Journal articles on the topic 'Other earth and related environmental sciences, n.e.c'

A new hydrazide functionalized Schiff’s base derivative, N'-(3,4-dichlorobenzylidene)-4-hydroxybenzohydrazide (I), has been synthesized using a solvent-assisted mechano-chemical grinding strategy and structurally characterized using elemental analysis, 1H NMR and crystallographic studies. The single crystal X-ray diffraction study depicts that molecule is puckered with two aromatic rings lying out-of-plane in near anti-configuration across the C=N bond. The weak interactions involved in supramolecular framework formation are Cl···O, Cl···Cl, Cl···H, Cl···N, C···H, and O···H contacts. The intermolecular O···H interaction being stronger than other dispersive interactions such as halogen bonding, interlocks the molecules in a 2D sheet-type packing. All the structure directing interactions involved in developing crystal architecture are addressed with Hirshfeld surface analysis and fingerprint plots. The energy framework analysis shows visualization of 3D topology of short contacts related to molecular packing of compound I which further clarifies the predominance of both Coulombic and dispersive energies in developing supramolecular architecture.

Cells are the basic unit of life, and cell death plays an important role in the body’s metabolism, the occurrence and development of diseases. Pyroptosis is a form of programmed cell death. Pyroptosis is significantly different from other cell death methods (such as apoptosis, necrosis, etc.) in morphological characteristics, occurrence mechanism, and mechanism of action. When a cell undergoes pyroptosis, the nucleus condenses to form a pyroptotic body, numerous pores appear in the cell membrane, the cell swells and ruptures, releasing its contents. Caspase family is a homologous and structurally similar proteolytic enzyme in cytoplasm, which selectively recognizes and cleaves peptide bonds behind downstream target aspartic acid residues. Canspase 1,4,5,11 can induce pyroptosis through different pathways. Besides caspases, gasdermin also plays an important role in pyroptosis. Gasdermins (GSDMs) are a family of functionally diverse proteins expressed in a variety of cell types and tissues. The Gasdermin family includes 6 members, of which gasdermin D is the executor of pyroptosis. Upon cleavage by activated capsese, gasdermen D can be divided into N and C segments. Among them, the N fragment can form pores in the cell membrane, leading to cell swelling, rupture, outflow of cytokines and other contents, triggering the body’s immune response, and leading to pyroptosis. The occurrence of pyroptosis can be divided into the classical pathway and the non-classical pathway. The classical pathway mainly depends on caspase-1, while the non-canonical pathway depends on the activation of Caspase-4/5/11. In addition, there are uncommon Caspase-3/8- mediated pathway and Granzyme-mediated pathway. As a way of cell death, pyroptosis is inextricably linked to disease. Inflammasomes and cytokines produced in the process of pyroptosis can trigger an inflammatory response in the body, and an excessive inflammatory response can lead to diseases, such as infectious diseases, neurological diseases, and tumors. In infectious diseases, pyroptosis is closely related to the infection of a variety of bacteria, fungi and viruses, and PAMPs and LPS can be recognized by corresponding inflammasomes and caspases, respectively, and activate the downstream pyroptotic pathways. Pathogen infection is the main way to induce pyroptosis. In cardiovascular diseases, a high-fat environment can induce an increase in reactive oxygen species (ROS), trigger endothelial cell pyroptosis, and exacerbate the development of atherosclerosis (AS). In the nervous system, cell death is involved in the pathogenesis of the progression of degenerative diseases of the central nervous system, such as Alzheimer’s disease (AD), Parkinson’s disease (PD), and stroke. In terms of tumors, pyroptosis can inhibit the occurrence and development of tumors, and at the same time, as a pro-inflammatory death, pyroptosis can form a microenvironment suitable for tumor cell growth, thereby promoting tumor growth.

Three silt loam soils from a climosequence (1000-2700 mm annual rainfall) were gradually dried from field moisture content to air-dryness at 25�C in the laboratory. Microbial C measured by substrate-induced respiration (SIR), fumigation-incubation (FI) or fumigation-extraction (FE), microbial N-flush measured by FI and FE, microbial ATP content and soil phosphatase and sulfatase activities were monitored throughout the drying period (approx. 60 h). All indices declined as the gravimetric soil water content (W) decreased until reaching air-dryness. Significant declines in the biomass sometimes occurred only following a large decrease in W, dependent on the soil. In general, when microbial C and N-flush declined, the rates of decline were linearly correlated with W. However, ATP and soil phosphatase were exponentially related to W. When expressed as a ratio of the total change in microbial indices against the total change in W for the whole drying period, the ratios were consistent between the soils. Agreement between the SIR and FE estimates of microbial C, whilst significant (r = 0 58***), was poor, especially for the low rainfall soil, although the FE C- and N-flushes correlated well (r = 0-76***). In contrast, the FI C- and N-flushes correlated very poorly (r = 0.30**) and were not significantly correlated with W or the other indices. ATP and soil phosphatase activity were strongly correlated (r = 0.89***). The reliability of the methods and the influence of soil moisture regimes on microbial survival are discussed.

ABSTRACTA comprehensive study of the ∼2200-Ma-old Kuetsjärvi Sedimentary Formation (KSF), NW Russia, was undertaken to contribute to our understanding of palaeoenvironments associated with the global perturbation of the carbon cycle between 2330 and 2060 Ma. Closely spaced drill core samples (n=95) were obtained from a 150-m-thick unit deposited in rift-bound fluvial-deltaic and shallow-water lacustrine settings with a short-term invasion of sea water. Apart from a very few de-dolomitised samples, all other carbonate lithologies are represented by Corg-free, S-poor, quartz-rich dolostones, stromatolites and travertines which have high Sr concentrations (51–1069 ppm) and low Mn/Sr ratios (2·9 ± 2·1). The carbonate succession, excluding travertines, shows high δ13C (+7·5 ± 0·6‰, n=95) with a limited variation (+5·8 to +8·9‰). Fluctuating δ18O values (10·8–20·4‰) were overprinted during diagenesis, regional greenschist-grade and later retrograde metamorphism. Several short-term stratigraphic excursions of δ13C were apparently governed by evaporation and CO2 degassing combined with pulses of12C-rich hydrothermal waters precipitating travertines. However, the 13C-rich nature of the dolostones reflects the global isotopic signal, which was modified in a shallow water lacustrine environment by evaporation, enhanced uptake of 12C by cyanobacteria, and pene-contemporaneous oxidation and loss of organic material. The best proxies to δ13C and 87Sr/86 Sr of coeval sea water recorded in the KSF dolostones are likely to be around +5–6‰ and 0·70406, respectively. The study of the KSF has shown that circumspection is necessary when attempting to model the behaviour and evolution of the global C-cycle in Deep Time. Models which purport to explain global oceanic–atmospheric evolution without first adequately accounting for the possibility that many Precambrian carbonate deposits might be non-marine, or at least influenced by non-marine fluids, should be viewed with caution

Perennial grass growth forms the basis of beef production systems in northern Australia. To improve pasture productivity the woody legume Stylosanthes has been introduced into these native pastures. However, the growth of legumes has been recognised to be a major factor in soil acidification, thereby reducing soil fertility. In order to determine impacts of Stylosanthes scabra (stylo) or Urochloa mosambicensis (urochloa) residues on soil pH, acid neutralising capacity (ANC), and C and N mineralisation, their tops and roots were incubated at a rate equivalent to 10 t dry matter/ha at 25�C for 25 days in topsoil samples of a Mottled-Subnatric Yellow Sodosol from a long-term field experiment under urochloa or under stylo cover. The amount of CO2-C released during the first 2 days of incubation was correlated with the decrease in dissolved organic C. Plant material addition immediately raised the pH and ANC relative to the control. This was related to the amount of ash alkalinity of the plant residues added to the soil. Since the ash alkalinity is a measure for the organic anion content of plant material, it was concluded that the pH buffering was due to protonation of organic anions. During incubation, net N mineralisation was only observed in the urochloa soil amended with stylo leaves. In all other treatments, N added in the residues was immobilised by microorganisms due to the high availability of easily degradable C-sources. Consequently, there was no further change in pH or ANC during incubation, since no significant amounts of H+ were produced or consumed during N conversion processes.

Assessments of soil nitrogen (N) availability were undertaken using soils sampled at 0-10 and 10-20 cm depths from 123 experimental sites where the responses of cereal crops to N fertilisers were tested, throughout the cereal zone of South Australia. Rates of N mineralisation and percentage N mineralisation, as determined by a laboratory aerobic incubation method, were related to soil properties. Mineralisable N (N mineralised during a Li-week incubation) of 0-10 cm soil varied from 14 to 121 kg N/ha with a median of 50 kg N/ha, and that of 10-20 cm soil, from 5 to 42 kg N/ha (median 19 kg N/ha). Mineralisable N in 0-10 cm soil accounted for 90% of total mineralisable N in 0-20 cm soil. The percentages of N mineralised were generally higher in 0-10 cm soil (0.8-12.5%, median 3.4%) than in 10-20 cm soil (0.4-8.3%, median 2.3%). Soil organic carbon (OC) and total N could be well estimated from each other, and fron! soil pH, bulk density, and held capacity, with coefficients of determination (R2) ranging from 0.64 to 0.78. Overall, either mineralisable N or percentage N mineralisation rate in the surface soils could be well estimated from soil OC, total N, C to N ratio, bulk density, field capacity, and pH (R2, 0.78-0.86 for mineralisable N, and 0.67-0.91 for percentage N mineralisation rate).

Despite the recent interest in biochars as soil amendments for improving soil quality and increasing soil carbon sequestration, there is inadequate knowledge on the soil amendment properties of these materials produced from different feed stocks and under different pyrolysis conditions. This is particularly true for biochars produced from animal origins. Two biochars produced from poultry litter under different conditions were tested in a pot trial by assessing the yield of radish (Raphanus sativus var. Long Scarlet) as well as the soil quality of a hardsetting Chromosol (Alfisol). Four rates of biochar (0, 10, 25, and 50 t/ha), with and without nitrogen application (100 kg N/ha) were investigated. Both biochars, without N fertiliser, produced similar increases in dry matter yield of radish, which were detectable at the lowest application rate, 10 t/ha. The yield increase (%), compared with the unamended control rose from 42% at 10 t/ha to 96% at 50 t/ha of biochar application. The yield increases can be attributed largely to the ability of these biochars to increase N availability. Significant additional yield increases, in excess of that due to N fertiliser alone, were observed when N fertiliser was applied together with the biochars, highlighting the other beneficial effects of these biochars. In this regard, the non activated poultry litter biochar produced at lower temperature (450°C) was more effective than the activated biochar produced at higher temperature (550°C), probably due to higher available P content. Biochar addition to the hardsetting soil resulted in significant but different changes in soil chemical and physical properties, including increases in C, N, pH, and available P, but reduction in soil strength. These different effects of the 2 different biochars can be related to their different characteristics. Significantly different changes in soil biology in terms of microbial biomass and earthworm preference properties were also observed between the 2 biochars, but the underlying mechanisms require further research. Our research highlights the importance of feedstock and process conditions during pyrolysis on the properties and, hence, soil amendment values of biochars.

Micronutrients copper (Cu) and zinc (Zn) have the potential to inhibit soil urease activity (UA) and reduce ammonia (NH3) emissions over long duration (8–12 weeks) but have not been tested for reducing NH3 losses from cattle urine deposited in dairy-grazed pasture soils. The objective of this study was to assess the effectiveness and longevity of Cu and Zn in reducing soil UA, for the use of these metals to reduce NH3 emissions from deposited urine by grazing cattle. A series of experiments were conducted to (i) assess the relationship between inherent Cu and Zn status and soil UA of New Zealand dairy-grazed pasture soils, (ii) determine the impact of Cu and Zn addition to pasture soils on soil UA and (iii) investigate how soil organic carbon (C) and other C-related textural and mineralogical properties such as clay content and cation exchange capacity influence the effectiveness of added Cu and Zn in reducing urea hydrolysis. The results showed significant positive correlations of soil total C and total nitrogen (N) with soil UA. However, there were no significant negative correlations of soil UA with inherent Cu and Zn levels. Similarly, addition of Cu and Zn to soil did not significantly reduce soil UA. However, when Cu was added to two different soil supernatants there was a significant reduction in hydrolysis of urea applied at 120 and 600 mg urea-N kg–1 soil. Additions of Zn achieved negligible or small reductions in urea hydrolysis after 120 and 600 mg urea-N kg–1 soil applications to soil supernatants. This result suggests that Cu can inhibit soil UA and urea hydrolysis in soil supernatants with potentially low C, clay and cation exchangeable base contents. However, the interaction of bioavailable Cu with labile soil organic C and clay particles leads to its inactivation, resulting in ineffectiveness in organic C-rich pasture soils. Although most of the added Zn did not complex and remained bioavailable, the observed levels of bioavailable Zn had limited effect on soil UA.

