Relevant bibliographies by topics / Operoni

Academic literature on the topic 'Operoni'

Author: Grafiati
Published: 10 March 2023

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Journal articles on the topic "Operoni"

1

Nguyen, Huy N., Ashish Jain, Oliver Eulenstein, and Iddo Friedberg. "Tracing the ancestry of operons in bacteria." Bioinformatics 35, no. 17 (January 24, 2019): 2998–3004. http://dx.doi.org/10.1093/bioinformatics/btz053.

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Abstract Motivation Complexity is a fundamental attribute of life. Complex systems are made of parts that together perform functions that a single component, or subsets of components, cannot. Examples of complex molecular systems include protein structures such as the F1Fo-ATPase, the ribosome, or the flagellar motor: each one of these structures requires most or all of its components to function properly. Given the ubiquity of complex systems in the biosphere, understanding the evolution of complexity is central to biology. At the molecular level, operons are classic examples of a complex system. An operon’s genes are co-transcribed under the control of a single promoter to a polycistronic mRNA molecule, and the operon’s gene products often form molecular complexes or metabolic pathways. With the large number of complete bacterial genomes available, we now have the opportunity to explore the evolution of these complex entities, by identifying possible intermediate states of operons. Results In this work, we developed a maximum parsimony algorithm to reconstruct ancestral operon states, and show a simple vertical evolution model of how operons may evolve from the individual component genes. We describe several ancestral states that are plausible functional intermediate forms leading to the full operon. We also offer Reconstruction of Ancestral Gene blocks Using Events or ROAGUE as a software tool for those interested in exploring gene block and operon evolution. Availability and implementation The software accompanying this paper is available under GPLv3 license on: https://github.com/nguyenngochuy91/Ancestral-Blocks-Reconstruction. Supplementary information Supplementary data are available at Bioinformatics online.
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2

Brandis, Gerrit, Sha Cao, and Diarmaid Hughes. "Operon Concatenation Is an Ancient Feature That Restricts the Potential to Rearrange Bacterial Chromosomes." Molecular Biology and Evolution 36, no. 9 (May 27, 2019): 1990–2000. http://dx.doi.org/10.1093/molbev/msz129.

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Abstract The last common ancestor of the Gammaproteobacteria carried an important 40-kb chromosome section encoding 51 proteins of the transcriptional and translational machinery. These genes were organized into eight contiguous operons (rrnB-tufB-secE-rpoBC-str-S10-spc-alpha). Over 2 Gy of evolution, in different lineages, some of the operons became separated by multigene insertions. Surprisingly, in many Enterobacteriaceae, much of the ancient organization is conserved, indicating a strong selective force on the operons to remain colinear. Here, we show for one operon pair, tufB-secE in Salmonella, that an interruption of contiguity significantly reduces growth rate. Our data show that the tufB-secE operons are concatenated by an interoperon terminator–promoter overlap that plays a significant role regulating gene expression. Interrupting operon contiguity interferes with this regulation, reducing cellular fitness. Six operons of the ancestral chromosome section remain contiguous in Salmonella (tufB-secE-rpoBC and S10-spc-alpha) and, strikingly, each of these operon pairs is also connected by an interoperon terminator–promoter overlap. Accordingly, we propose that operon concatenation is an ancient feature that restricts the potential to rearrange bacterial chromosomes and can select for the maintenance of a colinear operon organization over billions of years.
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Ming, Li, and Anna Boiko. "Recipes of the Russian opera tradition in the Chinese opera art of the 20th century." OOO "Zhurnal "Voprosy Istorii" 2020, no. 11-1 (November 1, 2020): 170–78. http://dx.doi.org/10.31166/voprosyistorii202011statyi07.

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The publication is devoted to the consideration of the peculiarities of the reception of Russian opera tradition in the Chinese opera of 20th century. The significant role of the historical and the cultural events that affected the spread Russian opera in China is analyzed. Particular attention is paid to the study of the activities of representatives the Russian emigration in the field of opera art. It was emphasized that the influence of Russian and foreign opera contributed to the formation of the genre the Chinese opera of the European type.
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Acinas, Silvia G., Luisa A. Marcelino, Vanja Klepac-Ceraj, and Martin F. Polz. "Divergence and Redundancy of 16S rRNA Sequences in Genomes with Multiple rrn Operons." Journal of Bacteriology 186, no. 9 (May 1, 2004): 2629–35. http://dx.doi.org/10.1128/jb.186.9.2629-2635.2004.

