Dissertations / Theses on the topic 'OTI'
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Střecha, Ladislav. "Kabina osobního výtahu OTI 630/0,63." Master's thesis, Vysoké učení technické v Brně. Fakulta strojního inženýrství, 2009. http://www.nusl.cz/ntk/nusl-228820.
Full textSambiéni, Coffi. "Le Proto-Oti-Volta-Oriental essai d'application de la méthode historique comparative." Köln Köppe, 2004. http://deposit.ddb.de/cgi-bin/dokserv?id=2657445&prov=M&dok_var=1&dok_ext=htm.
Full textSchwarz, Anne. "Aspekte der Morphosyntax und Tonologie im Buli." Doctoral thesis, Humboldt-Universität zu Berlin, Philosophische Fakultät III, 2007. http://dx.doi.org/10.18452/15715.
Full textThis work deals with different aspects of the morphosyntax and tonology in Buli, a Gur language of the Niger-Congo-Phylum, which is spoken in Ghana (Upper East Region near the border to Burkina Faso) and has so far been described insufficiently. The grammatical description provides an extensive insight into the linguistic system of Buli and contains chapters concerning the following topics: phonology, noun class system, pronominal system, complex noun phrases and nominal syntagmata, and verb system. It is primarily based on the Buli variant spoken in Wiaga and supplemented by reference to other dialects as well as to related Gur languages, for instance with regard to tonal divergencies in noun class suffixes and in associative constructions. Following an autosegmental model, the tonal study does not aim at theoretic enhancement, but results directly from the need of an adequate description of the synchronic language in which tone plays an important role in lexicon and grammar. Besides regular Tone-Sandhi (Low tone spreading), interesting phenomena at the phonology/syntax interface as well as at the phonology/semantics-pragmatics interface were observed – e.g. some verb forms displaying tonal agreement with the discourse role of the subject (+/- discourse participant, i.e. 1st / 2nd vs. 3rd person) or the appearance of a boundary tone on utterance-final morphemes, including the noun class suffixes of indefinite nouns and the enclitic object pronouns at the verb. In the course of the morphosyntactic analysis, a typologically remarkable nominal compound type was identified that is distinguished by structural features as well as by specific possessive semantics and can be assumed to be involved in the development of attributive (qualifying) adjectives and one of two numerals with the meaning ‘one’.
Broomfield, John S. "Policing and performing gay sexualities : how do gay men neg(oti)ate their sexual identities in the workplace and how does occupational setting frame these processes? : a comparative study into the working lives of gay male police officers and performers." Thesis, University of Warwick, 2011. http://wrap.warwick.ac.uk/100740/.
Full textFompeyrine, Jean. "Propriétés structurales et magnétiques de quelques phases dérivées de la perovskite dans les systèmes La-Ti-O et Ba(M,M') - F-Cl (M,M'élément 3 D)." Phd thesis, Université Sciences et Technologies - Bordeaux I, 1995. http://tel.archives-ouvertes.fr/tel-00148927.
Full textMartinek, Václav. "Rozpoznávání hudebních coververzí pomocí technik Music Information Retrieval." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2021. http://www.nusl.cz/ntk/nusl-442587.
Full textKnudsen-Baas, Per Harald. "OTN switching." Thesis, Norges teknisk-naturvitenskapelige universitet, Institutt for telematikk, 2011. http://urn.kb.se/resolve?urn=urn:nbn:no:ntnu:diva-14136.
Full textSauka-Spengler, Tatjana. "Évolution structurale et fonctionnelle des homéogènes de la classe orthodenticle chez les gnathostomes." Paris 7, 2002. http://www.theses.fr/2002PA077173.
Full textAlmerud, Eva-Kersti. "Ota Pavels sagovärld : Biografiska, narratologiska och språkliga perspektiv på Ota Pavels författarskap." Licentiate thesis, Stockholms universitet, Slaviska institutionen, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-111802.
