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1
Kremmin, Holger. "Protein-Disassembly im Verlauf der endosomalen Prozessierung." [S.l. : s.n.], 2000. http://ArchiMeD.uni-mainz.de/pub/2001/0006/diss.pdf.
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Onda, Maki. "Studies on the Refolding Process of Ovalbumin." Kyoto University, 1999. http://hdl.handle.net/2433/157137.
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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものであるKyoto University (京都大学)
0048
新制・論文博士
博士(農学)
乙第10251号
論農博第2262号
新制||農||791(附属図書館)
学位論文||H11||N3334(農学部図書室)
UT51-99-T785
(主査)教授 廣瀬 正明, 教授 井上 國世, 教授 北畠 直文
学位規則第4条第2項該当
3
Shirai, Nobuaki. "Studies on the Folding of Heat-denatured Ovalbumin." Kyoto University, 1997. http://hdl.handle.net/2433/160881.
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本文データは平成22年度国立国会図書館の学位論文(博士)のデジタル化実施により作成された画像ファイルを基にpdf変換したものであるKyoto University (京都大学)
0048
新制・課程博士
博士(農学)
甲第6898号
農博第916号
新制||農||739(附属図書館)
学位論文||H9||N3022(農学部図書室)
UT51-97-H282
京都大学大学院農学研究科食品工学専攻
(主査)教授 安本 教傅, 教授 廣瀬 正明, 教授 佐々木 隆造
学位規則第4条第1項該当
4
Bezerra, Fernanda Carvalho. "ParticipaÃÃo dos hormÃnios tireoideanos no desenvolvimento de hiperreatividade induzida pelo desafio antigÃnico com ovalbumina em traquÃias isoladas de ratos sensibilizados." Universidade Federal do CearÃ, 2005. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=114.
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Conselho Nacional de Desenvolvimento CientÃfico e TecnolÃgicoCom o objetivo de verificar a influÃncia dos hormÃnios tireoideanos no desenvolvimento de hiperreatividade traqueal, ratos machos (200 - 250 g) eutireÃideos, hipotireÃideos (propiltiouracil [PTU] - v.o. 0,05% p/v, durante 4 semanas) ou hipertireÃideos (L- tiroxina [T4] - 0,5 mg/kg s.c., por 7 ou 9 dias) foram sensibilizados à ovalbumina (OVA) e, 14 dias depois, desafiados atravÃs da inalaÃÃo de OVA (1 mg/ml, seguida de 5 mg/ml, 15 min cada). O sacrifÃcio dos animais para a realizaÃÃo dos experimentos ocorreu 24 h apÃs o desafio antigÃnico por anestesia com hidrato de cloral (400 mg/Kg). A traquÃia isolada foi montada em cubas contendo 10 ml de Krebs-Henseleit modificado (37 oC, 5% de CO2 em O2). Foram obtidas curvas concentraÃÃo-efeito (CCE) para cloreto de potÃssio (KCl), carbacol (CCh) ou serotonina (5-HT). TambÃm foram realizadas CCE ao Ca2+ em preparaÃÃes estimuladas por KCl, CCh ou 5-HT e mantidas em soluÃÃo sem Ca2+. A reapresentaÃÃo do antÃgeno promoveu significativo aumento (p < 0,05, two-way ANOVA) da resposta mÃxima (RM) das CCE ao KCl de 0,96  0,10 gF para 1,53  0,11 gF (n = 7), ao CCh de 1,98  0,06 gF para 2,92  0,07 gF (n = 7) e à 5-HT de 1,64  0,14 gF para 2,41  0,15 (n = 6) nos tecidos obtidos de animais sensibilizados ou desafiados, respectivamente. As traquÃias tambÃm apresentaram aumento (p < 0,05, two-way ANOVA) da RM ao Ca2+ quando estimuladas com KCl de 0,54  0,06 gF para 0,86  0,07 gF (n = 6), com CCh de 1,20  0,14 gF para 1,77  0,14 gF (n = 6) ou com 5-HT de 0,59  0,10 gF para 1,15  0,05 gF (n = 6) nos tecidos obtidos de animais sensibilizados ou desafiados, respectivamente. O hipotireoidismo nÃo alterou significativamente a RM induzida por KCl e CCh, enquanto que aquela induzida pela 5-HT foi reduzida de 1,64  0,14 gF nos animais eutireÃideos para 0,34  0,07 gF nos animais hipotireÃideos (p < 0,001, two-way ANOVA). ApÃs desafio, a 5-HT produziu 0,56  0,11 gF (n = 7) no tecido hipotireÃideo (p < 0,001, two-way ANOVA). O hipotireoidismo aboliu o desenvolvimento de hiperreatividade para KCl e CCh. Ocorreu um aumento na CE50 nas CCE obtidas ao CCh de 0,49 x 10-6M para 4,65 x 10-6M (P < 0,05, two-way ANOVA ). O desafio reduziu a CE50 novamente para 1,53 x 10-6M (n = 6, p<0,05, two-way ANOVA). As traquÃias de animais hipotireÃideos desafiados apresentaram diminuiÃÃo da RM ao Ca2+ quando estimuladas com KCl, CCh e 5-HT. Ocorreu aumento na CE50 nas CCE ao Ca2+ em traquÃias desafiadas e estimuladas com CCh de 5,77 x 10-4 M para 22,50 x 10-4 M (n = 6, p<0,01 two-way ANOVA). O hipertireoidismo promoveu um significativo aumento na RM das CCE apenas ao KCl (0,96  0,10 gF no controle versus 1,58  0,15 no tecido hipertireÃideo). NÃo houve desenvolvimento de hiperreatividade apÃs o desafio antigÃnico (RM = 1,87  0,14 gF). Ocorreu diminuiÃÃo da CE50 ao CCh de 0,67 x 10-4 M no controle para 0,14 x 10-4 M apÃs tratamento com T4. Os resultados mostram que hà envolvimento dos hormÃnios tireoideanos no desenvolvimento de hiperreatividade em traquÃia de rato, induzida apÃs reapresentaÃÃo do antÃgeno a animais previamente sensibilizados.
In other to verify the influence of thyroid hormones on the tracheal hyperreactivity development, euthyroid, hypothyroid (propiltiouracil [PTU] - p.o. 0.05% w/v, 4 weeks) or hyperthyroid (L-tiroxine [T4] â 0.5 mg/kg s.c.,7 or 9 days) male rats (200 - 250 g) were sensitized to ovalbumine (OVA) and, 14 days later, challenged with OVA inhalation, (1 mg/ml, followed by 5 mg/ml, 15 min each). Animals sacrifice was carried out 24 later by means of anaesthesia with chloral hydrate (400 mg/Kg). Isolated trachea was mounted in 10 ml bath chamber filled with modified Krebs-Henseleit (37 oC, 5% de CO2 em O2). Concentration-effect curves (CEC) were carried out for potassium chloride (KCl), carbachol (CCh) or serotonin (5-HT). CEC to Ca2+ added in tissues maintained in Ca2+-free medium stimulated with KCl, CCh or 5-HT also were carried out. Antigenic challenge produced significant increase (p < 0.05, two-way ANOVA) of the maximal response (Emax) of the CCE for KCl from 0.96 Â 0.10 gF to 1.53 Â 0.11 gF (n = 7), for CCh from 1.98 Â 0.06 gF to 2.92 Â 0.07 gF (n = 7) and for 5-HT from 1.64 Â 0.14 gF to 2.41 Â 0.15 (n = 6) in tissues obtained from sensitized or challenged animals, respectively. Tracheae also showed increase on the Emax to Ca2+ (p < 0.05, two-way ANOVA) when stimulated with KCl from 0.54 Â 0.06 gF to 0.86 Â 0.07 gF (n = 6), with CCh from 1.20 Â 0.14 gF to 1.77 Â 0.14 gF (n = 6) or with 5-HT from 0.59 Â 0.10 gF to 1.15 Â 0.05 gF (n = 6) on sensitized or challenged tissues, respectively. The hypothyroidism did not modify significantly the KCl- or CCh-induced Emax, while the 5-HT-induced contractile effect was reduced from 1.64 Â 0.14 gF in euthyroid tissues to 0.34 Â 0.07 gF in hypothyroid tissues (p < 0.001, two-way ANOVA). After challenge, 5-HT produced in hypothyroid tissues a contraction corresponding to 0.56 Â 0.11 gF (n = 7, p < 0.001, two-way ANOVA). Hypothyroidism prevented hyperreactivity development for KCl and CCh. It was observed an increased EC50 value in CCE for CCh from 0.49 x 10-6M to 4.65 x 10-6 M (p < 0,05, two-way ANOVA). After challenge, CE50 value was reduced to 1.53 x 10-6 M (n = 6, p < 0,05, two-way ANOVA). Tracheae from challenged hypothyroid animals showed decreased Emax to Ca2+ when they were stimulated with KCl, CCh and 5-HT. It was observed an increased EC50 value in CCE to Ca2+ in challenged tissues stimulated with CCh from 5.77 x 10-4 M to 22.50 x 10-4 M (n = 6, p < 0.01, two-way ANOVA). Hyperthyroidism significantly increased Emax of the KCl-induced CCE (0.96 Â 0,10 gF in control versus 1.58 Â 0.15 on hyperthyroid tissue). Hyperreactivity was not showed after antigenic challenge (Emax = 1.87 Â 0.14 gF). It was observed a reduction of the EC50 value to CCh from 0.67 x 10-4 M in control to 0.14 x 10-4 M after T4 treatment. Our results show that there is a putative thyroid hormones involvement in hyperreactivity development on rat trachea, after an antigenic challenge.
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Ferreira, Stella da Silva. "Avaliação do potencial erosivo do suco de laranja modificado pela adição de caseína e ovalbumina." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/23/23134/tde-21092011-095114/.