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ABSTRACT The level of sequence heterogeneity among rrn operons within genomes determines the accuracy of diversity estimation by 16S rRNA-based methods. Furthermore, the occurrence of widespread horizontal gene transfer (HGT) between distantly related rrn operons casts doubt on reconstructions of phylogenetic relationships. For this study, patterns of distribution of rrn copy numbers, interoperonic divergence, and redundancy of 16S rRNA sequences were evaluated. Bacterial genomes display up to 15 operons and operon numbers up to 7 are commonly found, but ∼40% of the organisms analyzed have either one or two operons. Among the Archaea, a single operon appears to dominate and the highest number of operons is five. About 40% of sequences among 380 operons in 76 bacterial genomes with multiple operons were identical to at least one other 16S rRNA sequence in the same genome, and in 38% of the genomes all 16S rRNAs were invariant. For Archaea, the number of identical operons was only 25%, but only five genomes with 21 operons are currently available. These considerations suggest an upper bound of roughly threefold overestimation of bacterial diversity resulting from cloning and sequencing of 16S rRNA genes from the environment; however, the inclusion of genomes with a single rrn operon may lower this correction factor to ∼2.5. Divergence among operons appears to be small overall for both Bacteria and Archaea, with the vast majority of 16S rRNA sequences showing <1% nucleotide differences. Only five genomes with operons with a higher level of nucleotide divergence were detected, and Thermoanaerobacter tengcongensis exhibited the highest level of divergence (11.6%) noted to date. Overall, four of the five extreme cases of operon differences occurred among thermophilic bacteria, suggesting a much higher incidence of HGT in these bacteria than in other groups.
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Yap, Wai Ho, Zhenshui Zhang, and Yue Wang. "Distinct Types of rRNA Operons Exist in the Genome of the Actinomycete Thermomonospora chromogena and Evidence for Horizontal Transfer of an Entire rRNA Operon." Journal of Bacteriology 181, no. 17 (September 1, 1999): 5201–9. http://dx.doi.org/10.1128/jb.181.17.5201-5209.1999.

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ABSTRACT We describe here the presence of two distinct types of rRNA operons in the genome of a thermophilic actinomycete Thermomonospora chromogena. The genome of T. chromogena contains six rRNA operons (rrn), of which four complete and two incomplete ones were cloned and sequenced. Comparative analysis revealed that the operon rrnB exhibits high levels of sequence variations to the other five nearly identical ones throughout the entire length of the operon. The coding sequences for the 16S and 23S rRNA genes differ by approximately 6 and 10%, respectively, between the two types of operons. Normal functionality ofrrnB is concluded on the basis of the nonrandom distribution of nucleotide substitutions, the presence of compensating nucleotide covariations, the preservation of secondary and tertiary rRNA structures, and the detection of correctly processed rRNAs in the cell. Comparative sequence analysis also revealed a close evolutionary relationship between rrnB operon of T. chromogena and rrnA operon of another thermophilic actinomycete Thermobispora bispora. We propose thatT. chromogena acquired rrnB operon fromT. bispora or a related organism via horizontal gene transfer.
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Dennis, Patrick P., Sonia Ziesche, and Shanthini Mylvaganam. "Transcription Analysis of Two Disparate rRNA Operons in the Halophilic Archaeon Haloarcula marismortui." Journal of Bacteriology 180, no. 18 (September 15, 1998): 4804–13. http://dx.doi.org/10.1128/jb.180.18.4804-4813.1998.

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ABSTRACT The genome of the halophilic archaeon Haloarcula marismortui contains two rRNA operons designated rrnAand rrnB. Genomic clones of the two operons and their flanking regions have been sequenced, and primary transcripts and processing intermediates derived from each operon have been characterized. The 16S, 23S, and 5S genes from the two operons were found to differ at 74 of 1,472 positions, 39 of 2,922 positions, and 2 of 122 positions, respectively. This degree of sequence divergence for multicopy (paralogous) rRNA genes was 10- to 50-fold or more higher than anticipated. The two operons exhibit other profound differences that include (i) the presence in rrnA and the absence inrrnB of tRNAAla and tRNACys genes in the intergenic and distal regions, respectively, (ii) divergent 5′ flanking sequences, and (iii) distinct pathways for processing and maturation of 16S rRNA. Processing and maturation of 16S and 23S rRNA from rrnA operon transcripts and of 23S rRNA fromrrnB operon transcripts follow the canonical halophilic pathway, whereas maturation of 16S rRNA from rrnB operon transcripts follows an unusual and different pathway that is apparently devoid of any 5′ processing intermediate.
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Reksoprawiro, Ricky, Gabriella Scarlett, Alexander Joseph Ibnu Wibowo, and Novi Amelia. "Consumer Culture Theory: Hubungan Timbal Balik antar Social Operant Resources dan Operand Resources dalam Studi Empiris McDonald’s Indonesia." Kajian Branding Indonesia 2, no. 1 (January 13, 2020): 132–61. http://dx.doi.org/10.21632/kbi.2.1.132-161.

