Academic literature on the topic 'Other built environment and design not elsewhere classified'

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Journal articles on the topic "Other built environment and design not elsewhere classified"

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Hatami, Faizeh, and Jean-Claude Thill. "Spatiotemporal Evaluation of the Built Environment’s Impact on Commuting Duration." Sustainability 14, no. 12 (June 11, 2022): 7179. http://dx.doi.org/10.3390/su14127179.

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Upward trends in commuting duration and distance due to urban sprawl in the United States have raised concerns about the ensuing environmental, social and economic problems. Various urban planning approaches have been developed, hypothesizing that built environment variables such as density, diversity, design, distance to transit and destination accessibility contribute to reducing travel consumption. This study evaluates the impact of the built environment on commuting duration in Mecklenburg County, North Carolina, in two steps. First, the built environment is classified into four types of exurban, suburban, urban, and compact and transit-accessible development (CTAD). Second, the impact of built environment types on commuting duration is evaluated for 2000 and 2015 using spatial panel data models controlling for selection bias. Results show that CTAD areas have shorter commuting durations than other areas in 2015; however, the commuting duration in both CTAD and urban areas has increased over time. Given the multifaceted nature of urban transportation-built environment interactions and their importance for sustainable futures, this calls for further attention from urban researchers and planners to more comprehensively consider the various dimensions of this matter, with an explicit focus on the changing nature of urban environments.
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Tafahomi, Rahman, and Reihaneh Nadi. "Derivation of a Design Solution for the Conservation of a Historical Payab in the Redevelopment of Doloeei, Gonabad." International Journal of Built Environment and Sustainability 7, no. 1 (December 31, 2019): 1–9. http://dx.doi.org/10.11113/ijbes.v7.n1.407.

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This paper aims to demonstrate the conservation process of historic underground water access or Payab by proposing a multistage decision-making design in Gonabad city, northeast part of Iran. The Payab was in the center axis of a street in the proposed development of a new road which appeared to be against the new construction. The challenge was to develop the new road in the central axis without disturbing much the historic structure in the location. Hence, a request was made to keep both the new road and the historic Payab structure with a design solution. In order to tackle the design solution requested, a qualitative methodology was designed by way of site surveys, site documentation, graphical analysis and interviews. The findings of the research demonstrated contradicting opinions among the governors-laypeople, youngers-elders and males and females about the historic structure. Based on the findings, it was proposed that a protective shield, which aimed to conserve the historic structure under the weight of the new road is required, with a new entrance to be provided through an adjacent sidewalk to achieve consensus among those of diverse ideas. As a conclusion, conservation projects as demonstrated in this paper, like any other conservation projects elsewhere are faced with budget limitation, poor planning and the support it needed. As the historic structure has been part of the local built environment, the integration with other architectural elements in the context is of paramount importance to support the sustainability of the local built environment.
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Xu, Wangyue, Xiaojing Zhao, and Lan Wang. "Impact of Built Environment on Respiratory Health: An Empirical Study." Nano LIFE 08, no. 02 (June 2018): 1840001. http://dx.doi.org/10.1142/s1793984418400019.

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In many heavy industrialized countries such as China and India, the impact of air pollution on respiratory health has been headline news in recent years. Among the risk issues examined, exposure to particulate matter (PM) is cited as the prime contributing factor that causes respiratory diseases, yet it is traceable and controllable. In this paper, we report on an empirical study conducted in Shanghai, based on urban spatial determinants as independent variables to investigate its link to occurrence of lung cancer in their neighborhoods. A survey was conducted on a population of 472 lung cancer patients. After reliability and validity tests, only 156 pairs were included in this report. The questionnaire designed for this survey covers 11 outdoor and 6 indoor factors; these include the building density where they live, proximity to pollution sources, volume of traffic nearby, degree of enclosure by surrounding tall buildings, being residential or commercial with reference to their homes, proximity to parks, measured in terms of the plant type, green space per capita, accessibility to public open spaces for outdoor activities and water body; while parameters inside the house cover the age of the house, bedroom sizes, space per occupant, cooling-off time of taking up residency after renovation, humidity and dust inside the houses. Data collected were classified using random forest classification and further refined with Boruta algorithm for feature selection to identify possible correlation between risk of lung cancer to both outdoor and indoor factors of built environment. The results reveal a strong correlation between lung cancer and the environment where they live, so much so that the finding confirms our long-held belief that urban redevelopment could play an important role in reducing the risk of respiratory disease. Since prevention is better than cure, if by design to relocate pollution sources away from residential areas, provision of good public transportation to cut down vehicles on our streets, creation of green spaces to improve airflow pathway to deduce the concentration of PM in the atmosphere in our neighborhoods, we could perhaps reduce or even prevent lung cancer and a range of other respiratory diseases for the residents we served.
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Perera, Srinath, Onaopepo Adeniyi, and Solomon Olusola Babatunde. "Analysing community needs and skills for enhancing disaster resilience in the built environment." International Journal of Disaster Resilience in the Built Environment 8, no. 3 (June 12, 2017): 292–305. http://dx.doi.org/10.1108/ijdrbe-10-2015-0046.

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PurposeA better cooperation among all stakeholders working towards enhancing the disaster resilience of societies can only be achieved if the expectations or the needs of each stakeholder are understood. This study attempts to outline the needs of communities affected by disasters for the purpose of aligning the needs and skill requirements with the abilities of built environment professionals serving these communities. Therefore, the study aims to identify and describe community needs and skills requirements for enhancing disaster resilience. Design/methodology/approachThe study adopted literature review and semi-structured interviews. The semi-structured interviews were conducted with key members of some communities affected by disasters and with some of the professionals who participated in the restoration/reconstruction of those communities. Data obtained were analysed using NVivo 10. FindingsThe study revealed the current and emerging needs and skills of communities related to the built environment professionals from the viewpoint of enhancing disaster resilience. Thus, 29 classifications of skill and needs were derived and classified under five major disaster resilience dimensions to include social, economic, technological, environmental and institutional aspects. Research limitations/implicationsThis study focuses only of the needs and skills of the “community”, which is the major stakeholder that is basically the receiver of all that the other stakeholders of disaster resilience have to offer. Practical implicationsThis study would help the built environment professionals involved in disaster resilience to become aware of the specific needs and skills of the communities affected by disasters for the purpose of developing their competences. Originality/valueThe study findings would be useful for both the built environment professionals and higher education institutions. Because it is important for professionals to update and upgrade their knowledge towards enhancing their capabilities and meeting stakeholders’ expectations in a bid to enhance societal resilience to disasters across all domains of resilience.
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Shahzad, Muhammad, Muhammad Tariq Shafiq, Dean Douglas, and Mohamad Kassem. "Digital Twins in Built Environments: An Investigation of the Characteristics, Applications, and Challenges." Buildings 12, no. 2 (January 25, 2022): 120. http://dx.doi.org/10.3390/buildings12020120.

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The concept of digital twins is proposed as a new technology-led advancement to support the processes of the design, construction, and operation of built assets. Commonalities between the emerging definitions of digital twins describe them as digital or cyber environments that are bidirectionally-linked to their physical or real-life replica to enable simulation and data-centric decision making. Studies have started to investigate their role in the digitalization of asset delivery, including the management of built assets at different levels within the building and infrastructure sectors. However, questions persist regarding their actual applications and implementation challenges, including their integration with other digital technologies (i.e., building information modeling, virtual and augmented reality, Internet of Things, artificial intelligence, and cloud computing). Within the built environment context, this study seeks to analyze the definitions and characteristics of a digital twin, its interactions with other digital technologies used in built asset delivery and operation, and its applications and challenges. To achieve this aim, the research utilizes a thorough literature review and semi-structured interviews with ten industry experts. The literature review explores the merits and the relevance of digital twins relative to existing digital technologies and highlights potential applications and challenges for their implementation. The data from the semi-structured interviews are classified into five themes: definitions and enablers of digital twins, applications and benefits, implementation challenges, existing practical applications, and future development. The findings provide a point of departure for future research aimed at clarifying the relationship between digital twins and other digital technologies and their key implementation challenges.
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Kartal, Semiha, and Ömer Chousein. "Utilization of renewable energy sources in bioclimatic architecture in Greece." World Journal of Engineering 13, no. 1 (February 8, 2016): 18–22. http://dx.doi.org/10.1108/wje-02-2016-002.

