Dissertations / Theses on the topic 'Organ culture'
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Heil, Bradley R. "DISTRACTION OSTEOGENESIS IN AN ORGAN CULTURE MODEL." UKnowledge, 2010. http://uknowledge.uky.edu/gradschool_theses/47.
Full textRoach, H. I. "Possibilities and limitations of bone organ culture." Thesis, University of Southampton, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.377420.
Full textWortman, Morris Rachel. "Facing the Waitlist: Visual Grammars of Organ Donation and Transplantation." The Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1338035019.
Full textBerggren, Diana. "Applications of organ culture of the mouse inner ear." Doctoral thesis, Umeå universitet, Öron- näs- och halssjukdomar, 1991. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-99333.
Full textS. 1-34: sammanfattning, s. 37-88: Härtill 6 uppsatser
digitalisering@umu
Finney, K. J. "Effects of trophic factors on intestinal mucosa in organ-culture." Thesis, University of Newcastle Upon Tyne, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.382882.
Full textSMITH, PETER FRANCIS. "A NOVEL ORGAN CULTURE SYSTEM FOR THE STUDY OF HEPATOTOXICITY)." Diss., The University of Arizona, 1985. http://hdl.handle.net/10150/188134.
Full textColetta, Riccardo. "Manipulating growth and differentiation of embryonic intestine in organ culture." Thesis, University of Manchester, 2017. https://www.research.manchester.ac.uk/portal/en/theses/manipulating-growth-and-differentiation-of-embryonic-intestine-in-organ-culture(ad43b8a7-2499-4813-a1de-c46a24a079aa).html.
Full textConklin, Brian Scott. "Viability of porcine common carotid arteries in a novel organ culture system." Thesis, Georgia Institute of Technology, 1997. http://hdl.handle.net/1853/17282.
Full textShidrawi, Ray Georges. "Molecular immune aspects of coeliac disease : organ culture and peptide binding studies." Thesis, King's College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.243337.
Full textWatts, Susan Margaret. "Inhibition of neointima formation using the human saphenous vein organ culture model." Thesis, University of Bristol, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.264064.
Full textLin, Yi-Cheng. "Novel organ culture model for a complete synovial joint : creation and application." Thesis, University of Edinburgh, 2015. http://hdl.handle.net/1842/15850.
Full textSöderpalm, Annika. "Photoreceptor development and degeneration in retinal organ culture Effects of neurotrophic factors /." [S.l. : s.n.], 1999. http://catalog.hathitrust.org/api/volumes/oclc/44519577.html.
Full textTronshaug, Hans Jacob Høyem. ""With rare diligence and accuracy" : the organ building of Peter Adolph Albrechtsen in the context of nineteenth-century Danish/Norvegian organ culture /." Göteborg : Göteborgs Universitet, 2001. http://catalogue.bnf.fr/ark:/12148/cb390003593.
Full textDavis, Nathan Peter. "Axial stretch as a means of lengthening arteries : an investigation in organ culture." Diss., Georgia Institute of Technology, 2002. http://hdl.handle.net/1853/17645.
Full textAnderton, T. "Pathogenesis of Bordetella bronchiseptica infection in canine respiratory tract air interface organ culture." Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.596103.
Full textNunes, Sandro Filipe Fernandes. "Influenza A infection dynamics in an Ex vivo organ culture of pig trachea." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609311.
Full textSasaki, Kaori. "Politicised culture, culturalised life and death : the Japanese organ transplantation and brain death debates." Thesis, Lancaster University, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.441371.
Full textLeung, Theresa. "Cellular and tissue responses to implant materials : development of a novel organ culture model." Thesis, University College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.285839.
Full textPalmer, Marion Elizabeth. "The significance of oxidative damage in the organ culture of early placental chorionic villi." Thesis, University of Cambridge, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.627203.
Full textGazes, Seth Brian. "Computer controlled device to independently control flow waveform parameters during organ culture and biomechanical testing of mouse carotid arteries." Thesis, Atlanta, Ga. : Georgia Institute of Technology, 2009. http://hdl.handle.net/1853/31812.
Full textCommittee Chair: Rudy Gleason; Committee Member: Raymond Vito; Committee Member: W. Robert Taylor. Part of the SMARTech Electronic Thesis and Dissertation Collection.
