA) and field and feedlot-based investigations (in Israel) covered a , variety of conditions in order to investigate the possibilities of using HR to estimate EE. In USA the physiological studies conducted using animals with implanted flow probes, show that: I) although stroke volume decreases during intense exercise, VO2 per one heart beat per kgBW0.75 (O2 Pulse, O2P) actually increases and measurement of EE by HR and constant O2P may underestimate VO2unless the slope of the regression relating to heart rate and VO2 is also determined, 2) alterations in VO2 associated with the level of feeding and the effects of feeding itself have no effect on O2P, 3) both pregnancy and lactation may increase blood volume, especially lactation; but they have no effect on O2P, 4) ambient temperature in the range of 15 to 25°C in the resting animal has no effect on O2P, and 5) severe heat stress, induced by exercise, elevates body temperature to a sufficient extent that 14% of cardiac output may be required to dissipate the heat generated by exercise rather than for O2 transport. However, this is an unusual situation and its affect on EE estimation in a freely grazing animal, especially when heart rate is monitored over several days, is minor. In Israel three experiments were carried out in the hot summer to define changes in O2P attributable to changes in the time of day or In the heat load. The animals used were lambs and young calves in the growing phase and highly yielding dairy cows. In the growing animals the time of day, or the heat load, affected HR and VO2, but had no effect on O2P. On the other hand, the O2P measured in lactating cows was affected by the heat load; this is similar to the finding in the USA study of sheep. Energy balance trials were conducted to compare MEI recovery by the retained energy (RE) and by EE as measured by HR and O2P. The trial hypothesis was that if HR reliably estimated EE, the MEI proportion to (EE+RE) would not be significantly different from 1.0. Beef cows along a year of their reproductive cycle and growing lambs were used. The MEI recoveries of both trials were not significantly different from 1.0, 1.062+0.026 and 0.957+0.024 respectively. The cows' reproductive state did not affect the O2P, which is similar to the finding in the USA study. Pasture ME content and animal variables such as HR, VO2, O2P and EE of cows on grazing and in confinement were measured throughout three years under twenty-nine combinations of herbage quality and cows' reproductive state. In twelve grazing states, individual faecal output (FO) was measured and MEI was calculated. Regression analyses of the EE and RE dependent on MEI were highly significant (P<0.001). The predicted values of EE at zero intake (78 kcal/kgBW0.75), were similar to those estimated by NRC (1984). The EE at maintenance condition of the grazing cows (EE=MEI, 125 kcal/kgBW0.75) which are in the range of 96.1 to 125.5 as presented by NRC (1996 pp 6-7) for beef cows. Average daily HR and EE were significantly increased by lactation, P<0.001 and P<0.02 respectively. Grazing ME significantly increased HR and EE, P<0.001 and P<0.00l respectively. In contradiction to the finding in confined ewes and cows, the O2P of the grazing cows was significantly affected by the combined treatments (P<0.00l ); this effect was significantly related to the diet ME (P<0.00l ) and consequently to the MEI (P<0.03). Grazing significantly increased O2P compared to confinement. So, when EE of grazing animals during a certain season of the year is estimated using the HR method, the O2P must be re measured whenever grazing ME changes. A high correlation (R2>0.96) of group average EE and of HR dependency on MEI was also found in confined cows, which were fed six different diets and in growing lambs on three diets. In conclusion, the studies conducted in USA and in Israel investigated in depth the physiological mechanisms of cardiovascular and O2 mobilization, and went on to investigate a wide variety of ruminant species, ages, reproductive states, diets ME, time of intake and time of day, and compared these variables under grazing and confinement conditions. From these combined studies we can conclude that EE can be determined from HR measurements during several days, multiplied by O2P measured over a short period of time (10-15 min). The study showed that RE could be determined during the growing phase without slaughtering. In the near future the development microelectronic devices will enable wide use of the HR method to determine EE and energy balance. It will open new scopes of physiological and agricultural research with minimizes strain on animals. The method also has a high potential as a tool for herd management.