Relevant bibliographies by topics / Oocyte differentiation

Academic literature on the topic 'Oocyte differentiation'

Author: Grafiati
Published: 9 March 2023
Last updated: 10 March 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Contents

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Oocyte differentiation.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Oocyte differentiation"

1

Budna, Joanna, Artur Bryja, Piotr Celichowski, Rotem Kahan, Wiesława Kranc, Sylwia Ciesiółka, Marta Rybska, et al. "Genes of cellular components of morphogenesis in porcine oocytes before and after IVM." Reproduction 154, no. 4 (October 2017): 535–45. http://dx.doi.org/10.1530/rep-17-0367.

Full text
Abstract:
Proper oocyte maturation in mammals produces an oocyte capable of monospermic fertilization and embryo preimplantation. The cumulus-oocyte complexes (COCs), surrounding an oocyte, play a significant role in oocyte maturation. During this process, when the COCs undergo cumulus expansion wherein tightly compact cumulus cells (CCs) form a dispersed structure, permanent biochemical and molecular modifications occur in the maturing oocytes, indicating that the gene expression between immature and mature oocytes differs significantly. This study focuses on the genes responsible for the cellular components of morphogenesis within the developing oocyte. Brilliant cresyl blue (BCB) was used to determine the developmental capability of porcine oocytes. The immature oocytes (GV stage) were compared with matured oocytes (MII stage), using microarray and qRT-PCR analysis to track changes in the genetic expression profile of transcriptome genes. The data showed substantial upregulation of genes influencing oocyte’s morphology, cellular migration and adhesion, intracellular communication, as well as plasticity of nervous system. Conversely, downregulation involved genes related to microtubule reorganization, regulation of adhesion, proliferation, migration and cell differentiation processes in oocytes. This suggests that most genes recruited in morphogenesis in porcine oocytein vitro,may have cellular maturational capability, since they have a higher level of expression before the oocyte’s matured form. It shows the process of oocyte maturation and developmental capacity is orchestrated by significant cellular modifications during morphogenesis.
APA, Harvard, Vancouver, ISO, and other styles
2

Faizah, Zakiyatul, and Raden Haryanto Aswin. "EXPRESSION OF TRANSFORMING GROWTH FACTOR-beta AND GROWTH DIFFERENTIATION FACTOR-9 ON SHEEP OOCYTES VITRIFIED AFTER AND BEFORE IN VITRO MATURATION." Jurnal Veteriner 22, no. 1 (March 31, 2021): 109–15. http://dx.doi.org/10.19087/jveteriner.2021.22.1.109.

Full text
Abstract:
Oocyte vitrification today became a hope to preserve fertility. Its was a major challenge because of oocyte characteristic in every phase. Immature oocytes were more sensitive to osmotic stress and the membrane wes less stable while mature oocyte have spindles that were very susceptible to temperature decrease. The study aim to compare the effect of vitrification before and after in vitro maturation to the expression TGF beta and GDF9. Oocyte of ewes divided into control groups (K0), K1 maturation prior vitrification, K2 vitrification prior maturation. Vitrification begins with washing oocytes in PBS supplemented of 20%serum for 1-2 minutes, followed by equilibration medium PBS + 20% serum + 10% ethylene glycol for 10-14 minutes, then transferred to 20% serum + PBS + 0.5 M sucrose + 15% ethylene glycol + PROH 15% for 25-30 seconds. Thawing was processed by in the media: 1). PBS + 20% serum + 0.5 M sucrose, 2).PBS + 20% serum + 0.25 M sucrose, and 3).PBS + 20% serum + 0.1 M sucrose. Immunocytochemical stain was performed to evaluate TGF ? and GDF9 expression. Remmele scale index (IRS) was used to read the result. TGF beta expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. GDF9 expression both in oocyte and cummulus of K0 and K1 was significant statistically difference (p<0.05) compare with K2. We concluded that immature oocyte give better expression of TGF â and GDF9 than mature oocyte.
APA, Harvard, Vancouver, ISO, and other styles
3

Moncrieff, Lisa, Ievgeniia Kocherova, Artur Bryja, Wiesława Kranc, Joanna Perek, Magdalena Kulus, Michal Jeseta, et al. "Transcriptomic profile of genes encoding proteins responsible for regulation of cells differentiation and neurogenesis in vivo and in vitro – an oocyte model approach." Medical Journal of Cell Biology 8, no. 1 (April 29, 2020): 1–11. http://dx.doi.org/10.2478/acb-2020-0001.

