Relevant bibliographies by topics / Osteogenic therapies

Academic literature on the topic 'Osteogenic therapies'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

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Journal articles on the topic "Osteogenic therapies"

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Helm, Gregory A., Hayan Dayoub, and John A. Jane. "Gene-based therapies for the induction of spinal fusion." Neurosurgical Focus 10, no. 4 (April 2001): 1–5. http://dx.doi.org/10.3171/foc.2001.10.4.6.

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Numerous mesenchymal growth factors with osteogenic properties have now been identified. Although many of these proteins can induce bone formation when delivered on a carrier matrix, these approaches have not been fully developed in the laboratory or clinic. The expression of osteogenic proteins via direct or ex vivo gene therapy techniques is also compelling because high-level, long-term gene expression can now be achieved using novel viral and nonviral vectors. In this brief review the authors will highlight recent advances in genetic therapies for the induction of osteogenesis, as well as their potential use for the promotion of spinal arthrodesis.
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Martin-Iglesias, Sara, Lara Milian, María Sancho-Tello, Rubén Salvador-Clavell, José Javier Martín de Llano, Carmen Carda, and Manuel Mata. "BMP-2 Enhances Osteogenic Differentiation of Human Adipose-Derived and Dental Pulp Stem Cells in 2D and 3D In Vitro Models." Stem Cells International 2022 (March 4, 2022): 1–15. http://dx.doi.org/10.1155/2022/4910399.

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Bone tissue provides support and protection to different organs and tissues. Aging and different diseases can cause a decrease in the rate of bone regeneration or incomplete healing; thus, tissue-engineered substitutes can be an acceptable alternative to traditional therapies. In the present work, we have developed an in vitro osteogenic differentiation model based on mesenchymal stem cells (MSCs), to first analyse the influence of the culture media and the origin of the cells on the efficiency of this process and secondly to extrapolate it to a 3D environment to evaluate its possible application in bone regeneration therapies. Two osteogenic culture media were used (one commercial from Stemcell Technologies and a second supplemented with dexamethasone, ascorbic acid, glycerol-2-phosphate, and BMP-2), with human cells of a mesenchymal phenotype from two different origins: adipose tissue (hADSCs) and dental pulp (hDPSCs). The expression of osteogenic markers in 2D cultures was evaluated in several culture periods by means of the immunofluorescence technique and real-time gene expression analysis, taking as reference MG-63 cells of osteogenic origin. The same strategy was extrapolated to a 3D environment of polylactic acid (PLA), with a 3% alginate hydrogel. The expression of osteogenic markers was detected in both hADSCs and hDPSCs, cultured in either 2D or 3D environments. However, the osteogenic differentiation of MSCs was obtained based on the culture medium and the cell origin used, since higher osteogenic marker levels were found when hADSCs were cultured with medium supplemented with BMP-2. Furthermore, the 3D culture used was suitable for cell survival and osteogenic induction.
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Zaidi, M., N. Zaidi, and L. Sun. "EP receptor agonists and other osteogenic therapies for bone repair." Drugs of the Future 32, no. 9 (2007): 833. http://dx.doi.org/10.1358/dof.2007.032.09.1124489.

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Manochantr, Sirikul, Kulisara Marupanthorn, Chairat Tantrawatpan, Pakpoom Kheolamai, Duangrat Tantikanlayaporn, and Prakasit Sanguanjit. "The Effects of BMP-2, miR-31, miR-106a, and miR-148a on Osteogenic Differentiation of MSCs Derived from Amnion in Comparison with MSCs Derived from the Bone Marrow." Stem Cells International 2017 (2017): 1–14. http://dx.doi.org/10.1155/2017/7257628.

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Mesenchymal stromal cells (MSCs) offering valuable anticipations for the treatment of degenerative diseases. They can be found in many tissues including amnion. MSCs from amnion (AM-MSCs) can differentiate into osteoblast similar to that of bone marrow-derived MSCs (BM-MSCs). However, the ability is not much efficient compared to BM-MSCs. This study aimed to examine the effects of BMP-2 and miRNAs on osteogenic differentiation of AM-MSCs compared to those of BM-MSCs. The osteogenic differentiation capacity after miRNA treatment was assessed by ALP expression, ALP activity, and osteogenic marker gene expression. The results showed that the osteogenic differentiation capacity increased after BMP-2 treatment both in AM-MSCs and BM-MSCs. MiR-31, miR-106a, and miR-148a were downregulated during the osteogenic differentiation. After transfection with anti-miRNAs, ALP activity and osteogenic genes were increased over the time of differentiation. The data lead to the potential for using AM-MSCs as an alternative source for bone regeneration. Moreover, the information of miRNA expression and function during osteogenic differentiation may be useful for the development of new therapeutics or enhanced an in vitro culture technique required for stem cell-based therapies in the bone regeneration.
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Herten, M., M. Sager, L. Benga, J. C. Fischer, M. Jäger, M. Betsch, M. Wild, M. Hakimi, P. Jungbluth, and J. P. Grassmann. "Bone marrow concentrate for autologous transplantation in minipigs." Veterinary and Comparative Orthopaedics and Traumatology 26, no. 01 (2013): 34–41. http://dx.doi.org/10.3415/vcot-11-11-0165.