Wu, Xiaohong. "Development of a mouse calvarial defect model for evaluation of biomaterials : an organ culture study." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.506280.
Full textChan, Kit-ying, and 陳潔瑩. "Development of whole disc organ culture system and acellular disc scaffold for intervertebral disc engineering." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45600077.
Full textLall, Sonia. "Studies in organ culture and the development of organogenic potential in Alnus, Sorbus and Prunus." Thesis, University of East London, 2000. http://roar.uel.ac.uk/3586/.
Full textOkano, Junko. "Transforming growth factor beta2 promotes the formation of the mouse cochleovestibular ganglion in organ culture." Kyoto University, 2006. http://hdl.handle.net/2433/143848.
Full textLee, Bee Eng Adeline Media Film & Theatre UNSW. "Organ donation and anti-littering campaigns: a comparative study of Australia and Singapore." Awarded by:University of New South Wales. Media, Film and Theatre, 2005. http://handle.unsw.edu.au/1959.4/27211.
Full textAndersson, Patric, Jeanette Henrysson, and Katarina Johannson. "Att fatta beslut i organdonationsfrågan – Vad påverkar?" Thesis, Halmstad University, School of Social and Health Sciences (HOS), 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:hh:diva-560.
Full textThere is a shortage of organs available for donation worldwide. There have been several campaigns to try to increase the numbers of registrered donors. The topic has been brought to the forefront but more work is still needed to distribute information to the public.
The aim of this literature review was to examine what influences people in their decision to donate their organs or not, and what role nurses play in providing the most current information available. The study found that there was several different reasons given for not donating their organs. A great number of people were convinced that their religion was against organ donation. The level of knowledge about organ donation and ethnoculural background were major factors when making the decision to register as an organ donar. Only through making more information available in different languages, educating healthcare workers about culural and religions differences can the numbers of registrated donors increase.
Hashemi, Elham. "Precision-cut liver slices as a system for studying xenobiotic metabolism." Thesis, University of Surrey, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.326474.
Full textUmez-Eronini, Oparaku Nkem. "Evaluation of polyHIPE as a scaffold for bladder tissue engineering in a three dimensional organ culture model." Thesis, University of Newcastle upon Tyne, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419990.
Full textTaura, Akiko. "Recovery of hair cell function after damage induced by gentamicin in organ culture of rat vestibular maculae." Kyoto University, 2007. http://hdl.handle.net/2433/135651.
Full textZhang, Pu. "Human ovarian follicles and oocytes : collection, cryopreservation, culture and gene expression /." Stockholm, 2005. http://diss.kib.ki.se/2005/91-7140-281-0/.
Full textPaoli, Roberto. "Cell culture interfaces for different organ-on-chip applications: from photolithography to rapid-prototyping techniques with sensor embedding." Doctoral thesis, Universitat de Barcelona, 2019. http://hdl.handle.net/10803/668376.
Full textEn los últimos años está emergiendo una nueva propuesta para mejorar los modelos actuales en el estudio de nuevos fármacos. Mediante la fusión de cultivos celulares y microfluídica ha nacido un nuevo campo de aplicación denominado “Órgano-en-un-chip” (OOC), donde se recrea un entorno fisiológico capaz de reproducir unidades funcionales mínimas de diversos órganos del cuerpo humano. Un elemento importante para el desarrollo de dispositivos OOC es la reproducción de zonas de interacción entre varios tejidos formados por diferentes tipos celulares. Esta tesis, titulada “Interfaces de cultivo celular para diferentes aplicaciones de OOC: desde fotolitografía a técnicas de prototipado rápido con inclusión de sensores”, tiene como objetivo el diseño, simulación y evaluación de dispositivos OOC capaces de reproducir superficies de contacto de tejidos contiguos expuestos a flujo. El trabajo está enfocado a la exploración de nuevas técnicas de fabricación que permitan el prototipado rápido de dispositivos OOC, reduciendo costes, tiempo y mano de obra asociada a dicha fabricación. El objetivo final es demostrar la utilidad de los dispositivos como herramientas de investigación para problemas biológicos, aplicándolos en esta tesis al estudio del túbulo renal y de la barrera hematoencefálica. Para ello se han fabricado tres versiones de dispositivos: 1) OOCv1 fabricado por litografía suave en múltiples capas de PDMS; 2) OOCv2 fabricado con cortadora de vinilo y cortadora láser en múltiples capas de materiales termoplásticos y con electrodos integrados en la versión OOCv2.2; 3) OOCv3 fabricado mediante impresión 3D por esterolitografía. Todos los dispositivos están hechos de materiales biocompatibles de alta calidad óptica, con conectores fluídicos y una membrana comercial integrada. Los experimentos biológicos sobre túbulo renal, realizados en los dispositivos OOCv1 y OOCv2, han demostrado la viabilidad de los dispositivos, integrados con un sistema de flujo, para estudios de la metabolización de ácidos grasos en el riñón relacionados con condiciones diabetogénicas. Los experimentos biológicos sobre la barrera hematoencefálica han confirmado la viabilidad de OOCv2 para el cocultivo compartimentado de células endoteliales de cerebro y pericitos. La integración de electrodos en el OOCv2.2 ha demostrado ser una técnica fiable para la medición de la integridad de barreras biológicas de modo no-invasivo, libre de etiqueta (“label-free”), y a tiempo real gracias a la espectroscopía de impedancia.