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Nesse estudo in vitro foi avaliado o potencial erosivo do suco de laranja modificado pela adição de caseína, ovalbumina e a combinação entre elas, sobre o esmalte e a dentina humanos. Duas proteínas da dieta, 0.2 g/l de caseína (CAS), 2.0 g/l de ovalbumina (OVA) e a combinação entre elas (CAS + OVA) foram adicionadas a um suco de laranja disponível comercialmente. O suco de laranja sem aditivos foi utilizado como controle negativo (C-) e o suco de laranja com adição de cálcio, também disponível comercialmente, como controle positivo (C+). O potencial erosivo dos sucos experimentais foi primeiramente comparado utilizando o método do pHStat, e em seguida, através de um modelo in vitro de erosão-remineralização. 55 espécimes de esmalte e 55 de dentina radicular (4 x 4 x 2mm) foram obtidos e incluídos em um bloco de resina acrílica. Esses blocos foram então planificados com discos de lixa abrasivos e polidos com disco de feltro e pasta diamantada. As superfícies polidas receberam a aplicação de fitas adesivas, expondo uma janela de 4 x 1mm. Os espécimes foram aleatoriamente distribuídos entre os 5 grupos experimentais (n = 11), e imersos nos respectivos sucos por 5 min, 6x ao dia, durante 5 dias. Entre as imersões e durante o período noturno, os espécimes permaneceram armazenados em saliva artificial. Após a ciclagem, os espécimes de esmalte foram analisados através de perfilometria óptica e microdureza (50 g, 15 s), enquanto que os espécimes de dentina foram analisados apenas por perfilometria óptica. Para o método do pH-Stat foi calculada a média do volume de HCl obtida em triplicata. Para a análise dos dados obtidos através de perfilometria e microdureza, foi utilizado o teste de Análise de Variância, um fator, seguido pelo teste complementar de Tukey, adotando um nível de significância de 5%. As médias do volume de HCl (em ml) obtidas no método do pH-Stat foram: C+ 0,46 (± 0,03); CAS 1,22 (± 0,06); OVA 1,10 (± 0,10); CAS+OVA 1,08 (± 0,01) e C- 1,07 (± 0,02). Na avaliação do esmalte, a perda de estrutura (m) observada foi de: C+ 0,09 (± 0,20); CAS -0,40 (± 0,32); OVA -0,44 (± 0,26); CAS+OVA -0,39 (± 0,25) e C- -1,04 (± 0,36). Quanto a microdureza superficial, os valores de dureza Knoop obtidos foram: C+ 312,68 (± 20,45); CAS 121,99 (± 10,70); OVA 108,87 (± 11,16); CAS+OVA 102,57 (± 11,89) e C- 101,94 (± 8,56). Para a dentina, a perda de estrutura observada foi de: C+ -0,82 (± 0,28); CAS -7,26 (± 0,65); OVA -6,74 (± 1,18); CAS+OVA -7,16 (± 0,75) e C- -7,51 (± 1,26). Conclui-se que para o esmalte, os sucos de laranja modificados pela adição de proteínas apresentaram um potencial erosivo reduzido. A caseína mostrou uma melhor proteção da desmineralização subsuperficial do esmalte; a sua combinação com a ovalbumina não apresentou nenhum benefício adicional. Para a dentina, nenhuma redução no potencial erosivo foi observado para os sucos de laranja modificados pela adição de proteínas.The erosive potential of a modified orange juice by addition of casein, ovalbumin and its combination, on human enamel and root dentin was evaluated in this in vitro study. Two dietary proteins, 0.2 g/l casein (CAS), 2.0 g/l ovalbumin (OVA) and their combination (CAS + OVA) were added to a commercially available orange juice. The juice with no additives was used as negative control (C-) and a commercially available calcium-modified juice as positive control (C+). The erosive potential of the experimental juices was initially compared by the pH-Stat method, and then, by an in vitro erosion-remineralization cycling model. 55 enamel and 55 root dentin specimens (4 x 4 x 2mm) were obtained and embedded in acrylic resin blocks. These blocks were ground flat with abrasive discs and polished with felt paper and diamond paste. The polished surfaces were covered with an adhesive tape, leaving a central area of 4 x 1mm exposed. The specimens were randomly allocated within the 5 experimental groups (n=11), and immersed in the respective juices for 5 min, 6x/day, for 5 days. Between the immersions and overnight they were stored in artificial saliva. After the cycling, the enamel specimens were analyzed by surface Knoop microhardness (50g, 15s) and optical profilometry, while dentin specimens were analyzed only by profilometry. The mean volume of HCl obtained in triplicate were calculated for the pH-Stat method. The data obtained for profilometry and microhardness were statistically analyzed using ANOVA, one-way, followed by Tukeys test considering a significance level of 5%. The mean volume of HCl (ml) obtained for the pH-stat method were: C+ 0,46 (± 0,03); CAS 1,22 (± 0,06); OVA 1,10 (± 0,10); CAS+OVA 1,08 (± 0,01) e C- 1,07 (± 0,02). For enamel, the surface loss (m) was: C+ 0,09 (± 0,20); CAS -0,40 (± 0,32); OVA -0,44 (± 0,26); CAS+OVA -0,39 (± 0,25) e C- -1,04 (± 0,36). Regarding microhardness, the Knoop hardness values were: C+ 312,68 (± 20,45); CAS 121,99 (± 10,70); OVA 108,87 (± 11,16); CAS+OVA 102,57 (± 11,89) e C- 101,94 (± 8,56). For dentin, the surface loss (m) was: C+ - 0,82 (± 0,28); CAS -7,26 (± 0,65); OVA -6,74 (± 1,18); CAS+OVA -7,16 (± 0,75) and C- -7,51 (± 1,26). It was concluded that protein-modified orange juices presented reduced erosive potential on enamel. Casein showed a better subsurface demineralization protection, and its combination with ovalbumin did not lead to additional benefits. For dentin, any reduction on the erosive potential was observed for protein-modified orange juices.
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Farrar, Gabrielle. "Creation of Ovalbumin Based Scaffolds for Bone Tissue Regeneration." Thesis, Virginia Tech, 2009. http://hdl.handle.net/10919/32273.
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Bio-based materials are a viable alternative to synthetic materials for tissue engineering. Although many bio-based materials have been used, Ovalbumin (OA) has not yet been researched to create 3D structures that promote cellular responses.
Micro-porous scaffolds are a promising construct for bone tissue regeneration; therefore OA crosslinked with three different concentrations (10%, 15% and 20%) of glutaraldehyde (GA) was used in this research. After fabrication, a porous morphology was observed using SEM. Average pore sizes were found to be comparable to scaffolds previously shown to promote cellular response. A TNBS assay determined percent crosslinking in the scaffolds, however there was no significant difference in percent crosslinking despite differing GA concentrations used. Possible explanations include an excess of GA was used.
Using DSC, a glass transition temperature (Tg) was found for control indicating the scaffolds are amorphous. Average dry and wet compressive strengths were also found. As expected, differing GA concentrations had no significant effect on Tg and average compressive strengths due to an excess used. Scaffolds were mechanically tested at 37°C with no significant difference found; therefore these scaffolds can be used in the body.
It was shown through cell studies that MC3T3-E1 pre-osteoblast cells significantly increased in number on the 10% and 15% scaffolds, therefore cell proliferation occurred. Because of a positive cellular response, 10% GA scaffolds were used for differentiation studies that showed an increase in osteocalcin at 21 days and alkaline phosphatase levels for scaffolds cultured for 14 days. Overall OA scaffolds have shown to be a promising 3D construct for bone tissue regeneration.Master of Science
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Yamasaki, Masayuki. "Studies on the Conformational Transition of Ovalbumin into Thermostabilized Forms." Kyoto University, 2002. http://hdl.handle.net/2433/149931.
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Kyoto University (京都大学)0048
新制・課程博士
博士(農学)
甲第9645号
農博第1273号
新制||農||847(附属図書館)
学位論文||H14||N3677(農学部図書室)
UT51-2002-G403
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 廣瀬 正明, 教授 池田 篤治, 教授 北畠 直文
学位規則第4条第1項該当
8
Matoba, Nobuyuki. "Studies on a novel anti-hypertensive peptide derived from ovalbumin." Kyoto University, 2001. http://hdl.handle.net/2433/150784.
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Kyoto University (京都大学)0048
新制・課程博士
博士(農学)
甲第9013号
農博第1195号
新制||農||822(附属図書館)
学位論文||H13||N3532(農学部図書室)
UT51-2001-F343
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 吉川 正明, 教授 佐々木 隆造, 教授 内海 成
学位規則第4条第1項該当
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Hannon, Jason Patrick. "Mechanisms of airway hyperreactivity to adenosine induced by allergen challenge in the actively sensitised brown Norway rat." Thesis, Imperial College London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312318.
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Castro, Camila Cristina de Lima. "Conjugados de ovalbumina e albumina bovina com desferrioxamina e suas interações com íons metálicos." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/46/46136/tde-21062017-091716/.
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O ferro é essencial para a vida do ser humano, desempenhando um papel fundamental no metabolismo. Contudo, quando não armazenado em compartimentos biológicos adequados, o metal apresenta um potencial tóxico ao organismo, uma vez que contribui para a formação de espécies reativas de oxigênio. A sobrecarga de ferro é uma condição desfavorável para portadores de algumas disfunções genéticas, como a hemocromatose, ou de anemias crônicas que requeiram transfusões de sangue periódicas, como é o caso da talassemia. Os fármacos atuais que controlam a patologia, como a desferrioxamina (DFO), requerem infusão subcutânea lenta, causando desconforto em pacientes e podendo trazer um série de complicações, como insuficiência hepática e renal. A modificação dessas moléculas com biopolímeros é uma proposta para minimizar efeitos colaterais e aumentar a biodisponibilidade do fármaco no organismo. Dentre esses biopolímeros, destacam-se as albuminas proveniente do soro bovino (BSA) e do ovo (OVA), que têm baixa toxicidade, baixo custo e abundância de sítios reativos, que quando modificados, favorecem reação com a desferrioxamina. Como resultado, houve a reação dos biopolímeros com a desferrioxamina, com mudanças em suas estruturas secundárias e possível dimerização, resultando na formação de conjugados possuem afinidade com íon ferro e capacidade antioxidante semelhante ao fármaco original, características que tornam os compostos bons candidatos a uma alternativa à terapia de quelação. Os conjugados BSA-DFO e OVA-DFO podem reagir, além do ferro, com gadolínio, fazendo com o que os complexos tenham uma potencial aplicação como agentes de contraste em ressonância magnética de imagem (MRI). Neste trabalho, vimos que o complexo entre Gd(III) e BSA-DFO apresentou uma relaxatividade de 52,92 s-1 mM-1 para T2 e 45,37 s-1 mM-1 para T1 , um valor bem superior aos fármacos disponíveis no mercado, que apre-sentam relaxatividade entre 4 e 5 s-1 mM-1, o que foi explicado por sua elevada massa molecular, indicando que poderia ter bons efeitos na qualidade de MRI, com menores doses.Iron is essential for human life, playing a fundamental role in metabolism. However, when not stored in appropriate biological compartments, the metal presents a toxic potential to the body, contributing to the formation of reactive oxygen species (ROS). Iron overload is an unfavorable condition for people with certain genetic disorders, such as hemochromatosis, or chronic anemias that require periodic blood transfusions, as thalassemia. Current drugs that control the pathology, as desferrioxamine, require slow subcutaneous infusion, causing discomfort in patients and may lead to a number of complications, such as hepatic and renal failures. As a result, the biopolymers were reacted with desferrioxamine, with changes in their secondary structures and possible dimerization, resulting in the formation of conjugates with iron ion affinity and antioxidant capacity similar to the original drug, characteristics that make the compounds good candidates for an alternative chelation therapy As a result, the reaction of the biopolymers with desferrioxamine caused a change in the secondary structure, with possible formation of dimers and showing different mobility when exposed to an electric potential difference. Not all polymer chains have reacted with DFO, however BSA-DFO complex has antioxidant capacity similar to the original drug. The BSA-DFO and OVA-DFO conjugates can react, in addition to iron, with gadolinium, making the complexes potential contrast agents for magnetic resonance imaging (MRI). In this work, the complex between Gd(III) and BSA-DFO presented a relaxativity of 52,92 s-1 mM-1 for T2 and 45,37 s-1 mM-1 for T1, values higher than the available drugs in the market (4 - 5 s-1 mM-1) which was explained by the high molecular weight, indicating a good effects on the quality of MRI, with lower doses.
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Wong, Ching-mang Queenie. "Structural determination of antimicrobial peptides derived from human lactoferricin and ovalbumin." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B36934732.