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Penelitian ini bertujuan untuk menguji secara empiris secara garis besar untuk mengetahui hubungan antara konsep social operant resources terhadap operand resources yang dilandasi oleh consumer culture theory. Secara spesifik, studi ini ingin menguji sebagai berikut: (i) pengaruh social operant resources terhadap economic operand resources; (ii) pengaruh social operant resources terhadap material operand resources; (iii) pengaruh economic operand resources terhadap social operant resources; dan (iv) pengaruh material operand resources terhadap social operant resources; (v) hubungan timbal balik antara social operant resources dan economic operand resources; (vi) hubungan timbal balik antara social operant resources dan material operand resources. Permasalahan dalam studi ini ditelaah dengan berbasis pada konsep consumer culture theory (CCT). Pengumpulan data primer dilakukan menggunakan kuesioner melalui survei online. Seluruh indikator diukur menggunakan skala Likert tujuh poin. Penelitian ini berhasil memperoleh data responden sebanyak 358 orang konsumen restoran McDonald’s. Teknik analisis faktor eksploratori exploratory factor analysis (EFA) diterapkan untuk melakukan uji validitas konstruk. Selanjutnya, keenam hipotesis diuji menggunakan teknik structural equation modeling (SEM). Hasil studi ini berhasil membuktikan bahwa keenam hipotesis yang diajukan tidak ditolak. Secara ringkas, social operant resources memengaruhi economic operand resources, social operant resources memengaruhi material operand resources, economic operand resources memengaruhi social operant resources, material operand resources memengaruhi social operant resources, ada hubungan timbal balik antara social operant resources dan economic material resources, dan ada hubungan timbal balik antara social operant resources dan material operand resources.
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Menendez, M. C., M. J. Garcia, M. C. Navarro, J. A. Gonzalez-y-Merchand, S. Rivera-Gutierrez, L. Garcia-Sanchez, and R. A. Cox. "Characterization of an rRNA Operon (rrnB) of Mycobacterium fortuitum and Other Mycobacterial Species: Implications for the Classification of Mycobacteria." Journal of Bacteriology 184, no. 4 (February 15, 2002): 1078–88. http://dx.doi.org/10.1128/jb.184.4.1078-1088.2002.

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ABSTRACT Mycobacteria are thought to have either one or two rRNA operons per genome. All mycobacteria investigated to date have an operon, designated rrnA, located downstream from the murA gene. We report that Mycobacteriun fortuitum has a second rrn operon, designated rrnB, which is located downstream from the tyrS gene; tyrS is very close to the 3" end of a gene (3-mag) coding for 3-methylpurine-DNA-glycosylase. The second rrn operon of Mycobacterium smegmatis was shown to have a similar organization, namely, 5" 3-mag-tyrS-rrnB 3". The rrnB operon of M. fortuitum was found to have a single dedicated promoter. During exponential growth in a rich medium, the rrnB and rrnA operons were the major and minor contributors, respectively, to pre-rRNA synthesis. Genomic DNA was isolated from eight other fast-growing mycobacterial species. Samples were investigated by Southern blot analysis using probes for murA, tyrS, and 16S rRNA sequences. The results revealed that both rrnA and rrnB operons were present in each species. The results form the basis for a proposed new scheme for the classification of mycobacteria. The approach, which is phylogenetic in concept, is based on particular properties of the rrn operons of a cell, namely, the number per genome and a feature of 16S rRNA gene sequences.
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Asai, Tsuneaki, Ciarán Condon, Justina Voulgaris, Dmitry Zaporojets, Binghua Shen, Michaal Al-Omar, Craig Squires, and Catherine L. Squires. "Construction and Initial Characterization of Escherichia coli Strains with Few or No Intact Chromosomal rRNA Operons." Journal of Bacteriology 181, no. 12 (June 15, 1999): 3803–9. http://dx.doi.org/10.1128/jb.181.12.3803-3809.1999.

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ABSTRACT The Escherichia coli genome carries seven rRNA (rrn) operons, each containing three rRNA genes. The presence of multiple operons has been an obstacle to many studies of rRNA because the effect of mutations in one operon is diluted by the six remaining wild-type copies. To create a tool useful for manipulating rRNA, we sequentially inactivated from one to all seven of these operons with deletions spanning the 16S and 23S rRNA genes. In the final strain, carrying no intact rRNA operon on the chromosome, rRNA molecules were expressed from a multicopy plasmid containing a single rRNA operon (prrn). Characterization of these rrndeletion strains revealed that deletion of two operons was required to observe a reduction in the growth rate and rRNA/protein ratio. When the number of deletions was extended from three to six, the decrease in the growth rate was slightly more than the decrease in the rRNA/protein ratio, suggesting that ribosome efficiency was reduced. This reduction was most pronounced in the Δ7 prrn strain, in which the growth rate, unlike the rRNA/protein ratio, was not completely restored to wild-type levels by a cloned rRNA operon. The decreases in growth rate and rRNA/protein ratio were surprisingly moderate in the rrndeletion strains; the presence of even a single operon on the chromosome was able to produce as much as 56% of wild-type levels of rRNA. We discuss possible applications of these strains in rRNA studies.
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Lakhova, Tatiana N., Fedor V. Kazantsev, Aleksey M. Mukhin, Nikolay A. Kolchanov, Yury G. Matushkin, and Sergey A. Lashin. "Algorithm for the Reconstruction of Mathematical Frame Models of Bacterial Transcription Regulation." Mathematics 10, no. 23 (November 28, 2022): 4480. http://dx.doi.org/10.3390/math10234480.