Full text
Abstract:
AbstractThe growth and development of the oocyte is essential for the ovarian follicle. Cumulus cells (CCs) - a population of granulosa cells - exchange metabolites, proteins and oocyte-derived paracrine factors with the oocyte through gap junctions, to contribute to the competency and health of the oocyte. This bi-directional communication of the cumulus-oocyte complex could be better understood through the micro-analysis of a porcine oocyte gene expression before in vitro maturation (IVM) and after. Additionally, the study of the somatic and gamete cells differentiation capability into neuronal lineage would be promising for future stem cell research as granulosa cells are easily accessible waste material from in vitro fertilization (IVF) procedures. Therefore, in this study, the oocytes of 45 pubertal Landrace gilts were isolated and the protein expression of the COCs were analyzed through micro-analysis techniques. Genes belonging to two ontological groups: neuron differentiation and negative regulation of cell differentiation have been identified which have roles in proliferation, migration and differentiation. Twenty identified porcine oocyte genes (VEGFA, BTG2, MCOLN3, EGR2, TGFBR3, GJA1, FST, CTNNA2, RTN4, MDGA1, KIT, RYK, NOTCH2, RORA, SMAD4, ITGB1, SEMA5A, SMARCA1, WWTR1 and APP) were found to be down-regulated after the transition of IVM compared to in vitro. These results could be applied as gene markers for the proliferation, migration and differentiation occurring in the bi-directional communication between the oocyte and CCs.Running title: Differentiation and neurogenesis in oocyte cells
APA, Harvard, Vancouver, ISO, and other styles
4

Theurkauf, W. E., B. M. Alberts, Y. N. Jan, and T. A. Jongens. "A central role for microtubules in the differentiation of Drosophila oocytes." Development 118, no. 4 (August 1, 1993): 1169–80. http://dx.doi.org/10.1242/dev.118.4.1169.

Full text
Abstract:
Drosophila oocytes develop within cysts containing 16 cells that are interconnected by cytoplasmic bridges. Although the cysts are syncytial, the 16 cells differentiate to form a single oocyte and 15 nurse cells, and several mRNAs that are synthesized in the nurse cells accumulate specifically in the oocyte. To gain insight into the mechanisms that generate the cytoplasmic asymmetry within these cysts, we have examined cytoskeletal organization during oocyte differentiation. Shortly after formation of the 16 cell cysts, a prominent microtubule organizing center (MTOC) is established within the syncytial cytoplasm, and at the time the oocyte is determined, a single microtubule cytoskeleton connects the oocyte with the remaining 15 cells of each cyst. Recessive mutations at the Bicaudal-D (Bic-D) and egalitarian (egl) loci, which block oocyte differentiation, disrupt formation and maintenance of this polarized microtubule cytoskeleton. Microtubule assembly-inhibitors phenocopy these mutations, and prevent oocyte-specific accumulation of oskar, cyclin B and 65F mRNAs. We propose that formation of the polarized microtubule cytoskeleton is required for oocyte differentiation, and that this structure mediates the asymmetric accumulation of mRNAs within the syncytial cysts.
APA, Harvard, Vancouver, ISO, and other styles
5

Dragovic, Rebecca A., Lesley J. Ritter, Samantha J. Schulz, Fred Amato, David T. Armstrong, and Robert B. Gilchrist. "Role of Oocyte-Secreted Growth Differentiation Factor 9 in the Regulation of Mouse Cumulus Expansion." Endocrinology 146, no. 6 (June 1, 2005): 2798–806. http://dx.doi.org/10.1210/en.2005-0098.

Full text
Abstract:
Abstract Oocyte-secreted factors are required for expansion of the mouse cumulus-oocyte complex, which is necessary for ovulation. Oocyte-secreted growth differentiation factor 9 (GDF9) signals through the bone morphogenetic protein receptor II and is currently the primary candidate molecule for the cumulus-expansion enabling factor. This study was conducted to determine whether GDF9 is the mouse cumulus-expansion enabling factor. Cumulus-oocyte complexes were collected from mice, and the oocyte was microsurgically removed to generate an oocytectomized (OOX) complex. OOX complexes treated with FSH alone or recombinant mouse GDF9 alone failed to expand, whereas expansion was induced in the presence of FSH by GDF9, TGFβ1, or coculture with oocytes. A specific GDF9-neutralizing antibody, mAb-GDF9–53, neutralized the expansion of OOX complexes in response to GDF9 but not the expansion of OOX complexes cocultured with oocytes. Using real-time RT-PCR, hyaluronan synthase 2 (HAS2) mRNA expression by OOXs was up-regulated 4- to 6-fold by oocytes and GDF9. Monoclonal neutralizing antibody-GDF9–53 attenuated GDF9-induced OOX HAS2 expression but not oocyte-induced HAS2 expression. A TGFβ antagonist neutralized TGFβ-induced, but not oocyte-induced, expansion of OOX complexes, and when combined with monoclonal neutralizing antibody-GDF9–53 also failed to neutralize oocyte-induced expansion. Furthermore, a soluble portion of the bone morphogenetic protein receptor II extracellular domain, which is a known GDF9 antagonist, completely antagonized GDF9-induced expansion but only partially neutralized oocyte-induced expansion. This study provides further evidence that like TGFβ, GDF9 can enable FSH-induced cumulus expansion, but more importantly, demonstrates that neither GDF9 nor TGFβ alone, nor the two in unison, account for the critical oocyte-secreted factors regulating mouse cumulus expansion.
APA, Harvard, Vancouver, ISO, and other styles
6

Sirard, M. A. "247 THE IMPORTANCE OF FOLLICULAR DIFFERENTIATION TO OBTAIN FULLY COMPETENT BOVINE OOCYTES." Reproduction, Fertility and Development 28, no. 2 (2016): 256. http://dx.doi.org/10.1071/rdv28n2ab247.

