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Hwang, K., M. Dubois, D. K. Panda, S. Rao, S. Shang, A. Uresin, W. Mao, et al. "OMP." ACM SIGARCH Computer Architecture News 18, no. 3b (September 1990): 7–22. http://dx.doi.org/10.1145/255129.255133.
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Wu, Ting-Huai, and Xin-Xing Gu. "Outer Membrane Proteins as a Carrier for Detoxified Lipooligosaccharide Conjugate Vaccines for NontypeableHaemophilus influenzae." Infection and Immunity 67, no. 10 (October 1, 1999): 5508–13. http://dx.doi.org/10.1128/iai.67.10.5508-5513.1999.
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ABSTRACT Nontypeable Haemophilus influenzae (NTHi) is a common cause of otitis media and respiratory tract infections. Outer membrane proteins (OMP) and lipooligosaccharide (LOS) are major surface antigens of NTHi and potential vaccine candidates. De-O-acylated LOS (dLOS) or oligosaccharide (OS) was coupled to total OMP to form dLOS-OMP and OS-OMP conjugates, while a dLOS-tetanus toxoid (TT) was synthesized for comparison. These conjugates were evaluated in mice and rabbits for immunogenicity. dLOS-OMP elicited a better boostable antibody response against LOS than did dLOS-TT, while OS-OMP was not immunogenic. Formulation of the conjugates with Ribi adjuvant significantly enhanced the immunogenicity of dLOS-OMP and dLOS-TT but not that of OS-OMP. In addition, rabbit antisera elicited by dLOS-OMP but not dLOS-TT (or OMP alone) demonstrated bactericidal activity against 40% of the NTHi strains tested. These results indicate that dLOS is a better derivative of LOS than OS and that OMP is a good carrier for NTHi LOS-based conjugate vaccines.
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Øksendal, A. N., T. Bach-Gansmo, T. Flem Jacobsen, H. Eide, and E. Andrew. "Oral Magnetic Particles." Acta Radiologica 34, no. 2 (March 1993): 187–93. http://dx.doi.org/10.1177/028418519303400217.
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Oral magnetic particles (OMP) have been evaluated in a clinical phase II trial program comprising 216 patients in 7 European centers. Adult patients referred for MR imaging for various abdominal pathologies were examined. The patients received OMP at a concentration of 0.1 g/l (ultralow field) or 0.5 g/l (mid/high field) and OMP was diluted in water or in a more viscous liquid formulation. Depending on the area of interest, OMP was ingested in a volume of 300 to 800 ml. OMP was well tolerated with no serious adverse events and the patient acceptability was good. OMP had a good contrast effect on all applied pulse sequences. The viscous formulation of OMP was homogeneously distributed through the entire gastrointestinal tract without inducing disturbing susceptibility artifacts. The postcontrast diagnostic information was improved in 70% of the cases. Based on the encouraging results in phase II, OMP has been advanced to phase III clinical trials.
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Erwin, Alice L., George E. Kenny, Arnold L. Smith, and Terrence L. Stull. "Human antibody response to outer membrane proteins and fimbriae of Haemophilus influenzae type b." Canadian Journal of Microbiology 34, no. 6 (June 1, 1988): 723–29. http://dx.doi.org/10.1139/m88-123.
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We investigated the prevalence of antibodies in childrens' sera directed against outer membrane proteins (OMP) and fimbriae of Haemophilus influenzae type b. Invasive isolates of H. influenzae type b were enriched for fimbriae production; OMP and fimbriae were resolved by SDS-PAGE. After blotting to nitrocellulose, the proteins were incubated with homologous patient sera or with sera from healthy children. IgG antibodies bound to OMP were detected by immunoperoxidase staining. Immunoblotting was also performed using purified, nondenatured fimbriae as antigen. Nine of the 10 patients studied had antibodies in the acute serum directed against one or more of the OMP. Neither the acute nor the convalescent serum of the remaining patient contained antibodies against OMP. Antibodies against a greater number of OMP were present in the convalescent serum, in comparison to the acute serum, in 4 of the 10 patients. Five of 10 patients had antibodies against the purified fimbriae of an unrelated invasive isolate in either the acute or the convalescent serum. Acute sera from patients more frequently contained antibodies directed against OMP 60K (p ≤ 0.01) and OMP 51K (p ≤ 0.003) compared with the sera of healthy controls. In contrast, the sera of healthy children more frequently contained antibodies directed against OMP 40K (p ≤ 0.04). Sera from both patients and controls contained antibodies against commensal Haemophilus. We conclude that although antibodies against OMP are commonly present in healthy children, antibodies against certain OMP may be markers for susceptibility or protection.
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Zhang, Chunbin, Qingming Xiong, Takane Kikuchi, and Yasuko Rikihisa. "Identification of 19 Polymorphic Major Outer Membrane Protein Genes and Their Immunogenic Peptides in Ehrlichia ewingii for Use in a Serodiagnostic Assay." Clinical and Vaccine Immunology 15, no. 3 (December 19, 2007): 402–11. http://dx.doi.org/10.1128/cvi.00366-07.
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ABSTRACT Ehrlichia ewingii, a tick-transmitted rickettsia previously known only as a canine pathogen, was recently recognized as a human pathogen. E. ewingii has yet to be cultivated, and there is no serologic test available to diagnose E. ewingii infection. Previously, a fragment (505 bp) of a single E. ewingii gene homologous to 1 of 22 genes encoding Ehrlichia chaffeensis immunodominant major outer membrane proteins 1 (OMP-1s)/P28s was identified. The purposes of the present study were to (i) determine the E. ewingii omp-1 gene family, (ii) determine each OMP-1-specific peptide, and (iii) analyze all OMP-1 synthesized peptides for antigenicity. Using nested touchdown PCR and a primer walking strategy, we found 19 omp-1 paralogs in E. ewingii. These genes are arranged in tandem downstream of tr1 and upstream of secA in a 24-kb genomic region. Predicted molecular masses of the 19 mature E. ewingii OMP-1s range from 25.1 to 31.3 kDa, with isoelectric points of 5.03 to 9.80. Based on comparative sequence analyses among OMP-1s from E. ewingii and three other Ehrlichia spp., each E. ewingii OMP-1 oligopeptide that was predicted to be antigenic, bacterial surface exposed, unique in comparison to the other E. ewingii OMP-1s, and distinct from those of other Ehrlichia spp. was synthesized for use in an enzyme-linked immunosorbent assay. Plasmas from experimentally E. ewingii-infected dogs reacted significantly with most of the OMP-1-specific peptides, indicating that multiple OMP-1s were expressed and immunogenic in infected dogs. The results support the utility of the tailored OMP-1 peptides as E. ewingii serologic test antigens.
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Mahelingga, Dhevi Enlivena Irene Restia. "Penerbitan buku ilmiah daring berbasis open monograph press (OMP)." Berkala Ilmu Perpustakaan dan Informasi 16, no. 2 (December 1, 2020): 155–69. http://dx.doi.org/10.22146/bip.v16i2.265.
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Introduction. There has been limited discussion on Open Monograph Press (OMP) although its potential is recognized to support academic publications in Indonesia. This is due to the lack of access to information and dissemination of OMP by academic publishers that have implemented OMP in their publication process.
Data Collection Method. This paper used a qualitative approach by starting the elaboration publication process in Lembaga Ilmu Pengetahuan Indonesia (LIPI) Press. This paper examined how OMP-based online publishing system is able to accommodate book publishing process in LIPI Press. Primary data in this study was collected through records of the user interface of the online page of LIPI Press which uses OMP.
Data Analysis. The data was analyzed by using a descriptive-analytic approach.
Results and Discussions. OMP is useful not only to accommodate all roles and processes of scientific publication but also to accommodate the publisher's need for a catalog website. OMP metadata can be indexed by Google Scholar and can be a helpful tool for product distribution.
Conclusion. OMP is suggested to become a standard in publication process.
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Shahin, R. D., M. J. Brennan, Z. M. Li, B. D. Meade, and C. R. Manclark. "Characterization of the protective capacity and immunogenicity of the 69-kD outer membrane protein of Bordetella pertussis." Journal of Experimental Medicine 171, no. 1 (January 1, 1990): 63–73. http://dx.doi.org/10.1084/jem.171.1.63.
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Immunization with the 69-kD outer membrane protein (OMP) of Bordetella pertussis protected neonatal mice against lethal respiratory challenge with B. pertussis 18323. Active immunization elicited a serum IgG anti-69-kD OMP response at the time of challenge, with IgG anti-69-kD OMP antibodies detected in bronchoalveolar lavage fluid after challenge. Intravenous administration of BPE8, a monoclonal IgG1 anti-69-kD OMP, also protected young mice against B. pertussis challenge. Intravenously injected BPE8 was detected in the lungs of mice at the time of aerosol challenge, suggesting that the presence of specific antibody in the lungs may mediate protection. Thus the 69-kD OMP of B. pertussis is a protective antigen in mice that elicits specific serum antibody that can transude to the lung. The 69-kD OMP was detected in a preparation of a Takeda acellular vaccine by immunoblot analysis and a serum antibody response to the 69-kD OMP was observed in 18-mo-old children boosted with this preparation of Japanese acellular vaccine. Our results demonstrate that the B. pertussis 69-kD OMP is a protective antigen in animals, is immunogenic in humans, and is present in a preparation of acellular pertussis vaccine that is widely used in Japan. These findings indicate that the 69-kD OMP should be seriously considered as a candidate for inclusion in new formulations of antigenically defined acellular pertussis vaccines.
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Adlowitz, Diana G., Sanjay Sethi, Paul Cullen, Ben Adler, and Timothy F. Murphy. "Human Antibody Response to Outer Membrane Protein G1a, a Lipoprotein of Moraxella catarrhalis." Infection and Immunity 73, no. 10 (October 2005): 6601–7. http://dx.doi.org/10.1128/iai.73.10.6601-6607.2005.
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ABSTRACT Moraxella catarrhalis is an important cause of respiratory infections in adults with chronic obstructive pulmonary disease (COPD) and of otitis media in children. Outer membrane protein (OMP) G1a is an ∼29-kDa surface lipoprotein and is a potential vaccine candidate. The gene that encodes OMP G1a was expressed and purified using a novel plasmid vector. [3H]palmitic acid labeling demonstrated that both native and recombinant OMP G1a contain covalently bound palmitic acid. To assess the expression of OMP G1a during human infection, paired sera and sputum supernatants from adults with COPD followed prospectively were studied by enzyme-linked immunosorbent assays with recombinant lipidated OMP G1a to detect antibodies made specifically during carriage of M. catarrhalis. Overall, 23% of patients developed either a serum immunoglobulin G (IgG) response (9%) or sputum IgA response (21%) to OMP G1a, following 100 episodes of acquisition and clearance of M. catarrhalis. Patients developed antibody responses at similar rates following episodes of clinical exacerbation compared to asymptomatic colonization. Serum IgG antibodies following natural infection were directed predominantly at OMP G1a epitopes that are not exposed on the bacterial surface. These data show that OMP G1a is expressed during infection of the human respiratory tract and is a target of systemic and mucosal antibodies. These observations indicate that OMP G1a, a highly conserved surface protein, should be evaluated further as a vaccine candidate.
