Log in

Relevant bibliographies by topics / Oxidising enzymes

Contents

Journal articles
Dissertations / Theses
Book chapters

Academic literature on the topic 'Oxidising enzymes'

Author: Grafiati

Published: 4 June 2021

Last updated: 9 February 2022

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Oxidising enzymes.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Oxidising enzymes"

1

Gorton, Lo. "Special issue on sugar oxidising enzymes." Bioelectrochemistry 135 (October 2020): 107577. http://dx.doi.org/10.1016/j.bioelechem.2020.107577.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Urbanska, Anna, W. Freddy Tjallingii, Anthony F. G. Dixon, and Bogumil Leszczynski. "Phenol oxidising enzymes in the grain aphid's saliva." Entomologia Experimentalis et Applicata 86, no. 2 (February 1998): 197–203. http://dx.doi.org/10.1046/j.1570-7458.1998.00281.x.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

Wang, Ting, Liang Liang Wang, and Xun Li. "Cloning, Expression and Characterization of a Monooxygenase P450BM3 from Bacillus megaterium ALA2." Advanced Materials Research 518-523 (May 2012): 5533–38. http://dx.doi.org/10.4028/www.scientific.net/amr.518-523.5533.

Full text

Abstract:

Cytochrome P450 monooxygenases are enzymes which are capable of oxidising saturated and unsaturated substrates. P450BM3 from Bacillus megaterium is one of this family. For the ﬁrst time, the cyp gene for coding P450BM3 from B. megaterium ALA2 has been cloned and expressed in Escherichia coli. The recombinant enzyme is 120 kDa, containing 1049 aa. The highest activity of purified enzyme is 14.8 U/mg towards palmitic acid by monitoring the NADPH oxidation. The optimal pH and temperature were 9.0 and 40°C. The enzyme has higher activity towards linoleic acid, and 2-Methyl-7-octadecene can also be catalyzed which is a precursor of displar.

APA, Harvard, Vancouver, ISO, and other styles

4

Wagenführ, André, Sören Tech, and Holger Unbehaun. "Modifizierung der Holzeigenschaften durch Enzyme | Modification of wood properties through enzymes." Schweizerische Zeitschrift fur Forstwesen 156, no. 11 (November 1, 2005): 420–26. http://dx.doi.org/10.3188/szf.2005.0420.

Full text

Abstract:

The addition of phenol oxidising enzyme preparations leads to a change in the fibre structure of lignocellulose products. This means that positive material properties can be selectively put in. In the production of materials the use of activated ownfibre binding forces only makes sense when the production process ensures that fibres are as close to one another as possible. This can be accomplished by fine tuning the density of the material to the method in question. Material densities of over 600 kg/m3 are necessary for dry method approaches. With wet methods, on the other hand, the properties of material can be improved starting from a density of 160 kg/m3. A good distribution and the use of additionally created hydro-bridge builders are responsible for this. The employment of charge carriers during the processing with a wet method also has a positive influence on the physical properties. The suspended particles that become detached from the fibre in the process bind together and are concentrated on the surface of the fibre. To improve the steering of the process with the wet method further enzymatic treatment steps in lignocellulose can be carried out with micro- and nano-particle systems. This can be done via combinations of cationic starch or cationic polyacrylamide. With these systems the cationic polymer is added to the fibre suspension first, followed by the micro-particle components. Both the levels of additives used and the required incubation times can thereby be markedly reduced. Here for the first time we succeeded in using enzymes in processing technically relevant dimensions and were therefore able to renounce the addition of synthetic binding means without impairing the properties of the material.

APA, Harvard, Vancouver, ISO, and other styles

5

Pillay, C. S., and C. Dennison. "Cathepsin B Stability, But Not Activity, Is Affected in Cysteine:Cystine Redox Buffers." Biological Chemistry 383, no. 7-8 (August 27, 2002): 1199–204. http://dx.doi.org/10.1515/bc.2002.132.

Full text

Abstract:

Abstract In order to test the hypothesis that the lysosomal cysteine protease cathepsin B may be redox regulated in vivo, cathepsin B activity and stability were measured in cysteine and/or cystinecontaining buffers. Cathepsin B activity in cysteinecontaining buffers was similar at pH 6.0 and pH 7.0, over all thiol concentrations tested. In contrast, the stability of the enzyme was greater at pH 6.0 than at pH 7.0. This suggests that the enzymes operational pH in vivo may be < pH 7.0. The activity of the enzyme was depressed in glutathionecontaining buffers. When assessed in cysteine:cystine redox buffers (pH 6.0 7.0) cathepsin B was active over a broad redox potential range, suggesting that cathepsin B activity may not be redox regulated. However, at pH 7.0, the stability of cathepsin B decreased with increasing reduction potential and ambient cystine concentration. This suggests that the stability of the enzyme at neutral pH is dependent on redox potential, and on the presence of oxidising agents.

