Dissertations / Theses on the topic 'Oxidative state of protein'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Oxidative state of protein.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Tokarew, Jacqueline M. "A Novel Role for the Parkinson Disease-Linked and Neuromelanin-Associated Parkin Protein as a Cysteine-Dependent Redox-State Regulator." Thesis, Université d'Ottawa / University of Ottawa, 2021. http://hdl.handle.net/10393/42389.
Full textLui, James Kwok Ching. "A fluorescent labelling technique to detect changes in the thiol redox state of proteins following mild oxidative stress." University of Western Australia. School of Biomedical, Biomolecular and Chemical Sciences, 2008. http://theses.library.uwa.edu.au/adt-WU2008.0056.
Full textGuo, Liang. "Structural and functional studies of mitochondrial small Tim proteins." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/structural-and-functional-studies-of-mitochondrial-small-tim-proteins(03dde6fd-6692-4af5-9023-b85a33803fcd).html.
Full textMaxwell, Brian Andrew. "Multi-disciplinary Investigation of the Kinetics and Protein Conformational Dynamics of DNA Replication and Oxidative DNA Damage Bypass and Repair." The Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1405961617.
Full textMensah, Eric. "Creation of a Site-Directed Mutant of Hen Egg White Lysozyme Working Toward Site-Specific Oxidation as it Relates to Protein Structure." Connect to resource online, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ysu1251756763.
Full textBilous, I. I. "The dynamics of the parameters of lipid peroxidation, the oxidative modification of proteins and the state of the blood antioxidant system 3 and 6 months after treating diabetic polyneuropathy." Thesis, БДМУ, 2017. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/17030.
Full textBilous, Iryna Ivanivna. "The dynamics of the parameters of lipid peroxidation, the oxidative modification of proteins and the state of the blood antioxidant system 3 and 6 months after treating diabetic polyneuropathy." Thesis, ВДНЗ України "Буковинський державний медичний уніврситет", 2017. http://dspace.bsmu.edu.ua:8080/xmlui/handle/123456789/14083.
Full textTuckey, Nicholas Pierre Lemieux. "Technologies for tissue preservation: the role of endogenous and exogenous antioxidants in preserving tissue function in chinook salmon, Oncorhynchus tshawytscha." Thesis, University of Canterbury. Biological Sciences, 2008. http://hdl.handle.net/10092/1510.
Full textDu, Aiguo. "Prediction of oxidation states of cysteines and disulphide bridges in proteins." unrestricted, 2007. http://etd.gsu.edu/theses/available/etd-11272007-024411/.
Full textTitle from file title page. Y. Pan, committee chair; G. Qin, A. Bourgeois, A. Zelikovski, committee members. Electronic text (124 p. : ill. (some col.)) : digital, PDF file. Description based on contents viewed June 3, 2008. Includes bibliographical references (p. 111-124).
Harvey, Anna Ross. "Oxidative protein folding in Aspergillus niger." Thesis, University of Nottingham, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.523081.
Full textXiao, Ruoyu. "Protein disulfide isomerase : function and mechanism in oxidative protein folding /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-238-1/.
Full textDu, Aiguo. "Prediction of Oxidation States of Cysteines and Disulphide Connectivity." Digital Archive @ GSU, 2007. http://digitalarchive.gsu.edu/cs_diss/28.
Full textHammerman, Malin. "Oxidative Stress and Protein Acetylation in Adipocytes." Thesis, Linköpings universitet, Proteinkemi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-75785.
Full textCosta, N. J. "Mitochondrial protein thiol modifications during oxidative stress." Thesis, University of Cambridge, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598052.
Full textCÌŒemazar, Masa. "Oxidative folding of a cystine knot protein." Thesis, Open University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.275108.
Full textAnnangudi, Palani Suresh Babu. "Lipid-based Oxidative Protein Modifications in Glaucoma." Case Western Reserve University School of Graduate Studies / OhioLINK, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=case1129558048.
Full textGrundy, Nicholas Matthew. "Protein S-thiolation and oxidative stress in plants." Thesis, Durham University, 2002. http://etheses.dur.ac.uk/3950/.
Full textCarr, M. D. "NMR studies of oxidative phosphorylation." Thesis, University of Oxford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382584.
Full textTan, Yew-Foon. "Metal-protein interactome in plant mitochondria." University of Western Australia. School of Biomedical, Biomolecular and Chemical Sciences, 2009. http://theses.library.uwa.edu.au/adt-WU2009.0162.