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Milner, Nicholas P., Michael R. Johnson, and Kim J. Perry. "Determination of Sulfadimethoxine and Ormetoprim Residues in Channel Catfish Fillets After Treatment with Romet and Evaluation of a Commercially Available Rapid Diagnostic Test for Drug Residues in Fish Fillets." Journal of AOAC INTERNATIONAL 77, no. 4 (July 1, 1994): 875–81. http://dx.doi.org/10.1093/jaoac/77.4.875.
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Abstract Sulfadimethoxine (SDM) and ormetoprim (OMP) residues were determined in catfish fillets after treatment with Romet. A liquid chromatographic (LC) method capable of simultaneously detecting SDM and OMP at 0.05–40 ppm was used. The recoveries were 92% for SDM and 108% for OMP. The intra- assay variabilities were determined for SDM and OMP at fortification levels of 0.05–40 ppm, and a coefficient of variation (CV) of less than 10% was achieved at all levels. The interassay variations were determined at fortification levels of 0.1 ppm (CVs: SDM, 6.4%; OMP, 4.9%) and 1.0 ppm (CVs: SDM, 2.8%; OMP, 3.5%). SDM and OMP residues in catfish fillets were rapidly depleted after treatment with Romet. By day 2 posttreatment, SDM and OMP were essentially nondetectable. The use of an enzyme-linked immunoassay (ELISA)-based rapid diagnostic test to determine SDM and OMP in catfish fillets was evaluated. An assay procedure to adapt the test for residues in tissue samples was developed. SDM and OMP were extracted from ground catfish fillet with methanol–water (80 + 20, v/v); the extract was diluted with buffer and filtered, and the filtrate was then tested with an EZ Screen test card. The effectiveness of the rapid test assay to detect SDM residues at levels above the U.S. Food and Drug Administration tolerance (0.1 ppm) was verified with fillets from Romet-treated catfish. All samples with residues at >0.1 ppm were identified correctly by the test. The results indicate that the diagnostic test could be used as a rapid method for monitoring Romet residues in catfish.
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Berenson, Charles S., Timothy F. Murphy, Catherine T. Wrona, and Sanjay Sethi. "Outer Membrane Protein P6 of Nontypeable Haemophilus influenzae Is a Potent and Selective Inducer of Human Macrophage Proinflammatory Cytokines." Infection and Immunity 73, no. 5 (May 2005): 2728–35. http://dx.doi.org/10.1128/iai.73.5.2728-2735.2005.
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ABSTRACT Interactions of nontypeable Haemophilus influenzae (NTHI) with human macrophages contribute to the pathogenesis of NTHI-induced infection in humans. However, the immunologic mechanisms that initiate and perpetuate NTHI-mediated macrophage responses have not been well explored. Outer membrane protein (OMP) P6 is a conserved lipoprotein expressed by NTHI in vivo that possesses a Pam3Cys terminal motif, characteristic of immunoactive bacterial lipoproteins associated with Toll-like receptor signaling. We theorized that OMP P6 is a potent immunomodulator of human macrophages. To test this hypothesis, we purified OMP P6 as well as OMP P2, the predominant NTHI outer membrane protein, and lipooligosaccharide (LOS), the specific endotoxin of NTHI, from NTHI strain 1479. Human blood monocyte-derived macrophages, purified from healthy donors, were incubated with each outer membrane constituent, and cytokine production of macrophage supernatants interleukin-1β (IL-1β), tumor necrosis factor α (TNF-α), IL-10, IL-12, and IL-8 was measured. OMP P6 selectively upregulated IL-10, TNF-α, and IL-8. While OMP P6 (0.1 μg/ml for 8 h) elicited slightly greater concentrations of IL-10, it resulted in over ninefold greater concentrations of TNF-α and over fourfold greater concentrations of IL-8 than did OMP P2. OMP P6 at doses as low as 10 pg/ml was still effective at induction of macrophage IL-8, while OMP P2 and LOS were not. OMP P6 of NTHI is a specific trigger of bacteria-induced human macrophage inflammatory events, with IL-8 and TNF-α as key effectors of P6-induced macrophage responses.
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Surikova, Ekaterina Igorevna, Galina Nerodo, Irina Aleksandrovna Goroshinskaya, and Polina Sergeevna Kachesova. "Oxidative modification of proteins of blood plasma in the development of ovarian cancer relapse." Journal of Clinical Oncology 31, no. 15_suppl (May 20, 2013): e22002-e22002. http://dx.doi.org/10.1200/jco.2013.31.15_suppl.e22002.
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e22002 Background: Comparative analysis of oxidative modification of proteins of blood plasma level in patients with ovarian cancer in a state of remission and during the formation of tumor relapse with various intensity of bloodstream in it. Methods: The research includes 61 patients with ovarian cancer, from whom 11 women were in a state of remission, and 50 had local relapse in the cavity of the lesser pelvis. With the help of ultra-sonic scanner we evaluated the intensity of vascularization and the speed of bloodstream in relapsing tumors. We also studied the level of blood plasma spontaneous metal catalyzed oxidative modification of proteins (OMP), spectrophotometrically registering 2, 4-dinitrophenylhydrazines. Results: In remission there was noted the increase of OMP products level by 74.4% under 530 nm (p<0.05) in blood plasma as compared with the its level in healthy women. During the progress of avascular relapse there was noted the increase of OMP products level by 63.3% under 370 nm (p<0.001) and decrease of OMP products level by 32.2% under 530 nm as compared with the level in remission. In the group with hyper intensive bloodstream in relapse tumor the level of OMP products increased by 37.4% under 370nm (p<0.05) and the level of products decreased by 24% under 530 nm as compared with the group with avascular relapse. In case with metal catalyzed OMP the low level of OMP products under 370 nm (by 18-28% lower than the level of OMP of healthy women, p<0.01), the high level of OMP products under 530 nm (by 24-47% higher than the level of OMP of healthy women, p<0.01) remained, and the level of OMP under 430 nm was increasing as compared with healthy women. Conclusions: The increase of OMP products of neutral character registered under 370 nm, and the decrease of the products of the basic character registered under 530 nm take place during the progress of cancerous process and intensification of the bloodstream in a tumor. The dynamics metal catalyzed OMP products shows the heightened formation of the products of the basic character (430 and 530 nm), being the markers of proteins fragmentation.
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Nugraha, J., and Rahayu Anggraini. "ANTIGEN OMP (OUTER MEMBRANE PROTEIN) salmonella typhi FAGA LOKAL YANG IMUNODOMINAN DAN SPESIFIK TERHADAP ANTIBODI PENDERITA DEMAM TIFOID." INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY 13, no. 3 (March 15, 2018): 109. http://dx.doi.org/10.24293/ijcpml.v13i3.912.
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Background of this research is that diagnostics of typhoid fever is still a health problem. Widal test, which is the mostly used test in Indonesia, frequently gives false positive results and is not reliable in endemic areas. On the other hand, the other confirmation test, bloodculture, is not sensitive and often give false negative results. OMP (Outer Membrane Protein) is known as a specific part of Salmonellatyphi and fragments of OMP still exist in the patient’s body since early infection until 2–3 weeks thereafter. In this study parts of OMPwhich react specifically with sera of typhoid fever in Indonesia were searched. These specific parts will then be developed as a diagnostickit for typhoid fever. Using Western Blot method, part of OMP will be searched, which is specifically react with sera of typhoid feverpatients in Indonesia. OMP derived from local phage type isolated in Indonesia was used. This OMP was separated with SDS-PAGE 12%and incubated with pooled sera of typhoid fever patients, and sera of control group, that is from Dengue haemorrhagic fever patientsand urinary tract infection with E. coli. Extraction of OMP was done by the method of Matsuyama. Contrary, this research failed to find a particular part of OMP which react specifically with sera of typhoid fever patients. There are certain parts of OMP which react also with sera of DHF & urinary tract infection patients. Our finding was different with the results from Malaysia, where it is reported thatantigen OMP 52 kD react specifically there. In order to develop a diagnostic tool for typhoid fever, we should consider another possiblespecific antigen other rather than using OMP.
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Rosenqvist, Einar, Alexis Musacchio, Audun Aase, E. Arne Høiby, Ellen Namork, Jan Kolberg, Elisabeth Wedege, et al. "Functional Activities and Epitope Specificity of Human and Murine Antibodies against the Class 4 Outer Membrane Protein (Rmp) of Neisseria meningitidis." Infection and Immunity 67, no. 3 (March 1, 1999): 1267–76. http://dx.doi.org/10.1128/iai.67.3.1267-1276.1999.
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ABSTRACT Antibodies against the class 4 outer membrane protein (OMP) fromNeisseria meningitidis have been purified from sera from vaccinees immunized with the Norwegian meningococcal group B outer membrane vesicle vaccine. The human sera and purified antibodies reacted strongly with the class 4 OMP in immunoblots, whereas experiments with whole bacteria showed only weak reactions, indicating that the antibodies mainly reacted with parts of the class 4 molecule that were not exposed. The purified human anti-class 4 OMP antibodies and the monoclonal antibodies (MAbs) were neither bactericidal nor opsonic against live meningococci. Three new MAbs against the class 4 OMP were generated and compared with other, previously described MAbs. Three linear epitopes in different regions of the class 4 OMP were identified by the reaction of MAbs with synthetic peptides. The MAbs showed no blocking effect on bactericidal activity of MAbs against other OMPs. However, one of the eight purified human anti-class 4 OMP antibody preparations, selected from immunoblot reactions among sera from 27 vaccinees, inhibited at high concentrations the bactericidal effect of a MAb against the class 1 OMP. However, these antibodies were not vaccine induced, as they were present also before vaccination. Therefore, this study gave no evidence that vaccination with a meningococcal outer membrane vesicle vaccine containing the class 4 OMP induces blocking antibodies. Our data indicated that the structure of class 4 OMP does not correspond to standard β-barrel structures of integral OMPs and that no substantial portion of the OmpA-like C-terminal region of this protein is located at the surface of the outer membrane.
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Murphy, Timothy F., Charmaine Kirkham, Ernesto DeNardin, and Sanjay Sethi. "Analysis of Antigenic Structure and Human Immune Response to Outer Membrane Protein CD of Moraxella catarrhalis." Infection and Immunity 67, no. 9 (September 1, 1999): 4578–85. http://dx.doi.org/10.1128/iai.67.9.4578-4585.1999.