Full textSac, Hakan. "Opti-acoustic Stereo Imaging." Master's thesis, METU, 2012. http://etd.lib.metu.edu.tr/upload/12614782/index.pdf.
Full textMcKenna, Claire. "Waveguides for OFI lasers." Thesis, University of Oxford, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.437190.
Full textHuang, Fengqiong, James A. Macklin, Hong Cui, Heather A. Cole, and Lorena Endara. "OTO: ontology term organizer." BioMed Central, 2015. http://hdl.handle.net/10150/610269.
Full textBoamah, Kwame. "Minerogeny of the Pan-African Volta Basin of Ghana." Doctoral thesis, Technische Universitaet Bergakademie Freiberg Universitaetsbibliothek "Georgius Agricola", 2017. http://nbn-resolving.de/urn:nbn:de:bsz:105-qucosa-223299.
Full textSjödin, Jonas. "Building OCI Images With a Container Orchestrator : A comparison of OCI build-tools." Thesis, Umeå universitet, Institutionen för datavetenskap, 2021. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-184372.
Full textNgaha, Arapera Bella. "Te Reo, a language for M��ori alone? : an investigation into the relationship between the M��ori language and M��ori identity." Thesis, University of Auckland, 2011. http://hdl.handle.net/2292/6761.
Full textCastellanos, Onorio Olaya Pirene. "Estudio de la estabilidad termica de la ocratoxina a durante el tostado del café (Coffea arabica)." Thesis, Montpellier 2, 2011. http://www.theses.fr/2011MON20033.
Full textOchratoxin A (OTA) is a secondary metabolite produced by species belonging to the genera Aspergillus and Penicillium and this toxin has been associated with certain illnesses within nephrotoxic effects, immunotoxic, teratogenic and carcinogenic. The presence of OTA in green coffee was detected in 1974 and its transmission into the beverage was made evident in 1989. Roasting coffee is a thermal process that have an effect on the OTA content. Before 1988 it was thought that the OTA was destroyed during roasting, but after several investigations, results published are contradictory results published in the % reduction (from 0 to 100%). Several authors have established hypothesis that try explain this reduction: Isomerization of the toxin in the C3 position forming a less toxic diastereomers (Studer-Rohr et al., 1995 and Bruinink et al., 1997), Protection of grain moisture degradation OTA (Boudra et al., 1995; Blanc et al., 1998 and Stegen et al., 2001), Existence of reactions with the parent toxin coffee or rearrangements of the OTA molecule roasting temperatures (200¡ã and 250¡ãC) (Su¨¢rez-Quiroz et al., 2005). Another study on thermal degradation of pure OTA showed the formation of two less toxic compounds 14 - (R)-ochratoxin A and the 14-descarboxi-Ochratoxin A (Cramer et al., 2008).Because there are no conclusive data regarding the effect of roasting on OTA in grain and the need for scientific bases for establishing regulations for export of green coffee, the objective of this work was to study the impact of different types of roasting on the thermal stability of OTA in coffee and chemical elucidation of the transformation products.Two levels of contamination were obtained by the contamination of coffee with a strain of A. westerdijkiae (5.3 and 57.2 ppb of OTA). These lots were roasted at 230 ¡ã C using two methods: Drum rotation (TR) and fluidized bed (LF). Samples were taken every 3 min from TR and every 0.9 min for LF to quantify the residual OTA. The results showed that in roasting process by TR (slower), it was more effective than with LF in the elimination of OTA (67% and 36%, respectively, for a medium roast). The thermal degradation rate of pure OTA and of OTA mixed with the components of coffee (5 sugars, 3 amino acids, caffeine and chlorogenic acids), were determined, showing that interactions took place dependent themselves on the conditions of pH and pKa values of the components tested, in this case by influencing by the reactivity and the rate of degradation of OTA. A transformation product (TP) was observed in the chromatograms obtained from the interaction of OTA with the components of coffee. A test of alkalinization and warming of pure OTA confirmed that the TP comes from the structural modification of the OTA molecule and is not a product of interaction with the natural components of coffee. The pH and temperature showed an effect in extraction of OTA in contaminated coffee, the results show better extraction of the toxin at pH 8.5 at 60 ¡ãC. At the same pH at 20 ¡ãC, it was shown a greater formation of the transformation product.The TP was purified to carry out its chemical characterization. The chemical nature of compound transformation and spectroscopic data such as UV-Vis (¦Ëmax: 237nm), the affinity with the mobile phase of the OTA, the analysis of alkalinization (OTA regeneration phenomenon and TP) analysis of stable isotopes (SIDA's) and the mass spectrum (molecular ion M +: 420 m / z), suggest that structurally the TP of OTA during the roasting process corresponds to an analogue of OTA which retains its acidic carboxyl group and in accordance to fragmentation corresponds to the Hydroxi- Ochratoxin A (OH-OTA), as well as minor amounts of OTA and its isomers
DILEM, M. B. "Arquitetura OTN Switch Com Funcionalidades de Cross-conexão e Multiplexação Óptica e Digital em Redes OTN." Universidade Federal do Espírito Santo, 2014. http://repositorio.ufes.br/handle/10/9647.