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Transcription regulation plays an important role in bacterial activity. The operon concept coined by François Jacob and Jacques Monod has had a considerable effect on investigations into gene expression regulation, including modeling. However, most such studies have considered the regulation models devised manually for one or several operons. For that reason, the objective of the present study was automated genome model reconstruction for different bacteria. The suggested algorithm accounted for all possible interactions of transcription factors and their binding sites in an operon’s promoter region. Transcription factor enumeration was performed using the deep-first search technique. The obtained models are of interest for those involved in the research of transcription factor regulatory effects on bacterial gene expression in microbiology and biotechnology.
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Dissertations / Theses on the topic "Operoni"

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Fortino, Vittorio. "Sequence analysis in bioinformatics: methodological and practical aspects." Doctoral thesis, Universita degli studi di Salerno, 2013. http://hdl.handle.net/10556/985.

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2011 - 2012
My PhD research activities has focused on the development of new computational methods for biological sequence analyses. To overcome an intrinsic problem to protein sequence analysis, whose aim was to infer homologies in large biological protein databases with short queries, I developed a statistical framework BLAST-based to detect distant homologies conserved in transmembrane domains of different bacterial membrane proteins. Using this framework, transmembrane protein domains of all Salmonella spp. have been screened and more than five thousands of significant homologies have been identified. My results show that the proposed framework detects distant homologies that, because of their conservation in distinct bacterial membrane proteins, could represent ancient signatures about the existence of primeval genetic elements (or mini-genes) coding for short polypeptides that formed, through a primitive assembly process, more complex genes. Further, my statistical framework lays the foundation for new bioinformatics tools to detect homologies domain-oriented, or in other words, the ability to find statistically significant homologies in specific target-domains. The second problem that I faced deals with the analysis of transcripts obtained with RNA-Seq data. I developed a novel computational method that combines transcript borders, obtained from mapped RNA-Seq reads, with sequence features based operon predictions to accurately infer operons in prokaryotic genomes. Since the transcriptome of an organism is dynamic and condition dependent, the RNA-Seq mapped reads are used to determine a set of confirmed or predicted operons and from it specific transcriptomic features are extracted and combined with standard genomic features to train and validate three operon classification models (Random Forests - RFs, Neural Networks – NNs, and Support Vector Machines - SVMs). These classifiers have been exploited to refine the operon map annotated by DOOR, one of the most used database of prokaryotic operons. This method proved that the integration of genomic and transcriptomic features improve the accuracy of operon predictions, and that it is possible to predict the existence of potential new operons. An inherent limitation of using RNA-Seq to improve operon structure predictions is that it can be not applied to genes not expressed under the condition studied. I evaluated my approach on different RNA-Seq based transcriptome profiles of Histophilus somni and Porphyromonas gingivalis. These transcriptome profiles were obtained using the standard RNA-Seq or the strand-specific RNA-Seq method. My experimental results demonstrate that the three classifiers achieved accurate operon maps including reliable predictions of new operons. [edited by author]
XI n.s.
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Dimitrovska, Valentina Metodi <1978&gt. "Study of two novel streptococcus pneumoniae operons: pilus islet 2 and the lantibiotic operon." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2010. http://amsdottorato.unibo.it/2615/1/Dimitrovska_Valentina_PhD_thesis.pdf.

