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Drake, Jeremy. "The expression and function of secreted frizzled-related protein 4 in human serous ovarian carcinoma /." Connect to this title, 2006. http://theses.library.uwa.edu.au/adt-WU2007.0072.
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Lam, Bing-shing. "Genetic study of borderline and invasive ovarian cancer." Hong Kong : University of Hong Kong, 2001. http://sunzi.lib.hku.hk/hkuto/record.jsp?B23668106.
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Siebert, Tiago Henrique Rodrigues [UNESP]. "Dinâmica e imuno-histoquímica da vitelogênese durante processo de maturação ovariana no camarão Xiphopenaeus kroyeri (Heller, 1862)." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/143012.
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O camarão-sete-barbas Xiphopenaeus kroyeri está entre as espécies de crustáceos mais importantes do litoral brasileiro, com destaque social, econômico, ambiental e cultural. Além disso, destaca-se a ampla distribuição geográfica no litoral Atlântico Ocidental (Rio Grande de Sul - BR até a Carolina do Norte - EUA). Também evidencia-se o aumento da captura, com consequente diminuição dos estoques naturais. A falta de conhecimento sobre a biologia das espécies, principalmente aquelas visadas pelas frotas pesqueiras, diminui a eficiência de aplicações de medidas para proteção dos estoques naturais. O conhecimento sobre a dinâmica reprodutiva de uma espécie pode ser considerado importante e eficaz para controlar uma população explorada. Com relação aos crustáceos, o ciclo reprodutivo apresenta ovários em diferentes estágios de maturação e a determinação destes estágios envolve as atividades inerentes ao ovário e ao hepatopâncreas. Estes dois órgãos trabalham de forma sincronizada durante a vitelogênese. Assim, o objetivo deste estudo foi reavaliar alguns pontos referente a dinâmica celular em fêmeas de Xiphopenaeus kroyeri nos diferentes estágios de maturação ovariana. A classificação macroscópica e microscópica da maturação ovariana e o perfil celular dos túbulos hepatopancreáticos foram estudados através de análises histológicas e histoquímicas; a localização da síntese de vitelogenina no ovário e hepatopâncreas foi estudada através da imuno-histoquímica; e os índices gonadossomático (IGS) e hepatossomático (IHS) foram comparados. Deste modo, o ovário de X. kroyeri apresenta seis estágios de maturação: imaturo, pré-maturo, maturação inicial, maturação avançada, maduro e desovado. As células germinativas apresentam seis fases de desenvolvimento: oogônia tipo I, oogônia tipo II, oócitos pré-vitelogênicos, oócitos em vitelogênese inicial, oócitos em...
The shrimp Xiphopenaeus kroyeri is among the most important shellfish species off the Brazilian coast, with several highlights such as social, economic, environmental and cultural. In addition, we highlight the wide geographic distribution in the western Atlantic coast (Rio Grande do Sul - BR to North Carolina - USA) and also the increase in artisanal fisheries with consequent reduction of the natural stocks. The lack of knowledge about the biology of the species, especially those targeted by fishing fleets, decreases the efficiency of strategies for protection of natural stocks. Knowledge about reproductive dynamics of a species is considered important and effective to control an exploited population. Regarding the crustaceans, the reproductive cycle is composed of ovaries at different stages of maturation, and the determination of these stages involves activities related to the ovary and hepatopancreas. These two organs work synchronously during vitellogenesis. The objectives of this study were to reevaluate some points in females in different stages of ovarian maturation. Macroscopic and microscopic classification of ovarian maturation and the cellular profile of hepatopancreatic tubules were studied by histological and histochemical analyzes; the location of vitellogenin synthesis in ovary and hepatopancreas was studied by immunohistochemistry and the gonadosomatic (GSI) and hepatosomatic (IHS) indices were compared. Thus, X. kroyeri ovary features six maturity stages: immature, pre-mature, early maturation, advanced maturation, mature and spawned. The germ cells exhibit six phases of development: oogonia type I, oogonia type II, pre-vitellogenic oocytes, oocytes in early vitellogenesis, oocytes in advanced vitellogenesis and mature oocyte. The hepatopancreatic tubule is lined by a pseudostratified epithelium with five cell types, cell E (undifferentiated) cells F (fibrillar), cell R (resorptive), B cell (vesicular) and cell M ...
CNPq: 140453/2013-0
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Siebert, Tiago Henrique Rodrigues. "Dinâmica e imuno-histoquímica da vitelogênese durante processo de maturação ovariana no camarão Xiphopenaeus kroyeri (Heller, 1862) /." Jaboticabal, 2015. http://hdl.handle.net/11449/143012.
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Orientador: Irene Bastos Franceschini VicentiniBanca: Bruno César Schimming
Banca: Fernanda Antunes Alves da Costa
Banca: Luciene Patrici Papa
Banca: Maria Terezinha Siqueira Bombonato
Resumo: O camarão-sete-barbas Xiphopenaeus kroyeri está entre as espécies de crustáceos mais importantes do litoral brasileiro, com destaque social, econômico, ambiental e cultural. Além disso, destaca-se a ampla distribuição geográfica no litoral Atlântico Ocidental (Rio Grande de Sul - BR até a Carolina do Norte - EUA). Também evidencia-se o aumento da captura, com consequente diminuição dos estoques naturais. A falta de conhecimento sobre a biologia das espécies, principalmente aquelas visadas pelas frotas pesqueiras, diminui a eficiência de aplicações de medidas para proteção dos estoques naturais. O conhecimento sobre a dinâmica reprodutiva de uma espécie pode ser considerado importante e eficaz para controlar uma população explorada. Com relação aos crustáceos, o ciclo reprodutivo apresenta ovários em diferentes estágios de maturação e a determinação destes estágios envolve as atividades inerentes ao ovário e ao hepatopâncreas. Estes dois órgãos trabalham de forma sincronizada durante a vitelogênese. Assim, o objetivo deste estudo foi reavaliar alguns pontos referente a dinâmica celular em fêmeas de Xiphopenaeus kroyeri nos diferentes estágios de maturação ovariana. A classificação macroscópica e microscópica da maturação ovariana e o perfil celular dos túbulos hepatopancreáticos foram estudados através de análises histológicas e histoquímicas; a localização da síntese de vitelogenina no ovário e hepatopâncreas foi estudada através da imuno-histoquímica; e os índices gonadossomático (IGS) e hepatossomático (IHS) foram comparados. Deste modo, o ovário de X. kroyeri apresenta seis estágios de maturação: imaturo, pré-maturo, maturação inicial, maturação avançada, maduro e desovado. As células germinativas apresentam seis fases de desenvolvimento: oogônia tipo I, oogônia tipo II, oócitos pré-vitelogênicos, oócitos em vitelogênese inicial, oócitos em...