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SummaryAutologous bone marrow plays an increasing role in the treatment of bone, cartilage and tendon healing disorders. Cell-based therapies display promising results in the support of local regeneration, especially therapies using intra-operative one-step treatments with autologous progenitor cells. In the present study, bone marrow-derived cells were concentrated in a point-of-care device and investigated for their mesenchymal stem cell (MSC) characteristics and their osteogenic potential.Bone marrow was harvested from the iliac crest of 16 minipigs. The mononucleated cells (MNC) were concentrated by gradient density centrifugation, cultivated, characterized by flow cytometry and stimulated into osteoblasts, adipocytes, and chondrocytes. Cell differentiation was investigated by histological and immunohistological staining of relevant lineage markers. The proliferation capacity was determined via colony forming units of fibroblast and of osteogenic alkaline-phosphatase-positive-cells.The MNC could be enriched 3.5-fold in nucleated cell concentrate in comparison to bone marrow. Flow cytometry analysis revealed a positive signal for the MSC markers. Cells could be differentiated into the three lines confirming the MSC character. The cellular osteogenic potential correlated significantly with the percentage of newly formed bone in vivo in a porcine metaphyseal long-bone defect model.This study demonstrates that bone marrow concentrate from minipigs display cells with MSC character and their osteogenic differentiation potential can be used for osseous defect repair in autologous transplantations.
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Favi, Pelagie, Madhu Dhar, Nancy Neilsen, and Roberto Benson. "Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells on Biodegradable Calcium-deficient Hydroxyapatite Tubular Bacterial Cellulose Composites." MRS Proceedings 1621 (2014): 71–79. http://dx.doi.org/10.1557/opl.2014.287.

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ABSTRACTAdvanced biomaterials that mimic the structure and function of native tissues and permit stem cells to adhere and differentiate is of paramount importance in the development of stem cell therapies for bone defects. Successful bone repair approaches may include an osteoconductive scaffold that permits excellent cell adhesion and proliferation, and cells with an osteogenic potential. The objective of this study was to evaluate the cell proliferation, viability and osteocyte differentiation of equine-derived bone marrow mesenchymal stem cells (EqMSCs) when seeded onto biocompatible and biodegradable calcium-deficient hydroxyapatite (CdHA) tubular-shaped bacterial cellulose scaffolds (BC-TS) of various sizes. The biocompatible gel-like BC-TS was synthesized using the bacterium Gluconacetobacter sucrofermentans under static culture in oxygen-permeable silicone tubes. The BC-TS scaffolds were modified using a periodate oxidation to yield biodegradable scaffolds. Additionally, CdHA was deposited in the scaffolds to mimic native bone tissues. The morphological properties of the resulting BC-TS and its composites were characterized using scanning electron microscopy. The ability of the BC-TS and its composites to support and maintain EqMSCs growth, proliferation and osteogenic differentiation in vitro was also assessed. BC-TS and its composites exhibited aligned nanofibril structures. MTS assay demonstrated increasing proliferation and viability with time (days 1, 2 and 3). Cell-scaffold constructs were cultured for 8 days under osteogenic conditions and the resulting osteocytes were positive for alizarin red. In summary, biocompatible and biodegradable CdHA BC-TS composites support the proliferation, viability and osteogenic differentiation of EqMSCs cultured onto its surface in vitro, allowing for future potential use for tissue engineering therapies.
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Littman, Neil, and Arie Abo. "Proceedings: Using Stem Cell Therapies to Reestablish Osteogenic Capability for Bone Regeneration." STEM CELLS Translational Medicine 4, no. 11 (October 19, 2015): 1247–50. http://dx.doi.org/10.5966/sctm.2015-0202.

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Huang, Yueyue, Shuang Wu, Yi Zhou, Yue Zhou, Xiaomei Xu, Dongchuan Zuo, Xiaoping Yuan, and Jin Zeng. "The Effects of Extracts from "Red Complex" Pathogens on Human Dental Follicle Cells." Journal of Biomaterials and Tissue Engineering 10, no. 8 (August 1, 2020): 1128–34. http://dx.doi.org/10.1166/jbt.2020.2370.

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Dental follicle cells could be a natural source of cells for regeneration therapies in periodontitis. The "red complex", which include Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia, is strongly associated with periodontitis. However, the effects of "red complex" on dental follicle cells remains largely unknown. In this study, we evaluated the effects of bacterial extracts obtained from Porphyromonas gingivalis and from "red complex" (Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia in co-culture) on proliferation, migration and osteogenic differentiation of human dental follicle cells. Our data showed that bacterial extracts promoted cell proliferation and migration, decreased the alkaline phosphatase activity, osteogenic genes expression and formation of mineral deposits. Noticeably, compared with the inhibitory effects induced by bacterial extracts from Porphyromonas gingivalis on osteogenic differentiation of human dental follicle cells, the inhibitory effects induced by bacterial extracts from "red complex" were more significant. In conclusion, the extracts from "red complex" pathogens inhibited osteogenic differentiation capacity of human dental follicle cells, and this provides a theoretical basis for the clinical application of stem cell therapy in periodontal tissue engineering.
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Pieles, Oliver, Marcus Höring, Sadiyeh Adel, Torsten E. Reichert, Gerhard Liebisch, and Christian Morsczeck. "Energy Metabolism and Lipidome Are Highly Regulated during Osteogenic Differentiation of Dental Follicle Cells." Stem Cells International 2022 (July 16, 2022): 1–20. http://dx.doi.org/10.1155/2022/3674931.

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Dental follicle cells (DFCs) are stem/progenitor cells of the periodontium and give rise to alveolar osteoblasts. However, understanding of the molecular mechanisms of osteogenic differentiation, which is required for cell-based therapies, is delimited. This study is aimed at analyzing the energy metabolism during the osteogenic differentiation of DFCs. Human DFCs were cultured, and osteogenic differentiation was induced by either dexamethasone or bone morphogenetic protein 2 (BMP2). Previous microarray data were reanalyzed to examine pathways that are regulated after osteogenic induction. Expression and activity of metabolic markers were evaluated by western blot analysis and specific assays, relative amount of mitochondrial DNA was measured by real-time quantitative polymerase chain reaction, the oxidative state of cells was determined by a glutathione assay, and the lipidome of cells was analyzed via mass spectrometry (MS). Moreover, osteogenic markers were analyzed after the inhibition of fatty acid synthesis by 5-(tetradecyloxy)-2-furoic acid or C75. Pathway enrichment analysis of microarray data revealed that carbon metabolism was amongst the top regulated pathways after osteogenic induction in DFCs. Further analysis showed that enzymes involved in glycolysis, citric acid cycle, mitochondrial activity, and lipid metabolism are differentially expressed during differentiation, with most markers upregulated and more markedly after induction with dexamethasone compared to BMP2. Moreover, the cellular state was more oxidized, and mitochondrial DNA was distinctly upregulated during the second half of differentiation. Besides, MS of the lipidome revealed higher lipid concentrations after osteogenic induction, with a preference for species with lower numbers of C-atoms and double bonds, which indicates a de novo synthesis of lipids. Concordantly, inhibition of fatty acid synthesis impeded the osteogenic differentiation of DFCs. This study demonstrates that energy metabolism is highly regulated during osteogenic differentiation of DFCs including changes in the lipidome suggesting enhanced de novo synthesis of lipids, which are required for the differentiation process.
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Gao, Jianliang, Shouyu Xiang, Xiao Wei, Ram Ishwar Yadav, Menghu Han, Weihao Zheng, Lili Zhao, Yichuan Shi, and Yanming Cao. "Icariin Promotes the Osteogenesis of Bone Marrow Mesenchymal Stem Cells through Regulating Sclerostin and Activating the Wnt/β-Catenin Signaling Pathway." BioMed Research International 2021 (January 22, 2021): 1–10. http://dx.doi.org/10.1155/2021/6666836.

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Osteoporosis (OP) is a metabolic disease characterized by decreased bone mass and increased risk of fragility fractures, which significantly reduces the quality of life. Stem cell-based therapies, especially using bone marrow mesenchymal stem cells (BMSCs), are a promising strategy for treating OP. Nevertheless, the survival and differentiation rates of the transplanted BMSCs are low, which limits their therapeutic efficiency. Icariin (ICA) is a traditional Chinese medicine formulation that is prescribed for tonifying the kidneys. It also promotes the proliferation and osteogenic differentiation of BMSCs, although the specific mechanism remains unclear. Based on our previous research, we hypothesized that ICA promotes bone formation via the sclerostin/Wnt/β-catenin signaling pathway. We isolated rat BMSCs and transfected them with sclerostin gene (SOST) overexpressing or knockdown constructs and assessed osteogenic induction in the presence or absence of ICA. Sclerostin significantly inhibited BMSC proliferation and osteogenic differentiation, whereas the presence of ICA not only increased the number of viable BMSCs but also enhanced ALP activity and formation of calcium nodules during osteogenic induction. In addition, the osteogenic genes including Runx2, β-catenin, and c-myc as well as antioxidant factors (Prdx1, Cata, and Nqo1) were downregulated by sclerostin and restored by ICA treatment. Mechanistically, ICA exerted these effects by activating the Wnt/β-catenin pathway. In conclusion, ICA can promote the proliferation and osteogenic differentiation of BMSCs in situ and therefore may enhance the therapeutic efficiency of BMSC transplantation in OP.
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Book chapters on the topic "Osteogenic therapies"

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Nerlich, A., K. Remberger, R. Brenner, M. Lange, and R. K. Müller. "Multifocal Osteogenic Sarcoma in a Patient with Congenital Skeletal Dysplasia Associated with Hyperphosphatasemia." In Fortschritte der Osteologie in Diagnostik und Therapie, 77–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 1988. http://dx.doi.org/10.1007/978-3-642-74004-6_12.

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Datir, Abhijit. "Benign Osteoid Matrix Bone Tumors." In Musculoskeletal Imaging Volume 1, edited by Imran M. Omar, 245–50. Oxford University Press, 2019. http://dx.doi.org/10.1093/med/9780190938161.003.0052.

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Chapter 52 on benign osteoid matrix bone tumors includes osteogenic (meaning osteoid or bony matrix producing) tumors such as osteoid osteoma and osteoblastoma. Osteogenic lesions, or those that form osteoid matrix, are most commonly benign. Enostoses and osteomas are incidentally detected. Osteoid osteomas and osteoblastomas often produce pain and deformity. Important imaging findings that are essential for diagnosis of these entities are discussed with typical examples using radiographs, CT, and MRI. In many cases, imaging can be helpful to direct definitive therapies, such as radiofrequency ablation. A concise discussion on treatment options has also been included.
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Conference papers on the topic "Osteogenic therapies"

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Maynard, Jacqueline A., Ahmad S. Arabiyat, Anna Elefante, Lucas Shearer, Eoin King, and Andrea Kwaczala. "Using Acoustic Waves to Modulate Stem Cell Growth and Differentiation." In ASME 2017 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2017. http://dx.doi.org/10.1115/imece2017-71341.

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During spaceflight, the loss of mechanical loads due to microgravity leads to rapid bone loss, where bone deteriorates at a rate of 1–2% per month, where some astronauts can lose as much as 20% of their skeletal mass in a single expedition [NASA, 2001]. In order to prevent muscle and bone loss, long-term space flight exercise regimes are strictly implemented [Shackleford, 2004]. Current research has demonstrated that mechanical vibrations can help to maintain or improve bone mass [Chan, 2013] and reduce adiposity [Chen, 2015, Sen, 2011] when signals are applied at the appropriate frequency and amplitude. We have developed an acoustic sound chamber that can apply sound waves to stem cells grown in vitro. Characterization of the culture conditions inside the vibration chamber showed considerable variance across the culture plates where an applied acceleration of 0.6g varied at different spots in a 12-well tissue culture plate from as low as 0.47g to 0.78g. We believe the variance is caused by differences in the rigidity of the culture plates that makes the waves transmit inconsistently through the plastic. We hypothesized acoustic waves would induce osteogenic differentiation when applied to stem cells. We utilized pre-osteoblastic stem cells (MC3T3-E1-Subclone 4) to observe the effects of acoustic waves when applied at 0.3g and 0.6g, compared to non-vibrated controls. Cells were vibrated for 30 minutes a day for either 6 days (n = 24/group) or 12 days (n = 12/group). Cellular changes were characterized by assessing well-by-well cell number by a manual cell count and mineral content by Alizarin Red S staining. Differences between groups were determined using One-Way ANOVA with a post hoc test: Student’s t-test. To assess the effects of the variance across the culture plates, correlative analysis was conducted for well-by-well variation using Regression Analysis. Acoustically vibrated wells had 10x more cells after 6 days and showed more mineralization than non-vibrated wells at both 6 and 12 days. Acoustic waves have the ability to increase cell proliferation and can drive stem cell differentiation towards an osteoblastic lineage, this could lead to therapies that prevent bone loss during spaceflight.
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