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Analysis of publicly available genomes of Streptococcus pneumoniae has led to the identification of a new genomic element resembling gram-positive pilus islets (PIs). Here, we demonstrate that this genomic region, herein referred to as PI-2 (containing the genes pitA, sipA, pitB, srtG1, and srtG2) codes for a novel functional pilus in pneumococcus. Therefore, there are two pilus islets identified so far in this pathogen (PI-1 and PI-2). Polymerization of the PI-2 pilus requires the backbone protein PitB as well as the sortase SrtG1 and the signal peptidase-like protein SipA. PI-2 is associated with serotypes 1, 2, 7F, 19A, and 19F, considered to be emerging in both industrialized and developing countries. Interestingly, strains belonging to clonal complex 271 (CC271) contain both PI-1 and PI-2, as revealed by genome analyses. In these strains both pili are surface exposed and independently assembled. Furthermore, in vitro experiments provide evidence that the pilus encoded by PI-2 of S. pneumoniae is involved in adherence. Thus, pneumococci encode at least two types of pili that may play a role in the initial host cell contact to the respiratory tract. In addition, the pilus proteins are potential antigens for inclusion in a new generation of pneumococcal vaccines. Adherence by pili could represent important factor in bacterial community formation, since it has been demonstrated that bacterial community formation plays an important role in pneumococcal otitis media. In vitro quantification of bacterial community formation by S. pneumoniae was performed in order to investigate the possible role of pneumococcal pili to form communities. By using different growth media we were not able to see clear association between pili and community formation. But our findings revealed that strains belonging to MLST clonal complex CC15 efficiently form bacterial communities in vitro in a glucose dependent manner. We compared the genome of forty-four pneumococcal isolates discovering four open reading frames specifically associated with CC15. These four genes are annotated as members of an operon responsible for the biosynthesis of a putative lanctibiotic peptide, described to be involved in bacterial community formation. Our experiments show that the lanctibiotic operon deletion affects glucose mediated community formation in CC 15 strain INV200. Moreover, since glucose consumption during bacterial growth produce an acidic environment, we tested bacterial community formation at different pH and we showed that the lanctibiotic operon deletion affected pH mediated community formation in CC 15 strain INV200. In conclusion, these data demonstrate that the putative lanctibiotic operon is associated with pneumococcal CC 15 strains in vitro bacterial community formation.
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Dimitrovska, Valentina Metodi <1978&gt. "Study of two novel streptococcus pneumoniae operons: pilus islet 2 and the lantibiotic operon." Doctoral thesis, Alma Mater Studiorum - Università di Bologna, 2010. http://amsdottorato.unibo.it/2615/.

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Analysis of publicly available genomes of Streptococcus pneumoniae has led to the identification of a new genomic element resembling gram-positive pilus islets (PIs). Here, we demonstrate that this genomic region, herein referred to as PI-2 (containing the genes pitA, sipA, pitB, srtG1, and srtG2) codes for a novel functional pilus in pneumococcus. Therefore, there are two pilus islets identified so far in this pathogen (PI-1 and PI-2). Polymerization of the PI-2 pilus requires the backbone protein PitB as well as the sortase SrtG1 and the signal peptidase-like protein SipA. PI-2 is associated with serotypes 1, 2, 7F, 19A, and 19F, considered to be emerging in both industrialized and developing countries. Interestingly, strains belonging to clonal complex 271 (CC271) contain both PI-1 and PI-2, as revealed by genome analyses. In these strains both pili are surface exposed and independently assembled. Furthermore, in vitro experiments provide evidence that the pilus encoded by PI-2 of S. pneumoniae is involved in adherence. Thus, pneumococci encode at least two types of pili that may play a role in the initial host cell contact to the respiratory tract. In addition, the pilus proteins are potential antigens for inclusion in a new generation of pneumococcal vaccines. Adherence by pili could represent important factor in bacterial community formation, since it has been demonstrated that bacterial community formation plays an important role in pneumococcal otitis media. In vitro quantification of bacterial community formation by S. pneumoniae was performed in order to investigate the possible role of pneumococcal pili to form communities. By using different growth media we were not able to see clear association between pili and community formation. But our findings revealed that strains belonging to MLST clonal complex CC15 efficiently form bacterial communities in vitro in a glucose dependent manner. We compared the genome of forty-four pneumococcal isolates discovering four open reading frames specifically associated with CC15. These four genes are annotated as members of an operon responsible for the biosynthesis of a putative lanctibiotic peptide, described to be involved in bacterial community formation. Our experiments show that the lanctibiotic operon deletion affects glucose mediated community formation in CC 15 strain INV200. Moreover, since glucose consumption during bacterial growth produce an acidic environment, we tested bacterial community formation at different pH and we showed that the lanctibiotic operon deletion affected pH mediated community formation in CC 15 strain INV200. In conclusion, these data demonstrate that the putative lanctibiotic operon is associated with pneumococcal CC 15 strains in vitro bacterial community formation.
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Carmichael, C. S. J. "Decomposition of the lactose operon." Thesis, University of Edinburgh, 1992. http://hdl.handle.net/1842/13315.

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The immediate aims of the project, as set out in the introduction, were 1) to separate the lacZ and lacY genes of the lactose operon such that they could be controlled/induced independently 2) to maintain the expression construct in the E.coli chromosome. The lacY gene was subcloned into plasmid PBN372 downstream of the S.marsescens trp promoter. The flanking E.coli trp genes were exploited to integrate the construct into the E.coli chromosome at the trpB locus via homologous recombination. Homologous recombinants should be trp{-}. Two approaches were employed to achieve integration: 1) transformation of a recD strain (which was also a lacY deletion) 2) transduction with phage lambda. The first method was unsuccessful, only spontaneous trp- mutations were isolated. The second method yielded several integrants, one of which was used in subsequent growth experiments. Since the constructed strain was rendered trp-, the internal tryptophan concentration could be influenced by the concentration of tryptophan in the medium and the level of induction could be set by the addition of differing amounts of the antirepressor, IAA. The growth rate of the constructed strain in minimal lactose media was comparable with that of wild type E.coli. The permease activity of the constructed strain was seen to vary when assayed in the presence of varying amounts of IAA. The expression of permease was also demonstrated to be independent of galactosidase activity. The constructed strain therefore met all the initial requirements for the experimental system set out in this thesis. Difficulties were encountered during the analysis of permease induction in batch culture. This was due to the competition between antirepressor (IAA) and corepressor (tryptophan). These difficulties would have been minimal had the analysis been carried out in chemostat experiments. It would then have been possible to maintain a constant, low level of tryptophan throughout the experiment. In batch culture, the tryptophan level is constantly changing, initially being relatively high and becoming depleted as the experiment progresses.
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Upadhyay, Manisha. "The flp operons of Lactococcus lactis." Thesis, University of Sheffield, 2002. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.274956.

