Journal articles: 'Onesteel'

1

Garlick, C., M. Griffiths, P. Whitehouse, and C. Gore. "Inclusion engineering: a keytool in achieving steelmaking operations stability at OneSteel Whyalla." Ironmaking & Steelmaking 29, no. 2 (April 2002): 140–46. http://dx.doi.org/10.1179/030192302225004098.

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2

Brunner, M. "Development and Deployment of a Data Capture and Reporting System for Onesteel Wire Products." Australian Journal of Multi-Disciplinary Engineering 11, no. 1 (January 2014): 81–90. http://dx.doi.org/10.7158/14488388.2014.11464885.

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3

Pramod, K. V., and Dr B. R. Patagundi. "Plastic Section Moduli for I.S. Rolled Steel Beam Sections Zpy about Y-Y Axis." International Journal for Research in Applied Science and Engineering Technology 10, no. 12 (December 31, 2022): 1979–84. http://dx.doi.org/10.22214/ijraset.2022.48395.

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Abstract: There are many situations in which the I-Sections used in construction are subjected to moments about their weaker axis, i.e. the y-y axis. For such purposes the Plastic Section Modulus about y-y axis becomes necessary. In the present paper an attempt has been made to calculate and present the values of Zpy, for I.S. Rolled Steel Beam Sections (with tapered flanges). Since IS 800 : 2007[2] has not given the values of Zpy, of any section, one tapered flange I-Section, viz., 125 TFB @ 13.1 kg/m, from “Onesteel” (Australia),[4] has been used to ascertain the correctness of calculations. The results are presented in table form in descending order.

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Prestidge, John, Mark Lee, and Louis Wibberley. "EAF Process Monitoring at OneSteel's Sydney Steel Mill." Steel Research 73, no. 1 (January 2002): 5–8. http://dx.doi.org/10.1002/srin.200200165.

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5

Brouste, Alexandre, Christophe Dutang, and Darel,Noutsa Mieniedou. "OneStep : Le Cam's One-step Estimation Procedure." R Journal 13, no. 1 (2021): 366. http://dx.doi.org/10.32614/rj-2021-044.

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6

Marcinkowski, A., and B. Mariański. "Are low-energy direct reactions mainly onestep?" Physics Letters B 433, no. 3-4 (August 1998): 223–28. http://dx.doi.org/10.1016/s0370-2693(98)00725-4.

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Maksimenko, S. V., E. S. Savchenko, and N. S. Ogneva. "Cultivation of mice embryos on various nutrient media up to the stage of two blastomers after the microinjection of a genetically-engineered construct." Journal Biomed 17, no. 3E (October 26, 2021): 53–58. http://dx.doi.org/10.33647/2713-0428-17-3e-53-58.

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The normal growth and differentiation of mammalian embryos in vitro during the pre-implantation period depends on the availability of appropriate metabolic substrates. Selection of optimal cultivation conditions when working with microinjected embryos is of significance, since any manipulations can reduce their viability. This is particularly important when obtaining transgenic animals. In order to determine the most effective culture medium, we studied the survival of mouse embryos after the microinjection of a genetically engineered construct into the pronuclei and further cultivation on the M16 and Onestep nutrient media. Compared to the M16 medium, an increase in the number of embryos that developed to the stage of two blastomeres on the Onestep medium was observed. Differences were also revealed in the two control groups, the embryos in which were not subjected to microinjection, but were cultured on the same media under the same conditions as the embryos of the experimental groups. We found that the Onestep medium, in which the percentage of survived embryos exceeded that in the M16 medium, is a more efficient medium for the cultivation of intact embryos.

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Marassi, M. A., O. A. Bovo, G. L. Lavia, and L. A. Mroginski. "Regeneration of rice double haploids using a onestep culture procedure." Journal of Plant Physiology 141, no. 5 (May 1993): 610–14. http://dx.doi.org/10.1016/s0176-1617(11)80464-5.

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9

Vickers, Ivan, Kevin Harvey, Kereann Nelson, Michelle Brown, Marion Bullock-DuCasse, and John Lindo. "Evaluation of OneStep Dengue NS1 RapiDip™ InstaTest and OneStep Dengue Fever IgG/IgM RapiCard™ InstaTest during the course of a dengue type 1 epidemic." Diagnostic Microbiology and Infectious Disease 89, no. 4 (December 2017): 271–75. http://dx.doi.org/10.1016/j.diagmicrobio.2017.08.019.

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10

Pillai, Krishnakumar. "Single crystalline rutile TiO2 nanorods synthesis by onestep catalyst-free vapor transport method." Solid State Communications 333 (July 2021): 114342. http://dx.doi.org/10.1016/j.ssc.2021.114342.

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11

Rozanski, Gabriela, and David Putrino. "Recording context matters: Differences in gait parameters collected by the OneStep smartphone application." Clinical Biomechanics 99 (October 2022): 105755. http://dx.doi.org/10.1016/j.clinbiomech.2022.105755.

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12

Ayryan, E. A., M. Hnatic, and V. B. Malyutin. "On the equivalence of operator and combinatorial approaches for onestep random Markov processes." Proceedings of the National Academy of Sciences of Belarus. Physics and Mathematics Series 58, no. 1 (April 4, 2022): 21–33. http://dx.doi.org/10.29235/1561-2430-2022-58-1-21-33.

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Herein, for one-step random Markov processes the comparison of the operator and combinatorial methods based on the use of functional integrals is performed. With the combinatorial approach, the transition from the stochastic differential equation to the functional integral is used. This allows us to obtain the expression for the mean population size in terms of the functional integral. With the operator approach, the transition to the functional integral is performed via the creation and annihilation operators. It is shown that the mean values calculated using the functional integrals arising in the combinatorial and operator approaches coincide.

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13

Marvez-Valls, E. G. "OneStep ABAcard p30 Test for the Identification of Semen in Sexual Assault Victims." Academic Emergency Medicine 10, no. 5 (May 1, 2003): 564. http://dx.doi.org/10.1197/aemj.10.5.564.

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14

Kearsey, J., H. Louie, and H. Poon. "Validation Study of the “Onestep ABAcard® PSA Test” Kit for RCMP Casework." Canadian Society of Forensic Science Journal 34, no. 2 (January 2001): 63–72. http://dx.doi.org/10.1080/00085030.2001.10757518.

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15

Alfieri, Angelo, George Crawford, and Ijaz Ahmad. "One-Step Sample Preparation Technique for Broad Spectrum Gas Chromatographic/Mass Spectrometric Determination of Organic Priority Pollutants in Water." Journal of AOAC INTERNATIONAL 72, no. 5 (September 1, 1989): 760–65. http://dx.doi.org/10.1093/jaoac/72.5.760.

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Abstract A rapid and cost-effective sample preparation technique is described for the qualitative and quantitative determination of a broad spectrum of organic contaminants in water. The technique involves simultaneous in-situ acetylation of phenols with acetic anhydride and onestep extraction of phenol acetates and basic and neutral organic compounds from water. The extract is concentrated and analyzed by gas chromatography/mass spectrometry. The recovery and precision data of selected base, neutral, and phenolic compounds are given. The advantages and disadvantages of the in-situ acetylation/extraction technique are discussed

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Wang, Xue Yi, Zai Xiang Zheng, Hui Shen, and Wei Wei Zhang. "Comparative Analysis of Onestep and Multistep Forming in Automotive I/P Beam Hydroforming Process." Applied Mechanics and Materials 395-396 (September 2013): 962–65. http://dx.doi.org/10.4028/www.scientific.net/amm.395-396.962.

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After a brief introduction of state of art of hydraulic forming technology in China and abroad, the simulation experiment and finite element model of the directly forming and step-by-step forming are designed. According to the different characteristics of forming and spring back, the simulation analysis are performed through dynamic display algorithm and statistic implicit algorithm, and analyze the influence on tube thickness, spring back amount and the final residual stress of two different manufacturing processes which is compared with the experimental result. The comparison result can provide some theoretical reference for dashboard beam hydraulic forming process design, process parameter setting and mold design.

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17

Oosterwijk, J. C., W. E. Mesker, M. C. M. Ouwerkerk, C. F. H. M. Knepflé, M. J. M. van der Burg, C. C. Wiesmeijer, G. C. Beverstock, et al. "Detection of fetal erythroblasts in maternal blood by onestep gradient enrichment and immunocytochemical recognition." Early Human Development 47 (December 1996): S95—S97. http://dx.doi.org/10.1016/s0378-3782(96)01830-0.