Abstract: The shrimp Xiphopenaeus kroyeri is among the most important shellfish species off the Brazilian coast, with several highlights such as social, economic, environmental and cultural. In addition, we highlight the wide geographic distribution in the western Atlantic coast (Rio Grande do Sul - BR to North Carolina - USA) and also the increase in artisanal fisheries with consequent reduction of the natural stocks. The lack of knowledge about the biology of the species, especially those targeted by fishing fleets, decreases the efficiency of strategies for protection of natural stocks. Knowledge about reproductive dynamics of a species is considered important and effective to control an exploited population. Regarding the crustaceans, the reproductive cycle is composed of ovaries at different stages of maturation, and the determination of these stages involves activities related to the ovary and hepatopancreas. These two organs work synchronously during vitellogenesis. The objectives of this study were to reevaluate some points in females in different stages of ovarian maturation. Macroscopic and microscopic classification of ovarian maturation and the cellular profile of hepatopancreatic tubules were studied by histological and histochemical analyzes; the location of vitellogenin synthesis in ovary and hepatopancreas was studied by immunohistochemistry and the gonadosomatic (GSI) and hepatosomatic (IHS) indices were compared. Thus, X. kroyeri ovary features six maturity stages: immature, pre-mature, early maturation, advanced maturation, mature and spawned. The germ cells exhibit six phases of development: oogonia type I, oogonia type II, pre-vitellogenic oocytes, oocytes in early vitellogenesis, oocytes in advanced vitellogenesis and mature oocyte. The hepatopancreatic tubule is lined by a pseudostratified epithelium with five cell types, cell E (undifferentiated) cells F (fibrillar), cell R (resorptive), B cell (vesicular) and cell M ...
Doutor
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Ratanasiri, Amornrat. "The outcome of primary treatment for ovarian cancer patients at srinagarind hospital during 1985-1989." Thesis, Hong Kong : University of Hong Kong, 1996. http://sunzi.lib.hku.hk/hkuto/record.jsp?B17538014.
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Hague, W. M. "Familial aspects of polycystic ovaries." Thesis, University of Cambridge, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.599834.
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Hao, Da Peng. "Integrated analysis of ovarian cancer :implications on tissue origin, hormone therapy and immunotherapy." Thesis, University of Macau, 2018. http://umaclib3.umac.mo/record=b3953959.
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Silva, Rafaela Nunes da [UNESP]. "Dinâmica da vitelogênese durante a maturação ovariana em Artemesia longinaris." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/116047.
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000805066_20150708.pdf: 84839 bytes, checksum: a2201d3a364ce6fbdad0c2a63c6d8ee1 (MD5)Sabe-se que em camarões peneídeos, a vitelogenina é sintetizada no ovário e hepatopâncreas, mas a contribuição relativa desses dois tecidos na síntese de vitelogenina ainda é incerta. Este processo pode diferir ainda de acordo com a espécie e estágio vitelogênico ou muda. Assim o presente estudo analisou a localização da produção de vitelogenina através de técnicas imunohistoquímicas, bem como, a quantificação do seu mRNA através do Real Time PCR em fêmeas de Artemesia longinaris em diferentes estágios de maturação ovariana (rudimentar - RU, em maturação - EM e maduro - MA). A espécie de camarão estudada apresenta altíssimo valor econômico. Assim, o estudo dos mecanismos da reprodução podem gerar dados para futuros projetos de produção em escala comercial, contribuindo com a preservação dos estoques naturais. A partir de análises moleculares confirmou-se a síntese de vitelogenina no ovário e hepatopâncreas de fêmeas de A. longinaris durante a maturação ovariana. Os níveis de expressão relativa de transcritos de vitelogenina no ovário e no hepatopâncreas durante a maturação ovariana demonstram que a dinâmica da síntese de vitelogenina é inversamente proporcional. A imunohistoquímica localizou a síntese endógena de vitelogenina nas células foliculares, nos oócitos pré-vitelogênicos e oócitos em vitelogênese inicial. E a síntese exógena de vitelogenina foi localizada nas células R dos túbulos hepatopancreáticos. Ainda, visto a grande contribuição do hepatopâncreas ao ciclo reprodutivo e às demais funções fisiológicas do animal, analisou-se o perfil celular do túbulo hepatopancreático nos estágios de maturação ovariana. Este órgão apresenta comportamento celular que se adapta ao ciclo reprodutivo em fêmeas, ou seja, o ciclo celular do hepatopâncreas além de obedecer a um ciclo digestivo é adaptado ao ciclo reprodutivo
It is known that in penaeid shrimp the vitellogenin is synthesized into the ovary and hepatopancreas, but the relative contribution of these two tissues in the synthesis of vitellogenin remains uncertain. This process differs according to the species and vitellogenesis stage or molt. Thus the present study examined the location of vitellogenin production by immunohistochemical techniques, as well as the quantification of its mRNA by Real Time PCR in female Artemesia longinaris at different stages of ovarian maturation (immature - RU, maturing EM and mature - MA). The shrimp species investigated presented a very high economic value. The study of the mechanisms of reproduction can generate data for future projects of commercial scale production contributing with the preservation of wild stocks. The molecular analyzes have confirmed the vitellogenin synthesis in the ovary and hepatopancreas of A. longinaris females during ovarian maturation. the relative expression level of vitellogenin transcripts in the ovary and the hepatopancreas during ovarian maturation show that the dynamics of vitellogenin synthesis is inversely proportional. By immunohistochemistry, it was located the endogenous synthesis of vitellogenin in follicular cells, in pre-vitellogenic oocytes and oocytes in early vitellogenesis. And the synthesis exogenous of vitellogenin was localized in cells R of hepatopancreaticos tubules. Also, given the great contribution of hepatopancreas reproductive cycle and other physiological functions of the animal, we analyzed the cellular profile of the hepatopancreatic tubule at stages of ovarian maturation. This organ has a cellular behavior that adapts to the reproductive cycle in females, in other words, the cellular cycle of hepatopancreas beyond to follow a digestive cycle is adapted to the reproductive cycle
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Silva, Rafaela Nunes da. "Dinâmica da vitelogênese durante a maturação ovariana em Artemesia longinaris /." Jaboticabal, 2014. http://hdl.handle.net/11449/116047.