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ABSTRACT Moraxella catarrhalis is an important cause of otitis media in children and lower respiratory tract infections in adults with chronic obstructive pulmonary disease (COPD). Outer membrane protein CD (OMP CD) is a 45-kDa protein which is a potential vaccine antigen to prevent infections caused by M. catarrhalis. Eight monoclonal antibodies were used to study the antigenic structure of the OMP CD molecule by assaying recombinant peptides corresponding to the sequence of the protein. This approach identified two surface-exposed epitopes, including one near the amino terminus (amino acids 25 to 44) and one in the central region of the molecule (amino acids 261 to 331). Assays with serum and sputum supernatants of adults with COPD revealed variable levels of antibodies to OMP CD among individuals. To determine which portions of the OMP CD molecule were recognized by human antibodies, three human serum samples were studied with six recombinant peptides which span the sequence of OMP CD. All three sera contained immunoglobulin G antibodies which recognized exclusively the peptide corresponding to amino acids 203 to 260 by immunoblot assay. Adsorption experiments with whole bacteria established that some of the human antibodies are directed at surface-exposed epitopes on OMP CD. We conclude that OMP CD is a highly conserved molecule which contains at least two separate epitopes which are exposed on the bacterial surface. While individual adults with COPD show variability in the immune response to OMP CD, a specific region of the OMP CD molecule (amino acids 203 to 260) is important as a target of the human immune response.
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O'Reilly, Eileen Mary, Lon S. Smith, Johanna C. Bendell, Fatima A. Rangwala, William Schmidt, Joe Stephenson, Ann Kapoun, et al. "Phase Ib of anticancer stem cell antibody OMP-59R5 (anti-Notch2/3) in combination with nab-paclitaxel and gemcitabine (Nab-P+Gem) in patients (pts) with untreated metastatic pancreatic cancer (mPC)." Journal of Clinical Oncology 32, no. 3_suppl (January 20, 2014): 220. http://dx.doi.org/10.1200/jco.2014.32.3_suppl.220.
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220 Background: OMP-59R5, a fully human IgG2 antibody, inhibits signaling of Notch2 and 3 receptors. Tumor regression was seen in Notch3 expressing patient-derived pancreatic cancer xenografts when OMP-59R5 was combined with Nab-P+Gem. The maximum tolerated dose (MTD) of single agent OMP-59R5 was 7.5 mg/kg every other week (Smith, EORTC 2012); the main dose limiting toxicity (DLT) was grade 3 diarrhea. This study is to determine the MTD, pharmacokinetics (PK), pharmacodynamics (PD), and preliminary efficacy of OMP-59R5 in combination with Nab-P+Gem in mPC. Methods: Cohorts of 3 to 6 pts were treated at each dose level of OMP-59R5. OMP-59R5 is given intravenously every other week (days 1 and 15) with GEM 1,000 mg/m2 alone (first two cohorts) or nab-P 125 mg/m2 and GEM 1,000 mg/m2 on days 1, 8, and 15 of every 28-day cycle. Results: By August 30, 2013, 24 pts were treated. No DLTs have occurred. Frequently reported (≥10%) OMP-59R5 treatment-related adverse events (AEs) were: diarrhea (44%), fatigue (44%), and nausea (16%); most were grade 1 or 2 and managed with supportive care. Frequent chemo-related AEs (≥10%) were cytopenia, fatigue, diarrhea, and nausea. GEM or Nab-P+Gem did not alter PK of OMP-59R5. See table for additional data. Conclusions: OMP-59R5 with Nab-P+ Gem is well tolerated. The MTD has not been reached. Encouraging anti-tumor activity is observed. Updated Safety, PK/PD, and efficacy data will be presented. Clinical trial information: NCT01647828. [Table: see text]
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Volokhina, Elena B., Frank Beckers, Jan Tommassen, and Martine P. Bos. "The β-Barrel Outer Membrane Protein Assembly Complex of Neisseria meningitidis." Journal of Bacteriology 191, no. 22 (September 18, 2009): 7074–85. http://dx.doi.org/10.1128/jb.00737-09.
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ABSTRACT The evolutionarily conserved protein Omp85 is required for outer membrane protein (OMP) assembly in gram-negative bacteria and in mitochondria. Its Escherichia coli homolog, designated BamA, functions with four accessory lipoproteins, BamB, BamC, BamD, and BamE, together forming the β-barrel assembly machinery (Bam). Here, we addressed the composition of this machinery and the function of its components in Neisseria meningitidis, a model organism for outer membrane biogenesis studies. Analysis of genome sequences revealed homologs of BamC, BamD (previously described as ComL), and BamE and a second BamE homolog, Mlp. No homolog of BamB was found. As in E. coli, ComL/BamD appeared essential for viability and for OMP assembly, and it could not be replaced by its E. coli homolog. BamE was not essential but was found to contribute to the efficiency of OMP assembly and to the maintenance of OM integrity. A bamC mutant showed only marginal OMP assembly defects, but the impossibility of creating a bamC bamE double mutant further indicated the function of BamC in OMP assembly. An mlp mutant was unaffected in OMP assembly. The results of copurification assays demonstrated the association of BamC, ComL, and BamE with Omp85. Semi-native gel electrophoresis identified the RmpM protein as an additional component of the Omp85 complex, which was confirmed in copurification assays. RmpM was not required for OMP folding but stabilized OMP complexes. Thus, the Bam complex in N. meningitidis consists of Omp85/BamA plus RmpM, BamC, ComL/BamD, and BamE, of which ComL/BamD and BamE appear to be the most important accessory components for OMP assembly.
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SINGH, YOGESH PREET, AMIT KUMAR SINGH, AMITA AGGARWAL, and RAMNATH MISRA. "Evidence of Cellular Immune Response to Outer Membrane Protein ofSalmonella typhimuriumin Patients with Enthesitis-related Arthritis Subtype of Juvenile Idiopathic Arthritis." Journal of Rheumatology 38, no. 1 (November 1, 2010): 161–66. http://dx.doi.org/10.3899/jrheum.100542.
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Objective.Enthesitis-related arthritis subtype of juvenile idiopathic arthritis (JIA-ERA) clinically resembles reactive arthritis (ReA). In patients with ReA the immune response is targeted at the outer membrane protein (OMP) ofSalmonella typhimurium. We studied the immune response in JIA-ERA toS. typhimuriumOMP.Methods.Synovial fluid mononuclear cells (SFMC) and peripheral blood mononuclear cells (PBMC) were isolated from blood and SF of patients with JIA-ERA. Lymphocyte transformation test was done withS. typhimuriumOMP and crude bacterial lysates ofYersinia enterocolitica,Shigella flexneri, andS. typhimurium. IgG and IgA ELISA were performed in serum and SF usingS. typhimuriumOMP as antigen and compared with sera from healthy controls.Results.In PBMC samples (n = 25) an antigen-specific proliferative response was seen in 13 patients and a cross-reactive response in 6. Among these 19 patients, 12 showed response to OMP. In SFMC (n = 15) antigen-specific responses were seen in 3 patients and cross-reactive responses in 9. Among these 12 patients, 11 showed response to OMP. The IgG and IgA anti-OMP antibody concentrations in serum and SF were similar in patients and controls.Conclusion.In JIA-ERA, OMP is the major antigenic target recognized by both SFMC and PBMC. Response to OMP is independent of specific bacterial response, suggesting that OMP is the immunodominant antigen. In these patients, absence of significant humoral response suggests response to OMP is mainly T cell mediated.
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Ashraf, Eslam, Ashraf A. M. Khalaf, and Sara M. Hassan. "Real time FPGA implemnation of SAR radar reconstruction system based on adaptive OMP compressive sensing." Indonesian Journal of Electrical Engineering and Computer Science 20, no. 1 (October 1, 2020): 185. http://dx.doi.org/10.11591/ijeecs.v20.i1.pp185-196.
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<p><span style="font-size: 9pt; font-family: 'Times New Roman', serif;">Synthetic Aperture Radar (SAR) is an imaging system based on the processing of radar echoes. The produced images have a huge amount of data which will be stored onboard or transmitted as a digital signal to the ground station via downlink to be processed. Therefore, some methods of compression on the raw images provides an attractive option for SAR systems design. One of these techniques which used for image reconstruction is the Orthogonal Matching Pursuit (OMP). OMP is an iterative algorithm which need high computational operations. The computational complexity of the iterative algorithms is high due to updating operations of the measurement vector and large number of iterations that are used to reconstruct the images successfully. This paper presents a new adaptive OMP algorithm to overcome this issue by using certain threshold. The new adaptive OMP algorithm is compared with the classical OMP algorithm using the Receiver Operating Characteristic (ROC) curves. The MATLAB simulations show that the new adaptive OMP algorithm improves the probability of detection at lower SNRs, reduce the computational operations as well as the number of required iterations. FPGA implementation of both the classical OMP and the adaptive OMP algorithm are also presented in this paper.</span></p>
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Ong, Marianne Meng-Ann, Mimi Yow, Jestina Tan, and Scott Compton. "Perceived effectiveness of one-minute preceptor in microskills by residents in dental residency training at National Dental Centre Singapore." Proceedings of Singapore Healthcare 26, no. 1 (September 16, 2016): 35–41. http://dx.doi.org/10.1177/2010105816666294.
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Background: The National Dental Centre Singapore (NDCS) has collaborated with the National University of Singapore’s Division of Graduate Dental Studies to provide supervised clinical training in the five dental specialties for residents since the early 1990s. Faculty were first introduced to the one-minute preceptor (OMP) in microskills through in-house workshops held from May to August 2014. Purpose: The aim of this study was to assess if a OMP faculty workshop impacts residents’ perceptions of clinical teaching in NDCS. Methods: First- and second-year residents were asked to rate their perceptions of the quantity and quality of clinical teaching, and the adequacy of the five OMP microskills performed by faculty before and one month after the OMP workshops. Data were analysed using descriptive statistics and non-parametric statistical tests. Results: A total of 37 NDCS clinical faculty participated in the OMP workshops and 34 residents participated in the pre- and post-workshop survey. The short-term impact of the OMP faculty workshop revealed minimal effect on residents’ perceptions of the clinical teaching in both quantity and quality. Conclusion: A one-time OMP workshop for faculty members does not substantially improve residents’ perceptions of the quality or quantity of clinical teaching. The results of this study indicate that the effort to improve clinical teaching by faculty must be a sustained effort. Future studies should investigate the barriers to faculty members’ performance of the OMP microskills.
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Brook, Itzhak, and Alan E. Gober. "Recovery of Potential Pathogens in the Nasopharynx of Healthy and Otitis Media—Prone Children and Their Smoking and Nonsmoking Parents." Annals of Otology, Rhinology & Laryngology 117, no. 10 (October 2008): 727–30. http://dx.doi.org/10.1177/000348940811701003.