Full textA demanda de novas tecnologias de transporte de dados cada vez mais velozes e com garantias de QoS (Quality of Service), ocasionadas pelo crescimento do tráfego da Internet, traz um grande desafio na área de redes de telecomunicação. As redes ópticas de transporte se apresentam como uma solução para as elevadas taxas de transmissão. No intuito de tornar as redes de núcleo mais flexíveis e ajustadas à dinamicidade e heterogeneidade das demandas, a ITU-T (International Telecommunication Union Telecommunication Standardization Sector) desenvolve uma série de recomendações abordando as mais diversas características das redes OTN (Optcial Transport Network), no contexto das redes de núcleo, determinando suas funcionalidades. Esta dissertação tem como objetivo desenvolver uma proposta de arquitetura OTN Switch, com o funcionamento da cross-conexão e da multiplexação de sinais ópticos e digitais presentes nas redes OTN. Para tal, sua arquitetura basea-se nas recomendações geradas pela ITU-T, tornando-a capaz de prover interoperabilidade com equipamentos já existentes.
Jajtner, Petr. "Implementace OTP ve vybraném systému." Master's thesis, Česká zemědělská univerzita v Praze, 2016. http://www.nusl.cz/ntk/nusl-260667.
Full textSEBASTIEN, PIERRE. "Oto-rhino-laryngologie et sport." Toulouse 3, 1989. http://www.theses.fr/1989TOU31038.
Full textLy, Quyet Tien. "Les OSI françaises au Vietnam." Paris 7, 2007. http://www.theses.fr/2007PA070077.
Full textThis research is formally classified as a study of Franco-Vietnarnese non governmental relations. It deals with the evaluation of the French non-governmental organisations (OSI), one of the major contributors who has significantly invested in Vietnam as well as in many humanitarian endeavours to aid in the development since 1975. On one level, it takes into consideration these various aspects: origin, structure, resources, practices, employment conditions, and modes of intervention at the national office as well as in the field. On the other level, it indicates gratitude for the diversity study and the implemented actions taken. It also indicates the assets they have above other participants, the weaknesses that limit theirs actions, the relations they have established with other partners, and the role they play in instituting social policies for the country. The objective is to clarify the factors that constitute and affect this community, and to indicate how they can obtain the objective of thejr function in the particular Vietnamese context marked by the seizure of a Party Government and the presence of a great number of international actors. Over 300 French NGOs working in Vietnam is an impressive phenomenon. Their contribution to the promotion of Franco-Vietnamese relations is undeniable, However, this motivated and competent community has suffered from jnsufficient attention from the political protagonists of the two countries. There are many things that can be done by both the public authorities and the non governmental organisations to make French help more efficient and to give an exemplary image of the cooperation between a developed country and a developing one
Kabusheva, Sabina. "Transformation of Telco business strategy driven by over-the-top services such as WhatsApp, Skype and Netflix: The case of the Czech Republic." Master's thesis, Vysoká škola ekonomická v Praze, 2015. http://www.nusl.cz/ntk/nusl-206054.