Full text
Abstract:
Making an oocyte with the capacity to form a Day-7 embryo that will implant and produce a live healthy calf is a long and complex process. Since the beginning of bovine IVF, it has been clear that the follicle from which an oocyte is obtained is the most important factor to predict outcome. The blastocyst rate is above 75% with in vivo-matured oocytes, but success varies greatly following in vitro maturation, depending more on follicular status than size. Indeed, aspirating oocytes from non-preovulatory follicles can result in 30% blastocyst development following fertilization but rarely more, unless the follicle is selected. Follicles leading to improved development are either early atretic or exposed to FSH withdrawal. If no exogenous FSH is given, natural early atresia will occur the day after the dominant follicle is selected, but will be limited to a few follicles per wave. If FSH is given to generate a wave of dominant follicles, and then withdrawn, most of the follicles will continue growth and differentiation under basal LH and a maximal oocyte quality will be reached between Day 2 and 3 after the last FSH injection. Under these conditions, the oocyte has the right cascade of signaling to condense its chromatin and to prepare for fertilization. Oocytes obtained from less-prepared follicles are of lower quality, as demonstrated by lower blastocyst rates, lower blastocyst quality, lower implantation rates, and higher abortion rates. Genomic analysis of embryos generated from slaughterhouse-derived oocytes indicates a progressive dysfunction in direct link with the in vitro exposure time. The best way to minimize in vitro-related stress is to begin with a competent oocyte and the best demonstration of that is when some animals (treated with FSH and then coasted) generate 100% blastocyst rate for a recovery of more than 5 oocytes, indicating that the initial quality of oocytes is not much affected by the in vitro culture. It is surprising that blastocyst rates following coasting and in vitro maturation can surpass that observed with Day-7 embryo collections based on the number of ovulations. Finally, new epigenetic analysis will progressively unravel differences of oocytes obtained from different sources and will become an important research tool to assess follicular conditions that will lead to minimal culture stress and optimal embryo quality. If epigenetic programming of the embryo depends on its capacity to deal with the stress created by culture, as has been shown in other species, the initial quality of the oocyte will potentially impact the health status of the newborn calf and resulting adult.
APA, Harvard, Vancouver, ISO, and other styles
7

Dragovic, R. A., L. J. Ritter, F. Amato, S. J. Scott, M. Cranfield, N. P. Groome, D. T. Armstrong, and R. B. Gilchrist. "251.Regulation of mouse cumulus expansion by oocyte-secreted growth differentiation factor-9 (GDF-9)." Reproduction, Fertility and Development 16, no. 9 (2004): 251. http://dx.doi.org/10.1071/srb04abs251.

Full text
Abstract:
Oocyte paracrine signalling is necessary for mouse cumulus cell expansion, an important preovulatory process. The oocyte-secreted factor growth differentiation factor-9 (GDF-9) signals through the bone morphogenetic protein receptor-II (BMPR-II) and is currently the primary candidate molecule for the cumulus expansion enabling factor (CEEF). The present study was conducted to determine whether in the mouse GDF-9 is the CEEF. Cumulus oocyte complexes (COC) were collected from eCG-primed mice and the oocyte was microsurgically removed to generate an oocytectomised complex (OOX). An established scoring system was used to measure FSH-induced cumulus expansion; 0 (no expansion) to +4 (maximum expansion). OOX complexes treated with FSH alone failed to expand (score: 0), whereas expansion was significantly (P�<�0.05) induced by either recombinant mouse GDF-9 (score; mean +/– SEM: 2.7 +/– 0.1), recombinant TGF-μ1 (score: 2.6 +/– 0.2) or co-culture with oocytes (score: 2.3 +/– 0.2). A GDF-9 neutralising antibody mAb-53, raised against hGDF-9, was effective in neutralising the response of OOX complexes to GDF-9 (score: 0.1 +/– 0.1), but had no significant effect on the expansion of OOX complexes co-cultured with oocytes (score: 2.3 +/– 0.2). Likewise, a TGF-μ antagonist neutralised (P�<�0.05) TGF-μ-induced, but not oocyte-induced, expansion of OOX complexes. A soluble portion of the BMPR-II ectodomain, a known GDF-9 antagonist, failed to neutralise oocyte-induced cumulus expansion (P�>�0.05) at the highest dose implying that BMPR-II is not a critical receptor involved in regulating cumulus expansion. Using real-time RT-PCR, hyaluronan synthase-2 (HAS2) mRNA expression by OOXs was upregulated 6- to 7-fold by oocytes and GDF-9. The GDF-9 neutralising antibody mAb-53, partially neutralised GDF-9-induced OOX HAS2 expression, but not oocyte-induced HAS2 expression. This study provides evidence that like TGF-μ1, GDF-9 can enable FSH-induced cumulus expansion, however more importantly demonstrates that neither GDF-9 nor TGF-μ1 alone account for the crucial oocyte-secreted factor regulating cumulus expansion in the mouse.
APA, Harvard, Vancouver, ISO, and other styles
8