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Orientador: Irene Bastos Franceschini VicentiniCoorientador: Fernanda Antunes Alves da Costa
Banca: Rosicleire Veríssimo Silveira
Banca: Luciene Patrici Papa
Resumo: Sabe-se que em camarões peneídeos, a vitelogenina é sintetizada no ovário e hepatopâncreas, mas a contribuição relativa desses dois tecidos na síntese de vitelogenina ainda é incerta. Este processo pode diferir ainda de acordo com a espécie e estágio vitelogênico ou muda. Assim o presente estudo analisou a localização da produção de vitelogenina através de técnicas imunohistoquímicas, bem como, a quantificação do seu mRNA através do Real Time PCR em fêmeas de Artemesia longinaris em diferentes estágios de maturação ovariana (rudimentar - RU, em maturação - EM e maduro - MA). A espécie de camarão estudada apresenta altíssimo valor econômico. Assim, o estudo dos mecanismos da reprodução podem gerar dados para futuros projetos de produção em escala comercial, contribuindo com a preservação dos estoques naturais. A partir de análises moleculares confirmou-se a síntese de vitelogenina no ovário e hepatopâncreas de fêmeas de A. longinaris durante a maturação ovariana. Os níveis de expressão relativa de transcritos de vitelogenina no ovário e no hepatopâncreas durante a maturação ovariana demonstram que a dinâmica da síntese de vitelogenina é inversamente proporcional. A imunohistoquímica localizou a síntese endógena de vitelogenina nas células foliculares, nos oócitos pré-vitelogênicos e oócitos em vitelogênese inicial. E a síntese exógena de vitelogenina foi localizada nas células R dos túbulos hepatopancreáticos. Ainda, visto a grande contribuição do hepatopâncreas ao ciclo reprodutivo e às demais funções fisiológicas do animal, analisou-se o perfil celular do túbulo hepatopancreático nos estágios de maturação ovariana. Este órgão apresenta comportamento celular que se adapta ao ciclo reprodutivo em fêmeas, ou seja, o ciclo celular do hepatopâncreas além de obedecer a um ciclo digestivo é adaptado ao ciclo reprodutivo
Abstract: It is known that in penaeid shrimp the vitellogenin is synthesized into the ovary and hepatopancreas, but the relative contribution of these two tissues in the synthesis of vitellogenin remains uncertain. This process differs according to the species and vitellogenesis stage or molt. Thus the present study examined the location of vitellogenin production by immunohistochemical techniques, as well as the quantification of its mRNA by Real Time PCR in female Artemesia longinaris at different stages of ovarian maturation (immature - RU, maturing EM and mature - MA). The shrimp species investigated presented a very high economic value. The study of the mechanisms of reproduction can generate data for future projects of commercial scale production contributing with the preservation of wild stocks. The molecular analyzes have confirmed the vitellogenin synthesis in the ovary and hepatopancreas of A. longinaris females during ovarian maturation. the relative expression level of vitellogenin transcripts in the ovary and the hepatopancreas during ovarian maturation show that the dynamics of vitellogenin synthesis is inversely proportional. By immunohistochemistry, it was located the endogenous synthesis of vitellogenin in follicular cells, in pre-vitellogenic oocytes and oocytes in early vitellogenesis. And the synthesis exogenous of vitellogenin was localized in cells R of hepatopancreaticos tubules. Also, given the great contribution of hepatopancreas reproductive cycle and other physiological functions of the animal, we analyzed the cellular profile of the hepatopancreatic tubule at stages of ovarian maturation. This organ has a cellular behavior that adapts to the reproductive cycle in females, in other words, the cellular cycle of hepatopancreas beyond to follow a digestive cycle is adapted to the reproductive cycle
Mestre
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Vila, Espuña Sílvia. "L'emmagatzematge intraovàric d'esperma en Helicolenus dactylopterus (Pisces: Scorpaeniformes)." Doctoral thesis, Universitat de Girona, 2010. http://hdl.handle.net/10803/7664.
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El penegal, Helicolenus dactylopterus dactylopterus (Pisces: Scorpaeniformes), és una espècie que habita profunditats d'entre els 200 i 1000 m i presenta una clara distribució batimètrica en funció de la seva talla. Presenta fecundació interna i a l'interior de l'ovari conté estructures d'emmagatzematge que permeten emmagatzemar l'esperma durant períodes de temps considerablement llargs. Les cèl·lules sexuals masculines es mantenen viables gràcies a diverses substàncies nutritives que obtenen de la bossa citoplasmàtica i de l'epiteli criptal que delimita les estructures d'emmagatzematge. Aquest epiteli és també responsable de la seva protecció. Un cop els ous han assolit la maduresa, els espermatozoides són alliberats al lumen ovàric i es dóna la fertilització. És una espècie zigòpara: allibera òvuls fecundats que han estat retinguts al tracte reproductiu femení durant un curt període de temps i, per tant, els embrions són alliberats en estadis molt primerencs de desenvolupament. Així, el penegal presenta una estratègia reproductiva evidentment eficaç.
The bluemouth, Helicolenus dactylopterus dactylopterus (Pisces: Scorpaeniformes), is a species that inhabits the seabed at depths between 200 and 1000 m and shows a clear size-dependent bathymetric distribution.
It has internal fertilization and, inside the ovary, there are storage structures that allow it to store sperm for considerably long periods of time. These male sexual cells remain viable thanks to several nutritious substances they obtain from the cytoplasmic bag and from the cryptal epithelium that delimits the storage structures. This epithelium is also responsible for their protection. Once the eggs have reached maturity, the spermatozoa are freed in the ovarian lumen and fertilization occurs. This species is zygoparous: liberation of fertilized ova, which have been retained in the female reproductive tract during a short period of time and, therefore, the embryos are freed in very early stages of development. Thus, the bluemouth exhibits an obviously effective reproductive strategy.
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Convery, Margaret M. "Androgen producing cells in polycystic ovaries." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55693.
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Sugimoto, Miki. "Studies on Cryopreservation of Mammalian Ovaries." Kyoto University, 2001. http://hdl.handle.net/2433/150806.
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林秉誠 and Bing-shing Lam. "Genetic study of borderline and invasive ovarian cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2001. http://hub.hku.hk/bib/B31970175.
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Kwok, Mon-sze. "Study of metastatic suppressing genes on ovarian carcinomas /." View the Table of Contents & Abstract, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36586328.
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Kong, Siu-hang Daniel. "Alpha pak interactive exchange factor as a prognostic and therapeutic target for ovarian cancer." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B4375658X.
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Scherer, Miriani Rosa [UNESP]. "Métodos alternativos de muda forçada para poedeiras comerciais." Universidade Estadual Paulista (UNESP), 2007. http://hdl.handle.net/11449/96641.
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scherer_mr_me_botfmvz.pdf: 181041 bytes, checksum: 323fdced019a1fe2524b9da8665d9db0 (MD5)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Universidade Estadual Paulista (UNESP)
O objetivo deste estudo foi avaliar métodos alternativos de muda forçada e compará-los ao método convencional (jejum) e ao grupo controle. Para isso realizou-se o experimento nas instalações do Setor de Avicultura da Faculdade de Medicina Veterinária e Zootecnia UNESP Botucatu SP. Foram utilizadas 480 aves da linhagem Isa Brown, com 80 semanas de idade, distribuídas em 6 tratamentos contendo 10 repetições de 8 aves por gaiola. O período experimental compreendeu período de descanso forçado com duração de 28 dias onde se avaliou os parâmetros de peso corporal, ovário, oviduto, fígado, gordura abdominal, teores de cálcio e fósforo contido na cinza do metatarso e desempenho produtivo das aves. Os tratamentos utilizados foram dietas de produção com restrição de componentes: T1 cálcio e fósforo, T2 cálcio, fósforo e sódio, T3 cálcio, fósforo, sódio e aminoácidos (metionina e lisina), T4 fornecimento de milho moído, T5 muda convencional através de jejum de 14 dias seguido de ração de produção até 28 dias e T6 grupo controle que recebeu ração de produção. Durante a muda todos os tratamentos receberam água e retirou-se o programa de luz artificial. O T5 promoveu maior perda de peso corporal que os demais tratamentos. Tais perdas repercutiram em incremento no consumo voluntário de ração de produção nas primeiras semanas após o período de jejum. O jejum permitiu uma completa regressão do trato reprodutivo das aves, que cessaram a postura no período. Os tratamentos com restrição de nutrientes e de milho, promoveram redução do peso vivo, do aparelho reprodutivo e do consumo voluntário de ração, porém não cessaram a produção de ovos.