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Flick, Jeremy Joseph. "Modus Operandi." College Park, Md.: University of Maryland, 2009. http://hdl.handle.net/1903/9372.

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Thesis (M.F.A.) -- University of Maryland, College Park, 2009.
Thesis research directed by: Dept. of Art. Title from t.p. of PDF. Includes bibliographical references. Published by UMI Dissertation Services, Ann Arbor, Mich. Also available in paper.
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Patterson, Kathryn Grace. "Gene regulation in the lac operon." Thesis, Montana State University, 2009. http://etd.lib.montana.edu/etd/2009/patterson/PattersonK0809.pdf.

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The lac operon, a jointly controlled series of genes in the bacteria E. coli, has been studied extensively since the 1940's. The lac operon genes are transcribed and then translated into proteins necessary for transport and digestion of lactose. The operon is activated in the presence of lactose after glucose, the preferred carbon source, has been expended. In this thesis, we introduce a biophysical model using the Shea-Ackers framework for modeling promoter dynamics. The model spans two scales: the inputs are biophysical parameters of molecular interactions and the result is a level of gene expression - a macroscopic behavior of the cell. We include all experimentally suggested control mechanisms into the model, even though the experimental evidence is stronger for some of these mechanisms than others. We compare our model to experimental data and explore the individual contribution of the proposed mechanisms by removing them one by one and testing the reduced model's fit to the data. Finally, we find a minimal model which faithfully represents the available data, yet includes only the minimal number of control mechanisms.
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Uhlmann, Mareike. "Mutagenese des nuo-Operons von Escherichia coli." [S.l.] : [s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=975459414.

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Curtis, Mark Douglas. "An investigation of the E9 colicin 'operon'." Thesis, University of East Anglia, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.304985.

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Pandey, Sundar. "Novel Role of Pseudomonas Aeruginosa LptD Operon." FIU Digital Commons, 2018. https://digitalcommons.fiu.edu/etd/3734.

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Pseudomonas aeruginosais an opportunistic pathogen that infects cystic fibrosis (CF) patients contributing to their high morbidity and mortality. P. aeruginosaundergoes a phenotypic conversion in the CF lung, from nonmucoid to mucoid, by constitutively producing a polysaccharide called alginate. These mucoid strains often revert to nonmucoid in vitrodue to second-site suppressor mutations. We hypothesized that mapping these mutations would lead to the identification of novel genes involved in alginate production. In a previous study, a mucoid strain, PDO300 (PAOmucA22), was used to isolate suppressors of alginate phenotype (sap). One of the uncharacterized nonmucoid revertants, sap27, is the subject of this study. The mucoid phenotype in sap27was restored by pMO012217 from a minimal tiling path cosmid library. The cosmid pMO012217 harbors 18 P. aeruginosaopen reading frames (ORF). The cosmid was mutagenized with a transposon to map the contributing gene. It was mapped tolptD(PA0595) encoding lipopolysaccharide transport protein. E. coliLptD transports lipopolysaccharide to the outer leaflet of the outer membrane. The Alg+phenotype was restored upon complementation with P. aeruginosa lptDalone, suggesting that sap27likely harbor a chromosomal mutation inlptD. Sequencing analysis of sap27showed the presence of a mutation not in lptDbut in algO, which encodes a periplasmic protease protein. This suggests LptD is able to bypass analgO mutation by positively regulating alginate production. The lptD is a part of a three-gene operon lptD-surA-pdxA. SurA is an essential protein for survival in starvation and a major chaperone protein for all outer membrane proteins and PdxA is a NAD-dependent dehydrogenase and is involved in the vitamin B6biosynthetic pathway. Pyridoxal 5’-phosphate (PLP) is the active form of vitamin B6.P. aeruginosagrown in a media supplemented with PLP increased production of pyocyanin, a virulence factor. The PLP and aromatic amino acids are synthesized from a common precursor chorismic acid. We demonstrated an increase in pyocyanin production when the bacteria were cultured supplemented by the aromatic amino acids phenylalanine. We concluded that the lptDoperon plays a role in the P. aeruginosavirulence by regulating alginate and pyocyanin production.
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Books on the topic "Operoni"

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Ĭoncheva, Gali︠a︡. Operni pŭteki. Sofii︠a︡: Izd-vo "Sibi", 2003.