Guo, Jing, Teng Zhang, Yueshuai Guo, Tao Sun, Hui Li, Xiaoyun Zhang, Hong Yin, et al. "Oocyte stage-specific effects of MTOR determine granulosa cell fate and oocyte quality in mice." Proceedings of the National Academy of Sciences 115, no. 23 (May 21, 2018): E5326—E5333. http://dx.doi.org/10.1073/pnas.1800352115.

Full text
Abstract:
MTOR (mechanistic target of rapamycin) is a widely recognized integrator of signals and pathways key for cellular metabolism, proliferation, and differentiation. Here we show that conditional knockout (cKO) of Mtor in either primordial or growing oocytes caused infertility but differentially affected oocyte quality, granulosa cell fate, and follicular development. cKO of Mtor in nongrowing primordial oocytes caused defective follicular development leading to progressive degeneration of oocytes and loss of granulosa cell identity coincident with the acquisition of immature Sertoli cell-like characteristics. Although Mtor was deleted at the primordial oocyte stage, DNA damage accumulated in oocytes during their later growth, and there was a marked alteration of the transcriptome in the few oocytes that achieved the fully grown stage. Although oocyte quality and fertility were also compromised when Mtor was deleted after oocytes had begun to grow, these occurred without overtly affecting folliculogenesis or the oocyte transcriptome. Nevertheless, there was a significant change in a cohort of proteins in mature oocytes. In particular, down-regulation of PRC1 (protein regulator of cytokinesis 1) impaired completion of the first meiotic division. Therefore, MTOR-dependent pathways in primordial or growing oocytes differentially affected downstream processes including follicular development, sex-specific identity of early granulosa cells, maintenance of oocyte genome integrity, oocyte gene expression, meiosis, and preimplantation developmental competence.
APA, Harvard, Vancouver, ISO, and other styles
9

Uchida, Daisuke, Michiaki Yamashita, Takeshi Kitano, and Taisen Iguchi. "Oocyte apoptosis during the transition from ovary-like tissue to testes during sex differentiation of juvenile zebrafish." Journal of Experimental Biology 205, no. 6 (March 15, 2002): 711–18. http://dx.doi.org/10.1242/jeb.205.6.711.

Full text
Abstract:
SUMMARY Large numbers of apoptotic early diplotene oocytes were observed during the transition from ovary-like undifferentiated gonadal tissue to testes during sex differentiation in presumptive males of the zebrafish (Danio rerio). The percentage of terminal-deoxynucleotidyl-transferase-mediated dUTP nick-end labelling (TUNEL)-positive apoptotic oocytes in the gonads of presumptive males was approximately eight- to 12-fold higher than in genetic all-females. By 29 days post-hatching, all oocytes had disappeared from the gonads of presumptive males. In these males, we also observed apoptotic somatic cells in the ovarian cavity between 23 and 35 days post-hatching. Therefore, the disappearance of oocytes and the decomposition of the ovarian cavity caused by apoptosis during sex differentiation were male-specific events. In genetic all-females, apoptosis in a proportion of early diplotene oocytes was found in the undifferentiated gonads at 15–19 days post-hatching, probably as a result of programmed oocyte loss during ovarian development. These findings suggest that oocyte apoptosis is the mechanism of testicular and ovarian differentiation in zebrafish.
APA, Harvard, Vancouver, ISO, and other styles
10

Chen, Ying, Wendy N. Jefferson, Retha R. Newbold, Elizabeth Padilla-Banks, and Melissa E. Pepling. "Estradiol, Progesterone, and Genistein Inhibit Oocyte Nest Breakdown and Primordial Follicle Assembly in the Neonatal Mouse Ovary in Vitro and in Vivo." Endocrinology 148, no. 8 (August 1, 2007): 3580–90. http://dx.doi.org/10.1210/en.2007-0088.