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18

Sutcharit, Chirasak, Thierry Backeljau, and Somsak Panha. "Re-description of the type species of the genera Ganesella Blanford, 1863 and Globotrochus Haas, 1935; with description of a new Ganesella species from Thailand (Eupulmonata, Camaenidae)." ZooKeys 870 (August 7, 2019): 51–76. http://dx.doi.org/10.3897/zookeys.870.36970.

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The taxonomy of the speciose genus Ganesella W.T. Blanford, 1863 and the endemic genus Globotrochus Haas, 1935 is unclear since the anatomical characters of the the type species of these two genera have never been reported before. Therefore, the present paper provides the first anatomical descriptions of the reproductive apparatus, pallial system and radula of Helix capitium Benson, 1848 and Helix onestera Mabille, 1887, the respective type species of Ganesella and Globotrochus. In addition, Ganesella rhombostoma (Pfeiffer, 1861) and Ganesella carinella (Möllendorff, 1902) from Thailand are re-described, and a new species, Ganesella halabalah Sutcharit & Panha, sp. nov., from southern Thailand is described. This new species differs from all others by having a larger shell, an obtuse apex and an aperture lip with a prominent beak-like deflection.

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19

Scott, Virginia. "La Virtu et la volupté. Models for the Actress in Early Modern Italy and France." Theatre Research International 23, no. 2 (1998): 152–58. http://dx.doi.org/10.1017/s0307883300018496.

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In 1982 Ferdinando Taviani proposed in Il segreto della commedia dell'arte that the earliest known actresses in Italy may have been drawn from the ranks of the courtesans, the oneste meretrici, forced from Rome after the Council of Trent (1545–63) by papal reforming zeal. This hypothesis has been affirmed by several other scholars in Europe and the United States and is becoming widely accepted not just as a feasible theory but as gospel, the exclusive conduit through which women entered the western theatre.

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20

Zheng, Shuhai, and Vladimir Novotny. "Stochastic Modeling of Combined Sewer Flows." Water Science and Technology 24, no. 6 (September 1, 1991): 35–40. http://dx.doi.org/10.2166/wst.1991.0138.

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Modeling of sewer flow and quality is essential for real-time control (RTC) of sewer system and minimization of combined sewer overflows (CSOs). In this paper, the Box-Jenkins type transfer function is applied to the daily rainfall and sewer flow data to incorporate the stochastic nature of the rainfall and flow series. Two system identification techniques, different from the traditional Box-Jenkins', approach, are developed to identify sewer flow models. Both techniques are illustrated through their application of modeling sewer flow with actual time series of flow and rainfall from a combined sewer system. The onestep ahead predicted flow values are found to be in good agreement with the measured values.

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21

Asadi, B., and F. Seyedasgari. "67 The growth and development of invivo-derived dromedary embryos during short-term incubation: Use of embryo holding medium and the effect of embryonic morphology." Reproduction, Fertility and Development 33, no. 2 (2021): 140. http://dx.doi.org/10.1071/rdv33n2ab67.

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Production of invivo embryos for transfer in dromedary camel is a well-established practice, whereas freezing of these embryos is still an ongoing challenge. A common approach in evaluation of freeze–thawing method is achieved by studying invitro development of frozen–thawed embryos. However, not much is known about the development pattern of fresh dromedary embryos during incubation. The objectives of this study were to evaluate the usefulness of commercial holding media for easy short-term culture of these embryos and to provide preliminary insights on the growth and development of hatched blastocysts with different shapes. Recovered hatched blastocysts from superovulated donors were graded as transferable and non-transferable. Embryos with significant folding or crinkliness were further categorized as collapsed, whereas those with a round or oval appearance were categorized as spherical. Culture was performed in 500-μL drops at 38.5°C, 5% O2, 0–6% CO2, and maximum humidity in groups of 2 to 4. The 4 experimental media included culture medium (CM; TCM-199, 10% fetal calf serum (FCS), 0.3mM sodium pyruvate, 2.2mg mL−1 sodium bicarbonate), serum-supplemented holding medium (SSH; Syngro+10% FCS), serum-free holding medium (Syngro) and V-Onestep (Vitromed). In experiment 1, a total of 36 embryos were assigned to 4 groups and further development of the embryos was monitored up to 96h by morphological evaluations, identifying static and degenerating embryos on daily basis. In experiment 2, a total of 16 spherical and 16 collapsed embryos were cultured in SSH and CM and two-thirds of the culture drop was replaced with fresh medium at 72h. The proportion of developing embryos and their size expansion was compared between treatments by Fisher’s test and Mann–Whitney U test, respectively. Statistically similar proportions of embryos continued to develop in all media within the first 48h despite a numeric advantage in CM group; at 72h, the proportion of growing embryos was significantly higher in CM (77.8%) and SSH (66.6%) compared with SFH (11.1%) and OneStep (22.2%) (P<0.05). None of the embryos in SFH and only 1 embryo in the V-Onestep group survived beyond 72h, whereas 3/9 embryos in SSH and 7/9 embryos in CM continued to expand. In experiment 2, the proportion of spherical embryos that developed was higher compared with their collapsed counterparts (8/8 in both groups vs. 5/8 and 4/8 in CM and SSH, respectively) at 24h. However, remaining collapsed embryos grew and expanded at similar rates to spherical ones in each group (P>0.05). Replacing the medium did not favour continuation of embryonic growth in SSH beyond 72 h; only 5/16 embryos survived to 96h compared with 12/16 in CM. In conclusion, serum-supplemented commercial holding preparations provide comparable results to culture medium for short-term incubation of invivo dromedary embryos. Natural collapsing of hatched blastocysts might be associated with lower developmental competence.

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22

Asadi, B., and F. Seyedasgari. "67 The growth and development of invivo-derived dromedary embryos during short-term incubation: Use of embryo holding medium and the effect of embryonic morphology." Reproduction, Fertility and Development 33, no. 2 (2021): 140. http://dx.doi.org/10.1071/rdv33n2ab67.

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Production of invivo embryos for transfer in dromedary camel is a well-established practice, whereas freezing of these embryos is still an ongoing challenge. A common approach in evaluation of freeze–thawing method is achieved by studying invitro development of frozen–thawed embryos. However, not much is known about the development pattern of fresh dromedary embryos during incubation. The objectives of this study were to evaluate the usefulness of commercial holding media for easy short-term culture of these embryos and to provide preliminary insights on the growth and development of hatched blastocysts with different shapes. Recovered hatched blastocysts from superovulated donors were graded as transferable and non-transferable. Embryos with significant folding or crinkliness were further categorized as collapsed, whereas those with a round or oval appearance were categorized as spherical. Culture was performed in 500-μL drops at 38.5°C, 5% O2, 0–6% CO2, and maximum humidity in groups of 2 to 4. The 4 experimental media included culture medium (CM; TCM-199, 10% fetal calf serum (FCS), 0.3mM sodium pyruvate, 2.2mg mL−1 sodium bicarbonate), serum-supplemented holding medium (SSH; Syngro+10% FCS), serum-free holding medium (Syngro) and V-Onestep (Vitromed). In experiment 1, a total of 36 embryos were assigned to 4 groups and further development of the embryos was monitored up to 96h by morphological evaluations, identifying static and degenerating embryos on daily basis. In experiment 2, a total of 16 spherical and 16 collapsed embryos were cultured in SSH and CM and two-thirds of the culture drop was replaced with fresh medium at 72h. The proportion of developing embryos and their size expansion was compared between treatments by Fisher’s test and Mann–Whitney U test, respectively. Statistically similar proportions of embryos continued to develop in all media within the first 48h despite a numeric advantage in CM group; at 72h, the proportion of growing embryos was significantly higher in CM (77.8%) and SSH (66.6%) compared with SFH (11.1%) and OneStep (22.2%) (P<0.05). None of the embryos in SFH and only 1 embryo in the V-Onestep group survived beyond 72h, whereas 3/9 embryos in SSH and 7/9 embryos in CM continued to expand. In experiment 2, the proportion of spherical embryos that developed was higher compared with their collapsed counterparts (8/8 in both groups vs. 5/8 and 4/8 in CM and SSH, respectively) at 24h. However, remaining collapsed embryos grew and expanded at similar rates to spherical ones in each group (P>0.05). Replacing the medium did not favour continuation of embryonic growth in SSH beyond 72 h; only 5/16 embryos survived to 96h compared with 12/16 in CM. In conclusion, serum-supplemented commercial holding preparations provide comparable results to culture medium for short-term incubation of invivo dromedary embryos. Natural collapsing of hatched blastocysts might be associated with lower developmental competence.

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23

Wang, Bin Bin, Bing Bing Fan, Wen Li, Chen Yang Wang, Bing Sun, Biao Zhao, Xiao Xuan Pian, Hao Chen, and Rui Zhang. "Effect of Ni Coated SiC on Mechanical Properties of SiC/Fe Composites." Key Engineering Materials 602-603 (March 2014): 578–81. http://dx.doi.org/10.4028/www.scientific.net/kem.602-603.578.

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A facile onestep in situ chemical deposition method was introduced to synthesize Ni coated SiC composite powders, which used NiCl2·6H2O as nickel source and N2H4·H2O as reductant, respectively. SiC(Ni)/Fe composites were prepared by current-vacuum hot-pressed sintering at 850°C, 40MPa for 30min. Scanning electronic microscope (SEM) and X-ray diffraction (XRD) were used to characterize the structure, morphology and composition of the prepared Ni/SiC core/shell composite powders. It is found that the Ni coated SiC is favorable to improve the dispersion and compatibility of SiC(Ni)/ Fe composites. By a series of contrast experiments, it is shown that the sintering sample with 5wt% of SiC(Ni) has the best mechanical properties. The relative density, Vickers hardness and bending strength is 91.67%, 4.72GPa and 508MPa, respectively.

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24

Nguyen, Thanh Thi Thai, Huy Hong Tran, Han Hoai Tran, Viet Van Pham, Hieu Van Le, and Thi Minh Cao. "Synthesis and survey of the photocatalytic activity of SnO2/TiO2 nanotubes material under visible light." Science and Technology Development Journal - Natural Sciences 1, T4 (December 31, 2017): 149–56. http://dx.doi.org/10.32508/stdjns.v1it4.499.

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In this paper, SnO2 nano particles were loaded on TiO2 nanotubes (SnO2/TNTs) by onestep hydrothermal method with the purpose to enhance the photocatalytic ability of TiO2 nanotubes (TNTs). Structure and morphology of the material were characterized by X-ray diffraction measurement (XRD) and transmission electron microscopy (TEM). SnO2 nano particles onto TNTs prevent the recombination of electronhole pairs at the interface area. This contribution increased the photocatalytic efficiency of the material. The effect of SnO2 contents on the photocatalytic activities of the composite materials was also investigated with methylene blue that is chosen as the pollutant in industry. The results showed that SnO2/TNTs composite materials had been successfully synthesized by one-step hydrothermal method and SnO2/TNTs composite material with 2 % SnO2 content loading possesses the highest photocatalytic efficiency with 70 % after 180 minutes illuminated under sunlight.

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25

Özcan, Işıl, Aslıhan Köroğlu, Ediz Kale, Tuncer Özçelik, and Burak Yilmaz. "Clinical evaluation of one-step impression technique and definitive casts." Balkan Journal of Dental Medicine 26, no. 2 (2022): 82–87. http://dx.doi.org/10.5937/bjdm2202082q.

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Background/Aim: The purpose of this study was to evaluate the quality of impressions made with vinyl polysiloxane (VPS) material in clinical conditions by using the one-step impression technique, and the quality of casts generated from these impressions. The effect of operator, number and location of abutments, and presence of bleeding were also investigated. Material and Methods: A total of 150 fixed dental prosthesis (FDP) impressions were taken and considered acceptable by 3 experienced prosthodontists in a clinic in an institutional setting. The impressions were evaluated and rated by another experienced prosthodontist and respective casts were evaluated and rated by an experienced dental technician using a digital microscope with ×200 magnification. The defects observed were noted as bubbles, voids, tears, or other defects. A scale was structured for the impressions and casts with ratings of Alpha (excellent; no defects), Bravo (acceptable; small defects), Charlie (inadequate; defects that require remaking of impression), and Delta (unacceptable; substantial defects at preparation finish lines). The data were analyzed with the Chi-square test for inter-operator, number of abutments, and location of prepared tooth variables (a=0.05). Results: The scale ratings were 85 Alpha (57%), 52 Bravo (34%), 6 Charlie (4%), and 7 Delta (5%) for the impressions, and 81 Alpha (54%), 58 Bravo (39%), 4 Charlie (3%), and 7 Delta (5%) for the respective casts. Sixty-nine percent of the impressions and respective casts were rated with the same score. The scale rating results were not influenced by inter-operator variability or number of abutments. Location of the prepared tooth was significant for anterior/posterior (p=0.04), but was not significant for maxilla/ mandible (p>0.05). Bleeding at the preparation site had a significant effect on the acceptability of the impression (p=0.003). Conclusions: The acceptability of VPS impressions using onestep technique was independent of the operator, number of abutments, or whether the prepared tooth was in maxilla or mandible. Impressions of teeth in the anterior region were more acceptable than those located in the posterior. Bleeding negatively affected the acceptability of the impressions. Clinicians may use VPS impressions for the fabrication of FDPs using onestep dual-phase technique. However, clinicians should carefully evaluate their impressions when bleeding is present when using this technique.

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Ortega-Requena, S., M. Serrano-Arnaldos, M. C. Montiel, M. F. Máximo, J. Bastida, and M. D. Murcia Almagro. "Biocatalytic Synthesis of Polymeric Esters Used as Emulsifiers." Chemical & biochemical engineering quarterly 33, no. 1 (2019): 79–86. http://dx.doi.org/10.15255/cabeq.2018.1303.

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Polyglycerol polyricinoleate (PGPR) is a polymeric ester widely used as emulsifier in the food industry. In this work, PGPR biocatalytic synthesis was carried out in a onestep solvent-free enzymatic process using lipase CALB immobilized in Lewatit® Monoplus MP 64 by adsorption. The optimal immobilization conditions were determined: initial enzyme concentration of 13 mg of Lowry protein per mL phosphate buffer pH 7, and ricinoleic acid as a support activator. An immobilized derivative with 35.93 ± 4.90 mg of Lowry protein per g of dry support was obtained. It was used as a catalyst for PGPR production in open air and vacuum batch reactors, and the results obtained showed that only when the reaction equilibrium was shifted towards ester production by means of water removal, the PGPR produced fulfilled the European legislation (acid value ≤ 6 mg of KOH per g of product).

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Nunes, Silvia Regina Nunes. "Textualidade infográfica eletrônica: efeitos de velocidade para a leitura." RUA 20, no. 1 (July 3, 2015): 6. http://dx.doi.org/10.20396/rua.v20i1.8637534.

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Neste trabalho, abordo o modo como a injunção ao clicar produz trajetos de leitura no infográfico eletrônico e como esses trajetos são tecidos por meio da reiteração entre formulações verbal-visual e visual-visual. Apresento o modo como a textualidade infográfica eletrônica produz a estereotipia e a contenção de sentidos ao funcionar sob oNeste trabalho, abordo o modo como a injunção ao clicar produz trajetos de leitura no infográfico eletrônico e como esses trajetos são tecidos por meio da reiteração entre formulações verbal-visual e visual-visual. Apresento o modo como a textualidade infográfica eletrônica produz a estereotipia e a contenção de sentidos ao funcionar sob o efeito de pré-construídos, bem como são produzidos efeitos de velocidade para a leitura. efeito de pré-construídos, bem como são produzidos efeitos de velocidade para a leitura.

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Abu Arqub, Omar. "Reproducing Kernel Algorithm for the Analytical-Numerical Solutions of Nonlinear Systems of Singular Periodic Boundary Value Problems." Mathematical Problems in Engineering 2015 (2015): 1–13. http://dx.doi.org/10.1155/2015/518406.

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The reproducing kernel algorithm is described in order to obtain the efficient analytical-numerical solutions to nonlinear systems of two point, second-order periodic boundary value problems with finitely many singularities. The analytical-numerical solutions are obtained in the form of an infinite convergent series for appropriate periodic boundary conditions in the spaceW230,1, whilst two smooth reproducing kernel functions are used throughout the evolution of the algorithm to obtain the required nodal values. An efficient computational algorithm is provided to guarantee the procedure and to confirm the performance of the proposed approach. The main characteristic feature of the utilized algorithm is that the global approximation can be established on the whole solution domain, in contrast with other numerical methods like onestep and multistep methods, and the convergence is uniform. Two numerical experiments are carried out to verify the mathematical results, whereas the theoretical statements for the solutions are supported by the results of numerical experiments. Our results reveal that the present algorithm is a very effective and straightforward way of formulating the analytical-numerical solutions for such nonlinear periodic singular systems.

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Thi Dung, Luu, Doan Huu Thien, Nguyen Thi Ly, Nguyen Thi Hong Dinh, Be Thi Tham, Nguyen Hoang Tung, and Pham Van Hung. "RT-PCR test for specific indentification of influenzavirus (A/H5N1) in vaccine." JOURNAL OF CONTROL VACCINES AND BIOLOGICALS, no. 1 (December 31, 2021): 66–77. http://dx.doi.org/10.56086/jcvb.vi1.6.

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RT-PCR (Reverse transcription - Polymerase Chain Reaction) is applied to determine the presence of influenza virus A/ H5N1 in vaccine, and to develop an identity process for specific virus strain A/H5N1 in influenza vaccine A/H5N1. Selected samples included: Ivacflu-A/H5N1 vaccine (Institute of Vaccines and Biologicals), Vaxigrip vaccine (Sanofi Pasteur), Influenza virus strain A/ Vietnam/1194/2004(A/H5N1) (NIBSC) was used as positive control; vaccine Varivax (MSD) and DNA/RNA free water was used as negative controls. The results showed that virus strain A/H5N1 was identified as production of RT-PCR that were positive with amplified primer pairs of 2 specific gene sequences of HA whose length 428 and 249 bp. Before starting RT-PCR, it was necessary to eliminate aluminum and the components of RT-PCR reaction included: 5X QIAGEN OneStep RT-PCR Buffer(5µl); dNTP (1µl); forward and reverse primers (1,5 µl); Enzyme (1 µl), H2 O (10 µl), ARN template (5 µl) and thermal cycle of RT- PCR reaction was: 50oC (30 minutes); 95OC (15 minutes); 94OC (30 seconds); 55OC (30 seconds); 72OC (1 minute); 72OC (10 minutes), 45 cycles.

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Gambino, Giorgio, and Ivana Gribaudo. "Simultaneous Detection of Nine Grapevine Viruses by Multiplex Reverse Transcription-Polymerase Chain Reaction with Coamplification of a Plant RNA as Internal Control." Phytopathology® 96, no. 11 (November 2006): 1223–29. http://dx.doi.org/10.1094/phyto-96-1223.

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A multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for simultaneous detection of nine grapevine viruses: Arabis mosaic virus, Grapevine fanleaf virus, Grapevine virus A, Grapevine virus B, Rupestris stem pitting-associated virus, Grapevine fleck virus, Grapevine leafroll-associated virus-1, -2, and -3, in combination with a plant RNA internal control used as an indicator of the effectiveness of RNA extraction and RT-PCR. Primers were designed from conserved regions of each virus and their specificity was confirmed by sequencing PCR products. Two plant total RNA extraction methods (silica capture and modified RNeasy method) and two RT-PCR systems (onestep and two-step) were evaluated to develop a reliable protocol for mRT-PCR. One to nine fragments specific for the viruses were simultaneously amplified from infected samples and identified by their specific molecular sizes in agarose gel electrophoresis. In the two-step mRT-PCR, the detection limits were 10-3 or 10-4 extract dilutions, depending on the virus. Leaves, phloem from dormant cuttings, and in vitro plantlets from 103 naturally infected and healthy grapevines were analyzed. The mRT-PCR provided a reliable and rapid method for detecting grapevine viruses from a large number of samples.

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Banerjee, S. M., N. R. Williams, T. I. Davidson, S. El Sheikh, M. Tran-Dang, S. Davison, D. Ghosh, and M. R. S. Keshtgar. "The use of onestep nucleic acid amplification (OSNA) and tumour related factors in the treatment of axillary breast cancer: A predictive model." European Journal of Surgical Oncology (EJSO) 42, no. 5 (May 2016): 641–49. http://dx.doi.org/10.1016/j.ejso.2016.02.245.

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Faraezi, Sharifa, Md Sharif Khan, Ferzana Zaman Monira, Abdullah Al Mamun, Tania Akter, Mohammad Al Mamun, Mohammad Mahbub Rabbani, Jamal Uddin, and A. J. Saleh Ahammad. "Sensitivity Control of Hydroquinone and Catechol at Poly(Brilliant Cresyl Blue)-Modified GCE by Varying Activation Conditions of the GCE: An Experimental and Computational Study." ChemEngineering 6, no. 2 (March 28, 2022): 27. http://dx.doi.org/10.3390/chemengineering6020027.

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The poly(brilliant cresyl blue) (PBCB)-modified activated glassy carbon electrode (AGCE) shows the catalytic activity toward the oxidation of hydroquinone (HQ) and catechol (CT). The modified electrode can also separate the oxidation peaks of HQ and CT in their mixture, which is not possible with bare GCE. These properties of the modified electrode can be utilized to fabricate an electrochemical sensor for sensitive and simultaneous detection of HQ and CT. In this study, an attempt is made to control the sensitivity of the modified electrodes. This can be accomplished by simply changing the activation condition of the GCE during electropolymerization. GCE can be activated via one-step (applying only oxidation potential) and two-step (applying both oxidation and reduction potential) processes. When we change the activation condition from onestep to twosteps, a clear enhancement inpeak currents of HQ and CT is observed. This helps us to fabricate a highly sensitive electrochemical sensor for the simultaneous detection of HQ and CT. The molecular dynamics (MD) simulation is carried out to explain the experimental data. The MD simulations provide the insight adsorption phenomena to clarify the reasons for higher signals of CT over HQ due to having meta-position –OH group in its structure.

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Abbai, N. S., P. Moodley, T. Reddy, T. G. Zondi, S. Rambaran, K. Naidoo, and G. Ramjee. "Clinical Evaluation of the OneStep Gonorrhea RapiCard InstaTest for Detection of Neisseria gonorrhoeae in Symptomatic Patients from KwaZulu-Natal, South Africa: TABLE 1." Journal of Clinical Microbiology 53, no. 4 (January 21, 2015): 1348–50. http://dx.doi.org/10.1128/jcm.03603-14.

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We evaluated a point-of-care test for the detection ofNeisseria gonorrhoeaein patients attending a public health clinic in KwaZulu-Natal, South Africa. The test showed a low sensitivity against PCR and culture (<40%); however, a higher specificity was observed (>95%). This test is unsuitable as a screening tool for gonorrhea.

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Stephen, MSc, Stephen, Chiwoneso Gwyneth Elizabeth Muchaneta-Kubara, PhD, Marshall Wesley Munjoma, PhD, and Gibson Mandozana, PhD. "Evaluation of Cortez Onestep Chlamydia RapicardTM Insta Test for the Detection of Chlamydia Trachomatis in Pregnant Women at Mbare Polyclinic in Harare, Zimbabwe." International Journal of MCH and AIDS (IJMA) 6, no. 1 (December 31, 2016): 19. http://dx.doi.org/10.21106/ijma.150.

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Background: Cervical chlamydia infection poses high risk of pregnancy complications and neonatal infection. Reference methods for the detection of chlamydia infection are not available for routine use in developing countries. Point-of-care (POC) tests can bridge this gap. This study evaluated Cortez Onestep Chlamydia RapicardTM insta test for the detection of Chlamydia trachomatis in pregnant women at Mbare Polyclinic and determined the prevalence of C. trachomatis.Methods: This was a cross sectional study in 242 pregnant women aged ≥18 years attending their first ANC visit at Mbare polyclinic in Harare, Zimbabwe. Data collection form was used to obtain demographic and predisposing factors to Chlamydia infection and two endocervical swabs were collected from each patient. One specimen was examined by the POC test at the clinic and the other by SDA method in the laboratory.Results: The sensitivity, specificity, positive and negative predictive values of the rapid kit were 71.4%, 99.6%, 90.9% and 98.3% respectively. Prevalence of C. trachomitis was 5.8% by SDA method.Conclusion and Global Health Implications: The kit’s sensitivity (71.4%) and specificity (99.6%) implies that the rapid test is an important test which needs further evaluations. The prevalence of C. trichomitis of 5.8% is comparable to studies done elsewhere in Africa.Key words: Chlamydia trachomatis • Antenatal Clinic • Point of Care Tests • Rapid Test • Cortez One Step Chlamydia TestCopyright © 2017 Stephen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Hayashi, Mika, Yoshiki Yamashita, Atsushi Hayashi, Yoko Yoshida, Sachiko Kawabe, Masami Hayashi, Yoshito Terai, Hideki Kamegai, and Masahide Ohmichi. "Expression and Epigenetic Change of the AR and FSHR Genes in the Granulosa Cells of Endometriosis Patients." Genetics & Epigenetics 4 (January 2012): GEG.S9877. http://dx.doi.org/10.4137/geg.s9877.

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Background Endometriosis is one of the most common gynecological diseases associated with infertility. Endometriosis may affect the androgen receptor (AR) mRNA expression in human granulosa cells and the methylation of the promoter region of AR. We investigated 28 patients with endometriosis and 47 subjects without endometriosis undertaking IVF treatment. Methods Granulosa cells were obtained from 28 patients with endometriosis and 47 subjects without endometriosis as a control. Expressions of AR and FSHR mRNA were then evaluated by OneStep real-time PCR analysis, and the level of methylation of the promoter region was qualified by methylation-specified PCR (MSP). Results The expression of AR mRNA in the endometriosis group was statistically lower than that in the control group. As well, FSHR mRNA expression in the control group showed a positive correlation with AR mRNA expression; however, there was no such correlation in endometriosis patients. In the control group, AR mRNA expression was statistically higher in pregnant subjects compared with non-pregnant subjects; however, in the endometriosis group, no significant difference was identified. The promoter of AR was heavily methylated in all endometriosis cases; however, only 5 (45.4%) were methylated in the control group. Conclusion Lower AR mRNA expression and methylation of the AR promoter region might affect the expression of AR and FSHR the presence of endometriosis, thus leading to a disturbance in the regulation of AR and FSHR.

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Brindle, Frances A. "Use of Native Vegetation and Biostimulants for Controlling Soil Erosion on Steep Terrain." Transportation Research Record: Journal of the Transportation Research Board 1819, no. 1 (January 2003): 203–9. http://dx.doi.org/10.3141/1819a-30.

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Native grass species are increasingly requested for use on revegetation and restoration projects following land disturbance. Native species can be slow to establish, a problem when the goals are to obtain quick ground cover to protect against rainfall impact and sheet and rill erosion and to promote root growth to stabilize soil on steep terrain. In addition, disturbed land is subject to invasion by weed species, creating competition with desirable plants for soil nutrients and moisture. Organic soil amendments and soil stabilizers were used on a large restoration project in northwest Oregon to provide soil conditions that would foster a healthy stand of native grasses and forbes (broadleaf plants) while holding the soil in place until plant establishment could occur. Organic soil amendments were prescribed that would improve soil structure, aid nutrient availability, and provide humic acids and bacterial activators. Organic mulch was added to provide the nutrient energy source for soil microorganisms. The amendments were mixed in a slurry and applied in a onestep hydroseeding application during the late fall of 2001. The materials were applied along with the native seed mixture with a mechanically agitated hydroseeding machine and sprayed on the surface in a pressurized spray. The results of the field evaluation were favorable for the ability of the soil to resist water erosion through the initial rainy season and the native species establishment after application.

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Kesler, Olivera. "Plasma Spray Processing of Solid Oxide Fuel Cells." Materials Science Forum 539-543 (March 2007): 1385–90. http://dx.doi.org/10.4028/www.scientific.net/msf.539-543.1385.

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Plasma spray processing is a low-cost, rapid manufacturing technique that is widely used industrially for fabrication of thermal barrier and wear- and corrosion-resistant coatings. Because the technique can be used to rapidly deposit coatings of high melting temperature materials with good substrate adhesion, it has also been applied to the production of individual component layers in tubular solid oxide fuel cells (SOFCs), and more recently, in planar SOFCs. The use of plasma spray processing for the fabrication of fuel cell components presents unique challenges, due to the high porosities required for the electrode layers and fully dense coatings required for electrolytes. Application of plasma spray processing for the manufacture of solid oxide fuel cells is discussed, with consideration of potential advantages of the technique compared to standard SOFC wet ceramic processing routes. Major challenges faced in the adaptation of the processing method to solid oxide fuel cell manufacture are discussed, along with current research approaches being used to overcome these challenges. Recent developments in the use of the technique for the rapid onestep manufacturing of direct oxidation SOFC anodes are discussed, for composite material combinations that cannot be co-sintered due to widely divergent melting points. The impacts of plasma sprayed coating properties on solid oxide fuel cell performance are considered, and implications of the use of the technique on overall stack and system manufacturing costs are discussed.

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Alfawaz, Yasser. "Impact of Polishing Systems on the Surface Roughness and Microhardness of Nanocomposites." Journal of Contemporary Dental Practice 18, no. 8 (2017): 647–51. http://dx.doi.org/10.5005/jp-journals-10024-2100.

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ABSTRACT Aim The aim of this article is to evaluate the influence of finishing and polishing techniques on the surface roughness and microhardness of two composite resins with two different types of polishing systems. Materials and methods A total of 30 disk-shaped specimens of nanocomposite Filtek™ Z350 and Ceram-X® were prepared. They were divided into three groups. The control group (n = 10) received no finishing and polishing. The remaining specimens were divided into two groups, and they received polishing and finishing either with PoGo or Sof-Lex system. After the polishing procedures, average surface roughness (Ra) was assessed with a surface profilometer. The microhardness was determined using a Vickers hardness test. The data were tabulated and analyzed. Results The smoothest surfaces were noticed with the control group (Mylar strips) in both composite materials tested. The PoGo one-step polishing system showed significantly better surface roughness compared with the Sof-Lex polishing system. The microhardness did not show any significant variations after finishing and polishing. Conclusion It can be concluded that the use of PoGo® onestep polishing system resulted in smoother surface with both composite materials studied compared with the Sof-Lex system. The finishing and polishing system had little influence on the surface microhardness. Clinical significance The finishing procedure and polishing system can affect the physical properties and performance of resin composites. How to cite this article Alfawaz Y. Impact of Polishing Systems on the Surface Roughness and Microhardness of Nanocomposites. J Contemp Dent Pract 2017;18(8):647-651.

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Tarnowski, C. G., P. A. Worlock, S. Ulanovsky, and S. Gómez Talquenca. "First Report of Rupestris stem pitting associated virus in Argentina." Plant Disease 86, no. 8 (August 2002): 921. http://dx.doi.org/10.1094/pdis.2002.86.8.921b.

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Rupestris stem pitting associated virus (RSPaV), a component of the rugose wood complex (RWC), is a worldwide graft transmissible disease of grapevines (Vitis vinifera L.). RSPaV has a single-stranded 8,726-nt RNA genome, belongs to the genus Foveavirus, and is often associated with Rupestris stem pitting (RSP) disease (2). In 1995, a grapevine sanitary selection program was implemented in Mendoza to investigate this and other grapevine viral diseases. RSP can be diagnosed when V. rupestris cv. St. George is used as a woody indicator for biological indexing. Chip-bud inoculated St. George plants developed a row of small pits and grooves on the wood cylinder below the graft or around and below the inoculated point (1,2). After three seasons in the field, 15 accessions with RSP wood markings were observed. Mature leaves and bark shavings were extracted, partially purified, and analyzed by a onestep reverse transcription polymerase chain reaction method. The expected 339-bp band was found in only six of the positively indexed samples using the specific 13/14 primer pair (2). Other viruses associated with RWC have been detected in Argentina, but to our knowledge, this is the first report of RSPaV. References: (1) A. C. Goheen. Page 53 in: Compendium of Grape Diseases, R. C. Pearson and A. C. Goheen, eds. American Phytopatological Society, St. Paul, MN, 1988. (2) B. Meng. Rupestris stem pitting: Insights on etiology and development of reverse transcription-polymerase chain reaction and immunoassays for diagnosis. Ph.D. Diss. Cornell University, Ithaca, NY, 1999.

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Rahman, Md Ferdous, Sheikh Rashel Al Ahmed, Golam Saklayen, and Abu Bakar Md Ismail. "Experimental Study on Silicon Nanocrystals Rich Lanthanum Fluoride Films for Future Electronic Devices." Rajshahi University Journal of Science and Engineering 44 (November 19, 2016): 61–66. http://dx.doi.org/10.3329/rujse.v44i0.30388.

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Feasibility for the future electronic devices a thorough investigation on Silicon nanocrystals (Si-NCs) rich Lanthanum Fluoride (LaF3) film fabricated using a novel onestep chemical method has been reported here. Colloidal solution of Si-NCs in hydrofluoric acid (HF) was prepared from meso-porous silicon by ultrasonic vibration (sonication). On a silicon (Si) substrate LaCl3 solution in HCL is allowed to react with the colloidal solution of prepared Si-NCs. LaCl3 reacts with HF of Si-NCs solution and produces LaF3 crystals that deposits on the silicon substrate as a film embedding Si-NCs. This is a novel single step chemical way of depositing LaF3 insulating layer embedding Si-NCs (LaF3:Si-NCs). The XRD and EDX analysis of the deposited film show a polycrystalline and non-stoichiometric nature of LaF3. The presence of Si-NCs was confirmed by SEM. Application of this material has been tested for low-voltage operating non-volatile memory (NVM) and Schottky junction solar cells. The Al/LaF3:Si-NCs/Al structure as NVM offered a memory window of 525 mV at a programming and erasing bias of 2V. LaF3:Si-NCs films showed strong light absorption. Current-Voltage (I-V) characteristics of the Schottky device in ITO/LaF3:Si-NCs/Al structure showed a dependency on the incident light intensity where current was varied in the range of 5 mA to 40 mA and under various light illumination i.e., 400 lux to 1200 lux. Experimental results show a lot of promise of Si-NCs-rich LaF3 film to be used as an insulating film in non-volatile memory as well as a photoactive material in Schottkey junction solar cell.

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Tosti, Silvano, Alfonso Pozio, Luca Farina, and Alessia Santucci. "Hydrogen and Oxygen Production via Water Splitting in a Solar-Powered Membrane Reactor—A Conceptual Study." Hydrogen 2, no. 1 (January 7, 2021): 18–32. http://dx.doi.org/10.3390/hydrogen2010002.

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Among the processes for producing hydrogen and oxygen from water via the use of solar energy, water splitting has the advantage of being carried out in onestep. According to thermodynamics, this process exhibits conversions of practical interest at very high temperatures and needs efficient separation systems in order to separate the reaction products, hydrogen and oxygen. In this conceptual work, the behavior of a membrane reactor that uses two membranes perm-selective to hydrogen and oxygen is investigated in the temperature range 2000–2500 °C of interest for coupling this device with solar receivers. The effect of the reaction pressure has been evaluated at 0.5 and 1 bar while the permeate pressure has been fixed at 100 Pa. As a first result, the use of the membrane perm-selective to oxygen in addition to the hydrogen one has improved significantly the reaction conversion that, for instance, at 0.5 bar and 2000 °C, moves from 9.8% up to 18.8%. Based on these critical data, a preliminary design of a membrane reactor consisting of a Ta tubular membrane separating the hydrogen and a hafnia camera separating the oxygen is presented: optimaloperating temperature of the reactor results in being around 2500 °C, a value making impracticable its coupling with solar receivers even in view of an optimistic development of this technology. The study has verified that at 2000 °C with a water feed flow rate of 1000 kg h−1 about 200 and 100 m3 h−1 of hydrogen and oxygen are produced. In this case, a surface of the hafnia membrane of the order of hundreds m2 is required: the design of such a membrane device may be feasible when considering special reactor configurations.

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ASSURIAN, ANGELA, HELEN MURPHY, ALICIA SHIPLEY, HEDIYE NESE CINAR, ALEXANDRE DA SILVA, and SONIA ALMERIA. "Assessment of Commercial DNA Cleanup Kits for Elimination of Real-Time PCR Inhibitors in the Detection of Cyclospora cayetanensis in Cilantro." Journal of Food Protection 83, no. 11 (June 9, 2020): 1863–70. http://dx.doi.org/10.4315/jfp-20-139.

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ABSTRACT Inhibited reactions have occasionally been observed when cilantro samples were processed for the detection of Cyclospora cayetanensis using quantitative real-time PCR (qPCR). Partial or total inhibition of PCR reactions, including qPCR, can occur, leading to decreased sensitivity or false-negative results. If inhibition occurs, this implies the need for additional purification or cleanup treatments of the extracted DNA to remove inhibitors prior to molecular detection. Our objective was to evaluate the performance of five commercial DNA cleanup kits (QIAquick purification kit from Qiagen [kit 1], OneStep PCR inhibitor removal by Zymo Research [kit 2], NucleoSpin genomic DNA cleanup XS from Macherey-Nagel [kit 3], DNA IQ system by Promega [kit 4], and DNeasy PowerPlant pro kit from Qiagen [5]) to minimize qPCR inhibition using the U.S. Food and Drug Administration–validated Bacteriological Analytical Manual (BAM) Chapter 19b method for detection of C. cayetanensis in cilantro samples containing soil. Each of the five commercial DNA cleanup kits evaluated was able to reduce the qPCR internal amplification control cycle threshold values to those considered to be normal for noninhibited samples, allowing unambiguous interpretation of results in cilantro samples seeded at both a high oocyst level (200 oocysts) and a low oocyst level (10 oocysts). Of the five kits compared, kits 1, 2, and 3 did not show significant differences in the detection of C. cayetanensis, while significantly higher cycle threshold values, indicating lower recovery of the target DNA, were observed from kits 4 and/or 5 in samples seeded with 200 and 10 oocysts (P < 0.05). This comparative study provides recommendations on the use of commercial cleanup kits which could be implemented when inhibition is observed in the detection of C. cayetanensis in cilantro samples using the BAM Chapter 19b method. HIGHLIGHTS

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Bhandary, Shreetha, M. Surya Chaitanya Reddy, MC Mahesh, J. Pramod, and Ashwija Shetty. "Evaluation of Effect of Different Cavity Disinfectants on Shear Bond Strength of Composite Resin to Dentin using Two-Step Self-Etch and One-Step Self-Etch Bonding Systems: A Comparative in vitro Study." Journal of Contemporary Dental Practice 14, no. 2 (2013): 275–80. http://dx.doi.org/10.5005/jp-journals-10024-1313.

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ABSTRACT Aim and objectives To evaluate the effect of 2% chlorhexidine, 2% sodium hypochlorite, 3% hydrogen peroxide on shear bond strength of composite resin to dentin using two-step and onestep self-etch bonding systems and to study the mode of failure of specimens under stereomicroscope. Materials and methods Eighty extracted sound human posterior teeth were used. The occlusal surfaces were ground to expose the dentin and were then randomly divided into 4 main groups. In group I, no cavity disinfectant was used and served as control. In groups II, III, IV, 2% chlorhexidine, 2% sodium hypochlorite and 3% hydrogen peroxide were used as cavity disinfectants respectively. Each group was then divided into two subgroups of 10 teeth each according to the bonding agent used, two-step self-etch (Adper SE Plus) and one-step self-etch (Adper Easy One) respectively. A transparent cylindrical plastic tube was loaded with microhybrid composite and placed over the dentin and light cured for 40 seconds. The specimens were subjected to shear stress in the universal testing machine. Results Pretreatment with 2% chlorhexidine, 2% sodium hypochlorite and 3% hydrogen peroxide, had a negative effect on the shear bond strength of self-etching bonding systems. Conclusion The highest bond strength was found in 2% chlorhexidine group followed by 2% sodium hypochlorite group and the lowest bond strength was found in 3% hydrogen peroxide group. Clinical significance All three cavity disinfectants used in this study reduced the shear bond strength and hence should be used with caution. How to cite this article Reddy MSC, Mahesh MC, Bhandary S, Pramod J, Shetty A. Evaluation of Effect of Different Cavity Disinfectants on Shear Bond Strength of Composite Resin to Dentin using Two-Step Self-Etch and One-Step Self-Etch Bonding Systems: A Comparative in vitro Study. J Contemp Dent Pract 2013;14(2):275-280.

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Parrott, Gretchen, Takeshi Kinjo, Daijiro Nabeya, Ayako Uehara, Saifun Nahar, Kazuya Miyagi, Shusaku Haranaga, Masao Tateyama, and Jiro Fujita. "Evaluation of Anyplex™ II RV16 and RB5 real-time RT-PCR compared to Seeplex ® RV15 OneStep ACE and PneumoBacter ACE for the simultaneous detection of upper respiratory pathogens." Journal of Infection and Chemotherapy 23, no. 12 (December 2017): 859–61. http://dx.doi.org/10.1016/j.jiac.2017.07.013.

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Le Corre, Nicole, Regina Pérez, Cecilia Vizcaya, Constanza Martínez-Valdebenito, Tania López, Marcela Monge, Romina Alarcón, et al. "Relevancia de la co-detección de virus respiratorios en la severidad de la infección respiratoria aguda en niños hospitalizados." Andes Pediatrica 92, no. 3 (June 22, 2021): 349. http://dx.doi.org/10.32641/andespediatr.v92i3.1756.

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La reacción de polimerasa en cadena (RPC) múltiple permite detectar simultáneamente virus respiratorios, planteando interrogantes sobre la relevancia de cada uno de ellos en el cuadro clínico.Objetivo: Evaluar la contribución de factores clínicos, epidemiológicos y virológicos para interpretar el cuadro clínico de niños hospitalizados por infección respiratoria aguda (IRA) con co-detección viral.Pacientes y Método: Se incluyeron los pacientes pediátricos ≤ 15 años, hospitalizados por IRA en el Hospital Clínico de la Red de Salud UC-CHRISTUS entre los meses de junio y octubre de 2014, y que presentaron un panel molecular respiratorio positivo. Las muestras respiratorias (hisopado nasofaríngeo, aspirado traqueal o lavado broncoalveolar) con paneles positivos por técnica Seeplex® RV15 OneStep ACE Detection Seegene®, fueron analizadas con una segunda técnica (xTAG-RVP-FASTv2 Luminex®, USA), la cual permite la detección simultánea y semi-cuantitativa de 17 virus respiratorios. Se recolectaron antecedentes clínicos y epidemiológicos.Resultados: Se identificó un virus en 42/57 niños (74%) y más de uno en 15/57 (26%). La hospitalización en cuidados intensivos (UCI) fue más frecuente en pacientes con co-detección (OR = 5,5; IC 95%: 1,5-19,6). Los virus más detectados fueron rinovirus/enterovirus (HRV/EV) (29%) y virus respiratorio sincicial (VRS) (25%) y la co-detección más habitual fue HRV/EV-VRS (33%). La infección HRV/EV presentó más frecuentemente imágenes intersticiales mientras que VRS condensaciones (p = 0,002). Para HRV/ EV, las medianas de intensidad de fluorescencia (MFI, semi-cuantificación) fueron de 1788 y 2456, en co-detección y agente único, respectivamente (p = 0,022). En niños con co-detección HRV/EV, se observó una estadía hospitalaria más prolongada comparado con su detección aislada (5 versus 3 días, p = 0,028).Conclusión: La co-detección viral es frecuente en niños hospitalizados con IRA y se asocia a mayor hospitalización en UCI. Destaca la presencia de HRV/EV en la co-detección viral y una estadía hospitalaria más prolongada. Más estudios son necesarios para definir la relevancia de los virus identificados en co-detección en pacientes pediátricos hospitalizados.

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Khoo, Larry T., Nan Fu Chen, Sean Armin, Eric Stiner, Juan Dipp, Ricardo Flores, and Sylvain Palmer. "A novel minimally invasive percutaneous facet augmentation device for the treatment of lumbar radiculopathy and axial back pain: technical description, surgical technique and case presentations." Coluna/Columna 8, no. 1 (March 2009): 84–91. http://dx.doi.org/10.1590/s1808-18512009000100016.

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OBJECTIVE: to describe a new posterior minimally invasive method of facet stabilization for treatment of the degenerating lumbar motion segment. The biomechanics of this Percudyn (Interventional Spine; Irvine, CA) system are distinct from that of other interspinous dynamic stabilization systems as it acts bilaterally directly within the middle column of the spine. Based on biomechanical evalution, the paired prosthesis supports, cushions, and reinforces the facet complexes by limiting both extension and lateral bending thereby maintaining central and foraminal volumes. METHODS: the Percudyn device consists of a pedicle anchor upon which sits a cushioning polycarbonate-urethane stabilizer that serves as a mechanically reinforcing stop between the inferior and superior articular facets. A 1.5 cm skin incision is made bilaterally over the lower pedicle of the treated segment through which a Jamshidi needle is percutaneously targeted under biplanar fluoroscopic guidance into the caudal aspect of the superior articular process directly underneath the lip of the inferior facet from the level above. Progressive onestep tubular dilation is then performed to secure a small disposable working portal. Through this access, the Percudyn stabilizers are then placed over the wire and anchored bilaterally into the inferior pedicles of the degenerated motion segment. RESULTS: three patients (ages 26-41, male) with significant low back pain as well as radiculopathy with lateral recess stenosis from a large disc herniation/ ligamentum and facet hypertrophy (L4-5 and/or L5-S1) underwent a minimally invasive decompression/ discectomy and bilateral Percudyn placement at each disease level. Each patient had significant relief of both his radiculopathy and axial back pain post-operatively and was discharged home within 18 hours without sequelae. CONCLUSION: this novel technique of percutaneous posterior facet augmentation allows for safe placement of bilateral middle column prostheses that act as mechanical cushions between the articulating facets thereby limiting extension and lateral bending and also preventing compression of the neural elements. As the Percudyn device serves to reinforce the middle column directly at the level of the facet, it represents a new class of posterior motionpreserving stabilization which may serve to mitigate segmental axial back pain as has been described for other posterior dynamic stabilization systems.

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Salah, Samer, Walid Naser, Omar Jaber, yacob saleh, Rawan Mohammed Mahmoud Mustafa, Ramiz Abuhijlih, Fawzi Abuhijla, et al. "The MGMT (O6-methylguanine–DNA methyltransferase) promotes methylation and clinical outcomes of salvage temozolomide and irinotecan chemotherapy in progressive Ewing sarcoma: A preliminary report." Journal of Clinical Oncology 39, no. 15_suppl (May 20, 2021): e23507-e23507. http://dx.doi.org/10.1200/jco.2021.39.15_suppl.e23507.

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e23507 Background: There remains an unmet need to identify prognostic and predictive molecular biomarkers in advance Ewing sarcoma (ES). We sought to assess the influence of MGMT promoter methylation status on response rate, time to progression (TTP), and overall survival (OS) following salvage irinotecan and temozolomide (IT) chemotherapy. Methods: Data of advanced ES patients, treated with IT chemotherapy from Jan, 2014 to Jan, 2020 were retrospectively collected. Patients were required to have previously received and progressed after vincristine, doxorubicin, and cyclophosphamide alternating with ifosfamide and etoposide (VDC-IE). MGMT promoter methylation status was assessed by Methylation Sensitive Restriction Enzyme quantitative-PCR (MSRE-qPCR) on non-decalcified Formalin-fixed paraffin embedded (FFPE) tissue using internally developed primers and the OneStep qMethyl Kit (ZYMO RESEARCH CORP). Responses were assessed by response evaluation criteria in solid tumors (RECIST v. 1.1). TTP and OS were assessed by the Kaplan-Meier method. Survival comparisons were performed by the Log-rank test. Results: Herein, we present data of the preliminary analysis of the first 18 patients who underwent MGMT promoter methylation testing. Patients had a median age of 18 years (range: 5-34 years), and were predominantly male ( n=11; 61%). The primary tumor was located in the pelvis in 9 patients (50%), femur in 3 (17%), tibia in 2 (11%), kidney in 2 (11%), chest wall in one (6%), and scapula in one patient (6%). IT was given in a second ( n=15) or third-line setting ( n=3). At the time of initiation of IT, 13 patients (72%) had distant metastasis, and 5 (28%) had unresectable local progression in the pelvis ( n=4) or chest wall ( n=1). The mean percentage of MGMT promoter methylation was 29% (range: 3-98%). Five patients (28%) had methylated MGMT promoter, whereas the remaining had partially methylated ( n=6; 33%) or unmethylated ( n=7;39%) promoter. Five patients (28%) had objective response, with no observed difference according to MGMT promoter methylation ( p=0.58 for comparison between methylated and unmethylated/ partially methylated). Median TTP was 4.9 and 2.2 months for patients with methylated and partially methylated/ unmethylated MGMT respectively; p=0.76. The corresponding median OS was 69.4 and 14.3 months in favor of the methylated group; p=0.3. Conclusions: This preliminary data suggests a possible prognostic role for MGMT promoter methylation, with a markedly extended median OS for the methylated group. Nevertheless, the median OS difference did not reach statistical significance in this preliminary analysis. We plan to report data of the final analysis after finalizing MGMT testing for the rest of our study patients.

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Chandi, Dhruba Hari. "Prevalence of dengue virus infection among population of Bhaili visiting tertiary health institution; Chhattisgarh." Asian Journal of Medical Sciences 11, no. 2 (February 29, 2020): 30–34. http://dx.doi.org/10.3126/ajms.v11i2.27345.

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Background: Dengue fever, dengue haemorrhagic fever and dengue shock syndrome of infection is distinct clinical forms which is caused by Dengue Virus that belong to a member of the Flaviviridae family. It is most important arthropod borne viral disease that causes morbidity and mortality. Dengue virus infection is a major public health problem growing in worldwide and it is estimated about 2.5 billion people of world are at risk of this infection. In India several parts of the country it is an endemic disease. Aims and Objective: The current work is an attempt to review current perspectives of dengue infection among population of Bhilai visiting tertiary health institution. In study also we have compared the serological profiles of the dengue cases. Material and Methods: Samples received were performing retrospective analysis in the department of microbiology and process in the departmental microbiology laboratory obtaining during monsoon season. The duration of fever (in days) and other relevant clinical information were recorded from the requisition form. Rapid test for dengue as NS1Ag, IgG and IgM test were performed in accordance with the manufacturer’s instructions. The dengue NS1Ag, IgG and IgM rapid test is an in vitro immunochromatographic test (ICT) which is a onestep assay designed for the qualitative determination of dengue NS1Ag, IgG and IgM in human serum for the diagnosis dengue infection. Result: During the study period total 1308 serum samples were collected from suspected Dengue fever patients. Among 1308 samples 412(31.5%) samples were found to be positive dengue fever. In the month of August maximum number of sample were received. Out of 1308 patients with dengue suspected patients 792(60.6%) were male and 516(39.4%) were female. Among 60.6% of male 28.2% were positive and out of 39.4% of female 36.6% were positive for dengue. Age between 10-20 years old (55.83%) patients were most affected age group and 70-80 years old (8.77%) age group were least affected. Among the dengue positive patients, dengue parameter associated with thrombocytopenia was also recorded which showed that there was no significant difference between the parameters in relation to thrombocytopenia. Conclusion: Especially in developing countries like India where there are poor resources for diagnosis of dengue infection, the sensitivity of these tests is more than immunochromatographic Test (ICT). By NS1 assay early detection of dengue infection can help in early confirmation and management of this before its gets complicate. Therefore commercial available dengue NS1 antigen test kits provided additional laboratory diagnostic tool for early detection of dengue.

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Bulajić, A., J. Jović, S. Krnjajić, M. Petrov, I. Djekić, and B. Krstić. "First Report of Iris yellow spot virus on Onion (Allium cepa) in Serbia." Plant Disease 92, no. 8 (August 2008): 1247. http://dx.doi.org/10.1094/pdis-92-8-1247a.

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Iris yellow spot virus (IYSV; genus Tospovirus, family Bunyaviridae) is established in several European countries (France, Italy, The Netherlands, Poland, Slovenia, Spain, and the UK) and its distribution in the EU region has increased since 2002 (3). In July 2007, symptoms resembling those of IYSV were observed in an onion (Allium cepa) seed crop in the Sirig locality in Serbia. Onion plants exhibited characteristic symptoms of chlorotic or necrotic spindle and diamond-shaped lesions on the leaves and scapes. Symptomatic plants were found throughout the field and disease incidence was estimated at 80%. Leaf and scape samples were tested for the presence of IYSV and two other tospoviruses, Tomato spotted wilt virus (TSWV) and Impatiens necrotic spot virus (INSV), using commercial double-antibody sandwich (DAS)-ELISA diagnostic kits (Loewe Biochemica, Sauerlach, Germany). All samples tested negative for TSWV and INSV. IYSV was detected serologically in 26 of 34 onion samples. To determine an experimental host range, samples of IYSV-infected onion plants were homogenized in chilled 0.05 M phosphate buffer pH 7 containing 1 mM Na-EDTA, 5 mM Na-DIECA, and 5 mM Na-thioglycolate (2), and host plants were inoculated with the sap. Mechanical transmission of the virus occurred rarely. All inoculated test plants were assayed by DAS-ELISA and only four species tested positive for IYSV, but not in all replications. Inoculated Chenopodium quinoa developed local chlorotic lesions, Nicotiana tabacum cvs. Samsun and Prilep showed mild mosaic, while infected N. benthamiana were symptomless. For further confirmation of IYSV, conventional reverse transcription (RT)-PCR was performed on extracts made from symptomatic onion leaf material and from the ELISA-positive symptomless leaves of N. benthamiana. Total RNAs were extracted with an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and RT-PCR was carried out with the OneStep RT PCR Kit (Qiagen) following the manufacturer's instructions. The primer pair, IYSV56U/IYSV917L, covering the entire nucleocapsid (NC) gene was used for both amplification and sequencing (1). A product of the correct predicted size (896 bp) was obtained from each of the plants assayed, and that derived from isolate 605-SRB was purified (QUIAqick PCR Purification Kit, Qiagen) and sequenced (GenBank Accession No. EU586203). BLAST analyses revealed 86 to 97% sequence identity with the NC gene from all other IYSV. The highest identity (97%) was with leek and onion isolates (GenBank Accession Nos. EF427447 and EF19888) from Spain. To our knowledge, this is the first report of IYSV infection of onion seed crop in Serbia. Thorough inspections and subsequent testing would be needed to establish the distribution and incidence of IYSV in Serbia. References: (1) I. Robène-Soustrade et al. Plant Pathol. 55:288, 2006. (2) P. Roggero et al. Plant Dis. 86:950, 2002. (3) C. Sansford and J. Woodhall. Pest Risk Analysis for Iris Yellow Spot Virus. Online publication. Central Science Laboratory, Sand Hutton, UK, 2007.

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50

Stanković, I., A. Bulajić, A. Vučurović, D. Ristić, J. Jović, and B. Krstić. "First Report of Tomato spotted wilt virus on Gerbera hybrida in Serbia." Plant Disease 95, no. 2 (February 2011): 226. http://dx.doi.org/10.1094/pdis-10-10-0704.

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In May 2009, approximately 30% of plants within a greenhouse-grown Gerbera hybrida crop in Vranjska Banja (Pčinj District) in Serbia displayed chlorotic oak-leaf patterns followed by necrosis and distortion of leaves. Symptoms on naturally infected gerbera plants and local necrotic spots on Petunia × hybrida mechanically inoculated with infected gerbera sap using chilled 0.05 M phosphate buffer (pH 7) containing 1 mM Na-EDTA, 5 mM Na-DIECA, and 5 mM Na-thioglycolate (4) suggested the presence of a Tospovirus. Symptomatic leaves were tested for the presence of Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV), and Chrysanthemum stem necrosis virus (CSNV) by commercial double-antibody sandwich (DAS)-ELISA diagnostic kits (Loewe Biochemica, Sauerlach, Germany). Commercial positive and negative controls and extract from healthy gerbera tissue were included in each ELISA. All 20 tested plants were negative for INSV and CSNV. TSWV was detected serologically in 18 of 20 gerbera samples. The presence of TSWV in ELISA-positive symptomatic gerbera plants was further confirmed by conventional reverse transcription (RT)-PCR. Total RNAs were extracted with an RNeasy Plant Mini Kit (Qiagen, Hilden, Germany) and RT-PCR was conducted with the OneStep RT-PCR Kit (Qiagen) using Serbian tobacco TSWV isolate (GQ279731) and RNA extract from healthy gerbera as positive and negative controls, respectively. Two different sets of TSWV-specific primers, L1 TSWVR/L2 TSWVF (2) and M962/M66 (3), for a 276-bp fragment of the RNA-dependent RNA polymerase (RdRp) gene and a 897-bp fragment of the NSm gene, respectively, were used for both amplification and sequencing. RT-PCR analyses of each tested plant detected the presence of amplification fragments of expected size. The amplified products corresponding to part of the RdRp and NSm genes derived from the isolate 158-Gerb were purified (QIAquick PCR Purification Kit, Qiagen) and sequenced in both directions (GenBank Accession Nos. HQ246452 and HQ246453, respectively). Sequence analysis of the partial RdRp gene, conducted using MEGA4 software, revealed 91.1 to 98% nt identity (95.1 to 98.8% amino acid [aa] identities) with corresponding sequences of TSWV L RNA deposited in GenBank. The highest identity was found with an isolate from globe artichoke (AM940436) in Greece, and isolates from tomato (GQ279732), impatiens (GQ132190), and tobacco isolates (GQ279731, FJ189392, and FJ189393) found within Serbia. Analysis of the NSm sequence of isolate 158-Gerb demonstrated nucleotide identities varying between 90.6 and 99.6% (80.9 and 99.6% aa identities) with those of previously reported TSWV isolates. The highest identity was with tobacco isolate GQ373174 from Serbia. Therefore, while gerbera is one of the principal ornamental hosts of TSWV in the EPPO region (1), to our knowledge, this is the first report infecting gerbera in Serbia, which may have a devastating influence on its production. References: (1) Anonymous. OEPP/EPPO Bull. 29:465, 1999. (2) R. A. Mumford et al. J. Virol. Methods 46:303, 1994. (3) W. P. Qiu et al. Virology 244:186, 1998. (4) P. Roggero et al. Plant Dis. 86:950, 2002.

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Journal articles on the topic 'Onesteel'

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