Full textChraïbi, Chakib. "Contribution au test de protocoles OSI." Grenoble 2 : ANRT, 1987. http://catalogue.bnf.fr/ark:/12148/cb37603984f.
Full textChen, Yan. "Traitement transactionnel dans un environnement OSI." Grenoble 2 : ANRT, 1988. http://catalogue.bnf.fr/ark:/12148/cb376126461.
Full textFayn, Marion Hazama Danee. "'Ori Tahiti : la danse à Tahiti /." Pirae (Tahiti) : Au vent des îles éd, 2007. http://catalogue.bnf.fr/ark:/12148/cb41096768g.
Full textNotice réd. d'après la couv. Bibliogr. p. 78-79. Glossaire.
Sareh, Pooya. "Symmetric descendents of the Miura-ori." Thesis, University of Cambridge, 2014. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.708263.
Full textChraïbi, Chakib. "Contribution au test de protocoles OSI." Bordeaux 1, 1987. http://www.theses.fr/1987BOR10553.
Full textVincent, Christophe. "Syndrome branchio-oto-renal : bases moleculaires." Paris 6, 1998. http://www.theses.fr/1998PA066643.
Full textModiri, Nasser. "Monitoring and management of OSI networks." Thesis, University of Sussex, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292776.
Full textŠimko, Marek. "Současné trendy na OTC derivátových trzích." Master's thesis, Vysoká škola ekonomická v Praze, 2015. http://www.nusl.cz/ntk/nusl-206356.
Full textTakeuchi, Leonard Yasuhiko. "Study of OSI protocol processing engines." Thesis, University of British Columbia, 1991. http://hdl.handle.net/2429/30104.
Full textApplied Science, Faculty of
Electrical and Computer Engineering, Department of
Graduate
Coada, Paul, and Erkut Kaya. "Implementing Erlang/OTP on Intel Galileo." Thesis, KTH, Skolan för informations- och kommunikationsteknik (ICT), 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-177510.
Full textIntel Galileo är ett utvecklingskort som bygger på Arduinos succe. Den kommer med en kraftigare processor jämfort med Arduino Uno, och den har möjlighet att kunna köra GNU/Linux. Den har också en port för att kunna kopplas till internet och på så sätt kommunicera med andra enheter. Programmeringsspråket som rekommenderas för Intel Galileo är densamma som används för Arduinos utvecklingskort. Det finns däremot en möjlighet att kunna kombinera utvecklingskortet med ett programmeringsspråk som kan erbjuda mer funktionalitet och fortfarande vara enkelt. Vårt val hamnade på Erlang för den är ett funktionellt språk och har möjlighet att hantera olika processer. Tanken är att kunna behandla olika komponenter kopplade till utvecklingskortet som processer, som kan kommunicera med andra komponenter och med internet. Projektarbetet bestod av att undersöka ifall det är möjligt att kunna kombinera Erlang/OTP med Intel Galileon samt skriva en guide för hur implementeringen gick till. Att kombinera de två var lyckat och det öppnar upp möjligheter för fortsätta arbeten och försök.
Schreiner, Nadine. "Vom Erscheinungsbild zum "Corporate Design" Beiträge zum Entwicklungsprozess von Otl Aicher /." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=976457458.
Full textVolpe, Nathalie. "Naevus d'ota : a propos de deux cas ; revue de la litterature." Aix-Marseille 2, 1992. http://www.theses.fr/1992AIX20004.
Full textUpcraft, Lee Mark. "Modelling collisionally pumped X-ray lasers in optically field ionised noble gases." Thesis, University of York, 2002. http://etheses.whiterose.ac.uk/10791/.
Full textThéolade, Anne. "Le syndrome Bor ou branchio-oto-rénal : gravité potentielle de l'atteinte rénale, à propos de trois observations." Université Louis Pasteur (Strasbourg) (1971-2008), 1990. http://www.theses.fr/1990STR1M061.