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Purpose The aim of this study is to propose solutions to ensure comfort conditions for users by taking advantage of the bioclimatic architecture. This could serve as a solution to the energy shortage problem. Design/methodology/approach In this context, 40 bioclimatic buildings located in Greece were analyzed. These bioclimatic buildings were classified according to their climatic zones, functions, parameters pertaining to the physical and built environment, and the passive methods used for heating, cooling and lighting. Findings The high share of residential buildings in the distribution of Greece’s bioclimatic buildings according to climatic zones can be attributed to the large number of users that residential buildings have when compared to other types of buildings. Among the physical environmental parameters, climatic parameters were found to be highly influential. Other parameters had less significant effects, depending on land conditions. Originality value This study revealed the importance of bioclimatic applications in creating energy-efficient buildings. It is also important in terms of setting an example for creating bioclimatic architectural profiles of other countries.
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Xue, Ting, and Huiqi Liu. "The prediction of petition based on Big Data." Information Discovery and Delivery 47, no. 3 (August 19, 2019): 135–42. http://dx.doi.org/10.1108/idd-08-2018-0031.

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Purpose The development of Big Data and online searching engine provides a good opportunity for studying petition in China. This study has constructed a set of indices for predicting petitions in China by using online searching engines and further explored the predicting role of economic, environment and public life risk perception in various petitions. Design/methodology/approach Based on the study of Xue and Liu (2017), this research first re-classified offline petition by human and cluster analysis in terms of social risk perception and built online searching indices of the two sets of petition by using data from “Google Trend” and “Baidu Index.” Second, it analyzed the predicting effect of social risk perception on online searching indices of petition by using Granger causality analysis. Finally, this study integrated the results and selected significant paths from social risk perception to the two sets of petition. Findings The study found that the re-classification made by human was more appropriate than the categories made by cluster analysis in terms of social risk perception. For the two sets of petition, the correlations between offline petition and Baidu Index of petition were both more significant than that of Google index. Moreover, economic and finance and resource and environment risk perception had a significant predicting effect on more than one kind of online searching indices of petition. Originality/value The results have demonstrated the important role of economic issues in China on predicting petitions of the economic kind, as well as other kinds. They have also reflected the dominant social contradictions and their relationship in modern China.
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J. Wilkinson, Sara. "Office building adaptation and the growing significance of environmental attributes." Journal of Corporate Real Estate 16, no. 4 (November 4, 2014): 252–65. http://dx.doi.org/10.1108/jcre-06-2014-0014.

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Purpose – The purpose of this study was to investigated the importance of environmental attributes for office building adaptation and whether the importance of environmental attributes for adaptation has changed over time from 1998-2008 to 2009-2011. With 1-2 per cent added to the total stock of buildings each year and the need to take action to mitigate the impacts of predicted climate change (IPCC, 2013), it is necessary to focus efforts on adaptation of existing buildings. Design/methodology/approach – This research adopted a quantitative approach, using a database of office building attributes and applying principal component analysis to ascertain the respective importance of various building attributes in adaptation. Using two databases; the first dating from 1998 to 2008 and comprising 5,290 adaptation events and the second covering the period 2009 to 2011 and comprising 1,272 adaptation events, a comparison of results was undertaken. Findings – The findings indicate the importance of some environmental attributes in building adaptation has changed and that legislation and changes market perceptions towards to promote built environment sustainability may be having a positive impact. The research demonstrates that different property attributes vary in importance over time and used existing buildings in an international city to confirm application to urban settlements elsewhere where existing buildings can be adapted to reduce the effect of climate change. Research limitations/implications – The databases are limited to Melbourne, Australia and to these specific points in time. It is possible that other cities are seeing changes in adaptation practices to accommodate increased awareness and the growing importance attributed to environmental issues; however, additional studies would be required to ascertain whether the level of importance was stronger or weaker than that found in Melbourne. Practical implications – The impacts of the mandatory The National Australian Built Environment Rating System energy rating tool and the Green Star voluntary tool provide actionable data for property stakeholders and the academic community. Policy-makers can see that building owners are integrating environmental attributes into their stock and that the market is shifting towards increased sustainability. This study uses real world data to feed the scholarship process, with real economic and commercial impacts. New buildings account for about 1-2 per cent of the total building stock annually and existing buildings must be adapted, and thus the questions of the success of voluntary or mandatory measures are essential to future environmental decision-making. Originality/value – This research reports on data covering all office building adaptation conducted from 1998 to 2011 in the Melbourne CBD. As such, it is a comprehensive analysis of all works undertaken and how the significance of different physical, social, economic and environmental attributes is changing over time.
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Urenev, Valeryi, and David Pishchev. "TEC TYPOLOGY USING PENTATONIC SEALS." Architectural Bulletin of KNUCA, no. 22-23 (December 12, 2021): 210–19. http://dx.doi.org/10.32347/2519-8661.2021.22-23.210-219.

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Thematic entertainment complexes are an extremely wide field in which various projects are built. From religious buildings and museums to dinosaur parks and thematic complexes dedicated to cinema. At the moment, TEC do not have a typology. They cannot be classified and systematized. The breadth of topics, sizes, target audiences and placement options is so great that one letter can not describe it. Therefore, it was decided to develop such a system. This will allow us to compare, at first glance, not comparative things, but in conjunction with the final table of the article "Analysis of psychological preconditions for the formation of TEC using the Maslow Pyramid" [2], we can analyze, evaluate, rate and compare different thematic entertainment complexes. The purpose of the study is to analyze the existing methods of typology of thematic entertainment complexes. Identify those items by which you can still classify thematic entertainment complexes. Develop a graphic display for each item and assemble it into a single, compact scheme for easy reading and placement in the design of the architectural environment. Then let's analyze the real thematic entertainment complex. For example, take Disneyland Paris, as the brightest representative of this type of building. The scientific novelty of the study is that we will form a typological system that will allow in a compact version to encrypt all the features of the thematic entertainment complex. Its size, location, topic, purpose, target audience, etc. As a result, we get a method of analysis of thematic entertainment complexes, which will allow you to quickly analyze thematic entertainment complexes, compare them with each other and more clearly structure existing and future complexes. It will also help the end visitor to immediately understand exactly where he spent and what to expect from the institution.
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Nesrine, Lenchi, Kebbouche Salima, Khelfaoui Mohamed Lamine, Laddada Belaid, BKhemili Souad, Gana Mohamed Lamine, Akmoussi Sihem, and Ferioune Imène. "Phylogenetic characterization and screening of halophilic bacteria from Algerian salt lake for the production of biosurfactant and enzymes." World Journal of Biology and Biotechnology 5, no. 2 (August 15, 2020): 1. http://dx.doi.org/10.33865/wjb.005.02.0294.