Essaouiba, Amal. "Development of a liver-pancreas in vitro model using microfluidic organ-on-chip technologies." Thesis, Compiègne, 2020. http://www.theses.fr/2020COMP2573.
Full textDiabetes mellitus (DM) or the so called disease of the century is a life threatening dysfunction that affects the endocrine system. The mechanisms underlying the break in the feedback loop that regulates the metabolism and the consequent diabetes induction are not fully known. Understanding the mechanisms of insulin action is therefore crucial for the further development of effective therapeutic strategies to combat DM. Accordingly, it is imperative to find a robust and reliable model for diabetes research able to overcome the limitations of traditional 2D in vitro cell culture and animal experimentation. The aim of this thesis is to develop a new liver‐pancreas co‐culture model using advanced microphysiological systems (MPs) to tackle more effectively the mechanism involving the hepatic and pancreatic endocrine regulation. This work highlights the power of multi organ‐on‐chip systems that combines the advanced 3D‐cell compartmentalization, microfluidics and induced pluripotent stem cells (iPSC) technology to achieve a high biological complexity and functions that are rarely reproduced by only one of these tissue engineering technologies
Putoczki, Tracy Lynn. "A study of the efficacy of organ cultures to examine wood formation in Pinus radiata D. Don." Thesis, University of Canterbury. Biological Sciences, 2006. http://hdl.handle.net/10092/917.
Full textCandito, Antonio. "Modelling, simulation and characterization of epithelial cell culture biochip." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8483/.
Full textSong, Miyeoun. "Organ and primary culture of medaka (Oryzias latipes) testis: Test systems for the analysis of cell proliferation and differentiation." Doctoral thesis, Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2003. http://nbn-resolving.de/urn:nbn:de:swb:14-1059039500031-89370.
Full textTsilingiri, Aikaterini. "A novel organ culture model for polarized stimulation of human intestinal mucosa : probiotics and postbiotics in health and disease." Thesis, University of Aberdeen, 2014. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203945.
Full textMiddlebrook, Aaron J. "Nicotine and TNF alpha, modulators of T cell signaling-effects on T cell development in fetal thymus organ culture." Diss., The University of Arizona, 2004. http://hdl.handle.net/10150/280628.
Full textKrammer, Thibault. "Développement d'un réseau microvasculaire sur puce microfluidique pour la reconstruction tissulaire." Thesis, Université Grenoble Alpes (ComUE), 2019. http://www.theses.fr/2019GREAV044.
