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1
Spies, C. F. J., L. Mostert, A. Carlucci, P. Moyo, W. J. van Jaarsveld, I. L. du Plessis, M. van Dyk, and F. Halleen. "Dieback and decline pathogens of olive trees in South Africa." Persoonia - Molecular Phylogeny and Evolution of Fungi 45, no. 1 (December 31, 2020): 196–220. http://dx.doi.org/10.3767/persoonia.2020.45.08.
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Trunk disease fungal pathogens reduce olive production globally by causing cankers, dieback, and other decline-related symptoms on olive trees. Very few fungi have been reported in association with olive dieback and decline in South Africa. Many of the fungal species reported from symptomatic olive trees in other countries have broad host ranges and are known to occur on other woody host plants in the Western Cape province, the main olive production region of South Africa. This survey investigated the diversity of fungi and symptoms associated with olive dieback and decline in South Africa. Isolations were made from internal wood symptoms of 145 European and 42 wild olive trees sampled in 10 and 9 districts, respectively. A total of 99 taxa were identified among 440 fungal isolates using combinations of morphological and molecular techniques. A new species of Pseudophaeomoniella, P. globosa, had the highest incidence, being recovered from 42.8 % of European and 54.8 % of wild olive samples. This species was recovered from 9 of the 10 districts where European olive trees were sampled and from all districts where wild olive trees were sampled. Members of the Phaeomoniellales (mainly P. globosa ) were the most prevalent fungi in five of the seven symptom types considered, the only exceptions being twig dieback, where members of the Botryosphaeriaceae were more common, and soft/white rot where only Basidiomycota were recovered. Several of the species identified are known as pathogens of olives or other woody crops either in South Africa or elsewhere in the world, including species of Neofusicoccum, Phaeoacremonium, and Pleurostoma richardsiae. However, 81 of the 99 taxa identified have not previously been recorded on olive trees and have unknown interactions with this host. These taxa include one new genus and several putative new species, of which four are formally described as Celerioriella umnquma sp. nov., Pseudophaeomoniella globosa sp. nov., Vredendaliella oleae gen. & sp. nov., and Xenocylindrosporium margaritarum sp. nov.
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Sánchez-Hernández, M. E., A. Ruiz-Dávila, and A. Trapero-Casas. "First Report of Phytophthora megasperma and Pythium irregulare As Olive Tree Root Pathogens." Plant Disease 81, no. 10 (October 1997): 1216. http://dx.doi.org/10.1094/pdis.1997.81.10.1216b.
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Several species of the genus Phytophthora are associated with root rot and trunk cankers in olive trees (Olea europaea L.). Among them, Phytophthora megasperma has been cited as being associated with olive root rots in Greece (1). Unidentified species of Pythium and Phytophthora have also been associated with olive tree root rots in the United States. However, the status of P. megasperma and Pythium spp. as olive tree root pathogens has remained unclear. Following a 5-year period of severe drought in southern Spain, autumn-winter rainfall rates in 1996 to 1997 steadily increased in both quantity and frequency. Under these unusually wet conditions, olive trees remained waterlogged for several months. During this period, we observed foliar wilting, dieback, and death of young trees, and later found extensive root necrosis. In 46 of 49 affected plantations surveyed, P. megasperma was consistently isolated from the rotted rootlets, particularly in young (<1- to 10-year-old trees) plantations. This fungus was not detected on plant material affected by damping-off from several Spanish olive tree nurseries. The opposite situation occurred with P. irregulare. This species was not associated with rotted rootlets in the field. In contrast, it was consistently isolated from necrotic rootlets from young olive plants affected by damping-off. These plants were grown in a sand-lime-peat soil mixture under greenhouse conditions and showed foliar wilting and extensive necrosis of the root systems. Pathogenicity tests were conducted with several isolates of P. megasperma and P. irregulare on 6-month-old rooted cuttings of olive, under both weekly watering and waterlogged conditions. Under waterlogged conditions, both fungal species produced extensive root necrosis 2 weeks after inoculation that resulted in wilting of the aerial parts and rapid plant death. Waterlogged control plants remained without foliar symptoms but a low degree of root necrosis was recorded. In addition, under weekly watering conditions, plants inoculated with either species showed some degree of root rot but foliar symptoms were not evident. No differences in pathogenicity were observed within the Phytophthora or Pythium isolates. Reference: (1) H. Kouyeas and A. Chitzanidis. Ann. Inst. Phytopathol. Benaki 8:175, 1968.