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Wong, Ching-mang Queenie, and 王靜萌. "Structural determination of antimicrobial peptides derived from human lactoferricin and ovalbumin." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B36934732.
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Steel, Margaret. "The regulation of systemic immune responses by the dietary antigen ovalbumin." Thesis, University of Glasgow, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.360280.
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Glaesemann, Benjamin Paul. "Ovalbumin-Based Scaffolds Reinforced with Cellulose Nanocrystals for Bone Tissue Engineering." Thesis, Virginia Tech, 2011. http://hdl.handle.net/10919/33905.
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In the field of tissue engineering, a major area of study is developing bone scaffolds that will provide support for osteoblasts. Despite many advances in recent years there is still a significant need for new bio-based 3-D porous scaffolds that possess sufficient initial mechanical properties to prevent immediate failure upon implantation. Ovalbumin (OVA), a glycoprotein from chicken egg whites, has been use to fabricate biodegradable, porous hydrogel bone scaffolds that promote osteoblast attachment and proliferation.
Although ovalbumin scaffolds encourage bioactivity and are naturally resorbed into the body after bone regeneration, they are also very fragile. Extremely stiff cellulose nanocrystals (CNCs), derived from wood pulp, can be utilized to reinforce these scaffolds while improving biocompatibility. When chemically modified to incorporate surface amine groups, cellulose nanocrystals become capable of covalently crosslinking with the OVA matrix for improved mechanical resilience.
Three concentrations (2, 5, 10 wt. %) of CNCs were incorporated and crosslinked to form nanocomposite scaffolds then were compared to pure OVA scaffolds. After fabrication, pore size morphology was compared between each CNC loading using SEM. The images revealed that the 10 wt. % CNC concentration doubled the pore compared to pure OVA scaffolds. Under high magnification, the CNCs were incorporated into the pore walls, providing a contoured surface. AFM was applied to analyze the topography of OVA with CNCs present. The surfaces laden with CNCs had a higher mean surface roughness, but was insufficient to impact cell behavior.
Compression testing was carried out on both Instron and DMA machines to demonstrate any reinforcing effect provided by the CNCs. While the compressive modulus remained constant, the elastic limit and strain increased with CNC loading, indicating a change in the resilience of the reinforced scaffolds. With a MTT Assay, it was shown that MC3T3-E1 preosteoblasts significantly increase in metabolic activity on 2 wt. % films and scaffolds, an indication of proliferation. All scaffolds had a net increase in metabolic activity suggesting overall biocompatibility for OVA scaffolds and those incorporating CNCs. Overall, the 5 wt. % scaffolds had the highest mechanical strength and had a positive cell response.Master of Science
15
Afuwape, Adeyemi Olutosin. "Oral tolerance and sensitisation : immunoregulation after feeding of ovalbumin and cow's milk." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312961.
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Lefebvre, Olivier. "Développement d’un microdispositif magnétique pour le contrôle et la détection de complexes immunologiques à base de nanoparticules magnétiques." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS537/document.
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L’objectif de cette thèse est la fabrication d’un microdispositif magnétique pour la détection et la manipulation d’éléments biologiques à base de nanoparticules magnétiques en conditions microfluidiques. Il a pour but d’intégrer des fonctions de base de contrôle et détection magnétique, pour atteindre des mesures spécifiques, stables, rapides et reproductibles. En effet, la technique d’immunodosage couplée à des nanoparticules magnétiques, bien connue dans la littérature, nécessite un contrôle du déplacement de ces dernières pour les fonctionnaliser efficacement et créer un complexe biologique encapsulant une molécule cible (biomarqueur). Dans notre cas une molécule modèle pour le domaine de la biodéfense a été utilisée : l’ovalbumine. Pour contrôler le champ magnétique nécessaire pour la capture des complexes magnétiques, nous avons opté pour l’utilisation de microbobines intégrées aux dispositifs fluidiques et comparé cette technique originale avec d’autres plus conventionnelles. Pour détecter un complexe biologique, la fluorescence est largement utilisée en biologie, mais cette technique ne permet pas une intégration complète pour un dispositif autonome. Dans cette optique, nous proposons la détection des complexes à base de nanoparticules magnétiques en relevant la variation de l’inductance d’un microcircuit magnétique refermant une chambre microfluidique contenant ces complexes immunologiques. Le dimensionnement des microbobines de contrôle par simulation a permis de déterminer les paramètres permettant d’obtenir le champ magnétique le plus adapté au contrôle des complexes biologiques. Dans le cas des microbobines utilisées pour la détection, des branches magnétiques micrométriques ont été insérées autour des microbobines pour créer un circuit de détection magnétique encore plus sensible. La réalisation de ces dispositifs a impliqué l’intégration de matériaux et de structures de nature fortement hétérogène, et leur assemblage a nécessité de résoudre de nombreux verrous technologiques. L’enjeu a été de déterminer l’ensemble des étapes successives et nécessaires pour un procédé de microfabrication fiable et reproductible. Pour montrer l’intérêt des dispositifs de capture des nanoparticules magnétiques, des tests immunologiques ont été réalisés tout d’abord en microtubes pour les comparer à ceux réalisés dans un circuit fluidique à l’aide d’aimant externe puis de microbobines intégrées. Dans ce dernier cas, une optimisation considérable a été validée en termes de réduction de temps d’incubation, de reproductibilité des mesures et de limites de détection équivalentes à l’état de l’art pour l’ovalbumine. Pour le dispositif de détection magnétique, des premières expériences de caractérisation électrique ainsi que des études en concentration de nanoparticules magnétiques ont été réalisées et comparées aux résultats obtenus par simulation. Pour la preuve de concept, un démonstrateur de détection de complexes magnétiques a été également finalisé validant la possibilité d’intégration du microcircuit magnétique dans un dispositif fluidique. Il a validé également l’obtention d’une gamme de sensibilité remarquable corrélée à la présence des complexes magnétiques. Ses caractéristiques ont été confrontées à celles obtenues par les simulations et discutées en tenant compte de toutes les étapes critiques du procédé de microfabricationRésuméThe objective of this thesis is the fabrication of a magnetic microdevice for the detection and manipulation of biological elements based on magnetic nanoparticles under microfluidic conditions. It aims to integrate basic functions of control and magnetic detection, to achieve specific, stable, fast and reproducible measurements. Indeed, the immunoassay technique coupled to magnetic nanoparticles, well known in the literature, requires a control of the displacement of magnetic nanoparticles to effectively functionalize them and create a biological complex to encapsulate a target molecule (biomarker). In our case, a model molecule for the field of biodefense was used: ovalbumin. To control the magnetic field which is necessary for the capture of magnetic complexes, we opted for the use of microcoils integrated in the fluidic devices and compared this original technique with other more conventional ones. To detect a biological complex, fluorescence is widely used in biology, but this technique does not allow a complete integration for an autonomous device. In this context, we propose the detection of complexes based on magnetic nanoparticles by observing the variation of the inductance of a magnetic microcircuit closing a microfluidic chamber containing these immunological complexes. The design of the control microcoils by simulation made it possible to determine the parameters allowing to obtain the most adapted magnetic field to the control of the biological complexes. In the case of microcoils used for detection, micrometric magnetic branches were inserted around the microcoils to create an even more sensitive magnetic sensing circuit. The realization of these devices involved the integration of materials and structures of highly heterogeneous nature, and their assembly has required to solve many technological locks. The challenge was to determine all the successive and necessary steps for a reliable and reproducible microfabrication process. To show the interest of magnetic nanoparticle capture devices, immunoassays were first performed in microtubes to compare with those made in a fluid circuit using external magnet and integrated microcoils. In the latter case, considerable optimization has been validated in terms of reduction of incubation time, reproducibility of measurements and detection limits equivalent to the state of the art for ovalbumin. For the magnetic detection device, first experiments of electrical characterization as well as concentration studies of magnetic nanoparticles were carried out and compared to the results obtained by simulation. For the proof of concept, a demonstrator of detection of magnetic complexes was also finalized validating the possibility of integration of the magnetic microcircuit in a fluidic device. It has also validated obtaining a remarkable range of sensitivity correlated with the presence of magnetic complexes. Its characteristics were compared to those obtained by the simulations and discussed taking into account all the critical steps of the microfabrication process
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Lyra, Ana Carolina Fradique de. "Avaliação da interação da ovalbumina, principal alérgeno natural da clara de ovo, com sulfonamidas através de estudos espectroscópicos e de atividade biológica." Universidade Federal de Alagoas, 2017. http://www.repositorio.ufal.br/handle/riufal/1908.