Full textDíaz, José Antonio, and Virendra N. Mahajan. "Diffraction and geometrical optical transfer functions: calculation time comparison." SPIE-INT SOC OPTICAL ENGINEERING, 2017. http://hdl.handle.net/10150/626488.
Full textSouza, Fernanda Pasquoto de. "Tradução, adaptação e validação das escalas Obsessive-Compulsive Inventory - OCI e do Obsessive-Compulsive Inventory-Revised - OCI-R." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2008. http://hdl.handle.net/10183/13422.
Full textThe aim of the present project was to translate and adapt the scales Obsessive- Compulsive Inventory (OCI) and Obsessive-Compulsive Inventory-Revised (OCI-R) to Brazilian Portuguese and to evaluate the psychometric properties of the OCI-R, which measures the severity of the obsessive-compulsive symptoms. The project was developed in two stages: (1) the translation, transcultural adaptation and back-translation of the scales and (2) the study of reliability and validity of the questionnaire OCI-R in a clinical population and in a non-clinical sample (controls). The advantages of those scales, compared to the existent ones, are: (a) they are selfreported and (b) they produce scores for specific categories of obsessive-compulsive symptoms. In the first stage, the OCI and OCI-R, developed by the Center for the Treatment and Study of Anxiety, University of Pennsylvania, EUA were translated and adapted to the Brazilian Portuguese language. The scales were initially translated into Brazilian Portuguese by two bilingual psychiatrists and then independently back-translated by other two bilingual psychiatrists. The scales were then applied to 15 Obsessive Compulsive Disorder (OCD) patients, deliberately chosen from different educational levels, to make language adjustments. The author accepted the final version of the OCI and OCI-R scales after their back translation. Scales showed to be easy to understand and fill in by individuals and may be used with OCD patients of different socioeconomic levels. The OCI e OCI-R scales, in their version adapted to Brazilian Portuguese, can help health professionals in screening potential OCD patients assess the severity of obsessive-compulsive symptoms and the reduction of them through different treatments. The translation and the adaptation will be reported in the paper nº. 1. The second stage of the project evaluated the psychometric properties of the Brazilian version of the OCI-R in a clinical and in a non- clinical (controls) samples. The reliability and validity of the Brazilian Portuguese version of the Obsessive–Compulsive Inventory – Revised (OCI-R) were examined in a clinical sample of 64 patients with Obsessive- Compulsive Disorder (OCD), 33 patients with Social Phobia and 33 patients with Panic Disorder plus a non-clinical sample of 130 employees of a general hospital. Results indicate that the OCI-R is a valid measure for identifying and assessing the severity of the six symptom subtypes of OCD. In each sample the overall and subscale scores demonstrated moderate to good internal consistency and good convergent and divergent validity. Furthermore, in patients with OCD, the inventory showed sensitivity to Cognitive-Behavioral Group Therapy (CBGT). Our findings indicate that the Brazilian Portuguese version of the OCI-R retains the psychometric properties of its original version. These results were also compared with the results of validation of the Spanish and German OCI-R versions. That phase will be reported in the paper nº. 2. The OCI-R Brazilian Portuguese version can help health professionals and patients to screen potential people with OCD. More than this, its use can identify clinical or sub clinical OCD patients. As it is a self-report scale, it facilitates the early awareness of the disease, which is relevant because patients may take several years to seek treatment for OCD. Moreover, the use of this instrument can help to develop educational programs which could ameliorate prevention, as well as focus efforts in intervention actions.
Östberg, Mikael. "UTS: A Portable Benchmark for Erlang/OTP." Thesis, KTH, Skolan för informations- och kommunikationsteknik (ICT), 2010. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-26323.
Full textOsti, Alice. "Caratterizzazione sensoriale di oli essenziali di geranio." Bachelor's thesis, Alma Mater Studiorum - Università di Bologna, 2018.