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Kraus, C., U. Damm, S. Bien, R. T. Voegele, and M. Fischer. "New species of Phaeomoniellales from a German vineyard and their potential threat to grapevine (Vitis vinifera) health." Fungal Systematics and Evolution 6, no. 1 (December 15, 2020): 139–55. http://dx.doi.org/10.3114/fuse.2020.06.08.
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Recently, the order Phaeomoniellales was established that includes fungi closely related to Phaeomoniella chlamydospora, a phytopathogen assumed to be the main causal agent of the two most destructive grapevine trunk diseases, Petri disease and esca. Other species of this order are reported as pathogens of other economically important crops, like olive, peach, apricot, cherry, plum, rambutan, lichee or langsat. However, they are rarely isolated and hence, little is known about their ecological traits and pathogenicity. During a 1-yr period of spore trapping in a German vineyard divided in minimally and intensively pruned grapevines, 23 fungal strains of the Phaeomoniellales were collected. Based on morphological and molecular (ITS, LSU and tub2) analyses the isolated strains were assigned to eight different species. Two species were identified as P. chlamydospora and Neophaeomoniella zymoides, respectively. The remaining six species displayed morphological and molecular differences to known species of the Phaeomoniellales and are newly described, namely Aequabiliella palatina, Minutiella simplex, Moristroma germanicum, Mo. palatinum, Neophaeomoniella constricta and N. ossiformis. A pathogenicity test conducted in the greenhouse revealed that except for P. chlamydospora, none of the species of the Phaeomoniellales isolated from spore traps is able to induce lesions in grapevine wood.
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Lo Giudice, V., F. Raudino, R. Magnano di San Lio, S. O. Cacciola, R. Faedda, and A. Pane. "First Report of a Decline and Wilt of Young Olive Trees Caused by Simultaneous Infections of Verticillium dahliae and Phytophthora palmivora in Sicily." Plant Disease 94, no. 11 (November 2010): 1372. http://dx.doi.org/10.1094/pdis-07-10-0480.
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In summer 2008, leaf chlorosis, defoliation, exceptional fruit set, twig dieback, and wilt were observed on 4-year-old olive (Olea europea L.) trees cv. Tonda Iblea in a drip-irrigated orchard in eastern Sicily. Rot of fine roots was associated with these symptoms and on ~15% of symptomatic trees rot extended to the crown and basal stem. Trees declined slowly or collapsed suddenly with withered leaves still attached. Incidence of affected trees was ~10%. A fungus identified as Verticillium dahliae Kleb. was isolated from the xylem of main roots and basal stem. An oomycete identified as Phytophthora palmivora (Butler) Butler was isolated from roots and basal trunk bark. Both pathogens were recovered from symptomatic trees with mean frequency of positive isolations per tree of 80 and 30% for V. dahliae and P. palmivora, respectively. To isolate V. dahliae, wood chips were surface disinfested in 0.5% NaOCl for 1 min and plated onto potato dextrose agar (PDA). The fungus was identified on the basis of microsclerotia, verticillate arrangement of phialides on conidiophores, and hyaline single-celled conidia. Ten monoconidial isolates were characterized by PCR using primer pairs INTND2f/INTND2r and DB19/espdef01 (3). Only 824-bp amplicons, diagnostic of the virulent, nondefoliating V. dahliae pathotype, were obtained. P. palmivora was isolated on selective medium (2) and pure cultures were obtained by single-hypha transfers. Colonies grew on PDA between 10 and 35°C (optimum at 27°C). Chlamydospores and elliptical to ovoid, papillate, caducous (mean pedicel length = 5 μm) sporangia (length/breadth ratio of 1.8) were produced on V8 juice agar. All isolates were paired with reference isolates of P. nicotianae and produced gametangia only with isolates of the A2 mating type. PCR amplicons of a representative isolate generated using primers ITS 6 and ITS 4 (1) were sequenced and found to be identical to those of a reference isolate of P. palmivora (GenBank No. AY208126). Pathogenicity of V. dahliae (IMI 397476) and P. palmivora (IMI 397475) was tested on 6-month-old rooted cuttings of olive cv. Tonda Iblea. Ten cuttings were transplanted into pots with steam-sterilized soil and inoculum of P. palmivora (4% vol/vol) produced on wheat kernels. Ten olive cuttings were inoculated with V. dahliae by injecting the stem with 150 μl of a conidial suspension (107 conidia ml–1) and 10 cuttings were stem inoculated with V. dahliae and transplanted into soil infested with P. palmivora. Controls