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Environments containing significant concentration of NaCl such as salt lakes harbor extremophiles microorganisms which have a great biotechnology interest. To explore the diversity of Bacteria in Chott Tinsilt (Algeria), an isolation program was performed. Water samples were collected from the saltern during the pre-salt harvesting phase. This Chott is high in salt (22.47% (w/v). Seven halophiles Bacteria were selected for further characterization. The isolated strains were able to grow optimally in media with 10–25% (w/v) total salts. Molecular identification of the isolates was performed by sequencing the 16S rRNA gene. It showed that these cultured isolates included members belonging to the Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus genera with less than 98% of similarity with their closest phylogenetic relative. The halophilic bacterial isolates were also characterized for the production of biosurfactant and industrially important enzymes. Most isolates produced hydrolases and biosurfactants at high salt concentration. In fact, this is the first report on bacterial strains (A4 and B4) which were a good biosurfactant and coagulase producer at 20% and 25% ((w/v)) NaCl. In addition, the biosurfactant produced by the strain B4 at high salinity (25%) was also stable at high temperature (30-100°C) and high alkalinity (pH 11).Key word: Salt Lake, Bacteria, biosurfactant, Chott, halophiles, hydrolases, 16S rRNAINTRODUCTIONSaline lakes cover approximately 10% of the Earth’s surface area. The microbial populations of many hypersaline environments have already been studied in different geographical regions such as Great Salt Lake (USA), Dead Sea (Israel), Wadi Natrun Lake (Egypt), Lake Magadi (Kenya), Soda Lake (Antarctica) and Big Soda Lake and Mono Lake (California). Hypersaline regions differ from each other in terms of geographical location, salt concentration and chemical composition, which determine the nature of inhabitant microorganisms (Gupta et al., 2015). Then low taxonomic diversity is common to all these saline environments (Oren et al., 1993). Halophiles are found in nearly all major microbial clades, including prokaryotic (Bacteria and Archaea) and eukaryotic forms (DasSarma and Arora, 2001). They are classified as slight halophiles when they grow optimally at 0.2–0.85 M (2–5%) NaCl, as moderate halophiles when they grow at 0.85–3.4 M (5–20%) NaCl, and as extreme halophiles when they grow at 3.4–5.1 M (20–30%) NaCl. Hyper saline environments are inhabited by extremely halophilic and halotolerant microorganisms such as Halobacillus sp, Halobacterium sp., Haloarcula sp., Salinibacter ruber , Haloferax sp and Bacillus spp. (Solomon and Viswalingam, 2013). There is a tremendous demand for halophilic bacteria due to their biotechnological importance as sources of halophilic enzymes. Enzymes derived from halophiles are endowed with unique structural features and catalytic power to sustain the metabolic and physiological processes under high salt conditions. Some of these enzymes have been reported to be active and stable under more than one extreme condition (Karan and Khare, 2010). Applications are being considered in a range of industries such as food processing, washing, biosynthetic processes and environmental bioremediation. Halophilic proteases are widely used in the detergent and food industries (DasSarma and Arora, 2001). However, esterases and lipases have also been useful in laundry detergents for the removal of oil stains and are widely used as biocatalysts because of their ability to produce pure compounds. Likewise, amylases are used industrially in the first step of the production of high fructose corn syrup (hydrolysis of corn starch). They are also used in the textile industry in the de-sizing process and added to laundry detergents. Furthermore, for the environmental applications, the use of halophiles for bioremediation and biodegradation of various materials from industrial effluents to soil contaminants and accidental spills are being widely explored. In addition to enzymes, halophilic / halotolerants microorganisms living in saline environments, offer another potential applications in various fields of biotechnology like the production of biosurfactant. Biosurfactants are amphiphilic compounds synthesized from plants and microorganisms. They reduce surface tension and interfacial tension between individual molecules at the surface and interface respectively (Akbari et al., 2018). Comparing to the chemical surfactant, biosurfactant are promising alternative molecules due to their low toxicity, high biodegradability, environmental capability, mild production conditions, lower critical micelle concentration, higher selectivity, availability of resources and ability to function in wide ranges of pH, temperature and salinity (Rocha et al., 1992). They are used in various industries which include pharmaceuticals, petroleum, food, detergents, cosmetics, paints, paper products and water treatment (Akbari et al., 2018). The search for biosurfactants in extremophiles is particularly promising since these biomolecules can adapt and be stable in the harsh environments in which they are to be applied in biotechnology.OBJECTIVESEastern Algeria features numerous ecosystems including hypersaline environments, which are an important source of salt for food. The microbial diversity in Chott Tinsilt, a shallow Salt Lake with more than 200g/L salt concentration and a superficies of 2.154 Ha, has never yet been studied. The purpose of this research was to chemically analyse water samples collected from the Chott, isolate novel extremely or moderate halophilic Bacteria, and examine their phenotypic and phylogenetic characteristics with a view to screening for biosurfactants and enzymes of industrial interest.MATERIALS AND METHODSStudy area: The area is at 5 km of the Commune of Souk-Naâmane and 17 km in the South of the town of Aïn-Melila. This area skirts the trunk road 3 serving Constantine and Batna and the railway Constantine-Biskra. It is part the administrative jurisdiction of the Wilaya of Oum El Bouaghi. The Chott belongs to the wetlands of the High Plains of Constantine with a depth varying rather regularly without never exceeding 0.5 meter. Its length extends on 4 km with a width of 2.5 km (figure 1).Water samples and physico-chemical analysis: In February 2013, water samples were collected from various places at the Chott Tinsilt using Global Positioning System (GPS) coordinates of 35°53’14” N lat. and 06°28’44”E long. Samples were collected randomly in sterile polythene bags and transported immediately to the laboratory for isolation of halophilic microorganisms. All samples were treated within 24 h after collection. Temperature, pH and salinity were measured in situ using a multi-parameter probe (Hanna Instruments, Smithfield, RI, USA). The analytical methods used in this study to measure ions concentration (Ca2+, Mg2+, Fe2+, Na+, K+, Cl−, HCO3−, SO42−) were based on 4500-S-2 F standard methods described elsewhere (Association et al., 1920).Isolation of halophilic bacteria from water sample: The media (M1) used in the present study contain (g/L): 2.0 g of KCl, 100.0/200.0 g of NaCl, 1.0 g of MgSO4.7HO2, 3.0 g of Sodium Citrate, 0.36 g of MnCl2, 10.0 g of yeast extract and 15.0 g agar. The pH was adjusted to 8.0. Different dilutions of water samples were added to the above medium and incubated at 30°C during 2–7 days or more depending on growth. Appearance and growth of halophilic bacteria were monitored regularly. The growth was diluted 10 times and plated on complete medium agar (g/L): glucose 10.0; peptone 5.0; yeast extract 5.0; KH2PO4 5.0; agar 30.0; and NaCl 100.0/200.0. Resultant colonies were purified by repeated streaking on complete media agar. The pure cultures were preserved in 20% glycerol vials and stored at −80°C for long-term preservation.Biochemical characterisation of halophilic bacterial isolates: Bacterial isolates were studied for Gram’s reaction, cell morphology and pigmentation. Enzymatic assays (catalase, oxidase, nitrate reductase and urease), and assays for fermentation of lactose and mannitol were done as described by Smibert (1994).Optimization of growth conditions: Temperature, pH, and salt concentration were optimized for the growth of halophilic bacterial isolates. These growth parameters were studied quantitatively by growing the bacterial isolates in M1 medium with shaking at 200 rpm and measuring the cell density at 600 nm after 8 days of incubation. To study the effect of NaCl on the growth, bacterial isolates were inoculated on M1 medium supplemented with different concentration of NaCl: 1%-35% (w/v). The effect of pH on the growth of halophilic bacterial strains was studied by inoculating isolates on above described growth media containing NaCl and adjusted to acidic pH of 5 and 6 by using 1N HCl and alkaline pH of 8, 9, 10, 11 and 12 using 5N NaOH. The effect of temperature was studied by culturing the bacterial isolates in M1 medium at different temperatures of incubation (4°C–55°C).Screening of halophilic bacteria for hydrolytic enzymes: Hydrolase producing bacteria among the isolates were screened by plate assay on starch, tributyrin, gelatin and DNA agar plates respectively for amylase, lipase, protease and DNAse activities. Amylolytic activity of the cultures was screened on starch nutrient agar plates containing g/L: starch 10.0; peptone 5.0; yeast extract 3.0; agar 30.0; NaCl 100.0/250.0. The pH was 7.0. After incubation at 30 ºC for 7 days, the zone of clearance was determined by flooding the plates with iodine solution. The potential amylase producers were selected based on ratio of zone of clearance diameter to colony diameter. Lipase activity of the cultures was screened on tributyrin nutrient agar plates containing 1% (v/v) of tributyrin. Isolates that showed clear zones of tributyrin hydrolysis were identified as lipase producing bacteria. Proteolytic activity of the isolates was similarly screened on gelatin nutrient agar plates containing 10.0 g/L of gelatin. The isolates showing zones of gelatin clearance upon treatment with acidic mercuric chloride were selected and designated as protease producing bacteria. The presence of DNAse activity on plates was determined on DNAse test agar (BBL) containing 10%-25% (w/v) total salt. After incubation for 7days, the plates were flooded with 1N HCl solution. Clear halos around the colonies indicated DNAse activity (Jeffries et al., 1957).Milk clotting activity (coagulase activity) of the isolates was also determined following the procedure described (Berridge, 1952). Skim milk powder was reconstituted in 10 mM aqueous CaCl2 (pH 6.5) to a final concentration of 0.12 kg/L. Enzyme extracts were added at a rate of 0.1 mL per mL of milk. The coagulation point was determined by manual rotating of the test tube periodically, at short time intervals, and checking for visible clot formation.Screening of halophilic bacteria for biosurfactant production. Oil spread Assay: The Petridis base was filled with 50 mL of distilled water. On the water surface, 20μL of diesel and 10μl of culture were added respectively. The culture was introduced at different spots on the diesel, which is coated on the water surface. The occurrence of a clear zone was an indicator of positive result (Morikawa et al., 2000). The diameter of the oil expelling circles was measured by slide caliber (with a degree of accuracy of 0.02 mm).Surface tension and emulsification index (E24): Isolates were cultivated at 30 °C for 7 days on the enrichment medium containing 10-25% NaCl and diesel oil as the sole carbon source. The medium was centrifuged (7000 rpm for 20 min) and the surface tension of the cell-free culture broth was measured with a TS90000 surface tensiometer (Nima, Coventry, England) as a qualitative indicator of biosurfactant production. The culture broth was collected with a Pasteur pipette to remove the non-emulsified hydrocarbons. The emulsifying capacity was evaluated by an emulsification index (E24). The E24 of culture samples was determined by adding 2 mL of diesel oil to the same amount of culture, mixed for 2 min with a vortex, and allowed to stand for 24 h. E24 index is defined as the percentage of height of emulsified layer (mm) divided by the total height of the liquid column (mm).Biosurfactant stability studies : After growth on diesel oil as sole source of carbone, cultures supernatant obtained after centrifugation at 6,000 rpm for 15 