Sown tropical perennial grass pastures may be a means to restore soil organic carbon (C) lost by cropping with conventional tillage to the levels originally present in native grass pastures. To assess this, total organic carbon and related soil properties were measured under sown tropical pastures, conventionally cultivated cropping, and native pastures on 75 Chromosols and 70 Vertosols to 0.3 m depth in the New South Wales North-West Slopes and Plains region of Australia. The impact of several perennial pasture species on soil organic carbon was also assessed in a 6-year-old, sown pasture experiment on a previously cropped Chromosol. Soil cores in 0.1-m segments to 0.3 m were analysed for total organic carbon, total nitrogen (N), pH, and phosphorus (Colwell-P). Mid-infrared scans were used to predict the particulate, humus, and resistant fractions of the total organic carbon. Bulk density was used to calculate stocks of C, N, and C fractions. In Chromosols, total organic carbon in the surface 0–0.1 m was greater under sown tropical pastures (23.1 Mg ha–1) than conventional tillage cropping (17.7 Mg ha–1), but still less than under native pastures (26.3 Mg ha–1). Similar land-use differences were seen for particulate and resistant organic C, and total N. The proportional differences between land uses were much greater for particulate organic C than other measures, and were also significant at 0.1–0.2 and 0.2–0.3 m. Subsurface bulk density (0.1–0.2 m) was lower under sown tropical pastures (1.42 Mg m–3) than conventionally tilled cropping (1.52 Mg m–3). For Vertosols, total organic carbon in the surface 0–0.1 m was greater under sown tropical pastures (19.0 Mg ha–1) and native pastures (20.5 Mg ha–1) than conventional tillage cropping (14.0 Mg ha–1). Similar land-use effects were seen for the particulate and humus organic C fractions, and total N. In the sown pasture species experiment, there was no significant difference in total N, total organic carbon, or any C fraction between soils under a native-grass species mixture, two improved tropical grass species, or a perennial pasture legume. Regular monitoring is required to better discern whether gradual changes are being masked by spatial and temporal variation. The survey results support previous research on Vertosols within the New South Wales North-West Slopes and Plains that show sown tropical grass pastures can improve total organic carbon. Improvements in total organic carbon on Chromosols have not previously been documented, so further targeted soil monitoring and experimentation is warranted for the region.

Fatty acid-based lipids comprise a small but important component of soil organic matter. Lipids are indispensable components of soil microbes due to their function as components of membranes and as stores of energy and C. Hence, lipid composition is likely under strong selection pressure and there ought to be strong associations between lipid composition of microbial communities and environmental conditions. Associations between microbial lipids and environment likely involve an integrated combination of differences in lipid headgroups (classes) and fatty acyl chains. However, past studies examining associations between soil lipid composition and environmental conditions have focussed on fatty acids hydrolysed from polar lipids and less is known about headgroups (classes) of polar lipids. The aim of this study was to examine associations between environmental conditions changing with altitude and the intact polar and non-polar lipids of soil microbial communities. We used two altitudinal transects, both spanning from forest through to above the alpine treeline, but separated from one another by ~700 km. Liquid chromatography-mass spectrometry identified 174 intact lipids to the level of class and sum composition. Approximately half of the pool of fatty acid-based lipids was accounted for by two classes of non-polar lipids (diacylglycerol and triacylglycerols), while the other half was dominated by three classes of polar lipids (phosphatidylethanolamine, phosphatidylcholine and diacylglyceryl-N,N,N-trimethylhomoserine). There were large differences among sites in the relative amounts of lipid classes. For example, diacylglyceryl-N,N,N-trimethylhomoserine varied among sites from 5 to 41% of the polar lipid pool, phosphatidylcholine from 31 to 60% of the polar lipid pool, and diacylglycerols from 9 to 53% of the total non-polar pool. Relationships of lipid composition with altitude were weak or differed between transects, and pH was the variable most strongly associated with lipid composition. Variation among sites in the relative abundance of phosphatidylcholine were positively associated with pH, while relative and absolute abundance of diacylglycerol was negatively related to pH. We suggest that the accumulation of diacylglycerol at low pH represents slowed hydrolysis and/or microbial utilisation. A large fraction of variance among sites in lipid composition remained unexplained, which highlights the need for additional research on processes leading to production and consumption of fatty acid-based lipids.

Abstract Background Clinical Practice Guidelines (CPGs) are integral during a pandemic, offering guidance to clinicians through uncertainty. Existing literature has established that the need for rapid publication of CPGs during previous infectious disease outbreaks resulted in less rigorous guidelines. CPGs were rapidly developed since the onset of the pandemic in December 2019, providing guidance in gastrointestinal (GI) endoscopy, an area where COVID-19 may pose risk of transmission. Aims To evaluate the quality of GI endoscopy guidelines developed during the COVID-19 pandemic and to compare these with (a) endoscopy CPGs developed prior to the pandemic; (b) CPGs for other endoscopic topics unrelated to COVID-19; and, (c) non-endoscopic CPGs published during the pandemic. Methods We systematically searched Medline, Embase and Scopus for CPGs published by GI societies from January 1, 2018 to December 31, 2020. A grey literature search was conducted. Two authors screened full-texts. In this interim analysis, CPGs were grouped based on publication year: before 2020, or 2020. Endoscopy CPGs published in 2020 were categorized as COVID or non-COVID related. Two authors independently assessed the CPGs using the AGREE II tool, consisting of six domains for evaluating guidelines. A domain score of 60 was set as a threshold to indicate good quality. Results There were 70 endoscopy guidelines and 27 CPGs focused on other GI topics. The mean overall scores were 69% (±12%) for endoscopy CPGs published before 2020 (n=28), and 51% (±23%) for CPGs published in 2020 (n=42). For individual AGREE II domains, mean scores for pre-2020 CPGs ranged from 33.11 (±17.39) in Applicability to 81.55 (±10.37) in Clarity of Presentation. For CPGs published during COVID-19, mean domain scores ranged from 34.18 (±10.52) in Applicability to 75.26 (±13.85) in Clarity of Presentation. 21 of 42 CPGs published in 2020 were related to COVID. Mean overall scores were 35% (±20%) for COVID-related CPGs and 67% (±13%) for non-COVID-19 CPGs. For COVID-19 CPGs, scores ranged from 27.88 (±20.31) in Rigour of Development to 69.58 (±10.81) in Scope and Purpose. For non-COVID CPGs, the scores ranged from 37.30 (±8.93) in Applicability to 84.52 (±5.93) in Clarity of Presentation. Conclusions The difference in overall scores between COVID-19 endoscopy CPGs and non-COVID endoscopy CPGs may suggest that the urgency to disseminate COVID-19 information decreased CPG quality or completeness of reporting. This interim analysis is limited by the lack of distinction between peer-reviewed CPGs and non-peer reviewed recommendations. Given the importance of CPGs in clinical decision making, it is important to ensure that the rapid development of guidelines does not compromise quality and rigour. Funding Agencies None

Relationships between changes in soil pH and changes in other soil chemical properties were examined using data from a survey of 107 pasture sites from across Victoria. At each site, soil samples (0-5, 5-10, 10-15 and 15-20 cm depths) were taken from the pasture and an adjacent undisturbed (reference) area for chemical analysis. Changes in soil chemical properties were inferred from differences between pasture and reference soils. Increases in extractable Al and extractable Mn and decreases in the sum of exchangeable cations were associated with decreases in pH. Changes in soil organic C, total soil N and total soil P were not associated with changes in pH but were related to pasture composition at each site. Increases in total soil P and exchangeable Ca, and decreases in exchangeable Mg were partly attributed to the application of superphosphate. Decreases in electrical conductivity are discussed in relation to vegetation and salinization.

Relationships were sought between infiltrability and the properties of hundreds of surface soils (pedoderms) sampled across Namibia and western South Africa. Infiltrability was determined using a laboratory method, calibrated against a rainfall simulator, which measures the passage of a suspension of soil in distilled water through a small column packed with the same soil. Other properties determined were EC, pH, water-soluble cations and anions, ammonium acetate-extractable cations, organic C, total N, a 7-fraction particle size distribution, water-dispersible silt and clay, and clay mineral composition. Our objective was to ascertain whether general principles pertaining to infiltrability can be deduced from an analysis of a wide diversity of soils. To achieve this we compared correlation analysis, generalised linear models (GLMs), and generalised additive models (GAMs) with a segmented quantile regression approach, in which parametric regression lines were fitted to the 0.9 and 0.1 quantile values of equal subpopulations based on the x variable. Quantile regression demarcated relational envelopes enclosing four-fifths of the data points. The envelopes revealed ranges for soil properties over which infiltrability is potentially maximal (spread over a wide range) or predictably minimal (confined to small values). The r2 value of the 0.9 quantile regression line was taken as an index of reliability in being able to predict limiting effects on infiltrability associated with a variety of soil properties. Prediction of infiltration was most certain from textural properties, especially the content of water-dispersible silt (r2 = 0.96, n = 581), water-dispersible clay (0.88, n = 581), very fine sand (0.86, n = 174), and medium sand (0.84, n = 174). Chemical properties such as EC, sodium status, organic C content, and clay mineralogy were less clearly related to infiltrability than was texture. The role of fine-particle dispersion in blocking pores was highlighted by the stronger prediction in all statistical analyses provided by the water-dispersible as opposed to total content of silt and clay. All the statistical analyses revealed a probable skeletal role of medium and fine sand fractions in shaping pores and a plasmic (mobile) role of finer fractions in blocking pores. A noteworthy discovery was an apparent switch in role from skeletal to plasmic at a particle diameter of about 0.1 mm (i.e. between fine and very fine sand).

Analytical data for K, Na, Ca, Mg, Al and Fe on acid extracts of samples from 52 profiles from Fraser Island, south-eastern Queensland, were used to investigate soil development in a chronosequence of podzol soils on sand, dating from mid-Pleistocene to the present. The variation observed could be accounted for by two factors, one associated with K, Fe and Al, and the other with Na, Ca and Mg. The first was most closely associated with the podzolisation process involving the weathering of minerals and the removal of the products by leaching and the deposition of Fe and A1 in the B horizon (a katamorphic factor). The second was seen as a biotic factor related to surface accumulation by the present-day vegetation. In the A horizon, both factors were related to age, implying that the soils become more impoverished with increasing age. The biotic factor, which was strongly related to the C and N contents, was also related to depth, decreasing exponentially to approximately 1.5 m, implying that this was the limit of the effect of the modern plant cover on the soil. In the B horizon, factor scores for the katamorphic factor increased markedly, indicating an increase, but not necessarily an accumulation, of K, Fe and Al. There was no relationship, however, with depth, age or carbon content. Factor scores related to the biotic factor showed a significant relationship with age, with Ca and Mg accumulating to a lesser degree in older B horizons. Several lines of evidence indicate that spodic horizons at depths greater than 1.5 m are not now in equilibrium with the surface horizons.

The effect of reduced cultivation on the chemical fertility of three agricultural soils important in Western Australia was investigated. The experiment compared the effect of different tillage systems for continuously cropping wheat on the distribution of extractable P, extractable K, total N, organic C and pH for soils ranging from a sand to a sandy clay loam. Three tillage systems were applied (conventional cultivation, direct drilled with a combine, direct drilled with a triple disc drill) and the distribution of nutrients was measured to a depth of 25 cm. Developments were monitored for a period of 9 years. Clear differences between treatments were evident within the first 3 years of the experiment. Relative differences between treatments remained constant after this time. The concentrations of extractable P, extractable K, total N and organic C in the soil were all higher with direct drilling than with conventional cultivation, regardless of soil type or environment. The concentration of these elements declined in all treatments throughout the duration of the experiment. Major differences between treatments occurred in the surface 5-10 cm of the soil, although trends differed slightly depending on the element measured. Ammonium nitrate application substantially reduced the pH of the sandy soil, regardless of tillage treatment, but had no effect on the other soil types. Conventionally cultivated sandy and sandy clay loam soils also had a slightly higher pH than the direct drilled soils. The yield of plants was not directly related to the concentration in the soil of any elements measured. Accumulation of nutrients near the surface indicated that, if the surface soil is dry, then nutrients may be less available to plants established with direct drilling than with conventional cultivation. Further research is needed to establish whether present 'soil testing-P recommendation' relationships, based on conventional cultivation systems, are appropriate under direct drilling systems in Western Australia. Changes in pH with different tillage systems may have a bearing on fertilizer application strategies. Finally, the decline in organic C with conventional cropping indicates that the structure of soils in this experiment remains fragile even with direct drilling.