Full text
Abstract:
In developing mouse ovaries, oocytes develop as clusters of cells called nests or germ cell cysts. Shortly after birth, oocyte nests dissociate and granulosa cells surround individual oocytes forming primordial follicles. At the same time, two thirds of the oocytes die by apoptosis, but the link between oocyte nest breakdown and oocyte death is unclear. Although mechanisms controlling breakdown of nests into individual oocytes and selection of oocytes for survival are currently unknown, steroid hormones may play a role. Treatment of neonatal mice with natural or synthetic estrogens results in abnormal multiple oocyte follicles in adult ovaries. Neonatal genistein treatment inhibits nest breakdown suggesting multiple oocyte follicles are nests that did not break down. Here we investigated the role of estrogen signaling in nest breakdown and oocyte survival. We characterized an ovary organ culture system that recapitulates nest breakdown, reduction in oocyte number, primordial follicle assembly, and follicle growth in vitro. We found that estradiol, progesterone, and genistein inhibit nest breakdown and primordial follicle assembly but have no effect on oocyte number both in organ culture and in vivo. Fetal ovaries, removed from their normal environment of high levels of pregnancy hormones, underwent premature nest breakdown and oocyte loss that was rescued by addition of estradiol or progesterone. Our results implicate hormone signaling in ovarian differentiation with decreased estrogen and progesterone at birth as the primary signal to initiate oocyte nest breakdown and follicle assembly. These findings also provide insight into the mechanism of multiple oocyte follicle formation.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Oocyte differentiation"

1

Dokshin, Gregoriy A. (Gregoriy Aleksandrovich). "Oocyte differentiation is genetically dissociable from the meiotic program in mice." Thesis, Massachusetts Institute of Technology, 2013. http://hdl.handle.net/1721.1/79321.

Full text
Abstract:
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2013.
Cataloged from PDF version of thesis. Page 100 blank.
Includes bibliographical references.
Oogenesis is a developmental program by which a gametogenesis-competent germ cell becomes a fertilization-competent egg. During oogenesis, growth and differentiation of oocytes are closely coordinated with initiation and progression through meiosis. In mammals, the timing of meiotic initiation is sexually dimorphic, with only ovarian and not testicular germ cells initiating meiosis during fetal development. Consequentially, fetal meiotic initiation is thought to be prerequisite to subsequent growth and differentiation of the ovarian germ cell into a fully grown oocyte. Here I present evidence that meiotic initiation and prophase I are genetically separable from oocyte growth and differentiation, thereby, demonstrating that oogenesis consists of two independent processes under separate regulation. This represents a novel view of the oogenesis program and revises the current model of germ cell commitment to oogenesis in mice. The proposed revised model accounts for independent commitment of a germ cell to meiosis and differentiation. This model may provide insights into previously unexplained cases of female infertility and has practical implications for in vitro oogenesis strategies.
by Gregoriy A. Dokshin.
Ph.D.
APA, Harvard, Vancouver, ISO, and other styles
2

GARCIA, BARROS RODRIGO. "DEVELOPMENT OF NEW OOCYTE IN VITRO CULTURE STRATEGIES TO ENHANCE THE OUTCOME OF ASSISTED REPRODUCTIVE TECHNOLOGIES." Doctoral thesis, Università degli Studi di Milano, 2021. http://hdl.handle.net/2434/809746.

Full text
Abstract:
Fertility preservation has received unprecedented attention nowadays. In addition to cryopreservation and re-implantation of embryos, oocytes, and ovarian tissue pieces, in vitro culture system for follicles/oocytes has been considered an alternative strategy for fertility preservation. Reproduction strategies based on the recovery of oocytes' population from antral follicles are unsatisfactory, and the success of this approach has not exceeded 35% of embryos produced in vitro for over 30 years. The possibility of accessing the reserve of smaller follicles (primordial, secondary, and up to the preantral stage) would amplify the number of gametes available for increasing reproductive potential. Furthermore, this would open enormous prospects for the rescue of fertility in various conditions in the human clinic and genetic rescue in animal breeding and biodiversity preservation programs. However, this would require developing protocols capable of growing immature oocytes to the stage in which they can be matured and fertilized in vitro. Culture systems to achieve in vitro growth (IVG) of immature oocytes to maturity and subsequent fertilization in vitro (IVF) have been the subject of research for almost 40 years. Several systems that support the growth of later stages of follicle development from rodents have been developed, with some reporting the production of live young, but they are still at an experimental stage, and further research is required before the protocols could be clinically applied. One of the significant limitations is identifying growth factors, hormones, and nutrients necessary for each specific follicle development stage. This evidence has led to hypothesize the development of culture systems consisting of a step-by-step approach, although no reliable protocols have been developed so far. The oocyte culture at the early stages of development represents an alternative to maximize the potential source of gamete used for fertility preservation. Several attempts have been made to recreate these conditions in vitro, but no reliable protocols have been developed to date. The lack of knowledge in the mechanisms involved in the early development of the oocyte and this passage from growing to fully grown stage be one of the most critical steps during oocyte development, these still represent the significant limiting factor for this technology. The studies conducted during the doctorate program led to defining a physiological culture system that successfully differentiated growing bovine oocytes. This study used parameters predictive of oocyte differentiation to evaluate the current technique's efficiency and efficacy. Based on previous observations from our laboratory, we initially hypothesized that zinc plays a role during the latest stages of oocyte growth and differentiation, particularly in controlling transcription during the final stage of oocyte growth. This first study demonstrated that zinc supplementation improves the meiotic competence of growing oocytes, affects the global transcription activity and the global DNA methylation. This information was used in the next part to better define a culture system for growing oocytes. The subsequent study provided a 5-days protocol named L-IVCO (long in vitro culture of oocytes) to promote growing oocyte differentiation until the acquisition of meiotic and embryonic developmental competencies in a significantly higher proportion of the published protocols. This study demonstrated that a physiological medium could support a gradual transition of the oocyte from immature to mature stage, thus generating suitably quality blastocysts after fertilization. In conclusion, our studies provide an improved protocol that can increase the source of fertilizable gametes in preservation programs and gives a prospective approach in human clinics, animal breeding programs, and salvage intervention of threatened species. Moreover, our studies defined a model to perform in-depth studies of the cellular and molecular processes that regulate the acquisition of meiotic and developmental competence during oocyte differentiation.
APA, Harvard, Vancouver, ISO, and other styles
3