Find full textHammar, Fredrik. "Nätverksbaserade informationssystem : Standardprotokoll ANSI Z39.50/OSI SR." Thesis, Högskolan i Borås, Institutionen Biblioteks- och informationsvetenskap / Bibliotekshögskolan, 1997. http://urn.kb.se/resolve?urn=urn:nbn:se:hb:diva-150.
Full textFrisk, Mikael. "Utveckling av OTG goggle för Scott sports." Thesis, Mittuniversitetet, Avdelningen för industridesign, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:miun:diva-23135.
Full textStrachan, Rebecca Mary. "Development of performance criteria for OSI protocols." Thesis, Leeds Beckett University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.293872.
Full textZhang, Bairong. "Formal specification and verification of OSI protocols." Thesis, University of Bristol, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337284.
Full textArnold, Matthew David. "Optimization Methods for a Reconfigurable OTA Chamber." BYU ScholarsArchive, 2018. https://scholarsarchive.byu.edu/etd/6733.
Full textJohnson, Earl E. "OTC Hearing Aids: Balancing Safety and Accessibility." Digital Commons @ East Tennessee State University, 2018. https://dc.etsu.edu/etsu-works/1975.
Full textRosenberg, Martin. "Convergence osi-rnis : une experience d'implementations portables." Paris 6, 1990. http://www.theses.fr/1990PA066300.
Full textSöderlund, Anna, and Sanna Jonasson. "OTD as a KPI in PGGI-projects." Thesis, Örebro universitet, Institutionen för naturvetenskap och teknik, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-77100.
Full textABB Power Grids Grid Integration, PGGI, levererar transformatorstationer till externa kunder, arbetet som utförs är i projektform. Alla projekt följs upp via nyckeltal, nyckeltalet OTD som ska undersökas i detta examensarbete är i form av 17 stycken standardiserade milstolpar satta från ABB för att stötta PGGI-projektens leveranssäkerhet. Problemet som ska redas ut är den nedåtgående trenden som upptäckts i leveranssäkerheten hos de 17 milstolparna. För att ta an detta gjordes en nulägesbeskrivning samt en börlägesbeskrivning, detta visade ett gap i arbetssättet gällande de 17 milstolparna. En analys av gapet mellan nuläge och börläge genomfördes, i analysen upptäcktes brister i kunskap, förståelse, rapportering samt opålitlig data gällande de 17 milstolparna. Därefter utvecklades en plan för att föreslå hur gapet mellan börläge och nuläge ska minska för att sedan elimineras. Lean filosofi, Lean verktyg samt Statistisk Process Styrning låg som grund för de framtagna förbättringsförslagen. Den framtagna planen är tänkt att användas som grund för vidare arbete med förbättringsförslagen som tagits fram.
Zuhl, Stephanie. "Survey of Patient’s Knowledge of OTC Analgesics." The University of Arizona, 2007. http://hdl.handle.net/10150/624416.
Full textObjectives: The main objective of this study was to determine if there was a link between the amount of OTC medications consumed and the knowledge of these products. With approximately 100,000 OTC products are available to the consumer this study focused on the most common class sold over the counter, analgesics. This study also determined if there was a correlation between the elderly and their frequency and knowledge of these products. Specific demographic characteristics including gender and education level were also evaluated to determine if these factors had an impact. Methods: A 21 question survey on OTC analgesics was distributed to retail pharmacy customers. It consisted of questions on amount of OTC analgesics regularly consumed, general knowledge of these products, and basic demographic questions. The initial questions assessed the amount of OTC analgesic regularly consumed by the participant. The remaining questions were designed to determine the participant’s knowledge of these products. They were either multiple choice or true false questions covering basic information on OTC analgesics Results: It was found there was no correlation between the amount of OTC analgesics consumed and the knowledge of these products. A person who consumed analgesics on a regular basis was not significantly more knowledgable about these products then a person who had never taken them. There was also no link between age and amount of OTC analgesics taken or knowledge of these products. It was found that women have more knowledge of OTC analgesics then men. Females answered an average 63.6% of the survey questions correct, compared to males who answered 51.8% correct. This project also demonstrated there was a correlation between the amount of the participant’s education level and their knowledge of OTC analgesics. Participants who had a high school education or less, answered 53.6% of the questions correct, and those who had a college degree or post graduate answered 73.5% correct. Conclusions: Although OTC analgesics don’t require a prescription, it is still important to counsel patients taking these medications. This should be considered a necessary part of the job of a pharmacist to ensure the general population has adequate knowledge of these products and is taking them safely. These products can offer a significant benefit and improve a person’s quality of life when utilized correctly. Providing patient education can ensure this can be done.
Lo, Jeffrey Kin Hung. "Open systems interconnection passive monitor OSI-PM." Thesis, University of British Columbia, 1990. http://hdl.handle.net/2429/29418.
Full textScience, Faculty of
Computer Science, Department of
Graduate
Pons, Pujol Elisenda. "Efecte de l'oli de peix i l'oli d'oliva en el desenvolupament del càncer colorectal." Doctoral thesis, Universitat Autònoma de Barcelona, 2006. http://hdl.handle.net/10803/3790.
Full textTot i que a Groenlàndia, Finlàndia i algunes zones mediterrànies, les dietes tenen un alt contingut en greix, la incidència de càncer colorrectal és relativament baixa en comparació amb la resta de països occidentals. Una important diferència dietètica és l'elevat consum de peix i oli de peix (ric en els àcids grassos n-3, eicosapentaeonic (EPA) i docosahexaenoic (DHA)) en el cas del països nòrdics i l'oli de oliva (ric en àcid oleic C18:1, n-9) a la conca mediterrània.
La tesi es basa en la hipòtesi que "l'oli de peix i el d'oliva són capaços de modular el desenvolupament del càncer colorrectal.
Objectius: L'objectiu de l'estudi va ser avaluar els mecanismes pels quals aquests olis realitzaven aquesta modulació i la implicació dels àcids grassos presents en aquests.
Resultats: Per respondre els objectius plantejats es van utilitzar dues estratègies d'estudi. La primera es basa en estudis in vitro amb línies cel·lulars d'adenocarcinoma colorectal, Caco-2 i Ht-29. L'altra es basa en l'estudi de l'efecte dels diferents nutrients en un model animal d'inducció de la carcinogènesi colorectal.
L'oli de peix i l'oli d'oliva indueixen la diferenciació en cèl·lules Caco-2. Com a resultat de la diferenciació aquestes cèl·lules comencen a expressar els enzims Sucrasa-Isomaltasa i la Dipeptidilpeptidasa IV.
La regulació de l'homeòstasi del teixit colònic depèn d'una estreta relació entre la proliferació cel·lular i l'apoptosi o mort cel·lular programada
Mitjançant estudis d'incorporació de timidina tritiada, hem demostrat que l'oli de peix té un important efecte inhibidor de la proliferació de les cèl·lules Caco-2 i HT-29. Sembla ser, que aquests efectes són deguts als àcids grassos n-3 EPA i DHA presents en l'oli de peix. L' efecte modulador del desenvolupament del càncer colorectal per part de l'oli de peix i del d'oliva té la seva major incidència en la inducció de l'apoptosi. L'índex d'apoptosi s'ha determinat analitzant els canvis morfològics de les cèl·lules (amb tinció DAPI). L'oli de peix, l'oli d'oliva, així com el DHA, l'EPA i l'àcid oleic són capaços d'incrementar l'apoptosi de forma molt significativa. Aquests efectes estan relacionats amb una molt ràpida inhibició de l'expressió de COX-2. L'expressió de la proteïna antipopotòtica Bcl-2 disminueix després de disminuir COX-2, fet que suggereix la implicació d'aquests dos gens en els efectes antineoplàstics produïts per aquests àcids grassos. Per altra banda es va demostrar una clara implicació de la proteïna pro-apoptòtica BAX, ja que a l'administrar un inhibidor d'aquesta, es produïa una disminució significativa de l'apoptosi.