This study is a development research that aims (1) to develop a guided inquiry-based learning module in increasing learning independence and learning outcomes (2) to produce a guided inquiry-based e-Module which is valid, practical, and effective in increasing learning independence and learning outcomes. The development model used in this study is the ADDIE development model. The guided inquiry-based e-Module developed had gone through the validation stage by experts so that based on the validation results it is declared valid and based on the results of trials in class it is declared practical and effective. e-Modules and other research instruments are stated to have met the criteria for validity in valid category. Based on the research data, the e-Module have met the practicality criteria because the practicality test is related to: (a) the implementation of the guided inquiry-based learning e-Module with the average value of M=1.79 in the interval (1.5≤M≤2 ,0) with implemented entirely category; (b) positive teacher’s response with a percentage of 89% in very practical criteria; and (c) very positive students’ responses with a percentage of 81% in very practical criteria. The guided inquiry-based e-Module is stated to be effective because it has met the effectiveness criteria with the results: (a) increasing students' learning independence from before treatment 57.02% to 79.63% after the treatment; (b) the learning outcomes meet the effective criteria for the n-gain value with an increase in learning outcomes by 0.78 in high category. e-Module was developed with the help of 3D Pageflip Professional software. The guided inquiry-based e-Module that was developed includes elements consisting of: (1) cover, containing the identity of the colloid e-Module, (2) instructions for using the e-Module, (3) KD and learning indicators, (4) a brief Colloid material at certain meetings, (4) learning videos, and (5) learning activities.

This study aimed to examine the implementation pattern of the constructivist theory in limited face-to-face learning (PTMT). The method used was descriptive qualitative with the subject of teachers in the Malang City area. Data was collected by using a survey distributed online through Google Forms. The results showed that the implementation pattern ofconstructivist theory in limited face-to-face learning (PTMT) was carried out through 5 (five) base of learning implementation, namely the application of problem-based learning, the application of project-based learning, the use of digital-based teaching materials and media, the application of psychosocial learning and contextuallearning. Teachers carried out limited face-to-face learning by applying constructivist theory massively and gradually, starting from planning, the process of learning activities, and learning evaluation. In addition to the use of media and 5M learning models in this study, the application of constructivist theory in PTMT learning aimed to help students become more active and teachers as moderators be more innovative. Learning process carried out at the elementary school level was only 50 percent attendance at the maximum. The implementation of student-centered learning showed 45% was very good, 50% was good, 2.5% was good, 2.5% was not good. The results showed that the inhibiting factor for student-centered implementation was the limited duration of time in learning. On the other hand, there were also restrictions on interactions carried out in the classroom so that teachers experienced a decline in exploring the learning process. Keywords: constructivism theory. limited face-to-face learning, elementary school. References: Adiyono, A. 2021. Implementasi Pembelajaran: Peluang dan Tantangan Pembelajaran Tatap Muka Bagi Siswa Sekolah Dasar di Muara Komam. 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The concept of responsibility accounting accompanies the concept of responsibility center which means that any authority given to a manager over a business unit must be accounted for by that authority, especially the authority in achieving financial targets, both revenue and expenses. The concept of responsibility center and responsibility accounting are management tools to ensure that the entrusted manager truly upholds the trust by exercising the given authority and must dare to take responsibility for that authority in the future. This concept also emphasizes that a manager cannot be held responsible by his superiors for more than the authority given to him. More than that, this concept aims not to let in the management of the organization, managers throw responsibilities at each other or "scapegoat" others to escape the responsibilities of the work unit they lead. One of the functions of accounting is a tool for financial accountability, indeed the study of responsibility accounting is part of the realm of management accounting, but in a broader sense, the financial accountability of public companies can also be called "public accountability accounting". In this section, the irony occurs, accounting as a financial accountability tool sometimes "slips" into a manipulation tool. However, research at the level of society-based economy practice seems to show that the light of responsibility accounting remains illuminating and can even be referred to as “beyond responsibility accounting", because accountability accounting is not only interpreted as a material aspect, not limited to accountability to interested parties but accountability is related to religious values, namely, all things in this world will be accounted for in the future to God. 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The book is intended for students studying medical and biological specialties. CHAPTER I. EPIGENETICS INTRODUCTION The science of epigenetics looks at the mechanisms of molecular modifications of histones and DNA that can regulate gene activity without affecting the nucleotide sequences in the DNA molecule. Recognized epigenetic regulators are DNA methylation, post-translational modifications of histones, and non-coding RNAs (nkRNAs). One of the most important differences between eukaryotic cells and prokaryotes is the presence of a complex nucleo-protein chromatin complex in eukaryotes. It is in this form that the DNA molecule is stored in our cells. On the one hand, the complex structural organization of chromatin provides a compact arrangement of DNA in the cell nucleus. On the other hand, chromatin is directly involved in the process of regulating gene expression. At the same time, the nucleosome depicted in Fig. 1 (a structural and functional unit of chromatin) is considered as a key component in the processes of regulating gene expression. The nucleus of the nucleosome is 8 histone proteins (octamers). The nucleus of the nucleosome consists of two copies of each of the histone proteins H2A, H2B, H3 and H4. The DNA chain, which includes 147 nucleotides, folds 1.65 times around the octamer of histones. The nucleosomes are arranged as a linear array along the DNA molecule in the form of "beads on a string". The linker section of DNA connecting adjacent nucleosomes (transcriptionally inactive) is sealed with H1-histone protein. The length of the linker section is 30 nm. Moreover, the site of the beginning of transcription is usually located inside the nucleosome. Consequently, the nucleosome serves as a gene repressor, preventing the initiation of transcription. That is, chromatin provides a total repression of genes. In contrast, transcription becomes possible as a result of chromatin remodeling factors that enable the "dismantling" of nucleosomes or otherwise alter their structure and organization. Thus, the repression (inactivation) of genes begins with wrapping the DNA molecule around the histones in the nucleosome, and the liberation of genes from repression (activation) involves freeing DNA from binding to histone proteins and unfolding DNA by chromatin remodeling factors (Lorch Y., Kornberg R. D., 2017). Thanks to this mechanism, selective expression of only those genes that are needed at a given time by the cell or tissue is possible. It should be emphasized that nucleosome repression extends not only to transcription, but also to most other biological processes associated with the DNA molecule, such as replication, mitotic division, repair of double-strand breaks, and maintenance of telomeres. Thus, epigenetic mechanisms control various physiological and pathological processes by regulating the expression of the corresponding genes by changing the availability of epigenetic control systems to chromatin. The scope of application of epigenetic research methods is rapidly expanding. Currently, we are witnessing the active introduction of epigenetic approaches in the field of practical medicine aimed at diagnosing and treating dangerous human diseases. CHAPTER II. TRANSCRIPTION FACTORS INTRODUCTION For the first time, the existence of transcription factors was revealed on the basis of a discovery that made it possible to establish in vitro purified RNA polymerase-II can initiate transcription on the DNA template in the presence of a cell extract (Weil P. A. et al., 1979). Further research aimed at the fractionation and identification of the general transcription factors (GTF) required to initiate transcription in vitro has identified similar factors in rats, Drosophila, and yeast and substantiated the assumption that GTFs are indeed "common" factors necessary for the expression of genes transcribed by RNA polymerase II. is highly conserved in a number of eukaryotic organisms (Matsui T. et al., 1980). We only mention RNA polymerase II because only this type of enzyme has the ability to synthesize mRNA. Whereas RNA polymerase I is responsible for the synthesis of pro-rRNA, and RNA polymerase III is responsible for the synthesis of tRNA and other non-coding cell RNAs. Meanwhile, the regulation of transcription in eukaryotes is quite complex, since it depends on chromatin remodeling complexes (Burns L. G., Peterson C. L., 1997) and covalent modification of histone proteins (Natsume-Kitatani Y., Mamitsuka H., 2016). In transcription initiation, the immediate target of GTF is a well-defined promo zone of a structural gene. In the structure of the promotra of eukaryotes, the main elements and regulatory elements can be distinguished. The main elements of the promotra (bark promoter, see Fig. 2.1) can be attributed to the site for assembling the transcription initiation complex (PIC), including the TATA sequence located above from the transcription start site (TSS ), and an initiating sequence (Inr) covering the start site. Promoters may include a TATA unit, an initiator sequence (Inr), or both (Hampsey M., 1998). A third major element, the downstream promoter element (DPE), was originally described in Drosophila and is located about 30 p.p. below TSS. The DPE promoter element appears to function in conjunction with the Inr element as a binding site for the transcription factor TFIID on non-TATA promoters. According to current research, the cellular (main) promoters of multicellular organisms that control the initiation of transcription by RNA polymerase II may contain short sequences of nucleotides called cow promoter elements (motifs) (e.g., the TATA block, the initiator (Inr), and the lower element of the cow promoter (DPE)) that recruit RNA polymerase II through a common transcription initiation mechanism (Dreos R. et al., 2021). The authors report that the classes of Promoters of Inr+DPE are not only present in the genome of Drosophila and humans and are structurally similar to each other, but may also be common to different species of multicellular organisms. The most studied element of the cow promoter is the TATA box, but the TATA box is found only in about 10-20% of multicellular cortical promoters. Therefore, along with the TATA sequence, it is necessary to name other possible key DNA sequences known as cortical promoter elements, which include: BRE, MTE, TST and DPE sequences. The two BRE (TFIIB recognition element) motifs are located either above (BREu) or below (BREd) elements of the TATA box. It should be emphasized that TBP, TATA box, and BRE demonstrate high levels of conservatism in the range from archaebacteria to humans (Kadonaga J. T., 2012). In doing so, BREu as well as BREd have both positive and negative effects on transcription activity. The downstream core promoter element (DPE) was detected in the analysis of non-TATA gene promoters in Drosophila. The MTE element (motif ten element), which is located directly in front of the DPE, was identified as an overrepresented sequence of a cow promoter called "motif 10" and then discovered, that it is a functional element of a cow promoter. The MTE and DPE motifs exhibit high conservatism in the range from Drosophila to humans, and both motifs appear to be directly recognized by the subunits of the main transcription factor TFIID, TAF proteins that resemble histone proteins in structure. In turn, the TCT sequence regulates the transcription of ribosomal protein genes in Drosophila and humans. Although there are no universal cortical promoter elements that are present in all promoters, the concept of a cow promoter of nuclear RNA polymerase II can be defined as a minimum stretch of DNA that is sufficient to accurately initiate transcription by RNA polymerase II (Kadonaga J. T., 2012; Haberle V., Stark A., 2018). It should be noted that the results of modern research will constantly supplement the list of all new components of the cow promoter, for example, DNA-replicatedrelated element (DRE), Ohler 1,6 and 7 motifs (Danino Y. M. et al., 2015; Haberle V., Stark A., 2018). According to the authors, the bark promoter may be transformed in the course of evolution. Due to this, gene expression levels can be modulated by the composition of cow promoter elements. Such modulation is directly achieved through the emergence of combinations of new elements of the cow promoter, as a result of which an additional level of transcription regulation is realized. To summarize the above facts, transcription is usually initiated at a specific position, the Transcription Initiation Site (TSS), at the 5' end of the gene. The TSS site is embedded in a bark promoter, which is a short sequence spanning 50 base pairs above and 50 below TSS. The cortical promoter serves as a binding platform for the components required to initiate transcription, including RNA polymerase II and related common transcription factors (GTFs). Regulatory elements. The cortical promoter is sufficient to initiate transcription, but such transcription has low basal activity, which can be further activated, generally by more distally arranged regulatory elements called enhancers (discussed below). Enhancers bind regulatory proteins known as transcription factors, recruit transcription cofactors, and can further enhance transcription. CHAPTER III. CELL SIGNALING PATHWAYS INTRODUCTION In a multicellular organism, the work of each cell is regulated by a large number of signals. These signals can be formed both in the organism itself, reflecting the specific needs of a living organism (metabolic state, stages of development, differentiation, reproduction), and in the form of a reaction to the effects of the external environment. The implementation of each of these signals encompasses all the biological and biochemical processes that lead from the cell's perception of the signal to the cell's response. A signal to a cell is something that is recognized by a specific receptor, which in turn can initiate a response to that signal. A receptor is a structure that recognizes a signal, interprets the specificity of a signal, and translates it into the cell in the form of intracellular signaling molecules, a cascade of protein phosphorylation, and other pathways. Thus, signaling to the cell begins as soon as the signaling molecule (ligand) binds to its receptor – a protein with a complementary structure on the transmembrane protein or inside the cell. Growth factors, hormones, cytokines, neurotransmitters, components of the extracellular matrix, etc. The chemical nature of the ligands is diverse, including small molecules such as lipids (prostaglandins, steroid hormones), proteins (for example, peptide hormones, cytokines and chemokines, growth factors)., complex polymers of sugars (for example, β-glucan and zymosan) and their combinations (for example, proteoglycans), nucleic acids, etc. Binding of the ligand induces conformational changes in the receptor and is then translated into the cell by activating cascades of secondary messengers (kinases, phosphatases, GTPases, ions and small molecules such as cAMP, cGMP, diacylglycerol, etc.). Thus, the message is transmitted from the membrane to the nucleus, where the processes of gene expression, subsequent translation and targeting of the protein to the cell membrane and other organelles are triggered. There are two main types of receptors – membrane (transmembrane) cell receptors and intracellular receptors. Membrane receptors are located on the plasma membrane and have a separate extracellular domain binding ligand, a transmembrane domain that is hydrophobic in nature, and a cytoplasmic domain. Cell surface receptors can be divided into G-protein-bound receptors, tyrosine kinase-bound receptors, and ionotropic receptors. When the ligand binds, plasma receptors undergo conformational changes in their extracellular domain and activate enzymatic mechanisms associated with the cytoplasmic domain, usually kinases, phosphatases and adapter proteins. These proteins can be covalently bound to the receptor and are capable of producing secondary messengers for subsequent signal transmission. Intracellular receptors can be nuclear receptors (estrogen receptor, glucocorticoid receptor, progesterone receptor, retinoic acid receptor, thyroid hormone receptor, etc.), cytoplasmic receptors or receptors located on the membranes of organelles (mitochondria, endoplasmic reticulum and Golgi apparatus). Thus, information (ligand) received on the cell surface (e.g., through a membrane receptor) is transformed by specific enzyme systems associated with the plasma membrane receptor and transmitted in the form of secondary messengers to intracellular targets. All of these components form the path of signal transmission to the cell. However, a certain set of effector proteins, enzymes and substrates that implement cellular signals form this signaling pathway (signaling cascade). Recently, however, there has been growing evidence that not only the signaling proteins themselves play an extremely important role in the regulation of cellular signaling, but also theso-called scaffold proteins ("platform proteins", adaptor proteins), which coordinate the assembly of multicomponent protein complexes. Scaffold proteins can bind several elements of one signaling pathway into a single complex, thereby modulating the efficiency of transmission of the corresponding signal. Binding and by bringing two or more signaling proteins closer together, these platform proteins direct the flow of information in the cell, activating, coordinating and regulating signaling events in regulatory networks (Skovorodnikova P.A. et al., 2017). According to the literature, several types of scaffold proteins have been described, which cover a wide range of functions. This group of proteins is usually divided into three main categories (Fig. 1): simple proteins that bind two functionally dependent proteins (adaptors), larger multi-domain proteins designed to bind a large number of proteins and regulate their activity by complex mechanisms (scaffold⁄anchoring proteins) and proteins specialized for initiating signaling cascades by localizing certain proteins-components of signaling pathways on the cell membrane (docking proteins) ( Buday L., Tompa P, 2010) The presence of such protein platforms increases the efficiency and selectivity of the signaling pathway, and also allows the formation of regulatory feedback. e ultimate target of cell signaling pathways are transcription factors that regulate gene expression and ultimately allow the resulting signal to be converted into a change in cellular activity (Brivanlou A. H., Darnell J. E., 2002). Most signaling pathways initiate a cascade of several intracellular signaling molecules that eventually form activation proteins or transcription repressors designed to bind to a specific DNA sequence. Eukaryotic transcription factors, like other proteins, are transcribed in the nucleus, but then their translation takes place in the cytoplasm. Signal transmission to the cell is a multifactorial system, which is based on nodular complexes of special proteins of signaling cascades. However, none of the signaling pathways in the cells work in isolation. The interaction of signaling mechanisms is inevitable in complex complexes, when the system perceives a combination of stimuli (hormones, cytokines, growth factors and pathogenic ligands), but at the same time preserves the accuracy of signal transmission (Saini N., Sarin A., 2021). It is well known that a relatively small number of signaling pathways control the development of all cell types in mammals (Brivanlou A. H., Darnell J. E., 2002). Combinations and time of action of the main signaling pathways determine decisions about the fate of the cell, including events such as cell differentiation in the process of ontogenesis (Li R., Elowitz M.V., 2019; de Roo J. J. D., Staal F. J. T., 2020) and cell malignancy (Dreesen O., Brivanlou A.N., 2007; Skovorodnikova P.A. et al., 2017). Consider some of the cell signaling pathways that are most important medically important. CHAPTER IV. MOLECULAR BIOLOGY OF THE TUMOR: MECHANISMS OF INITIATION, PROMOTION AND PROGRESSION INTRODUCTION Tumor diseases occupy a leading place, both in terms of morbidity and mortality. However, despite the advances in the study of molecular genetic patterns, many unresolved questions remain. On the one hand, the spectrum of molecular markers makes it possible to diagnose, predict the course, degree of malignancy, rate of tumor progression and predict a possible response to the therapy. On the other hand, those processes that occur at the molecular level are not characterized by stability, they are dynamic and are associated with a change in the genetic profile - the appearance of many clones of tumor cells with a different set of properties. The heterogeneity of tumor diseases simultaneously complicates the strategy of managing such patients, creating the prerequisites for further study of the molecular genetic characteristics of tumor cells.