Dhawan, Anil. "The role of oocyte- and embryo-secreted factors in cumulus cell differentiation and their relationship to embryo quality and developmental competence." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0028/MQ52295.pdf.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Bestetti, I. "GENOME WIDE ANALYSIS IN A COHORT OF 46,XX PATIENTS AFFECTED BY AN EXTREME PHENOTYPE OF PRIMARY OVARIAN INSUFFICIENCY: AN EFFICIENT TOOL TO IDENTIFY NEW GENES INVOLVED IN OOCYTE MATURATION AND DIFFERENTIATION." Doctoral thesis, Università degli Studi di Milano, 2016. http://hdl.handle.net/2434/363638.

Full text
Abstract:
Primary Ovarian Insufficiency (POI) is a heterogeneous group of disorders with an incidence of 1:10,000 women by age 20, 1:1,000 by age 30, 1:100 by age 40. POI describes the progression toward the cessation of ovarian function and can occur in the most serious form as primary amenorrhea (PA), with absent pubertal development and/or ovarian dysgenesis (OD), or in milder phenotype with post-pubertal onset and secondary amenorrhea (SA). Several are the etiological causes that may induce ovarian dysfunction, among which the genetic component is considered prevalent (as supported by the occurrence of families with more than one affected women and the existence of several idiopathic cases) but highly heterogeneous. 46,XX non-syndromic women showing the most severe phenotype, characterized by the absence of pubertal development with PA and OD, are very rare but the search for genetic variations in this extreme phenotype may be more effective in identifying novel pathogenic mechanisms. Hence, using high resolution array-CGH we searched for rare high-penetrant CNVs involving genes essential for ovarian function in a cohort of 67 46,XX non-syndromic patients affected by PA, namely 53 sporadic (79.1%) and 14 familial (20.9%) cases. 28 out of 67 women resulted positive to array-CGH analysis because having at least one rare “ovarian” CNV: a total number of 32 CNVs involving 37 ovarian genes was selected. Population from Database of Genomic Variants (DGV) was used to evaluate the rarity of POI CNVs, but it does not match to the ideal set of controls for POI disease (neither age nor gender of DGV controls are known). Thus, to better understand the CNVs contribution in disease onset, the rare “ovarian” CNVs found in patients according to DGV were searched in an ad hoc control cohort, previously screened by array-CGH, consisting in 140 healthy women with normal reproductive life and physiological menopause. 28 out of 32 rare “ovarian” CNVs detected in patients were not found in the control group thus supporting their role in the POI’s pathogenesis. Moreover, to evaluate the presence of a significant enrichment in ovarian genes in the POI group, array-CGH of the ad hoc control cohort were analyzed with the same approach adopted for patients cohort and 49 CNVs involving 54 ovarian genes were selected. Several statistical analyses were performed comparing patients’ to controls’ data and revealed no significant differences. Nevertheless, the CNVs found in the POI cohort containing ovarian genes are more harmful respect to the CNVs identified in the controls cohort. The 37 genes perturbed or possibly perturbed by POI CNVs are implicated in several ovarian processes (e.g., regulation of cytoskeleton dynamics for oocytes asymmetric division, maintenance of oocytes genomic integrity, ovarian differentiation, follicular development, and meiotic resumption), thus supporting their involvement in POI etiology. Validation and characterization of selected CNVs, as well as the study of a possible gene perturbation at mRNA level, was also crucial in order to perform a correct genotype-phenotype correlation and to propose new candidate genes for POI disease (e.g. TP63, VLDLR). 39 out of 67 women resulted negative for rare “ovarian” CNVs (58.2%) suggesting to combine different genomic approaches in order to increase the detection rate of the disorder. Hence, 17 out of 67 collected patients, were submitted to a preliminary WES analysis searching for rare SNVs in a total of 191 genes selected from array-CGH data, and literature regarding POI and ovary. The WES preliminary analysis allowed to confirm the importance of some array CGH new candidate genes in POI onset (e.g. VLDLR) and the complex heterogeneity of POI. The combination of these molecular evidences, with major or minor contribution, might have been at the basis of POI supporting the existence of a disease genetic model characterized by oligogenic heterozygosity (i.e., the simultaneous presence in a single patient of multiple heterozygous quantitative variants/rare mutations, both de novo and/or inherited, affecting multiple genes). The present approach using both array-CGH and WES techniques, resulted an efficient tool to identify rare variants (CNVs and SNVs) involving both genes already reported in POI, and new candidate genes with a role in oocyte maturation and differentiation. The results of this study are promising to expand the knowledge about the molecular pathways involved in POI pathogenesis and probably provide the basis for a more accurate genetic diagnosis of POI patients.
APA, Harvard, Vancouver, ISO, and other styles
5

