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1
Zhang, Hanyu, Yuehong Shen, Biru Zhang, Min Qian, Ying Zhang, and Hongyu Yang. "Hsa_circ_0003829 serves as a potential diagnostic predictor for oral squamous cell carcinoma." Journal of International Medical Research 48, no. 9 (September 2020): 030006052093688. http://dx.doi.org/10.1177/0300060520936880.
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Objective Increasing evidence suggests that circular RNAs (circRNAs) play a major role in tumorigenesis and cancer progression. This study aimed to identify aberrant expression of hsa_circ_0003829 in oral squamous cell carcinoma (OSCC) and to explore its clinical significance. Methods We conducted a prospective clinical study to examine the expression pattern of hsa_circ_0003829 in 60 paired OSCC and normal clinical samples and in cell lines using real-time quantitative polymerase chain reaction. We also evaluated the diagnostic value of hsa_circ_0003829 in OSCC based on receiver operating characteristic (ROC) curve analysis, and examined the relationships between hsa_circ_0003829 expression and clinicopathological features in patients with OSCC. We further used bioinformatics software CircInteractome ( https: //Circinteractome.nia.nih.gov/ ) to predict circRNA–microRNA interactions. Results Hsa_circ_0003829 was significantly downregulated in OSCC compared with adjacent normal tissues. The area under the ROC curve was 0.81. Low expression levels of hsa_circ_0003829 in OSCC tissues were negatively correlated with lymph node metastasis status and TNM stage. Conclusions Downregulated expression of has_circ_0003829 suggests that this may be a key circRNA in OSCC, and may serve as a prospective biomarker for the diagnosis of OSCC.
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Wang, Qiang, Lu Zhang, Zhongyi Yan, Longxiang Xie, Yang An, Huimin Li, Yali Han, et al. "OScc: an online survival analysis web server to evaluate the prognostic value of biomarkers in cervical cancer." Future Oncology 15, no. 32 (November 2019): 3693–99. http://dx.doi.org/10.2217/fon-2019-0412.
Full textAbstract:
Aim: To establish a web server that can mutually validate prognostic biomarkers of cervical cancer. Methods: Four datasets including expression profiling and relative clinical follow-up data were collected from Gene Expression Omnibus and The Cancer Genome Atlas. The web server was developed by R software. Results: The web server was named OScc including 690 patients and can be accessed at http://bioinfo.henu.edu.cn/CESC/CESCList.jsp . The Kaplan–Meier survival curves with log-rank p-value and hazard ratio will be generated of interested gene in OScc. Compared with previous predictive tools, OScc had the advantages of registration-free, larger sample size and subgroup analysis. Conclusion: The OScc is highly valuable to perform the preliminary assessment and validation of new or interested prognostic biomarkers for cervical cancer.
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He, Tao, Xiangyu Guo, Xue Li, Chunjuan Liao, Xiaorong Wang, and Kun He. "Plasma-Derived Exosomal microRNA-130a Serves as a Noninvasive Biomarker for Diagnosis and Prognosis of Oral Squamous Cell Carcinoma." Journal of Oncology 2021 (April 16, 2021): 1–9. http://dx.doi.org/10.1155/2021/5547911.
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Exosomal microRNAs (miRNAs) are considered as potential stable biomarkers in many types of human cancer, but investigations of plasma-derived exosomal miRNAs in oral squamous cell carcinoma (OSCC) are still lacking. The aim of this study is to evaluate the diagnostic and prognostic values of exosomal miR-130a in OSCC patients. Exosomes were isolated from plasma samples which were collected from 184 OSCC patients before surgery and 196 healthy individuals. Primary OSCC and paired adjacent noncancerous tissues were also obtained from 47 OSCC patients. The expression levels of miR-130a were analyzed by quantitative real-time PCR (qRT-PCR). Our results showed that the expression levels of exosomal miR-130a were significantly higher in OSCC patients than those of the healthy controls ( p < 0.0001 ). Also, the expression of miR-130a was also significantly upregulated in OSCC tissues compared with paired adjacent noncancerous tissues ( p < 0.0001 ). A significant positive correlation was found between exosomal miR-130a and tissue miR-130a levels. Receiver operating characteristic (ROC) analyses yielded an AUC value of 0.812 in discriminating OSCC patients from healthy controls. Furthermore, high levels of exosomal miR-130a were associated with the late T-stage ( p = 0.024 ), advanced TNM stage ( p = 0.003 ), and poorly differentiated OSCC ( p = 0.013 ). Patients with high exosomal miR-130a expression had significantly worse 3-year overall survival (OS) and recurrence-free survival (RFS). Multivariate analysis indicated that exosomal miR-130a was an independent prognostic factor for OS ( p = 0.001 ) and RFS ( p = 0.003 ). Our results suggest that exosomal miR-130a may serve as a promising diagnostic and prognostic biomarker for OSCC patients.
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Mohanty, Varshasnata, Yashwanth Subbannayya, Shankargouda Patil, Riaz Abdulla, Mandakulutur S. Ganesh, Arnab Pal, Jay Gopal Ray, et al. "Molecular alterations in oral cancer between tobacco chewers and smokers using serum proteomics." Cancer Biomarkers 31, no. 4 (July 16, 2021): 361–73. http://dx.doi.org/10.3233/cbm-203077.
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BACKGROUND: Tobacco exposure (through smoking or chewing) is one of the predominant risk factors associated with the development of oral squamous cell carcinoma (OSCC). Despite the growing number of patients diagnosed with OSCC, there are few circulating biomarkers for identifying individuals at a higher risk of developing the disease. Successful identification of candidate molecular markers for risk assessment could aid in the early detection of oral lesions and potentially be used for community screening of high-risk populations. OBJECTIVE: Identification of differentially expressed proteins in the serum of oral cancer patients which can serve as biomarkers for the diagnosis of the onset of oral cancer among tobacco users. METHODS: We employed a tandem mass tag (TMT)-based quantitative proteomics approach to study alterations in the serum proteomes of OSCC patients based on their tobacco exposure habits (chewing and smoking) compared to healthy individuals with no history of using any form of tobacco or any symptoms of the disease. RESULTS: Mass spectrometry-based analysis resulted in the identification of distinct signatures in the serum of OSCC patients who either chewed or smoked tobacco. Pathway analysis revealed opposing effects of dysregulated proteins enriched in the complement-coagulation signaling cascades with a high expression of the Serpin family of proteins observed in OSCC patients who chewed tobacco compared to healthy individuals whereas these proteins showed decreased levels in OSCC patients who smoked. ELISA-based validation further confirmed our findings revealing higher expression of SERPINA6 and SERPINF1 across serum of OSCC patients who chewed tobacco compared to healthy individuals. CONCLUSIONS : This study serves as a benchmark for the identification of serum-based protein markers that may aid in the identification of high-risk patients who either chew tobacco or smoke tobacco.
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Narayanan, Maithrea Suresh, Nur Karyatee Kassim, Tang Liszen, Baharuddin Abdullah, Julia Omar, Suhaily Mohd Hairon, and Norhafiza Mat Lazim. "The Utility of Beta 2 Microglobulin (B2M) as an Initial Diagnostic Tool for Oral Squamous Cell Carcinoma (OSCC): Evidence from a Malaysian Scenario." Bangladesh Journal of Medical Science 18, no. 4 (August 30, 2019): 729–35. http://dx.doi.org/10.3329/bjms.v18i4.42876.
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Background: The delay in diagnosis of oral squamous cell carcinoma (OSCC) is a factor in rendering the poor prognosis, and recent research has explored the use of serum tumour markers such Beta 2 Microglobulin (B2M), to aid early diagnosis. However, despite a high incidence of OSCC in Southeast Asia, no studies on the clinical use of B2M in the region were found.
Objectives: To determine if serum B2M level can serve as an initial diagnostic tool to indicate if a biopsy is warranted, and if so, to propose a local B2M serum reference value to identify OSSC patients.
Methodology: Twenty-one patients were seen at Hospital Universiti Sains Malaysia (HUSM) for a one-year period, between June 2016 and June 2017, and equal number of healthy controls participated in the study. Apart from patient history, venous blood of approximately 5ml volume was collected from each subject at the pre-treatment stage and analysed by an Abbot ARCHITECT c8000 analyser using the immunoturbidimetry method. The results were analysed using ROC analysis and the Mann Whitney test.
Results: Serum B2M levels showed a statistically significant increase (p<0.001) in patients compared to controls. The test was shown to have 90.5% sensitivity and 90.5% specificity. It was found to be a sensitive and specific serum tumour marker at a cut off value of 1.57mg/l to differentiate cases from controls.
Conclusion: B2M is a sensitive and specific tumour marker to differentiate OSCC cases from controls. It is cost effective and minimally invasive, making it a potentially useful adjunct diagnostic tool in a high-risk patient pool.
Bangladesh Journal of Medical Science Vol.18(4) 2019 p.729-735
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Yan, Xiaodong, Bin Cong, Qinchao Chen, Lingyun Liu, Xidi Luan, Jianxin Du, and Meng Cao. "Silencing lncRNA HOXA10-AS decreases cell proliferation of oral cancer and HOXA10-antisense RNA can serve as a novel prognostic predictor." Journal of International Medical Research 48, no. 8 (August 2020): 030006052093425. http://dx.doi.org/10.1177/0300060520934254.
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Objective Long noncoding (lnc)RNAs regulate multiple biological processes including cancer. Oral squamous cell carcinoma (OSCC) is a common malignancy with poor prognosis. We aimed to identify the function of lncRNA HOXA10 antisense RNA (HOXA10-AS) and its clinical significance. Methods We used differential expression analysis to identify aberrantly expressed lncRNAs associated with OSCC. We identified key genes related to HOXA10-AS and their biological functions using bioinformatics tools and functional enrichment analyses. We predicted the function of HOXA10-AS using gene set enrichment and variation analyses and analyzed proliferation markers at the mRNA and protein levels. Finally, we silenced HOXA10-AS using antisense oligonucleotide and assessed proliferation ability using a cell counting kit (CCK8) and clone formation assays. Results In total, 506 aberrantly expressed lncRNAs were identified. HOXA10-AS was identified as a risk factor for OSCC and its expression was positively associated with tumor grade. We identified hub genes involved in regulating proliferation and predicted that HOXA10-AS is associated with an active cell cycle and increased proliferation. Silencing HOXA10-AS decreased proliferation in OSCC cell lines. Conclusions HOXA10-AS is involved in cell proliferation and silencing it decreases proliferation. Thus, HOXA10-AS could serve as prognostic biomarker and therapeutic target for OSCC.