The objective of this study was to evaluate alternative forced molting systems and compare then to the traditional system (fasting) and to the control group. The trial was carried out at the Faculdade de Veterinária e Zootecnia (UNESP-Botucatu) Poultry Section. Four hundred and lighty 488 Isa Brown line hens, 80 weeks old, were assigned to 6 treatments with 10 replicates of 8 hens per cage. The experiment comprised, a 28 day resting period during which, we evaluated live weight, ovary, oviduct, liver, abdominal fat, calcium and phosphorus in tarsus ash and production traits. Treatments were production diets with nutrient or ingredient restriction: T1 calcium and phosphorus, T2 calcium, phosphorus and sodium, T3 - calcium, phosphorus, sodium and aminoacids (lysine and methionine), T4 ground corn feeding, T5 conventional forced molting: 14 days fasting followed by production ration feeding until 28 days, T6 control group fed production ration. During the molting period all hens had access to water and the light program was suspended. TheT5 promoted larger bady weight loss than the other treatments. Such losses resulted in an increment in the voluntary intake in the first weeks after the fasting period. Fasting allowed a complete regression of the reproductive tract of the birds and interrupted laying. The ingredient restriction treatments and ground corn feeding promoted reduction of body weight, of the reproductive tract and of the voluntary feed intake. They reduced, bit they didn't interrupt the egg laying.
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Hodny, Elizabeth. "Increasing Awareness and Knowledge About Ovarian Cancer to Enhance Health Outcomes of Women." Diss., North Dakota State University, 2017. http://hdl.handle.net/10365/25962.
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Ovarian cancer is the fifth leading cause of cancer death among women in the U.S. and kills approximately 14,000 women each year (Nezhat et al., 2015). Survival increases with early diagnosis; the five-year survival rate in stage I is 90%. Symptoms are vague and common to many health diseases, which may well explain why upwards of 70% of women with ovarian cancer are diagnosed at stage III or IV (Slatnik & Duff, 2015). Preventative guidelines in the U.S. do not recommend screening for ovarian cancer in women of average risk (AAFP, 2016b; ACOG, 2011; Doubeni et al., 2016; Moyer, 2012; NCCN, 2015; Qaseem et al., 2014; Wilt et al., 2015). A lack of screening recommendations and a subtle presentation point to the need for greater healthcare professional recognition of symptoms and risk factors of ovarian cancer, which can then lead to a prompt diagnosis. While healthcare professionals have the opportunity to improve women?s health, gaps in knowledge exist related to ovarian cancer risk factors and symptom recognition (Gajjar et al., 2012). Continuing education improves healthcare professionals? performance and patient health outcomes (Cervero & Gaines, 2015). Increasing healthcare professionals? knowledge of ovarian cancer may help to detect ovarian cancer in earlier stages and enhance health outcomes of women. Based on the need for an increase in awareness and knowledge among healthcare professionals, a local ovarian cancer conference was developed and offered to healthcare professionals. The conference focused on presenting ovarian cancer risk factors and symptoms. Attendees were provided with an ovarian cancer resource for patient education. The conference was evaluated through pretests and posttests and a conference evaluation survey. Data was collected the evening of the conference with 29 attendees responding. After the conference, correct responses increased in the areas of risk factor and symptom recognition. The number of correct responses increased from 106 on the pretest to 122 on the posttest. In regards to ability to educate women about ovarian cancer, 62% of respondents indicated that they were ?very confident? in their ability.Pam Solseng Ovarian Cancer Endowment Fund
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Willis, Debbie Susan. "Insulin and follicular function in polycystic ovaries." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.484080.
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Brough, C. "Functional anatomy of the ovaries of aphids." Thesis, University of East Anglia, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303714.
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Telfer, Evelyn Elizabeth. "Factors influencing follicular development in mammalian ovaries." Thesis, University of Edinburgh, 1988. http://hdl.handle.net/1842/26993.
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The studies described in this thesis have been concerned with several aspects of follicular development in the mammalian ovary. Chapters 2, 3 6 4 deal with mathematical modelling of ovarian follicle dynamics in normal animals and comparisons with experimentally manipulated animals. Chapter 5 describes a novel aethod for estimating the clonal origin of the mouse ovarian follicle. In the final two chapters, the comparative physiology and anatomy of follicular numbers and sizes and the incidence of polyovular follicles are described for a number of species. The unifying theme of these studies is that they reveal patterns existing in follicular development and utllisation by detailed examination of one species, (CBA/ca mouse), and broadly by interspeci,fic comparisons (with relation to scaling). A detailed mathematical description of the follicular dynamics of virgin CBA/ca mice up to 98 days of age has been obtained by the application of compartmental modelling to differential follicle counts. The rates of follicle growth (migration) and death have been estimated for five ?stages of development (primordial to Graafian). The model predicts age changes in follicle growth and death rate, there being transitions in the parameters at 20 days second at 60 days. The parameters for normal animals have been compared with those of anilllals under two experimental conditions: 1) by unilateral ovariectomy at 4-2 days of age, which abruptly halves the numbers of ovarian follicles and alters the ratio of large : small follicles. 2) by blocking ovulation using progesterone implants. The dynamics of follicle growth were altered by both treatments in comparison with the controls. Follicles at all stages of development were affected by unilateral ovariectomy and differences may exist with time. The compensatory response by the remaining ovary was due to a combination of an increased preantral growth rate and a decrease in atresia at antral stages. Earlier stages of follicle development were affected this may have been incidental to the compensatory response. In progesterone treated animals follicles developed through to antral stages when they un~erwent atresia. The effects of treatment were observed at three levels of development: 1) The initiation of growth from the primordial pool, 2) Growth rate of small follicles and 3) deaths at larger stages of follicular development. Longer term observations indicated that these effects may not be constant. The modelling studies have looked at numerical changes in the follicle population with time but a greater understanding of the develomental biology of the follicle is required in order to explain the changes in growth and death rates observed. This problem has been tackled initially by studying the clonal origin of the follicular epithelium. The technique used is based on the principle that cells in females. are generally mosaic as a result of X-chromosome inactivation the use of X linked cell markers phospho-glycerate kinase-1 (PGK-1). Granulosa cells were found to be polyclonal in origin with the number of progenitor cells numbering 5 on average. Analysis of cumulus and mural granulosa cells showed that substantial cell mixing had occurred and cuaulus cells were generally founded by more than one clone. Finally, comparative studies have been conducted to look at scaling of follicle sizes and numbers and of polyovular follicles. Ovarian follicle and oocyte sizes were scaled according to body weight (ranging from .005-500Kg) using data from 22 species. Primordial and Graafian follicle sizes varied with body weight but closer correlations for the latter were obtained when the sum of the surface areas or volUiles for a preovulatory set were considered as opposed to the values for individual follicles. The numbers of nongrowtng follicles 1n reserve at young adult ages were correlated with maximum longevity of the species and related to body weight. The frequency of polyov~lar follicles varied 1n species studied and were most abundant 1n the domestic bitch. The overall incidence of polyovular folUcles 1n young bitches was 14 S, being reduced to 5~ 1n bitches at 7-11 years. The frequency of the various types of polyovular preantral folUcle varied inversely with the numbers of oocytes per follicle.
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Carvalho, Isadora Resende de. "Histomorfometria ovariana de felinos domésticos (Felis catus) em distintas faixas etárias /." Jaboticabal, 2013. http://hdl.handle.net/11449/98167.