A 1:1 co-crystal of phenazine and phenothiazine was prepared. The crystal structure was determined by using a single crystal X-ray crystallography technique. Analysis of the crystal revealed that the molecular complex crystallizes in monoclinic P21/n space group, C12H8N2·C12H9NS, a = 9.068(2) Å, b = 8.872(2) Å, c = 23.935(4) Å, β = 92.16(4)°, V = 1924.1(6) Å3, Z = 4, T = 293(2) K, μ(MoKα) = 0.182 mm-1, Dcalc = 1.310 g/cm3, 8057 reflections measured (3.4° ≤ 2Θ ≤ 46.54°), 2751 unique (Rint = 0.0559, Rsigma = 0.0618) which were used in all calculations. The final R1 was 0.0548 (>2sigma(I)) and wR2 was 0.1029 (all data). The molecules recognize each other through N-H···N and C-H···N hydrogen bonds, thus producing a tetramer unit. These units further interact with one another via C-H···S hydrogen bonds.

Abstract. The elemental stoichiometry of marine phytoplankton plays a critical role in global biogeochemical cycles through its impact on nutrient cycling, secondary production, and carbon export. Although extensive laboratory experiments have been carried out over the years to assess the influence of different environmental drivers on the elemental composition of phytoplankton, a comprehensive quantitative assessment of the processes is still lacking. Here, we synthesized the responses of P:C and N:C ratios of marine phytoplankton to five major drivers (inorganic phosphorus, inorganic nitrogen, inorganic iron, irradiance, and temperature) by a meta-analysis of experimental data across 366 experiments from 104 journal articles. Our results show that the response of these ratios to changes in macronutrients is consistent across all the studies, where the increase in nutrient availability is positively related to changes in P:C and N:C ratios. We found that eukaryotic phytoplankton are more sensitive to the changes in macronutrients compared to prokaryotes, possibly due to their larger cell size and their abilities to regulate their gene expression patterns quickly. The effect of irradiance was significant and constant across all studies, where an increase in irradiance decreased both P:C and N:C. The P:C ratio decreased significantly with warming, but the response to temperature changes was mixed depending on the culture growth mode and the growth phase at the time of harvest. Along with other oceanographic conditions of the subtropical gyres (e.g., low macronutrient availability), the elevated temperature may explain why P:C is consistently low in subtropical oceans. Iron addition did not systematically change either P:C or N:C. Overall, our findings highlight the high stoichiometric plasticity of eukaryotes and the importance of macronutrients in determining P:C and N:C ratios, which both provide us insights on how to understand and model plankton diversity and productivity.

Cosmopsaltria halmaherae n. sp. is described from Halmahera, Maluku and placed in the C. doryca group. The characters of this group are reconsidered and the relationships of its members are discussed. The C. doryca group and two other monophyletic groups of cicadas demonstrate a vicariant distribution in Maluku Utara (= North Moluccas) and Maluku Selatan (= South Moluccas).

Four new species of Melitidae (Amphipoda)are described from anchihaline limestone caves in New Caledonia. Three species are classified with Josephosella Ruffo, 1985 (J. microps n. sp., J. debilis n. sp., and J. proiecta n. sp.) and a new genus, Caledopisa, is erected for the fourth species, C. levis n. sp. The new genus is related to Victoriopisa Karaman & Barnard, 1979.

Background. The aim of this study was to assess the health-related behaviour of women practising judo and ju-jitsu in comparison with others (active and physically inactive). Material and methods. The study involved 414 women aged 18 to 30. For comparison purposes, they were divided into three groups. Group 1 (n=56) were women practising judo or ju-jitsu. Group 2 (n=166) were persons practising other types of regular physical activity and Group 3 (n=192) included those who did not engage in organized physical activity of any kind. The study used the Juczynski Health-Related Behaviours Inventory and the authors’ own survey. Results. The studied judo and ju-jitsu athletes were characterized by a high or average level of health behaviours (on average 85.87). The total score of the intensity of health behaviours achieved by amateur athletes practising combat sports and martial arts was higher than the results of the other groups (p<0.01). Conclusion. Women practising martial arts were characterized by a high overall index of health behaviours – they more often paid attention to correct eating habits and preventive health behaviours, among others. This may testify to the intellectualization of athletic and health-related training and the transfer of desired behaviours to everyday life.

We report herein the linear optical properties of some extended 2,4,6-triphenyl-s-triazines of formula 2,4,6-[(1,4-C6H4)C≡C(4-C6H4X)]3-1,3,5-(C3H3N3) (3-X; X = NO2, CN, OMe, NMe2, NPh2) and related analogues 4 and 7-X (X = H, NPh2), before briefly discussing their two-photon absorption (2PA) cross-sections. Their 2PA performance is discussed in relation to 2PA values previously measured for closely related octupoles such as N,N′,N″-triphenylisocyanurates (1-X, 5, and 6-X) or 1,3,5-triphenylbenzenes (2-X). While s-triazines are usually much better two-photon absorbers in the near-IR range than these molecules, especially when functionalised by electron-releasing substituents at their periphery, they present a decreased transparency window in the visible range due to their red-shifted first 1PA peak, in particular when compared with corresponding isocyanurates analogues. In contrast, due to their significantly larger two-photon brilliancy, 2,4,6-triphenyl-s-triazines appear more promising than the latter for two-photon fluorescence bio-imaging purposes. Rationalisation of these unexpected outcomes is proposed based on DFT calculations.

The terrestrial gross primary productivity (GPP) plays a crucial role in regional or global ecological environment monitoring and carbon cycle research. Many previous studies have produced multiple products using different models, but there are still significant differences between these products. This study generated a global GPP dataset (NI-LUE GPP) with 0.05° spatial resolution and at 8 day-intervals from 2001 to 2018 based on an improved light use efficiency (LUE) model that simultaneously considered temperature, water, atmospheric CO2 concentrations, radiation components, and nitrogen (N) index. To simulate the global GPP, we mapped the global optimal ecosystem temperatures (Topteco) using satellite-retrieved solar-induced chlorophyll fluorescence (SIF) and applied it to calculate temperature stress. In addition, green chlorophyll index (CIgreen), which had a strong correlation with the measured canopy N concentrations (r = 0.82), was selected as the vegetation index to characterize the canopy N concentrations to calculate the spatiotemporal dynamic maximum light use efficiency (εmax). Multiple existing global GPP datasets were used for comparison. Verified by FLUXNET GPP, our product performed well on daily and yearly scales. NI-LUE GPP indicated that the mean global annual GPP is 129.69 ± 3.11 Pg C with an increasing trend of 0.53 Pg C/yr from 2001 to 2018. By calculating the SPAtial Efficiency (SPAEF) with other products, we found that NI-LUE GPP has good spatial consistency, which indicated that our product has a reasonable spatial pattern. This product provides a reliable and alternative dataset for large-scale carbon cycle research and monitoring long-term GPP variations.