were 10 noninoculated cuttings transplanted into steam-sterilized soil. Pots were kept in a greenhouse (25 ± 3°C) for 4 months. No aerial symptoms were observed on cuttings transplanted into soil infested with P. palmivora. However, root dry weight was reduced by 40% in comparison with the controls. Cuttings inoculated solely with V. dahliae had a 15% reduction in height compared with the controls but only four cuttings wilted. All cuttings inoculated with P. palmivora and V. dahliae wilted, indicating a synergism between the two pathogens. Controls remained healthy. Each pathogen was reisolated solely from inoculated cuttings and both pathogens were reisolated from cuttings with double inoculations. A similar syndrome ‘seca’ (drying) was reported in Spain (4). References: (1) D. E. L. Cooke et al. Fungal Genet. Biol. 30:17, 2000. (2) H. Masago et al. Phytopathology 67:425, 1977. (3) J. Mercado-Blanco et al. Plant Dis. 87:1487, 2003. (4) M. E. Sánchez-Hernández et al. Eur. J. Plant Pathol. 104:34, 1998.
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Granata, G., R. Faedda, and A. Sidoti. "First Report of Canker Disease Caused by Diplodia olivarum on Carob Tree in Italy." Plant Disease 95, no. 6 (June 2011): 776. http://dx.doi.org/10.1094/pdis-12-10-0870.
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The evergreen carob tree (Ceratonia siliqua L., Fabaceae), also called locust, is widespread in the Mediterranean Region. Carob pods have been traditionally consumed as animal and human food and seeds are mainly used in the pharmaceutical and cosmetic industries. In July 2009, symptoms of canker, branch dieback, and foliage reddening were observed on carob trees in several natural areas in the province of Ragusa, Italy. Disease incidence ranged from 5 to 80% across different sites and for most areas it was nearly 15%. All affected trees showed dark necrotic tissue in the bark, cambium, and sapwood of the trunk and branches. Cankers often girdled the stem or branch, causing wilting and death of the portions beyond the canker. Black, subepidermal pycnidia developed in and erupted through the dead bark. Fragments of discolored wood were collected from 36 symptomatic carob trees (12 trees for each area), transferred onto potato dextrose agar (PDA), and incubated for 5 days at 21°C in the dark. Fungal colonies were consistently obtained from these diseased tissues. They initially were pale, becoming gray-green and finally black. After 30 days of incubation at room temperature in the natural light, colonies produced pycnidia identical to those observed in nature. A total of 500 conidia on 10 isolates were examined with a compound microscope. Conidia were initially hyaline, smooth, oblong to ovoid, both ends rounded, and aseptate; at maturity they were pale brown, one-septate, and measured 24 to 28 × 10 to 13.5 μm (means ± S.D. = 24.3 ± 1.4 × 12.1 ± 1 μm, L/W = 2.0 ± 0.18). The nucleotide sequences of the β-tubulin (GenBank Accession No. HQ660080) and TE-1α (No. HQ660078) genes and ITS-rDNA region (No. HM028640) for a representative isolate (IMI 390972) from carob showed 100, 100, and 98% similarity, respectively, when compared with the sequences HQ660079, EU392279, and EU392302, respectively, of the ex-type isolate of Diplodia olivarum (strain CBS 121887). On the basis of morphological and molecular characters, the fungus was identified as D. olivarum A.J.L. Phillips, Frisullo & Lazzizera; teleomorph unknown (1). Two-year-old trees were wounded with a scalpel through the full thickness of the bark along 1-cm longitudinal direction and inoculated by applying a 5-mm-diameter plug of mycelial (isolate IMI 390972) on PDA to the wound site. Three control seedlings were similarly wounded and plugs of sterile PDA applied. Plugs were held in place by Parafilm. The inoculated seedlings were maintained at 20 to 22°C and a 12-h light/dark cycle. Sixty days after inoculation, all inoculated trees showed leaf chlorosis, sunken, necrotic bark at the inoculation sites and finally pycnidia of D. olivarum. All treated seedlings were killed within 6 months from the inoculation. No symptoms were observed in the control plants. The pathogen was consistently reisolated from all the inoculated trees, but not from the control plants. D. olivarum has been found on rotting olive drupes in Apulia (southern Italy) and was first described as a new species in 2008 (1). This fungal species could be phenotypically misidentified as the closely related species D. mutila, which differs by having larger mean dimensions of conidia. To our knowledge, this is the first report of D. olivarum inducing canker and dieback on carob tree. Reference: (1) C. Lazzizera et al. Fungal Divers. 31:63, 2008.