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Hrvatski operni pjevači. Zagreb: Nakladni zavod Matice hrvatske, 1996.

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Prabowo, J. S. Operasi lawan insurjensi: Bukan hanya operasi militer. Jakarta: PPSN, 2013.

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Przewodnik operowy. Kraków: Polskie Wydawnictwo Muzyczne, 2014.

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Haris, Zahakir. Operasi Ganesa. Bandung: Alumni, 1988.

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Afghani, C. N. al. Operasi Kenari. Kuala Lumpur: Penerbitan Pemuda, 1990.

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Dewan Bahasa dan Pustaka Brunei., ed. Penyelidikan operasi. Bandar Seri Begawan, Brunei Darussalam: Dewan Bahasa dan Pustaka Brunei, Kementerian Kebudayaan, Belia dan Sukan, 1994.

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Djati, Kerami, and Pusat Pembinaan dan Pengembangan Bahasa., eds. Riset operasi. Jakarta: Pusat Pembinaan dan Pengembangan Bahasa, Departemen Pendidikan dan Kebudayaan, 1994.

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Stefano, Musso. Operai. Torino: Rosenberg & Sellier, 2006.

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Davis, Hank, and Harry M. B. Hurwitz. Operant-Pavlovian Interactions. London: Routledge, 2021. http://dx.doi.org/10.4324/9781003150404.

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Book chapters on the topic "Operoni"

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Briones, Carlos. "Operon." In Encyclopedia of Astrobiology, 1177. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-11274-4_1115.

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Briones, Carlos. "Operon." In Encyclopedia of Astrobiology, 1778. Berlin, Heidelberg: Springer Berlin Heidelberg, 2015. http://dx.doi.org/10.1007/978-3-662-44185-5_1115.

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Gooch, Jan W. "Operon." In Encyclopedic Dictionary of Polymers, 912. New York, NY: Springer New York, 2011. http://dx.doi.org/10.1007/978-1-4419-6247-8_14389.

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Briones, Carlos. "Operon." In Encyclopedia of Astrobiology, 1. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-27833-4_1115-3.

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Hausmann, Rudolf. "The Operon." In To Grasp the Essence of Life, 191–96. Dordrecht: Springer Netherlands, 2002. http://dx.doi.org/10.1007/978-94-017-3540-7_15.

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Tagami, Hideaki. "Operon Theory." In Encyclopedia of Systems Biology, 1568–69. New York, NY: Springer New York, 2013. http://dx.doi.org/10.1007/978-1-4419-9863-7_1400.

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Mizuno, Tooru M., Ashwini Padhi, Naomi Fineberg, Naomi A. Fineberg, Ashwini Padhi, Michael H. Bloch, James F. Leckman, et al. "Operant." In Encyclopedia of Psychopharmacology, 926. Berlin, Heidelberg: Springer Berlin Heidelberg, 2010. http://dx.doi.org/10.1007/978-3-540-68706-1_1441.

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Weik, Martin H. "operand." In Computer Science and Communications Dictionary, 1149. Boston, MA: Springer US, 2000. http://dx.doi.org/10.1007/1-4020-0613-6_12837.

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Mackey, Michael C., Moisés Santillán, Marta Tyran-Kamińska, and Eduardo S. Zeron. "The Lactose Operon." In Lecture Notes on Mathematical Modelling in the Life Sciences, 73–85. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-45318-7_5.

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Mackey, Michael C., Moisés Santillán, Marta Tyran-Kamińska, and Eduardo S. Zeron. "The Tryptophan Operon." In Lecture Notes on Mathematical Modelling in the Life Sciences, 87–97. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-45318-7_6.

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Conference papers on the topic "Operoni"

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Liu, Derong, Zheng Zhao, Zheng Wang, Zhoufeng Ying, Ray T. Chen, and David Z. Pan. "OPERON." In DAC '18: The 55th Annual Design Automation Conference 2018. New York, NY, USA: ACM, 2018. http://dx.doi.org/10.1145/3195970.3196084.

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Burlacu, Bogdan, Gabriel Kronberger, and Michael Kommenda. "Operon C++." In GECCO '20: Genetic and Evolutionary Computation Conference. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3377929.3398099.

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Nejezchlebová, Julie, and Jana Schwarzerová. "Operon identifier: Identification of operon structures in the whole genome." In STUDENT EEICT 2022. Brno: Fakulta elektrotechniky a komunikacnich technologii VUT v Brne, 2022. http://dx.doi.org/10.13164/eeict.2022.80.