Nasseri, Roksana. "Transcriptional activity of sex chromosomes in the oocytes of the B6.Ytir sex-reversed female mouse." Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21613.

Full text
Abstract:
In the B6.YTIR mouse strain, half of the XY progeny develop bilateral ovaries and the female phenotype. These XY females are infertile mainly due to the death of their embryos. This developmental failure has been attributed to a defect intrinsic to the XY oocyte.
The present study examined the transcriptional activity of the X and Y chromosomes in these oocytes. RT-PCR results show that the Ube1y gene is transcribed in the XY ovary at all stages examined and also in growing XY oocytes. The Sry gene was transcribed only at the onset of ovarian differentiation whereas the Zfy gene was undetectable at all stages during fetal life. The Xist gene, which is involved in X inactivation, was not expressed in XY oocytes. We speculate that expression of Y-encoded genes may have a deleterious effect on the quality of the oocytes and thus renders them incompetent for post-fertilization development.
APA, Harvard, Vancouver, ISO, and other styles
6

Bennett, Paul Edward Jr. "A proteomics approach: Identification of proteins mediating different steps in the specification and differentiation of Drosophila melanogaster oocytes." Diss., Connect to online resource, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3239458.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

MARO, BERNARD. "Etudes sur l'organisation et le role du cytosquelette lors du developpement precoce de la souris." Paris 7, 1988. http://www.theses.fr/1988PA077223.

Full text
Abstract:
Etude de la mise en place des asymetries permettant la naissance de 2 types cellulaires distincts apres une division inegale, a la fois lors de l'expulsion du deuxieme globule polaire (differenciation terminale de l'ovocyte) et lors de la divergence des deux premieres populations cellulaires apres compaction de l'embryon de souris. Mise en evidence du role des chromosomes dans la formation du globule polaire
APA, Harvard, Vancouver, ISO, and other styles
8

Fitzpatrick, Martin S. "Endocrine regulation of final oocyte maturation and sex differentiation in salmonids." Thesis, 1990. http://hdl.handle.net/1957/37097.

Full text
Abstract:
Sexual maturation and sex differentiation comprise facets of a common theme: reproduction. The endocrine system regulates many of the critical physiological processes necessary for reproduction and offers a framework within which technologies can be developed for controlling sexual maturation and sex differentiation. The studies described in this thesis were undertaken to improve the understanding of the endocrine control of these critical stages of development in salmonids. Final ovarian maturation in salmon is accompanied by dynamic changes in plasma hormone levels. Ovulation can be accelerated through the use of hormones such as gonadotropin releasing hormone or its analogs (GnRHa). The effectiveness of GnRHa often depends on the timing of treatment. To determine if plasma concentrations of steroids can be used to predict the sensitivity of adult female coho salmon (Oncorhynchus kisutch) to GnRHa, circulating levels of testosterone, 17α,20β-dihydroxyprogesterone (DHP), and estradiol were measured before and after injection with GnRHa to accelerate ovulation. We found that high levels of testosterone were predictive of early response of coho salmon to GnRHa treatment. The correlation between testosterone and ovulatory response to GnRHa suggested a possible functional relation. However. implantation or injection of testosterone. 17α-methyltestosterone (MT), or the antiandrogen, cyproterone acetate (CA), before or with GnRHa treatment did not affect the ovulatory response of coho or chinook salmon ( 0. tshawytscha) to GnRHa. Chinook salmon treated with MT alone had accelerated ovulation in comparison to controls. If steroids are involved in sex differentiation. steroids must be produced early in development. In vitro production of steroids in both coho salmon and rainbow trout (0. mykiss) was assessed from hatch through sex differentiation. Cortisol, androstenedione, testosterone, and estradiol were produced just after hatching by tissue explants that contained anterior kidneys and gonads of coho salmon. To circumvent the problem of not knowing the sex of individuals until after sex differentiation, single-sex populations of rainbow trout were produced by gynogenesis or androgenesis. Tissue explants produced more androstenedione than testosterone or estradiol. More androgens were produced by testes and more estradiol was produced by ovaries within 6 to 10 weeks of hatching. Dietary treatment with estradiol or MT inhibited gonadal steroid secretion. Electrophoresis of gonadal homogenates from salmonids revealed several sex-specific bands. In particular, a prominent band of about 50,000 daltons was apparent in ovaries but not testes. Production of sex-specific proteins may be affected by dietary steroid treatment.
Graduation date: 1991
APA, Harvard, Vancouver, ISO, and other styles