min were considered as the source of crude biosurfactant. Its stability was determined by subjecting the culture supernatant to various temperature ranges (30, 40, 50, 60, 70, 80 and 100 °C) for 30 min then cooled to room temperature. Similarly, the effect of different pH (2–11) on the activity of the biosurfactant was tested. The activity of the biosurfactant was investigated by measuring the emulsification index (El-Sersy, 2012).Molecular identification of potential strains. DNA extraction and PCR amplification of 16S rDNA: Total cellular DNA was extracted from strains and purified as described by Sambrook et al. (1989). DNA was purified using Geneclean® Turbo (Q-BIO gene, Carlsbad, CA, USA) before use as a template in polymerase chain reaction (PCR) amplification. For the 16S rDNA gene sequence, the purified DNA was amplified using a universal primer set, forward primer (27f; 5′-AGA GTT TGA TCM TGG CTC AG) and a reverse primer (1492r; 5′-TAC GGY TAC CTT GTT ACG ACT T) (Lane, 1991). Agarose gel electrophoresis confirmed the amplification product as a 1400-bp DNA fragment.16S rDNA sequencing and Phylogenic analysis: Amplicons generated using primer pair 27f-1492r was sequenced using an automatic sequencer system at Macrogene Company (Seoul, Korea). The sequences were compared with those of the NCBI BLAST GenBank nucleotide sequence databases. Phylogenetic trees were constructed by the neighbor-joining method using MEGA version 5.05 software (Tamura et al., 2011). Bootstrap resembling analysis for 1,000 replicates was performed to estimate the confidence of tree topologies.Nucleotide sequence accession numbers: The nucleotide sequences reported in this work have been deposited in the EMBL Nucleotide Sequence Database. The accession numbers are represented in table 5.Statistics: All experiments were conducted in triplicates. Results were evaluated for statistical significance using ANOVA.RESULTSPhysico-chemical parameters of the collected water samples: The physicochemical properties of the collected water samples are reported in table 1. At the time of sampling, the temperature was 10.6°C and pH 7.89. The salinity of the sample, as determined in situ, was 224.70 g/L (22,47% (w/v)). Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions (table 1). SO4-2 and Mg+2 was present in much smaller amounts compared to Na +and Cl- concentration. Low levels of calcium, potassium and bicarbonate were also detected, often at less than 1 g/L.Characterization of isolates. Morphological and biochemical characteristic feature of halophilic bacterial isolates: Among 52 strains isolated from water of Chott Tinsilt, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for further characterization (table 2). The colour of the isolates varied from beige, pale yellow, yellowish and orange. The bacterial isolates A1, A2, A4, B1 and B5 were rod shaped and gram negative (except B5), whereas A3 and B4 were cocci and gram positive. All strains were oxidase and catalase positive except for B1. Nitrate reductase and urease activities were observed in all the bacterial isolates, except B4. All the bacterial isolates were negative for H2S formation. B5 was the only strain positive for mannitol fermentation (table 2).We isolated halophilic bacteria on growth medium with NaCl supplementation at pH 7 and temperature of 30°C. We studied the effect of NaCl, temperature and pH on the growth of bacterial isolates. All the isolates exhibited growth only in the presence of NaCl indicating that these strains are halophilic. The optimum growth of isolates A3 and B1 was observed in the presence of 10% NaCl, whereas it was 15% NaCl for A1, A2 and B5. A4 and B4 showed optimum growth in the presence of 20% and 25% NaCl respectively. A4, B4 and B5 strains can tolerate up to 35% NaCl.The isolate B1 showed growth in medium supplemented with 10% NaCl and pH range of 7–10. The optimum pH for the growth B1 was 9 and they did not show any detectable growth at or below pH 6 (table 2), which indicates the alkaliphilic nature of B1 isolate. The bacterial isolates A1, A2 and A4 exhibited growth in the range of pH 6–10, while A3 and B4 did not show any growth at pH greater than 8. The optimum pH for growth of all strains (except B1) was pH 7.0 (table 2). These results indicate that A1, A2, A3, A4, B4 and B5 are neutrophilic in nature. All the bacterial isolates exhibited optimal growth at 30°C and no detectable growth at 55°C. Also, detectable growth of isolates A1, A2 and A4 was observed at 4°C. However, none of the bacterial strains could grow below 4°C and above 50°C (table 2).Screening of the halophilic enzymes: To characterize the diversity of halophiles able to produce hydrolytic enzymes among the population of microorganisms inhabiting the hypersaline habitats of East Algeria (Chott Tinsilt), a screening was performed. As described in Materials and Methods, samples were plated on solid media containing 10%-25% (w/v) of total salts and different substrates for the detection of amylase, protease, lipase and DNAse activities. However, coagulase activity was determined in liquid medium using milk as substrate (figure 3). Distributions of hydrolytic activity among the isolates are summarized in table 4.From the seven bacterial isolates, four strains A1, A2, A4 and B5 showed combined hydrolytic activities. They were positive for gelatinase, lipase and coagulase. A3 strain showed gelatinase and lipase activities. DNAse activities were detected with A1, A4, B1 and B5 isolates. B4 presented lipase and coagulase activity. Surprisingly, no amylase activity was detected among all the isolates.Screening for biosurfactant producing isolates: Oil spread assay: The results showed that all the strains could produce notable (>4 cm diameter) oil expelling circles (ranging from 4.11 cm to 4.67 cm). The average diameter for strain B5 was 4.67 cm, significantly (P < 0.05) higher than for the other strains.Surface tension and emulsification index (E24): The assimilation of hydrocarbons as the sole sources of carbon by the isolate strains led to the production of biosurfactants indicated by the emulsification index and the lowering of the surface tension of cell-free supernatant. Based on rapid growth on media containing diesel oil as sole carbon source, the seven isolates were tested for biosurfactant production and emulsification activity. The obtained values of the surface tension measurements as well as the emulsification index (E24) are shown in table 3. The highest reduction of surface tension was achieved with B5 and A3 isolates with values of 25.3 mN m−1 and 28.1 mN m−1 respectively. The emulsifying capacity evaluated by the E24 emulsification index was highest in the culture of isolate B4 (78%), B5 (77%) and A3 (76%) as shown in table 3 and figure 2. These emulsions were stable even after 4 months. The bacteria with emulsification indices higher than 50 % and/or reduction in the surface tension (under 30 mN/m) have been defined as potential biosurfactant producers. Based on surface tension and the E24 index results, isolates B5, B4, A3 and A4 are the best candidates for biosurfactant production. It is important to note that, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was choosen for futher analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4.biosurfactant in medium containing respectively 25% and 20% (w/v) NaCl.Stability of biosurfactant activities: The applicability of biosurfactants in several biotechnological fields depends on their stability at different environmental conditions (temperatures, pH and NaCl). For this study, the strain B4 appear very interesting (It can produce biosurfactant at 25 % NaCl) and was chosen for further analysis for biosurfactant stability. The effects of temperature and pH on the biosurfactant production by the strain B4 are shown in figure 4. The biosurfactant produced by this strain was shown to be thermostable giving an E-24 Index value greater than 78% (figure 4A). Heating of the biosurfactant to 100 °C caused no significant effect on the biosurfactant performance. Therefore, the surface activity of the crude biosurfactant supernatant remained relatively stable to pH changes between pH 6 and 11. At pH 11, the value of E24 showed almost 76% activity, whereas below pH 6 the activity was decreased up to 40% (figure 4A). The decreases of the emulsification activity by decreasing the pH value from basic to an acidic region; may be due to partial precipitation of the biosurfactant. This result indicated that biosurfactant produced by strain B4 show higher stability at alkaline than in acidic conditions.Molecular identification and phylogenies of potential isolates: To identify halophilic bacterial isolates, the 16S rDNA gene was amplified using gene-specific primers. A PCR product of ≈ 1.3 kb was detected in all the seven isolates. The 16S rDNA amplicons of each bacterial isolate was sequenced on both strands using 27F and 1492R primers. The complete nucleotide sequence of 1336,1374, 1377,1313, 1305,1308 and 1273 bp sequences were obtained from A1, A2, A3, A4, B1, B4 and B5 isolates respectively, and subjected to BLAST analysis. The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus as shown in table 5. The halophilic isolates A2 and A4 showed 97% similarity with the Halomonas variabilis strain GSP3 (accession no. AY505527) and the Halomonas sp. M59 (accession no. AM229319), respectively. As for A1, it showed 96% similarity with the Halomonas venusta strain GSP24 (accession no. AY553074). B1 and B4 showed for their part 96% similarity with the Salinivibrio costicola subsp. alcaliphilus strain 18AG DSM4743 (accession no. NR_042255) and the Planococcus citreus (accession no. JX122551), respectively. The bacterial isolate B5 showed 98% sequence similarity with the Halobacillus trueperi (accession no. HG931926), As for A3, it showed only 95% similarity with the Staphylococcus arlettae (accession no. KR047785). The 16S rDNA nucleotide sequences of all the seven halophilic bacterial strains have been submitted to the NCBI GenBank database under the accession number presented in table 5. The phylogenetic association of the isolates is shown in figure 5.DICUSSIONThe physicochemical properties of the collected water samples indicated that this water was relatively neutral (pH 7.89) similar to the Dead Sea and the Great Salt Lake (USA) and in contrast to the more basic lakes such as Lake Wadi Natrun (Egypt) (pH 11) and El Golea Salt Lake (Algeria) (pH 9). The salinity of the sample was 224.70 g/L (22,47% (w/v). This range of salinity (20-30%) for Chott Tinsilt is comparable to a number of well characterized hypersaline ecosystems including both natural and man-made habitats, such as the Great Salt Lake (USA) and solar salterns of Puerto Rico. Thus, Chott Tinsilt is a hypersaline environment, i.e. environments with salt concentrations well above that of seawater. Chemical analysis of water sample indicated that Na +and Cl- were the most abundant ions, as in most hypersaline ecosystems (with some exceptions such as the Dead Sea). These chemical water characteristics were consistent with the previously reported data in other hypersaline ecosystems (DasSarma and Arora, 2001; Oren, 2002; Hacěne et al., 2004). Among 52 strains isolated from this Chott, seven distinct bacteria (A1, A2, A3, A4, B1, B4 and B5) were chosen for phenotypique, genotypique and phylogenetique characterization.The 16S rDNA sequence analysis showed that the isolated strains belong to the genera Halomonas, Staphylococcus, Salinivibrio, Planococcus and Halobacillus. Genera obtained in the present study are commonly occurring in various saline habitats across the globe. Staphylococci have the ability to grow in a wide range of salt concentrations (Graham and Wilkinson, 1992; Morikawa et al., 2009; Roohi et al., 2014). For example, in Pakistan, Staphylococcus strains were isolated from various salt samples during the study conducted by Roohi et al. (2014) and these results agreed with previous reports. Halomonas, halophilic and/or halotolerant Gram-negative bacteria are typically found in saline environments (Kim et al., 2013). The presence of Planococcus and Halobacillus has been reported in studies about hypersaline lakes; like La Sal del Rey (USA) (Phillips et al., 2012) and Great Salt Lake (Spring et al., 1996), respectively. The Salinivibrio costicola was a representative model for studies on osmoregulatory and other physiological mechanisms of moderately halophilic bacteria (Oren, 2006).However, it is interesting to note that all strains shared