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Orientador: Marion Burkhardt de KoivistoBanca: Wilter Ricardo Russiano Vicente
Banca: Cássia Maria Barroso Orlandi
Resumo: O objetivo desse estudo foi avaliar a histologia e morfometria de ovários de 17 gatas classificadas de acordo com as idades G1 (4-12 meses); G2 (1-6 anos) e G3 (> 6 anos). Após realização da ovariosalpingo-histerectomia os ovários foram fixados, processados rotineiramente para a inclusão em parafina e corados com Hematoxilina-eosina. Os parâmetros morfométricos analisados foram: diâmetro (μm), área (μm2) e perímetro (μm) dos folículos e respectivos oócitos de diferentes tipos de folículos. A relação entre o crescimento do folículo e do oócito foi analisada. Os procedimentos estatísticos utilizados foram ANOVA, sendo as médias comparadas pelo teste de Tukey e as medianas pelo teste de Kruskal-Wallis, seguido do teste de comparações múltiplas de Dunn (P< 0,05). Ao todo foram 1039 folículos analisados histologicamente e houve diferença significativa nos diâmetros, áreas e perímetros foliculares e oocitários de folículos primordiais e primários unilaminares. Observou-se padrão bifásico para o crescimento do folículo e do oócito. Conclui-se que as gatas jovens obtiveram maiores valores em folículos primários unilaminares em relação às idosas. Sugere-se que o "pool" de folículos pequenos remanescentes e seus respectivos oócitos diminuem com a idade e que na fase senil esses folículos são de baixa qualidade, quando comparados ao início da vida reprodutiva. Estudos com morfometria do ovário poderão auxiliar pesquisas de tecnologias reprodutivas assistidas, como por exemplo, a maturação in vitro (MIV), já que o tamanho e a qualidade dos folículos e oócitos podem afetar a MIV em felinos domésticos. Adicionalmente, esses estudos poderão auxiliar na preservação de felinos selvagens em extinção
Abstract: The aim of this study was to evaluate the histology and morphometry of ovaries of 17 cats classified according to ages G1 (4-12 months), G2 (1-6 years) and G3 (>6 years). After ovariosalpingohysterectomy the ovaries were fixed in paraformaldehyde 5% prior to embedding in paraffin and staining with haematoxylin-eosin. The morphometric parameters analyzed were: diameter (μm), area (μm2) and perimeter (μm) of follicles and oocytes from different follicles types. The relationship between the growth of the follicle and oocyte was analyzed. The statistical analysis were ANOVA and the means were compared by Tukey test and medians using the Kruskal-Wallis test followed by Dunn's multiple comparisons (P<0.05). A total of 1039 follicles were analyzed histologically and there were significant difference in follicular and oocyte diameter, area and perimeter of primordial and primary unilaminar follicles. It was observed a biphasic pattern for the growth of the follicle and oocyte. In conclusion, young queens had higher values in primary unilaminar follicles than older queens. It is suggested that the remaining pool of small follicles and their oocytes decreases with age and that follicles of older queens has low quality compared to the beginning of reproductive life. Ovarian morphology studies may assist in assisted reproductive technologies such as in vitro maturation (MIV), since it is known that the size and quality of follicles and oocytes may affect the MIV in domestic queens. Additionally, it may assist in the preservation of endangered wild felines
Mestre
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Van, der Hoek Kylie. "Ovarian macrophages and the regulation of ovarian function /." Title page, table of contents and abstract only Title page, table of contents and abstract only, 2004. http://web4.library.adelaide.edu.au/theses/09PH/09phv2282.pdf.
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Wei, Na. "Oestrogen receptor subtypes in ovarian cancer." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/HKUTO/record/B39558058.
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Kong, Siu-hang Daniel, and 江肇恒. "Alpha pak interactive exchange factor as a prognostic and therapeutic target for ovarian cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B4375658X.
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Jiang, Lili, and 蒋莉莉. "Roles of ASPP family and FBI-1 in ovarian cancer." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45458893.
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Chan, Yuen-kwong. "Study on the role of genetic and epigenetic factors in relation to the BRCA genes in epithelial ovarian cancers." Click to view the E-thesis via HKUTO, 2002. http://sunzi.lib.hku.hk/hkuto/record/B42576726.
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Niculaita, Roxana. "The role of AKT1 and IKK[beta] in ovarian cancer tumorigenesis and chemotherapeutic resistance." [Kent, Ohio] : Kent State University, 2008. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=kent1227665461.
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Khoo, Ui-soon. "Genetic susceptibility to gynaecological cancers in the Chinese population." Hong Kong : University of Hong Kong, 2002. http://sunzi.lib.hku.hk/hkuto/record.jsp?B25257365.
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Wilson, Stacey J. "The effect of heat stress on ovarian function in dairy cattle /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9842573.
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Maheshwari, Abha. "Impact of ovarian ageing on fertility." Thesis, Available from the University of Aberdeen Library and Historic Collections Digital Resources, 2009. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?application=DIGITOOL-3&owner=resourcediscovery&custom_att_2=simple_viewer&pid=56245.
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魏瑋. "非編碼 RNA 在卵巢癌差異性表達的薈萃分析." Thesis, University of Macau, 2018. http://umaclib3.umac.mo/record=b3952168.
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Elshaer, Nashwa Ahmed Ali Mohammed 1986. "Molecular keys in structural evolution of insect ovaries." Doctoral thesis, Universitat Pompeu Fabra, 2015. http://hdl.handle.net/10803/454772.
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The present work aims at establishing the first bases for studying the insect oocyte polarity in a phylogenetically basal species, the cockroach Blattella germanica. Our approach was to choose some genes that have been described as key in the establishment of oocyte polarity in Drosophila melanogaster. Among them we studied, Capicua, a HMG box transcription factor repressor in B. germanica, which is required for the establishment of dorsal-ventral patterning of eggshell and embryo. The epidermal growth factor receptor (EGFR) that is also essential for the polarity establishment in the oocyte. We study the function of Notch pathway and how the components of this pathway participate in the proper development of the oocyte. Otherwise, trying to study the relationships between Capicua and EGFR, we assess the function of Pipe in B. germanica ovary, and embryo.El presente trabajo quiere asentar las bases para estudiar la polaridad en el oocito en una especie filogenéticamente basal como la cucaracha Blattella germanica. Para ello, y basándonos en la información disponible sobre genes implicados en el establecimiento de la polaridad en Drosophila melanogaster, escogimos algunos genes clave. Hemos estudiado el factor de transcripción Capicua, que en B. germanica actúa como represor de la transcripción, y es imprescindible para la formación del eje dorso-ventral del huevo y del embrión. EGFR (epidermal growth factor receptor) que participa también en el establecimiento de la polaridad en el folículo ovárico. Estudiamos la función de la vía Notch, y como los componentes de esta vía afectan el correcto desarrollo del oocito. Además, tratando de establecer una relación entre Capicua y EGFR estudiamos la función de Pipe en el ovario y el embrión de B. germanica.
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Jääskeläinen, M. (Minna). "Apoptosis-regulating factors in developing and adult ovaries." Doctoral thesis, Oulun yliopisto, 2010. http://urn.fi/urn:isbn:9789514263477.