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Peduto Hand, F., R. A. Choudhury, and W. D. Gubler. "First Report of Cytospora punicae Causing Wood Canker and Branch Dieback of Pomegranate (Punica granatum) in the United States." Plant Disease 98, no. 6 (June 2014): 853. http://dx.doi.org/10.1094/pdis-11-13-1133-pdn.
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Pomegranates (Punica granatum L.) are an expanding industry in the United States with California growing approximately 32,000 acres with a crop value of over $155 million (1). During June and July of 2012, we observed severe limb and branch dieback in pomegranate orchards cv. Wonderful located in Contra Costa, Kings, and Kern counties of California. Disease symptoms included yellowing of leaves, branch and limb dieback, wood lesions, and canker formation. Dark brown Cytospora-like cultures were consistently isolated from active cankers on potato dextrose agar (PDA) amended with 100 mg l−1 tetracycline hydrochloride. Three isolates (UCCE1223, UCCE1233, and UCCE1234) representative of each orchard were sub-cultured onto PDA and incubated at 22°C under fluorescent intermittent light (12 h light, 12 h dark). Fungal colonies had whitish mycelia that turned olive green to dark brown with maturity and formed globose and dark brown pycnidia after 12 days. Conidia were hyaline, aseptate, allantoid, and (4) 4.5 to 5 (6) × (1) 1.5 (2) μm (n = 180). Pycnidia formed in culture measured (250) 350 to 475 (650) μm in diameter (n = 40). Identification of the isolates was confirmed by sequence comparison of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rDNA and part of the translation elongation factor 1-α gene (EF1-α) with sequences available in GenBank. Consensus sequences of both genes of all isolates showed 99% homology to the species Cytospora punicae Sacc. (2). All sequences were deposited in GenBank (Accession Nos. KJ621684 to 89). Pathogenicity of the isolates was determined by branch inoculation. In December 2012, 3-year-old branches of P. granatum cv. Wonderful were inoculated by placing 5-mm-diameter mycelium plugs from the growing margin of 14-day-old PDA cultures in fresh wounds made with a 5-mm-diameter cork-borer. Eight branches per isolate were inoculated on eight different trees. Eight control branches were inoculated with non-colonized PDA agar plugs. Inoculations were covered with Vaseline and wrapped with Parafilm to retain moisture. Branches were harvested in August 2013 and examined for canker development and the extent of vascular discoloration spreading downward and upward from the inoculation point. Isolations from the edge of discolored tissue were conducted to fulfill Koch's postulates. C. punicae was re-isolated from 100% of the inoculated branches. Total length of vascular discoloration averaged 30.2 mm in branches inoculated with the three C. punicae isolates and 9 mm in the control branches. No fungi were isolated from the slightly discolored tissue of the controls. To our knowledge, this is the first report of C. punicae as a fungal trunk pathogen of pomegranate trees in the United States. References: (1) California County Agricultural Commissioners' Data, 2010 Crop Year. USDA NASS California field office, retrieved from http://www.nass.usda.gov/Statistics_by_State/California/ Publications/AgComm/201010cactb00.pdf , 2011. (2) P. A. Saccardo. Sylloge Fungorum 3:256, 1884.