Full textLuca, Corneliu Constantin. "MTERFD3 is a Mitochondrial Protein that Modulates Oxidative Phosphorylation." Scholarly Repository, 2008. http://scholarlyrepository.miami.edu/oa_dissertations/132.
Full textClements, Casey M. Ting Jenny P. Y. "Functional characterization of DJ-1 an oxidative response protein /." Chapel Hill, N.C. : University of North Carolina at Chapel Hill, 2007. http://dc.lib.unc.edu/u?/etd,1218.
Full textTitle from electronic title page (viewed Mar. 26, 2008). "... in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Microbiology and Immunology." Discipline: Microbiology and Immunology; Department/School: Medicine.
Matsusaki, Motonori. "Molecular Mechanism of Oxidative Protein Folding by Soybean Protein Thiol Disulfide Oxidoreductases/ERO1 Pathway." Doctoral thesis, Kyoto University, 2016. http://hdl.handle.net/2433/217183.
Full text0048
新制・課程博士
博士(農学)
甲第20008号
農博第2192号
新制||農||1045(附属図書館)
学位論文||H28||N5017(農学部図書室)
33104
京都大学大学院農学研究科農学専攻
(主査)教授 裏出 令子, 教授 松村 康生, 教授 三上 文三
学位規則第4条第1項該当
Doctor of Agricultural Science
Kyoto University
DFAM
Matsusaki, Motonori. "Molecular Mechanism of Oxidative Protein Folding by Soybean Protein Thiol Disulfide Oxidoreductases / ERO1 Pathway." Kyoto University, 2009. http://hdl.handle.net/2433/217183.
Full text0048
新制・課程博士
博士(農学)
甲第20008号
農博第2192号
新制||農||1045(附属図書館)
学位論文||H28||N5017(農学部図書室)
33104
京都大学大学院農学研究科農学専攻
(主査)教授 裏出 令子, 教授 松村 康生, 教授 三上 文三
学位規則第4条第1項該当
Gregory, Mary Sarah-Jane, and n/a. "Thioredoxin and Oxidative Stress." Griffith University. School of Health Science, 2004. http://www4.gu.edu.au:8080/adt-root/public/adt-QGU20040301.082639.
Full textGregory, Mary Sarah-Jane. "Thioredoxin and Oxidative Stress." Thesis, Griffith University, 2004. http://hdl.handle.net/10072/367183.
Full textThesis (Masters)
Master of Philosophy (MPhil)
School of Health Sciences
Full Text
Karamullaoglu, Gulsun. "Dynamic And Steady-state Analysis Of Oxidative Dehydrogenation Of Ethane." Phd thesis, METU, 2005. http://etd.lib.metu.edu.tr/upload/12606269/index.pdf.
Full textand the small Cr2O3 and V2O4 phases of Cr-V-O were revealed. In H2-TPR, both catalysts showed reduction behaviour. From XPS the likely presence of Cr+6 on fresh Cr-O was found. On Cr-V-O, the possible reduction of V+5 and Cr+6 forms of the fresh sample to V+4, V+3 and Cr+3 states by TPR was discovered through XPS. With an O2/C2H6 feed ratio of 0.17, Cr-O exhibited the highest total conversion value of about 0.20 at 447°
C with an ethylene selectivity of 0.82. Maximum ethylene selectivity with Cr-O was obtained as 0.91 at 250°
C. An ethylene selectivity of 0.93 was reached with the Cr-V-O at 400°
C. In the experiments performed by using CO2 as the mild oxidant, a yield value of 0.15 was achieved at 449°
C on Cr-O catalyst. In dynamic experiments performed over Cr-O, with C2H6 pulses injected into O2-He flow, the possible occurrence of two reaction sites for the formation of CO2 and H2O was detected. By Gaussian fits to H2O curves, the presence of at least three production ways was thought to be probable. Different from Cr-O, no CO2 formation was observed on Cr-V-O during pulsing C2H6 to O2-He flow. In the runs performed by O2 pulses into C2H6-He flow over Cr-V-O, formation of CO rather than C2H4 was favored.
Gonzalez, Veronica. "The role of protein disulfide isomerase (PDI) in oxidative folding." To access this resource online via ProQuest Dissertations and Theses @ UTEP, 2008. http://0-proquest.umi.com.lib.utep.edu/login?COPT=REJTPTU0YmImSU5UPTAmVkVSPTI=&clientId=2515.
Full textMcKenna, Tomás. "Oxidative stress on mammalian cell cultures during recombinant protein expression." Licentiate thesis, Linköping University, Linköping University, Biotechnology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-51823.
Full textWhen the cell is under stress arising from oxidation, heat, infection, toxic contamination or any other stressful condition, proteins may unfold and expose residues in their structure that under normal physiological conditions are hidden and shielded from chemical reactions.
In this licentiate thesis the effects of general oxidative stress on the production of recombinant protein by mammalian cells are considered.
The work consisted of a broad literary review focused on oxidative stress and cellular response, cross-protection, gene regulation in response to oxidative stress and the relevance of this to pharmaceutical industry. A series of oxidative stressors is described and examined for experimental use. Experimental cultivation and maintenance of several mammalian cell lines was performed and several candidate stressing agents were proven on these cell lines. Menadione was selected as a powerful and consistent stressing agent, and so several experiments were performed where batches of cells were exposed to varying degrees of stress.
The performance of the cells in regard to production of recombinant protein was then examined by ELISA, showing a strong downregulation of production under stressful conditions. Recombinant protein taken from stressed and control cultures is then isolated, purified and examined with MALDI-TOF spectrometry. Finally mRNA from the cells is isolated and examined by means of microarray. Genes that are significantly regulated are examined, and those genes that may have significance in the area of stress regulation and reaction are listed.
The results of the study show that mitomycin C exerts oxidative stress on the industrial protein expressing mammalian cell lines tested.
Basoah, Afua. "The effect of oxidative stress on protein modification and degradation." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.400552.
Full textScheinost, Johanna C. "A cholesterol oxidative metabolite and its role in protein misfolding." Thesis, University of Oxford, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.504523.
Full textAuciello, Francesca Romana. "Canonical and non-canonical regulation of AMP-activated protein kinase." Thesis, University of Dundee, 2015. https://discovery.dundee.ac.uk/en/studentTheses/2720a2b7-3f1e-445c-b008-c5c235f35395.
Full textDunner, Emily M. "Defective Signal Transduction and Oxidative Stress in Ataxia-Telangiectasia." Thesis, Griffith University, 2007. http://hdl.handle.net/10072/367278.
Full textThesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Biomolecular and Biomedical Sciences
Science, Environment, Engineering and Technology
Full Text
Zhou, Deyu. "Learning the hidden vector state model for extracting protein-protein interactions." Thesis, University of Reading, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.515744.
Full textWang, Wenzhong. "Mechanistic studies of flavoenzymes in fatty acid oxidation and oxidative protein folding." Access to citation, abstract and download form provided by ProQuest Information and Learning Company; downloadable PDF file, 233 p, 2007. http://proquest.umi.com/pqdweb?did=1362529911&sid=3&Fmt=2&clientId=8331&RQT=309&VName=PQD.
Full textLee, Julie 1983. "Role of oxidative stress in the regulation of iron regulatory protein 2." Thesis, McGill University, 2008. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=116073.
Full textFrand, Alison R. (Alison Renee) 1971. "The role of ERO1 in oxidative protein folding in the endoplasmic reticulum." Thesis, Massachusetts Institute of Technology, 1999. http://hdl.handle.net/1721.1/9361.
Full textIncludes bibliographical references.