Global pandemic of Coronavirus Disease 2019 (COVID-19) caused by SARS-CoV-2, a novel virus which first reported in Wuhan in December 2019. It can cause severe respiratory conditions including respiratory failure. Different from adults, children usually have milder symptoms of the disease. A post-infection complication that involves multisystem organ failure in children is reported in numerous countries. It is mentioned as multisystem inflammatory syndrome in children (MIS-C) or Kawa-COVID-19, because it resembles Kawasaki Disease. The pathogenesis remains unclear, but it is presumed that hosts’ innate immune response triggered the condition. Modulating the immune response is the main target of the therapy. High doses of intravenous immunoglobulins, low doses of corticosteroids (methylprednisolone), anti-IL-1 (anakinra), antiplatelet such as aspirin can be used to treat MIS-C. Antiviral therapy is not proven to be effective and other immunomodulatory agents still needed further studies.

Shade trees are used in agroforestry pattern for production and service aspects. Cocoa-tree agroforestry pattern is expected to improve atmospheric and rhizosphere zone in cacao plantations. However, the information related to this is still quite limited. This study was conducted using a nested design, with types of shade trees as nest. Shade trees used were Falcataria moluccana, Cocos nucifera, and Cassia spectabilis, and without shade was used as control. Variables observed were light, air temperature, humidity, soil temperature, soil texture, bulk density, permeability, and moisture content. Soil chemical properties were also observed, including organic C, pH, total N, P, K, Ca, Mg, B and content of available P, K, Ca, Mg, and B. The results showed that cacao agroforestry pattern using F. moluccana and C. spectabilis was able to improve the microclimate characteristics and soil fertility. However, F. moluccana showed lower soil fertility than C. spectabilis. F. moluccana and C. spectabilis shade trees were able to optimize light plants from 33 % to 34 %, and from 38 % to 39 %, respectively. F. moluccana could provide optimal air and soil temperature of 30 ⁰C to 32 ⁰C and 27 ⁰C, respectively. Meanwhile, C. spectabilis could provide optimal air and soil temperature of 29 ⁰C to 31⁰C and 26 ⁰C to 27 ⁰C, consecutively. Cocoa-tree agroforestry pattern using C. Spectabilis shade trees could optimize soil moisture content, pH, total N, and P, and availabilities of K, Ca, Mg, and B in soil.

Background: To evaluate obstetrical and neonatal outcomes of singletons conceived after advanced assisted reproductive technology (ART) techniques: conventional IVF pregnancies (C-IVF), ejaculated sperm intracytoplasmic sperm injection (ICSI), assisted oocyte activation (AOA), in vitro maturation (IVM), and testicular sperm extraction (TESE). Methods: The subjects were 3,028 singletons who were born after fresh or frozen embryo transfer. The subjects were separated into five groups: C-IVF (n = 855), ICSI (n = 1,869), AOA (n = 42), IVM (n = 32), and TESE (n = 230). We evaluated obstetrical and neonatal outcomes calculating the adjusted odds ratio (AOR) using multivariable logistic regression analyses for fresh and frozen embryos and for cleavage and blastocyst transfer. The C-IVF group was used as a background control for the ICSI group. Moreover, the TESE, AOA, and IVM groups were compared to the ICSI group to evaluate the effects of the ICSI procedure itself. Results: The incidence of perinatal complications was significantly lower in the ICSI-fresh group (AOR = 0.29, 95% CI: 0.10–0.83, p ¡ 0.05). Regarding sex ratio, the IVM was significantly associated with sex ratio imbalance toward female in both fresh and frozen groups (AOR = 0.30, 95% CI: 0.10–0.96, AOR = 0.27, 95% CI: 0.07–0.98, p ¡ 0.05). On the other hand, there were no significant differences in preterm birth rate, low birth weight rate and congenital abnormalities rate between conventional IVF, ICSI, and the other groups. Conclusions: There were no negative effects on obstetrical and neonatal outcomes between conventional IVF and ICSI. Although this was a limited sample size study, advanced ART technologies such as AOA, IVM, and TESE also seem to have a low risk of adverse impact on obstetrical and neonatal outcomes but may have a slight impact on sex ratio.

Abstract. Ombrotrophic bogs in southern Patagonia have been examined with regard to paleoclimatic and geochemical research questions but knowledge about organic matter decomposition in these bogs is limited. Therefore, we examined peat humification with depth by Fourier Transformed Infrared (FTIR) measurements of solid peat, C/N ratio, and δ13C and δ15N isotope measurements in three bog sites. Peat decomposition generally increased with depth but distinct small scale variation probably caused by environmental changes occurred. C/N ratios varied mostly between 40 and 120 and were significantly correlated (R2 > 0.55, p < 0.01) with FTIR-derived humification indices. The degree of decomposition was lowest at the site with the least sea spray input, while the peat was most decomposed at the driest site with highest sea spray deposition. Decomposition of peat was also advanced near ash layers. Values of δ13C were 26.5 &amp;pm; 2‰ in the peat and partly related to decomposition indices, while δ15N in the peat varied around zero and did not consistently relate to any decomposition index. Concentrations of DOM partly related to C/N ratios, partly to FTIR derived indices. DOM was enriched in 13C and in 15N relative to the solid phase probably due to multiple microbial modifications and recycling of N in these N-poor environments. In summary, paleoclimatic signals may have influenced decomposition according to depth profiles of C/N ratios, δ13C values, and FTIR spectra, but the study also suggests that decomposition was also influenced by ash layers, sea spray input and other site specific factors.

A single boulder (PH 8.14), recovered from unit 8 on the Point of Head, Cow Head Peninsula, Shallow Bay Formation (Cow Head Group), contains a unique olenid-dominated trilobite fauna composed of the following taxa: Boeckaspis geordii n. sp., Leiobienvillia terranovica (Rasetti, 1954), hystricurid n. gen. n. sp., Tulepyge sp. A, Tulepyge sp. B, Tulepyge? sp. C, and Symphysurina sp. The pelturine trilobite B. geordii dominates the assemblage. Boulder PH 8.14 consists of light grey-brown packstone to grainstone. This is atypical for olenid-dominated faunas, which usually occur in dark laminated limestones and shales. The fauna is likely earliest Ordovician in age, but a latest Cambrian age cannot be entirely ruled out. Regardless, this assemblage is unique in that it is the only known olenid-dominated fauna from Cambrian–Ordovician boundary sediments in Laurentia. A phylogenetic analysis of Boeckaspis and other putatively closely related taxa ( Westergaardia Raymond, 1924 and Jujuyaspis Kobayashi, 1936) was conducted. The results of the analysis indicate that Westergaardia is not monophyletic and as such it is considered a junior synonym of Boeckaspis herein.

Abstract Background Abdominal pain is the primary cause of morbidity in many chronic GI disorders such as IBS, but the molecular mechanisms contributing to pain signaling are unclear. Stool supernatants from patients with diarrhea-predominant IBS increase the excitability of DRG neurons compared to supernatants from healthy controls, suggesting that mediators in the stool may sensitize nociceptors. Additionally, histamine has been implicated as a mediator of hypersensitivity in IBS patients. However, it is unclear if stool supernatants from constipation-predominant IBS (IBS-C) patients affect the excitability of DRG neurons. Furthermore, it is unknown if specific neuro-mediators in stool such as histamine impact the excitability of nociceptive neurons in this subgroup of IBS patients. Aims To evaluate whether IBS-C stool supernatants induce nociceptive signaling in DRG neurons compared to healthy control (HC) stool supernatants. If so, to evaluate the role of histamine 1 (H1) receptors in DRG neuron nociceptive signaling initiated by mediators in IBS-C stool supernatants. Methods IBS-C (n=5) and HC (n=2) patient stool was collected, filtered, and dissolved with Krebs solution in a 1:8 (g/v) dilution. Dorsal root ganglion (DRG) neurons from C57BL/6 mice were incubated with HC or IBS-C stool supernatant for 30 minutes. To evaluate whether histamine in stool supernatants can sensitize H1 receptors on nociceptors, DRG neurons were pre-incubated with the H1-receptor antagonist pyrilamine (1μM, 30 min) before stool supernatants. Changes in DRG neuronal excitability were recorded using perforated patch-clamp techniques to measure the rheobase (minimum input current needed to elicit an action potential) and the resting membrane potential (RMP). Results In neurons incubated with IBS-C stool supernatants (n=28) the rheobase decreased (63%) compared to healthy controls (78 ± 13.7 pA; n=6). This effect was reversed in DRG neurons pre-incubated with the H1 receptor antagonist, pyrilamine, (n=26) (77 ± 5.9 pA, p&lt;0.001) compared to neurons incubated with IBS-C supernatant alone (50 ± 5.13 pA; n=28). No changes were found in the RMP. The data were analyzed with the non-parametric Kruskal-Wallis test. Conclusions IBS-C stool supernatants increase the excitability of DRG neurons compared to HC. Furthermore, the H1-receptor antagonist pyrilamine inhibits the neuronal hyperexcitability evoked by mediators in IBS-C patient stool. These findings suggest that the neuroactive metabolite histamine may contribute to visceral pain experienced by patients with IBS-C. Further studies are needed to examine whether similar signaling to nociceptive neurons by stool supernatants occurs in other subtypes of IBS. Funding Agencies CAG, CIHR

Two species of anophthalmous, unpigmentedasellids have been found in springs and groundwaters of S.E. Siberia (Primorye region). Asellus (Asellus) primoryensis n. sp. is closelyrelated to the epigean species A. (A.) hilgendorfii Bovallius, 1886, as is the case for all stygobiont Asellus (Asellus) species previously known from the Japanese archipelago. Sibirasellus parpurae n. g., n. sp. is closely related to the microphthalmous species Asellus dentifer Birstein & Levanidov, 1952 from the Ussuri Basin (Khor region), now type-species of the new genus Sibirasellus. These two species show several original characters: body covered by numerous cuticular squamulae, mandibular palp reduced (glabrous and 2-segmented), and coxopodites of pereopods reduced and coalescent with their sternites, pointing to certain affinities with the stygobiont Japanese genus Nipponasellus Matsumoto, 1962 and probably to the epigean species of the “latifrons” group of the genus Asellus, presently restricted to arctic Siberia and western Alaska. The different asellid lines living in the Far East and Pacific North America are much more related with each other than with all other lines of the family.All these forms possess a copulatory system built on the “Asellus pattern”: Endopodite of 2nd male pleopod with a spur-shaped basal apophysis or “processus calcariformis”, an afferent spermaticopening with a labial spur or “processus cylindriformis”, and 2nd exopodite segment with a tergal or “catch lobe”. This phyletic system includes the genus Asellus Geoffroy, 1762 (the present status of which is discussed herein), its subgenera Asellus Dudich, 1925, Mesoasellus Birstein, 1951, and Phreatoasellus Matsumoto, 1962, and its related genera Calasellus Bowman, 1981, Nipponasellus Matsumoto, 1962, Uenasellus Matsumoto, 1962, and Sibirasellus n. g.

To measure the entire characteristic of p-MOSFET, we have implemented the fast Ids-Vgs technique. The latter is used to study NBTI phenomenon with measure-stress-measure method, for electric field 5MV/cm < Eox < 7.5MV/cm, and temperatures 27°C < Ts < 120°C. Measurement time has reached 10 us, and a stress-measure delay (switching time) of about a hundred of milliseconds was obtained. However, strengths and weaknesses of the implemented technique have been discussed. Furthermore, the extraction methods: transconductance (Gm), subthreshold slope (SS), and mid-gap (MG), have been implemented and discussed as well. NBTI parameter i.e. Delta Vth, n, gamma and Ea were extracted and compared to other results. A time exponent n of 0.149 has been touched. Activation energy Ea = 0.039 eV and a field factor gamma = 0.41 MV/cm for a stress time ts < 10 s have been obtained.

Abstract. Terrestrial biosphere models typically use the biochemical model of Farquhar, von Caemmerer, and Berry (1980) to simulate photosynthesis, which requires accurate values of photosynthetic capacity of different biomes. However, data on tropical forests are sparse and highly variable due to the high species diversity, and it is still highly uncertain how these tropical forests respond to nutrient limitation in terms of C uptake. Tropical forests often grow on soils low in phosphorus (P) and are, in general, assumed to be P rather than nitrogen (N) limited. However, the relevance of P as a control of photosynthetic capacity is still debated. Here, we provide a comprehensive dataset of vertical profiles of photosynthetic capacity and important leaf traits, including leaf N and P concentrations, from two 3-year, large-scale nutrient addition experiments conducted in two tropical rainforests in French Guiana. These data present a unique source of information to further improve model representations of the roles of N, P, and other leaf nutrients in photosynthesis in tropical forests. To further facilitate the use of our data in syntheses and model studies, we provide an elaborate list of ancillary data, including important soil properties and nutrients, along with the leaf data. As environmental drivers are key to improve our understanding of carbon (C) and nutrient cycle interactions, this comprehensive dataset will aid to further enhance our understanding of how nutrient availability interacts with C uptake in tropical forests. The data are available at https://doi.org/10.5281/zenodo.5638236 (Verryckt, 2021).