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Varanda, Carla M. R., Patrick Materatski, Miguel Landum, Maria Doroteia Campos, and Maria do Rosário Félix. "Fungal Communities Associated with Peacock and Cercospora Leaf Spots in Olive." Plants 8, no. 6 (June 12, 2019): 169. http://dx.doi.org/10.3390/plants8060169.
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Venturia oleaginea and Pseudocercospora cladosporioides are two of the most important olive fungal pathogens causing leaf spots: peacock spot, and cercosporiosis, respectively. In the present study, fungal communities associated with the presence of these pathogens were investigated. Overall, 300 symptomatic and asymptomatic trees from different cultivars were sampled from Alentejo, Portugal. A total of 788 fungal isolates were obtained and classified into 21 OTUs; Ascomycota was clearly the predominant phylum (96.6%). Trees from cultivar ‘Galega vulgar’ showed a significant higher fungal richness when compared to ‘Cobrançosa’, which in turn showed significant higher values than ‘Picual’. Concerning plant health status, symptomatic plants showed significant higher fungal richness, mainly due to the high number of isolates of the pathogens V. oleaginea and P. cladosporioides. In terms of fungal diversity, there were two major groups: ca. 90% of the isolates found in symptomatic plants belonged to V. oleaginea, P. cladosporioides, Chalara sp., and Foliophoma sp. while ca. 90% of the isolates found in asymptomatic plants, belonged to Alternaria sp. and Epicoccum sp. This study highlights the existence of different fungal communities in olive trees, including potential antagonistic organisms that can have a significant impact on diseases and consequently on olive production.
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Eichmeier, A., J. Pecenka, M. Spetik, T. Necas, I. Ondrasek, J. Armengol, M. León, C. Berlanas, and D. Gramaje. "Fungal Trunk Pathogens Associated With Juglans regia in the Czech Republic." Plant Disease 104, no. 3 (March 2020): 761–71. http://dx.doi.org/10.1094/pdis-06-19-1308-re.
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Juglans regia L. (English walnut) trees with cankers and dieback symptoms were observed in two regions in the Czech Republic. Isolations were made from diseased branches. In total, 138 fungal isolates representing 10 fungal species were obtained from wood samples and identified based on morphological characteristics and molecular methods: Cadophora novi-eboraci, Cadophora spadicis, Cryptovalsa ampelina, Diaporthe eres, Diplodia seriata, Dothiorella omnivora, Eutypa lata, Eutypella sp., Peroneutypa scoparia, and Phaeoacremonium sicilianum. Pathogenicity tests conducted under field conditions with all species using the mycelium-plug method indicated that Eutypa lata and Cadophora spp. were highly virulent to woody stems of walnut. This is the first study to detect and identify fungal trunk pathogens associated with diseased walnut trees in Europe.
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Panahandeh, Saleh, Hamid Mohammadi, and David Gramaje. "Trunk Disease Fungi Associated with Syzygium cumini in Iran." Plant Disease 103, no. 4 (April 2019): 711–20. http://dx.doi.org/10.1094/pdis-06-18-0923-re.
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Syzygium cumini trees with dieback symptoms and cankers were observed in two provinces in Iran. Isolations were made from diseased branches and cankers and from asymptomatic S. cumini wood samples. Several trunk disease pathogens were identified based on morphological characteristics and by molecular methods, including Cadophora luteo-olivacea, Diplodia sapinea, D. seriata, Neoscytalidium hyalinum, Phaeoacremonium fraxinopennsylvanicum, P. krajdenii, P. parasiticum, P. viticola, and Pleurostoma richardsiae, which were isolated from S. cumini for the first time in the world. Pathogenicity tests conducted with all species confirmed their status as possible S. cumini pathogens. N. hyalinum was the most aggressive species and caused the longest lesions on inoculated shoots. The endophytic character of some fungal species isolated from asymptomatic wood of S. cumini is further discussed. Our results indicated that S. cumini is a new woody host to many known fungal trunk pathogens.
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Mohammadi, Hamid, Mehdi Sarcheshmehpour, and Ebrahim Mafi. "Fungal trunk pathogens associated with wood decay of pistachio trees in Iran." Spanish Journal of Agricultural Research 13, no. 2 (May 29, 2015): e1007. http://dx.doi.org/10.5424/sjar/2015132-6560.