L'oli de peix i el d'oliva tenen un important efecte protector en la inducció de la carcinogènesi colorectal en animals tractats amb azoximetà. Els animals que van rebre una dieta amb oli de peix o d'oliva, com a font principal de greix, presentaven un major índex d'apoptosi. Per altra banda, l'oli de peix podria tenir un efecte preventiu al ser capaç d'incidir en la renovació de l'epiteli sa, incrementant l'apoptosi d'aquestes cèl·lules.
Conclusions: L'oli de peix és capaç de modular de forma clara el desenvolupament del càncer colorectal incidint directament en els processos de diferenciació cel·lular, proliferació i apoptosi. L'oli de peix podria tenir un efecte preventiu del desenvolupament del càncer colorrectal al modificar la regeneració de l'epiteli colònic sa. L'oli d'oliva pot modular el desenvolupament del càncer colorrectal encara que no tingui el seu efecte en la totalitat dels mecanismes estudiats.
Introduction: Colorectal cancer is one of the most frequent cancers in our country as well as in most of the occidental societies. Environmental factors play a crucial role on its development. Nutrients and in concrete fat seems to be one of the most determinant components. Although relatively high fat diets are consumed in Greenland, Finland and some Mediterranean areas, colorectal cancer incidence remains relatively low there. An important difference between these regions and the rest of the western countries is the much higher consumption of fish and fish oil (rich in the n-3 fatty acids eicosapentaenoic, C20:5 and docosahexaenoic, C22:6) in the case of the Northern countries, and fish and olive oil (containing mostly oleic acid, C18:1, n-9) down in the Mediterranean basin.
The hypothesis of our study was if "fish oil and olive oil are able to modulate the colorectal cancer development".
Aims: The objectives of this study were to evaluate the mechanisms implicated in the modulation of cancer by these oils and determine the implications of its fatty acids.
Results: In order to answer the objectives mentioned above we followed two study strategies. First, we performed: in vitro studies using adenocarcinoma cell lines, Caco-2 and Ht-29. The other one is the study of the effects of several nutrients in an animal model of carcinogenesis induction .
Fish oil and olive oil induced the differentiation of Caco-2 cells. As a result of this differentiation, the cells began to express Sucrase-Isomaltase and Dipeptydilpeptidase IV enzymes.
Homeostasis regulation of colonic tissue depends on a tightly relationship between cell proliferation and cell loss, through apoptosis.
With [3H] thymidine incorporation studies, we demonstrated that fish oil has an important inhibitor effect on the proliferation of Caco-2 and HT-29 cells. It seems that this effect is caused by EPA i DHA (n-3 fatty acids present in fish oil). The most important effect on the development of colorectal cancer by fish oil and olive oil is the induction of apoptosis. To determine the Apoptotic index we evaluated cell morphologic changes by DAPI staining.
Fish oil, olive oil, as well as DHA, EPA and oleic acid are able to increase apoptosis in a significant manner. These effects are related to a very fast inhibition of the expression of COX-2. Antipoptotic Bcl-2 protein expression decreases after Cox-2 expression inhibition, suggesting that these two genes are implicated in the antineoplastic effects. On the other hand, we demonstrated a clear implication of the proapoptotic protein BAX, because when an inhibitor of BAX was added, apoptosis significantly decreased.
Fish oil and olive oil have an important protective effect in the induction of colorectal carcinogenesis in animals which received an azoximethane treatment. Animals fed with fish oil and olive oil as a principal source of fatty acids in its diet, showed a higher apoptotic index. On the other hand, fish oil may have a preventive effect due to an acceleration of the turnover of colonic epithelia through the induction of apoptosis of these cells.
Conclusions: Fish oil can modulate the development of colorectal cancer in cellular differentiation, proliferation and apoptosis processes. Fish oil could have a preventive effect on the development of colorectal cancer modifying the regeneration of normal colonic epithelial. Olive oil could modulate the development of colorectal cancer although not having effects in all of the studied mechanisms.