This study aims to determine the effect of academic procrastination on academic dishonesty in teaching and education faculties students in Indonesia. Respondents in this study were 260 students (N=260). The data collection method used the procrastination assessment scales-student (α = 0.88) and dishonesty academic scale (α = 0.93). Data analysis in this quantitative study uses Simple Linear Regression. The results of the analysis show that academic dishonesty affects student procrastination. F value = 53.078 with a significant level of 0.000 <0.05, it can be said that H0 is rejected and Ha is accepted, so that it can be said that H0 is rejected and Ha is accepted. From the output of Rsquare = 0.175, this value means that the effect of academic procrastination (x) on academic dishonesty (y) is 17.5%. In comparison, 82.5% of academic dishonesty is influenced by other variables that do not positively impact increasing academic procrastination carried out by a student. It will affect the increase in academic dishonesty in the student. So it can be said that there is a positive and significant influence between academic procrastination and academic dishonesty on students of the faculty of teaching and educational sciences at University X Purwokerto.

The periodic tables of transition metal thiophosphates MPS3, transition metal dichalcogenides MX2 and other materials, the origin of chemical elements and toxic trace elements in dried mushrooms are provided. The effective nucleus-electron attraction is proportional to the effective nuclear charge (Zeff) and inversely proportional to the effective principal quantum number (n*). The periodic arch is one of many modern visual displays that have been developed to augment the traditional periodic table of the chemical elements. The table is related with the multiparameter optimisation of N atom, nuclear magnetic resonance and everyday life. Educational activities are developed with evaluation.

Two monocelid species with simplex copulatory bulbs and post-pharyngeal ovaries have been found in E. Australia. One of them presents a combination of characters not found in any of the known genera of the Monocelididae, and the new genus Acanthopseudomonocelis is proposed for it. The new genus is characterized by the presence of four stylet-bearing accessory organs and by an internal vagina; it is related to Pseudomonocelis. The other species, Pseudomonocelis schockaerti n. sp., is characterized by a complex bursa-vagina system, and by a highly muscular penial organ and male antrum. It is closely related to P. pardii Schockaert & Martens, 1987, from Somaliland. Two possibly additional species of the genus Pseudomonocelis , not found in a mature stage, are mentioned. The new findings considerably enlarge the known distribution of the genus Pseudomonocelis.

Background: Low grade blastocyst (LGB) transfer results in varied live birth rates (5-39%). Only limited studies with small sample sizes (n=10-440) exist, due to LGB transfer being less desirable. Definitions of LGB and study designs are heterogenous, further confounding outcome interpretation. Aim: This study aimed to obtain sufficient LGB transfers to determine live birth rates, as well as allow subgroup analysis by blastocyst quality and maternal age. Method: This study was a multicentre, multinational retrospective cohort study across nine IVF clinics in China and New Zealand between 2012 to 2019. 6966 single blastocyst transfer cycles on days 5-7 (fresh and frozen) were collected; of these, 875 transfers were from LGBs (<3BB). Blastocysts with expansion stage 1 or 2 (early blastocysts) were excluded. The main outcome was live birth rate. Blastocysts were grouped according to quality grade: good-grade blastocysts (GGBs; n=3849, AA, AB and BA), moderate-grade blastocysts (MGBs; n=2242, BB) and LGBs (n=875, AC, CA, BC, CB and CC). Live birth rates were compared using the Pearson Chi-squared test. A logistic regression analysis examined the relationship between blastocyst grade and live birth after adjustment for confounders: clinic, female age, expansion stage, and blastocyst age. Results: Live birth rates for GGBs, MGBs and LGBs were 45%, 36% and 28% respectively (p<0.0001). Within the LGB group, the highest live birth rates were for grade C TE (30%) and the lowest were for grade C ICM (19%). The lowest combined grade (CC) had a 15% live birth rate (n=7/48). The odds of live birth were 2.33 (95% CI = 1.88-2.89) for GGBs compared to LGBs and 1.56 (95% CI = 1.28-1.92) for MGBs compared to LGBs following fresh and frozen blastocyst transfers (p<0.0001). The odds of live birth according to ICM grade were 1.31 (A versus B; 95% CI = 1.15-1.48), 2.82 (A versus C; 95% CI = 1.91-4.18) and 2.16 (B versus C; 95% CI = 1.48-3.16; all p<0.0001). The odds of live birth according to TE grade were 1.33 (A versus B; 95% CI = 1.17-1.50, p<0.0001), 1.85 (A versus C; 95% CI = 1.45-2.34, p<0.0001) and 1.39 (B versus C; 95% CI = 1.12-1.73, p=0.0024). Conclusion: The live birth rate for LGBs is 28%, with rates ranging between 15-31% for the different inner cell mass (ICM) and trophectoderm (TE) subgroups of LGBs. Even those in the lowest grading tier maintain modest live birth rates (15%; CC). Thus, these can be considered for transfer in women with few other options.

Abstract Background Recent evidence suggests an increasing prevalence of gut dysfunction following C. difficile infection (CDI). The accompanying prolonged antibiotic (AB) exposure likely contributes to chronic gut dysfunction and our ability to induce gut dysfunction in germ free (GF) mice colonized with microbiota from a patient with severe slow transit post CDI (PCDI) supports this notion (10.1093/jcag/gwz047.117). Furthermore, we were able to restore gut function following fecal microbial transfer from healthy murine donors. Our studies have implicated a role for macrophages in the destruction of the Interstitial Cell of Cajal (ICC) network underlying slow colonic transit in the humanized mouse model. These findings prompted us to evaluate microbiota-directed therapy in normalising gut function in this model. Aims 1)To investigate whether dietary psyllium rescues the development of slow colonic transit (SCT) through modulating host function via microbiota mediated immune mechanisms; and 2)To evaluate the mechanisms underlying the beneficial effects of psyllium Methods GF mice were colonized with either microbiota from the PCDI patient or healthy control (HC) for 3 weeks following which PCDI mice were treated with either a control diet or a 15% psyllium diet (PSY). Colonic motility was assessed before and after the diet intervention using the bead expulsion test. Stool samples were collected for microbial profiling, and short and branched-chain fatty acids (SCFA/BCFA) analysis. Colonic muscle layers encompassing myenteric plexus (MP) were collected for gene expression analysis and to evaluate activated macrophages and ICC degeneration using immunohistochemistry. Results Microbiota from a PCDI patient induced a SCT phenotype in GF mice (n=13) as compared to mice colonized with HC microbiota (p=0.0002). Psyllium rescued this SCT phenotype in mice (PCDI(n=7) vs.PSY(n=6):p=0.0014). The psyllium-induced rescue was accompanied by normalization of the ICC network and morphological alterations in infiltrating macrophages. This was supported by changes in immune-related gene expression in the MP including CD11b, NOS, Myd88, Mapk1 and NF-κB. Additionally, bacterial composition was different between PCDI and PSY group (p=0.003). SCFAs like acetic and propionic acid were increased, while BCFA like isobutyric and isovaleric acid were decreased following PSY treatment. These alterations in SCFA/BCFA were supported by fluctuations in specific bacteria like Butyricimonas, Phascolarctobacterium and Allistipes. Conclusions Our results provide evidence that chronic gut dysfunction following CDI and AB exposure is microbiota-driven. Furthermore, microbiota-directed therapy using psyllium could serve as a novel therapeutic strategy to normalize gut function via microbiota-mediated restoration of immune homeostasis in these patients. Funding Agencies W. Garfield Weston Foundation

Abstract. The nitrogen (N) removal efficiency of constructed wetlands (CWs) is very inconsistent and does not alone explain if the removed species are reduced by physical attenuation or if they are transformed to other reactive forms (pollution swapping). There are many pathways for the removed N to remain in the system: accumulation in the sediments, leaching to groundwater (nitrate-NO3- and ammonium-NH4+), emission to atmosphere via nitrous oxide- N2O and ammonia and/or conversion to N2 gas and adsorption to sediments. The kinetics of these pathways/processes varies with CWs management and therefore needs to be studied quantitatively for the sustainable use of CWs. For example, the quality of groundwater underlying CWs with regards to the reactive N (Nr) species is largely unknown. Equally, there is a dearth of information on the extent of Nr accumulation in soils and discharge to surface waters and air. Moreover, CWs are rich in dissolved organic carbon (DOC) and produce substantial amounts of CO2 and CH4. These dissolved carbon (C) species drain out to ground and surface waters and emit to the atmosphere. The dynamics of dissolved N2O, CO2 and CH4 in CWs is a key "missing piece" in our understanding of global greenhouse gas budgets. In this review we provide an overview of the current knowledge and discussion about the dynamics of C and N in CWs and their likely impacts on aquatic and atmospheric environments. We suggest that the fate of various N species in CWs and their surface emissions and subsurface drainage fluxes need to be evaluated in a holistic way to better understand their potential for pollution swapping. Research on the process based N removal and balancing the end products into reactive and benign forms are critical to assess environmental impacts of CWs. Thus we strongly suggest that in situ N transformation and fate of the transformation products with regards to pollution swapping requires further detailed examination.

In his “Slavery and Freedom”, written and published in emigration, we come across Berdyaev`s notion of “communitarianism”: “…human person must live in a free communication, free communion, in communitarianism, built on Freedom and Love”. The concept of communitarianism as akin to the concept of communalism, which is according to Berdyaev came into being as a “creative discovery” of A. S. Khomyakov. In Khomyakov`s understanding communalism is first and foremost organic unity in freedom premised in commutual love. Although one should not take the idea of communitarianism as a leading in Berdyaev`s philosophy (as distinct from communalism of Khomyakov), he granted it a special attention in his teachings. “Comunitarianism” and “communalism” are related concepts, however one should hardly take them as identical. He associates “communitarianism” with notions “collective” and “communalism”. Communitarianism is a community and communality of personalities, it is personalistic, whereas in “collective reality” the person ceases to be an ultimate value. The motif of freedom in Berdyaev`s works is far more pronounced than that of Khomyakov. That is why priorities of Khomyakov and Berdyaev in their interpretations of correlation “freedom — communalism”, “freedom — communitarianism” do not align. Berdyaev links his personalism with the idea of communitarianism: personality is communitarian and reveals its image by letting in Divine personality and other human personality. He sees Freedom as the main condition of personal becoming and even the very existence of a person as a personality; this is exactly the freedom that allows man to come to terms with his unique being. Although his assertion of communitarianism as the third freedom alongside with irrational freedom, selective (the first one) and rational freedom, enlightened (second), which he distinguishes, isn`t absolutely undebatable, it may still be acceptable.

We study the transitions between the different color states of a static quark-antiquark pair, singlet and octet, in a thermal medium. This is done non-perturbatively exploiting the infinite mass limit of QCD. This study is interesting because it can be used for future developments within the framework of Effective Field Theories (EFTs) and because it can be combined with other techniques, like lattice QCD or AdS/CFT, to gain non-perturbative information about the evolution of quarkonium in a medium. We also study the obtained expressions in the large Nc limit. This allows us to learn lessons that are useful to simplify phenomenological models of quarkonium in a plasma.

Background: Dilatation and curettage (D and C) is one of commonest procedure in the gynaecology. There are still two schools of thoughts pertaining to whether to administer antibiotic as a pre and post procedure prophylaxis or not to administer and hence study planned to evaluate the incidence of infection in patients after dilation and curettage with and without prophylactic antibiotics.Methods: Adults (>18 years) (n=100) who underwent dilatation and curettage procedure for any indication were included and patients with prior history of infection were excluded from the study. Patients were divided into 2 groups; group 1 (n=50) received prophylactic antibiotics and group 2 (n=50) received no prophylactic antibiotics as per the discretion of treating doctor.Results: Infection in both the groups was 2% and there is no statistically significant difference between the groups (p>0.349).Conclusions: Appropriate use of antibiotics can be a cost-effective strategy which can minimize the chances of development of antibiotic resistance and other side effects. Antibiotic are not necessary for pre and post procedure prophylaxis in D and C.