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Over the growing seasons of 2011–2013, various pistachio (<em>Pistacia vera</em> L.) cv. Fandoghi, and wild pistachio (<em>P. atlantica </em>Desf. subsp. <em>mutica</em>) trees were inspected in Iran to determine the aetiology of trunk diseases with specific reference to species of <em>Phaeoacremonium</em> and Botryosphaeriaceae spp. Samples were collected from branches of trees exhibiting yellowing, defoliation, canker and dieback, as well as wood discoloration in cross sections. Fungal trunk pathogens were identified using morphological and cultural characteristics as well as comparisons of DNA sequence data of the ITS and TEF-1α (for Botryosphaeriaceae species) and β-tubulin gene (for <em>Phaeoacremonium</em> species) regions. <em>Phaeoacremonium parasiticum</em> was the dominant species followed by <em>Phaeoacremonium aleophilum</em>, <em>Botryosphaeria dothidea</em>,<em> Neofusicoccum parvum</em>,<em> </em><em>Phaeoacremonium</em> <em>cinereum, Phaeoacremonium viticola</em> and <em>Dothiorella viticola</em>. Pathogenicity tests were undertaken to determine the role of these species on pistachio under field conditions. <em>Neofusicoccum parvum</em> and <em>Pm. aleophilum</em> caused the longest and smallest lesions respectively. This study represents the first report on the occurrence and pathogenicity of <em>Phaeoacremonium </em>species on <em>P. vera</em> cv. Fandoghi. This also represents the first report of <em>Pleurostomophora </em>sp. on pistachio and <em>Pm. parasiticum</em> and <em>D</em>.<em> viticola</em> on wild pistachio.
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Cheffi, Manel, Ali Chenari Bouket, Faizah N. Alenezi, Lenka Luptakova, Marta Belka, Armelle Vallat, Mostafa E. Rateb, Slim Tounsi, Mohamed Ali Triki, and Lassaad Belbahri. "Olea europaea L. Root Endophyte Bacillus velezensis OEE1 Counteracts Oomycete and Fungal Harmful Pathogens and Harbours a Large Repertoire of Secreted and Volatile Metabolites and Beneficial Functional Genes." Microorganisms 7, no. 9 (September 3, 2019): 314. http://dx.doi.org/10.3390/microorganisms7090314.
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Oomycete and fungal pathogens, mainly Phytophthora and Fusarium species, are notorious causal agents of huge economic losses and environmental damages. For instance, Phytophthora ramorum, Phytophthora cryptogea, Phytophthora plurivora and Fusarium solani cause significant losses in nurseries and in forest ecosystems. Chemical treatments, while harmful to the environment and human health, have been proved to have little or no impact on these species. Recently, biocontrol bacterial species were used to cope with these pathogens and have shown promising prospects towards sustainable and eco-friendly agricultural practices. Olive trees prone to Phytophthora and Fusarium disease outbreaks are suitable for habitat-adapted symbiotic strategies, to recover oomycetes and fungal pathogen biocontrol agents. Using this strategy, we showed that olive trees-associated microbiome represents a valuable source for microorganisms, promoting plant growth and healthy benefits in addition to being biocontrol agents against oomycete and fungal diseases. Isolation, characterization and screening of root microbiome of olive trees against numerous Phytophthora and other fungal pathogens have led to the identification of the Bacillus velezensis OEE1, with plant growth promotion (PGP) abilities and strong activity against major oomycete and fungal pathogens. Phylogenomic analysis of the strain OEE1 showed that B. velezensis suffers taxonomic imprecision that blurs species delimitation, impacting their biofertilizers’ practical use. Genome mining of several B. velezensis strains available in the GenBank have highlighted a wide array of plant growth promoting rhizobacteria (PGPR) features, metals and antibiotics resistance and the degradation ability of phytotoxic aromatic compounds. Strain OEE1 harbours a large repertoire of secreted and volatile secondary metabolites. Rarefaction analysis of secondary metabolites richness in the B. velezenis genomes, unambiguously documented new secondary metabolites from ongoing genome sequencing efforts that warrants more efforts in order to assess the huge diversity in the species. Comparative genomics indicated that B. velezensis harbours a core genome endowed with PGP features and accessory genome encoding diverse secondary metabolites. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of OEE1 Volatile Organic Compounds (VOCs) and Liquid Chromatography High Resolution Mas Spectrometry (LC-HRMS) analysis of secondary metabolites identified numerous molecules with PGP abilities that are known to interfere with pathogen development. Moreover, B. velezensis OEE1 proved effective in protecting olive trees against F. solani in greenhouse experiments and are able to inhabit olive tree roots. Our strategy provides an effective means for isolation of biocontrol agents against recalcitrant pathogens. Their genomic analysis provides necessary clues towards their efficient implementation as biofertilizers.