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Abstract
Apoptosis plays a crucial part in human ovarian function from fetal development to the end of reproductive potential. Failures in the regulation of ovarian apoptosis are associated with many pathological conditions such as premature ovarian insufficiency, infertility and cancer. The purpose of the present study was to analyze the factors regulating cell survival in human fetal and adult
ovaries.
The fetus is exposed to maternal- and placental-derived estrogens and insufficient estrogen action has destructive effects on rodent ovarian development. We detected estrogen receptors and estrogen-converting enzymes in human fetal ovaries after primordial follicle formation, indicating that estrogens participate in human fetal ovarian development, especially after folliculogenesis.
The WNT4 gene is crucial for female sexual differentiation, follicle formation and oocyte survival. We detected WNT4 in follicular cells of fetal and adult human ovaries. In addition, Wnt4- knockout mice demonstrated a dramatic loss of oocytes before birth. However, no changes were detected in protein expression patterns of common apoptosis-related proteins. The results support the possible role of WNT4 in human ovarian function and strengthen previous knowledge on the antiapoptotic role of Wnt4.
Apoptosis signaling is mediated by extracellular- and mitochondria-associated- pathways, ending in caspase cascade activation and fragmentation of cellular structures. In the present study we analyzed the expression of several apoptosis-related factors and detected TRAIL, TNF, Bcl-XL, Bok and caspase-3 in human ovaries. In addition, TRAIL was found to be a potent and rapid inducer of human granulosa tumor cell (KGN) apoptosis. Lentiviral downregulation of Bok or Bcl-XL protein expression in KGN cells also resulted in significant changes in cell vulnerability to apoptosis. The results show for the first time the spatiotemporal expression patterns of TRAIL, TNF, Bcl-XL, Bok and caspase-3 in human ovaries and suggest an important functional role of TRAIL, Bok and Bcl-XL in regulation of human ovarian apoptosis.
The present study offers novel information on the expression and function of cell survival factors in human ovaries. These new findings open possibilities for future clinical research in attempts to understand and treat ovarian diseases caused by imbalanced regulatory pathways of apoptosis.
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Webber, Lisa Jan. "Prenatal follicle development in normal and polycystic ovaries." Thesis, Imperial College London, 2005. http://hdl.handle.net/10044/1/11954.
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Alton, Michelle. "Control of the oocyte population in mouse ovaries." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81585.
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Oocyte loss and meiotic prophase progression was studied in XY sex-reversed and XO female mice, two mouse models that lack pairing between their sex chromosomes. An arrest at the pachytene stage of meiosis was not observed, nor was a significant loss of oocytes at this stage compared to normal XX control mice. Thus, it was concluded that a pairing checkpoint either does not exist in oocytes or is not as stringent as the one observed in males.The effect of mutating the pro-apoptotic Bax molecule was studied at three distinct ages corresponding to the time when female germ cells are premeiotic, in meiotic prophase, and arrested in dictyotene. Although it appeared that more germ cells were retained in the Bax homozygous mutant compared to the wild-type and heterozygous mice at 18.5 dpc, by 24.5 dpc all of the mice possessed similar numbers of germ cells. These results indicate a role for Bax in germ cell death but also support the idea that an alternative pathway can compensate for the elimination of this molecule.
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Boland, Nicola I. "Experimental investigation of follicle development in mammalian ovaries." Thesis, University of Edinburgh, 1993. http://hdl.handle.net/1842/20104.
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Follicle development in the mouse ovary has been studied using two experimental approaches: firstly, a novel culture system was designed to investigate the metabolism of individual follicles for the first time, and secondly, chimaeric mice and molecular biology techniques were employed to study follicle morphogenesis and the developmental relationships between the different ovarian cell types. Physiological studies of follicle metabolism have been limited by the absence of a suitable culture system which is capable of supporting normal follicle growth and maturation. A novel culture system was developed to provide a physiological model for studies of metabolism by individual mouse ovarian follicles. This system supports the growth of individual primary mouse follicles to Graafian stages in vitro over a period of 5 days and is the first model demonstrating apparently normal ovulation of in vitro-grown follicles in response to LH. Preantral follicles of c.180 microns diameter were micro-dissected from the ovaries of 4 week old mice using fine needles and cultured individually in 20 μl of medium under mineral oil in V-shaped wells of a microtitre plate for a period of 6 days. Medium was supplemented with 1IU/ml hFSH and 5% serum from hypogonadal (hpg/hpg) mice. After every 24 hours of culture, follicles were washed and transferred to wells containing 20 μl of fresh medium: samples of 5-10 μl were then taken from the previous well for the analysis of metabolites. During growth and ovulation in culture, follicles and oocytes were morphologically indistinguishable from those during culture, indicating that premature luteinization does not occur in this system. Approximately 60% of dissected follicles reach preovulatory sizes during culture, which is considerably greater than the maturation rate in vivo, indicating that atresia is not a pre-programmed event. Experiments demonstrated that FSH-stimulation is required for full antral development and LH-induced ovulation, confirming that FSH confers LH-responsiveness to the follicle.
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Diniz, Elmo Gomes. "Desenvolvimento morfológico dos ovários em embriões e fetos bovinos da raça Nelore /." Jaboticabal : [s.n.], 2001. http://hdl.handle.net/11449/104980.
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Orientador: Cesar Roberto EsperBanca: Vênicio José de Andrade
Banca: Marcos Silva
Banca: Joaquim Mansano Garcia
Banca: Vera Fernanda Martins Hossipian de Lima
Resumo: Pouco se sabe sobre os eventos morfológicos que ocorrem durante o desenvolvimento pré-natal das gônadas nas raças zebuinas. O objetivo deste estudo foi descrever os eventos morfológicos relacionados ao desenvolvimento pré-natal da gônada, incluindo a sua formação, identificação de células germinativas primordiais, surgimento de oogônios, oócitos e folículos em embriões e fetos da raça Nelore. Oitenta e um embriões e fetos bovinos, com idade variando de 26 a 240 dias após fecundação, foram coletados em frigoríficos. A idade dos fetos foi estimada a partir de medidas tomadas no sentido crânio-caudal e aplicadas à fórmula proposta por Rexroad et. al. (1974). O sexo foi identificado a partir de observações macroscópicas e usando a técnica do PCR (Polymerase Chain Reaction) somente quando as diferenças sexuais morfológicas não foram evidentes. Para histologia, as gônadas foram fixadas em líquido de Bouin por 24 horas. Após processamento histológico, cortes de tecido de 5mm, foram corados com hematoxilina-eosina. Os resultados mostraram que a crista gonádica se formou a partir de 29 dias após fecundação. No 34º dia, células germinativas primordiais foram identificadas. As oogônias surgiram em grande quantidade entre 50 e 100 dias e seu número reduziu drasticamente, atingindo números finais aos 140 dias. Os folículos primordiais, folículos em crescimento e antrais apareceram em média aos 95, 140 e 180 dias, respectivamente. Oogônias e folículos primordiais, de forma diferente dos folículos em crescimento, apresentaram diferenças significativas no seu diâmetro nos vários períodos estudados. Folículos antrais mostraram diâmetro médio de 96,92 l 31,07mm aos 180 dias , chegando atingir médias de1331,43 l 567,43mm aos 240 dias... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Little is known about morphological events occurring during the prenatal development of gonads in the Zebu breeds. The objective of this study was to describe the morphologic events related to the prenatal development of the gonad, including its formation, identification of primordial germinative cells, appearance of oogonia, oocytes and follicles in Nelore breed embryos and fetuses. Eighty-one bovine embryos and fetuses, with age range from 26 to 240 days following fecundation, were gathered in a local slaughter-house. The age of fetuses was estimated from measures taken in the cranium-caudal direction and applied to the formula proposed by Rexroad et. al. (1974). The sex was identified from macroscopic observations and using PCR (Polymerase Chain Reaction) technique only when the morphologic sexual differences were not evident. For histology, gonads were fixed into Bouin fluid for 24 hours. Then, 5mm-tissue cuts were stained with hematoxylin-eosin. The results showed that the gonadal ridge was developed from 29 days following fecundation. At the 34th day, primordial germ cells were identified. Oogonia arose in great quantity between 50 and 100 days and its number reduced dramatically, attaining final numbers at 140 days. The primordial follicles, growing follicles and antral follicles appeared on the average at 95, 140 and 180 days, respectively. Oogonia and primordial follicles, in a different way from growing follicles, presented significant differences in its diameter in the several periods studied. Antral follicles showed 96.92 l 31.07mm in diameter at 180 days, achieving means of 1331.43 l 567.43 mm at 240 days. The statistical analysis showed a positive and highly significant correlation (P< 0.01), between the oogonia diameter and its nucleus, as well as between the primordial and growing follicles with its oocytes and respective nuclei... (Complete abstract, access undermentioned eletronic address)
Doutor
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Drake, Jeremy. "The expression and function of secreted frizzled-related protein 4 in human serous ovarian carcinoma." University of Western Australia. School of Anatomy and Human Biology, 2007. http://theses.library.uwa.edu.au/adt-WU2007.0072.
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[Truncated abstract] Ovarian cancer is currently the leading cause of death from gynaecological malignancies in women from developed countries. Serous ovarian cancer is the most prevalent type of all ovarian cancers, with the majority diagnosed in an advanced stage where treatment efficacy is reduced and patient survival is poor. Because of this fact, the development of improved detection and treatment strategies are necessary, with much research focussing on the complex molecular pathways involved in ovarian tumour growth as one potential avenue for intervention. Apoptosis, or programmed cell death, is one such area of investigation because currently successful cancer treatments induce apoptosis in tumour cells. Molecular analysis of apoptosis in both normal tissue and tumours has established a positive relationship between increased expression of secreted frizzled-related protein 4 (SFRP4) and apoptosis, however to date, very little research has focussed on the role of this gene in the ovary . . . An examination of SFRP4 and β-catenin expression in 163 primary serous ovarian carcinomas revealed high SFRP4 expression was associated with low β-catenin expression and conversely, low SFRP4 was associated with high β-catenin expression in the majority of the ovarian tumours analysed, reinforcing the inverse relationship observed in the ovarian cell lines. A positive trend was observed between cancer stage and the expression level of these proteins, with increased SFRP4 expression and reduced β-catenin expression as cancer stage increased. Additionally, patient survival revealed a trend towards increased survival among ovarian cancer patients who had tumours expressing low levels of SFRP4. Taken together, the novel findings of this study indicate that the increased expression of SFRP4 observed in a large proportion of serous ovarian cancers is a cellular response to down-regulate the level of β-catenin, and thus an attempt to maintain cellular homeostasis by counteracting the excessive proliferating signals present in these tumour cells.
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Lekola, Khomotso Podile Molvia. "Improvement of cattle oocyte retrieval techniques and hormonal influence on in vitro embryonic development." Thesis, University of Limpopo, 2015. http://hdl.handle.net/10386/1546.
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Thesis (M. Sc. (Animal Production)) -- University of LimpopoThe objectives of this study were: 1) To determine the effect of oocyte retrieval techniques (slicing and aspiration) on the quality and quantity of cattle oocytes, 2) To evaluate the effect of different concentrations of hormones on the maturational rate of cattle oocytes selected by brilliant cresyl blue staining, 3) To evaluate fertilization rate and cleavage/embryonic development of oocytes with or without cumulus cells, and 4) To compare the effect of fresh and frozen thawed semen on the fertilization rate of cattle oocytes. In Experiment 1: oocytes were recovered from abattoir derived ovaries using slicing and aspiration. The recovered oocytes were exposed for 90 minutes to 26μM of brilliant cresyl blue (BCB) stain and classified according to the colour of their cytoplasm: BCB+ (oocytes with blue cytoplasm) and BCB- (unstained oocytes). There was no difference (P>0.05) in the quality of oocytes recovered using slicing (60.7 %) or aspiration (53.7 %) techniques. In experiment 2: The BCB selected and the non-selected immature oocytes were randomly allocated into medium 199 + 10 % fetal bovine serum (FBS) maturation media. The media was supplemented with three different concentrations of hormones as treatments (T). The T1 (0.5 μg/ml of follicle stimulating hormone (FSH), 5mg/ml of luteinizing hormone (LH) and 2 μg/ml of estradiol (E2) as the control group. Then, T2 (1 μg/ml of FSH, 6 mg/ml of LH and 2.5 μg/ml of E2) and T3 (1.5 μg/ml of FSH, 7 mg/ml of LH and 4.5 μg/ml of E2). Maturation rate of oocytes was determined by the protrusion of the first polar bodies 24 hours following in vitro maturation. Treatment 2 yielded higher (P<0.05) maturation rate for both BCB+ (65.6 %) and without BCB (60.3 %) oocytes with T1 giving lower (P<0.05) maturation rate for BCB+ (22 %) and without BCB (16 %) oocytes. However, BCB- oocytes had lower (P<0.05) polar body extrusion (3.03 %, 8.1 % and 2.2 %) for T1, T2 and T3, respectively. In Experiment 3: one group of the presumptive zygotes was denuded of cumulus cells and the other group was cultured with cumulus cells. The presumptive zygotes were in vitro cultured in SOF-BSA and changed to SOF-FBS after 48 hours. High fertilization/cleavage rate was observed in oocytes cultured with cumulus cells (29.0 %) compared to the denuded oocytes (20.0 %) for 2-4 cells stage. Day 7 blastocysts were more (P<0.05) on oocytes cultured with cumulus cells (32 %) compared to denuded oocytes (13 %). In experiment 4: The matured oocytes were fertilized using fresh and frozen thawed semen. The oocytes fertilized with frozen thawed semen obtained a better number of 2-4 cell cleavage (23 %) when compared to fresh semen (19 %). Oocytes that were fertilized with frozen thawed semen also obtained higher morula (13 %) and blastocyst (8 %) compared to fresh semen with morula (3.4 %) and blastocyst (2 %). In conclusion, immature oocytes that were exposed to BCB+ and cultured in M199 supplemented with 10 % FBS, 0.5 μg/ml of FSH, 5 mg/ml of LH and 2 μg/ml of E2 had a higher (P<0.05) number of matured oocytes (extrusion of first polar body) compared to those that were not exposed to BCB (no BCB). Oocytes that were cultured with cumulus cells yielded a higher (P<0.05) number of cleaved embryos compared to the denuded oocytes. Slicing yielded a higher (P<0.05) number of oocytes, however the quality of oocytes recovered was similar compared to those recovered by the aspiration technique (P>0.05). Oocytes fertilized with frozen thawed semen yielded higher (P<0.05) number of 2-4 cell, morula and blastocyst when compared with oocytes that were fertilized using fresh semen. Keywords: ovaries, oocytes, slicing, aspiration, COCs, BCB, polar body and cattle
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Glassberg, Andrea E. "Genetic testing for susceptibility to breast and ovarian cancer : a case study of clinical decision-making in medical genetics /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/10308.