Full textTissue engineering aims to develop functional tissues or organs in vitro in order to provide drug testing platforms or transplantable tissues and improve the treatments provided to patients. However, the physiological tissue structures developed to date do not integrate and perfusable vascular network. In vivo, the vascular network supplies the body’s cells with oxygen and nutrients and removes cellular waste and carbon dioxide. It also has a major role in maintaining organ homeostasis. Blood capillaries are hollow vessels whose walls are only composed of a layer of endothelial cells and diffuse nutrients. The blood capillary network is dense and perfuse all tissues. Due to the limit oxygen diffusion inside tissues, each cell is located at most 200µm away from a capillary. The difficulties of building a network of perfusable capillaries and integrating them into tissue constructs limit the development of thick physiological tissues.An innovative technique for developing a microvascular network within a thick construction is presented in this thesis. This technique consists of assembling spherical tissue micro-units within a microfluidic chamber, and developing a network of capillaries through the interstitial pores formed by the spheres packing. Tissue micro-units are composed of biopolymers representative of the extracellular matrix and contain cells from the tissue of interest. A layer of endothelial cells is developed on the surface of these microspheres. The stacking of these microspheres creates a porous medium in which nutrient medium is perfused. Flow control within such a structure allows the application of physical stimuli influencing the self-assembly of endothelial cells into capillaries within the interstitial space of the sphere packing.During this thesis, a device for manufacturing microspheres from natural biopolymers was developed. The structure formed by the stacks of spheres was studied and the flow within such environments were characterized so as to apply controlled physical stimuli to cells. A bioreactor-like perfusion system has been built. A thick tissue structure could be formed within this system and the development of the vascular network was promoted. The formation of the network was demonstrated by the presence of infused blood capillaries within the structure. The technique developed promises to be applied to the development of many tissues and applications for organ-on-chip or tissue engineering devices
Saarela, U. (Ulla). "Novel culture and organoid technologies to study mammalian kidney development." Doctoral thesis, Oulun yliopisto, 2018. http://urn.fi/urn:isbn:9789526218250.
Full textTiivistelmä Munuaissairauksiin sairastuvien määrä on lisääntynyt maailmanlaajuisesti, ja se on aikaansaanut tarpeen uusien hoitokeinojen sekä siirtoelimien kehitykseen. Näiden kehittämiseksi tarvitsemme uutta tietoa munuaisen kehityksestä ja toiminnasta. Munuaisen kehitystä on tutkittu ex vivo -viljelyn avulla jo yli vuosisadan ajan, mutta nykyiset elinviljelytekniikat eivät ole kuitenkaan optimaalisia pitkäkestoiseen time-lapse-kuvaukseen. Tässä työssä käytetään munuaisen kehityksen tutkimiseen hiiren alkion munuaisia sekä munuaisorganoideja, jotka ovat munuaissoluista koostuvia ja aitoa munuaista mallintavia soluaggregaatteja. Primaaristen munuaissolujen käyttöön sisältyy rajoitteita, ja tämä luo tarpeen uusien organoiditekniikoiden kehitykseen ja optimointiin. Primaarisia munuaissoluja on yleensä käytettävissä pieniä määriä, ja ne eivät myöskään sovellu pitkäkestoiseen kasvatukseen, koska ne menettävät nopeasti kykynsä muodostaa nefroneita. Uusien tekniikoiden avulla voidaan parantaa näiden solujen kasvatusta, säilytystä ja transfektointia ja edistää eri geenien vaikutuksia tutkivat funktionaaliset testaukset. Tässä tutkimuksessa esitetään uusia työkaluja nefrogeneesin tutkimiseen. Elinten kehitystä seuraavan time-lapse-kuvauksen laatua voidaan parantaa käyttämällä tässä työssä esitettyä FiZD-kasvatusmenetelmää, jossa munuaiseksplantti kasvaa rajoitetussa 70μm:n tilassa. Kuvat ovat korkealaatuisia, ja se mahdollistaa 2D-kuvan yksittäisten solujen segmentoinnin ja solujen liikkeiden dynaamisen analyysin. Lisäksi tässä tutkimuksessa esitetään ei-indusoidun munuaismesenkyymin käsittelyyn kehitetty dissosiaatio- ja reaggregaatiomenetelmä. Munuaisen kehityksen alkuvaiheessa on mahdollistaerottaa nefroneja muodostava metanefrinen mesenkyymi (MM) sekä munuaisen kokoajaputkiston muodostava ureterin silmu. Metanefrinen mesenkyymi voidaan hajottaa yksisolususpensioksi, säilyttää 24 tuntia kasvutekijämediumissa ja tämän jälkeen reaggregoida ja indusoida muodostamaan nefroneita. Tämä tekniikka mahdollistaa MM-solujen geneettisen muokkauksen, ennen kuin munuaisen kehitys alkaa. Tämä tekniikka mahdollistaa myös dissosioitujen MM solujen geneettiset muokkaukset. Geenien yliekspression tai hiljentämisen avulla voidaan tehdä funktionaalisia kokeita näiden muutosten vaikutuksesta nefrogeneesiin. Lisäksi tässä työssä esitetään munuaisprogenitorisolujen säilömistä syväjäädytyksellä. Munuaisprogenitorisolut voidaan säilöä nestetyppeen, minkä jälkeen ne ovat edelleen kykeneviä muodostamaan nefronirakenteita tai haarautumaan. Tässä väitöskirjatyössä esitettyjen menetelmien avulla on tulevaisuudessa mahdollista saada lisätietoa munuaisten kehitysprosessista. Kehitetty FiZD-kasvatusmenetelmä parantaa munuaisen kehityksen kuvantamista ja mahdollistaa yksittäisten solujen seuraamisen. Tämä kasvatusmenetelmä sopii myös muiden elinten, kuten munarauhasten, ja kudosten kasvatukseen, ja sen avulla voidaan saada tietoa myös niiden kehityksestä
Russ, Jane Hannah. "Use of organ-culture, irradiation and adoptive-transfer to investigate the role of the Xenopus thymus in T lymphocyte development." Thesis, Durham University, 1986. http://etheses.dur.ac.uk/6885/.
Full textKonduri, Suchitra. "The Influence of normal physiological forces on porcine aortic heart valves in a sterile ex-vivo pulsatile organ culture system." Thesis, Available online, Georgia Institute of Technology, 2005, 2005. http://etd.gatech.edu/theses/available/etd-03042005-135623/unrestricted/konduri%5Fsuchitra%5F200505%5Fmast.pdf.
Full textDr. Athanassios Sambanis, Committee Member ; Dr. Timothy M. Wick, Committee Member ; Dr. Ajit P.Yoganathan, Committee Chair. Includes bibliographical references.
Konda, Bikash [Verfasser], and Jutta [Akademischer Betreuer] Engel. "Response of mammalian eye lenses to space radiation qualities in vitro and in organ culture / Bikash Konda ; Betreuer: Jutta Engel." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2019. http://d-nb.info/1205735216/34.
Full textAbdin, Alaa Din [Verfasser]. "Impact of dextran in organ culture media for preservation of DMEK (Descemet Membrane Endothelial Keratoplasty) precut tissue / Alaa Din Abdin." Saarbrücken : Saarländische Universitäts- und Landesbibliothek, 2020. http://d-nb.info/1216104832/34.
Full textHreinsson, Julius. "Preservation of fertility through cryopreservation and in vitro maturation of human ovarian follicles and oocytes /." Stockholm, 2003. http://diss.kib.ki.se/2003/91-7349-698-7.
Full textJunttila, S. (Sanna). "Studies of kidney induction in vitro using gene expression profiling and novel tissue manipulation technique." Doctoral thesis, Oulun yliopisto, 2014. http://urn.fi/urn:isbn:9789526206608.
Full textTiivistelmä Nisäkkäiden munuainen on toiminut vuosikymmeniä mallielimenä tutkittaessa kehitysbiologisia tapahtumasarjoja, kuten epitelisaatiota, haaroittumismorfologiaa sekä solujen erilaistumista. Munuaisaihioita voidaan viljellä laboratorio-olosuhteissa, jolloin kehityksen aikaisia muutoksia päästään seuraamaan lähes reaaliaikaisesti. Perinteisten kudosviljelytekniikoiden tarjoamat mahdollisuudet solujen molekulaariseen muokkaukseen ovat kuitenkin varsin rajalliset. Tässä väitöskirjassa esitettävät tulokset pyrkivät osaltaan vähentämään näitä rajoitteita. Väitöskirjan kokeellisessa osassa tarkastellaan lähemmin klassista munuaiskudosviljelyä sekä esitetään siihen tehtyjä optimointeja, joiden avulla kudosviljelyä pyritään hyödyntämään geenien toiminnan tutkimuksessa. Aluksi perinteisellä tavalla reikäisen kalvon läpi indusoitu nefroni karakterisoitiin tarkasti hyödyntäen useita erilaistumista osoittavia merkkimolekyylejä. Lisäksi samalla tekniikalla tuotettujen munuaiskudosviljelmien geeniekspressiota tutkittiin mikrosiruanalyysillä. Klassisia kudosviljelytekniikoita muokattiin soveltuvammaksi moderneille geneettisille työkaluille. Munuaiskudos hajotettiin ensin solususpensioksi, jonka jälkeen solut muodostivat uudelleen kolmiulotteisen, kudosmaisen rakenteen. Hyödyntämällä suojaavia kasvutekijöitä, hajotus kyettiin tekemään jo ennen nefronien muodostumisen alkua. Näin saavutettin 24 tunnin aikaikkuna indusoimattoman kudoksen geneettiselle muokkaukselle. Väitöskirjassa esitelläänkin demonsrtaatiot solujen lisäämisestä ja poistamisesta sekä virusvälitteisestä geenin aktivoinnista ja hiljennyksestä hyödyntäen uutta kudosmanipulaatio ja –vilejelytekniikkaa. Nefronin kehityksen aikaisen geeniekspression kartoitus sekä tässä tutkimuksessa kehitetyt uudet kudosmanipulaatio ja -viljelytekniikat tarjoavat yhdessä työkaluja molekyylitason yksityiskohtaiseen tutkimiseen
Korecki, Casey. "Effects of Compression Loading, Injury, and Age on Intervertebral Disc Mechanics, Biology and Metabolism Using Large Animal Organ and Cell Culture Systems." ScholarWorks @ UVM, 2008. http://scholarworks.uvm.edu/graddis/126.