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Gramaje, D., C. Agustí-Brisach, A. Pérez-Sierra, E. Moralejo, D. Olmo, L. Mostert, U. Damm, and J. Armengol. "Fungal trunk pathogens associated with wood decay of almond trees on Mallorca (Spain)." Persoonia - Molecular Phylogeny and Evolution of Fungi 28, no. 1 (June 30, 2012): 1–13. http://dx.doi.org/10.3767/003158512x626155.
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Ammad, Faiza, Messaoud Benchabane, and Mohamed Toumi. "Diversity of Fungal Trunk Pathogens Associated with Grapevine Dieback of Grapevine in Algeria." Jordan Journal of Biological Sciences 7, no. 1 (March 2014): 35–39. http://dx.doi.org/10.12816/0008211.
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Urbez-Torres, Jose Ramon, Francesca Peduto, R. K. Striegler, K. E. Urrea-Romero, J. C. Rupe, R. D. Cartwright, and W. D. Gubler. "Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri." Fungal Diversity 52, no. 1 (June 11, 2011): 169–89. http://dx.doi.org/10.1007/s13225-011-0110-4.
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Sohrabi, Mahboobeh, Hamid Mohammadi, Maela León, Josep Armengol, and Zia Banihashemi. "Fungal pathogens associated with branch and trunk cankers of nut crops in Iran." European Journal of Plant Pathology 157, no. 2 (May 12, 2020): 327–51. http://dx.doi.org/10.1007/s10658-020-01996-w.
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Lawrence, Daniel P., Renaud Travadon, and Kendra Baumgartner. "Novel Seimatosporium Species from Grapevine in Northern California and Their Interactions with Fungal Pathogens Involved in the Trunk-Disease Complex." Plant Disease 102, no. 6 (June 2018): 1081–92. http://dx.doi.org/10.1094/pdis-08-17-1247-re.
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Seimatosporium spp. and closely related “pestalotioid fungi” have been isolated from vineyards worldwide, but their ecological status in grapevine wood is unclear. To determine their involvement in the grapevine trunk-disease complex, we tested the pathogenicity of Californian isolates obtained from vines with general symptoms of Botryosphaeria, Eutypa, and Phomopsis diebacks. Multilocus phylogenetic analyses revealed three species: Seimatosporium vitis and two newly described and typified species, S. luteosporum sp. nov. and S. vitifusiforme sp. nov. Inoculations to woody stems of potted grapevines of both isolates of S. vitis and one isolate of S. vitifusiforme, but not S. luteosporum, were associated with significantly larger lesions than those of noninoculated controls. Coinoculations with trunk pathogens (Cryptovalsa ampelina, Diaporthe ambigua, Diatrypella verruciformis, Diplodia seriata, and Eutypa lata), coisolated from the same wood cankers in the field, brought about increased lesion lengths for S. vitifusiforme paired with D. seriata, and S. luteosporum paired with Diaporthe ambigua. In contrast, there were no differences in lesion lengths of S. vitis and Diatrypella verruciformis or S. vitis and E. lata, inoculated alone or together. Our findings suggest that Seimatosporium spp. are involved in the grapevine trunk-disease complex, and their virulence may depend on or affect that of trunk pathogens.
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Cobos, Rebeca, Rosa María Mateos, José Manuel Álvarez-Pérez, Miguel Angel Olego, Silvia Sevillano, Sandra González-García, Enrique Garzón-Jimeno, and Juan José R. Coque. "Effectiveness of Natural Antifungal Compounds in Controlling Infection by Grapevine Trunk Disease Pathogens through Pruning Wounds." Applied and Environmental Microbiology 81, no. 18 (July 10, 2015): 6474–83. http://dx.doi.org/10.1128/aem.01818-15.