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Objectives: Exposure to smoking is associated with colonization with pathogenic bacteria. This study investigated the frequency of isolation of potential pathogens in the nasopharynx of healthy and otitis media–prone (OMP) children and their smoking or nonsmoking parents. Methods: Posterior nasopharynx cultures were taken from 40 healthy and 40 OMP children and one of their parents. Twenty parents in each group were smokers. Potential pathogenic organisms were identified. Results: In the healthy children whose parents smoked, 15 potential pathogens were isolated from the parents and 13 were recovered from their children. Among the healthy children whose parents were nonsmokers, 3 potential pathogens were isolated from 2 parents (p < 0.005, compared to the parents and children in the smoking group) and 7 were recovered from their children. In the OMP children whose parents smoked, 16 potential pathogens were isolated from the parents and 19 were found in their children. Among the OMP children with nonsmoking parents, 3 potential pathogens were isolated from the parents (p < 0.001, compared to the parents and children in the OMP smoking group and the healthy children in the nonsmoking parents group) and 17 were recovered from their children. Conclusions: Parents who smoke are more often colonized with pathogens than those who do not smoke. The nasopharynx of healthy children of smokers harbors a high number of pathogens that are similar to the flora found in their parents and OMP children. Pathogenic organisms were found more often in OMP children of both smoking and nonsmoking parents, as compared to healthy children whose parents were nonsmokers. Concordance with pathogens in the parent was high among the OMP children of smoking parents, but this was not observed in the OMP children of nonsmokers.
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Hernández-Fisac, Inés, Sergio Fernández-Pascual, Henrik Ortsäter, Javier Pizarro-Delgado, Rafael Martín Del Río, Peter Bergsten, and Jorge Tamarit-Rodriguez. "Oxo-4-methylpentanoic acid directs the metabolism of GABA into the Krebs cycle in rat pancreatic islets." Biochemical Journal 400, no. 1 (October 27, 2006): 81–89. http://dx.doi.org/10.1042/bj20060173.
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OMP (oxo-4-methylpentanoic acid) stimulates by itself a biphasic secretion of insulin whereas L-leucine requires the presence of L-glutamine. L-Glutamine is predominantly converted into GABA (γ-aminobutyric acid) in rat islets and L-leucine seems to promote its metabolism in the ‘GABA shunt’ [Fernández-Pascual, Mukala-Nsengu-Tshibangu, Martín del Río and Tamarit-Rodríguez (2004) Biochem. J. 379, 721–729]. In the present study, we have investigated how 10 mM OMP affects L-glutamine metabolism to uncover possible differences with L-leucine that might help to elucidate whether they share a common mechanism of stimulation of insulin secretion. In contrast with L-leucine, OMP alone stimulated a biphasic insulin secretion in rat perifused islets and decreased the islet content of GABA without modifying its extracellular release irrespective of the concentration of L-glutamine in the medium. GABA was transaminated to L-leucine whose intracellular concentration did not change because it was efficiently transported out of the islet cells. The L-[U-14C]-Glutamine (at 0.5 and 10.0 mM) conversion to 14CO2 was enhanced by 10 mM OMP within 30% and 70% respectively. Gabaculine (250 μM), a GABA transaminase inhibitor, suppressed OMP-induced oxygen consumption but not L-leucine- or glucose-stimulated respiration. It also suppressed the OMP-induced decrease in islet GABA content and the OMP-induced increase in insulin release. These results support the view that OMP promotes islet metabolism in the ‘GABA shunt’ generating 2-oxo-glutarate, in the branched-chain α-amino acid transaminase reaction, which would in turn trigger GABA deamination by GABA transaminase. OMP, but not L-leucine, suppressed islet semialdehyde succinic acid reductase activity and this might shift the metabolic flux of the ‘GABA shunt’ from γ-hydroxybutyrate to succinic acid production.
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Murphy, Timothy F., Charmaine Kirkham, Dai-Fang Liu, and Sanjay Sethi. "Human Immune Response to Outer Membrane Protein CD of Moraxella catarrhalis in Adults with Chronic Obstructive Pulmonary Disease." Infection and Immunity 71, no. 3 (March 2003): 1288–94. http://dx.doi.org/10.1128/iai.71.3.1288-1294.2003.
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ABSTRACT Moraxella catarrhalis is a common cause of lower respiratory tract infection in adults with chronic obstructive pulmonary disease (COPD). The antibody response to outer membrane protein (OMP) CD, a highly conserved surface protein of M. catarrhalis under consideration as a vaccine antigen, was studied in adults with COPD following 40 episodes of infection or colonization. Following infection or colonization, 9 of 40 patients developed new serum immunoglobulin G (IgG) to OMP CD, as measured by enzyme-linked immunosorbent assay. Adsorption assays revealed that a proportion of the serum IgG was directed toward surface-exposed epitopes on OMP CD in six of the nine patients who developed new IgG to OMP CD. Immunoblot assays with fusion peptide constructs indicated that the new antibodies that developed after infection or colonization recognized conformational epitopes, particularly in the carboxy region of the protein. Three of 28 patients developed new mucosal IgA to OMP CD in sputum supernatants. This study establishes that OMP CD is a target of a systemic and mucosal immune response following infection and colonization in some patients with COPD.
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Han, Yi, Sankar Renu, Veerupaxagouda Patil, Jennifer Schrock, Ninoshkaly Feliciano-Ruiz, Ramesh Selvaraj, and Gourapura J. Renukaradhya. "Mannose-Modified Chitosan-Nanoparticle-Based Salmonella Subunit OralVaccine-Induced Immune Response and Efficacy in a Challenge Trial in Broilers." Vaccines 8, no. 2 (June 11, 2020): 299. http://dx.doi.org/10.3390/vaccines8020299.
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Controlling Salmonella enterica serovar Enteritidis (SE) infection in broilers is a huge challenge. In this study, our objective was to improve the efficacy of a chitosan nanoparticle (CS)-based Salmonella subunit vaccine for SE, containing immunogenic outer membrane proteins (OMP) and flagellin (FLA), called the CS(OMP+FLA) vaccine, by surface conjugating it with mannose to target dendritic cells, and comparing the immune responses and efficacy with a commercial live Salmonella vaccine in broilers. The CS(OMP+FLA)-based vaccines were administered orally at age 3 days and as a booster dose after three weeks, and the broilers were challenged with SE at 5 weeks of age. Birds were sacrificed 10 days post-challenge and it was observed that CS(OMP+FLA) vaccine surface conjugated with both mannose and FLA produced the greatest SE reduction, by over 1 log10 colony forming unit per gram of the cecal content, which was comparable to a commercial live vaccine. Immunologically, specific mucosal antibody responses were enhanced by FLA-surface-coated CS(OMP+FLA) vaccine, and mannose-bound CS(OMP+FLA) improved the cellular immune response. In addition, increased mRNA expression of Toll-like receptors and cytokine was observed in CS(OMP+FLA)-based-vaccinated birds. The commercial live vaccine failed to induce any such substantial immune response, except that they had a slightly improved T helper cell frequency. Our data suggest that FLA-coated and mannose-modified CS(OMP+FLA) vaccine induced robust innate and adaptive cell-mediated immune responses and substantially reduced the Salmonella load in the intestines of broilers.
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XU, ZHIQIANG. "REMARKS ABOUT ORTHOGONAL MATCHING PURSUIT ALGORITHMS." Advances in Adaptive Data Analysis 04, no. 04 (October 2012): 1250026. http://dx.doi.org/10.1142/s1793536912500264.
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The orthogonal matching pursuit (OMP) is a popular decoder to recover sparse signal in compressed sensing. Our aim is to investigate the theoretical properties of OMP. In particular, we show that the OMP decoder can give (p, q) instance optimality for a large class of encoders with 1 ≤ p ≤ q ≤ 2 and (p, q) ≠ (2, 2).
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Li, Guo Zhu, De Qiang Wang, Zi Kai Zhang, and Zhi Yong Li. "A Weighted OMP Algorithm for Compressive UWB Channel Estimation." Applied Mechanics and Materials 392 (September 2013): 852–56. http://dx.doi.org/10.4028/www.scientific.net/amm.392.852.
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We investigate ultra-wideband (UWB) channel estimation based on compressive sampling (CS), where the orthogonal matching pursuit (OMP) algorithm is employed to recover the channel waveform from noisy measurements. In order to boost the robustness of OMP in the presence of additive Gaussian noise (AWGN), we propose a weighted OMP (WOMP) algorithm. For a given sparse dictionary, weighting factors are assigned to the atoms and a weighted matching process is performed by WOMP. Simulation results show that the proposed WOMP is more robust than the original OMP and can be used to gain better channel estimation precision.
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Wang, Han, Bo Liu, Pingping Sun, and Zhiqiang Ma. "A Topology Structure Based Outer Membrane Proteins Segment Alignment Method." Mathematical Problems in Engineering 2013 (2013): 1–10. http://dx.doi.org/10.1155/2013/541359.
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Outer membrane proteins (OMPs) are transmembrane proteins (TMPs) located in outer membranes. These proteins perform diverse biochemical functions and have immediate medical relevance, so that their spatial structures are important for studying. But the special physicochemical properties of OMP make it hard to obtain their structures experimentally. For the purpose of predicting OMP structures, discriminating OMPs and aligning their sequences to native structures are indispensable steps. We developed a novel method OMSA (Outer Membrane Segment Alignment), which implemented both steps in one program. OMSA integrates OMP-specific topology features to implement a sequence-to-structure alignment, for example, segment type and segment orientation, while a segment-dependent gap penalty model is employed to improve the alignment. Compared to peer top-leading methods, OMSA achieved higher accuracy in both OMP discrimination and alignment, which may further improve OMP structure studying.
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Costello, Shawn M., Ashlee M. Plummer, Patrick J. Fleming, and Karen G. Fleming. "Dynamic periplasmic chaperone reservoir facilitates biogenesis of outer membrane proteins." Proceedings of the National Academy of Sciences 113, no. 33 (August 1, 2016): E4794—E4800. http://dx.doi.org/10.1073/pnas.1601002113.
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Outer membrane protein (OMP) biogenesis is critical to bacterial physiology because the cellular envelope is vital to bacterial pathogenesis and antibiotic resistance. The process of OMP biogenesis has been studied in vivo, and each of its components has been studied in isolation in vitro. This work integrates parameters and observations from both in vivo and in vitro experiments into a holistic computational model termed “Outer Membrane Protein Biogenesis Model” (OMPBioM). We use OMPBioM to assess OMP biogenesis mathematically in a global manner. Using deterministic and stochastic methods, we are able to simulate OMP biogenesis under varying genetic conditions, each of which successfully replicates experimental observations. We observe that OMPs have a prolonged lifetime in the periplasm where an unfolded OMP makes, on average, hundreds of short-lived interactions with chaperones before folding into its native state. We find that some periplasmic chaperones function primarily as quality-control factors; this function complements the folding catalysis function of other chaperones. Additionally, the effective rate for the β-barrel assembly machinery complex necessary for physiological folding was found to be higher than has currently been observed in vitro. Overall, we find a finely tuned balance between thermodynamic and kinetic parameters maximizes OMP folding flux and minimizes aggregation and unnecessary degradation. In sum, OMPBioM provides a global view of OMP biogenesis that yields unique insights into this essential pathway.