Azotobacter spp. Viabilities on Some Combinations of Carrier Media. The purpose of this research was to find suitable carrier media for the survival of Azotobacter spp. as a biological fertilizer, before being applied to the field. The method used in this research is explorative, by inoculating Azotobacter spp. Isolate on 4 types of carrier media, namely soil; cocopeat; wood dust and charcoal with a combination of the following: K1 (Land); K2 (soil + cocopeat); K3 (Soil + sawdust) and K4 (Soil + husk charcoal), which were repeated 3 times, so there were 12 experimental units. Analysis of N, P, K, C-organic and pH for soil media, while for cocopeat, wood powder and husk charcoal, an analysis of N and P, C-organic tissue and pH was carried out. The parameters observed were optical density at 2, 4, 6 and 8 weeks. The results showed that the survival of Azotobacter spp. in K4 carrier media (a combination of soil and husk charcoal) is more secure than other types of carrier media with optical density that has not decreased. Analysis of N and P for rice husk charcoal tissue gave the highest results, namely 1,680% and 1,351%, which were one of the supporters of this inoculant viability. Azotobacter spp. viability still guaranteed until 6 weeks incubation period, in several media, (soil + cocopeat), (soil + sawdust), and (soil + husk charcoal). The combination of media (soil + husk charcoal) gave the highest population increase at the 6 weeks incubation period.

AbstrakBogor Botanic Garden is an area designated to conserve and protect many species of plants from Indonesia and other countries as well. Most plant collections belonged to plants that continuously producing fruits. Many of fallen fruits produced by different plant species still remained optimally unmanaged and unutilized. The objective of this study was to increase the value of fallen fruits by utilizing it as raw materials for producing organic liquid fertilizer (POC). The first step of this study was conducted by collecting data about the species of fruiting plants. Liquid organic fertilizer was produced using a semi-anaerobic fermentation system with adding an effective microorganism 4 (EM 4). The results showed that there were 19 trees from 15 species such as Parmentiera cereifera, Ficus racemosa, F. fistulosa, and Dillenia indica. The fertilizers are made with a variety of supporting materials to produce liquid organic fertilizer (type A) and liquid organic fertilizer (type B). The temperature and pH had the similar value pattern during the fermentation process. Liquid organic fertilizer (type B) has a C/N ratio of 37.65, higher than liquid organic fertilizer (type A) which is 27.23. In addition, the C-organic liquid organic fertilizers (type B) was higher than liquid organic fertilizers (type A). The C-organic and N, P, and K contents have not reached the minimum technical standard for organic fertilizers that regulated on Decree of the Minister of Agriculture Number 261/KPTS/SR.310/M/4/2019. Eventually, the optimization attempts to increase the C-organic and N, P, and K contents on liquid organic fertilizer will be subsequently required.Abstract Kebun Raya Bogor merupakan kawasan konservasi tumbuhan yang memiliki koleksi berbagai macam jenis tumbuhan yang berasal dari Indonesia maupun dari luar negeri. Sebagian besar tumbuhan merupakan tumbuhan yang menghasilkan buah setiap tahunnya. Selama ini buah dalam jumlah banyak yang jatuh dari pohonnya belum termanfaatkan secara optimal. Tujuan dari penelitian ini adalah untuk meningkatkan nilai tambah limbah buah tersebut sebagai bahan dasar untuk pembuatan pupuk organik cair (POC). Studi awal dilakukan pendataan jenis tumbuhan yang berpotensi menghasilkan buah non-edible. Pembuatan POC dilakukan dengan sistem fermentasi semi-anaerobik menggunakan aktivator Effective Microorganism 4 (EM4). Hasil penelitian menunjukkan terdapat 19 pohon dari 15 jenis tumbuhan di antaranya Parmentiera cereifera, Ficus racemosa, F. fistulosa, dan Dillenia indica. Pupuk organik cair dibuat dengan variasi bahan pendukung yang berbeda menghasilkan POC A dan POC B. Pola perubahan suhu dan pH pada saat proses fermentasi relatif sama untuk kedua jenis POC. POC B memiliki rasio C/N sebesar 37,65 lebih tinggi dibandingkan dengan POC A yang hanya sebesar 27,23. Selain itu, konsentrasi Corganik POC B yang lebih tinggi daripada POC A. Nilai C-organik dan N, P, K diketahui belum mencapai standar teknis minimal pupuk organik berdasarkan SK Menteri Pertanian Nomor 261/KPTS/SR.310/M/4/2019 sehingga diperlukan optimalisasi fermentasi lebih lanjut.

The aim of the research was the review of the newest papers reporting the application of physiotherapy in scar treatment. Answers were searched to the following research-related questions: 1. Which techniques used in physiotherapy are effective in scar treatment? 2. Which tools used in physiotherapy are applied in scar treatment? 3. Was it possible to determine which approach had turned out to be the most efficient in scar treatment? The authors took advantage of the scientifically accepted medical databases online, such as: PubMed and Physiotherapy Evidence Database (PEDro). The inclusion criterion of the randomized clinical trial was based on the papers published between 2016 and May 2021. The papers on scar treatment reporting approaches different from those pertaining to physiotherapy were not included. Moreover, the papers written in languages other than English were not included either. 281 papers pertaining to this issue were found and 35 papers on physiotherapy approaches were selected. Finally, 9 papers were subjected to critical analysis. Most of the authors (n=3) reported using shockwave in their research. Next, laser treatment (n=2) or manual therapy (n=2) were applied. The fewest researchers used electrotherapy (n=1) and silicone gel (n=1).