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Velazco, Sarai, Delina Kambo, Kevin Yu, Anushka Saha, Emily Beckman, Nishant Mysore, and Gert Cauwenberghs. "Modeling Gene Expression: Lac operon." In 2021 43rd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC). IEEE, 2021. http://dx.doi.org/10.1109/embc46164.2021.9630940.

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Fennedy, Katherine, and Hyowon Lee. "Modus Operandi." In Interacción 2019: XX International Conference on Human Computer Interaction. New York, NY, USA: ACM, 2019. http://dx.doi.org/10.1145/3335595.3335645.

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Chew, MonHian, Peng Wu, and Yuehui Chen. "Operon Prediction Using Broad Learning System." In 2020 International Conference on Electrical, Communication, and Computer Engineering (ICECCE). IEEE, 2020. http://dx.doi.org/10.1109/icecce49384.2020.9179272.

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Pesotskaya, K. Yu, A. L. Lagonenko, and A. N. Evtushenkov. "Investigation of molecular mechanisms involved in Erwinia amylovora biofilm formation." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.194.

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In this study we generated four insertional Erwinia amylovora mutants within bssS gene and waa, aaeXAB and hec operons. Our research included studying of the phenotypic characteristics of these mutants.
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DE HOON, M. J. L., S. IMOTO, K. KOBAYASHI, N. OGASAWARA, and S. MIYANO. "PREDICTING THE OPERON STRUCTURE OF BACILLUS SUBTILIS USING OPERON LENGTH, INTERGENE DISTANCE, AND GENE EXPRESSION INFORMATION." In Proceedings of the Pacific Symposium. WORLD SCIENTIFIC, 2003. http://dx.doi.org/10.1142/9789812704856_0027.

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Rosen, B. E., J. M. Goodwin, and J. J. Vidal. "Machine operant conditioning." In Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE, 1988. http://dx.doi.org/10.1109/iembs.1988.95349.

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Gao, Ling, Yutian Lin, Huibin Lin, Xiaoyi Jia, Jianqun Lin, and Jianqiang Lin. "Mathematical modeling of lac operon regulation dynamics." In International conference on Human Health and Medical Engineering. Southampton, UK: WIT Press, 2014. http://dx.doi.org/10.2495/hhme130611.

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Reports on the topic "Operoni"

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Tremblay, Michelle. A comparison of the effects of non-operant and operant carryover techniques for /l/. Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.3222.

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Ewing, J. Characterization of the Cobalamin and Fep Operons in Methylobium petrolphilum PM1. Office of Scientific and Technical Information (OSTI), September 2005. http://dx.doi.org/10.2172/917904.

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Corscadden, Louise, and Arpaporn Sutipatanasomboon. What Is Operant Behavior And How To Study It. Maze Engineers, December 2022. http://dx.doi.org/10.55157/me2022127.

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Operant behavior describes a type of voluntary goal-directed actions in animals based on the repercussions of previous occurrences. It develops when animals learn to specifically respond to recurring situations based on the outcome of their past experience. American psychologist B.F. Skinner was the first to use operant to describe the behaviors he observed in his landmark experiments in laboratory animals. Operant behavior and conditioning refine the nuance between conscious and unconscious behavioral responses, which influence psychology, and applied behavior analysis, and improve our understanding of addiction, substance dependence, child development, and decision-making.
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Gwynn, Mick, and Damien McDevitt. A Global Quantitative Analysis of Operon/Gene Transcription in Bacillus Anthracis During Experimental Infection. Fort Belvoir, VA: Defense Technical Information Center, July 2003. http://dx.doi.org/10.21236/ada417048.

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Carew, Thomas J. A Circuit Analysis and Computational Model of Operant Conditioning in Aplysia. Fort Belvoir, VA: Defense Technical Information Center, July 1992. http://dx.doi.org/10.21236/ada253184.

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Brembs, Björn. What is the function of FoxP in operant self-learning? [project]. ThinkLab, November 2015. http://dx.doi.org/10.15363/thinklab.11.

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Brembs, Björn. What is the function of FoxP in operant self-learning? [proposal]. ThinkLab, November 2015. http://dx.doi.org/10.15363/thinklab.a8.

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Parsons, H. M. Behavioral Determinants of Accurate Verbal Communication: An Operant Behavior-Analytic Approach. Fort Belvoir, VA: Defense Technical Information Center, September 1997. http://dx.doi.org/10.21236/ada338736.

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Carew, Thomas J. A Circuit Analysis and Computational Model of Operant Conditioning in Aplysia. Fort Belvoir, VA: Defense Technical Information Center, June 1990. http://dx.doi.org/10.21236/ada224381.

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Myers, Todd M., John C. LaMont, David W. Kahler, and Lucille A. Lumley. Effects of Repeated Sublethal VX Exposure on Operant Time Estimation in Rats. Fort Belvoir, VA: Defense Technical Information Center, August 2007. http://dx.doi.org/10.21236/ada501468.

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