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Luke, Nicole R., Thomas A. Russo, Neal Luther, and Anthony A. Campagnari. "Use of an Isogenic Mutant Constructed inMoraxella catarrhalis To Identify a Protective Epitope of Outer Membrane Protein B1 Defined by Monoclonal Antibody 11C6." Infection and Immunity 67, no. 2 (February 1, 1999): 681–87. http://dx.doi.org/10.1128/iai.67.2.681-687.1999.
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ABSTRACT Moraxella catarrhalis-induced otitis media continues to be a significant cause of infection in young children, prompting increased efforts at identifying effective vaccine antigens. We have previously demonstrated that M. catarrhalis expresses specific outer membrane proteins (OMPs) in response to iron limitation and that this organism can utilize transferrin and lactoferrin for in vitro growth. One of these proteins, which binds human transferrin, is OMP B1. As the human host presents a naturally iron-limited environment, proteins, like OMP B1, which are expressed in response to this nutritional stress are potential vaccine antigens. In this study, we have developed monoclonal antibody (MAb) 11C6, which reacts to a surface-exposed epitope of OMP B1 expressed by M. catarrhalis 7169. This antibody was used to cloneompB1, and sequence analysis suggested that OMP B1 is theM. catarrhalis homologue to the transferrin binding protein B described for pathogenic Neisseriaceae, Haemophilus influenzae, Actinobacillus pleuropneumoniae, andM. catarrhalis. Expression of recombinant OMP B1 on the surface of Escherichia coli confers transferrin binding activity, confirming that this protein is likely involved in iron acquisition. In addition, ompB1 was used to construct an isogenic mutant in M. catarrhalis 7169. This mutant, termed 7169b12, was used as the control in bactericidal assays designed to determine if OMP B1 elicits protective antibodies. In the presence of MAb 11C6 and human complement, wild-type 7169 demonstrated a 99% decline in viability, whereas the ompB1 isogenic mutant was resistant to this bactericidal activity. Further analysis with MAb 11C6 revealed the presence of this OMP B1 epitope on 31% of the clinical isolates tested. These data suggest that OMP B1 is a potential vaccine antigen against M. catarrhalis infections.
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Momoi, Fumiki, Tomomi Hashizume, Tomoko Kurita-Ochiai, Yoshikazu Yuki, Hiroshi Kiyono, and Masafumi Yamamoto. "Nasal Vaccination with the 40-Kilodalton Outer Membrane Protein of Porphyromonas gingivalis and a Nontoxic Chimeric Enterotoxin Adjuvant Induces Long-Term Protective Immunity with Reduced Levels of Immunoglobulin E Antibodies." Infection and Immunity 76, no. 6 (April 14, 2008): 2777–84. http://dx.doi.org/10.1128/iai.01502-07.
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ABSTRACT In this study, we demonstrated that the 40-kDa outer membrane protein of Porphyromonas gingivalis (40-kDa OMP) nasally administered with a nontoxic chimeric adjuvant that combines the A subunit of mutant cholera toxin E112K with the pentameric B subunit of heat-labile enterotoxin from enterotoxigenic Escherichia coli (mCTA/LTB) elicited a long-term protective immune response. Immunization with the 40-kDa OMP and mCTA/LTB induced high levels of 40-kDa-OMP-specific immunoglobulin G (IgG) and IgA antibodies (Abs) in sera and elicited a significant IgA anti-40-kDa OMP Ab response in saliva. These Ab responses were maintained for at least 1 year after the immunization. Although using adjuvant mCTA/LTB gave Ab responses in the saliva comparable to those obtained using native cholera toxin (nCT) as the adjuvant, the levels of total IgE and 40-kDa-OMP-specific IgE Abs as well as interleukin-4 levels induced by the immunization with mCTA/LTB were lower than those induced by the immunization with nCT. Importantly, IgG Abs generated by nasal immunization with the 40-kDa OMP plus mCTA/LTB inhibited the coaggregation and hemagglutinin activities of P. gingivalis. Furthermore, the mice given nasal 40-kDa OMP plus mCTA/LTB showed a significant reduction of alveolar bone loss caused by oral infection with P. gingivalis even 1 year after the immunization compared to the loss in unimmunized mice. Because mCTA/LTB is nontoxic, nasally administered 40-kDa OMP together with mCTA/LTB should be an effective and safe mucosal vaccine against P. gingivalis infection in humans and may be an important tool for the prevention of chronic periodontitis.
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Nandi, Bisweswar, Kathryn Hogle, Nicholas Vitko, and Gary M. Winslow. "CD4 T-Cell Epitopes Associated with Protective Immunity Induced following Vaccination of Mice with an Ehrlichial Variable Outer Membrane Protein." Infection and Immunity 75, no. 11 (August 13, 2007): 5453–59. http://dx.doi.org/10.1128/iai.00713-07.
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ABSTRACT The ehrlichiae express variable outer membrane proteins (OMPs) that play important roles in both pathogenesis and host defense. Previous studies revealed that OMPs are immunodominant B-cell antigens and that passive transfer of anti-OMP antibodies can protect SCID mice from fatal ehrlichial infection. In this study, we used a model of fatal monocytotropic ehrlichiosis caused by Ehrlichia bacteria from Ixodes ovatus (IOE) to determine whether OMP immunization could generate protective immunity in immunocompetent mice. Immunization of C57BL/6 mice with a purified recombinant OMP expressed by IOE omp19 generated protection from fatal IOE infection and elicited robust humoral and CD4 T-cell responses. To identify CD4 T-cell epitopes within OMPs, we performed enzyme-linked immunospot analyses for gamma interferon (IFN-γ) production using a panel of overlapping 16-mer peptides from IOE OMP-19. Five immunoreactive peptides comprising residues 30 to 45, 77 to 92, 107 to 122, 197 to 212, and 247 to 264 were identified; the strongest response was generated against OMP-19107-122. Most of the peptides are conserved between E. muris and E. chaffeensis OMP-19, and they elicited IFN-γ production in CD4 T cells from E. muris-infected mice, indicating that T-cell epitope cross-reactivity likely contributes to heterologous immunity. Accordingly, CD4 T-cell responses to both OMP-19 and OMP-19107-122 were of greater magnitude following high-dose IOE challenge of mice that had been immunized by prior infection with E. muris. Our studies cumulatively identify B- and T-cell epitopes that are associated with protective homologous and heterologous immunity during ehrlichial infection.
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Murphy, Timothy F., Aimee L. Brauer, Norine Yuskiw, and Thomas J. Hiltke. "Antigenic Structure of Outer Membrane Protein E ofMoraxella catarrhalis and Construction and Characterization of Mutants." Infection and Immunity 68, no. 11 (November 1, 2000): 6250–56. http://dx.doi.org/10.1128/iai.68.11.6250-6256.2000.
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ABSTRACT Outer membrane protein E (OMP E) is a 50-kDa protein ofMoraxella catarrhalis which possesses several characteristics indicating that the protein will be an effective vaccine antigen. To study the antigenic structure of OMP E, eight monoclonal antibodies were developed and characterized. Three of the antibodies recognized epitopes which are present on the bacterial surface. Fusion peptides corresponding to overlapping regions of OMP E were constructed, and immunoblot assays were performed to localize the areas of the molecule bound by the monoclonal antibodies. These studies identified a surface-exposed epitope in the region of amino acids 80 through 180. To further study the protein, two mutants which lack OMP E were constructed. In bactericidal assays, the mutants were more readily killed by normal human serum compared to the isogenic parent strains. These results indicate that OMP E is involved in the expression of serum resistance of M. catarrhalis.
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Makeyeva, Yuliya, Christopher Nicol, William L. Ledger, and David K. Ryugo. "Immunocytochemical Localization of Olfactory-signaling Molecules in Human and Rat Spermatozoa." Journal of Histochemistry & Cytochemistry 68, no. 7 (June 30, 2020): 491–513. http://dx.doi.org/10.1369/0022155420939833.
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Expression of olfactory receptors (ORs) in non-olfactory tissues has been widely reported over the last 20 years. Olfactory marker protein (OMP) is highly expressed in mature olfactory sensory neurons (mOSNs) of the olfactory epithelium. It is involved in the olfactory signal transduction pathway, which is mediated by well-conserved components, including ORs, olfactory G protein (Golf), and adenylyl cyclase 3 (AC3). OMP is widely expressed in non-olfactory tissues with an apparent preference for motile cells. We hypothesized that OMP is expressed in compartment-specific locations and co-localize with an OR, Golf, and AC3 in rat epididymal and human-ejaculated spermatozoa. We used immunocytochemistry to examine the expression patterns of OMP and OR6B2 (human OR, served as positive olfactory control) in experimentally induced modes of activation and determine whether there are any observable differences in proteins expression during the post-ejaculatory stages of spermatozoal functional maturation. We found that OMP was expressed in compartment-specific locations in human and rat spermatozoa. OMP was co-expressed with Golf and AC3 in rat spermatozoa and with OR6B2 in all three modes of activation (control, activated, and hyperactivated), and the mode of activation changed the co-expression pattern in acrosomal-reacted human spermatozoa. These observations suggest that OMP expression is a reliable indicator of OR-mediated chemoreception, may be used to identify ectopically expressed ORs, and could participate in second messenger signaling cascades that mediate fertility.
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Wang, Zhizhan, Yuzhou Li, Chengcai Wang, Donghong Ouyang, and Yunlong Huang. "A-OMP: An Adaptive OMP Algorithm for Underwater Acoustic OFDM Channel Estimation." IEEE Wireless Communications Letters 10, no. 8 (August 2021): 1761–65. http://dx.doi.org/10.1109/lwc.2021.3079225.
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Carillo, Petronia, Pasqualina Woodrow, Giampaolo Raimondi, Christophe El-Nakhel, Antonio Pannico, Marios C. Kyriacou, Giuseppe Colla, et al. "Omeprazole Promotes Chloride Exclusion and Induces Salt Tolerance in Greenhouse Basil." Agronomy 9, no. 7 (July 4, 2019): 355. http://dx.doi.org/10.3390/agronomy9070355.