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The interaction between ovalbumin (OVA) and four sulphonamides named sulfathiazole (S1), sulfaquinoxaline (S2), sulfadimethoxine (S3) and sulfamethazine (S4) was investigated employing spectroscopic techniques (molecular fluorescence, UV-Vis and 1H NMR) emulating in natura egg conditions. For the interaction study, the intrinsic fluorescence of the protein (λex = 280 nm / λem= 336 nm) was explored in the ligand’s presence and absence (SFs). The OVA spectrometric titrations (2 μM) with different ligands (0-200 μM) were performed in pH 7.4 (Tris-HCl buffer solution 50 mM, NaCl 100 mM). For the evaluation of the interaction process, it was noticed a decrease in the OVA fluorescent emission as SFs excess was added, due to an energy transfer process (quenching). By using these data, the Stern Volmer constant (Ksv), binding constant (Kb) and stoichiometric ratio (n) were calculated. The Kb values ranged from 3.98 to 27.4 x 105 L mol-1 (30 ºC) and by applying these information, it was observed that the interaction magnitude followed the order: S3 < S4 < S2 < S1. The number of binding sites in every condition applied was close to one. For all SFs evaluated the increase in temperature lead to a decrease in both Ksv and Kb values, indicating that the static quenching mechanism is preferable, with the formation of a non-fluorescent molecular complexes. This result was further confirmed by UV-Vis studies with formation of a fundamental state complex. According to thermodynamic parameters, ΔG < 0 was obtained for all SFs evaluated, indicating that the interaction is thermodynamically spontaneous. For S2 and S3, the main forces acting in the interaction are hydrogen bonds and Van der Waals forces, whereas S1 and S4 have electrostatic forces, predominantly. Through 3D fluorescence studies, it was observed a decrease in the fluorescence intensity of peak 2 and 3, which is related to the tyrosine and tryptophan residue emissions associated with the protein secondary structure in the presence of SFs, respectively. This result indicates that there were conformational changes in the protein native structure. By using synchronized fluorescence, it was possible to deduce that the interaction with OVA exposes the tyrosine and tryptophan residues, increasing the polarity of the micro–environment of those amino acids. Through studies based on FRET (Fluorescence resonance energy transfer), it was possible to calculate the intermolecular distance between the SFs (energy acceptors) and OVA (energy donor), which were smaller than 10 nm. The competition assay with ANS probe, which control the protein hydrophobic regions, indicates that only S2 was capable to shift this marker. Metallic ions were also evaluated and it was observed that Ca(II), Mg(II) and Fe(III) can facilitate the interaction between sulphonamides (S1 and S2) and OVA. The 1H NMR studies showed a signal shift for aromatic hydrogens present in S2 and S3. In nature ovalbumin (from egg white) interacts more effectively with SFs than commercial ovalbumin. Biological studies have shown that E. coli and B. megaterium are sensitive to the sulfonamides tested and the MIC values of these antibiotics in the absence and presence of OVA do not present significant difference. This way, the results indicate that there was interaction between SFs and macromolecule and, thus, conformational changes in the protein structure were identified. So, the presence of SFs in aliments shows a risk to food security, since the OVA has its polypeptide chain modified and can act/treat as a foreigner and cause allergic reactions.Conselho Nacional de Desenvolvimento Científico e Tecnológico
A interação da ovalbumina (OVA) com quatro sulfonamidas, nomeadamente sulfatiazol (S1), sulfaquinoxalina (S2), sulfadimetoxina (S3) e sulfametazina (S4) foi investigada empregando técnicas espectroscópicas (fluorescência molecular, UV-vis e RMN 1H) simulando as condições in natura do ovo. Para o estudo de interação, explorou-se a fluorescência intrínseca da proteína (λex = 280 nm / λem = 336 nm) na ausência e presença dos ligantes (SFs). As titulações espectrofluorimétricas da OVA (2 μM) com os diferentes ligantes (0-200 μM) foram realizadas em pH 7,4 (tampão Tris 50 mM, NaCl 100 mM). Na avaliação do processo de interação notou-se diminuição na intensidade da emissão da fluorescência da OVA à medida que os excessos das SFs foram adicionados, devido ao processo de transferência de energia (quenching). Por meio destes resultados foi possível calcular a constante de Stern Volmer (Ksv), constante de ligação (Kb) e proporção estequiométrica (n). Os valores de Kb variaram de 3,98 a 27,4x105 L mol-1 (30 ºC) e a partir destes dados observou-se que a magnitude da interação seguiu a seguinte ordem: S1 > S2 > S4 > S3. O número de sítios de ligação em todas as condições foi próximo à unidade. Para todas as SFs avaliadas o aumento da temperatura levou a diminuição dos valores de Ksv e Kb, indicando que o mecanismo de quenching preferencial é estático, com formação de complexos supramoleculares não-fluorescentes. Este resultado foi confirmado pelos estudos de UV-vis com formação do complexo no estado fundamental. Quanto aos parâmetros termodinâmicos, obteve-se ΔG < 0 para todas as SFs avaliadas, indicando que a interação é termodinamicamente espontânea. Para S2 e S3, as forças predominantes na interação são ligações de hidrogênio e forças de Van der Waals, enquanto que para S1 e S4 as forças preferenciais foram eletrostáticas. Através dos estudos por fluorescência 3D observou-se a redução na intensidade de fluorescência dos picos 2 e 3, relativos à emissão dos resíduos de tirosina e triptofano e emissão associada a estrutura secundária da proteína na presença das SFs, respectivamente. Este resultado indica que houve mudanças na estrutura nativa da proteína. Por meio de fluorescência sincronizada foi possível inferir que a interação com a OVA expõe os resíduos de triptofano e tirosina aumentando a polaridade do microambiente destes aminoácidos. Através de estudos baseados em FRET (Fluorescence resonance energy transfer), foi possível calcular a distância intermolecular entre as SFs (receptoras de energia) e a OVA (doadora de energia), as quais foram inferiores a 10 nm. Os estudos de competição com a sonda ANS que monitora regiões hidrofóbicas da proteína indicaram que S2 foi a única sulfa capaz de deslocar este marcador. Já com os íons metálicos, observa-se que Ca(II), Mg(II) e Fe (III) favorecem a interação das sulfonamidas (S1 e S2) com a OVA. A partir dos estudos por ressonância magnética nuclear de hidrogênio (RMN 1H) verificou-se deslocamento dos sinais para os hidrogênios aromáticos presentes em S2 e S3. Além disto, foi verificado que a ovalbumina in natura (obtida da clara do ovo) interage com as SFs em maior magnitude quando comparado com a OVA comercial. Por meio de estudos biológicos constatou-se que a E. Coli e B. megaterium são sensíveis as sulfonamidas testadas e que os valores de MIC destes antibióticos na ausência e presença da OVA não diferem. Dessa forma, os resultados obtidos indicam que houve interação entre as SFs e a macromolécula e, consequentemente, mudanças conformacionais na estrutura da proteína foram identificadas. Logo, a presença das SFs em alimentos pode apresentar um risco a segurança alimentar, pois uma vez que há mudança conformacional na cadeia polipeptídica da OVA, esta pode comportar-se como um corpo estranho no organismo e causar/potencializar reações alérgicas.
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Ayre, Lorna M. "The adsorption of proteins onto ultrafiltration membranes." Thesis, Loughborough University, 2000. https://dspace.lboro.ac.uk/2134/35236.
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The mass of five proteins (Bovine serum albumin (BSA), casein, lysozyme, ovalbumin and pepsin) adsorbed to five different membrane materials (of various hydrophobicities) was quantified using a static system and analysed to establish any trends. Comparing the results from the five membranes it seems that there were no obvious trends between the protein masses adsorbed indicating that it may not be just one aspect of protein structure that is important in the adsorption process. Many investigations have indicated that the protein may undergo a conformational change during the adsorption process. Disulphide bridges contribute readily to the stability of the protein molecule and it was hypothesised that if such a structural change occurred, it would result in the breakage of these covalent bonds. To this end, the free thiol group content of the proteins was quantified before and after adsorption.
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Arii, Yasuhiro. "Studies on the Cellular Localization and Thermostability of Ovalbumin Produced in Escherichia coli." Kyoto University, 2000. http://hdl.handle.net/2433/78104.
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Kyoto University (京都大学)0048
新制・課程博士
博士(農学)
甲第8427号
農博第1111号
新制||農||800(附属図書館)
学位論文||H12||N3384(農学部図書室)
UT51-2000-F331
京都大学大学院農学研究科応用生命科学専攻
(主査)教授 廣瀬 正明, 教授 内海 成, 教授 北畠 直文
学位規則第4条第1項該当
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Sheets, Kevin. "Mechanical and Cellular Response to Biomineralization of Ovalbumin Scaffolds for Bone Tissue Engineering." Thesis, Virginia Tech, 2010. http://hdl.handle.net/10919/32721.
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Studies regarding the feasibility of ovalbumin (OVA) as a bone scaffold material have found its cost, availability, interaction with cells, and ability to degrade in the body into safe byproducts to be ideal for such an application. However, weak mechanical properties cause hesitation in the use of OVA as a scaffolding material in much stronger native tissue. To enhance the mechanical strength of the OVA scaffolds without compromising in vitro cellular performance, Ca-P crystals were grown on unmodified OVA and phosphonated OVA (p-OVA) samples via biomineralization processes using 5x-concentrated simulated body fluid (5x SBF).
Electron microscopy (ESEM/EDS) data confirm the formation of Ca-P crystals on the surface of OVA and p-OVA scaffolds. Mechanically, rheology data measured a minimum of a three-fold increase in each mineralized scaffoldâ s complex shear modulus over unmineralized counterparts. Degradation in a PBS+collagenase XI environment showed that mineralization extended total time to degradation. It was also shown that the formation of the Ca-P crystals had no negative effects on in vitro cell studies. To measure cellular response, a live/dead assay was conducted to confirm cell viability after 24 hours.
In conclusion, improvements were made to mechanical strength without compromising in vitro cell-scaffold response. While it remains unknown whether the increase in strength is adequate for use as a bone scaffold, future work should focus on gathering necessary information to study OVA scaffolds in animal models for eventual consideration as a bone graft substitute material.
âMaster of Science
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Katayama, Hirokazu. "Studies on ovalbumin and β-lactoglobulin aggregates induced by urea and their functionalities." Kyoto University, 2004. http://hdl.handle.net/2433/145424.
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Kyoto University (京都大学)0048
新制・課程博士
博士(農学)
甲第11111号
農博第1441号
新制||農||898(附属図書館)
学位論文||H16||N3961(農学部図書室)
22661
UT51-2004-L908
京都大学大学院農学研究科農学専攻
(主査)教授 松村 康生, 教授 内海 成, 教授 北畠 直文
学位規則第4条第1項該当
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Cabral, Priscilla Carvalho. "Desenvolvimento de modelo experimental murino para o estudo da imunobiologia do melanoma." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/42/42133/tde-16112016-093857/.
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O câncer compreende uma doença multifatorial responsável por altíssimos indíces de mortalidade globalmente. Embora atualmente tenhamos resultados positivos em relação ao tratamento do câncer principalmente voltados à imunoterapia, dados alarmantes ainda são encontrados. Assim, desenvolvemos linhagens tumorais geneticamente modificadas para expressarem ovalbumina (mOVA ou cOVA) e luciferase, a fim de estudarmos as interações do sistema imune com o tumor. Em nossos resultados, a presença da ovalbumina indicou: Alteração no perfil de crescimento tumoral em animais previamente imunizados com OVA e posteriormente desafiados com o tumor, ativação de células TCD8+ citóxicas anti-OVA além de demonstrar também a capacidade imunogênica das linhagens tumorais quando estas são administradas nos animais em estado necrótico. Ao todo, nosso modelo demonstrou que estratégias de vacinação anti-tumorais possuem a capacidade de ativação do sistema imune para na otimização do reconhecimento e resposta anti-tumoral.Cancer is characterized as a multifactorial disease responsible for many deaths globally. Although nowadays we can find positive perspectives regarding cancers treatment, it is still very common to notice some alarming data. Therefore, our group developed some genetically modified tumoral lineages expressing ovalbumin (mOVA or cOVA) together with luciferase, in order to elucidate the relationship between tumor and the immune system. In our results, the presence of ovalbumin demonstrated: Changes in tumoral growth when animals were previously immunized with OVA and then challenged with our tumoral lineages; TCD8+ lymphocytes anti-OVA activation thus ovalbumin immunogenic potential when lineages were exposed to necrotic death followed by in vivo administration. In summary, our model showed that anti-tumoral vaccinations are indeed capable of promoting immune systems activation and consequently, improving the anti-tumor immunity.
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Olivo, Clarice Rosa. "Efeito do condicionamento físico aeróbico de moderada intensidade na inflamação pulmonar alérgica crônica e na hiperresponsividade brônquica à metacolina em cobaias sensibilizadas." Universidade de São Paulo, 2009. http://www.teses.usp.br/teses/disponiveis/5/5163/tde-06112009-133329/.