Green gram (Vigna radiata) is considered the chief legume in Pakistan. Thus, current study was conducted to examine the ameliorating effect of phytohormones pre-treatments under salt stress on proteome profile of green gram by sodium-dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The soluble green gram seedlings proteins were resolved on 4% stacking and 12% resolving gels. The SDS-PAGE resolved 24 polypeptide bands ranging from 200 to 17kDa. Among these, 12 out of 24 bands of proteins were essentials house-keeping or growth proteins of green grams. While, 120, 114.6, 51.8, 29.1, and 22.8 kDa bands were over-expressed under 50 to 350mM salt with phytohormones treatments. The others 104.5 kDa, 99.8 kDa, 95.3 kDa, 91.0 kDa, 55 kDa, 46 kDa, and 17kDa bands were related to the GAᴣ, IAA, and SA induced tolerance. Overall 120 kDa, 114.6 kDa, 104.5 kDa, 99.8, 95.3 kDa, 51.8 kDa, 29.1 kDa and 22.8kDa bands were first time identified in the current study. The information retrieved from NCBI protein database, the resolved peptides were principally belonging to 7S and 8S vicilin, 2S, 8S, 11S, and 16.5S globulins. It is determined that seed priming with SA enhanced tolerance in green gram by rapidly synthesizing stress alleviating peptides.Key word: Cluster analysis, dendrogram, mungbean, salt stress, SDS-PAGEINTRODUCTIONVarious world-wide health concerning organization recommended the use of high graded plant protein such as legumes to prevent the risk of metabolic disorder (Hou et al., 2019). Legumes are most important protein crop on the earth. Among the legumes, the green gram is the major pulses. Its seeds are rich in superior quality storage protein, which account 85% of the total protein while, another 15% have not been broadly studied (Yi-Shen et al., 2018). The soluble storage protein comprises of 60% globulins, 25% albumin and 15% prolamins. Globulins are further divided into 3.4% basic-type (7S), 7.6% legumin-type (11S), and 89% vicilin-type (8S) (Mendoza et al., 2001; Itoh et al., 2006). Other than proteins, the green gram seeds also contain starch, fiber, phenolic compound, saponins, vitamins, calcium zinc, potassium, folate, magnesium, manganese and very low in fat that made it meager man’s meat (Hou et al., 2019). It is also a good source of green manure and fodder (El-Kafafi et al., 2015). Its root has ability to fix 30 to 50 Kg/ha atmospheric nitrogen in the soil which is essential for maintaining soil fertility (Chadha, 2010). The green gram is the valuable and the major Rabi pulse crop of Pakistan. Its cultivation area in 2016-2017 was about 179,000 hectares with seed yield of 130,000 tones. In comparison during 2017-2018, it was cultivated on 161,800 hectares land with 118,800 tones seed yield (GOP, 2018). One of the reasons of this 9% decrease in both land and productivity is the shortage of irrigated land due to soil salinity. The salinity induce oxidative bust in the mungbean cells, caused by responsive oxygen species (ROS) such as hydrogen peroxide, singlet oxygen, hydroxyl radical and superoxide radical. The ROS create hindrance in various metabolic processes of plant via interacting with macromolecules like proteins (Alharby et al., 2016). However, phytohormones like gibberellic acid (GAᴣ), indole acetic acid (IAA), and salicylic acid (SA) take part in the biosynthesis of salt tolerance proteins under salinity. These salt tolerance proteins acclimate plants under salinity stress. Application of biotechnology plays a significant role in agriculture (Khan et al., 2017). Therefore, production of particular proteins under salt stress is a specific response of cell which can be analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE is the simple, valid, and cost-effective biochemical marker (Mushtaq et al., 2018). This marker has been widely used to determine the extent of evolutionary variations in crops (El-Kafafi et al., 2015).OBJECTIVES The present study was directed first time with the aim to investigate the toxic effect of sodium chloride (0-350 mM) and stress acclimation by pre-treatment of GAᴣ, IAA, and SA on the proteome profile of NM-92 cultivar of a Pakistani green gram.MATERIALS AND METHODSThe present study was replicated thrice in the plant laboratory of Department of Genetics, Faculty of Science, and University of Karachi. The seeds of mung bean cultivar NM-92 were acquired from National Agricultural Research Centre (NARC), Islamabad. These freshly collected 15 seedsˉ1 treatment / replication were divided into two sets. The first was named as sodium chloride (SC) stress treatments were imbibed in sterile distilled water (DW) whereas, second set soaked in gibberellic acid (GAᴣ) (BDH Chemicals, England), indole acetic acid (IAA) (Fluka, Switzerland), and salicylic acid (SA) (J.T. Baker, Holland) in the separate beaker for 24 hours under dark condition. After 24 hours, given ample time to both the sets at room temperature. After recovery, all 20 treatments were sown in the 150 X 30 mm sized petri-dishes containing 0, 50, 150, 250, and 350 millimolar (mM) sodium chloride solution (Fisher Scientific, UK) for 72 hours.Protein extraction: Protein extraction was done by taking 0.3g of seedlings in an ice chilled mortar and crushed by adding 600µL 0.2 M Tris-HCl buffer having pH 7.5 contained 5% SDS (w/v) and 5% 2-mercaptoethanol (v/v). The homogenate was incubated at 0oC for 30 min., boiled in the water bath for 3 min. at 100oC. Samples were centrifuged in Heraeus Biofuge D-37520, Germany for 30 min. at 8000 rpm. The protein supernatant was saved at below 0°C for quantitative and qualitative determination with minor modifications. The total soluble protein content of the samples was estimated via “Bovine Serum Albumin (BSA) standard curve” and explicit in µg protein milligramˉ1 fresh weight of mung seedlings.Bovine serum albumin standard curve (2000 μg/mL): Total protein standard curve was made by dissolving 0.05g of Bovine Serum Albumin (BSA) in 25mL of distilled water. Ten serial dilutions were made from 0.1 mL to 1mL by BSA solution then performed Lowry. A standard curve of total proteins was plotted by taking BSA absorbance at Y-axis and 2000 μg BSA / mL at X-axisSample preparation for SDS-PAGE: For qualitative assessment of total proteins; the 35μL of saved protein supernatant was combined with 15μL of sample diluting buffer (SDB). The SDB was made up of 0.0625 M Tris-HCl pH 6.8 with 2% of SDS, 10% of glycerol, 0.003% of bromophenol blue dye and 5% of 2-mercaptoethanol. Boil the 50μL protein SDB supernatant at 100oC in water bath for 3 min., centrifuged at 6000 rpm for 4 min. The supernatant was loaded on SDS-PAGE gel with the given formulae. The SDS- PAGE: Total proteins were fractionated via SDS-PAGE with 4% stacking and 12% resolving gel. The resolving gel of 12% was made by taking 6mL solution A, 1.8 mL 3 M Tris 1 M HCl buffer pH 8.8, 144μL 10% SDS, 5.74 mL sterile distilled water, 720μL 1.5% ammonium persulphate (APS) in deionized water and 10μL TEMED. While, stacking was composed of 1.25mL of solution A, 2.5mL of 0.5M Tris 1M HCl buffer pH 6.8, 100μL 10% SDS, 1.8 mL of distilled water, 500μL 1.5% APS and 12μL TEMED. Solution A was prepared by conjoining 30% acrylamide and 0.8% N, N’-methylene-bisacrylamide in deionized water. To avoid polymerization in the beaker; the prepared solution was quickly poured into the 3 mm thick gel plates after adding TEMED. The stacking was lined over resolving gel, then combs were inserted between the gel plates of SCIE-PLAS TV-100 separation system, UK, and allowed to polymerize for ½ an hour. After polymerization gel was placed in the tank which were filled with Tris-Glycine buffer (electrode buffer) pH 8.4 then combs were removed. The electrode buffer contained 0.3% Tris, 1.41% Glycine and 0.1% SDS in 2000mL d/w. The gel was pre-run for 15 min. at 60 volts and 120 mA currents. The prepared SDS-PAGE samples were loaded in wells with BlueStepTM Broad Range Protein Marker, AMRESCO, USA as standard and run at 60 volts & 120 mA for about 45 min. When samples entered in resolving gel, and then gave 100 volts and 200 mA currents for around 2.5 hours. Furthermore, electrophoresis was carried out at a constant watt.The Gel was washed with 30% ethanol on Uni Thermo Shaker NTS-1300 EYELA, Japan at the constant shaking for 30 min. Then gels were placed in 10% glacial acetic acid in 50% methanol solution (Fixative) for 24 hours. SDS Gel was stained until protein bands were visible thereat placed as 5% of Methanol in 7.5% acetic acid glacial solution to destain the bands background. SDS-PAGE stain composed of 0.125% coomassie brilliant blue R-250 dissolved in 40% of Methanol and 7% acetic acid glacial solution. The stain was stirred on Magnetic stirrer & hot plate M6/1, Germany for 6-10 hours before used. Photographs were taken by Sanyo digital camera VPC-T1284BL and bands were scored through numbering pattern. Gels preserved in 10% acetic acid solution at 4°C.Interpretation of bands and data analysis: The total soluble protein bands relative mobility calculated by below formulae and Dendrogram was constructed via SPSS v. 20Where,F=(Migrated distance of protein band)/(Migrated distance of dye front)Slop=(Log MW of protein marker lower limit band–log〖MW of protein marker upper limit band )/(RF protein marker lower limit band –RF of protein marker upper limit band)RESULTS:The total soluble proteins extracted from green gram were perceived by SDS-PAGE Blue StepTm broad range biochemical markers. The protein-based marker was used to evaluate the toxic effect of sodium chloride along with pre-treatments of GAᴣ, IAA, and SA on proteome assay. In the current work, seedlings total soluble proteome resolved 24 polypeptide bands ranging from 200 to 17.1 kDa were recognized by using SDS-PAGE. The figure 1 showed Dendrogram assay, which classified the 20 treatments of SC, GAᴣ, IAA and SA into two major clusters where, the cluster I was the largest one (figure 1). Cluster I consisted of 15 treatments that further divided into I-A, and I-B. The pre-treatments of SC50+SA, SC150+SA, SC250+SA, and SC350+IAA were grouped together into C-1 of sub-cluster I-A. The C-2 of sub-cluster I-A, pre-treatment SC350+SA was most diverse among 20 treatments. The C-1 treatments showed 99% homology when compared with each other while, it was 97% similar with C-2. The sub-cluster I-B comprised another 10 treatments, SC0+GAᴣ, SC50+GAᴣ, SC150+GAᴣ, SC250+GAᴣ, SC350+GAᴣ, SC0+IAA, SC50+IAA, SC150+IAA, SC250+IAA, and SC0+SA that were also 99% similar for total proteins. Sub-cluster I-B pre-treatments was exhibiting 94% homology with the sub-cluster I-A. The second cluster was the smallest one that was divided into two sub-clusters, II-A and II-B. The II-A was comprised of SC50, SC150, and SC250 while, sub-cluster II-B consisted of SC0 and SC350. Within each sub-cluster, pre-treatments expressed 99% homology whereas, II-A was 97 different from II-B. Furthermore, cluster I showed 75% similarities with cluster II (figure 1). The seedlings storage proteome profile of green gram was shown in table 1.The results showed that 120kDa, 114.6 kDa, 51.8 kDa, 29.1 kDa and 22.8 kDa proteins bands were not induced at 0 mM SC, GAᴣ, IAA, and SA. The table 1 depicted the presence of 120 kDa and 114.6 kDa bands only at 350 mM SC level with all phytohormones treatments. Similarly, 51.8 kDa protein bands were appearing at 150SC, 250SC and 350SC stress with phytohormones. Based on the information collected from the NCBI protein database, this peptide was related to the 8S globulin alpha subunits. The two other, 7S globulins sub-units having 29.1kDa and 22.8 kDa molecular weights bands were synthesized under 50mM, 150mM, 250mM, 350mM SC stress with phytohormones. Concerning protein polypeptide of molecular weight 104.5 kDa, 99.8 kDa, 91.0 kDa, 55.0 kDa, and 46.0 kDa, those were induced by GAᴣ, IAA and SA at 0 to 350 mM SC. While, 17kDa protein band was appearing in SA, and IAA treated samples and 95.3kDa band was only present in SA treatment. Other 12 protein bands were present in all treatments proved as house-keeping proteins of green gram (table 1).DISCUSSIONThe SDS-PAGE profiling for proteome is the reliable and applied biochemical approach that has been used as biochemical marker in various crop differentiation, and characterization. In the current study, first time SDS-PAGE was utilized to investigate the impact of GAᴣ, IAA, and SA pre-soaking on green gram under salt toxicity. The salt toxicity adversely affects all seed, seedling, and plant metabolic process (Parveen et al., 2016). At salt toxicity, the endogenous GAᴣ, IAA, and SA levels markedly decrease (El-Khallal et al., 2009). In such condition, exogenous application of GAᴣ, IAA, and SA enhance seedlings survival rate by increasing synthesis of seed storage proteins. Likewise, our Dendrogram characterization based on 20 treatments showed significant diversity under 0 to 350 mM SC stress. The salicylic acid treatments were grouped together except SC0+SA treatment, exhibiting a close relationship, which proved its acclimating role under salt stress. These findings will help plant breeder toward enhancing food quality and quantity of green gram in future breeding programme on saline sodic land.The SDS-PAGE assay revealed 200. kDa, 109.4 kDa, 77 kDa, 68 kDa, 49 kDa, 38 kDa, 33 kDa, 26 kDa, 24 kDa, 22 kDa, 21 kDa and 19 kDa fractions as essential green gram proteins. Among these, 68 kDa, 49 kDa, 33 kDa, 26 kDa, 24 kDa and 21 kDa peptides were seed biotinylated isoform protein (Riascos et al., 2009), putative NADH-ubiquinone oxidoreductase subunit H (Gostinčar et al., 2019), heat shock protein 33 (Hamidian et al., 2015), globulin protein, seed coat / maturation protein (Dhaubhadel et al., 2005), and protein for dimerization. While, 22 kDa proteins belonged to the class of prolamin alpha zein Z1C1_2, Z1C1_4, and Z1C1_8 precursors, and 19kDa peptide was related with Z1A1_2, Z1A2_2, and Z1B_6 precursors (Miclaus et al., 2011). Further, the 91 kDa peptide is sucrose synthase SS1 protein, and 77kDa protein is the NADPH-cytochrome P450 reductase (Wang et al., 2004). Also, the phosphatase-associated two other proteins having 46 and 55 kDa molecular weight were reported earlier in Mucuna pruriens. Hameed et al. (2012) and Malviya et al. (2008) found 55 and 46kDa peptides as 7S vicilin small sub-units and 17kDa as 11S globulins sub-unit in the studied Vigna radiata. Some other molecular weight proteome such as 68 kDa and 49kDa are 7S vicilin, 33kDa is 8S vicilin, 38 and 26kDa 8S globulins, 24kDa 11S globulins, and 22kDa 16.5S globulins. These proteins required for germination and seed establishment of green gram plant (Hameed et al., 2012).The vast accumulation of 23kDa and 22kDa peptides under salt stress by salicylic acid, were reported previously in the mangrove Bruguiera parviffora and Zea mays (El-Khallal et al., 2009). Correspondingly, El-Kafafi et al. (2015) reported the presence of 115kDa, 23kDa, and 22kDa bands in the salt tolerant lines of green gram. These proteomes induced under salt stress may play a pivotal part in the stress acclimation and osmotic adjustment. Similarly, the induction of 104 kDa and 100kDa MW polypeptide by SC stress in the salt tolerant genotypes of green gram indicated the functional role of phytohormones in various metabolic and defense response El-Kafafi et al. (2015); Alharby et al. (2016), El-Khallal et al. (2009), Qados (2010). Ali et al. (2007), Alharby et al. (2016), and El-Kafafi et al. (2015) observed 17kDa, 26kDa, 33kDa and 77kDa bands involving in salt tolerance and can be considered as a positive biochemical marker for salt stress. Further, 26 kDa MW peptide also functions as osmotin under the salt stress that involved in enhancing the accumulation of glycine betaine and proline in the cells. Hence, proteome assay of green gram showed that GAᴣ, IAA, and SA could regulate the expression of salt stress proteins that are anticipated to play a crucial part in the salt tolerance mechanism. Likewise, the involvement of phytohormones in the induction of changes in the proteome profile pattern was attributed to their part in managing cell division by regulating some genes of apical meristems.CONCLUSIONFinally, the results revealed the presence of the ten new bands with MW of 200kDa, 120 kDa, 114.6 kDa, 109.4kDa, 104.5kDa, 99.8kDa, 95.3kDa, 51.8kDa, 29.1kDa and 22.8kDa have not reported previously under salt stress with phytohormones treatments in green gram. Furthermore, it was observed that phytohormones alleviate the negative impact of salt stress on green gram by enhancing synthesis of salt defense polypeptides. Hence, higher accumulation of proteins was observed in salicylic acid treated seedlings. Thus, present work recommended the pre-soaking of phytohormones to overcome the toxic impact of sodium chloride on green gram. 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Kajian ini berfokus pada pemetaan pendekatan studi Islam dalam salah satu karya Richard C. Martin yang disunting berjudul Approaches to Islam in Religious Studies. Penelitian ini mengambil bentuk deskriptif-analitis yang dimulai dengan mengungkap latar belakang penulisan hingga evolusi sejarah studi agama. Melalui penelitian ini, beberapa kesimpulan yang dapat dikemukakan terkait dengan kecemasan akademik Martin, yang diakuinya dilatarbelakangi oleh kelemahan antara pendekatan teologis yang mempertahankan pemahaman normatif agama, dan sudut pandang sejarah agama yang menekankan pada deskripsi analitis dan membutuhkan jarak bagi para penelitinya. Sementara terkait dengan evolusi studi sejarah agama, ia menilai perkembangan studi independen setelah studi sejarah, antropologi, sosiologi, teologi dan studi timur, dan oleh karena itu, perkembangan studi tersebut cukup berpengaruh dalam cara sejarawan agama bekerja. Pengembangan lebih lanjut dianggap perlu untuk memisahkan studi agama dari disiplin lain. This study focuses on the mapping of the Islamic studies approach in one of Richard C. Martin's edited works entitled Approaches to Islam in Religious Studies. This study takes a descriptive-analytical form which begins by revealing the background of writing to the historical evolution of religious studies. Through this study, several conclusions that can be presented are related to Martin's academic anxiety, which he admits is motivated by a weakness between the theological approach which maintains a normative understanding of religions, and the history of religion point of view which emphasizes analytical descriptions and requires distance for the researchers.While related to the evolution of the study of the history of religion, he assessed the development of independent studies after historical studies, anthropology, sociology, theology and the study of the east, and therefore, developments in these studies were quite influential in the way historians of religions worked. further developments are deemed necessary to separate religious studies from other disciplines.

AbstractA diatom-based paleolimnological investigation was conducted on late Holocene sediments from a poorly buffered lake, informally named “Rock Basin Lake”, on Ellesmere Island, Arctic Canada. The fossil diatom record is unlike any other obtained thus far from high arctic regions, exhibiting dynamic assemblage shifts over the entire ∼3300 yr sedimentary record. Multiple proxies (i.e., diatoms, pH reconstructions, biogenic silica, C/N ratios, total organic carbon) appear to sensitively track rapid limnological changes, which are associated with distinct climate intervals as inferred from other regional proxy records. The highly responsive nature of the diatom assemblages in Rock Basin Lake, relative to those recorded from nearby alkaline sites, appears to be related to this lake's limited ability to buffer changes in pH. The dynamic species responses suggest that the diatoms in Rock Basin Lake are faithfully tracking climatic changes, and that low-alkalinity lakes may provide the most sensitive diatom-based paleolimnological records from high arctic regions.