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The role of small bioactive molecules (<500 Da) in mechanisms improving resource use efficiency in plants under stress conditions draws increasing interest. One such molecule is omeprazole (OMP), a benzimidazole derivative and inhibitor of animal proton pumps shown to improve nitrate uptake and exclusion of toxic ions, especially of chloride from the cytosol of salt-stressed leaves. Currently, OMP was applied as substrate drench at two rates (0 or 10 μM) on hydroponic basil (Ocimum basilicum L. cv. Genovese) grown under decreasing NO3−:Cl− ratio (80:20, 60:40, 40:60, or 20:80). Chloride concentration and stomatal resistance increased while transpiration, net CO2 assimilation rate and beneficial ions (NO3−, PO43−, and SO42−) decreased with reduced NO3−:Cl− ratio under the 0 μM OMP treatment. The negative effects of chloride were not only mitigated by the 10 μM OMP application in all treatments, with the exception of 20:80 NO3−:Cl−, but plant growth at 80:20, 60:40, and 40:60 NO3−:Cl− ratios receiving OMP application showed maximum fresh yield (+13%, 24%, and 22%, respectively), shoot (+10%, 25%, and 21%, respectively) and root (+32%, 76%, and 75%, respectively) biomass compared to the corresponding untreated treatments. OMP was not directly involved in ion homeostasis and compartmentalization of vacuolar or apoplastic chloride. However, it was active in limiting chloride loading into the shoot, as manifested by the lower chloride concentration in the 80:20, 60:40, and 40:60 NO3−:Cl− treatments compared to the respective controls (−41%, −37%, and −24%), favoring instead that of nitrate and potassium while also boosting photosynthetic activity. Despite its unequivocally beneficial effect on plants, the large-scale application of OMP is currently limited by the molecule’s high cost. However, further studies are warranted to unravel the molecular mechanisms of OMP-induced reduction of chloride loading to shoot and improved salt tolerance.
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Sardesai, Sagar D., Alexandra Thomas, Christopher Gallagher, Filipa Lynce, Yvonne Lynn Ottaviano, Tarah Jean Ballinger, Bryan P. Schneider, et al. "Inhibiting fatty acid synthase in operable triple negative breast cancer." Journal of Clinical Oncology 38, no. 15_suppl (May 20, 2020): 584. http://dx.doi.org/10.1200/jco.2020.38.15_suppl.584.
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584 Background: Fatty acid synthase (FASN) is overexpressed in 70% of newly diagnosed triple negative breast cancer (TNBC) and is associated with poor prognosis. In vitro, FASN overexpression induces drug resistance to DNA damaging agents. Proton pump inhibitors (PPI) selectively inhibit FASN activity and induce apoptosis in breast cancer cell lines with minimal effect on non-malignant cells. We report the results of a single arm phase II study of high dose omeprazole (OMP) in combination with anthracycline- taxane (AC-T) based neoadjuvant chemotherapy. Methods: Patients (pts) with operable TNBC independent of baseline FASN expression; and no prior PPI use within 12 months were enrolled. Pts began OMP 80 mg PO BID for 4-7 days prior to AC-T; carboplatin was allowed per physician discretion. OMP was continued until surgery. Paired biopsy samples were obtained before and after OMP monotherapy. The primary endpoint was pathologic complete response (pCR), defined as no residual invasive disease in breast or axilla, in pts with baseline FASN expression (FASN+) assessed using immunohistochemistry. Relevant secondary endpoints included pCR in the intent to treat population, change in FASN expression, enzyme activity and downstream target gene expression after OMP monotherapy; safety and limited OMP pharmacokinetics. We targeted a pCR rate of 60% in FASN+ pts (null pCR ~ 40%) with 80% power and alpha of 0.10. Results: A total of 42 pts were recruited from 5 US sites. Median age was 51y (28-72). Most pts had >cT2 (33, 79%) and ≥N1 (22, 52%) disease. 14 (33%) were African American. FASN expression prior to AC-T was identified in 28 (85%) samples available for analysis. The pCR rate was 71.4% (95% CI 51.3 to 86.8) in FASN+ pts and 71.8 %( 95% CI 55.1 to 85.0) in all enrolled pts. Fifteen pts (36%) received carboplatin with AC-T; pCR in this subset was 73%. Peak OMP concentration was significantly higher than IC50 observed during preclinical testing; FASN positivity significantly decreased with OMP monotherapy from 0.53(SD 0.25) at baseline to 0.38(SD 0.30; p = 0.02). OMP was well tolerated with no known grade (G) 3 or 4 toxicities. Chemotherapy toxicity was similar to prior studies using AC-T with G3 or 4 neutropenia (19%), febrile neutropenia (7%) and peripheral neuropathy (7%) being the most common. Conclusions: Consistent with previous studies, FASN is frequently expressed in early stage TNBC. OMP can be safely administered in doses that inhibit FASN. The addition of high dose OMP to neoadjuvant AC-T yields a promising pCR rate without adding toxicity. Funded by the Breast Cancer Research Foundation. Clinical trial information: NCT02595372 .
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Ahmadi, Hojat, Bahman Tabaraie, Shayan Maleknia, Farahnaz Pormirzagholi, Mehdi Nejati, and Mohammad Hosein Hedayati. "Immunological evaluation of OMP-F of native Iranian Pseudomonas aeruginosa as a protective vaccine." Journal of Infection in Developing Countries 6, no. 10 (October 19, 2012): 721–26. http://dx.doi.org/10.3855/jidc.2220.
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Introduction: This study involved 300 Pseudomonas aeruginosa strains isolated from patients admitted in four Tehran hospitals. Using standard O-specific typing sera, they were all grouped into 16 strains out of 17 known P. aeruginosa. The strains were lyophilized and each was given a code according to the Collection of Standard Bacteria, Pasteur Institute of Iran (CSBPI) for further investigations. Methodology: Among all clinical samples, CSBPI: 16-190 was the most prevalent P. aeruginosa serotype which showed a high agglutination titer (4+, 320) against homologous O-specific typing sera. This serotype was selected for extraction of P. aeruginosa major outer membrane vesicles (OMP-F). OMP-F vesicles were extracted and purified according to the Deoxycholate Ultracentrifuge Differentiation Technique. Purity and molecular weight of OMP-F were determined by SDS-PAGE and the ability of OMP-F vesicles to induce high titers of antibody in rabbit, which was shown as a sharp antibody-antigen precipitation line in the agarose gel immune-diffusion technique. Results: Passive immunization of mice with anti-rabbit OMP-F antisera induced a high level of protection when the mice were post-challenged with 2×LD50 of live P. aeruginosa CSBPI: 16-190. Furthermore, active immunization of mice with 50 µg of OMP-F could protect mice against 2×LD50 of live homologous (100% protection) and 15 heterologous native Iranian P. aeruginosa serotypes with 50-100% level of protection. Conclusions: These investigations indicate that purified OMP-F of CSBPI: 16-190 can be regarded as a safe protective immunogen in vaccinothrapy against all P. aeruginosaimmunotype isolated in Iran.
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Ning, Fangli, Yong Liu, Chao Zhang, Jingang Wei, Xudong Shi, and Juan Wei. "Acoustic Imaging with Compressed Sensing and Microphone Arrays." Journal of Computational Acoustics 25, no. 04 (November 21, 2017): 1750027. http://dx.doi.org/10.1142/s0218396x17500278.
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This work studies the acoustic imaging problem with compressed sensing (CS) and microphone arrays. The CS algorithm with Basis Pursuit (BP) algorithm has shown satisfying results in acoustic imaging, the maps of which are characterized by super-resolution. However, the performance of the CS algorithm with the BP algorithm is limited to Restricted Isometry Property (RIP), and the algorithm has a long CPU-time. We propose a new CS algorithm with Orthogonal Matching Pursuit (OMP) algorithm for acoustic imaging. The performance of the OMP algorithm with regard to RIP is examined through numerical simulation in this work. The simulation results and CPU-time for OMP algorithm are compared with those of the BP algorithm and the conventional beamformer (CBF). When the RIP does not hold, satisfying results can still be obtained by the OMP algorithm, and the CPU-time for OMP algorithm is far less than BP algorithm. In order to validate the feasibility of the OMP algorithm in acoustic imaging, an experiment is also conducted in a semi-anechoic room. Two mobile phones are served as sound sources. We investigate the mobile phones sources and compare the experimental results with those of BP algorithm and CBF method. The OMP algorithm can locate the main sources at low frequencies, while the CBF method can just give a rough indication and fails for low frequencies due to the width of its main lobe. Due to many reconstructed sources outside of the expected source positions existing on the map, the BP algorithm fails to locate the main sources at low frequencies.
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Kalenski, Julia, Elina Mancina, Pascal Paschenda, Christian Beckers, Christian Bleilevens, Ľubomíra Tóthová, Peter Boor, Dominik Gross, René H. Tolba, and Benedict M. Doorschodt. "Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys." European Surgical Research 57, no. 1-2 (2016): 10–21. http://dx.doi.org/10.1159/000444851.
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Background/Aim: The global shortage of donor organs for transplantation has necessitated the expansion of the organ pool through increased use of organs from less ideal donors. Venous systemic oxygen persufflation (VSOP) and oxygenated machine perfusion (OMP) have previously demonstrated beneficial results compared to cold storage (CS) in the preservation of warm-ischemia-damaged kidney grafts. The aim of this study was to compare the efficacy of VSOP and OMP for the preservation of warm-ischemia-damaged porcine kidneys using the recently introduced Ecosol preservation solution compared to CS using Ecosol or histidine-tryptophan-ketoglutarate solution (HTK). Materials and Methods: Kidneys from German Landrace pigs (n = 5/group) were retrieved and washed out with either Ecosol or HTK after 45 min of clamping of the renal pedicle. As controls, kidneys without warm ischemia, cold stored for 24 h in HTK, were employed. Following 24 h of preservation by VSOP, OMP, CS-Ecosol, or CS-HTK, renal function and damage were assessed during 1 h using the isolated perfused porcine kidney model. Results: During reperfusion, urine production was significantly higher in the VSOP and OMP groups than in the CS-HTK group; however, only VSOP could demonstrate lower urine protein concentrations and fractional excretion of sodium, which did not differ from the non-warm-ischemia-damaged control group. VSOP, CS-Ecosol, and controls showed better maintenance of the acid-base balance than CS-HTK. Reduced lipid peroxidation, as reflected in postreperfusion tissue thiobarbituric acid-reactive substance levels, was observed in the VSOP group compared to the OMP group, and the VSOP and CS-Ecosol groups had concentrations similar to the controls. The ratio of reduced to oxidized glutathione was higher in the VSOP, OMP, and CS-Ecosol groups than in the CS-HTK group and controls, with a higher ratio in the VSOP than in the OMP group. Conclusion: VSOP was associated with mitigation of oxidative stress in comparison to OMP and CS. Preservation of warm-ischemia-damaged porcine kidneys by VSOP was improved compared to OMP and CS, and was comparable to preservation of non-warm-ischemia-damaged cold-stored kidneys.
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Kumagai, Yumi, Haibin Huang, and Yasuko Rikihisa. "Expression and Porin Activity of P28 and OMP-1F during Intracellular Ehrlichia chaffeensis Development." Journal of Bacteriology 190, no. 10 (March 21, 2008): 3597–605. http://dx.doi.org/10.1128/jb.02017-07.