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O treinamento físico (TF) melhora a resposta imune de indivíduos saudáveis e traz benefícios para o paciente asmático, mas seu papel na resposta alérgica é desconhecido. Objetivo: Avaliar o papel do TF de moderada intensidade na inflamação pulmonar alérgica crônica. Métodos: 54 cobaias, divididas em 4 grupos: grupo controle (C) (não sensibilizados e não treinados), grupo OVA (sensibilizados à ovalbumina (OVA) e não treinados), grupo treinamento físico (TF) (não sensibilizados e submetidos a um TF), e grupo OVA+TF (sensibilizados à OVA e submetidos a um TF). A sensibilização à OVA teve duração de 8 semanas e o programa de TF de 6 semanas iniciando 15 dias após o início da sensibilização. Cada grupo foi dividido em 2 subgrupos. No primeiro foi avaliada a inflamação pulmonar e os níveis de óxido nítrico exalado (NOex) e no segundo, a hiperresponsividade brônquica à metacolina (Mch). Resultados: A sensibilização à OVA induziu a um aumento da densidade de eosinófilos e linfócitos, expressão de IL(interleucina)-4 e IL-13 e na espessura do músculo liso na via aerea assim como espessura do epitélio comparado aos animais não-sensibilizados (p<0,05). Os animais do grupo OVA+TF apresentaram uma redução da densidade de eosinófilos, linfócitos, IL-4 e IL-13 comparado com o grupo OVA (p<0,05). Nem a sensibilização crônica a OVA ou TF influenciaram a expressão das citocinas Th1 (IL-2 e IFN-) ou a expressão das citocinas regulatórias (IL-10 e IL-1-ra) e nos níveis de NOex. Os grupos que realizaram TF tiveram aumento na espessura do epitélio quando comparados com grupos não-treinados embora não há diferença entre os grupos na avaliação da hiperresponsividade brônquica. Conclusão: Nossos resultados sugerem que o TF reduz a inflamação alérgica sem modificar a hiperresponsividade brônquica e o remodelamento das vias aéreasBackground: Aerobic training (TF) has a positive effects on health subjects and bring benefits on the immune system of asthmatic patients. However, its role on allergic immune response remains poorly understood. Objective: To evaluate the effects of TF in chronic allergic inflammation. Methods: Fiftyfour animals, divided in 4 groups: non-trained and non-sensitized (C), nonsensitized and aerobic exercise (TF), ovalbumin sensitized and non-trained (OVA), and sensitized and aerobic exercise (OVA+TF). OVA or saline sensitization was performed during 8 weeks. TF was performed in a treadmill during 6 weeks beginning in the 3rd week of sensitization. Each group were divided in two groups. In the first one, it was evaluated airway inflammation and levels of exhaleted oxide nitric (NOex), on the second, airway hyperresponsiveness to a methacholine (Mch). Results: OVA sensitization induced an increase in the eosinophils and lymphocytes counting, expression of IL-4 and IL-13 and the amount of airway smooth muscle and epithelium thickness compared to non-sensitized animals (p<0.05). Sensitized animals submitted to TF presented a reduction in the eosinophil and lymphocyte counting, expression of IL-4 and IL-13 compared with OVA group (p<0.05) but not OVA-induced changes in airway remodeling (p>0.05). Neither OVA nor TF induced any difference in the expression of Th1 (IL-2 and IFN-) and regulatory cytokine (IL-10 and IL1-ra) and the levels of NOex. Trained groups presented an increase in epithelium thickness as compared to the nontrained groups however we did not find difference between groups on hyperresponsiveness avaliation. Conclusion: Our results suggest that TF reduces allergic airway inflammation without changes in bronchial hyperresponsiveness and airway remodeling
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Medeiros, Karina Carla de Paula. "Efeito imunomodulador do Kanferol glicolisado em modelo de asma alérgica experimental." Universidade Federal da Paraíba, 2009. http://tede.biblioteca.ufpb.br:8080/handle/tede/6846.
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Previous issue date: 2009-04-01Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES
Asthma is a chronic inflammatory disease characterized by cell migration and bronchial hyperreactivity (HRB). One strategy to treat allergic diseases is the development of new drugs with good efficacy and few side effects. Flavonoids are plant compounds known for its antioxidant, antiallergic and anti-inflammatory activities. Based on this premise, the study aimed to select flavonoids by the immunopharmacological screening and to evaluate prophylactic and therapeutic treatments in the experimental asthma model. For this purpose the flavonoids tested were myricetin, isoramnetin and glycosylated kampherol known as 3-O-[β-D-glycopiranosil-(1→6)-α-L-rhamnopyranosyl]-7-O-α-L-rhamnopyranosyl-kamferol (GRRK). The experimental model was BALB/c mice sensitized and challenged with ovalbumin (OVA) and the treatments were before (prophylactic) or after (therapeutic) to establishment of the experimental asthma model. The three flavonoids inhibited the animal death during the anaphylactic chock reaction induced by OVA and the inflammatory cell migration to the lung; however only the GRRK was used in the following experiments because of its best yield in the purification process. Both prophylactic and therapeutic treatments with GRRK (30 mg/kg) decreased significantly the total number of inflammatory cells (P< 0.05), eosinophils cells (P< 0.05), IL-5 (P< 0.05) and IL-13 (P< 0.05) production from bronchoalveolar lavage (BAL), IgE OVA-specific title (P< 0.01) in the sera, the bronchial hyperreactivity (HRB) (P< 0.05) induced by methacholine and mucus production (P< 0.001) by the globbet cells from the lung as compared with non-treated GRRK animals. The results obtained in the different treatments with GRRK were statistically comparable with that observed in the OVA-sensitized group treated with dexametasone. Moreover, treatment with GRRK was able to reduce the number of CD4+ T cells (p <0.001), B cells (p <0.001), expression of major complex of compatibility II class (MHC II class) and CD40 molecules in the antigen presenting cells (CD11b+ CD11c+) from BAL. Although the GRRK induced a suppressive effect in the Th2 immune response it was not able to exacerbate the Th1 immune response by IFN- production. The production of this cytokine was not modified when compared with non treated animals. The GRRK treatment also was not able to modify the regulatory immune response because there was no significant change in the production of TGF- and the number of TCD4+Foxp3+, a marker of LT regulatory (TReg). These results demonstrated that GRRK treatment before (prophylactic) or after (therapeutic) the establishment of allergic asthma, restored the morpho-functional changes in the airways by Th2-dependent immunossupression, although independent of Th1 and TReg.
A asma é uma doença inflamatória crônica caracterizada por migração celular para os pulmões e hiperreatividade brônquica (HRB). Uma estratégia para o tratamento de doenças alérgicas é o desenvolvimento de novos medicamentos com boa eficácia e poucos efeitos colaterais. Flavonóides são compostos derivados de plantas conhecidos por suas atividades antioxidante, antialérgica e antiinflamatória. Partindo desta premissa, o trabalho teve como objetivo selecionar flavonóides a partir de análises imunofarmacológicas e avaliar os tratamentos profiláticos ou terapêuticos no modelo de asma alérgica experimental. Para tal, os flavonóides testados foram: myricetina, isoramnetina e kanferol glicosilado conhecido por 3-O-[β-D-glycopiranosil-(1→6)-α-L-ramnopiranosil]-7-O-α-L-ramnopiranosil-kanferol (GRRK). O modelo experimental foi realizado em camundongos BALB/c sensibilizados e desafiados com ovalbumina (OVA) e os tratamentos foram antes (profilático) ou após (terapêutico) estabelecer o modelo de asma alérgica experimental. Os três flavonóides inibiram a morte dos animais durante o choque anafilático induzido por OVA e a migração de células da inflamação para os pulmões, entretanto, apenas o GRRK foi usado nos demais experimentos, devido ao seu melhor rendimento no processo de purificação. Os tratamentos profilático e terapêutico com GRRK (30 mg/kg) diminuíram, de forma significativa, o número total de células inflamatórias (P< 0,05), de eosinófilos (P< 0,05) a produção de IL-5 (P< 0,05) e IL-13 (P< 0,05) no lavado broncoalveolar (LBA), o título de IgE-OVA-específica (P< 0,01) no soro, a hiperreatividade brônquica (HRB) (P< 0,05) induzida com metacolina e a produção de muco (P< 0,001) pelas células caliciformes de pulmão quando comparados com os animais não tratados com GRRK e sensibilizado com OVA. Os resultados obtidos nos diferentes tratamentos com GRRK foram comparáveis estatisticamente aos efeitos observados no grupo de animais tratados com a droga padrão dexametasona. Além disso, o tratamento com GRRK foi capaz de diminuir: o número de linfócitos T CD4+ (P< 0,001) e de linfócitos B (P< 0,001), a expressão de moléculas de classe II do complexo principal de histocompatibilidade (MHC II) e da molécula CD40 em células apresentadoras de antígeno (CD11b+CD11c+) do LBA. Embora o tratamento com GRRK tenha induzido um efeito supressor na resposta imune de perfil Th2, não foi capaz de exacerbar a resposta imune Th1 com a produção de IFN- A produção desta citocina se manteve inalterada quando comparada ao grupo de animais não tratados. O tratamento também não foi capaz de alterar a reposta imune reguladora, pois não houve mudança significativa na produção de TGF- e no número de células TCD4+Foxp3+, marcador de LT regulador (LTReg). Esses resultados demonstram que o tratamento com GRRK antes (profilático) ou depois (terapêutico) de estabelecer a asma alérgica experimental, restabeleceu as alterações morfofuncionais das vias aéreas, por um mecanismo modulador das células Th2, embora independente de Th1 e de Treg
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Köchling, Hanna Kathrin [Verfasser]. "Etablierung des Ovalbumin-induzierten Mausmodells der atopischen Dermatitis zur Beurteilung pharmakologischer Wirkstoffe / Hanna Kathrin Köchling." Hannover : Bibliothek der Tierärztlichen Hochschule Hannover, 2015. http://d-nb.info/1080815384/34.
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Haener, Edgar. "Microfluidic segregation of capsules." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/microfluidic-segregation-of-capsules(a7e001f1-536c-475d-83d5-82aaa4098f5b).html.
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This thesis investigates the transport and sorting of capsules (elastic membranes enclosing a liquid core) using viscous flow in complex vessel geometries. Of particular interest is passive sorting by deformability using only the fluid-structure interaction between the capsule, the viscous fluid and the geometry of the vessel. Millimetric alginate-ovalbumin capsules in the regime of negligible fluid inertia are used in this work. In order to characterise the elastic properties of the capsules, a novel numerical finite element model of the compression of a thick-shelled capsule between parallel plates is implemented. The constitutive model of the capsule membranes was determined by comparison to experimental data: a Yeoh constitutive model with the ratio of constants $C_1 = 1$, $C_2 = 0$ and $C_3 = 10$ describes the capsules used. Three geometries are investigated in this work. (i) A T-Junction bifurcation. Capsule deformation in the T-Junction bifurcation is characterised by the maximal length of the capsule $L_{max}$ and depends on the ratio of viscous to elastic forces, the capillary number $Ca$. The maximal length, $L_{max}$, is especially sensitive at distinguishing soft capsules by their deformability. The sensitivity of $L_{max}$ to capsule compliance and the large deformations that can be achieved makes the T-junction a promising geometry in which to measure elastic properties of the capsules. The rate of relaxation of the capsules after the bifurcation is independent of their deformation. (ii) A half-cylinder obstacle in a channel followed by a sudden expansion. We show that the half-cylinder obstacle causes capsule trajectories to vary depending on deformability. Capsules with a factor of three difference in deformability can be separated. A practical feature of the system is its relative insensitivity to the initial lateral position of the capsules in the channel. However, while the results are reproducible across different capsules, the variations in final position amount to 10 \% at fixed parameters. As these experiments were conducted with the same capsule under identical flow conditions, this is likely to represent the best case scenario. (iii) We adapt the pinched flow fractionation (PFF) geometry to the sorting of capsules. We show that the standard PFF device cannot be used to sort capsules. However, a novel mode of operation, termed the ``T-Junction'' mode, shows great promise for the sorting of capsules. The PFF device in the T-Junction mode separates capsules with a factor of 1.5 difference in deformability. This is twice as sensitive as the half-cylinder device, although larger variability was observed in the PFF device.
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Goodacre, J. A. "A study of the frequency of mouse cells which present alloantigens and ovalbumin to T lymphocytes." Thesis, University of Newcastle Upon Tyne, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233305.
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Janke, Marko [Verfasser]. "Die Rolle von antigenspezifischen Helfer-T-Zellen im murinen Krankheitsmodell der Ovalbumin-induzierten Arthritis / Marko Janke." Berlin : Freie Universität Berlin, 2009. http://d-nb.info/1023666286/34.
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Chen, Liying. "Processing and presentation of exogenous antigen by dendritic cells /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-890-8/.
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Dong, Caroline. "Effect of diesel exhaust particles on allergic reactions and airway responsiveness in ovalbumin-sensitized brown Norway rats." Morgantown, W. Va. : [West Virginia University Libraries], 2007. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=5046.
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Thesis (Ph. D.)--West Virginia University, 2007.Title from document title page. Document formatted into pages; contains xi, 118 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 98-118).
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Kasahara, David Itiro. "Beta2-agonista como imunomodulador da resposta inflamatória pulmonar crônica induzida em camundongos sensibilizados com ovoalbumina." Universidade de São Paulo, 2005. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-09102014-091043/.
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Estudamos o efeito do tratamento com salbutamol em dois regimes: diário (DS) e administrado a intervalos de 96 horas (IS) em camundongos balb/c sensibilizados com injeções intraperitoneais de uma solução de ovoalbumina (OVA) adsorvida em hidróxido de alumínio, e desafiada com inalações de ovoalbumina a 1%. O grupo controle SAL recebeu injeções i.p. de salina e desafios inalatórios de sallina. A partir do 34o dia, os animais OVA foram tratados com salbutamol via inalatória 10 mg/ml durante 15 minutos nos dois regimes descritos. Os animais foram sacrificados no 60o dia, que corresponde a 48 horas após o último desafio antigênico. Após os camundongos serem anestesiados com pentobarbital sódico via i.p., eles foram traqueostomizados e entubados e sacrificados com secção da Aorta abdominal. Então, procedeu-se com a coleta do lavado broncoalveolar para a quantificação de leucócitos. Coletamos os tecidos pulmonares para a avaliação do processo inflamatório por quantificação de células linfomononucleares (LMN) e eosinófilos EPO+, essa última com marcação citoquímica. Além disso, estudamos a influência do tratamento adrenérgico sobre o IgE anafilático. O modelo de inflamação (grupo OVA) produziu significativo aumento do número de células totais, de eosinófilos e de neutrófilos observados na avaliação de lavado broncoalveolar. Além disso, houve nesse grupo processo inflamatório na parede de vias aéreas, caracterizada por um infiltrado linfomononuclear e com presença de eosinófilos. O nosso processo de indução de inflamação também recrutou eosinófilos para o septo alveolar. O tratamento com salbutamol diário produziu uma queda significativa do processo inflamatório no BAL, principalmente de neutrófilos e eosinófilos, enquanto que o tratamento intermitente produziu redução significativa apenas de neutrófilos. O tratamento com salbutamol a cada 96 horas (IS) promoveu uma queda significativa de células LMN quantificadas no septo alveolar, mas não atingindo valores do grupo salina (NS). Ambos os tratamentos com salbutamol produziu redução significativa de células EPO+ no parênquima pulmonar (P < 0,05). Apesar das alterações no processo celular, o salbutamol não influenciou na expressão de anticorpos IgE anafiláticos a OVA. Assim, podemos concluir que o salbutamol apresenta atividade imunomoduladora, observada por redução de eosinófilos no BAL e no parênquima pulmonar, apesar de não atingir valores semelhantes aos animais do grupo salinaWe studied the effects of salbutamol treatment in two regimen: diary (DS) and at interval of 96 hours (IS) in ovalbumin sensitized (OVA) balb/c mice. The control group (NS) received i.p. injections and aerosol challenge with normal saline. Starting at day 34 the OVA animals were treated with 10mg/ml salbutamol by inhalation during 15 minutes per day in both regimen: DS and IS. The mice were sacrificed at day 60 that corresponded the fourthly eight hours after last OVA and/or salbutamol exposure. At experimental day, mice were anesthetized with i.p. injection of sodium pentobarbital, tracheostomized, entubed and the abdominal aorta sectioned. We followed with collecting of bronchoalveolar lavage (BAL) and lungs to histopathology studies. In the BAL, total cells and differential leukocytes were quantified, while in the lung sections, the EPO+ and LMN in airways wall and parenchyma septa were evaluated. Also, we sampled the blood to evaluate the effects of salbutamol on anaphylactic IgE antibodies expression. The inflammatory model (OVA animals) produced a significant increase of BAL total cells, BAL eosinophils and neutrophils, and LMN cells and EPO+ eosinophils in the airways and in the parenchyma. Diary salbutamol treatment decrease significantly BAL eosinophils and neutrophils, while the IS group showed a diminution of BAL neutrophils and LMN cells in the alveolar septum. Both salbutamol treatments produced significant decline of EPO+ cells in the lung parenchyma. Despite the changes in the cellular patterns, the salbutamol did not affect the IgE antibodies expression. So, we can concluded that salbutamol present an immunomodulatory activity observed by reduction of eosinophils in the BAL and lung parenchyma, but did not achieve the values of saline control group
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Shen, Xiaodong. "Characterization of sugars and glycans from ovalbumin using a combination of derivatization, high-performance liquid chromatography and mass spectrometry." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ35084.pdf.
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Wang, Junji. "Therapeutic vaccines based on myobacterium vaccae for the treatment of an IgE response to ovalbumin in BALB/c mice." Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285207.
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Brummer, Mieke. "THE INFLUENCE OF SELENIUM STATUS ON IMMUNE FUNCTION AND ANTIOXIDANT STATUS IN THE HORSE." UKnowledge, 2012. http://uknowledge.uky.edu/animalsci_etds/7.
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Selenium (Se) has received a lot of attention for its antioxidant and immune modulating properties. Yet, comparably few studies have focused on the horse. Therefore the objectives of this research were to evaluate the influences of Se status on immune function and antioxidant defense in horses. Twenty eight horses were allocated to one of 4 dietary Se treatments: low (LS), adequate (AS), high organic (SP) and high inorganic (SS). First, horses assigned to LS, SP and SS were depleted of Se and received a low Se diet (0.07 ppm Se) for 35 wk, while AS received an adequate Se diet (0.14 ppm Se). During week 28 to 35 immune function was evaluated using a vaccine challenge with keyhole limpet hemocyanin (KLH) and equine influenza as antigens. Then, a 29 wk repletion phase followed. The LS and AS received the same diets described above while SP received an organic Se supplemented diet (0.3 ppm; Sel-Plex, Alltech, Nicholasville, KY) and SS an inorganic Se supplemented diet (0.3 ppm; sodium selenite). Immune function was assessed using a vaccine challenge with ovalbumin (OVA) and equine influenza as antigens during week 22 to 29. Samples collected throughout the depletion and repletion phases were used to assess change in Se status, antioxidant status and oxidative stress. Finally, a mild exercise test served to assess exercise induced oxidative stress. The experimental model responded as hypothesized, evaluated by blood Se and glutathione peroxidase (GPx) activity. Upon vaccination with KLH, antibody response was faster in AS than LS. Antigen specific mRNA expression of T-bet was also higher for AS than LS. Following OVA vaccination humoral and cell-mediated vaccination responses were similar across treatments. However, non-specific stimulation of peripheral blood mononuclear cells indicated suppressed mRNA expression of selected cytokines for LS compared to AS, SP and SS. Antioxidant capacity and oxidative stress were unaffected by change in Se status. A difference in GPx response post exercise was also noted between SP and SS. Low Se status impaired some measures of immune function. Supplementation at 0.3 ppm may benefit horses as indicated by higher GPx activity in idle and exercised horses.
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RONKAINEN-MATSUNO, NIINA JOHANNA. "BEAD-BASED IMMUNOASSAYS WITH ELECTROCHEMICAL DETECTION." University of Cincinnati / OhioLINK, 2003. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1069082511.
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Lutje, Vittoria. "Proliferative and antibody responses induced by pokeweed mitogen, sheep erythrocytes and ovalbumin in bovine leukocyte populations and the cellular interactions involved." Thesis, Brunel University, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.280691.
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Tang, Ching-chia, and 湯景家. "Studies on Membrane-Perturbing Activities of Glycated Ovalbumin." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/61448684717374399650.
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碩士國立中山大學
生物醫學研究所
104
The aim of this study was to investigate the functional and structural properties of ovalbumin (OVA) with glycated carboxyl groups. Mannosylated OVA (Man-OVA) was prepared by modification of its carboxyl groups with p-aminophenyl-α-D- mannopyranoside. MALDI-TOF mass and RP-HPLC analyses revealed that, in comparison with OVA, Man-OVA showed an increase in molecular weight and hydrophilicity. Glycation of carboxyl groups caused a notable change in the secondary structure of OVA as evidenced by CD spectra measurement. In contrast to OVA, Man-OVA showed dose-dependently membrane-damaging activity and fusogenicity. The magnitude of membrane-damaging activity increased with increasing number of modified carboxyl groups. Meanwhile, membrane-damaging activity and fusogenicity of Man-OVA were affected by the lipid compositions of vesicles. Iodide quenching revealed that the microenvironment of Trp residues was abundant in positively charged character after glycation of carboxyl groups. Fluorescence spectra measurement and acrylamide quenching studies showed that the spatial position of Trp residues in Man-OVA became exposed compared with that in OVA. Furthermore, upon binding with lipid vesicles, the susceptibility of Trp residues for acrylamide reduced in Man-OVA and OVA. Although OVA and Man-OVA had similar binding affinity for lipid vesicles, Man-OVA and OVA were absorbed on lipid bilayers in different manners as revealed by color transformation of phospholipid/polydiacetylene membrane assay. Chemical modifications of Lys, Arg and Trp residues showed that Lys and Arg residues rather than Trp residues played a crucial role in membrane-damaging activity of Man-OVA. Taken together, our data indicate that glycation of the carboxyl groups causes a change in gross conformation and an increases in positively charged character of OVA, leading to generate functional OVA with membrane-perturbing activities on the lipid-water interface.
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Wu, Mei-Yao, and 吳美瑤. "Immunosuppressive effects of fisetin in ovalbumin-induced asthma." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/81746621525480172591.
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博士國立陽明大學
傳統醫藥研究所
100
Asthma is a chronic inflammatory disease with the characteristics of airway inflammation, airway hyperresponsiveness, and airway mucus production. Activation of NF-B is highly associated with the pathogenesis of asthma, and previous studies reveal that NF-κB is a promising molecular target in the treatment of asthma. Natural products present in food or Chinese medicinal herbs may have potential on inhibition of NF-κB activity and attenuation of allergic asthma. In this study, I first screened for compounds isolated from food and traditional Chinese medicinal herbs on the suppressive effects of NF-κB activity. Fisetin, a flavonoid compound commonly present in fruits and vegetables, can exert anti-inflammatory activities via inhibition of NF-κB-signaling pathway. I further evaluated the anti-asthma activity of fisetin and its possible molecular mechanisms. Fisetin attenuated lung inflammation, goblet cell hyperplasia and airway hyperresponsiveness in ovalbumin-induced asthma, and decreased eosinophils and lymphocytes in bronchoalveolar lavage fluid. Fisetin treatment reduced expressions of the key initiators of allergic airway inflammation (eotaxin-1 and thymic stromal lymphopoietin), Th2-associated cytokines (IL-4, IL-5, and IL-13) in lungs, and Th2-predominant transcription factor gata-3 and cytokines in thoracic lymph node cells and splenocytes. Notably, fisetin treatment impaired NF-κB activation in ovalbumin-stimulated lung tissues and TNF-α-stimulated bronchial epithelial cells. Collectively, the data demonstrate the beneficial effects of fisetin in the amelioration of asthmatic phenotypes. The anti-asthma activity of fisetin is associated with reduction of Th2 responses as well as suppression of NF-κB activity and its downstream cytokines and chemokines. I also demonstrated in this study that puerarin suppressed PHA-stimulated IL-2 expression and inhibited the ERK1/2-c-Fos signaling pathway induced by PHA-stimulation in Jurkat cells. Puerarin may have potential in immunosuppression and in asthma treatment.
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Chan, Cheng Chi, and 詹政己. "Fetal exposure ovalbumin resulting in murine hyper-immune responsiveness." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/64n699.
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博士長庚大學
生物醫學研究所
104
The prevalence of allergy and asthma is gradually increasing in childhood over the past decades. Asthma, a chronic airway inflammation, is driven by T helper 2 (Th2)-type immunity and characterized by airway hyperresponsiveness, eosinophilic infiltration, and elevated IgE production. Various novel strategies for managing asthma have been explored, such as allergen-specific immunotherapy. A principal goal of most immunotherapeutic approaches is active and long-term allergen-specific tolerance. Environmental factors are well accepted as an important risk to interact with genetic susceptibility of allergic patients and promote the development of allergic diseases. Gene-environment interactions during prenatal or postnatal period alter immune programming and affect the fate of infant’s allergic disease. However, the relationship between prenatal allergen exposure and allergic disease development is still a controversial issue. In this study, we want to investigate whether prenatal allergen exposure induces immune tolerance or sensitization to allergen. The peritoneal cavity of each fetus was directly injected with different doses of adjuvant-free ovalbumin (OVA) on day 14 of gestation. In utero OVA-injected adult mice challenged by inhaling OVA aerosols manifested significant induction of airway hyperresponsiveness, lung eosinophilia, OVA-specific antibodies, and Th2 cytokines. These adult mice also developed serious anaphylactic reactions following intraperitoneal injection of OVA. In neonatal period, in utero OVA-injected mice already had potent OVA-specific humoral and cell-mediated immunity. Cytokine expression pattern in the lungs of in utero OVA-injected neonates evidently favored Th2-biased immune responses. These results suggested that prenatal allergen exposure modified immune profile and facilitate allergic asthma development. To further dissect the mechanisms of hyper-immune responsiveness, we focused possible candidate molecules on costimulatory molecules and Notch signaling. Although increased inducible costimulator (ICOS) levels were detected in spleen CD4+ T cells of in utero OVA-injected neonates, ICOS blockade did not obviously suppress Th2 cytokine production of in utero OVA-injected neonates. Furthermore, splenocytes of in utero OVA-injected neonates expressed higher levels of Jagged1 and Jagged2 RNA. Inhibition of Notch signaling by γ-secretase inhibitor significantly reduced Th2 cytokine production and T-cell proliferative responses to OVA in vitro. Notch signaling pathway may be involved in regulating the Th2 cell-driven hyper-immune responsiveness of in utero allergen exposure. Consequently, intervention of allergen exposure or Notch signaling during pregnancy may be beneficial for modulating the development of allergic asthma.
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Huang, Chu-Ping, and 黃楚評. "Cloning and Fuctional Assessment of the Chicken Ovalbumin Promoter Sequences." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/87613227551870692303.
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碩士國立臺灣大學
畜產學研究所
93
A modern genetically selected White Leghorn hen lays up to 330 eggs per year and the total proteins contained within each egg has been averaged around 6.6 grams, including 3.5 grams of the egg-white proteins and 3.1grams of the yolk proteins. Of 3.5 grams of the egg-white proteins, 2 grams of them have been identified as the ovalbumin. The fact that egg-white proteins are primarily synthesized and secreted from the tubular gland cells lined on the lumen of the magnum of the oviduct, indicating that these tubular gland cells found in the magnum of laying chicken possess a great potency of serving as the bioreactor for production of transgenic proteins. Attempts of the present studies were made to clone the promoter sequences of ovalbumin gene and to evaluate the potency of using the cloned promoter for construction of the transgene(s). To meet with the described purpose, genomic DNAs extracted from blood cells of the laying chicken hens were subjected to cloning of the ovalbumin promoter sequences by the strategy of PCR-TA cloning techniques that have resulted in a fragment of the genomic DNA lengthen in 3.1 kb was successfully amplified and ligated into the pGlow-TOPO vector. Of the 3.1 kb fragment after sequencing analysis, a 1.3 kb in length of the 5’ end sequences was confirmed to contain the promoter region and the rest fragment lengthen in 1.8 kb of the 3’ end was characterized as the sequences encoding the leading protein. The efficacy of the cloned ovalbumin promoter was further evaluated using the green fluorescence protein (GFP) as a reporter. Of these studies, a transgene named pMAR-OV-GFP was constructed allowing the full length of GFP cDNA sequences driven by ovalbumin promoter and the OV-GFP sequences were equipped with a matrix attachment region (MAR) that will ensure the transgene successfully escaped from damage by those topoisomerases. The expression of transgene of pMAR-OV-GFP was evidenced from the observation of GFP fluorescence detected by fluorescent microscope around 24-48 post the transgene had been transfected into the primary cultured tubular gland cells of oviduct magnum from the laying hen. These results indicate that the cloned ovalbumin promoter does possess potency to drive the GFP cDNA while the efficiency is still far beyond satisfy.
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Kinder, Jeremy M. "Disruption of esophageal tissue hinders oral tolerance induction to ovalbumin." 2012. http://liblink.bsu.edu/uhtbin/catkey/1670054.
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Previous data in our lab demonstrated an inability to induce oral tolerance when using a
feeding needle gavage for 14 days. Given that the upper gastrointestinal (GI) tract is the site of
antigen introduction, and the interplay between immune cells of the mucosal tissues, we
questioned if inflammation in this tissue, induced by physical trauma, would affect oral tolerance
induction. We performed studies on Balb/c mice using a needle gavage or syringe feeding
method followed by doses of the immunogenic protein ovalbumin (OVA) to induce tolerance.
Immunohistochemistry was used to assess inflammation in esophageal tissues and to correlate
with an ability or inability to induce tolerance. Non-cellular alterations within the tissue were
also assessed using a pathology grading score. Although fluctuations in cell populations were
observed in both the syringe and gavage treated mice, the needle gavage caused significant noncellular
damage to esophageal mucosal tissue, which is the most likely cause of failed tolerance
induction to the OVA.Department of Biology
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Tsai, Shu-Mei, and 蔡淑玫. "Quercetin-3,5,7,3',4'-O-pentamethylether inhibits ovalbumin-induced airway hyperresponsiveness." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/38798258337298107157.
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碩士臺北醫學大學
藥理學研究所
94
Quercetin-3,5,7,3',4'-O-pentamethylether (QPME) inhibited activities of PDE1~4, with IC50 values < 10 M. The PDE4H/PDE4L ratio of QPME is about 11, equal to that of AWD 12-281 which is in clinical trial phase II. QPME whether possesses anti-asthmatic effect is the aim of this investigation. In vivo, female BALB/c mice were sensitized by an intraperitoneal injection of ovalbumin (OVA), then challenged via the airway by ultrasonic nebulization of 1% OVA two periods (secondary challenge). After secondary challenge, the airway hyperresponsiveness (AHR) was measured in unrestrained animals, nebulized with methacholine (MCh, 6.25~50 mg/ml), by barometric plethysmography using a whole-body plethysmograph. In the present results, QPME (10~100 mol/kg, i.p.) dose-dependently attenuated the enhanced pause (Penh) value induced by MCh (25~50 mg/ml). Furthermore, QPME (100 mol/kg, i.p.) also significantly inhibited MCh (25 mg/ml)-induced Penh value. QPME (10-100 mol/kg, i.p.) also significantly inhibited total inflammatory cells, macrophages, neutrophils , lymphocytes, and eosinophils in BALF after determination of Penh values. It also significantly attenuated the release of IL-2, IL-4, IL-5, IFN-γ, and TNF-α, with some exceptions that QPME at the least dose did not suppress releases of total inflammatory cells, macrophages, IL-5, and IFN-γ. In vitro, QPME (30~100 M) significantly relaxed baseline tension and inhibited cumulative OVA (10~100 μg/ml)-induced contractions in isolated sensitized guinea pig trachealis. According to the Lineweaver-Burk analysis, QPME (3~30 M) competitively inhibited PDE1, PDE2, PDE3, and PDE4 activities. The Ki values were 0.89, 1.07, 0.53, and 0.52 M which did not differ each other. Owing to QPME did not selectively inhibited PDE1-4, and resulted increase of intracellular cAMP, it may possess anti-inflammatory and anti-asthmatic effects.
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Cheng, PeiYun, and 鄭佩芸. "Lovastatin reduces lung inflammatory responses in ovalbumin-sensitized asthmatic mice." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/69041097419508011762.
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碩士長庚大學
生物醫學研究所
98
Allergic asthma disease is characterized by Th2 cells and eosinophils infiltration in lung. Th2 cells were activated by antigen leading to secret cytokines. Eosinophil infiltration in airway is also associated with Th2 responses, which is important in developing airway inflammation and airway hyperresponsiveness (AHR) in asthma. Regulating the inflammation responses has been the target for ameliorating asthma. Statins are 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors. Although they have been commonly prescribed for cardiovascular diseases, the anti-inflammatory role of lovastatin in allergic asthma had been reported. In this study, we tested the effects of lovastatin on reducing inflammatory responses in ovalbumin-sensitized mice. Mice received low (10 mg/kg) or high dose (40 mg/kg) of lovastatin by intragastric feeding for seven days (7d) or fourteen days (14d) before OVA challenge. The 7d treatment demonstrated that high dose lovastatin treatment group had significantly lower AHR and eosinophil infiltration than sensitized control group. Furthermore, significant low level of AHR and eosinophil infiltration was observed in 14d of low dose and high dose lovastatin than sensitized control group. There was lower eotaxin-1 (CCL11) concentration in bronchoalveolar lavage fluid compared to sensitized control group. In addition, the chemokines (eotaxin-1 and eotaxin-2), VCAM-1, and IL-6 levels of lovastatin treatment groups in RNA expression levels were lower than sensitized groups of 14d lovastatin administration. Significantly lower Th2 cytokines levels in OVA-specific splenocyte culture were detected in lovastatin treatment groups than that of sensitized group. However, no significant effect was detected for serum OVA-specific IgG1 and IgG2a concentrations of both 7d and 14d lovastatin treatment groups. In addition, OVA-specific IgE level was significantly decreased by lowest lovastatin concentration administration. These data suggested that lovastatin attenuated eotaxin expression levels in the lung that leading to reduced asthmatic symptoms.