APA, Harvard, Vancouver, ISO, and other styles

6

MacAodha, Domhnall, Peter Ó Conghaile, Brenda Egan, Paul Kavanagh, Christoph Sygmund, Roland Ludwig, and Dónal Leech. "Comparison of Glucose Oxidation by Crosslinked Redox Polymer Enzyme Electrodes Containing Carbon Nanotubes and a Range of Glucose Oxidising Enzymes." Electroanalysis 25, no. 1 (December 5, 2012): 94–100. http://dx.doi.org/10.1002/elan.201200536.

Full text

APA, Harvard, Vancouver, ISO, and other styles

7

Iftikhar, Mehwish, Lin Wang, and Zhijie Fang. "Synthesis of 1-Deoxynojirimycin: Exploration of Optimised Conditions for Reductive Amidation and Separation of Epimers." Journal of Chemical Research 41, no. 8 (August 2017): 460–64. http://dx.doi.org/10.3184/174751917x15000341607489.

Full text

Abstract:

1-Deoxynojirimycin (DNJ), which has importance with respect to sugar processing enzymes, is a synthetic target for chemists. A key step in the synthesis of DNJ is the preparation of 2,3,4,6-tetra- O-benzyl-D-glucono-δ-lactam. By varying reaction parameters such as temperature, solvent and reducing reagent, improvements on previous methods are described. A novel approach for the synthesis of 2,3,4,6-tetra- O-benzyl-5-dehydro-5-deoxo-D-gluconamide has been developed by using PCC as an oxidising agent. Separation of epimers permitted DNJ to be obtained in 85% yield after reduction and hydrogenolysis steps.

APA, Harvard, Vancouver, ISO, and other styles

8

Chauhan, P. K., Rishma ., V. Singh, and Abhishek B. "Comparaative study of Lipid profile and level of Antioxidant enzymes in cigarette smokers with non cigaretee smokers." Indian Journal of Pharmaceutical and Biological Research 1, no. 01 (January 31, 2013): 55–62. http://dx.doi.org/10.30750/ijpbr.1.1.5.

Full text

Abstract:

Cigarette smoking is the serious health problems and most important avoidable cause of death in world. Worldwide more than 8 million people currently die each year from smoking half of them before of the age of 60. Every cigarette reduces the life span by about 5 minutes. Smoke contains oxidising agents and the oxidation reactions can produce free radicals. In turn, these radicals can start chain reactions that damage cells. In the present study 40 male subjects were divided into four different groups and their lipid profile have been estimated by various tests i.e. Cholesterol, Triglyceride, HDL-C, LDLC, VLDL-C. It was observed that in cigarette smokers HDL-C level decreased and cholesterol, triglyceride, LDL-C, VLDL-C level increased as compared to the control i.e. non- cigarette smokers. In case of MDA and Antioxidant enzymes test, the value of MDA increases and antioxidant enzymes decreases in cigarette smokers as compared to the control i.e. non- cigarette smokers. The variation in the level of lipid profile and antioxidant enzymes from normal values causes several diseases such as Lung cancer, other cancers, heart disease, and stroke and has numerous immediate health effects on the brain and on the respiratory, cardiovascular, gastrointestinal, immune systems.

APA, Harvard, Vancouver, ISO, and other styles

9

Rajashekar, N., and T. C. Shivashankara Murthy. "Toxicological aspects of pendimethalin induced activities of certain oxidising and hydrolytic enzymes in the germinating seedlings of maize (Zea maysL.)." Archives Of Phytopathology And Plant Protection 43, no. 3 (February 2010): 296–301. http://dx.doi.org/10.1080/03235400701804018.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Tsuchiya, Yugo, Sew Yeu Peak-Chew, Clare Newell, Sheritta Miller-Aidoo, Sriyash Mangal, Alexander Zhyvoloup, Jovana Bakovic´, et al. "Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells." Biochemical Journal 474, no. 14 (July 11, 2017): 2489–508. http://dx.doi.org/10.1042/bcj20170129.

Full text

Abstract:

Coenzyme A (CoA) is an obligatory cofactor in all branches of life. CoA and its derivatives are involved in major metabolic pathways, allosteric interactions and the regulation of gene expression. Abnormal biosynthesis and homeostasis of CoA and its derivatives have been associated with various human pathologies, including cancer, diabetes and neurodegeneration. Using an anti-CoA monoclonal antibody and mass spectrometry, we identified a wide range of cellular proteins which are modified by covalent attachment of CoA to cysteine thiols (CoAlation). We show that protein CoAlation is a reversible post-translational modification that is induced in mammalian cells and tissues by oxidising agents and metabolic stress. Many key cellular enzymes were found to be CoAlated in vitro and in vivo in ways that modified their activities. Our study reveals that protein CoAlation is a widespread post-translational modification which may play an important role in redox regulation under physiological and pathophysiological conditions.