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ABSTRACT Ehrlichia chaffeensis, an obligatory intracellular gram-negative bacterium, must take up various nutrients and metabolic compounds because it lacks many genes involved in metabolism. Nutrient uptake by a gram-negative bacterium occurs primarily through pores or channels in the bacterial outer membrane. Here we demonstrate that isolated E. chaffeensis outer membranes have porin activities, as determined by a proteoliposome swelling assay. The activity was partially blocked by an antibody that recognizes the two most abundant outer membrane proteins, P28/OMP-19 and OMP-1F/OMP-18. Both proteins were predicted to have structural features characteristic of porins, including 12 transmembrane segments comprised of amphipathic and antiparallel β-strands. The sodium dodecyl sulfate stability of the two proteins was consistent with a β-barrel structure. Isolated native P28 and OMP-1F exhibited porin activities, with pore sizes similar to and larger than, respectively, that of OprF, which is the porin with the largest pore size known to date. E. chaffeensis experiences temperature changes during transmission by ticks. During the intracellular development of E. chaffeensis, both P28 and OMP-1F were expressed mostly in the mid-exponential growth phase at 37°C and the late-exponential growth phase at 28°C. The porin activity of proteoliposomes reconstituted with proteins from the outer membrane fractions derived from bacteria in the mid- and late-exponential growth phases at 28°C and 37°C correlated with the expression levels of P28 and OMP-1F. These results imply that P28 and OMP-1F function as porins with large pore sizes, suggesting that the differential expression of these two proteins might regulate nutrient uptake during intracellular E. chaffeensis development at both temperatures.
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Kim, Seonggeon, Uihyun Yun, Jaehyuk Jang, Geunsu Seo, Jongjin Kang, Heung-No Lee, and Minjae Lee. "Reduced Computational Complexity Orthogonal Matching Pursuit Using a Novel Partitioned Inversion Technique for Compressive Sensing." Electronics 7, no. 9 (September 18, 2018): 206. http://dx.doi.org/10.3390/electronics7090206.
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This paper reports a field-programmable gate array (FPGA) design of compressed sensing (CS) using the orthogonal matching pursuit (OMP) algorithm. While solving the least-squares (LS) problem in the OMP algorithm, the complexity of the matrix inversion operation at every loop is reduced by the proposed partitioned inversion that utilizes the inversion result in the previous iteration. By the proposed matrix (n × n) inversion method inside the OMP, the number of operations is reduced down from O(n3) to O(n2). The OMP algorithm is implemented with a Xilinx Kintex UltraScale. The architecture with the proposed partitioned inversion involves 722 less DSP48E compared with the conventional method. It operates with a sample period of 4 ns, signal reconstruction time of 27 μs, and peak signal to noise ratio (PSNR) of 30.26 dB.
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SINGH, AMIT KUMAR, AMITA AGGARWAL, SMRITI CHAURASIA, and RAMNATH MISRA. "Identification of Immunogenic HLA-B*27:05 Binding Peptides of Salmonella Outer Membrane Protein in Patients with Reactive Arthritis and Undifferentiated Spondyloarthropathy." Journal of Rheumatology 40, no. 2 (January 15, 2013): 173–85. http://dx.doi.org/10.3899/jrheum.110849.
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Objective.Salmonella outer membrane proteins (OMP) are major immunogenic targets to synovial fluid lymphocytes of patients with reactive arthritis (ReA)/undifferentiated spondyloarthropathy (uSpA). Because these patients have genetic predisposition to HLA-B*27 and its subtype HLA-B*27:05, we sought to identify immunogenic HLA-B*27:05-binding salmonella OMP peptides in patients with ReA/uSpA.Methods.A total of 125 HLA-B*27:05-binding salmonella OMP peptides identified using ProPred-I software were synthesized and grouped in 23 pools. The peptide pools, along with crude enteric bacterial lysates and salmonella OMP, were cultured with synovial fluid (SF) or peripheral blood mononuclear cells (PBMC) from 23 patients with ReA/uSpA, 10 with rheumatoid arthritis (RA), and 10 healthy individuals in 96-well culture plates. Proliferation was measured by tritiated thymidine uptake and interferon-γ (IFN-γ) levels in culture supernatant. Individual peptides from pools having significant responses were retested with cryopreserved cells. Immunogenic peptides thus identified were further tested in 5 additional new patients with ReA/uSpA by flow cytometry. A Basic Local Alignment Search Tool program was used to search for similar peptides from a protein bank of arthritogenic bacteria and human protein.Results.Nineteen of 23 SFMC from ReA/uSpA showed a significant proliferative response to salmonella OMP, with minimal response of PBMC (1/10) from ReA/uSpA, SFMC from RA (1/10), or PBMC from controls (1/10). Nine salmonella OMP peptides, QRAEMLPTL, SRSGLNIAL, LRFLYAKSL, RLEGTWVKL, ARCIAPYAL, KLFLTTAAL, YRNSDFFGL, QRPAVRVKL, and YRVGPGDVL, were identified. Response to QRAEMLPTL was seen in 6/7 HLA-B*27:05-positive patients. All immunogenic peptides had sequence similarity with peptides from arthritogenic bacterial proteins, while 5 had similarity with peptides from human proteins.ConclusionNine novel immunogenic OMP peptides binding to HLA-B*27:05 were identified that showed sequence similarity with other arthritogenic bacteria.
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Hirano, Takashi, Yoshinori Kadowaki, Takayuki Matsunaga, Kazuhiro Yoshinaga, Toshiaki Kawano, Munehito Moriyama, and Masashi Suzuki. "Interaction Between Regulatory T Cells and Antibody-Producing B Cells for Immune Responses at the Upper Respiratory Mucosa Against Nontypeable Haemophilus influenzae: In Vitro Assay Model." Annals of Otology, Rhinology & Laryngology 128, no. 6_suppl (May 15, 2019): 45S—51S. http://dx.doi.org/10.1177/0003489419837994.
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Objectives: The aim of this study was to investigate the effect of regulatory T cells (Tregs) on B-cell immune responses against outer membrane protein (OMP) from nontypeable Haemophilus influenzae (NTHi) in vitro, to clarify its exact mechanism from an immunologic standpoint. Methods: Mice were vaccinated intranasally with OMP to induce OMP-specific immune responses in the nasal mucosa. Mononuclear cells (MNCs) were collected from the nasal mucosa, and Tregs and helper T (Th) cells were isolated separately from the spleens of those mice. Three different cell culture groups were allocated: MNCs cocultured with Tregs, MNCs cocultured with Th cells, and MNCs cultured alone. At 24 and 72 hours after cell culture, the concentrations of various cytokines and antibodies in culture supernatants were measured to assess the effects of Tregs and Th cells on B-cell responses. Cytokine levels and specific anti-OMP antibody levels in culture media were determined using enzyme-linked immunosorbent assay. CD69 or CD80 expression on B220-positive cells was detected using flow cytometric analysis. Results: Th1 and Th2 cytokine concentrations were significantly elevated in the 3 groups incubated with OMP from 24 to 72 hours. Additionally, interleukin-10 levels were significantly higher in the Treg and Th groups than in the control group. Levels of OMP-specific immunoglobulin A did not differ significantly among the groups. The ratios of CD69+B220+ B2 cells were nearly the same in the 3 groups; however, the ratio of CD80+B220+ B2 cells was higher in the control group than in the Treg and Th groups during incubation. Conclusions: Tregs and Th cells did not affect OMP-specific immunoglobulin A production in this study. However, these cells may partially inhibit B-cell functions, such as T-cell activation. These inhibitory effects may be related to interleukin-10.
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Abassi, Zaid A., Ali Yahia, Samar Zeid, Tony Karram, Eliahu Golomb, Joseph Winaver, and Aaron Hoffman. "Cardiac and renal effects of omapatrilat, a vasopeptidase inhibitor, in rats with experimental congestive heart failure." American Journal of Physiology-Heart and Circulatory Physiology 288, no. 2 (February 2005): H722—H728. http://dx.doi.org/10.1152/ajpheart.00737.2004.
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Omapatrilat (OMP) is a novel mixed inhibitor of angiotensin-converting enzyme (ACE) and neutral endopeptidase 24.11 (NEP), the enzyme that metabolizes natriuretic peptides. Congestive heart failure (CHF) is characterized by excessive sodium retention, attributed to both an excessive effect of angiotensin II and diminished responsiveness to natriuretic peptides. In this study, we examined the acute and chronic renal and cardiac effects of OMP in rats with compensated [urinary sodium excretion (UNaV) > 1,200 μeq/day] and decompensated (UNaV < 100 μeq/day) CHF, induced by a surgical aortocaval fistula (ACF). Bolus injection of OMP (10 mg/kg) to sham controls produced significant diuretic and natriuretic responses [UNaV increased from 0.67 ± 0.19 to 3.27 ± 1.35 μeq/min, P < 0.05; fractional sodium excretion (FENa) increased from 0.23 ± 0.06 to 0.95 ± 0.34%, P < 0.01] despite a significant decline in blood pressure (BP). Rats with compensated CHF displayed blunted diuresis and natriuresis to this dose of OMP but a significant decrease in BP. However, in rats with decompensated CHF, OMP induced significant natriuresis (FENa increased from 0.18 ± 0.15 to 0.82 ± 0.26%, P < 0.05) despite a further decrease in BP (from 90 ± 9 to 71 ± 6 mmHg, P < 0.01). Two weeks after ACF, the heart/body weight ratio was significantly greater in rats with CHF than controls (0.51 ± 0.026 vs. 0.30 ± 0.004%, P < 0.0001), and UNaV was significantly lower. Immediate or late (1 or 6 days after ACF) OMP treatment in the drinking water (140 mg/l) reduced cardiac hypertrophy to 0.41–0.43% ( P < 0.01) and induced natriuresis. These results suggest that OMP improves both sodium balance and cardiac remodeling and might be advantageous to ACE inhibitors for the treatment of decompensated CHF.
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Kremmyda, Olympia, Claudia Frenzel, Katharina Hüfner, Nicolina Goldschagg, Christian Brem, Jennifer Linn, and Michael Strupp. "Acute binocular diplopia: peripheral or central?" Journal of Neurology 267, S1 (August 14, 2020): 136–42. http://dx.doi.org/10.1007/s00415-020-10088-y.
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Abstract Objectives Acute diplopia is a diagnostic challenge for clinicians, in particular in the emergency department. The most common cause of acute diplopia are ocular motor nerve palsies (OMP). In this prospective study, we focused on identifying the most crucial signs and symptoms for differentiating between peripheral and central OMP. Methods We prospectively evaluated 56 non-consecutive patients who presented at our emergency department with acute binocular diplopia (≤ 10 days). The patient history was taken using a standardized questionnaire and patients underwent a neurological, neuro-ophthalmological and neuro-otological examination, including measurement of the subjective visual vertical (SVV), Harms tangent screen test, and cranial MRI. Results Forty-six out of 56 patients were diagnosed with an ocular motor cranial nerve palsy (OMP), 21 of peripheral and 23 of central origin; in two patients, the etiology remained unknown. The following features were different in peripheral and central OMP: (1) the presence of vertigo/dizziness was more frequent in central (43.5%) than in peripheral (9.5%) OMP. (2) Central ocular motor signs, such as saccadic smooth pursuit, additional internuclear ophthalmoplegia, skew deviation, and saccade palsies, were also found more frequently in the central than in the peripheral group (86.7% vs. 33.3%). (3) Further, a pathological SVV deviation by monocular testing of the non-affected eye was also more common in central (77.3%) than in peripheral OMP (38.9%). The presence of all three factors has a positive predictive value of 100% (CI 50–100%) for the presence of a central lesion. Conclusions In acute diplopia due to central OMP, the most important accompanying symptom is vertigo/dizziness, and the most important clinical signs are central ocular motor disorders (which require examination of the non-paretic eye) and an SVV deviation in the non-paretic eye.
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Cheema, Harwinder Kaur, Rajiv Arora, and Rakesh Kumar. "Evaluation of One Minute Preceptor (OMP) as a Teaching Tool for Interns in the Department of Obstetrics & Gynaecology - A Cross-Sectional Study in Punjab Institute of Medical Sciences." Journal of Evidence Based Medicine and Healthcare 8, no. 23 (June 7, 2021): 1970–76. http://dx.doi.org/10.18410/jebmh/2021/370.
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BACKGROUND In Punjab Institute of Medical Sciences, Jalandhar, one minute preceptor (OMP) is the 1st such teaching - learning tool, which has been used in the Department of Obstetrics and Gynaecology in a busy out-patient set up for effective clinical teaching for interns. We wanted to assess the perception of interns for acceptability of one minute preceptor as a teaching tool in Obstetrics & Gynaecology Department. We also wanted to assess the feasibility & acceptability of one minute preceptor as a teaching tool for the faculty in the Department of Obstetrics & Gynaecology in PIMS. METHODS A prospective cross-sectional study was done in Punjab Institute of Medical Sciences, with 30 interns posted on rotational duty in the Department of Obstetrics & Gynaecology. 6 faculty members were sensitized along with interns for teaching one minute preceptor. 3 sessions were conducted with 3 different topics. Thereafter perception of interns & faculty was obtained through validated questionnaire. Data analysis was done by calculating percentage & frequency. Qualitative analysis was done by thematic analysis and word cloud. RESULTS Almost all learners agreed that one minute preceptor (OMP) is better than traditional teaching methods as it helps in building concepts of the subject. 83.3 % interns admitted that OMP brings out the strengths & weakness in them. All learners were of the view that OMP enhances clinical reasoning skills. All members of the faculty agreed that all domains of learning are assessed during OMP and felt that OMP can be used as a teaching tool in busy OPD. CONCLUSIONS One-minute preceptor has been proved to be an effective teaching tool in the busy OPD set up. It helps to teach essential skills to diagnose & manage common Obstetrical & Gynaecological problems in a short span of time. KEYWORDS One-Minute Preceptor (OMP), Obstetrics & Gynaecology (OBG), Teaching Tool, Internship, Out-Patient Department (OPD)
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Lu, Lianming, Baoping Cheng, Jinai Yao, Aitian Peng, Danchao Du, Guocheng Fan, Xiurong Hu, Liping Zhang, and Guoqing Chen. "A New Diagnostic System for Detection of ‘Candidatus Liberibacter asiaticus’ Infection in Citrus." Plant Disease 97, no. 10 (October 2013): 1295–300. http://dx.doi.org/10.1094/pdis-11-12-1086-re.
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In this study, two polyclonal antibodies were produced against the Omp protein of ‘Candidatus Liberibacter asiaticus’. First, omp genes were sequenced to exhibit 99.9% identity among 137 isolates collected from different geographical origins. Then, two peptides containing the hydrophobic polypeptide-transport-associated (POTRA) domain and β-barrel domain, respectively, were identified on Omp protein. After that, these two peptides were overexpressed in Escherichia coli and purified by affinity chromatography to immunize the white rabbits. Finally, the antiserum was purified by affinity chromatography. The two Omp antibodies gave positive results (0.454 to 0.633, 1:1,600 dilution) in enzyme-linked immunosorbent assay against ‘Ca. L. asiaticus’-infected samples collected from different geographical origins but revealed negative results against other pathogen-infected, nutrient-deficient and healthy samples. The antibody against the POTRA domain of Omp protein could detect ‘Ca. L. asiaticus’ in 45.7% of the symptomatic samples compared with a 56.2% detection rate with a polymerase chain reaction assay. These new antibodies will provide a very useful supplement to the current approaches to ‘Ca. L. asiaticus’ detection and also provide powerful research tools for tracking distribution of this pathogen in vivo.
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Mufida, Diana Chusna, Candra Bumi, and Heni Fatmawati. "PERAN PROTEIN MEMBRAN LUAR 55 kDa Salmonella typhi ISOLAT JEMBER SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN." Berkala Penelitian Hayati 15, no. 1 (December 31, 2009): 11–16. http://dx.doi.org/10.23869/bphjbr.15.1.20093.
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Salmonella typhi is an obligate pathogen that is usually found in clinical specimen from typhoid fever patients. The pathogenic mechanism of bacteria are not fully elucidated especially its potential activity of the outer membrane protein (OMP) as hemaglutinin and adhesion molecule. After identification, bacterial isolate of outer membrane protein fraction 12,5% SDS-PAGE were used to isolate OMP followed by hemaglutinin test and in vitro adhesion test. The study showed that the 55 kDa protein of S. typhi is a hemaglutinin protein that could agglutinate, Wistar mouse erythrocytes. The 55 kDa OMP is also an adhesion protein showed by its activity to adherence to Wistar mice’s enterocyt. The increased dose of 55 kDa OMP will decrease the amount of S. typhi bacteria adherenced to Wistar mice’s enterocyt. Outer membrane protein 55 kDa S. typhi as hemagglutinin and also adhesion protein.
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Grau-López, L., A. Teniente-Serra, M. Tintoré, A. Rovira, L. Ramió-Torrenta, L. Brieva, A. Saiz, et al. "Similar biological effect of high-dose oral versus intravenous methylprednisolone in multiple sclerosis relapses." Multiple Sclerosis Journal 21, no. 5 (August 21, 2014): 646–50. http://dx.doi.org/10.1177/1352458514546786.
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Our aim was to investigate differences in immune mechanisms in multiple sclerosis (MS) relapse, after high-dose oral methylprednisolone (oMP) or intravenous methylprednisolone (ivMP). We measured serum cytokines (IL-2, IL-4, IL-6, IL-10, IL-17, TNF-α and IFN-γ) in 39 of 49 MS patients with moderate-severe relapse, whom were treated with ivMP or oMP in a placebo-controlled, non-inferiority clinical trial. We assessed these cytokine levels at baseline and at 1 and 4 weeks post-treatment. The cytokine levels between oMP and ivMP were similar at any time. Proinflammatory cytokines (IL-6 and IFN-γ) were significantly decreased in both groups at week 1 ( p = 0.05 / p = 0.03) and at week 4 ( p = 0.04 / p = 0.05). This study provides further confirmatory evidence that oMP is not inferior to ivMP. Trial registration: clinicaltrials.gov identifier: NCT00753792
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Waseem, Naureen, Khadija Iqbal, Hina Kundi, Irum Rehman, Naveeda Zaigham, and Abdullah Qamar. "Teaching anatomy using OMP in combination with spotting: Re-Shaping the subject." Professional Medical Journal 27, no. 04 (April 10, 2020): 730–36. http://dx.doi.org/10.29309/tpmj/2020.27.04.3460.
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Objectives: The study determines the possible advantages of using the OMP in the gross anatomy laboratory. It focuses on using teaching strategies which focus on the application of knowledge, rather than mere structure identification. The in cooperation of OMP in gross anatomy teaching along with structure identification is the main focus of this study. Study Design: Randomized Control Trial (the spotting methodology & OMP were themselves and intervention for to groups). Setting: Department of Anatomy Islam Medical College, Sialkot. Period: January 2018 to June 2018. Material & Methods: 100 First year students of Islam Medical College were involved in the study. Two groups of 50 students each were made. Group 1 was exposed to the methodology of spotting. Students of group 2 were taught with One Minute Preceptor Model, but were allowed to do spotting/ structure identification themselves. Both groups were exposed to the same practical exam at the end of the course. Means of achieved marks and numbers of failures and passes were compared. All Statistical tests were applied using SPSS Version 20. Students were also asked to fill a questionnaire to find out students perception regarding the superiority of one teaching tool over the other. Results: The post hoc analysis of the result was done. The means of overall collective marks obtained by students of group I were insignificant when compared with the same of group II. Comparison of students on the basis of the passing status, carried out using Chi Square test also showed insignificant difference. On the other hand, from the questionnaires it was observed that active knowledge construction was more with OMP tool as compared to spotting. Understanding level was superior with OMP. Preparation for clinical teaching was also better with a combination of both as compared to spotting only and OMP. Conclusions: The one minute preceptor (OMP) is a time efficient teaching technique for learner centered training. It is a technique which can help the anatomy teachers to convert structure identification questions into active learning opportunities for learners. Teaching Anatomy using OMP in combination with spotting is a better tool in reshaping the subject.
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Elbagary, Ramzia I., Marwa A. Fouad, and Menna I. Ezzeldin. "Quantitative Nuclear Magnetic Resonance Spectroscopic Analysis of Two Commonly Used Gastrointestinal Tract Drugs." Journal of AOAC INTERNATIONAL 103, no. 5 (March 10, 2020): 1208–14. http://dx.doi.org/10.1093/jaoacint/qsaa036.
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Background Two fast and precise quantitative nuclear magnetic resonance spectroscopic methods (qNMR) were established and evaluated for the determination of ondansetron (OND) and omeprazole (OMP) in bulk drugs and their pharmaceutical dosage forms. NMR spectra were established using dimethylsulfoxide (DMSO-d6) as a solvent and phloroglucinol as the internal standard. Proton NMR signals at 3.743, 3.811, and 5.670 ppm were used for quantitative monitoring purposes corresponding to OND, OMP, and phloroglucinol, respectively. In this study, the methods linearity, accuracy, limit of quantification, limit of detection, stability, and precision were validated as per International Conference on Harmonization (ICH) guidelines. Linearity ranges were 0.3–10 mg for OND and 1–10 mg for OMP. The student t-test and F-test were used for statistical evaluation. Herein, the proposed methods are useful and can be a successful practical appliance for OND and OMP determination in drug substances and their dosage forms.