less than 98.7% identity (the usual species cut-off proposed by Yarza et al. (2014) with their closest phylogenetic relative, suggesting that they could be considered as new species. Phenotypic, genetic and phylogenetic analyses have been suggested for the complete identification of these strains. Theses bacterial strains were tested for the production of industrially important enzymes (Amylase, protease, lipase, DNAse and coagulase). These isolates are good candidates as sources of novel enzymes with biotechnological potential as they can be used in different industrial processes at high salt concentration (up to 25% NaCl for B4). Prominent amylase, lipase, protease and DNAase activities have been reported from different hypersaline environments across the globe; e.g., Spain (Sánchez‐Porro et al., 2003), Iran (Rohban et al., 2009), Tunisia (Baati et al., 2010) and India (Gupta et al., 2016). However, to the best of our knowledge, the coagulase activity has never been detected in extreme halophilic bacteria. Isolation and characterization of crude enzymes (especially coagulase) to investigate their properties and stability are in progress.The finding of novel enzymes with optimal activities at various ranges of salt concentrations is of great importance. Besides being intrinsically stable and active at high salt concentrations, halophilic and halotolerant enzymes offer great opportunities in biotechnological applications, such as environmental bioremediation (marine, oilfiel) and food processing. The bacterial isolates were also characterized for production of biosurfactants by oil-spread assay, measurement of surface tension and emulsification index (E24). There are few reports on biosurfactant producers in hypersaline environments and in recent years, there has been a greater increase in interest and importance in halophilic bacteria for biomolecules (Donio et al., 2013; Sarafin et al., 2014). Halophiles, which have a unique lipid composition, may have an important role to play as surface-active agents. The archae bacterial ether-linked phytanyl membrane lipid of the extremely halophilic bacteria has been shown to have surfactant properties (Post and Collins, 1982). Yakimov et al. (1995) reported the production of biosurfactant by a halotolerant Bacillus licheniformis strain BAS 50 which was able to produce a lipopeptide surfactant when cultured at salinities up to 13% NaCl. From solar salt, Halomonas sp. BS4 and Kocuria marina BS-15 were found to be able to produce biosurfactant when cultured at salinities of 8% and 10% NaCl respectively (Donio et al., 2013; Sarafin et al., 2014). In the present work, strains B4 and A4 produce biosurfactant in medium containing respectively 25% and 20% NaCl. To our knowledge, this is the first report on biosurfactant production by bacteria under such salt concentration. Biosurfactants have a wide variety of industrial and environmental applications (Akbari et al., 2018) but their applicability depends on their stability at different environmental conditions. The strain B4 which can produce biosurfactant at 25% NaCl showed good stability in alkaline pH and at a temperature range of 30°C-100°C. Due to the enormous utilization of biosurfactant in detergent manufacture the choice of alkaline biosurfactant is researched (Elazzazy et al., 2015). On the other hand, the interesting finding was the thermostability of the produced biosurfactant even after heat treatment (100°C for 30 min) which suggests the use of this biosurfactant in industries where heating is of a paramount importance (Khopade et al., 2012). To date, more attention has been focused on biosurfactant producing bacteria under extreme conditions for industrial and commercial usefulness. In fact, the biosurfactant produce by strain B4 have promising usefulness in pharmaceutical, cosmetics and food industries and for bioremediation in marine environment and Microbial enhanced oil recovery (MEOR) where the salinity, temperature and pH are high.CONCLUSIONThis is the first study on the culturable halophilic bacteria community inhabiting Chott Tinsilt in Eastern Algeria. Different genera of halotolerant bacteria with different phylogeneticaly characteristics have been isolated from this Chott. Culturing of bacteria and their molecular analysis provides an opportunity to have a wide range of cultured microorganisms from extreme habitats like hypersaline environments. Enzymes produced by halophilic bacteria show interesting properties like their ability to remain functional in extreme conditions, such as high temperatures, wide range of pH, and high salt concentrations. These enzymes have great economical potential in industrial, agricultural, chemical, pharmaceutical, and biotechnological applications. Thus, the halophiles isolated from Chott Tinsilt offer an important potential for application in microbial and enzyme biotechnology. In addition, these halo bacterial biosurfactants producers isolated from this Chott will help to develop more valuable eco-friendly products to the pharmacological and food industries and will be usefulness for bioremediation in marine environment and petroleum industry.ACKNOWLEDGMENTSOur thanks to Professor Abdelhamid Zoubir for proofreading the English composition of the present paper.CONFLICT OF INTERESTThe authors declare that they have no conflict of interest.Akbari, S., N. H. Abdurahman, R. M. Yunus, F. Fayaz and O. R. Alara, 2018. Biosurfactants—a new frontier for social and environmental safety: A mini review. Biotechnology research innovation, 2(1): 81-90.Association, A. P. H., A. W. W. Association, W. P. C. Federation and W. E. Federation, 1920. Standard methods for the examination of water and wastewater. American Public Health Association.Baati, H., R. Amdouni, N. Gharsallah, A. Sghir and E. Ammar, 2010. Isolation and characterization of moderately halophilic bacteria from tunisian solar saltern. Current microbiology, 60(3): 157-161.Berridge, N., 1952. Some observations on the determination of the activity of rennet. Analyst, 77(911): 57b-62.DasSarma, S. and P. Arora, 2001. Halophiles. Encyclopedia of life sciences. Nature publishishing group: 1-9.Donio, M. B. S., F. A. Ronica, V. T. Viji, S. Velmurugan, J. S. C. A. Jenifer, M. Michaelbabu, P. Dhar and T. Citarasu, 2013. Halomonas sp. Bs4, a biosurfactant producing halophilic bacterium isolated from solar salt works in India and their biomedical importance. SpringerPlus, 2(1): 149.El-Sersy, N. A., 2012. Plackett-burman design to optimize biosurfactant production by marine Bacillus subtilis n10. Roman biotechnol lett, 17(2): 7049-7064.Elazzazy, A. M., T. Abdelmoneim and O. Almaghrabi, 2015. Isolation and characterization of biosurfactant production under extreme environmental conditions by alkali-halo-thermophilic bacteria from Saudi Arabia. Saudi journal of biological Sciences, 22(4): 466-475.Graham, J. E. and B. Wilkinson, 1992. Staphylococcus aureus osmoregulation: Roles for choline, glycine betaine, proline, and taurine. Journal of bacteriology, 174(8): 2711-2716.Gupta, S., P. Sharma, K. Dev and A. Sourirajan, 2016. Halophilic bacteria of lunsu produce an array of industrially important enzymes with salt tolerant activity. Biochemistry research international, 1: 1-10.Gupta, S., P. Sharma, K. Dev, M. Srivastava and A. Sourirajan, 2015. A diverse group of halophilic bacteria exist in lunsu, a natural salt water body of Himachal Pradesh, India. SpringerPlus 4(1): 274.Hacěne, H., F. Rafa, N. Chebhouni, S. Boutaiba, T. Bhatnagar, J. C. Baratti and B. Ollivier, 2004. Biodiversity of prokaryotic microflora in el golea salt lake, Algerian Sahara. Journal of arid environments, 58(3): 273-284.Jeffries, C. D., D. F. Holtman and D. G. Guse, 1957. Rapid method for determining the activity of microorgan-isms on nucleic acids. Journal of bacteriology, 73(4): 590.Karan, R. and S. Khare, 2010. Purification and characterization of a solvent‐stable protease from Geomicrobium sp. Emb2. Environmental technology, 31(10): 1061-1072.Khopade, A., R. Biao, X. Liu, K. Mahadik, L. Zhang and C. Kokare, 2012. Production and stability studies of the biosurfactant isolated from marine Nocardiopsis sp. B4. Desalination, 3: 198-204.Kim, K. K., J.-S. Lee and D. A. Stevens, 2013. Microbiology and epidemiology of Halomonas species. Future microbiology, 8(12): 1559-1573.Lane, D., 1991. 16s/23s rRNA sequencing in nucleic acid techniques in bacterial systematics. Stackebrandt e., editor;, and goodfellow m., editor. Chichester, UK: John Wiley & Sons.Morikawa, K., R. L. Ohniwa, T. Ohta, Y. Tanaka, K. Takeyasu and T. Msadek, 2009. Adaptation beyond the stress response: Cell structure dynamics and population heterogeneity in Staphylococcus aureus. Microbes environments, 25: 75-82.Morikawa, M., Y. Hirata and T. J. B. e. B. A.-M. Imanaka, 2000. A study on the structure–function relationship of lipopeptide biosurfactants. Biochimica et biophysica acta, 1488(3): 211-218.Oren, A., 2002. Diversity of halophilic microorganisms: Environments, phylogeny, physiology, and applications. Journal of industrial microbiology biotechnology, 28(1): 56-63.Oren, A., 2006. Halophilic microorganisms and their environments. Springer science & business media.Oren, A., R. Vreeland and L. Hochstein, 1993. Ecology of extremely halophilic microorganisms. The biology of halophilic bacteria, 2(1): 1-8.Phillips, K., F. Zaidan, O. R. Elizondo and K. L. Lowe, 2012. Phenotypic characterization and 16s rDNA identification of culturable non-obligate halophilic bacterial communities from a hypersaline lake, la sal del rey, in extreme south texas (USA). Aquatic biosystems, 8(1): 1-5.Post, F. and N. Collins, 1982. A preliminary investigation of the membrane lipid of Halobacterium halobium as a food additive 1. Journal of food biochemistry, 6(1): 25-38.Rocha, C., F. San-Blas, G. San-Blas and L. Vierma, 1992. Biosurfactant production by two isolates of Pseudomonas aeruginosa. World Journal of microbiology biotechnology, 8(2): 125-128.Rohban, R., M. A. Amoozegar and A. Ventosa, 2009. Screening and isolation of halophilic bacteria producing extracellular hydrolyses from howz soltan lake, Iran. Journal of industrial microbiology biotechnology, 36(3): 333-340.Roohi, A., I. Ahmed, N. Khalid, M. Iqbal and M. Jamil, 2014. Isolation and phylogenetic identification of halotolerant/halophilic bacteria from the salt mines of Karak, Pakistan. International journal of agricultural and biology, 16: 564-570.Sambrook, J., E. F. Fritsch and T. Maniatis, 1989. Molecular cloning: A laboratory manual, 2nd edn. Cold spring harbor laboratory, cold spring harbor, New York.Sánchez‐Porro, C., S. Martin, E. Mellado and A. Ventosa, 2003. Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. Journal of applied microbiology, 94(2): 295-300.Sarafin, Y., M. B. S. Donio, S. Velmurugan, M. Michaelbabu and T. Citarasu, 2014. Kocuria marina bs-15 a biosurfactant producing halophilic bacteria isolated from solar salt works in India. Saudi journal of biological sciences, 21(6): 511-519.Smibert, R., 1994. Phenotypic characterization. In methods for general and molecular bacteriology. American society for microbiology: 611-651.Solomon, E. and K. J. I. Viswalingam, 2013. Isolation, characterization of halotolerant bacteria and its biotechnological potentials. International journal scientific research paper publication sites, 4: 1-7.Spring, S., W. Ludwig, M. Marquez, A. Ventosa and K.-H. Schleifer, 1996. Halobacillus gen. Nov., with descriptions of Halobacillus litoralis sp. Nov. and Halobacillus trueperi sp. Nov., and transfer of Sporosarcina halophila to Halobacillus halophilus comb. Nov. International journal of systematic evolutionary microbiology, 46(2): 492-496.Tamura, K., D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar, 2011. Mega5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular biology evolution, 28(10): 2731-2739.Yakimov, M. M., K. N. Timmis, V. Wray and H. L. Fredrickson, 1995. Characterization of a new lipopeptide surfactant produced by thermotolerant and halotolerant subsurface Bacillus licheniformis bas50. Applied and environmental microbiology, 61(5): 1706-1713.Yarza, P., P. Yilmaz, E. Pruesse, F. O. Glöckner, W. Ludwig, K.-H. Schleifer, W. B. Whitman, J. Euzéby, R. Amann and R. Rosselló-Móra, 2014. Uniting the classification of cultured and uncultured bacteria and archaea using 16s rRNA gene sequences. Nature reviews microbiology, 12(9): 635-645
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Dissertations / Theses on the topic "Other built environment and design not elsewhere classified"