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Zhang, Chun Min, and 張淳閔. "Oligochitosan modification on ovalbumin and zein for mammalian cell transfection." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/y8k4b4.
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Tsai, Ho-Ching, and 蔡和澋. "Establishment of oviduct epithelial cells culture system for ovalbumin promoter evaluation." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/03240143174648341830.
Full textAbstract:
碩士國立臺灣大學
動物科學技術學研究所
105
Research models of transgenic avian have been widely applied for generating exogenous proteins in eggs because of their relatively short generation interval and stable protein productions. Albumin constitutes more than half of the protein in eggs, and ovalbumin (OVA) is the primary component. According to the composition of egg white and the tissue-specific expression of ovalbumin, most of the published exogenous proteins are driven by ovalbumin promoter and detected in egg white. However, in vitro functional studies on avian oviductal epithelial cells (OECs) in magnum segments, where egg white are synthesized and secreted, are needed. In this study, we established and the primary culture system for OECs in order to study the regulation of ovalbumin promoter activity. The magnum segments of oviducts from sexually-mature female Japanese quails were dissected for OECs collection. After scraping epithelial cells from the lumen surface, the obtained cells were then underwent enzymatic dissociation. For improving cell survival rate and proliferation, different concentrations of digestion enzymes and culture dish coating materials were examined. Results showed that digestion solution containing collagenase type IV (275 units/ mL) and 0.02% trypsin worked the best for OECs isolation. The 0.1% gelatin solution is required for coating culture plate to enhance OECs attachment. We examined protein expression of OVA and estrogen receptor 1 (ESR1) on cultured OECs by immunofluorescent staining and Western blotting. For studying OVA promoter activity regulation, the OECs were treated with several concentrations of estrogen (E2), progesterone (P4) and Dexamethasone to examine the effects of different hormones on the expression of OVA. We found that OVA mRNA expression was significantly increased when treated with 2.5×10-7 M E2, 1×10-7 M P4 and 1×10-7 M Dexamethasone. Meanwhile, we cloned a 2.8-kb OVA promoter from chicken genomic DNA and constructed with the luciferase reporter vector (pGL3). We then transfected the pGL3-OVA construction into both OECs and 293T cell line and treated with E2, P4 and Dexamethasone to measure promoter activity using a reporter gene, luciferase. Results showed that under hormone stimulations, the expressions of OECs promoter regulated by hormones were similar to the expression of OVA mRNA in OECs, but there was no hormonal effect in 293T cells. In conclusion, we successfully established a primary culture system for Japanese quail oviductal epithelial cells, and this system can be applied to study the evaluation and regulation of the OVA promoter for exogenous protein production.
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Arntfield, Susan D. "Microstructural and rheological properties of protein networks from ovalbumin and vicilin." 1989. http://hdl.handle.net/1993/16794.
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Tsai, Ching-Hwa, and 蔡青樺. "Characterizing the effect of ovalbumin gene’s intron on its promoter activity." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/p4mace.
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碩士國立嘉義大學
農業生物技術研究所
97
A promoter is a critical DNA sequence that regulate the initiation of gene expression. The promoter not only effects the efficiency of transcription initiation, but also influenced the expressive activity of a foreign gene in transgenic animals. Ovalbumin is a major constituent of hen egg white proteins accounting for 60-65% of total protein, which apparently means the promoter located upstream of ovalbumin gene has high activity to promote the expression of ovalbumin protein. To improve the expression of downstream foreign genes, ovalbumin promoter could be constructed into the upstream of exogenous gene for high level expression in transgenic animals. Besides, recent researche reveals that intron could enhance mRNA stability and transcriptional efficiency and then increase the output of protein. Therefore, in addition to 800 bp of ovalbumin promoter sequence, the cloned ovalbumin promoter includes three exons and two introns of ovalbumin gene. In order to characterize the expressive activity and efficiency of the ovalbumin promoter, EGFP was constructed into the downstream of ovalbumin promoter as a reporter gene. Subsequently, three recombinant plasmids, pOVA2.8int-EGFP, pOVA2.9int-EGFP and pOVA2.6-EGFP, have been constructed. Liposome-sperm mediated method was used for gene transfer of pOVA2.8int-EGFP to generate transgenic chickens. Three of twelve transgenic chickens was comfirmed with transgenes by PCR analysis. By comparing the fluorescent intensity in the transfected CHO-K1 cells, pOVA2.9int-EGFP with intron2 is demonstrated to have a greater activity than pOVA2.6-EGFP without intron2.
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Wang, Ren-Tsung, and 王仁聰. "Bactericidal mechanism of carboxyl group-modified bovine serum albumin and ovalbumin." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/50865504818499138533.
Full textAbstract:
碩士國立中山大學
生物醫學研究所
103
The aim of this study is to investigate the physicochemical properties and antimicrobial activities of semicarbazide-modified bovine serum albumin (BSA) and ovalbumin (OVA). MALDI-TOF and RP-HPLC showed that modification of carboxyl groups caused an increase in molecular weight and hydrophilicity of SEM-BSA and SEM-OVA compared with those of BSA and OVA. CD spectra measurement revealed that modification of carboxyl groups caused a change in the secondary structure of BSA and OVA. SEM-BSA exhibited a growth inhibition on E. coli and S. aureus, while SEM-OVA only shows bactericidal activity against S. aureus. Destabilization of structural stability of lipopolysaccharide (LPS) or inhibition of lipoteichoic acid (LTA) synthesis promoted antibacterial activity of SEM-BSA and SEM-OVA. Propidium iodide (PI) staining indicated that SEM-BSA and SEM-OVA induced membrane permeability of S. aureus. Compared to SEM-BSA, SEM-OVA insignificantly affected the membrane permeability of E. coli. SEM-BSA and SEM-OVA induced membrane fusion and permeability of S. aureus membrane-mimicking vesicles. Unlike SEM-BSA, SEM-OVA did not damage E. coli membrane-mimicking vesicles. Both LPS and LTA suppressed membrane-damaging activity of SEM-BSA and SEM-OVA. SEM-BSA showed higher binding affinity with bacterial membrane-mimicking vesicles compare to SEM-OVA. Moreover, SEM-BSA penetrated into membrane and perturbed the membrane structure. The interacted-mode of SEM-OVA with S. aureus and E. coli membrane-mimicking vesicle differed. Taken together, the antibacterial action of SEM-BSA and SEM-OVA are related to their ability to damage bacterial membrane. The membrane-interacted mode of SEM-OVA may elucidate its inability to display antibacterial activity against E. coli.
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Kuo, Chieh Ying, and 郭杰穎. "To study the effects of tomatidine on ovalbumin-sensitized asthmatic mice." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/81909174363712727768.
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Soares, Ana Sofia Augusto. "Strategies for reducing the allergenic capacity of ovalbumin using phenolic compounds." Master's thesis, 2015. http://hdl.handle.net/10400.6/5964.
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Egg allergy, is an IgE mediated reaction and is one the most common food allergies, mainly in infants and young children. Eggs present allergens both in the egg white and yolk, but most of the allergenic proteins are found in egg white where ovalbumin (OVA) is the main protein and one the main allergens identified. The industry has sought to implement methods and techniques for reducing the allergenicity, however, this task has not been successful. Phenolic compounds bind to peptides and proteins and can promote alteration of their native conformation and thereby alter its allergenicity.
Thus, this work aimed to study the effect of phenolic compounds on the native structure of OVA, using circular dichroism and fluorescence techniques. OVA was treated with different phenolic compounds (Gallic, Caffeic, Ferulic, Chlorogenic and Tannic Acids, Resveratrol and Quercetin) and the changes in their secondary and tertiary structure were studied as well as, the type of interactions involved through the calculation of the thermodynamic parameters, and complex formation between the OVA and phenolic compounds under study. Also, the Kb was evaluated.
The results indicated that all tested phenolic compounds bind to OVA and the hydrophobic interactions are the main kind of interaction involved. The structure of OVA is affected by the binding with phenolic compounds, mainly with Gallic and Tannic Acid, at the level of ß-sheet and ß-turns content. Thus, these phenolic compounds are likely to affect epitopes of OVA and hence its allergenicity.
This study is a good indicator that the application of phenolic compounds can promote changes in egg proteins which may influence egg allergenicity.As alergias alimentares, um problema de saúde pública, são causadas por respostas imunológicas anormais a componentes dos alimentos (alergénios), nomeadamente proteínas. As alergias mais comuns são as mediadas por imunoglobulinas E (IgE), denominadas tipo I, e caraterizam-se por reações de hipersensibilidade que podem ocorrer entre menos de um minuto ou várias horas após a ingestão do alimento que contém o(s) alergénio(s). A alergia ao ovo, mediada por IgE, é uma das formas mais frequentes de alergia alimentar em crianças, sendo a maioria reativa à clara do ovo. A ovalbumina (OVA) é a principal proteína da clara do ovo e apresenta uma elevada percentagem de reações alérgicas. A indústria tem procurado aplicar métodos e técnicas para diminuir a alergenicidade dos ovos, contudo, como em geral os alergénios possuem propriedades que lhes conferem estabilidade e resistência, tal tarefa não tem sido bem sucedida. Os compostos fenólicos, existentes na fruta e vegetais, ligam-se a péptidos e proteínas podendo promover alteração da sua conformação nativa e, deste modo, alterar a sua alergenicidade. Assim, este trabalho teve como objetivo estudar os efeitos dos compostos fenólicos na estrutura nativa da OVA, utilizando técnicas de dicroísmo circular e de fluorescência. Para tal, tratou-se a OVA com diferentes compostos fenólicos (Ácidos Gálico, Cafeico, Ferúlico, Clorogénico e Tânico, Resveratrol e Quercetina) e estudaram-se as alterações ocorridas na sua estrutura secundária e terciária, os tipos de interações envolvidas, bem como a formação de complexos entre a OVA e os compostos fenólicos em estudo. Os resultados obtidos a partir do cálculo de Kb, ?H e ?S evidenciaram a formação de complexo entre cada composto fenólico e a OVA, sendo as interações hidrofóbicas o principal tipo de interação envolvida. As alterações na estrutura terciária foram verificadas ao nível dos resíduos de triptofano com todos os compostos fenólicos, exceto com o Ácido Gálico e Quercetina onde não foram evidentes. Quanto à estrutura secundária, observaram-se alterações significativas com os Ácidos Gálico, Cafeico e Tânico. Estas alterações verificadas na conformação nativa, após tratamento da OVA, poderão eventualmente promover a redução da sua alergenicidade. Através dos resultados também podem ser sugeridos os ácidos Gálico e Tânico como potenciais promotores da redução da alergenicidade, visto que foram os únicos que promoveram alterações (diminuição) no conteúdo de folha-ß e voltas-ß, onde é estimado estarem as principais zonas alergénicas (epítopos) da OVA. Por conseguinte, este estudo poderá ser um bom indicador na aplicação de compostos fenólicos para promover alterações na alergenicidade do ovo, bem como o potenciamento de um novo método para produção de ovos hipoalergénicos. Para tal, propõe-se como perspetiva futura deste trabalho, um estudo para testar a diminuição da alergenicidade do ovo completo com compostos fenólicos, através de ensaios imunoenzimáticos utilizando soro de doentes alérgicos ao ovo.