APA, Harvard, Vancouver, ISO, and other styles

More sources

Dissertations / Theses on the topic "Oxidising enzymes"

1

Preneta, A. Z. "Studies on lactate oxidising enzymes and their application to ferrocene-based enzyme electrodes for lactate." Thesis, Cranfield University, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376191.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Hsueh, Li-Ching. "Studies on proline hydroxylases." Thesis, University of Oxford, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.365805.

Full text

APA, Harvard, Vancouver, ISO, and other styles

3

McCully, S. "Characterisation of the principal human pulmonary drug-oxidising enzymes, CYP3A5, CYP2B6 & FMO." Thesis, University of Aberdeen, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.593066.

Full text

Abstract:

The human lung has been shown to possess a wide range of enzyme systems and the potential to metabolise xenobiotics. Despite this, there is a paucity of well-designed investigations on the expression of pulmonary xenobiotic-metabolising enzymes. This thesis aimed to characterise the principal pulmonary drug-oxidising enzymes (CYP3A5, CPY2B6 & FMO) and to assess the role of this organ in the metabolism of therapeutic agents. CYP3A5 and CYP2B6 mRNAs were detected in 75% and 100% of lungs, respectively, and CYP3A5 and CYP2B6 protein was detected in 33% of lung samples, with 11% samples coexpressing these enzymes. Testosterone 6β-hydroxylation (CYP3A4/5) and 16β-hydroxylation (CYP2B6/7) were approximately 1500- and 700-fold lower in lung than liver, respectively. FMO activity was 29-fold lower in lung microsomes compared with liver. The ability of human lung microsomes to metabolise KC11458, terfenadine, cyclophosphamide and ifosfamide was investigated. KC11458 was metabolised to KC13195 in human lung samples by CYP2B6, whereas KC11458 was primarily metabolised to metabolite 1 by CYO3A4/5 and FMO in human liver. No terfenadine metabolites were detected in human lung incubations, whereas terfenadine alcohol formation was detected in all 8 livers. Human liver samples activated both ifosfamide and cyclophosphamide, whereas only ifosfamide activation was detected in human lung microsomes. This is of interest as lung cancer is the biggest cause of cancer mortality worldwide and ifosfamide is important in cancer therapy. CYP3A expression has been demonstrated in lung tumours, suggesting ifosfamide could be activated in situ. This study has illustrated the functional expression of specific drug-oxidising enzymes within the lung and has demonstrated that his organ can biotransform and bioactivate therapeutic agents with a metabolite profile significantly different from that of the liver. This may have clinical significance and may be important in determining the activation of compounds having local toxic or therapeutic actions within the lung (e.g. ifosfamide).

APA, Harvard, Vancouver, ISO, and other styles

4

Alandas, Mohammed N. "An investigation into the metabolic activation of novel chloromethylindolines by isoforms of cytochrome P450. Targeting drug metabolising enzymes in cancer: analysis of the role and function of selected cytochrome P450 oxidising novel cancer prodrugs." Thesis, University of Bradford, 2012. http://hdl.handle.net/10454/6289.

Full text

Abstract:

Introduction Cytochromes P450 (CYPs) are the major family of enzymes responsible for detoxification and metabolism of a wide range of both endogenous and xenobiotics chemicals in living organisms. The use of CYPs to activate prodrugs to cytotoxins selectively in tumours has been explored including AQ4N, Phortress and Aminoflavone. CYP1A1, CYP1B1, CYP2W1, and CYP4F11 have been identified as expressed in tumour tissue and surrounding stroma at high frequency compared to most normal tissues. Aim is to investigate the differential metabolism of novel chloromethylindoline by high frequency expressed CYPs in tumours. This differential may be exploited to elicit a selective chemotherapeutic effect by metabolising inert small molecules to potent cytotoxins within the tumour environment. Materials and Methods Sensitive and specific LC/MS/MS techniques have been developed to investigate the metabolism of chloromethylindolines. Recombinant enzymes and transfected cell lines were used to investigate the metabolic profiles with a focus on production of the cytotoxic derivatives of chloromethylindolines. Results Detailed metabolic studies show that (1-(Chloromethyl)-1,2-dihydropyrrolo [3,2-e]indol-3(6H)-yl)(5-methoxy-1H-indol-2-yl) methanone (ICT2700) and other chloromethylindolines are converted by CYP1A1 mediated hydroxylation at the C-5 position leading to highly potent metabolites. In vitro cytotoxicity studies showed differentials of up to 1000-fold was achieved between CYP1A1 activated compared to the non-metabolised parent molecules. The reactivity of metabolites of ICT2700 was also explored using glutathione as a nucleophile. The metabolites were identified by a combination of LC/MS and LC MS/MS techniques. Investigations using mouse and human liver microsomes show that a large number of metabolites are created though none were shown to be associated with a potential anticancer effect. Studies focused on CYP2W1 show that this isoform metabolised ICT2706 to a cytotoxic species and a pharmacokinetic study showed a good distribution of ICT2706 into mouse tissues including tumour. However metabolism of ICT2726 by CYP2W1 resulted only in a non-toxic metabolite profile and may have potential as a biomarker for functional CYP2W1 in tissues. Preliminary studies show that palmitic acid hydroxylation is a useful marker of functional CYP4F11. Summary and conclusion The in vitro results show that the chloromethylindolines are a novel class of agent with potential as prodrugs that following specific hydroxylation by CYP1A1 and CYP2W1 are converted to ultra-potent cytotoxins. Other metabolites are also evident which are not cytotoxic. Studies in vivo show that selected chloromethylindolines possess a good pharmacokinetic profile and show potential as prodrug anticancer agents that require activation by CYP1A1 or CYP2W1. The methods, results, progress and suggestions for future work are presented in this thesis.