1

Wong, Kwok Wai Johnny. "Development of selection evaluation and system intelligence analytic models for the intelligent building control systems." Thesis, The Hong Kong Polytechnic University, 2007. https://eprints.qut.edu.au/20343/1/c20343.pdf.

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With the availability of innumerable ‘intelligent’ building products and the dearth of inclusive evaluation tools, design teams are confronted with the quandary of choosing the apposite building control systems to suit the needs of a particular intelligent building project. The paucity of measures that represent the degree of system intelligence and indicate the desirable goal in intelligent building control systems design further inhibits the consumers from comparing numerous products from the viewpoint of intelligence. This thesis is organised respectively to develop models for facilitating the selection evaluation and the system intelligence analysis for the seven predominant building control systems in the intelligent building. To achieve these objectives, systematic research activities are conducted to first develop, test and refine the general conceptual models using consecutive surveys; then, to convert the developed conceptual frameworks to the practical models; and, finally, to evaluate the effectiveness of the practical models by means of expert validations.----- The findings of this study, on one hand, suggest that there are different sets of critical selection criteria (CSC) affecting the selection decision of the intelligent building control systems. Service life, and operating and maintenance costs are perceived as two common CSC. The survey results generally reflect that an ‘intelligent’ building control system does not necessarily need to be technologically advanced. Instead, it should be the one that can ensure efficiency and enhance user comfort and cost effectiveness. On the other hand, the findings of the research on system intelligence suggest that each building control system has a distinctive set of intelligence attributes and indicators. The research findings also indicate that operational benefits of the intelligent building exert a considerable degree of influence on the relative importance of intelligence indicators of the building control systems in the models. This research not only presents a systematic and structured approach to evaluate candidate building control systems against the CSC, but it also suggests a benchmark to measure the degree of intelligence of one control system candidate against another.
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2

(9823415), Darryl O'Brien. "Re-evaluation of the existing change management models to improve the response of the Building Code of Australia to environmental docility." Thesis, 2016. https://figshare.com/articles/thesis/Re-evaluation_of_the_existing_change_management_models_to_improve_the_response_of_the_Building_Code_of_Australia_to_environmental_docility/13387187.