Books on the topic "Oocyte differentiation"

1

Fitzpatrick, Martin S. Endocrine regulation of final oocyte maturation and sex differentiation in salmonids. 1990.

Find full text
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Oocyte differentiation"

1

Clapp, Mara, and Florence L. Marlow. "Acquisition of Oocyte Polarity." In Results and Problems in Cell Differentiation, 71–102. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-60855-6_4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Yeste, Marc, Celine Jones, Siti Nornadhirah Amdani, and Kevin Coward. "Oocyte Activation and Fertilisation: Crucial Contributors from the Sperm and Oocyte." In Results and Problems in Cell Differentiation, 213–39. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-44820-6_8.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Menn, Francoise Le, Joan Cerdà, and Patrick J. Babin. "Ultrastructural aspects of the ontogeny and differentiation of ray-finned fish ovarian follicles." In The Fish Oocyte, 1–37. Dordrecht: Springer Netherlands, 2007. http://dx.doi.org/10.1007/978-1-4020-6235-3_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Oh, Denise, and Douglas W. Houston. "RNA Localization in the Vertebrate Oocyte: Establishment of Oocyte Polarity and Localized mRNA Assemblages." In Results and Problems in Cell Differentiation, 189–208. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-60855-6_9.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Susor, Andrej, and Michal Kubelka. "Translational Regulation in the Mammalian Oocyte." In Results and Problems in Cell Differentiation, 257–95. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-60855-6_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Kloc, Malgorzata, Rafik M. Ghobrial, Ewa Borsuk, and Jacek Z. Kubiak. "Polarity and Asymmetry During Mouse Oogenesis and Oocyte Maturation." In Results and Problems in Cell Differentiation, 23–44. Berlin, Heidelberg: Springer Berlin Heidelberg, 2012. http://dx.doi.org/10.1007/978-3-642-30406-4_2.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Chaigne, Agathe, Marie-Emilie Terret, and Marie-Hélène Verlhac. "Asymmetries and Symmetries in the Mouse Oocyte and Zygote." In Results and Problems in Cell Differentiation, 285–99. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-53150-2_13.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Kaláb, Petr, Petr Šolc, and Jan Motlík. "The Role of RanGTP Gradient in Vertebrate Oocyte Maturation." In Results and Problems in Cell Differentiation, 235–67. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-19065-0_12.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Reichman, Rachel, Benjamin Alleva, and Sarit Smolikove. "Prophase I: Preparing Chromosomes for Segregation in the Developing Oocyte." In Results and Problems in Cell Differentiation, 125–73. Cham: Springer International Publishing, 2017. http://dx.doi.org/10.1007/978-3-319-44820-6_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Stricker, Stephen A. "Marine Nemertean Worms for Studies of Oocyte Maturation and Aging." In Results and Problems in Cell Differentiation, 3–14. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-92486-1_1.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "Oocyte differentiation"

1

Ahmadian, M. T., G. R. Vossoughi, A. A. Abbasi, and P. Raeissi. "Modeling of Cell Deformation Under External Force Using Neural Network." In ASME 2010 International Mechanical Engineering Congress and Exposition. ASMEDC, 2010. http://dx.doi.org/10.1115/imece2010-38056.

Full text
Abstract:
Embryogenesis, regeneration and cell differentiation in microbiological entities are influenced by mechanical forces. Therefore, development of mechanical properties of these materials is important. Neural network technique is a useful method which can be used to obtain cell deformation by the means of force-geometric deformation data or vice versa. Prior to insertion in the needle injection process, deformation and geometry of cell under external point-load is a key element to understand the interaction between cell and needle. In this paper the goal is the prediction of cell membrane deformation under a certain force, and to visually estimate the force of indentation on the membrane from membrane geometries. The neural network input and output parameters are associated to a three dimensional model without the assumption of the adherent affects. The neural network is modeled by applying error back propagation algorithm. In order to validate the strength of the developed neural network model, the results are compared with the experimental data on mouse oocyte and mouse embryos that are captured from literature. The results of the modeling match nicely the experimental findings.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Oocyte differentiation' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography