Relevant bibliographies by topics / OMP26

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Academic literature on the topic 'OMP26'

Author: Grafiati
Published: 4 June 2021
Last updated: 4 February 2022

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Journal articles on the topic "OMP26"

1

Kyd, Jennelle M., and Allan W. Cripps. "Potential of a Novel Protein, OMP26, from Nontypeable Haemophilus influenzae To Enhance Pulmonary Clearance in a Rat Model." Infection and Immunity 66, no. 5 (May 1, 1998): 2272–78. http://dx.doi.org/10.1128/iai.66.5.2272-2278.1998.

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ABSTRACT A major outer membrane protein band of approximately 25 to 27 kDa is commonly observed in strains of Haemophilus influenzae. This study has investigated the potential of a 26-kDa protein (OMP26) from nontypeable H. influenzae (NTHI) as a vaccine candidate. OMP26 was used to immunize rats via intestinal Peyer’s patches, followed by an intratracheal boost. Immunization was found to significantly enhance bacterial clearance following pulmonary challenge with both the homologous NTHI strain and a different NTHI strain. Significant levels of anti-OMP26 were found in the serum and bronchoalveolar lavage from immunized rats, and isotypes of immunoglobulin G (IgG) were also measured in serum. Analysis of IgG isotypes present in serum following OMP26-immunization suggest that predominantly a T-helper 1-type response was induced. The OMP26 protein was amino-terminally sequenced and found to have no homology with the P5 of H. influenzae type b P5 or the fimbrin protein of NTHI, both can migrate upon sodium dodecyl sulfate-polyacrylamide gel electrophoresis at similar molecular masses but OMP26 has 100% homology with a segment of the H. influenzae Rd genome. The results of this study suggest that OMP26 may be a suitable vaccine candidate against NTHI infection and warrants continued investigation and characterization.
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El-Adhami, Wafa, Jennelle M. Kyd, David A. Bastin, and Allan W. Cripps. "Characterization of the Gene Encoding a 26-Kilodalton Protein (OMP26) from Nontypeable Haemophilus influenzae and Immune Responses to the Recombinant Protein." Infection and Immunity 67, no. 4 (April 1, 1999): 1935–42. http://dx.doi.org/10.1128/iai.67.4.1935-1942.1999.

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ABSTRACT A 26-kDa protein (OMP26) isolated and purified from nontypeableHaemophilus influenzae (NTHI) strain 289 has been shown to enhance clearance of infection following pulmonary challenge with NTHI in rats. DNA sequence analysis revealed that it was 99% identical to a gene encoding a cell envelope protein of the H. influenzaeRd strain (TIGR accession no. HI0916). The deduced amino acid sequence revealed a hydrophilic polypeptide rich in basic amino acids. Restriction fragment length polymorphism analysis suggested that the OMP26 gene was relatively conserved among isolates of NTHI. Analysis of the deduced amino acid sequence of the OMP26 gene from 20 different isolates showed that similarity with NTHI-289 ranged from 96.5% (1 isolate) to 99.5% (14 isolates). Two recombinant forms of OMP26, a full length 28-kDa protein (equivalent to preprotein) and a 26-kDa protein lacking a 23-amino-acid leader peptide (equivalent to processed protein), were assessed in immunization studies for the ability to induce an immune response that would be as effective as the native protein in enhancing the clearance of NTHI following pulmonary challenge in rats. Immunization with the recombinant protein that included the leader peptide was more effective in enhancing pulmonary clearance, and it induced a better cell-mediated response and higher titers of systemic and mucosal antibody. This study has characterized a 26-kDa protein from NTHI that shows significant potential as a vaccine candidate.
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Kyd, Jennelle M., Allan W. Cripps, Laura A. Novotny, and Lauren O. Bakaletz. "Efficacy of the 26-Kilodalton Outer Membrane Protein and Two P5 Fimbrin-Derived Immunogens To Induce Clearance of Nontypeable Haemophilus influenzae from the Rat Middle Ear and Lungs as Well as from the Chinchilla Middle Ear and Nasopharynx." Infection and Immunity 71, no. 8 (August 2003): 4691–99. http://dx.doi.org/10.1128/iai.71.8.4691-4699.2003.

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ABSTRACT The rat middle ear and lung clearance model has been used to show that the nontypeable Haemophilus influenzae 26-kDa outer membrane protein OMP26 is highly efficacious as a mucosal immunogen, inducing significantly enhanced clearance in immunized rats upon direct challenge of these two anatomic sites. Similarly, the chinchilla model of middle ear and nasopharyngeal clearance has been used to show that two P5 fimbrin adhesin-derived immunogens, LB1 and lipoprotein D (LPD)-LB1(f)2,1,3, are highly efficacious as parenteral immunogens. Both induced significantly augmented clearance of nontypeable H. influenzae upon challenge of these sites. Here, these three nontypeable H. influenzae immunogens in addition to six bovine serum albumin and keyhole limpet hemocyanin conjugates of the synthetic peptide LB1(f) were assayed for relative efficacy in the reciprocal rodent model system. OMP26 was assayed in the chinchilla host by a parenteral immunization route, with clearance of the middle ear and nasopharynx used as outcome measures. Both LB1 and LPD-LB1(f)2,1,3 were assayed in the rat host with a mucosal immunization route and clearance of nontypeable H. influenzae from the lungs and middle ears as outcome measures. Both of the immunogens were found to induce a high-titered and specific immune responses in the heterologous host system. Moreover, each was found to be highly efficacious in the reciprocal host system, providing strong support for the continued development and inclusion of both OMP26 and P5 fimbrin-derived peptides as candidate vaccine antigens directed at otitis media caused by nontypeable H. influenzae.
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Manterola, Lorea, Caterina Guzmán-Verri, Esteban Chaves-Olarte, Elías Barquero-Calvo, María-Jesús de Miguel, Ignacio Moriyón, María-Jesús Grilló, Ignacio López-Goñi, and Edgardo Moreno. "BvrR/BvrS-Controlled Outer Membrane Proteins Omp3a and Omp3b Are Not Essential for Brucella abortus Virulence." Infection and Immunity 75, no. 10 (July 30, 2007): 4867–74. http://dx.doi.org/10.1128/iai.00439-07.

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ABSTRACT The Brucella abortus two-component regulatory system BvrR/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22). Disruption of bvrS or bvrR generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, and complement. Consequently, the role of Omp3a and Omp3b in virulence was examined. Similar to bvrS or bvrR mutants, omp3a and omp3b mutants displayed increased attachment to cells, indicating surface alterations. However, they showed unaltered permeability; normal expression of Omp10, Omp16, Omp19, Omp2b, and Omp1; native hapten polysaccharide; and lipopolysaccharide and were resistant to complement and polymyxin B at ranges similar to those of the wild-type (WT) counterpart. Likewise, omp3a and omp3b mutants were able to replicate in murine macrophages and in HeLa cells, were resistant to the killing action of human neutrophils, and persisted in mice, like the WT strain. Murine macrophages infected with the omp3a mutant generated slightly higher levels of tumor necrosis factor alpha than the WT, whereas the bvrS mutant induced lower levels of this cytokine. Since the absence of Omp3a or Omp3b does not result in attenuation, it can be concluded that BvrR/BvrS influences additional Brucella properties involved in virulence. Our results are discussed in the light of previous works suggesting that disruption of omp3a generates attenuated Brucella strains, and we speculate on the role of group 3 Omps.
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Paquet, Jean-Yves, Maria A. Diaz, Stephanie Genevrois, Maggy Grayon, Jean-Michel Verger, Xavier De Bolle, Jeremy H. Lakey, Jean-Jacques Letesson, and Axel Cloeckaert. "Molecular, Antigenic, and Functional Analyses of Omp2b Porin Size Variants of Brucella spp." Journal of Bacteriology 183, no. 16 (August 15, 2001): 4839–47. http://dx.doi.org/10.1128/jb.183.16.4839-4847.2001.

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ABSTRACT Omp2a and Omp2b are highly homologous porins present in the outer membrane of the bacteria from the genus Brucella, a facultative intracellular pathogen. The genes coding for these proteins are closely linked in the Brucella genome and oriented in opposite directions. In this work, we present the cloning, purification, and characterization of four Omp2b size variants found in various Brucella species, and we compare their antigenic and functional properties to the Omp2a and Omp2b porins ofBrucella melitensis reference strain 16M. The variation of the Omp2a and Omp2b porin sequences among the various strains of the genus Brucella seems to result mostly from multiple gene conversions between the two highly homologous genes. As shown in this study, this phenomenon has led to the creation of natural Omp2a and Omp2b chimeric proteins in Omp2b porin size variants. The comparison by liposome swelling assay of the porins sugar permeability suggested a possible functional differences between Omp2a and Omp2b, with Omp2a showing a more efficient pore in sugar diffusion. The sequence variability in the Omp2b size variants was located in the predicted external loops of the porin. Several epitopes recognized by anti-Omp2b monoclonal antibodies were mapped by comparison of the Omp2b size variants antigenicity, and two of them were located in the most exposed surface loops. However, since variations are mostly driven by simple exchanges of conserved motifs between the two genes (except for an Omp2b version from an atypical strain of Brucella suisbiovar 3), the porin variability does not result in major antigenic variability of the Brucella surface that could help the bacteria during the reinfection of a host. Porin variation inBrucella seems to result mainly in porin conductivity modifications.
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Bramanti, T. E., and S. C. Holt. "Hemin uptake in Porphyromonas gingivalis: Omp26 is a hemin-binding surface protein." Journal of Bacteriology 175, no. 22 (1993): 7413–20. http://dx.doi.org/10.1128/jb.175.22.7413-7420.1993.

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Farquharson, Kara Louise, Niaya Jackson, Susan Shi Cheng, Ravinder Kaur, and Lea Vacca Michel. "Elucidating the Protein‐Protein Interactions between Nontypeable Haemophilus Influenzae Outer Membrane Proteins Omp26 and Protein D." FASEB Journal 34, S1 (April 2020): 1. http://dx.doi.org/10.1096/fasebj.2020.34.s1.02607.

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Eisenberg, Tobias, Hans-Peter Hamann, Ute Kaim, Karen Schlez, Helga Seeger, Nicole Schauerte, Falk Melzer, et al. "Isolation of Potentially Novel Brucella spp. from Frogs." Applied and Environmental Microbiology 78, no. 10 (March 9, 2012): 3753–55. http://dx.doi.org/10.1128/aem.07509-11.

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ABSTRACTBacterial isolates from frogs were phenotypically identified asOchrobactrum anthropi, but 16S rRNA sequencing showed up to 100% identity withBrucella inopinata. Further analysis ofrecA,omp2a,omp2b,bcsp31, and IS711and multilocus sequence analysis (MLSA) verified a close relationship withBrucella, suggesting the isolates may actually represent novel members of this growing genus of zoonotic pathogens.
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Caro-Hernández, Paola, Luis Fernández-Lago, María-Jesús de Miguel, Ana I. Martín-Martín, Axel Cloeckaert, María-Jesús Grilló, and Nieves Vizcaíno. "Role of the Omp25/Omp31 Family in Outer Membrane Properties and Virulence of Brucella ovis." Infection and Immunity 75, no. 8 (June 11, 2007): 4050–61. http://dx.doi.org/10.1128/iai.00486-07.

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ABSTRACT The genes coding for the five outer membrane proteins (OMPs) of the Omp25/Omp31 family expected to be located in the outer membrane (OM) of rough virulent Brucella ovis PA were inactivated to evaluate their role in virulence and OM properties. The OM properties of the mutant strains and of the mutants complemented with the corresponding wild-type genes were analyzed, in comparison with the parental strain and rough B. abortus RB51, in several tests: (i) binding of anti-Omp25 and anti-Omp31 monoclonal antibodies, (ii) autoagglutination of bacterial suspensions, and (iii) assessment of susceptibility to polymyxin B, sodium deoxycholate, hydrogen peroxide, and nonimmune ram serum. A tight balance of the members of the Omp25/Omp31 family was seen to be essential for the stability of the B. ovis OM, and important differences between the OMs of B. ovis PA and B. abortus RB51 rough strains were observed. Regarding virulence, the absence of Omp25d and Omp22 from the OM of B. ovis PA led to a drastic reduction in spleen colonization in mice. While the greater susceptibility of the Δomp22 mutant to nonimmune serum and its difficulty in surviving in the stationary phase might be on the basis of its dramatic attenuation, no defects in the OM able to explain the attenuation of the Δomp25d mutant were found, especially considering that the fully virulent Δomp25c mutant displayed more important OM defects. Accordingly, Omp25d, and perhaps Omp22, could be directly involved in the penetration and/or survival of B. ovis inside host cells. This aspect, together with the role of Omp25d and Omp22 in the virulence both of B. ovis in rams and of other Brucella species, should be thoroughly evaluated in future studies.
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Khan, M. Nadeem, Ravinder Kaur, and Michael E. Pichichero. "Bactericidal antibody response against P6, protein D, and OMP26 of nontypeableHaemophilus influenzaeafter acute otitis media in otitis-prone children." FEMS Immunology & Medical Microbiology 65, no. 3 (August 2012): 439–47. http://dx.doi.org/10.1111/j.1574-695x.2012.00967.x.

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Dissertations / Theses on the topic "OMP26"

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Kunthalert, Duangkamol, and n/a. "Immunological and structural characterisation of the nontypeable Haemophilus influenzae vaccine protein OMP26." University of Canberra. Health Sciences, 2004. http://erl.canberra.edu.au./public/adt-AUC20060406.101830.

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Nontypeable Haemophilus influenzas (NTHi) is recognised as a significant human pathogen causing mild to severe respiratory tract infections. At present, no vaccine is available for prevention of infection caused by this pathogen. Several outer membrane proteins (OMPs) of NTHi and its lipooligosaccharide have been investigated as possible vaccine antigens against NTHi infections. Previous investigations in our laboratory have shown that OMP26 from an NTHi 289 strain was able to significantly enhance pulmonary clearance of NTHi in a rat model in which animals were immunised via intestinal Peyer's patches and then boosted intratracheally (Kyd and Cripps, 1998; El- Adhami et al., 1999). In recent studies, the OMP26, when used as a parenteral immunogen, was also highly effective at inducing immune responses that led to significantly enhanced clearance of the chinchilla nasopharynx (Kyd et al., 2003). These studies indicate significant potential of the OMP26 as a candidate vaccine antigen and warrant further investigations for development of a vaccine against NTHi. This thesis focussed on the immunological and structural characterisation of the NTHi vaccine candidate, OMP26. Peptides of OMP26 were used as tools to localise the immunologically important regions of the OMP26. Two different E. coli expression systems, the GST gene fusion and the 6xHis tagged systems, were employed to construct the OMP26 peptides. It was found in this study that, despite efforts to optimise the system, the GST-fusion protein system failed to produce consistent results for the purification and storage of the OMP26 peptides. In contrast, the 6xHis tagged system exhibited more reliable outcomes in the production of the recombinant OMP26 peptides and the stability of the stored purified peptides. As such, the purified OMP26 peptides from the 6xHis tagged system were chosen to map major regions of immunological significance for the OMP26 protein. The regions of the OMP26 which are involved in the induction of the acquired immune responses have been identified in the present study. Based on the antigen specific lymphocyte proliferation assay, the dominant T cell epitopes for OMP26 were located between amino acid residues 95 and 197 (T3+T4 region). These identified T cell epitopes exhibited the capability of efficient T cell activation, suggesting that the epitopes within the T3+T4 region potentially had the highest affinity for binding to the MHC molecules than did any other OMP26 region. Using two different assay systems, ELISA and BIA, the predominant B cell epitopes of OMP26 were located between amino acid residues 45 and 145 (T2+T3 region). This region was also found to be immunodominant across all animal species tested, and with all immunisation regimens used. Flow cytometry analysis also revealed that these particular epitopes were expressed on the surface of NTHi cells. By integration of the data obtained from these current experimental studies and the computational analysis of the OMP26 sequence, two hypothetical models of the OMP26 were also proposed in this study. The significant outcomes obtained in this thesis provide a better understanding of the specificity of the host immune responses to the OMP26 protein These findings provide great benefit not only for the development of a future NTHi vaccine but for the development of the peptide-based immunodiagnostic reagents as well. These diagnostic reagents will be valuable, in particular, for the evaluation of efficacy of an NTHi vaccine in humans that may include OMP26 or specific conformational structures. Future studies are still required to further define the minimum epitope length required for the B and T cell responses identified in this study. The significance of these responses in immune protection against NTHi infection also requires further investigations. Human immune responses also need to be determined, but this can only be achieved following clinical trial studies.
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Silva, Maike Paulino da. "OMP29 de Aggregatibacter actinomycetemcomitans: análise filogenética, interação com proteínas de matriz e resposta de células epiteliais." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/42/42132/tde-17082016-100709/.

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OMP29 é uma das principais proteínas de membrana externa de Aggregatibacter actinomycetemcomitans (Aa) e está associada à invasão de célula epitelial gengival (CEG). Os objetivos deste estudo foram: analisar filogeneticamente omp29 e omp29 parálogo (omp29par), em cepas de Aa; determinar a interação de OMP29 com proteínas de matriz extracelular e o efeito da sua interação com CEG, pela avaliação da expressão gênica e produção de mediadores inflamatórios. Variações filogenéticas foram observadas para omp29 e omp29par, bem como para os seus promotores e estas relacionam-se com os sorotipos. A proteína recombinante OMP29his interagiu com fibronectina plasmática e celular (p<0,05), mas não com os domínios F30 e 45 ou com colágenos tipo I, III, IV e V, fibrinogênio, laminina e plasminogênio. A interação das mutantes de Aa deficientes em omp29 e/ou omp29par (obtidas pelo sistema LoxP/Cre) e OMP29his com CEG OBA-09 demonstrou que OMP29 regula positivamente il-18 e negativamente il-6r e il-8 (p<0,05). Os dados sugerem que OMP29 está envolvida na evasão do sistema imune.
OMP29 is one of the major outer membrane proteins of Aggregatibacter actinomycetemcomitans (Aa) and is associated with invasion into gingival epithelial cells (GEC). This study aimed to evaluate phylogenetically omp29 and omp29 paralogue (omp29par) in Aa strains; determine the interaction of OMP29 with extracellular matrix proteins and its effect on GEC by gene expression analysis and production of inflammatory mediators. Phylogenetic variations were observed for omp29 and omp29par as well as their promoters, and they were related to the serotypes. The recombinant protein OMP29his interacted with plasma and cellular fibronectin (p <0.05), but not to F30 and F45 domains, neither to collagens I, III, IV and V, fibrinogen, laminin and plasminogen. The interaction of Aa mutants defective in omp29 and/or omp29par (obtained by LoxP system/Cre) and OMP29his with CEG OBA-09 indicated that OMP29 regulates positively il-18 and negatively il-6r and il-8 (p <0.05). Data suggested that OMP29 is involved in bacterial evasion of the immune system.
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Degos, Clara. "Contrôle et modulation de la réponse immunitaire par Brucella abortus." Thesis, Aix-Marseille, 2014. http://www.theses.fr/2014AIXM4069/document.

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Brucella est une bactérie pathogène intracellulaire responsable d'une maladie, la brucellose. Sa capacité à établir une infection chronique est due aux mécanismes qu'elle déploie pour inhiber la réponse immunitaire. Parmi les cellules infectées, les cellules dendritiques (DC) et les macrophages (MO) jouent un rôle primordial dans l'induction de la réponse immunitaire. Nous avons étudié le rôle d'un récepteur des DC, des lymphocytes T (LT), MO : CD150. Il participe à l'activation des LT et il a été montré que CD150 est capable de reconnaître des protéines de l'enveloppe bactérienne, ce qui conduit à une augmentation de son expression à la membrane des MO. Nous avons découvert que l'expression de CD150 sur les DC dérivées de moelle osseuse (BMDC) augmente en présence d'extraits membranaire de Brucella, sauf quand ces derniers proviennent d'un mutant pour Omp25 (∆omp25). L'infection de BMDC par ∆omp25 active les BMDC en termes d'expression de molécules de co-stimulation, d'ARNm pro-inflammatoires (cytokines) et de translocation de NF-κB dans le noyau. En comparant avec une souche sauvage de Brucella, l'activation par ∆omp25 est plus importante. En absence de CD150 la translocation de NF-κB dans les BMDC infectées par la souche sauvage est aussi importante que celle induite par l'infection par ∆omp25. In vivo CD150 contrôle la réplication de Brucella dans la rate de souris infectées. Nous avons démontré que CD150 est capable de lier Omp25. Nous avons identifié un nouveau mécanisme par lequel Brucella inhibe l'activation des DC : la liaison d'Omp25 à CD150. Ce récepteur joue un double rôle puisqu'il est aussi crucial dans le contrôle de la survie de Brucella in vivo
Brucella is a pathogenic intracellular bacterium responsible for a disease, brucellosis. The ability of Brucella to establish a chronic infection is linked to the mechanism it uses to inhibit immune response. Among Brucella infected cells, dendritic cells (DC) and macrophages play a crucial role in the induction of an immune response. We studied the role of one receptor present at the DC, T cells, macrophages surface: CD150. This molecule participates at the T cell activation and it was shown recently that CD150 can recognize bacterial membrane proteins which lead to its own upregulation. We discovered that CD150 expression onto bone-marrow derived DC (BMDC) is increased when these cells are treated with Brucella membrane extracts, except when those extracts are coming from a mutant for Omp25(∆omp25), a Brucella membrane protein. BMDC infection with ∆omp25 leads to BMDC activation regarding co-stimulatory molecule expression, cytokines and chemokines secretion, pro-inflammatory mRNA expression and NF-κB translocation within the nucleus. In comparison with infection with the wild type strain, ∆omp25 induces a higher activation of BMDC. In absence of CD150, NF-κB translocation within the nucleus of infected-BMDC is the same between the wild type strain and ∆omp25. In vivo, CD150 controls Brucella replication in the spleen of infected mice, and Omp25 infection seems to trigger a higher inflammation in control mice. We finally demonstrate that CD150 binds Omp25. Here, we identified a new mechanism by which Brucella is able to inhibit DC activation: binding of Omp25 to CD150. This receptor plays a dual role since it is also required for controlling Brucella growth in mice
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Billard, Elisabeth. "Etude de l'impact de l'infection à Brucella sur la physiologie des cellules dendritiques humaines." Montpellier 2, 2007. http://www.theses.fr/2007MON20045.

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Les bactéries appartenant au genre Brucella sont des pathogènes intracellulaires responsables de la brucellose (fièvre de Malte). La clé de leur virulence réside dans leur capacité à envahir les cellules phagocytaires et à proliférer dans les phagosomes en échappant au système immunitaire. . .
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Dahlstrand, Rudin Arvid, and John Burstedt. "The importance of OuterMembrane Protein A in SerumResistance in Aggregatibacteractinomycetemcomitans serotype astrain D7SS." Thesis, Umeå universitet, Institutionen för odontologi, 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-143904.

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The Gram-negative bacterium Aggregatibacter actinomycetemcomitans is primarily associatedwith aggressive forms of periodontal disease. Additionally, it has occasionally been found to causemetastatic infections in non-oral sites. This requires the ability to evade the bactericidal activity ofthe complement system of the humoral immune system. Outer membrane proteins, namely,Omp100 and OmpA have been connected to normal human serum resistance for several bacteriaspecies. The objective of this study was to investigate if serum-resistant ompA mutants can beobtained, and to detect changes in OMP expression. We used A. actinomycetemcomitansserotype a strain D7SS and D7SS ompA knockouts. The strains were incubated in 50 % NHS.This resulted in a substantial decrease of survival among D7SS ompA knockouts. D7SS ompAknockouts were exposed to 50 % NHS once more to confirm stable serum resistance. 13 out of14 tested clones showed growth, indicating that serum resistant ompA mutants could begenerated. SDS-PAGE gel of extracted outer membrane vesicles revealed an additional proteinband of approximately 34 kDa in at least 4 of 5 tested serum resistant ompA mutants. This proteinband has been analyzed in the laboratory, and according to LC-MS/MS it contains an OmpAhomologue, which has been named OmpA2. We conclude that OmpA2 expression might be amajor mechanism for serum survival in A. actinomycetemcomitans serotype a strain D7SS ompAknockouts.
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Chiang, Kun-Lin, and 蔣昆霖. "Studying on the ribosomal protein L5 and outer membrane protein OMP2." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/b625ef.

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碩士
國立成功大學
生物化學研究所
90
L5 is an 894bp, 34KDa ribosomal protein that is a part of 60S ribosomal subunit and localizes in both the cytoplasm and the nucleus of eukaryotic cells, accumulating particularly in the nucleoli. L5 is known to bind specifically to 5S rRNA and is involved in nucleocytoplasmic transport of this rRNA. In mammalian cells,only about half of the L5 are in 60S ribosome and the other half of the L5 are bound by 5S rRNA to form an 5S RNP in the nucleus. L5 can also be associated with type Ⅰphosphatase, CKⅡ (casein kinase Ⅱ) and oncoprotein mdm2. L5 may be involved in other intracellular regulatory mechanism. Previous reports indicated that phosphorylation of RPA2 prevents the association of RPA with p53 , and L5 is associated with mdm2 and mdm2-p53 complexes. Also, in our previous study, we discovered that L5 interactes with hyperphosphorylated form of RPA2, but not with hypophorylated form of RPA2, when cells was irradiated with UV. This raises an interesting possibility that L5 might directly associate with p53 in unirradiated cells, and upon UV irradiation, it releases the bound p53, thus transducing the damage signal and activating the p53-dependent checkpoint control. To test this hypothesis, S35-radiolabled L5 and p53 proteins were prepared by coupled in vitro transcription / translation and were subjected to immunoprecipitation analysis with anti-6Histidine antibody. The results showed that L5 can associate with p53 directly. Further study to determine which domain of p53 for L5 binding was proceeded. The results revealed that L5 can bind at the carboxyl terminus of the p53 protein. Klebsiella pneumoniae is the common pathogen of nosocomial and community-acquired infections, especially bacterium, pneumonia, and urinary tract infections. In Taiwan, the high incidence of K. pneumoniae pyogenic liver abscess has been reported which never reported in English literature during the period from 1980 (30%) to 1990 (82.1%). The exact mechanisms of increasing incidence of K. neumoniae pyogenic liver abscess in Taiwan is unclear. In our previous study, a gene encoding outer membrane protein(OMP2) has been cloned from a highly virulent strain of K.p.129 ( LD50 < 102 c.f.u.), and anti-OMP2 antibody has a significantly protective effect when administered before acute lethal infection. Thus, correlation of OMP2 with respect to the virulence of K. pneumoniae was examinied in this study. Several isogenic OMP2-deficient strains were constructed by using integrational plasmid to disrupt the omp2 gene. The deficient strains checked by PCR and southern blotting, which was further examined by LD50 test. The results indicate that OMP2 may be not play a more important role in the pathogenesis of K. pneumoniae.
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Hsieh, Yi-Ping, and 謝一平. "Correlation between imipenem resistance of Acinetobacter baumannii and expression of β-lactamase OXA-66 and outer membrane protein Omp25." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/38439926752144532103.

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碩士
國立陽明大學
醫學生物技術研究所
94
Our laboratory previously demonstrated that overexpression of OXA-51-like carbapenemase and outer membrane protein (Omp) 25 in imipenem-resistant strains of Acinetobacter baumannii. The primers were designed to amplify this OXA-51-like carbapenemase encoded gene. DNA sequence analysis revealed this enzyme is OXA-66 carbapenemase. Surprisingly, the presence of blaoxa-66 and it’s closely related variants’ gene was shown in all tested clinical strains of A. baumannii. Comparing blaoxa-66 gene sequence, between the imipenem susceptible strain AB1254 and the imipenem resistant strain AB1266, there was a single nucleotide change from C to G at nucleotide position 499 in AB1266. This replacement resulted in the amino acid residue change from leucine to valine. Furthermore, oxa-66 mRNA level was significantly higher in imipenem-resistant strains than those of imipenem-susceptible strains by RT-PCR and real-time RT-PCR assays. In order to illustrate the correlation between the OXA-66 and imipenem resistance, we constructed the expression vector carrying the OXA-66 encoded gene (AB1266-oxa-66) from the imipenem resistant strain AB1266 and the same gene from the imipenem susceptible strain AB1254 respectively. Overexpression the blaoxa-66 gene from AB1266 and AB1254 in E. coli system, and their minimal inhibitory concentrations (MIC) were determined by the Etest. The results showed that overexpression of the AB1266-OXA-66 elevated 7- to 8-folds for the resistance to imipenem, and overexpression of the AB1254-OXA-66 also elevated 3- to 4-folds for the resistance to imipenem in E. coli. We also found overexpression of OXA-66 was toxic to E. coli. In Taiwan, there is no report yet to demonstrate the correlation between expression of blaoxa-66 and imipenem resistance in A. baumannii. This is the first study to provide the evidence to declare their correlation.
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Conference papers on the topic "OMP26"

1

Okpowo, Blessyn, Ebenezer Ageh, Peter Agodo, Akpan Okon, Bassey Mfon, and Tata Emmanuel. "Gains of an Effective Community Management Framework: The OML26 Experience." In SPE Nigeria Annual International Conference and Exhibition. Society of Petroleum Engineers, 2019. http://dx.doi.org/10.2118/198802-ms.

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2

Cho, K. Y., J. H. Chang, B. S. Choi, Y. Takushima, and Y. C. Chung. "Demonstration of 25.78-Gb/s, 20-km Reach WDM PON Using Directly-Modulated Bandwidth-Limited RSOA." In Optical Fiber Communication Conference. Washington, D.C.: OSA, 2011. http://dx.doi.org/10.1364/ofc.2011.omp2.

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3

Van Campenhout, Joris, William M. J. Green, Solomon Assefa, and Yurii A. Vlasov. "Ultra-Broadband, Low-Power, 2×2 Electro-Optic Switch using Sub-Micron Silicon Waveguides." In Optical Fiber Communication Conference. Washington, D.C.: OSA, 2010. http://dx.doi.org/10.1364/ofc.2010.omp2.

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4

Yeo, Yong-Kee, Zhaowen Xu, Chin-Yi Liaw, Dawei Wang, Yixin Wang, and Tee-Hiang Cheng. "A 448 × 448 Optical Cross-Connect for High-Performance Computers and Multi-Terabit/s Routers." In Optical Fiber Communication Conference. Washington, D.C.: OSA, 2010. http://dx.doi.org/10.1364/ofc.2010.omp6.

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5

Zhang, Bo, X. Steve Yao, Xiaojun Chen, and Alan E. Willner. "Polarization-based Fast-Swept Optical Spectrum Analyzer." In Optical Fiber Communication Conference. Washington, D.C.: OSA, 2009. http://dx.doi.org/10.1364/ofc.2009.omp2.

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6

Okamoto, Keiji, and Fumihiko Ito. "Demonstration of Digital Wavelength Demultiplexing for Group Delay Dispersion Measurement." In Optical Fiber Communication Conference. Washington, D.C.: OSA, 2009. http://dx.doi.org/10.1364/ofc.2009.omp6.

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7

Ajao, Wale, Ezinwanneakolam Isiba, Eddy Okoh, Immaculate Okoruwa, Stanley Omenai, Nicholas Abu, Olusegun Babalola, and Oluwatobi Oke. "Production Optimization Strategies for Unlocking Resource Potential in A Heavy-Oil Brown Field - The OML26 Success Story." In SPE Nigeria Annual International Conference and Exhibition. Society of Petroleum Engineers, 2019. http://dx.doi.org/10.2118/198800-ms.

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8

Ageh, Ebenezer, Ezinwanneakolam Isiba, Immaculate Okoruwa, Wale Ajao, Idalla Yebusika, Amos Onopkise, Oluwatobi Oke, Victor Agbaroji, and Willie Okon. "Unlocking Potential in Mature Brown Field with Complex Configuration in the Niger Delta- The OML26 Success Story." In SPE Nigeria Annual International Conference and Exhibition. Society of Petroleum Engineers, 2020. http://dx.doi.org/10.2118/203655-ms.

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9

Acha, Smart, James Fadairo, Dennis Ogiesoba, Ikenna Okeke, Tata Emmanuel, and Joseph A. Brown. "Achieving & Sustaining Impeccable HSE Performance in Brown Field Operations Comes at a Cost OML26 Blazes the Trail." In SPE Nigeria Annual International Conference and Exhibition. Society of Petroleum Engineers, 2019. http://dx.doi.org/10.2118/198801-ms.

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