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Jiang, L., H. S. Yang, Z. Wang, Y. Zhou, M. Zhou, X. Zeng, and Q. M. Chen. "ORAOV1-A Correlates with Poor Differentiation in Oral Cancer." Journal of Dental Research 88, no. 5 (May 2009): 433–38. http://dx.doi.org/10.1177/0022034509336994.
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Oral cancer overexpressed 1 ( ORAOV1) is a crucial oncogene in oral squamous cell carcinoma (OSCC). In this study, we have identified a novel splice variant of ORAOV1, designated as ORAOV1-A. To study the potential role of ORAOV1-A in OSCC, we tested its expression in 7 OSCC cell lines, as well as in 19 normal oral tissue samples and 47 OSCC tissue samples. The expression of ORAOV1-A was detectable in 6 out of 7 OSCC cell lines tested. In OSCC tissue samples, the expression frequency of ORAOV1-A (51.1%) was much higher than that in normal samples (10.5%). Notably, an inverse correlation was found between the expression frequency of ORAOV1-A and the degree of differentiation in OSCC ( P = 0.0017). In conclusion, our results suggested that ORAOV1-A may play a functional role in the tumorigenesis of OSCC, and ORAOV1-A expression may serve as an adjunctive prognostic indicator for persons with OSCC.
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Zomlot, Husam Said. "Building a state under occupation: the Palestinians and the living legacy of Oslo*." Contemporary Arab Affairs 3, no. 2 (April 1, 2010): 180–92. http://dx.doi.org/10.1080/17550911003743917.
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The Palestine National Movement has been engaged in ‘state-building’ in the absence of a state since 1969: in exile (1969–1993) and under Israel's occupation since 1994. Whereas the pre-Oslo ‘state in exile’ was a voluntary act that served several crucial functions including reinforcing Palestinian identity and entity, the post-Oslo state-building has been an obligatory exercise dictated by the terms of the Oslo interim agreements. This paper examines the framework of the post-Oslo state-building and highlights the inherent tensions between the function of the Palestinian Authority as a depository of the anticipated state and the tasks of ending occupation and nation-building. It scrutinizes the international financial role (the post-Oslo international aid program) and argues for a reassessment of international involvement.
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Yang, Lan, Youyuan Wang, Lvhua Guo, Liping Wang, Weiliang Chen, and Bin Shi. "The Expression and Correlation of iNOS and p53 in Oral Squamous Cell Carcinoma." BioMed Research International 2015 (2015): 1–8. http://dx.doi.org/10.1155/2015/637853.
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Oral squamous cell carcinoma (OSCC) is the most prevalent form of oral cancer. Inducible nitric oxide synthase (iNOS) and p53 are associated with a variety of human cancers, but their expression and interaction in OSCC have not been fully explored. In this study, we investigated the expression of iNOS and p53 in OSCC and their correlation with tumor development and prognosis. In addition, we explored the interaction of iNOS and p53 in OSCC. The expression of iNOS and p53 in OSCC was investigated using immunohistochemical method and their interaction was studied using RNAi technique. Our results showed that the expression of both iNOS and p53 was significantly correlated with tumor stages and pathological grade of OSCC (P<0.05). In contrast, there was no correlation between iNOS and p53 expression and lymph node metastasis (P<0.05). The OSCC survival rate was negatively associated with iNOS expression, but not with p53. A significant increase in the expression of the p53 was observed when iNOS expression was knocked down. The immunoexpression of iNOS is correlated with tumorigenesis and prognosis of OSCC and may serve as a prognostic marker.
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Mehterov, Nikolay, Boyan Vladimirov, Andrea Sacconi, Claudio Pulito, Marcin Rucinski, Giovanni Blandino, and Victoria Sarafian. "Salivary miR-30c-5p as Potential Biomarker for Detection of Oral Squamous Cell Carcinoma." Biomedicines 9, no. 9 (August 24, 2021): 1079. http://dx.doi.org/10.3390/biomedicines9091079.
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The levels of different classes of extracellular RNAs (exRNAs) remain stable in bodily fluids. The detection of either enriched or depleted specific subsets of salivary microRNAs (miRNAs) has the potential to serve as a non-invasive approach for biomarker development. Thus, salivary miRNAs have emerged as a promising molecular tool for early diagnosis and screening of oral squamous cell carcinoma (OSCC). Total RNA was extracted from saliva supernatant of 33 OSCC patients and 12 controls (discovery set), and the differential expression of 8 cancer-related miRNAs was detected by TaqMan assay. Among the screened miRNAs, miR-30c-5p (p < 0.04) was significantly decreased in OSCC saliva. The same transcriptional behavior of miR30c-5p was observed in an additional validation set. miR-30c-5p showed a significant statistical difference between cases and controls with areas under the curve (AUC) of 0.82 (95% CI: 0.71–0.89). The sensitivity and the specificity of miR-30c-5p were 86% and 74%, respectively. The target identification analysis revealed enrichment of miR-30c-5p targets in p53 and Wnt signaling pathways in OSCC. Additionally, the miR-30c-5p targets had clinical significance related to overall survival. In conclusion, these findings show that downregulated miR-30c-5p has the potential to serve as a novel, non-invasive biomarker for early OSCC detection.
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Przeperski, Michał. "Dyplomacja na peryferiach. „Specjalne kontakty” polsko-norweskie w latach 60. XX wieku." Studia Scandinavica, no. 2 (22) (December 28, 2018): 168–80. http://dx.doi.org/10.26881/ss.2018.22.10.
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“Special contacts” developed by Norway and Poland during the years 1963–1968 may serve as a unique example of peripheral diplomacy. The sense of Polish initiatives such as the Gomułka Plan consisted not only in relaxing the political tension in Europe, but also on securing the western Polish border, which was not internationally recognized by the West. Hence, initially, political contacts between Oslo and Warsaw served as a way of exercising soft pressure on NATO countries to change their stance. However, they had other interesting aspects, too, such as promotion of Polish culture in Norway. It was 1968 that marked a final date of the “special contact.” It was the anti-Semitic campaign that took place in Poland that year, rather than invasion of Czechoslovakia, that was a final reason for this ending.
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Xiao, Yao, Hao Li, Lei-Lei Yang, Liang Mao, Cong-Cong Wu, Wen-Feng Zhang, and Zhi-Jun Sun. "The Expression Patterns and Associated Clinical Parameters of Human Endogenous Retrovirus-H Long Terminal Repeat-Associating Protein 2 and Transmembrane and Immunoglobulin Domain Containing 2 in Oral Squamous Cell Carcinoma." Disease Markers 2019 (April 7, 2019): 1–9. http://dx.doi.org/10.1155/2019/5421985.
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Human endogenous retrovirus-H long terminal repeat-associating protein 2 (HHLA2) and transmembrane and immunoglobulin domain containing 2 (TMIGD2) are new immune checkpoint molecules of the B7:CD28 family; however, little research has been performed on these immune checkpoint molecules. In this study, we used oral squamous cells carcinoma (OSCC) tissue microarrays and immunohistochemistry methods to investigate the expression patterns of HHLA2 and TMIGD2 in OSCC. After comparing the HHLA2 and TMIGD2 expression levels in OSCC, dysplasia, and mucosa, we found increased HHLA2 expression in OSCC and dysplasia, while the TMIGD2 expression was decreased in OSCC and dysplasia. Using the Kaplan-Meier method and log-rank test, we found that higher HHLA2 or TMIGD2 expression levels in OSCC indicate poor prognosis. Furthermore, two-tailed Pearson’s statistical analysis revealed that the HHLA2 expression levels in OSCC, dysplasia, and mucosa were positively correlated with the T cell immunoglobulin and mucin-domain containing-3 (TIM3), lymphocyte-activation gene 3 (LAG3), B7 homolog 3 protein (B7-H3), B7 homolog 4 protein (B7H4), and V-domain Ig suppressor of T cell activation (VISTA) levels, while the TMIGD2 expression levels in OSCC, dysplasia, and mucosa were inversely correlated with the TIM3, LAG3, and B7H3 levels. Our current study demonstrates that HHLA2 may serve as an immune target for OSCC therapy and that the TMIGD2 expression level in OSCC could forecast patient prognosis.
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Liang, Xiaowei, Zhizhao Chen, and Geng Wu. "FOXD2-AS1 Predicts Dismal Prognosis for Oral Squamous Cell Carcinoma and Regulates Cell Proliferation." Cell Transplantation 29 (January 1, 2020): 096368972096441. http://dx.doi.org/10.1177/0963689720964411.
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The roles of long noncoding RNA FOXD2 adjacent opposite strand RNA 1 (FOXD2-AS1) in oral squamous cell carcinoma (OSCC) remain largely unknown. Here, the Atlas of Noncoding RNAs in Cancer online database was utilized to analyze the expression and clinical significance of FOXD2-AS1 in OSCC. Then, the cell proliferation of FOXD2-AS1-silenced OSCC cells (CAL-27) was assessed by MTT and clone formation experiments. FOXD2-AS1-coexpressed genes were enriched and analyzed via circlncRNAnet and Metascape tools. Finally, key molecules of the signal pathways of the aforementioned coexpressed genes were verified by western blotting. We found that FOXD2-AS1 was significantly highly expressed in OSCC tissues, and correlated with poor pathological grade and prognosis in patients with OSCC. Cell viability and clone formation ability were significantly inhibited after the knockdown of FOXD2-AS1. A total of 32 coexpressed genes of FOXD2-AS1 were identified, and those genes were enriched in the cell cycle. In conclusion, FOXD2-AS1 may be served as a potential prognostic indicator and therapeutic target for OSCC.
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Fande, Prajakta Zade, Minal S. Chaudhary, Alka H. Hande, Madhuri N. Gawande, Amol R. Gadbail, Preethi N. Sharma, and Swati K. Patil. "A compendium of population wide DNA methylation profile for oral cancer in India." International Journal of Molecular & Immuno Oncology 6 (May 29, 2021): 82–88. http://dx.doi.org/10.25259/ijmio_2_2021.
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With the emergence of epigenetics, constant attempts are been made to decipher the molecular mechanisms in carcinogenesis. Epigenetic modifications, especially the DNA methylation, have been perceived in oral squamous cell carcinoma (OSCC). The target genes differentially methylated in OSCC still largely remains unknown. There are differences in the molecular alterations in OSCC, regarding geographic location. Therefore, the aim of this review is to present status-quo of existing studies on Indian population to better understand the aberrant patterns of DNA methylation in OSCC that could serve as potential prognostic and diagnostic biomarkers to improve therapy and extend overall survival. The literature was searched using MEDLINE/PubMed, Wiley, Google Scholar, and Science Direct to identify and include most of the relevant articles published from the year 2000 till date in English language. The review would prove to be a valuable resource for population specific investigations and detecting novel biomarkers for OSCC.
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Lin, Li-Han, Kuo-Wei Chang, Shou-Yen Kao, Hui-Wen Cheng, and Chung-Ji Liu. "Increased Plasma Circulating Cell-Free DNA Could Be a Potential Marker for Oral Cancer." International Journal of Molecular Sciences 19, no. 11 (October 24, 2018): 3303. http://dx.doi.org/10.3390/ijms19113303.
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Background: Oral squamous cell carcinoma (OSCC) is a disease that affects patients worldwide. DNA of dead cells is released into the blood stream and may be isolated from plasma or serum samples. This DNA is termed cell-free DNA (cfDNA). cfDNA is increased in several types of malignancies. We investigated if there was a correlation between cfDNA levels and the progression of OSCC. Methods: Using quantitative spectrometry, we measured plasma cfDNA in 121 patients with OSCC and 50 matched controls. Mann Whitney and Wilcoxon tests were used to compare differences among various clinical variants. Receiver operating characteristic (ROC) analysis was used to obtain levels suitable for the separation of the clinical subsets. Kaplan-Meier analysis was used to assess correlation with survival. Results: Plasma cfDNA was significantly elevated in patients with OSCC relative to controls. Plasma cfDNA levels correlated with larger tumor size, cervical lymph node metastasis and late stage. Higher plasma cfDNA levels were associated with a poor prognosis of OSCC, which is a new finding. Conclusion: Plasma cfDNA could serve as a novel and easily accessible biomarker in OSCC, providing diagnostic and prognostic value.
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Lin, Yueh-Min, Chiao-Wen Lin, Jeng-Wei Lu, Kun-Tu Yeh, Shu-Hui Lin, and Shun-Fa Yang. "Decreased Cytoplasmic Expression of ADAMTS14 Is Correlated with Reduced Survival Rates in Oral Squamous Cell Carcinoma Patients." Diagnostics 10, no. 2 (February 23, 2020): 122. http://dx.doi.org/10.3390/diagnostics10020122.
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A disintegrin and metalloproteinase with thrombospondin motif 14 (ADAMTS14) is a member of the zinc-dependent protease family that is implicated in the occurrence and progression of tumors. Oral cancer (OC) is a common cancer worldwide, but it is particularly prevalent in Taiwan. However, whether the expression of ADAMTS14 is correlated with the carcinogenesis and progression of oral squamous cell carcinoma (OSCC) has not yet been investigated. In this study, we used immunohistochemistry (IHC) to examine 250 OSCC specimens in order to identify correlations between the cytoplasmic expression of ADAMTS14 and (1) clinicopathological features of OSCC as well as (2) clinical outcomes of OSCC. Our results indicate that cytoplasmic expression of ADAMTS14 was lower in OSCC tissues than in normal tissues. In analyzing correlations between ADAMTS14 expression and clinicopathological features, we found that negative cytoplasmic expression of ADAMTS14 was significantly associated with higher frequencies of lymph node metastasis and more advanced AJCC stages (III/IV). Kaplan–Meier survival analysis revealed that negative cytoplasmic expression of ADAMTS14 was also associated with significantly worse OSCC survival. Univariate and multivariate analyses confirmed that cytoplasmic expression of ADAMTS14 was associated with lymph node metastasis, tumor stage, and tumor grade and also indicated that cytoplasmic ADAMTS14 expression may be an independent prognostic factor for OSCC. This is the first study to report that the cytoplasmic expression level of ADAMTS14 is associated with OSCC prognosis and tumor progression. Our data indicate that ADAMTS14 can serve as a prognostic marker and a potential therapeutic target for OSCC.
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Vincent-Chong, Vui King, and Mukund Seshadri. "Development and Radiation Response Assessment in A Novel Syngeneic Mouse Model of Tongue Cancer: 2D Culture, 3D Organoids and Orthotopic Allografts." Cancers 12, no. 3 (March 2, 2020): 579. http://dx.doi.org/10.3390/cancers12030579.
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Oral squamous cell carcinoma (OSCC) are aggressive cancers that contribute to significant morbidity and mortality in humans. Although numerous human xenograft models of OSCC have been developed, only a few syngeneic models of OSCC exist. Here, we report on a novel murine model of OSCC, RP-MOC1, derived from a tongue tumor in a C57Bl/6 mouse exposed to the carcinogen 4-nitroquinoline-1-oxide. Phenotypic characterization and credentialing (STR profiling, exome sequencing) of RP-MOC1 cells was performed in vitro. Radiosensitivity was evaluated in 2D culture, 3D organoids, and in vivo using orthotopic allografts. RP-MOC1 cells exhibited a stable epithelial phenotype with proliferative, migratory and invasive properties. Exome sequencing identified several mutations commonly found in OSCC patients. The LD50 for RP-MOC1 cells in 2D culture and 3D organoids was found to be 2.4 Gy and 12.6 Gy, respectively. Orthotopic RP-MOC1 tumors were pan-cytokeratin+ and Ki-67+. Magnetic resonance imaging of orthotopic RP-MOC1 tumors established in immunocompetent mice revealed marked growth inhibition following 10 Gy and 15 Gy fractionated radiation regimens. This radiation response was completely abolished in tumors established in immunodeficient mice. This novel syngeneic model of OSCC can serve as a valuable platform for the evaluation of combination strategies to enhance radiation response against this deadly disease.
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Li, Bowen, Feng Wang, Xiang Li, Shuai Sun, Yuehong Shen, and Hongyu Yang. "Hsa_circ_0008309 May Be a Potential Biomarker for Oral Squamous Cell Carcinoma." Disease Markers 2018 (September 23, 2018): 1–8. http://dx.doi.org/10.1155/2018/7496890.
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Objective. Oral squamous cell carcinoma (OSCC) is the most common cancer of the head and neck region. The circular RNA (circRNA) is known to serve an important role in the carcinogenesis of different types of cancer. However, the circRNA role of OSCC remains unclear. Methods. 8 pairs of OSCC tissues and adjacent normal tissues were obtained to detect circRNAs expression by high-throughput sequencing, and 45 pairs of OSCC tissues were selected to verify the differentially significant circRNAs by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). To further investigate the role of hsa_circ_0008309, the circRNA-microRNA (miR)-mRNA network was predicted using bioinformatics databases. The expression levels of hsa_circ_0008309, miR-1290, miR-136-5P, and miR-382-5P in SCC-15 and CAL27 cell lines were detected by RT-qPCR. Western blotting was performed to detect the protein level of Ataxin 1 (ATXN1). Results. The high-throughput sequencing results demonstrated that circRNAs were abundantly expressed in OSCC, and 16 circRNAs were significantly differentially expressed. Hsa_circ_0008309 was significantly downregulated in 45 pairs of OSCC tissue samples and was statistically correlated with pathological differentiation. The bioinformatics databases suggested that hsa_circ_0008309 could combine with miR-1290, miR-136-5P, and miR-382-5P, respectively, to regulate the expression of ATXN1. It was subsequently identified that hsa_circ_0008309 may inhibit miR-136-5P and miR-382-5P expression and increase ATXN1 expression in the OSCC cell lines. Conclusion. In summary, the results of the present study revealed that OSCC tissues have abundant circRNAs and, to the best of our knowledge, we firstly explore the regulatory role of the hsa_circ_0008309-miR-136-5P/hsa-miR-382-5P-ATXN1 network in OSCC. The results indicated that hsa_circ_0008309 may be a potential biomarker for OSCC.
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Wong, Yin-Ling, Anand Ramanathan, Kar Mun Yuen, Wan Mahadzir Wan Mustafa, Mannil Thomas Abraham, Keng Kiong Tay, Zainal Ariff Abdul Rahman, and Yeng Chen. "Comparative sera proteomics analysis of differentially expressed proteins in oral squamous cell carcinoma." PeerJ 9 (June 10, 2021): e11548. http://dx.doi.org/10.7717/peerj.11548.
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Background Oral squamous cell carcinoma (OSCC) has increased in incidence from 1990 to 2017, especially in South and Southeast Asia. It is often diagnosed at an advanced stage with a poor prognosis. Therefore, early detection of OSCC is essential to improve the prognosis of OSCC. This study aims to identify the differentially expressed serum proteins as potential biomarkers for oral squamous cell carcinoma (OSCC). Methods Comparative proteomics profiling of serum samples from OSCC patients, oral potentially malignant disorder (OPMD) patients, and healthy individuals were performed using two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) (n = 60) and bioinformatics analysis. The enzyme-linked immunosorbent assay (ELISA) (n = 120) and immunohistochemistry (IHC) (n = 70) were used to confirm our findings. Results The 2-DE analysis revealed that 20 differentially expressed proteins were detected in OPMD and OSCC (p < 0.05). Bioinformatics analysis indicated that the activation of classical complement, liver X receptor/retinoid X receptor (LXR/RXR) activation, and acute phase response signaling pathway are associated with the development and progression of OSCC. Most of the detected proteins are acute-phase proteins and were related to inflammation and immune responses, including apolipoprotein A-I (APOA1), complement C3 (C3), clusterin (CLU), and haptoglobin (HP). The expression levels of CLU and HP in ELISA are consistent with the findings from the 2-DE analysis, except for the mean serum level of HP in OPMD, whereby it was slightly higher than that in control. IHC results demonstrated that CLU and HP are significantly decreased in OSCC tissues. Conclusion Decreased expression of CLU and HP could serve as complementary biomarkers of OSCC. These proteins may assist in predicting the outcomes of OSCC patients. However, a larger cohort is needed for further investigation.
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Dong, Yunze, Yuchen Gao, Tiancheng Xie, Huan Liu, Xiangcheng Zhan, and Yunfei Xu. "miR-101-3p Serves as a Tumor Suppressor for Renal Cell Carcinoma and Inhibits Its Invasion and Metastasis by Targeting EZH2." BioMed Research International 2021 (July 7, 2021): 1–12. http://dx.doi.org/10.1155/2021/9950749.
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Background. The role of miRNAs in renal cell carcinoma (RCC) is not certain. We wanted to study the biological functions and potential mechanisms of miR-101-3p in RCC. Methods. miR-101-3p was inhibited in A498 and OSRC-2 (two RCC cell lines). We studied its effect on cell invasion and proliferation. Target EZH2 of miR-101-3p was designated by different methods, including luciferase functional analysis and Western blotting. The expression level of the target gene in treated cells was quantitatively analyzed by quantitative real-time polymerase chain reaction. In addition, induction of miR-101-3p to prevent tumor formation of A498 cells in mice was further studied. Results. The overexpression of miR-101-3p significantly inhibited the proliferation, migration, and invasion in two RCC cells. Western blotting and luciferase functional analysis indicated that miR-101-3p regulated the expression of EZH2 in two cell lines. Mice inoculated with A498 and OSRC-2 cells transfected with miR-101-3p mimics showed significantly smaller xenografts and weaker EZH2 expression levels than the control group. Conclusions. miR-101-3p inhibited RCC cell proliferation, migration, and invasion by targeting EZH2.
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Tsai, Cheng-Kun, Chien-Yu Lin, Chung-Jan Kang, Chun-Ta Liao, Wan-Ling Wang, Meng-Han Chiang, Tzu-Chen Yen, and Gigin Lin. "Nuclear Magnetic Resonance Metabolomics Biomarkers for Identifying High Risk Patients with Extranodal Extension in Oral Squamous Cell Carcinoma." Journal of Clinical Medicine 9, no. 4 (March 30, 2020): 951. http://dx.doi.org/10.3390/jcm9040951.
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Extranodal extension (ENE) is an independent adverse prognostic factor in oral squamous cell carcinoma (OSCC), and is difficult to identify preoperatively. We aimed to discover biomarkers for high risk patients with ENE. Tandem tissue, plasma, and urine samples of 110 patients with OSCC were investigated through 600-MHz nuclear magnetic resonance (NMR) metabolomics analysis. We found that the levels of creatine, creatine phosphate, glycine, and tyramine in plasma significantly decreased in stage IV ENE positive OSCC compared with stage IV ENE negative OSCC. To understand the underlying mechanism behind the alteration of plasma metabolites, our tissue analysis revealed that the carnitine level significantly increased in tumors but significantly decreased in the adjacent normal tissue in advanced stage OSCC, in addition to decreased levels of alanine and pyruvate in tumor tissues. The global metabolomics analysis on tumor tissues also showed that stage IV tumors with an ENE positive status demonstrated higher levels of aspartate, butyrate, carnitine, glutamate, glutathione, glycine, glycolate, guanosine, and sucrose but lower levels of alanine, choline, glucose, isoleucine, lactate, leucine, myo-inositol, O-acetylcholine, oxypurinol, phenylalanine, pyruvate, succinate, tyrosine, valine, and xanthine than tumors with an ENE negative status. We concluded that metabolomics alterations in tumor tissues correspond to an increase in the tumor stage and are detectable in plasma samples. Metabolomic alterations of OSCC can serve as potential diagnostic markers and predictors of ENE in patients with stage IV OSCC.
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Tilly, Kit, Aaron Bestor, and Patricia A. Rosa. "Functional Equivalence of OspA and OspB, but Not OspC, in Tick Colonization by Borrelia burgdorferi." Infection and Immunity 84, no. 5 (March 7, 2016): 1565–73. http://dx.doi.org/10.1128/iai.00063-16.
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Borrelia burgdorferi, a Lyme disease agent, makes different major outer surface lipoproteins at different stages of its mouse–tick infectious cycle. Outer surface protein A (OspA) coats the spirochetes from the time they enter ticks until they are transmitted to a mammal. OspA is required for normal tick colonization and has been shown to bind a tick midgut protein, indicating that OspA may serve as a tick midgut adhesin. Tick colonization by spirochetes lacking OspA is increased when the infecting blood meal is derived from mice that do not produce antibody, indicating that OspA may protect the spirochetes from host antibody, which will not recognize tick-specific proteins such as OspA. To further study the importance of OspA during tick colonization, we constructed a form ofB. burgdorferiin which theospAopen reading frame, on lp54, was replaced with theospCgene or theospBgene, encoding a mammal-specific or tick-specific lipoprotein, respectively. These fusions yielded a strain that produces OspC within a tick (from the fusion gene) and during early mammalian infection (from the normalospClocus) and a strain that produces OspB in place of OspA within ticks. Here we show that the related, tick-specific protein OspB can fully substitute for OspA, whereas the unrelated, mammal-specific protein OspC cannot. These data were derived from three different methods of infecting ticks, and they confirm and extend previous studies indicating that OspA both protects spirochetes within ticks from mammalian antibody and serves an additional role during tick colonization.
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Dana, Karam, and Hannah Walker. "Invisible disasters: the effects of Israeli occupation on Palestinian gender roles." Contemporary Arab Affairs 8, no. 4 (October 1, 2015): 488–504. http://dx.doi.org/10.1080/17550912.2015.1090100.
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Women's participation in the First Intifada allowed for increased gender equality in Palestine. However, the weakness of the Palestinian Authority, established by the Oslo Accords, created space for non-state actors (dominated by the Islamist political organization Hamas) to emerge and gain popularity. Likewise, during the post-Oslo period conservative positions on gender resurged. This paper re-examines the structural factors that facilitated increased gender inequality and argues that the nature of the occupation itself serves as the greatest force for gender inequality in Palestine. To develop and test our theory, we draw on original, large-n survey data and in-depth interviews.
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Gao, Feng, Panpan Yin, Yanlin Wu, Jinlin Wen, Ying Su, and Xinyan Zhang. "Knockdown of RhoC Inhibits Oral Squamous Cell Carcinoma Cell Invasion and Metastasis via Regulation of HMGA2." Journal of Oncology 2021 (January 12, 2021): 1–12. http://dx.doi.org/10.1155/2021/6644077.
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Ras homolog family member C (RhoC) is an important component of intracellular signal transduction and its overexpression has been reported to be involved in regulating tumor proliferation, invasion, and metastasis in various malignant tumors. However, its role and underlying mechanism in oral squamous cell carcinoma (OSCC) still remain obscure. In our study, RhoC expression, its relation with clinical stages, and survival rate in OSCC were analyzed using datasets from The Cancer Genome Atlas (TCGA). Next, a RhoC knockdown cell model was established in vitro, and the effects of RhoC knockdown in OSCC cells were detected by the MTT assay, colony formation assay, transwell invasion assay, scratch assay, and F-actin phalloidin staining. An in vivo tongue-xenografted nude mouse model was established to measure the effects of knockdown of RhoC on tumor cell growth and lymph node metastasis. A mechanism study was conducted by real-time PCR and immunocytochemistry. The results of TCGA analysis showed that RhoC was overexpressed in OSCC tumor tissues. In vitro assays indicated that knockdown of RhoC did not have much effect on OSCC cell growth but significantly suppressed cell colony formation, invasion, and migration abilities, and F-actin polymerization was also reduced. The tongue-xenografted in vivo model demonstrated that knockdown of RhoC suppressed OSCC cell growth and inhibited metastasis to the superficial cervical lymph nodes. Further mechanism studies showed that knockdown of RhoC downregulated HMGA2 expression, and HMGA2 expression was highly correlated with RhoC expression in OSCC tumor tissues via the analysis of TCGA datasets. Overall, our study showed that knockdown of RhoC inhibited OSCC cells invasion and migration in vitro and OSCC cell growth and lymph node metastasis in vivo. Moreover, the potential mechanisms involved in these activities may be related to the regulation of HMGA2 expression. The RhoC gene could serve as a promising therapeutic target for OSCCs in the future.
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Liu, Zhi Han, Shao Teng Han, and Fang Chun Yang. "OSL: An Optimized Strategy of Data Replicas for Online Social Network." Applied Mechanics and Materials 263-266 (December 2012): 3230–33. http://dx.doi.org/10.4028/www.scientific.net/amm.263-266.3230.
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With the continued growth of social network users, nearly all the OSN (Online Social Network) providers replicate users’ data to different server nodes in order to ensure high reliability. Unlike traditional web applications, OSN represents a different class of data system that most of the data is based on friend relationship. In this paper, we propose OSL (Online Social Locality) algorithm, an online optimized strategy of data replicas based on social locality. This algorithm can decide the server node the replicas should be saved, and it improves system performance significantly.
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Pyun, Bo-Jeong, Young Sook Kim, Ik Soo Lee, Dong Ho Jung, Joo-Hwan Kim, and Jin Sook Kim. "Osteomeles schwerinae Extract and Its Major Compounds Inhibit Methylglyoxal-Induced Apoptosis in Human Retinal Pigment Epithelial Cells." Molecules 25, no. 11 (June 3, 2020): 2605. http://dx.doi.org/10.3390/molecules25112605.
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The accumulation and formation of advanced glycation end products (AGEs) are related to diabetes and age-related disease. Osteomeles schwerinae C. K. Schneid. (Rosaceae, OSSC) is used traditionally for the treatment of various diseases in Asia. Previous studies have shown that OSSC elicits preventive effects in an in vivo model of diabetes. This study was to evaluate the antiapoptotic effects of dried leaves and twigs of OSSC extract and its major compounds in ARPE-19 cells—spontaneously arising human retinal pigment epithelial cells—under diabetic conditions. To examine the effects of an OSSC extract and its active compounds (acetylvitexin, hyperoside and quercitrin) on apoptosis in methylglyoxal (MG, the active precursor in the formation of AGEs)-treated ARPE-19 cells and the mechanism by which these effects occur, apoptosis was measured using flow cytometry analysis. Protein expression levels of phospho-p53 (p-p53), Bax and Bcl-2 were determined by western blot analyses. The OSSC extract inhibited apoptosis in MG-treated ARPE-19 cells in a dose-dependent manner. The major compounds also reduced the rate of apoptosis. Both the extract and major compounds also inhibited the expression of p-p53 and Bax and increased the levels of Bcl-2 that had been previously reduced by MG treatment. The OSSC extract (0.1 μg/mL) and its major compounds (0.01 μM) attenuated apoptosis in ARPE-19 cells under toxic diabetic conditions by downregulating of expression of p-p53 and Bax. OSSC may serve as an alternative therapy to retard the development of diabetic retinopathy.
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Lee, Yi-Ju, Lung Chan, Chung-Min Yeh, Chien-Hsun Lee, and Wen-Wei Sung. "Overexpression of KLF17 Predicts a Favorable Prognosis in Patients with Oral Squamous Cell Carcinoma: A Retrospective Study." Medicina 56, no. 2 (January 30, 2020): 57. http://dx.doi.org/10.3390/medicina56020057.
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Background and Objectives: Patients with oral squamous cell carcinoma (OSCC), a common malignancy in Asian countries, have a poor prognosis. We investigated the role of Krüppel-like factor 17 (KLF17) and its prognostic significance in OSCC. Materials and Methods: KLF17 expression was measured by immunohistochemical staining of specimens from 283 patients with OSCC. We analyzed correlations between KLF17 expression and clinicopathologic features and between KLF17 expression and overall survival. The prognostic value of KLF17 was tested using Kaplan–Meier analysis and Cox proportional hazard models. Results: Among the 283 patients, high KLF17 expression was significantly associated with an early OSCC stage and low T-value (p = 0.033 and p = 0.036, respectively). The five-year survival rates were better in patients with high KLF17 expression than with low expression (66.5% and 49.6%, respectively). The prognostic role of KLF17 was further confirmed through multivariate analysis (hazard ratio 1.506, 95% confidence interval 1.034–2.191, p = 0.033). The prognostic value was more significant in patients with a history of betel quid chewing or with a low T-value. Conclusions: High KLF17 expression can serve as a marker for a favorable prognosis in patients with OSCC. The prognostic role of KLF17 is more significant in patients with a history of betel quid chewing or a low T-value.
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Nakamura, Kodai, Naomi Hiyake, Tomofumi Hamada, Seiya Yokoyama, Kazuki Mori, Kouta Yamashiro, Mahiro Beppu, Yasuaki Sagara, Yoshiaki Sagara, and Tsuyoshi Sugiura. "Circulating microRNA Panel as a Potential Novel Biomarker for Oral Squamous Cell Carcinoma Diagnosis." Cancers 13, no. 3 (January 25, 2021): 449. http://dx.doi.org/10.3390/cancers13030449.
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A lack of reliable biomarkers for oral squamous cell carcinoma (OSCC) poses a major clinical issue. The sensitivity and specificity of classical serum tumor markers, such as the squamous cell carcinoma antigen (SCC-Ag), are quite poor, especially for early detection. This study aimed to identify specific serum miRNAs potentially serving as OSCC biomarkers. The expression levels of candidate miRNAs in serum samples from 40 OSCC patients and 40 healthy controls were quantitatively analyzed via microarray and reverse transcription PCR (RT-PCR) analyses. To enhance the accuracy of detection, we used Fisher’s linear discriminant analysis to establish a diagnostic model that incorporated a combination of selected miRNAs. Consequently, miR-19a and miR-20a were significantly upregulated in the patient group (p = 0.014 and 0.036, respectively), whereas miR-5100 was downregulated (p = 0.001). We found that a combination of six miRNAs (miR-24, miR-20a, miR-122, miR-150, miR-4419a, and miR-5100) could distinguish between OSCC and the control group with a higher degree of accuracy (Area Under the Curve, AUC: 0.844, sensitivity: 55%, and specificity: 92.5%). Furthermore, compared to serum SCC antigen, the 6-miRNA panel could accurately detect the presence of OSCC. The present specific miRNAs panel may serve as a novel candidate biomarker of oral cancer.
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Ellis, Marc H. "The Future of Israel/Palestine: Embracing the Broken Middle." Journal of Palestine Studies 26, no. 3 (1997): 56–66. http://dx.doi.org/10.2307/2538157.
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This essay argues that the limitations and injustices of the Oslo agreement force a rethinking of the future of Israel/Palestine. The "separation" embodied in Oslo allows Jews to see the Israeli state as innocent and Palestinians to yearn for empowerment, but over the past hundred years a common history has been forged. The author argues that the disappointments on both sides constitute a "broken middle" that could serve as a common ground on which to build a shared future. The article ends with a plea for binationalism as the way to justice and reconciliation, arguing on historical, practical, and especially ethical grounds.
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Crowe, Ruth, Yasmine Probst, Jennifer Norman, Susan Furber, Lisa Franco, Rebecca M. Stanley, Cecilia Vuong, et al. "Healthy eating and physical activity environments in out-of-school hours care: an observational study protocol." BMJ Open 10, no. 9 (September 2020): e036397. http://dx.doi.org/10.1136/bmjopen-2019-036397.
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IntroductionChildcare settings have been widely identified as important venues for promoting healthy lifestyles to children. Out-of-school hours care (OSHC) is a rapidly growing childcare service, yet there has been limited research reported on healthy eating and physical activity (HEPA) environments within the Australian OSHC setting. This research aims to describe the HEPA environments related to foods and beverages served, staff behaviours and child physical activity levels across two local health districts within New South Wales, Australia. This study will provide evidence to support future interventions and policies in Australian OSHC settings.Methods and analysisA cross-sectional study design will be used to describe the food and beverages provided and child activity levels, and report on environmental correlates. OSHC programmes will be visited on non-consecutive weekdays between 2018 and 2020. The frequency of foods and beverages offered will be observed and categorised into food groups aligned to the Australian Dietary Guidelines. Children’s physical activity will be measured using ActiGraph wGT3X-BT accelerometers. Staff behaviour will be captured via direct observation and the System for Observing Staff Promotion of Activity and Nutrition. Short interviews with programme directors will gather contextual information about OSHC practices and policies.Ethics and disseminationFindings will be disseminated through peer-reviewed scientific journals, conference presentations and individualised feedback to each participating service. Ethical approval was granted by the University of Wollongong Human Research Ethics Committee (HE17/490).
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Lin, Yueh-Min, Mei-Ling Chen, Chia-Lo Chen, Chung-Min Yeh, and Wen-Wei Sung. "Overexpression of EIF5A2 Predicts Poor Prognosis in Patients with Oral Squamous Cell Carcinoma." Diagnostics 10, no. 7 (June 27, 2020): 436. http://dx.doi.org/10.3390/diagnostics10070436.
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Oral squamous cell carcinoma (OSCC) is the most common epithelial malignancy affecting the oral cavity, and it is especially significant in Asian countries. Patients diagnosed with OSCC have an unfavorable prognosis and additional prognostic markers would help improve therapeutic strategies. We sought to investigate the association between eukaryotic translation initiation factor 5A2 (EIF5A2) and epithelial–mesenchymal transition (EMT) markers as well as the prognostic significance of EIF5A2 in OSCC. The expression of EIF5A2 and EMT markers was measured through the immunohistochemical staining of specimens from 272 patients with OSCC. In addition, the correlation between different clinicopathological factors and EIF5A2 expression was analyzed. The prognostic role of EIF5A2 was then analyzed via Kaplan–Meier analysis and Cox proportional hazard models. Among the 272 patients, high EIF5A2 expression was significantly associated with an advanced N value (p = 0.008). High tumor expression of EIF5A2 was prone to the expression of low E-cadherin and high beta-catenin (p = 0.046 and p = 0.020, respectively). Patients with high EIF5A2 expression had unfavorable five-year survival rates as compared with those with low expression (49.7% and 67.3%, respectively). The prognostic role of EIF5A2 was further confirmed through multivariate analysis (hazard ratio = 1.714, 95% confidence interval: 1.134–2.590, p = 0.011). High EIF5A2 expression is associated with an advanced N value and EMT markers and may serve as a marker for an unfavorable prognosis in patients with OSCC.
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Ferrari, Elena, Margherita E. Pezzi, Diana Cassi, Thelma A. Pertinhez, Alberto Spisni, and Marco Meleti. "Salivary Cytokines as Biomarkers for Oral Squamous Cell Carcinoma: A Systematic Review." International Journal of Molecular Sciences 22, no. 13 (June 24, 2021): 6795. http://dx.doi.org/10.3390/ijms22136795.
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The prognosis of patients with oral squamous carcinoma (OSCC) largely depends on the stage at diagnosis, the 5-year survival rate being approximately 30% for advanced tumors. Early diagnosis, including the detection of lesions at risk for malignant transformation, is crucial for limiting the need for extensive surgery and for improving disease-free survival. Saliva has gained popularity as a readily available source of biomarkers (including cytokines) useful for diagnosing specific oral and systemic conditions. Particularly, the close interaction between oral dysplastic/neoplastic cells and saliva makes such fluid an ideal candidate for the development of non-invasive and highly accurate diagnostic tests. The present review has been designed to answer the question: “Is there evidence to support the role of specific salivary cytokines in the diagnosis of OSCC?” We retrieved 27 observational studies satisfying the inclusion and exclusion criteria. Among the most frequent cytokines investigated as candidates for OSCC biomarkers, IL-6, IL-8, TNF-α are present at higher concentration in the saliva of OSCC patients than in healthy controls and may therefore serve as basis for the development of rapid tests for early diagnosis of oral cancer.
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Lasensky, Scott. "Paying For Peace: The Oslo Process and the Limits of American Foreign Aid." Middle East Journal 58, no. 2 (April 1, 2004): 210–34. http://dx.doi.org/10.3751/58.2.13.
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American foreign aid has been essential for both cementing and sustaining efforts to resolve the Arab-Israeli conflict since the 1970s. During the Oslo process, aid was designed primarily to build public constituencies to support the negotiations. However, aid quickly became a bandage for a deteriorating Palestinian economy weighed down by corruption, damaged by violence, and stifled by Israeli closures. Rather than serve its original purpose, aid became a crutch for an unsteady process that collapsed following the 2000 Camp David summit. Unlike in other Arab-Israeli negotiations, where aid has been more effective, the Oslo process highlights the limits of foreign aid as an instrument of statecraft.
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Kim, Yan S., Esther J. Luo, Matthew T. Chin, Shelley Leong, Sudhir S. Rajan, and Gabriel J. Escobar. "The utilization and impact of oncology supportive care clinics on patients with metastatic lung cancer." Journal of Clinical Oncology 35, no. 31_suppl (November 1, 2017): 123. http://dx.doi.org/10.1200/jco.2017.35.31_suppl.123.
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123 Background: The American Society of Clinical Oncology has endorsed early integration of oncology with palliative care. In 2012, Kaiser Permanente Northern California (KPNC) introduced Oncology Supportive Care Clinics (OSCCs). OSCCs are outpatient palliative care clinics designed to incorporate palliative care into standard oncology practice at the time of a patient's cancer diagnosis. This study examines the utilization and impact of OSCCs on patients with metastatic lung cancer. Methods: We identified adult patients with incidental stage IV lung cancer using the KPNC cancer registry from 2012-2015 and followed them for 12 months. We ascertained OSCC utilization using KPNC’s electronic health record. We examined the patterns of OSCC referral/utilization and compared survival, in-hospital death, and treatment received in patients who used and did not use OSCC services. Results: A total of 607 patients were included and 245 (40.4%) were referred to OSCC. The median time from diagnosis to referral was 48 days and ¼ were referred within 15 days. The majority (86%) of the patients referred were seen by OSCC. Only 22 patients (9.0%) refused to be seen. We found no large patient- or facility-level differences between patients referred and not referred to OSCC. At the end of follow up, 398 (65.6%) of the patients died. Patients seen by OSCC were less likely to die (59.2% vs. 68.9%, p < 0.02) at 1 year. Fewer patients who used OSCC died in the hospital compared to those who did not (26 or 12.3% vs. 67 or 16.9%); however, it did not achieve statistical significance (p = 0.13). Among patients who used OSCC, 166 (78.7%) received anti-cancer treatment, compared to 221 (55.8%) among those who did not (p < 0.001). Conclusions: Despite having a robust infrastructure and integrated system in place, over half of the patients diagnosed with metastatic lung cancer did not receive a referral. Future work is needed to understand the reasons why so many patients were not referred. Furthermore, our data showed that receiving OSCC services did not adversely impact survival and the receipt of anti-cancer treatment. These findings serve to reassure patients and providers that the addition of palliative care does not harm patients or limit their treatment.
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de Lima, Jefferson Muniz, Grégoire B. Morand, Carolina Carneiro Soares Macedo, Luciana Diesel, Michael P. Hier, Alex Mlynarek, Luiz P. Kowalski, Mariana Maschietto, Moulay A. Alaoui-Jamali, and Sabrina Daniela da Silva. "NDRG1 deficiency is associated with regional metastasis in oral cancer by inducing epithelial–mesenchymal transition." Carcinogenesis 41, no. 6 (February 29, 2020): 769–77. http://dx.doi.org/10.1093/carcin/bgaa017.
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Abstract Regional metastasis is the single most important prognostic factor in oral squamous cell carcinoma (OSCC). Abnormal expression of N-myc downstream-regulated genes (NDRGs) has been identified to occur in several tumor types and to predict poor prognosis. In OSCC, the clinical significance of deregulated NDRG expression has not been fully established. In this study, NDRG1 relevance was assessed at gene and protein levels in 100 OSCC patients followed up by at least 10 years. Survival outcome was analyzed using a multivariable analysis. Tumor progression and metastasis was investigated in preclinical model using oral cancer cell lines (HSC3 and SCC25) treated with epidermal growth factor (EGF) and orthotopic mouse model of metastatic murine OSCC (AT84). We identified NDRG1 expression levels to be significantly lower in patients with metastatic tumors compared with patients with local disease only (P = 0.001). NDRG1 expression was associated with MMP-2, -9, -10 (P = 0.022, P = 0.002, P = 0.042, respectively) and BCL2 (P = 0.035). NDRG1 lower expression was able to predict recurrence and metastasis (log-rank test, P = 0.001). In multivariable analysis, the expression of NDRG1 was an independent prognostic factor (Cox regression, P = 0.013). In invasive OSCC cells, NDRG1 expression is diminished in response to EGF and this was associated with a potent induction of epithelial–mesenchymal transition phenotype. This result was further confirmed in an orthotopic OSCC mouse model. Together, this data support that NDRG1 downregulation is a potential predictor of metastasis and approaches aimed at NDRG1 signaling rescue can serve as potential therapeutic strategy to prevent oral cancer progression to metastasis.
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Mahendra, Ashish, Balasundari Shreedhar, Mala Kamboj, Arun Singh, Abhishek Singh, Ashutosh Agrawal, Sachin Kumar, and Arpita Kabiraj. "Epidermal Growth Factor Receptor Protein: A Biological Marker for Oral Precancer and Cancer." Journal of Dental Surgery 2014 (February 9, 2014): 1–7. http://dx.doi.org/10.1155/2014/158709.
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Background. The aim of the present paper was to study the expression and overexpression of EGFR in oral leukoplakia and cancer and at the same time assess the expression of EGFR in various histological grades of oral leukoplakia and OSCC in the Indian subcontinent. Methods. The study was conducted with routine H&E and IHC staining on 40 archival tissues. Results. Positive EGFR staining was present in all the cases 100% (30/30) out of which 7 (46.7%) cases of OSCC showed >75% EGFR expression and 8 (53.3%) cases of oral leukoplakia showed 25% EGFR expression. A statistically significant correlation was found in OSCC, OL and controls. Conclusions. EGFR may represent a promising target for novel molecular cancer therapies. EGFR expression levels in the premalignant lesion appear to be a sensitive factor in predicting the neoplastic potential of dysplastic tissues. This suggests that EGFR may serve as a biological marker to identify high-risk subgroups and guide prophylactic therapy.
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Zhao, Si-Yu, Jun Wang, Shao-Bo Ouyang, Zi-Kun Huang, and Lan Liao. "Salivary Circular RNAs Hsa_Circ_0001874 and Hsa_Circ_0001971 as Novel Biomarkers for the Diagnosis of Oral Squamous Cell Carcinoma." Cellular Physiology and Biochemistry 47, no. 6 (2018): 2511–21. http://dx.doi.org/10.1159/000491624.
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Background/Aims: Recent studies have demonstrated that circular RNAs (circRNAs) can serve as potential molecular markers for disease diagnosis. However, little is known about their diagnostic potential for oral squamous cell carcinoma (OSCC). This study aimed to determine the expression of circRNAs in the saliva of OSCC patients to identify novel biomarkers for OSCC screening. Methods: Microarray screening of circRNA was performed to identify differentially expressed circRNAs in saliva from 3 OSCC patients compared with 3 healthy controls. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to validate the results, and the association between these confirmed salivary circRNAs and clinicopathological features was analyzed using the chi-squared test. A receiver operating characteristic (ROC) curve was constructed to evaluate the diagnostic value of the circRNAs identified. Preoperative expression and postoperative expression (1 month after the surgery) of hsa_circ_0001874 and hsa_circ_0001971 was also determined. Results: Our results indicated 12 upregulated and 20 downregulated circRNAs in the saliva from the OSCC patients compared with that from the healthy controls. Among the differentially expressed circRNAs, hsa_circ_0001874, hsa_circ_0001971, and hsa_circ_0008068 were upregulated and hsa_circ_0000140, hsa_circ_0002632, and hsa_circ_0008792 were downregulated in the OSCC group versus the healthy group. Clinical data indicated that salivary hsa_circ_0001874 was correlated with TNM stage (P=0.006) and tumor grade (P=0.023) and that hsa_circ_0001971 was correlated with TNM stage (P=0.019). The combination of hsa_circ_0001874 and hsa_circ_0001971 showed an area under the ROC curve of 0.922 (95% confidence interval, 0.883-0.961; P< 0.001). The risk score based on the combination of hsa_circ_0001874 and hsa_circ_0001971 also discriminated patients with OSCC from patients with oral leukoplakia (P< 0.001). Moreover, the expression levels of salivary hsa_circ_0001874 and hsa_circ_0001971 were clearly decreased in the postoperative samples compared with preoperative samples (P< 0.001). Conclusions: This is the first study to demonstrate the potential of salivary hsa_circ_0001874 and hsa_circ_0001971 as biomarkers for the diagnosis of OSCC.
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Buvik, Kristin, and Bergljot Baklien. "“Girls will be served until you have to carry them out”: Gendered serving practices in Oslo." Addiction Research & Theory 24, no. 1 (May 25, 2015): 17–24. http://dx.doi.org/10.3109/16066359.2015.1049536.
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Chang, Yi-Lin, Yu-Kan Hsu, Tsung-Fan Wu, Chieh-Ming Huang, Li-Yin Liou, Ya-Wen Chiu, Yu-Hsuan Hsiao, Fuh-Jinn Luo, and Ta-Chun Yuan. "Regulation of estrogen receptor α function in oral squamous cell carcinoma cells by FAK signaling." Endocrine-Related Cancer 21, no. 4 (May 13, 2014): 555–65. http://dx.doi.org/10.1530/erc-14-0102.
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Estrogen receptor α (ERA) is a DNA-binding transcription factor that plays an important role in the regulation of cell growth. Previous studies indicated that the expression of ERα in cell lines and tumors derived from oral squamous cell carcinoma (OSCC). The aim of this study was to examine the activity and function of ERα in OSCC cells and the mechanism underlying ERα activation. Immunochemical analyses in benign (n=11) and malignant (n=21) lesions of the oral cavity showed that ERα immunoreactivity was observed in 43% (9/21) of malignant lesions, whereas none of benign lesions showed ERα immunoreactivity. The ERα expression was also found in three OSCC cell lines and its transcriptional activity was correlated with cell growth. Addition of estradiol stimulated cell growth, whereas treatment of tamoxifen or knockdown of ERα expression caused reduced cell growth. Interestingly, the expression and activity of focal adhesion kinase (FAK) were associated with the phosphorylation of ERα at serine 118 in OSCC cells. Elevated expression of FAK in the slow-growing SCC25 cells caused increases in ERα phosphorylation, transcriptional activity, and cell growth rate, whereas knockdown of FAK expression in the rapid-growing OECM-1 cells led to reduced ERα phosphorylation and activity and retarded cell growth. Inhibition of the activity of protein kinase B (AKT), but not ERK, abolished FAK-promoted ERα phosphorylation. These results suggest that OSCC cells expressed functional ERα, whose activity can be enhanced by FAK/AKT signaling, and this was critical for promoting cell growth. Thus, FAK and ERα can serve as the therapeutic targets for the treatment of OSCC.
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Senghore, Thomas, Wen-Chang Wang, Huei-Tzu Chien, You-Xin Chen, Chi-Kuang Young, Shiang-Fu Huang, and Chih-Ching Yeh. "Polymorphisms of Mismatch Repair Pathway Genes Predict Clinical Outcomes in Oral Squamous Cell Carcinoma Patients Receiving Adjuvant Concurrent Chemoradiotherapy." Cancers 11, no. 5 (April 29, 2019): 598. http://dx.doi.org/10.3390/cancers11050598.
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Background: We aimed to investigate the association between single-nucleotide polymorphisms (SNP) in mismatch repair (MMR) pathway genes and survival in patients with oral squamous cell carcinoma (OSCC) who received adjuvant concurrent chemoradiotherapy (CCRT). Methods: Using the Sequenom iPLEX MassARRAY system, five SNPs in four major MMR genes were genotyped in 319 patients with OSCC who received CCRT treatment. Kaplan–Meier survival curves and Cox proportional hazard regression models were used to assess overall survival (OS) and disease-free survival (DFS) among MMR genotypes. Results: The results of Kaplan–Meier survival analysis revealed that the MutS homolog 2 (MSH2) rs3732183 polymorphism showed a borderline significant association with DFS (log-rank p = 0.089). Participants with the MSH2 rs3732183 GG genotype exhibited a relatively low risk of recurrence (hazard ratio (HR) = 0.45; 95% confidence interval (CI) = 0.22–0.96; p = 0.039). In addition, the MutL homolog 1 (MLH1) rs1800734 GG genotype carriers exhibited higher OS (HR = 0.52, 95% CI = 0.27–1.01; p = 0.054) and DFS (HR = 0.49, 95% CI = 0.26–0.92; p = 0.028) rates. Conclusions: Our results indicated that the GG genotypes of MSH2 rs3732183 and MLH1 rs1800734 are associated with relatively high survival in OSCC patients treated using adjuvant CCRT. These polymorphisms may serve as prognosis predictors in OSCC patients.
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Liu, Juan, Xinjie Lian, Feng Liu, Xueling Yan, Chunyan Cheng, Lijia Cheng, Xiaolin Sun, and Zheng Shi. "Identification of Novel Key Targets and Candidate Drugs in Oral Squamous Cell Carcinoma." Current Bioinformatics 15, no. 4 (June 11, 2020): 328–37. http://dx.doi.org/10.2174/1574893614666191127101836.
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Background: Oral Squamous Cell Carcinoma (OSCC) is the most common malignant epithelial neoplasm. It is located within the top 10 ranking incidence of cancers with a poor prognosis and low survival rates. New breakthroughs of therapeutic strategies are therefore needed to improve the survival rate of OSCC harboring patients. Objective: Since targeted therapy is considered as the most promising therapeutic strategies in cancer, it is of great significance to identify novel targets and drugs for the treatment of OSCC. Methods: A series of bioinformatics approaches were launched to identify the hub proteins and their potential agents. Microarray analysis and several online functional activity network analysis were firstly utilized to recognize drug targets in OSCC. Subsequently, molecular docking was used to screen their potential drugs from the specs chemistry database. At the same time, the assessment of ligand-based virtual screening model was also evaluated. Results: In this study, two microarray data (GSE31056, GSE23558) were firstly selected and analyzed to get consensus candidate genes including 681 candidate genes. Additionally, we selected 33 candidate genes based on whether they belong to the kinases and transcription factors and further clustered candidate hub targets based on functions and signaling pathways with significant enrichment analysis by using DAVID and STRING online databases. Then, core PPI network was then identified and we manually selected GRB2 and IGF1 as the key drug targets according to the network analysis and previous references. Lastly, virtual screening was performed to identify potential small molecules which could target these two targets, and such small molecules can serve as the promising candidate agents for future drug development. Conclusion: In summary, our study might provide novel insights for understanding of the underlying molecular events of OSCC, and our discovered candidate targets and candidate agents could be used as the promising therapeutic strategies for the treatment of OSCC.
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Zhao, Zhen, Yan Xing, Fei Yang, Zhijun Zhao, Yupeng Shen, Junjian Song, and Shanghua Jing. "LncRNA HOXA-AS2 Promotes Oral Squamous Cell Proliferation, Migration, and Invasion via Upregulating EZH2 as an Oncogene." Technology in Cancer Research & Treatment 20 (January 2021): 153303382110391. http://dx.doi.org/10.1177/15330338211039109.
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Oral squamous cell carcinoma (OSCC) is one of the most common types of cancer worldwide. Accumulating evidence has shown that long noncoding RNAs (lncRNAs) serve important roles in the development of OSCC. The purpose of this study was to investigate the biological function and underlying regulatory mechanism of lncRNA homeobox A cluster antisense RNA2 (HOXA-AS2) in OSCC. RT-qPCR was performed to analyze the HOXA-AS2 expressions in human immortalized oral epithelial cell (HIOEC) line, human OSCC cell lines, and plasma. The expression of HOXA-AS2 and enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) in Tca-8113 cells were knocked down or overexpressed by transfection with shRNA-HOXA-AS2 or pcDNA-EZH2, respectively. The interaction between HOXA-AS2 and EZH2 was validated by RNA immunoprecipitation assay. In addition, cell proliferation was assessed by CCK-8 and EdU assays. Cell cycle distribution was analyzed by flow cytometry. Cell migration and invasion were detected using wound healing and Transwell assays, respectively. Apoptosis was detected by TUNEL staining. The protein expression levels of cell cycle and apoptosis-related proteins were measured by western blot analysis. Compared with HIOEC cells, HOXA-AS2 expression in OSCC cells was upregulated. HOXA-AS2 knockdown significantly inhibited Tca-8113 cell proliferation, blocked the cell cycle by arresting cells in the G0/G1 phase, promoted apoptosis, and suppressed migration and invasion. In addition, HOXA-AS2 was predicted to directly target EZH2 and positively regulate EZH2 expression. EZH2 overexpression could reverse the inhibitory effect of HOXA-AS2 knockdown on the proliferation, migration, and invasion of Tca-8113 cells. In summary, the findings suggested that HOXA-AS2 may inhibit cell proliferation, invasion, and migration, induce cell cycle arrest in the G0/G1 phase, and increase cell apoptosis by targeting EZH2. The research indicated that HOXA-AS2/EZH2 axis may play a key role in the development of OSCC.
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Li, Baorong, Yingmiao Liu, Cong Zhang, Yan Kou, Lili Zou, Hui Liang, Li Hou, et al. "Neutrophil Extracellular Traps Enhance Procoagulant Activity in Patients with Oral Squamous Cell Carcinoma." Blood 132, Supplement 1 (November 29, 2018): 2399. http://dx.doi.org/10.1182/blood-2018-99-115737.
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Abstract Background: Cancer patients are considered to be prothrombotic with major disturbances in hemostasis that are associated with an increased risk of venous thromboembolism, especially in patients with advanced cancer. Recently reports show that the high levels of circulating microparticles (MPs) have procoagulant activity (PCA) in oral squamous cell carcinoma (OSCC). However, this study did not address the question of what specific mechanism might underlie the PCA in OSCC. Neutrophil extracellular traps (NETs) are activated neutrophil-derived web-like structures, which have emerged as important mediators in cancer progression, metastasis and cancer-associated thrombosis. Additionally, the cytokines and neutrophils were known to become aggregated in cancers and are usually present in high numbers in OSCC patients and are associated with poor outcomes. The exact molecular mechanisms responsible for modulation of neutrophils procoagulant functions in OSCC are, however, poorly understood. Thus, we hypothesized that cytokines might activate neutrophils to release NETs, thereby predisposing OSCC patients to a hypercoagulative state. Moreover, we evaluated NETs interaction with human umbilical vein endothelial cells (HUVECs) and their association with pathological lesions in this disease. Methods: OSCC patients (n = 58) were divided into four stages according to the 2009 guidelines of the American Joint Committee on Cancer staging classification, and compared to healthy controls (n = 25). Cell-free DNA (cf-DNA) was quantified using the Quant-iT PicoGreen dsDNA Assay Kit. ELISA was used to detect MPO-DNA complexes, TAT (thrombin-antithrombin) complexes, neutrophil elastase, nucleosomes, and cytokines. PCA of NETs was evaluated using coagulation time and purified coagulation complex and fibrin production assays. Phosphatidylserine (PS) exposure, fibrin strands, and FVa/Xa binding on cells were observed using confocal microscopy. Results:Plasma levels of NET markers in patients with stage III/IV OSCC were significantly higher than those in stage I/II patients or controls (all p<0.05), and positively correlated with thrombin-antithrombin (TAT) complex and fibrinogen levels. Interestingly, neutrophils from OSCC patients with stage III/IV were more prone to release NETs compared to those from stage I/II patients and controls. Additionally, we found that plasma from patients with stage III/IV OSCC was able to prime neutrophils to generate higher amounts of NETs than from stage I/II patients and controls. Depleting IL-8, IL-6 and TNF-a reduced plasma-enhance NETs release. In addition, NETs released by stage III/IV OSCC neutrophils significantly increased the potency of control plasma to generate thrombin and fibrin, greatly shortened the coagulation time (all p<0.05). These effects were attenuated by DNase I. Finally, isolated NETs induced ECs to lose normal morphology and retract from their cell-cell junctions, converting them to a pro-coagulant phenotype. DNase I attenuated this cytotoxicity. Conclusion s :These results suggest that OSCC creates a systemic inflammation environment that primes neutrophils to release procoagulant NETs in patients with stage III/IV OSCC. The NETs formation correlated positively with the parameters of disease severity. The information that results from these investigations may serve as a rational basis for the design of future drug intervention trials that target coagulation reactions, mechanisms and/or interactions relevant to OSCC. Disclosures No relevant conflicts of interest to declare.
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Arnold, Melina, Jacques Ferlay, Mark I. van Berge Henegouwen, and Isabelle Soerjomataram. "Global burden of oesophageal and gastric cancer by histology and subsite in 2018." Gut 69, no. 9 (June 30, 2020): 1564–71. http://dx.doi.org/10.1136/gutjnl-2020-321600.
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ObjectivesTo provide updated estimates of the global burden of oesophageal and gastric cancer by subsite and type.MethodsUsing data from population-based cancer registries, proportions of oesophageal adenocarcinoma (OAC) and squamous cell carcinoma (OSCC) out of all oesophageal as well as cardia gastric cancer (CGC) and non-CGC (NCGC) out of all gastric cancer cases were computed by country, sex and age group. Proportions were subsequently applied to the estimated numbers of oesophageal and gastric cancer cases from GLOBOCAN 2018. Age-standardised incidence rates (ASR) were calculated.ResultsIn 2018, there were an estimated 572 000 new cases of oesophageal cancer worldwide, 85 000 OACs (ASR 0.9 per 100 000, both sexes combined) and 482 000 OSCCs (ASR 5.3). Out of 1.03 million gastric cancers, there were an estimated 181 000 cases of CGC (ASR 2.0) and 853 000 cases of NCGC (ASR 9.2). While the highest incidence rates of OSCC, CGC and NCGC were observed in Eastern Asia (ASRs 11.1, 4.4 and 17.9, respectively), rates of OAC were highest in Northern Europe (ASR 3.5). While globally OSCC and NCGC remain the most common types of oesophageal and gastric cancer, respectively, rates of OAC exceed those of OSCC in an increasing number of high-income countries.ConclusionsThese updated estimates of the global burden of oesophageal and gastric cancer by subtype and site suggest an ongoing transition in epidemiological patterns. This work will serve as a cornerstone for policy-making and will aid in developing appropriate cancer control strategies.
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Gusmao Brissi, Sara, Luciana Debs, and Emad Elwakil. "A Review on the Factors Affecting the Use of Offsite Construction in Multifamily Housing in the United States." Buildings 11, no. 1 (December 24, 2020): 5. http://dx.doi.org/10.3390/buildings11010005.
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The increasing demand for multifamily housing in the United States requires alternatives for building more affordable and sustainable housing to improve the quality of life for millions of families. Offsite construction (OSC) strategies may be a viable alternative for tackling this problem. Although the use of OSC is significant in the multifamily housing market in the world and it is also very promising in the US, a scarce amount of literature has focused on this topic. The purpose of this study is to identify specific factors that affect decisions on the use of OSC in multifamily housing in the US. Focusing on the sustainability dimensions of construction—social, environmental, and economic—the authors reviewed literature that was published between 2000 and 2019 and identified factors that are related to OSC adoption in general construction, in housing construction, and, more specifically, in multifamily housing construction in the US. Subsequently, a discussion on some important factors affecting decisions on the use of OSC in the American multifamily market is provided. The discussion focused on factors that, although important, have been under explored in the literature that addresses the use of OSC in multifamily projects, especially in the US, which are: customer’s attitude, building performance, and building comfort and indoor environmental quality (IEQ). In addition, a brief discussion regarding the importance of design to the uptake of OSC in multifamily projects is provided. This is one of the first studies dedicated to exploring the social, environmental, and economic factors that affect the use of OSC in multifamily housing in the US. The study also identifies research gaps, which serve as a roadmap for future research.
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Al-Kaabi, A., L. W. van Bockel, A. J. Pothen, and S. M. Willems. "p16INK4Aand p14ARFGene Promoter Hypermethylation as Prognostic Biomarker in Oral and Oropharyngeal Squamous Cell Carcinoma: A Review." Disease Markers 2014 (2014): 1–8. http://dx.doi.org/10.1155/2014/260549.
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Head and neck squamous cell carcinoma is a heterogeneous group of tumors with each subtype having a distinct histopathological and molecular profile. Most tumors share, to some extent, the same multistep carcinogenic pathways, which include a wide variety of genetic and epigenetic changes. Epigenetic alterations represent all changes in gene expression patterns that do not alter the actual DNA sequence. Recently, it has become clear that silencing of cancer related genes is not exclusively a result of genetic changes such as mutations or deletions, but it can also be regulated on epigenetic level, mostly by means of gene promoter hypermethylation. Results from recent studies have demonstrated that DNA methylation patterns contain tumor-type-specific signatures, which could serve as biomarkers for clinical outcome in the near future. The topic of this review discusses gene promoter hypermethylation in oral and oropharyngeal squamous cell carcinoma (OSCC). The main objective is to analyse the available data on gene promoter hypermethylation of the cell cycle regulatory proteinsp16INK4Aandp14ARFand to investigate their clinical significance as novel biomarkers in OSCC. Hypermethylation of both genes seems to possess predictive properties for several clinicopathological outcomes. We conclude that the methylation status ofp16INK4Ais definitely a promising candidate biomarker for predicting clinical outcome of OSCC, especially for recurrence-free survival.
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Tsodoulos, L. M., K. Stamoulis, C. A. Papachristodoulou, K. G. Ioannides, and S. Pavlides. "Use of the Optically Stimulated Luminescence Dating and X-Ray Fluorescence Spectrometry Methods as Tools in Paleoseismology." HNPS Proceedings 22 (March 8, 2019): 107. http://dx.doi.org/10.12681/hnps.1940.
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We have investigated the application of luminescence dating to sediment and pottery samples from a paleoseismological trench excavated in the Gyrtoni Fault, Tyrnavos Basin, Central Greece. The samples were dated following the optically stimulated luminescence (OSL) dating method, using the Riso TL/OSL DA-20 reader. The OSL ages were obtained from chemically purified quartz and a single-aliquot regenerative-dose (SAR) protocol was followed for the equivalent dose (De) determination. Additionally, samples were collected and analyzed with the method of X-ray Fluorescence (XRF) spectrometry, in order to assess their elemental composition. Radioisotope sources (109Cd and 241Am) were used for sample excitation, while X-ray spectra were acquired using a Si(Li) detector coupled with standard electronics. The XRF data were submitted to principal component analysis (PCA). This statistical handling aimed to distinguish from which part of the upthrown fault block scarp-derived colluvium and alluvial deposits, parts of the downthrown block were derived and thus estimate the displacement. The results indicated that both the OSL dating method and the XRF analysis combined with PCA can serve as useful tools for paleoseismological investigations.
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Marzukhi, Marlyana Azyyati, Dasimah Omar, Oliver Ling Hoon Leh, Na’asah Nasrudin, and Azfarnizam Jaafar. "Enhancing One Stop Centre in the Malaysian Planning System." E3S Web of Conferences 101 (2019): 01001. http://dx.doi.org/10.1051/e3sconf/201910101001.
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Malaysia has been experiencing rapid development since its independence in 1957, which has transformed its economic base from agriculture to industry. Rapid urbanisation has itself led to the continued rise of economic growth and the need for obtaining permissions from the relevant authorities to ensure an effective and efficient planning system. This effort is evidenced by the improvement of mechanism delivery system of planning and building plan process, known as One Stop Centre (OSC). The Ministry of Urban Wellbeing, Housing and Local Government initiated OSC on the 13th April 2007 to improve the planning delivery system and procedures at all local planning authorities by coordinating and shortening the approval process. However, relatively little is known about the effectiveness of OSC and the understanding of its roles among the stakeholders in the local authority. Therefore, a questionnaires survey has been conducted to forty-seven (47) respondents and interviews with the public that involved in the process. The respondents have mostly felt that the ineffectiveness of the planning and building plan approval process was due to the incomplete documents submitted to the OSC, lack of knowledge among the Professional Submitting Person (PSP) and the incapability of staffs in handling development applications. Hence, the findings present a synthesis of results for town planners, architects, developers and government agencies to have a better understanding of OSC. Thus, the knowledge serves as a basis for future strategic planning decisions and guidance in the delivery system in Malaysia.
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Maturana-Espinosa, José Carmelo, Juan Pablo García-Ortiz, Daniel Müller, and Vicente González-Ruiz. "Layer Selection in Progressive Transmission of Motion-Compensated JPEG2000 Video." Electronics 8, no. 9 (September 13, 2019): 1032. http://dx.doi.org/10.3390/electronics8091032.
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MCJ2K (Motion-Compensated JPEG2000) is a video codec based on MCTF (Motion- Compensated Temporal Filtering) and J2K (JPEG2000). MCTF analyzes a sequence of images, generating a collection of temporal sub-bands, which are compressed with J2K. The R/D (Rate-Distortion) performance in MCJ2K is better than the MJ2K (Motion JPEG2000) extension, especially if there is a high level of temporal redundancy. MCJ2K codestreams can be served by standard JPIP (J2K Interactive Protocol) servers, thanks to the use of only J2K standard file formats. In bandwidth-constrained scenarios, an important issue in MCJ2K is determining the amount of data of each temporal sub-band that must be transmitted to maximize the quality of the reconstructions at the client side. To solve this problem, we have proposed two rate-allocation algorithms which provide reconstructions that are progressive in quality. The first, OSLA (Optimized Sub-band Layers Allocation), determines the best progression of quality layers, but is computationally expensive. The second, ESLA (Estimated-Slope sub-band Layers Allocation), is sub-optimal in most cases, but much faster and more convenient for real-time streaming scenarios. An experimental comparison shows that even when a straightforward motion compensation scheme is used, the R/D performance of MCJ2K competitive is compared not only to MJ2K, but also with respect to other standard scalable video codecs.
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Mauramo, Matti, Tuulia Onali, Wafa Wahbi, Jenni Vasara, Anniina Lampinen, Elina Mauramo, Anne Kivimäki, et al. "Bilberry (Vaccinium myrtillus L.) Powder Has Anticarcinogenic Effects on Oral Carcinoma In Vitro and In Vivo." Antioxidants 10, no. 8 (August 22, 2021): 1319. http://dx.doi.org/10.3390/antiox10081319.
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Previous studies indicate that bilberry with high amounts of phenolic compounds can inhibit carcinogenic processes of colorectal cancer in vitro and in vivo. However, no studies have focused on the effects of bilberry on oral cancer. In this study, we aimed to examine the effects of bilberry powder on oral squamous cell carcinoma (OSCC) cells using both in vitro and in vivo assays. The effects of 0, 1, 10, and 25 mg/mL of whole bilberry powder on the viability, proliferation, migration, and invasion of OSCC (HSC-3) cells were examined and compared with 0.01 mg/mL of cetuximab. Two oral keratinocyte cell lines served as controls. Tumor area was analyzed in zebrafish microinjected with HSC-3 cells and treated with 2.5, 10, or 25 µg/mL of bilberry powder. Metastases in the head or tail areas were counted. Bilberry powder inhibited the viability, proliferation, migration, and invasion of HSC-3 cells (p < 0.05), which was more pronounced with higher concentrations. Cetuximab had no effect on HSC-3 cell migration or invasion. Compared to controls, the tumor area in zebrafish treated with bilberry powder (10 and 25 µg/mL) was reduced significantly (p = 0.038 and p = 0.021, respectively), but the number of fish with metastases did not differ between groups. Based on our in vitro and in vivo experiments, we conclude that whole bilberry powder has anti-tumor effects on OSCC cells.