APA, Harvard, Vancouver, ISO, and other styles

5

Alandas, Mohammed Nasser. "An investigation into the metabolic activation of novel chloromethylindolines by isoforms of cytochrome P450 : targeting drug metabolising enzymes in cancer : analysis of the role and function of selected cytochrome P450 oxidising novel cancer prodrugs." Thesis, University of Bradford, 2012. http://hdl.handle.net/10454/6289.

Full text

Abstract:

Introduction: Cytochromes P450 (CYPs) are the major family of enzymes responsible for detoxification and metabolism of a wide range of both endogenous and xenobiotics chemicals in living organisms. The use of CYPs to activate prodrugs to cytotoxins selectively in tumours has been explored including AQ4N, Phortress and Aminoflavone. CYP1A1, CYP1B1, CYP2W1, and CYP4F11 have been identified as expressed in tumour tissue and surrounding stroma at high frequency compared to most normal tissues. Aim is to investigate the differential metabolism of novel chloromethylindoline by high frequency expressed CYPs in tumours. This differential may be exploited to elicit a selective chemotherapeutic effect by metabolising inert small molecules to potent cytotoxins within the tumour environment. Materials and Methods: Sensitive and specific LC/MS/MS techniques have been developed to investigate the metabolism of chloromethylindolines. Recombinant enzymes and transfected cell lines were used to investigate the metabolic profiles with a focus on production of the cytotoxic derivatives of chloromethylindolines. Results: Detailed metabolic studies show that (1-(Chloromethyl)-1,2-dihydropyrrolo [3,2-e]indol-3(6H)-yl)(5-methoxy-1H-indol-2-yl) methanone (ICT2700) and other chloromethylindolines are converted by CYP1A1 mediated hydroxylation at the C-5 position leading to highly potent metabolites. In vitro cytotoxicity studies showed differentials of up to 1000-fold was achieved between CYP1A1 activated compared to the non-metabolised parent molecules. The reactivity of metabolites of ICT2700 was also explored using glutathione as a nucleophile. The metabolites were identified by a combination of LC/MS and LC MS/MS techniques. Investigations using mouse and human liver microsomes show that a large number of metabolites are created though none were shown to be associated with a potential anticancer effect. Studies focused on CYP2W1 show that this isoform metabolised ICT2706 to a cytotoxic species and a pharmacokinetic study showed a good distribution of ICT2706 into mouse tissues including tumour. However metabolism of ICT2726 by CYP2W1 resulted only in a non-toxic metabolite profile and may have potential as a biomarker for functional CYP2W1 in tissues. Preliminary studies show that palmitic acid hydroxylation is a useful marker of functional CYP4F11. Summary and conclusion: The in vitro results show that the chloromethylindolines are a novel class of agent with potential as prodrugs that following specific hydroxylation by CYP1A1 and CYP2W1 are converted to ultra-potent cytotoxins. Other metabolites are also evident which are not cytotoxic. Studies in vivo show that selected chloromethylindolines possess a good pharmacokinetic profile and show potential as prodrug anticancer agents that require activation by CYP1A1 or CYP2W1. The methods, results, progress and suggestions for future work are presented in this thesis.

APA, Harvard, Vancouver, ISO, and other styles

6

Hyman, M. R. "Organic substrates for the ammonia oxidising enzyme of Nitrosomonas europaea." Thesis, University of Bristol, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.354458.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Oxidising enzymes"

1

Wichers, H. J., and C. Boeriu. "Controlling the texture of fruit and vegetables: the role of oxidising enzymes." In Texture in Food, 295–320. Elsevier, 2004. http://dx.doi.org/10.1533/978185538362.3.295.

Full text

APA, Harvard, Vancouver, ISO, and other styles

We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!