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This thesis presents a conceptual analysis of the Building Code of Australia (BCA) from a change management perspective. The conceptual analysis identifies three principal change management models that have implicitly underpinned the operation of the BCA: logical incrementalism, emergent strategy and co-participative change management. It notes a shift of emphasis in the BCA as it has sought to change from an essentially reactive prescriptive code to one that embraces greater flexibility and proactivity. In order to identify the operational strengths and weaknesses of each of the change management frameworks that underpin the BCA in coping with the more rapid social and technological change that is characteristic of 21st century Australia, each framework was compared in terms of its ability to enable a proactive response to the prevailing BCA hazard identification and risk management requirements. Whilst this analysis showed that the BCA is continuing to provide a generally acceptable built environment it nonetheless identified that the management of change and risk by the BCA is not sufficiently flexible to proactively identify and respond to emerging demographic needs and demands. These weaknesses are particularly evident in situations where building occupants lack the capacity or capability to adapt their behaviour to overcome the restrictions of a BCA compliant built environment in order to satisfy their needs and goals. This condition is known as environmental docility (Lawton, 1974). The limited ability of the BCA highlighted by this analysis pointed to a need to add to the BCA’s underlying conceptual frameworks. Drawing together McLain and Lee’s Adaptive Management Theory (McLain & Lee, 1996), Tolbert & Zucker’s Radical Innovation Management Theory (Tolbert & Zucker, 1966) and Cohen and his colleagues’ work on assessing building performance (Cohen et. al., 2001) a conceptual framework better suited to contemporary needs was proposed. It seeks to provide a conceptual framework for the BCA that enables proactive response to change that draws on past evidence based knowledge and build new knowledge into this evidence base to inform the continuous improvement of the BCA and its application. The thesis proposes practical addition to improve the ability of the BCA to manage more rapid change. This framework is described as an Adaptive and Reflexive Governance Pathway (ARGP). The ARGP seeks to provide a practical theory based approach that supports experimentation and learning as well as active citizen and stakeholder engagement in policy making and implementation whilst maintaining the strengths of building regulation secured by building codes such as the BCA. To test the utility of the AGRP ARGP as an additional methodology to improve the operation of the BCA the thesis reports two proof of concept case studies. The first study considered sleep quality in irregular hour sleepers as an example of a group subject to a type of environmental docility that to date is not recognised by the BCA. The discovery stage of the AGRPARGP identified a large body of research reporting bedroom air temperature, noise and air quality as environmental variables that impact on sleep quality. A mixed method study was undertaken in which shift workers used self-report diaries to record their sleep supported by electronic data loggers to continuously monitor room temperature, noise and air quality. The second case study considered whether the ARGP change management methodology was sufficiently flexible to test the efficacy of historic DtS content that to date had been assumed to meet occupant needs. Using CO2 part per million (ppm) concentrations as a proxy for satisfactory air quality, it tested whether a BCA compliant Class 9b gymnasium building actually provided the ventilation conditions necessary to support active physical exercise by its users. Both of the proof of concept studies illustrated the practical utility of the ARGP to support the data collection and experimentation necessary for proactive change management that enables stakeholder engagement. They provided a first step in the transfer of the ARGP proposed by this thesis into practice. The conceptual analysis of the strengths and weaknesses of the BCA from a change and risk management perspective along with the development of an additional framework and the initial testing of its practical utility provides strong support for the AGRP ARGP as a useful and worthwhile addition to enable the BCA to better address the 21st century demands for building regulation that can proactively support change and active stakeholder engagement.
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(6639611), Christopher D. Munt. ""A Century in the Baths": Allan Bérubé, Spatial Politics and the History of the Bathhouse." Thesis, 2019.

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Building upon and extending a historical narrative composed by Allan Bérubé in 1984, this dissertation interrogates the relationship between physical space and sexual practice by engaging in a historiography of gay bathhouses and by comparing representations of these spaces in the past with contemporary narratives available online. An introduction and conclusion bracket three central chapters, each of which presents findings from a major component of the larger project: The first investigates Bérubé’s sources, methods and underlying political philosophies. The second engages in a case-study of the Bulldog Baths (1979-1982), a popular but short-lived establishment in San Francisco, CA. The third presents findings from a content analysis of contemporary bathhouse websites. Throughout, attention is paid to the active role of physical spaces in sexual encounters taking place in bathhouse settings, as well as to the spatial politics of the urban settings in which these establishments have historically operated.

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(10292846), Zhipeng Deng. "RECOGNITION OF BUILDING OCCUPANT BEHAVIORS FROM INDOOR ENVIRONMENT PARAMETERS BY DATA MINING APPROACH." Thesis, 2021.

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Currently, people in North America spend roughly 90% of their time indoors. Therefore, it is important to create comfortable, healthy, and productive indoor environments for the occupants. Unfortunately, our resulting indoor environments are still very poor, especially in multi-occupant rooms. In addition, energy consumption in residential and commercial buildings by HVAC systems and lighting accounts for about 41% of primary energy use in the US. However, the current methods for simulating building energy consumption are often not accurate, and various types of occupant behavior may explain this inaccuracy.
This study first developed artificial neural network models for predicting thermal comfort and occupant behavior in indoor environments. The models were trained by data on indoor environmental parameters, thermal sensations, and occupant behavior collected in ten offices and ten houses/apartments. The models were able to predict similar acceptable air temperature ranges in offices, from 20.6 °C to 25 °C in winter and from 20.6 °C to 25.6 °C in summer. We also found that the comfortable air temperature in the residences was 1.7 °C lower than that in the offices in winter, and 1.7 °C higher in summer. The reason for this difference may be that the occupants of the houses/apartments were responsible for paying their energy bills. The comfort zone obtained by the ANN model using thermal sensations in the ten offices was narrower than the comfort zone in ASHRAE Standard 55, but that using behaviors was wider.
Then this study used the EnergyPlus program to simulate the energy consumption of HVAC systems in office buildings. Measured energy data were used to validate the simulated results. When using the collected behavior from the offices, the difference between the simulated results and the measured data was less than 13%. When a behavioral ANN model was implemented in the energy simulation, the simulation performed similarly. However, energy simulation using constant thermostat set point without considering occupant behavior was not accurate. Further simulations demonstrated that adjusting the thermostat set point and the clothing could lead to a 25% variation in energy use in interior offices and 15% in exterior offices. Finally, energy consumption could be reduced by 30% with thermostat setback control and 70% with occupancy control.
Because of many contextual factors, most previous studies have built data-driven behavior models with limited scalability and generalization capability. This investigation built a policy-based reinforcement learning (RL) model for the behavior of adjusting the thermostat and clothing level. We used Q-learning to train the model and validated with collected data. After training, the model predicted the behavior with R2 from 0.75 to 0.80 in an office building. This study also transferred the behavior knowledge of the RL model to other office buildings with different HVAC control systems. The transfer learning model predicted with R2 from 0.73 to 0.80. Going from office buildings to residential buildings, the transfer learning model also had an R2 over 0.60. Therefore, the RL model combined with transfer learning was able to predict the building occupant behavior accurately with good scalability, and without the need for data collection.
Unsuitable thermostat settings lead to energy waste and an undesirable indoor environment, especially in multi-occupant rooms. This study aimed to develop an HVAC control strategy in multi-occupant offices using physiological parameters measured by wristbands. We used an ANN model to predict thermal sensation from air temperature, relative humidity, clothing level, wrist skin temperature, skin relative humidity and heart rate. Next, we developed a control strategy to improve the thermal comfort of all the occupants in the room. The control system was smart and could adjust the thermostat set point automatically in real time. We improved the occupants’ thermal comfort level that over half of the occupants reported feeling neutral, and fewer than 5% still felt uncomfortable. After coupling with occupancy-based control by means of lighting sensors or wristband Bluetooth, the heating and cooling loads were reduced by 90% and 30%, respectively. Therefore, the smart HVAC control system can effectively control the indoor environment for thermal comfort and energy saving.
As for proposed studies in the future, at first, we will use more advanced sensors to collect more kinds of occupant behavior-related data. We will expand the research on more occupant behavior related to indoor air quality, noise and illuminance level. We can use these data to recognize behavior instead of questionnaire survey now. We will also develop a personalized zonal control system for the multi-occupant office. We can find the number and location of inlet diffusers by using inverse design.
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5

(11022585), Bhavya Rathna Kota. "Investigation of GenerationZs' perception of Green Homes and Green Home Features." Thesis, 2021.