The formation of native disulfide bonds is critical for the folding and stability of many secreted proteins. We describe an essential S. cerevisiae gene, ER01, which encodes a conserved ER membrane protein required for disulfide bond formation in the er .doplasmic reticulum (ER). In a conditional ero 1-1 mutant, secretory proteins that would normally contain disulfide bonds, such as carboxypeptidase Y (CPY), are retained in the ER in reduced form, as shown by thiol modification with AMS. ER01 levels determine cellular oxidizing capacity, since mutation of ER01 causes hypersensitivil/ to the reductant OTT, whereas overexpression of ER01 confers resistance to OTT. Moreover, the thiol oxidant diamide can restore growth and secretion to ero1 mutants. These results suggest that Ero1p provides the oxidizing equivalents utilized for disulfide bond formation in the ER. Oxidizing equivalents are transferred directly from Ero1p to the abundant ER oxidoreductase PDI (protein disulfide isomerase) and its homolog Mpd2p. PDI is oxidized in wild-type cells, but reduced in the ero 1-1 mutant. Thiol-disulfide exchange between POI and Ero1p is indicated by the capture of PD1-Ero1p mixed-disulfides. PDI oxidizes secretory proteins, since newly-synthesized CPY remains fully reduced in POI-depleted cells. Mixed-disulfides between PDI and p1 CPY are also detected, indicating that PDI engages directly in thiol-disulfide exchange with this substrate. Together, these results define a pathway for protein disulfide bond formation in the ER wherein oxidizing equivalents flow from Ero1p to POI (and Mpd2p) and then to substrate proteins through direct thiol-disulfide exchange reactions. Oxidized glutathione (GSSG) does not serve as an obligate intermediate In this pathway, since oxidative protein folding proceeds normally in a gsh 1.1 mutant devoid of intracellular glutathione. Mutational analysis of ER01 identifies two pairs of conserved, vlclnal cystelnes essential for Ero1p function. Mutation of Cys100, Cys105, Cys352, or Cys355 of Ero1 p disrupts cell viability, CPY folding, and thiol-disulfide exchange between POI and Ero1p. Cys100 of Ero1p may be preferentially attacked by POI, while the Cys352- Cys355 disulfide may re-oxidize the Cys 100-Cys 105 cystelne pair. The properties of yeast Ero1 p resemble those of E. coli DsbB.
by Alison R. Frand.
Ph.D.
Shute, Max. "Effect of Whey Protein Isolate on Oxidative Stress, Exercise Performance, and Immunity." Diss., Virginia Tech, 2004. http://hdl.handle.net/10919/11113.
Full textPh. D.
Weids, Alan. "Protein aggregation, oxidative stress and the role of the yeast peroxiredoxin Tsa1." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/protein-aggregation-oxidative-stress-and-the-role-of-the-yeast-peroxiredoxin-tsa1(742029c5-9b7e-47f6-b0e9-2f396b4af52a).html.
Full textBader, Martin. "Elucidation of electron transfer pathways during oxidative protein folding in Escherichia coli /." [S.l. : s.n.], 2001. http://www.bsz-bw.de/cgi-bin/xvms.cgi?SWB9167722.
Full textLiu, Quan. "PHOSPHORYLATION AND SEQUENCE DEPENDENCY OF NEUROFILAMENT PROTEIN OXIDATIVE MODIFICATION IN ALZHEIMER DISEASE." Connect to text online, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=case1102024839.
Full textZhu, Donghui. "Effects of oxidative stress and Alzheimer's amyloid-beta peptide on astrocytes." Diss., Columbia, Mo. : University of Missouri-Columbia, 2006. http://hdl.handle.net/10355/5900.
Full textThe entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file viewed on (March 3, 2007) Vita. Includes bibliographical references.
Liu, Fenglong. "Calcium-dependent protein kinase regulates soybean serine acetyltransferase in response to oxidative stress." [Gainesville, Fla.]: University of Florida, 2002. http://purl.fcla.edu/fcla/etd/UFE0000561.
Full textYi, Dong-Hui Chemistry Faculty of Science UNSW. "The Study of Biomarkers of Protein Oxidative Damage and Aging by Mass Spectrometry." Awarded by:University of New South Wales. School of Chemistry, 1999. http://handle.unsw.edu.au/1959.4/17636.
Full textLi, Shengchun. "Antioxidant systems and protein phosphatases in metabolic and signaling responses to oxidative stress." Thesis, Paris 11, 2013. http://www.theses.fr/2013PA112086.