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Pon, Yuen-lam. "Regulation of cadherins and catenins in ovarian surface epithelium and ovarian cancer." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B39634231.
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Chan, Kwan-yi Queeny. "Molecular studies on endometrial and ovarian carcinogenesis." Click to view the E-thesis via HKUTO, 2007. http://sunzi.lib.hku.hk/hkuto/record/B39634474.
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Lischinovskaya, T. A., M. L. Kogan, A. I. Dunsany, and P. Lopathov. "Morphological features of ovarian apoplexy in women of Fertile age with different volumes of hemoperitoneum." Thesis, Сумський державний університет, 2013. http://essuir.sumdu.edu.ua/handle/123456789/32117.
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Ovarian apoplexy (apoplexia ovarii) is a sudden hemorrhage in the ovarian tissue during rupture of mature follicle vessels, follicular cyst, or corpus luteum cyst, accompanied by violation of the integrity of their tissues and bleeding into the abdominal cavity. The morphological changes in the ovarian tissue that caused the development of apoplexy are often of persistent nature and are not destroyed after treatment. It creates conditions for recurrence of the disease and violations in the woman's fertility.
When you are citing the document, use the following link http://essuir.sumdu.edu.ua/handle/123456789/32117
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Wong, Hung-lai. "Gene expression study in ovary cancer." Click to view the E-thesis via HKUTO, 2008. http://sunzi.lib.hku.hk/hkuto/record/B4073819X.
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Kouadio, Ange S. "Exploring the therapeutic potential of novel molecular targeted therapies in treating human ovarian cancer." Click here for download, 2008. http://proquest.umi.com/pqdweb?did=1650501211&sid=2&Fmt=2&clientId=3260&RQT=309&VName=PQD.
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Barkley, Nicole Marie Garverick Henry Allen. "Characterization of apoptosis in the developing bovine fetal ovary association with germ cell loss /." Diss., Columbia, Mo. : University of Missouri-Columbia, 2008. http://hdl.handle.net/10355/6105.
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The entire thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file; a non-technical public abstract, appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on October 9, 2009) Thesis advisor: Dr. H. Allen Garverick. Vita. Includes bibliographical references.
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Wong, Shuk-ying Esther. "NANOG in ovarian cancer." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B42925058.
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Lai, Tsz-wan Kristi. "Genetic polymorphisms in ovarian cancer." Hong Kong : University of Hong Kong, 2002. http://sunzi.lib.hku.hk/hkuto/record.jsp?B25176493.
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Michelmore, Katherine F. "Polycystic ovaries : a population based study of young women." Thesis, University of Oxford, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312194.
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Paula, Sálua Oliveira Calil de [UNESP]. "Avaliação de biomarcadores moleculares em mulheres com câncer de ovário." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/114040.
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000800374.pdf: 4978359 bytes, checksum: 5d98453223363cd41a299c852de7bacd (MD5)Introdução: O câncer de ovário (CO) é a maior causa de morte por neoplasia ginecológica nos países desenvolvidos. Atualmente, um modelo dualístico de classificação foi proposto, sendo os tumores tipo I de baixo grau, indolentes e com mutações estáveis. Os tumores tipo II são de alto grau e mais agressivos. Além disso, na tentativa de se entender o processo de carcinogênese, vários biomarcadores têm sido estudados como as micropartículas (MPs), as citocinas e as quimiocinas. O objetivo desse estudo é a avaliação de fatores solúveis da resposta inflamatória (MPs, citocinas e quimiocinas) em mulheres com CO e compará-los com os níveis encontrados em mulheres sem malignidade e com parâmetros clínicos. Métodos: Avaliaram-se 26 mulheres com CO e 16 mulheres sem evidência de neoplasia maligna (grupo controle). Foram coletadas amostras de plasma e tecido tumoral. A avaliação dos fatores inflamatórios foi realizada por meio da dosagem de citocinas (IL-1- β, IL-2, IL-6, IL-10, IL-12, IL-17A, TNF e IFN-gama, e quimiocinas (CXCL8, CXCL-9, CXCL 10, CCL 2, CCL5) e das micropartículas (neutrófilos, leucócitos, monócitos, eritrócitos, endotélio, plaquetas e linfócitos) por citometria de fluxo/CBA (Cytometric Bead Array). As diferenças entre os grupos foram avaliadas pelos testes Kruskal- Walis ou Mann-Whitney e a sobrevida por Cox Regression. As diferenças com valor de p<0,05 foram consideradas significativas.Resultados: Não houve diferença entre os grupos em relação à idade, paridade e menopausa. No grupo de mulheres com CO, 10 (38,5%) tinham estadio I/II e 16 (61,5%) tinham estadio III/IV. Em relação ao tipo tumoral, segundo a nova classificação, 8 (30.8%) eram tipo I e 18 foram tipo II (69.2%). Citorredução ótima foi obtida em 15 (57.7%) mulheres com CO. Os valores de CA 125 foram significativamente diferentes entre os grupos. Não houve óbito em mulheres com tumores ...
Introduction : Ovarian cancer ( OC) is the leading cause of death from gynecological cancer in developed countries . Currently , a dualistic classification model was proposed .Type I tumors are low-grade , indolent and have stable mutations . Type II tumors are high grade and more aggressive . Moreover, in an attempt to understand the process of carcinogenesis , several biomarkers have been studied as microparticles (MPs ) , cytokines and chemokines. The purpose of the study was to evaluate the levels of circulating soluble biomarkers-microparticles, cytokines and chemokines- to characterize the pro-inflammatory/modulatory immune response in women with OC. The correlation between the biomarker levels and the clinico-pathological parameters were also analyzed. Methods : We evaluated 26 women with OC and 16 women without evidence of malignancy ( control group ) . Plasma samples and tumor tissue were collected . The assessment of inflammatory markers was performed by measurement of cytokine (IL -1- β , IL-2 , IL-6 , IL-10 , IL-12 , IL -17A , TNF and IFN –gamma), chemokines ( CXCL8 , CXCL -9 , CXCL 10 , CCL 2 , CCL5 ) and microparticles (neutrophils , leukocytes, monocytes, erythrocytes, endothelium , platelets and lymphocytes) by flow cytometry / CBA ( cytometric Bead Array) . Differences among groups were evaluated by Kruskal - Wallis or Mann - Whitney and survival by Cox Regression . Differences with p < 0.05 were considered significant. Results: There was no difference between groups regarding age , parity and menopause. In the group of women with OC , 10 ( 38.5 % ) were stage I / II and 16 ( 61.5 % ) were stage III / IV . Concerning tumor type , according to the new classification , 8 (30.8 % ) were type I and 18 ( 69.2 % ) were type II . Optimal cytoreduction was achieved in 15 ( 57.7 % ) women with OC . CA 125 values were significantly different between groups . There were no deaths in women with type I tumors. Stratifying by ...