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In recent years, there has been an increase in environmental awareness in the United States leading to steady growth in environmentally conscious consumerism. These changes have come in response to issues such as the energy crisis, climate change, exponential population growth, and rapid urbanization. This fact is further supported by environmental campaigns and the green movement. Looking to the future of green home marketing, understanding the green consumer behavior of Generation Z (GenZ) is important for environmental and business reasons. The purpose of this research is to better understand the perception of GenZ on Green Homes (GHs). The study uses the lenses of dual inheritance and normative motivation theory to explain the influence of benefits and norms related to environmentalism and sustainability on GenZ consumers’ green behavior. This study seeks to evaluate 1) GenZ’s preferences related to Green Home Features (GHFs), 3) the extent of the influence of certain barriers on the adoption of GHFs, and 3) the types of motivation (intrinsic, instrumental and non-normative) influencing GenZ towards green home consumerism. Data was collected using an online survey questionnaire exclusively at Purdue University during March – April of 2021 (IRB 2020-1414). One hundred sixteen GenZ participants responded to the survey.The findings show that these GenZ consumers prefer a certain type of GHFs over others. Additionally, based on descriptive tests of GHFs, energy-related features were the most prized features, while the least preferred was water-efficient features. Descriptive tests on barriers suggest that GenZ consumers perceive the lack of choice in selecting GHFs in their homes to be a top barrier, followed by a lack of information and the perceived effort to analyze GHFs. Inferential tests for the same indicated that GenZ consumers perceive these barriers differently. Lastly, for GenZ consumers, intrinsic and non-normative motivations significantly affect their willingness to buy GHs. The findings concur with previous studies on green consumer behavior, yet they provide a new benchmark for understanding GenZ consumer behavior on GHs and an updated view of what GHFs they prefer. This research can be used by home marketers and policy makers to study future home trends, attract more potential homeowners to GHs, and help create a sustainable environment for future generations.
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Book chapters on the topic "Other built environment and design not elsewhere classified"

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Boarnet, Marlon, and Randall C. Crane. "An Overview of Travel by Design." In Travel by Design. Oxford University Press, 2001. http://dx.doi.org/10.1093/oso/9780195123951.003.0005.

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Start with the trips people make from home to work, and then back home again. Each commute reflects choices of where to live, where to work, when to work, when to go home, how to get from home to work, and what side trips to make along the way. Each decision depends on the opportunities available, with those in turn explained by the characteristics, resources, and values of workers, their families, their employers, other travelers, and of course the built environment of sidewalks, streets, bus routes, and rail lines connecting home to work. Nonwork trips, the great majority of trips in modern times, entail even more finely detailed mosaics of people, places, and the variety of things one obtains, or hopes to obtain, by going somewhere. Travel is the outcome of a grand confluence of human and other factors, many systematic and many others not. It will never be fully understood. But because travel poses numerous challenges, and opportunities, it would be good to understand more. Planning strategies to reduce traffic congestion and improve air quality continue to get prominent attention. Several increasingly influential efforts emphasize the potentially mitigating role of the built environment. For example, a good deal has been made in recent years of the fact that people drive less and walk more in downtown San Francisco than in suburbs anywhere. Part of this observed behavior is no doubt attributable to the kinds of people living there, people who prefer and indeed seek out the many benefits—travel and otherwise—of a diverse, high-density, mixed-use environment. But many observers have also asked, quite reasonably, if it would not make sense to design suburbs and other neighborhoods to be more like downtown San Francisco, or more like whatever it is about those places that leads people to drive less. Perhaps then people in suburbs and elsewhere would drive less and walk more. And perhaps that would lead to improvements in traffic congestion, air quality, and other transportation problems associated with the automobile.
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Conference papers on the topic "Other built environment and design not elsewhere classified"

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Willey, Lawrence D., and Joel Chalfin. "Electric Power Generation Equipment Specification and Supply in the Rapidly Changing Global Regulatory Scene." In ASME Turbo Expo 2002: Power for Land, Sea, and Air. ASMEDC, 2002. http://dx.doi.org/10.1115/gt2002-30161.

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The proliferation of new codes & standards for power generation equipment procurement, and their increased frequency of revision, contributes to an atmosphere of increasingly rapid change in global trade considerations. This dynamic environment has amplified intensely with each year, to an extent that the life cycle of a given standard is in many instances appreciably less than the delivery cycles of heavy machinery. Other issues are created by the slower pace of harmonization of codes & standards in the European Union (EU), US and elsewhere. These codes & standards cover requirements that include emissions, acoustics, and safety that exert pronounced effects on the design, manufacture, and integration of power plant components. Conformity assessment partnering and the importance of other expert interpretation services are a key component to successfully meeting evolving compliance requirements. Delivering Customer Fulfillment for the Order to Remittance (OTR) phase of a project must be circled back to the Inquiry to Order (ITO) front end of the business cycle for new proposals. Another interesting arena is the relationship of advanced prime mover design balanced with the need for standardization to meet these regulatory challenges in the face of high production volume. The typical power generation project cycle, measured in terms of years, coupled with the present high demand worldwide results in orders for equipment that in many cases can’t foresee regulatory requirements 2 to 3 years into the future. Examples include projects in the EU where the Pressure Equipment Directive (PED) and Atmospheres Explosive (ATEX) Directive have mandatory compliance dates of May 2002 and June 2003 respectively. Electric power generation Original Equipment Manufacturers (OEMs) and their suppliers must plan for and price into contracts compliance with these laws years before the equipment is built and shipped. This is further complicated by the interpretation of specific requirements and the definition of the OEM conformity assessment strategy. To rectify this situation, it is recommended that steps be initiated to accelerate the worldwide harmonization of technical standards. In addition, consideration for the delivery cycles and commissioning of new power plants must be included in the regulatory process and in setting the dates for mandatory compliance with regional law.
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2

Liu, Chengcheng. "Strategies on healthy urban planning and construction for challenges of rapid urbanization in China." In 55th ISOCARP World Planning Congress, Beyond Metropolis, Jakarta-Bogor, Indonesia. ISOCARP, 2019. http://dx.doi.org/10.47472/subf4944.

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In the past 40 years, China has experienced the largest and fastest urbanization development in the world. The infrastructure, urban environment and medical services of cities have been improved significantly. The health impacts are manifested in the decrease of the incidence of infectious diseases and the significant increase of the life span of residents. However, the development of urbanization in China has also created many problems, including the increasing pollution of urban environment such as air, water and soil, the disorderly spread of urban construction land, the fragmentation of natural ecological environment, dense population, traffic congestion and so on. With the process of urbanization and motorization, the lifestyle of urban population has changed, and the disease spectrum and the sequence of death causes have changed. Chronic noncommunicable diseases have replaced acute infectious diseases and become the primary threat to urban public health. According to the data published by the famous medical journal The LANCET on China's health care, the economic losses caused by five major non-communicable diseases (ischemic heart disease, cerebrovascular disease, diabetes mellitus, breast cancer and chronic obstructive pulmonary disease) will reach US$23 trillion between 2012 and 2030, more than twice the total GDP of China in 2015 (US$11.7 trillion). Therefore, China proposes to implement the strategy of "Healthy China" and develop the policy of "integrating health into ten thousand strategies". Integrate health into the whole process of urban and rural planning, construction and governance to form a healthy, equitable and accessible production and living environment. China is building healthy cities through the above four strategies. The main strategies from national system design to local planning are as follows. First of all, the top-level design of the country. There are two main points: one point, the formulation of the Healthy China 2030 Plan determines the first batch of 38 pilot healthy cities and practices the strategy of healthy city planning; the other point, formulate and implement the national health city policy and issue the National Healthy City. The evaluation index system evaluates the development of local work from five aspects: environment, society, service, crowd and culture, finds out the weak links in the work in time, and constantly improves the quality of healthy city construction. Secondly, the reform of territorial spatial planning. In order to adapt to the rapid development of urbanization, China urban plan promote the reform of spatial planning system, change the layout of spatial planning into the fine management of space, and promote the sustainable development of cities. To delimit the boundary line of urban development and the red line of urban ecological protection and limit the disorderly spread of urban development as the requirements of space control. The bottom line of urban environmental quality and resource utilization are studied as capacity control and environmental access requirements. The grid management of urban built environment and natural environment is carried out, and the hierarchical and classified management unit is determined. Thirdly, the practice of special planning for local health and medical distribution facilities. In order to embody the equity of health services, including health equity, equity of health services utilization and equity of health resources distribution. For the elderly population, vulnerable groups and patients with chronic diseases, the layout of community health care facilities and intelligent medical treatment are combined to facilitate the "last kilometer" service of health care. Finally, urban repair and ecological restoration design are carried out. From the perspective of people-oriented, on the basis of studying the comfortable construction of urban physical environment, human behavior and the characteristics of human needs, to tackle "urban diseases" and make up for "urban shortboard". China is building healthy cities through the above four strategies. Committed to the realization of a constantly developing natural and social environment, and can continue to expand social resources, so that people can enjoy life and give full play to their potential to support each other in the city.
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