Full textOxidative stress is a key player in plant responses to challenging environmental conditions. The intricate nature of the regulation of cellular redox state means that much remains to be elucidated on interactions between different components in these conditions. By using a genetic approach based on a catalase-deficient Arabidopsis mutant (cat2) that presents well-defined, predictable changes in redox state, this study explored interactions between oxidative stress and (1) a specific gene involved in protein dephosphorylation, and (2) specific enzymes involved in the antioxidative/reducing system. The results showed that protein phosphatase 2 subunit B'γ (PP2A-B'γ) is involved in determining day length-dependent phenotypes and related defense responses in cat2. A cat2 pp2A-B'γ double mutant showed a range of responses that were not observed in cat2 grown in short days, including lesion formation and accumulation of salicylic acid (SA) and related metabolites. Metabolomics and proteomics analyses showed that these effects were associated with altered abundance of specific metabolites and proteins, as well as changes in protein phosphorylation status. A second part of the study investigated the importance of NADP-generating enzymes in oxidative stress by production of cat2 nadp-me2 double mutants, in which the cytosolic isoform of NADP-malic enzyme is knocked out. Although NADP-ME2 was shown to be induced by oxidative stress, and mutants for this gene had much decreased leaf NADP-malic enzyme activity, no effects on cat2 phenotypes or redox profiles were apparent. Similarly, phenotypic responses to ozone were not affected in an nadp-me2 single mutant. In the third part, coupling between ascorbate and glutathione pools during oxidative stress was investigated by introduction of loss of function mutations for dehydroascorbate reductase (DHAR) into the cat2 background. In lines carrying a combination of dhar1 and dhar3 mutations, extractable leaf activity was decreased to very low levels. Despite this, cat2 dhar1 dhar3 and cat2 phenotypes and ascorbate and glutathione pools were similar. However, preliminary functional analysis of a cat2 dhar1 dhar2 dhar3 quadruple mutant suggested that the three DHARs play functionally redundant roles in oxidative stress. Overall, the work provides new data on enzymes that regulate responses to oxidative stress and has produced interesting genetic tools for further study
Okuda, Aya. "Novel Soybean Enzymes Involved in the Oxidative Protein Folding in the Endoplasmic Reticulum." 京都大学 (Kyoto University), 2017. http://hdl.handle.net/2433/225656.
Full textDrießen, Marc D. [Verfasser]. "Investigation of nanoparticle toxicity: Characterization of protein corona and evaluation of oxidative stress by protein carbonylation / Marc D. Drießen." Berlin : Freie Universität Berlin, 2016. http://d-nb.info/1111558787/34.
Full textLohman, Jeremy R. "Two-state conformational behavior in protein active centers /." Connect to title online (ProQuest) Connect to title online (Scholars' Bank), 2007. http://hdl.handle.net/1794/6198.
Full textTypescript. Includes vita and abstract. Includes bibliographical references (leaves 74-82). Also available online in Scholars' Bank; and in ProQuest, free to University of Oregon users. Also available online.
Lohman, Jeremy R. 1981. "Two-state conformational behavior in protein active centers." Thesis, University of Oregon, 2007. http://hdl.handle.net/1794/6198.
Full textCellular processes are carried out by proteins, which often utilize conformational changes for function. In theory, conformational changes can be harnessed to promote, prevent or monitor cellular processes. Such changes in protein active centers require perturbations through interactions with other proteins, small molecules or through energy input into the system, for example light. The work presented incorporates rational design and crystallographic elucidation of two-state conformational changes in two proteins, green fluorescent protein (GFP) and malate synthase (MS). GFP indicators were previously developed to quantitate the thiol/disulfide redox status within cells. Cysteine residues were introduced in close proximity on the surface of GFP and allow the formation of a disulfide bond. The indicators provide a fluorescent readout of the ambient thiol/disulfide equilibrium, however thermodynamic studies showed the resulting thiol/disulfide to be unusually stable (-287 mV) in comparison to the cellular redox buffer glutathione (-240 mV). In order to produce a family of redox indicators suitable for use in less reducing environments, amino acids were inserted near the introduced cysteine pair in order to destabilize the disulfide. The resulting family of redox indicators, termed roGFP-iX, exhibit midpoint potentials in the more desirable range of -229 to -246 mV. Crystallographic analysis indicates that roGFP-iX indicators undergo much larger two-state conformational changes than the original indicators. Surprisingly, a cis-peptide was discovered between the cysteine and the inserted residue which in combination with the conformational changes helps to explain the reduced stability of the disulfide. Malate synthase is an important virulence factor for certain microbes and carries out the Claisen condensation between glyoxylate and acctyl-CoA to produce malate. Crystal structures of Mycobacterium tuberculosis and Escherichia coli malate synthase isoform G had previously been determined with substrates or products bound. To determine the conformational changes necessary for substrate binding and product release, crystal structures of Escherichia coli malate synthase isoform A were determined in both the apo and acetyl-CoA/inhibitor bound forms. The crystallographic models revealed two-state conformational changes in the part of the active-site loop necessary for substrate binding, which has important implications for drug design. This dissertation includes my unpublished co-authored materials.
Adviser: S. James Remington
Milner, Steven John. "The oxidative folding of insulin-like growth factor-I analogues /." Title page, table of contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09phm65945.pdf.
Full textUgur, Zafer. "Mass Spectroscopic Identification and Quantification of Protein Carbonyls." VCU Scholars Compass, 2012. http://scholarscompass.vcu.edu/etd/386.
Full text