Full textDas, Pritam. "Membrane micro-structurée utilisable comme support au développement de cellule humaine : développement, caractérisation et interaction cellule-matrice." Thesis, Toulouse 3, 2018. http://www.theses.fr/2018TOU30282/document.
Full textOver the last decades, three-dimensional (3D) scaffolds are unfolding many promising applications in tissue engineering and regenerative medicine field by providing suitable microenvironment for the incorporation of cells or growth factors to regenerate damaged tissues or organs. The three-dimensional polymeric porous scaffolds with higher porosities having homogeneous interconnected pore network are highly useful for tissue engineering. In this context, a poly (ε- caprolactone) PCL/chitosan CHT blend membrane with a double porous morphology was developed by modified liquid induced phase inversion technique. The membrane shows: (i) surface macrovoids (big pores) which could be easily accessible for cells invasion and viability; (ii) interconnected microporous (small pores) network to transfer essential nutrients, oxygen, growth factors between the macrovoids and throughout the scaffolds. The physico-chemical properties (pore size, surface chemistry and biodegradability) of the materials have been characterized. This study shows how it is possible to tune the membrane properties by changing the PCL/CHT ratio. Human mesenchymal stem cell (hMSCs) culture was performed on the membranes and the cell viability and proliferation was investigated by MTT assay and oxygen uptake rate experiments. The experiments demonstrate that the membranes are biocompatible and can be colonized by the cells at micron scale. Confocal microscopy images show that the cells are able to adhere and penetrate inside the macrovoids of the membranes. Both cell proliferation and oxygen uptake increase with time especially on membranes with lower chitosan concentration. The presence of chitosan in the blend produces an increase of porosity that affect the entrapment of the cells inside the porous bulk of the membranes. Successful cellular proliferation of hMSCs could be useful to enhance longevity of other primary cells by production of corresponding growth factors. To test the dynamic behavior of cells on the membranes, an organ-on-chip (OOC) device has been developed with human umbilical endothelial cells (HUVECs) seeded on the membrane. The hydraulic resistance of the cellular barrier on the membrane has been quantified for real time trans-endothelial pressure (TEP) 20 cmH2O at 37 degree C and with living cells after 1 day and 3 day of post seeding. Results suggests this kind of polymeric scaffolds can be useful in future as an in vivo patch to repair disrupted vessels
Khidhir, Karzan Ghafur. "Regulation of hair growth : prostaglandins and prostamides : studies confirming the growth stimulating effects of prostanoids and prostamides on human hair follicles in organ culture and locating their receptors using lipidomics, molecular biological and immunohistological approaches." Thesis, University of Bradford, 2010. http://hdl.handle.net/10454/5372.
Full textCastro, Mirna. "Pharmacokinetic studies on protoporphyrin IX induced by 5-aminolevulinic acid and its esters in a three-dimensional lung tumor mini-organ culture model." Diss., lmu, 2005. http://nbn-resolving.de/urn:nbn:de:bvb:19-33239.
Full textBecker, Daniel. "At the intersection of the clinic and the laboratory : the invention, dissemination, and application of organ replacement therapy in late-Victorian medical culture." Thesis, Durham University, 2014. http://etheses.dur.ac.uk/10627/.
Full text