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ABSTRACTGrapevine trunk fungal pathogens, such asDiplodia seriataandPhaeomoniella chlamydospora, can infect plants through pruning wounds. They cause grapevine trunk diseases and are involved in grapevine decline. Accordingly, the protection of pruning wounds is crucial for the management of grapevine trunk diseases. The efficacy of different natural antifungals in inhibiting the growth of several fungi causing grapevine trunk diseases was evaluatedin vitro. The fungi showing greaterin vitroefficacy were tested on autoclaved grape wood assays againstD. seriataandP. chlamydospora. Based on results from these assays, chitosan oligosaccharide, vanillin, and garlic extract were selected for further evaluation on pruning wounds inoculated withD. seriataandP. chlamydosporain field trials. A significant decrease in plant mortality was observed after 2 years of growth in the plants treated with the different natural antifungals compared to the mortality rate observed in infected plants that were not treated with antifungals. Also, the infection rate for the inoculated pathogens was significantly reduced in plants treated with the selected natural antifungals. Therefore, natural antifungals represent a promising alternative for disease control and could provide significant economic benefits for the grape-growing industry.
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Magnin-Robert, Maryline, Alessandro Spagnolo, Anna Boulanger, Cécile Joyeux, Christophe Clément, Eliane Abou-Mansour, and Florence Fontaine. "Changes in Plant Metabolism and Accumulation of Fungal Metabolites in Response to Esca Proper and Apoplexy Expression in the Whole Grapevine." Phytopathology® 106, no. 6 (June 2016): 541–53. http://dx.doi.org/10.1094/phyto-09-15-0207-r.
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Trunk diseases have become among the most important grapevine diseases worldwide. They are caused by fungal pathogens that attack the permanent woody structure of the vines and cause various symptoms in woody and annual organs. This study examined modifications of plant responses in green stem, cordon, and trunk of grapevines expressing Esca proper (E) or apoplexy (A) event, which are the most frequent grapevine trunk disease symptoms observed in Europe. Transcript expression of a set of plant defense- and stress-related genes was monitored by quantitative reverse-transcription polymerase chain reaction while plant phytoalexins and fungal metabolites were quantified by high-performance liquid chromatography-mass spectrometry in order to characterize the interaction between the grapevine and trunk disease agents. Expression of genes encoding enzymes of the phenylpropanoid pathway and trans-resveratrol content were altered in the three organs of diseased plants, especially in the young tissues of A plants. Pathogenesis-related proteins and the antioxidant system were severely modulated in A plants, which indicates a drastic stress effect. In the meantime, fungal polyketides 6-MSA, (R)-mellein, and (3R,4R)-4-hydroxymellein, were accumulated in A plants, which suggests their potential effect on plant metabolism during the appearance of foliar symptoms.
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Fernández-González, Mónica, and Pedro Miguel Izquierdo Cañas. "Identification of fungal pathogens associated with grapevine trunk using fluorescent-labelled ribosomal DNA probe." Archives of Phytopathology and Plant Protection 49, no. 19-20 (October 17, 2016): 567–74. http://dx.doi.org/10.1080/03235408.2016.1244000.
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Travadon, Renaud, Philippe E. Rolshausen, Walter D. Gubler, Lance Cadle-Davidson, and Kendra Baumgartner. "Susceptibility of Cultivated and Wild Vitis spp. to Wood Infection by Fungal Trunk Pathogens." Plant Disease 97, no. 12 (December 2013): 1529–36. http://dx.doi.org/10.1094/pdis-05-13-0525-re.
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Cultivars of European grapevine, Vitis vinifera, show varying levels of susceptibility to Eutypa dieback and Esca, in terms of foliar symptoms. However, little is known regarding cultivar susceptibility of their woody tissues to canker formation. Accordingly, we evaluated the relative susceptibility of V. vinifera cultivars (‘Cabernet Franc’, ‘Cabernet Sauvignon’, ‘Chardonnay’, ‘Merlot’, ‘Riesling’, ‘Petite Syrah’, and ‘Thompson Seedless’) and species or interspecific hybrids of North American Vitis (Vitis hybrid ‘Concord’, V. arizonica ‘b42-26’, V. rupestris × V. cinerea ‘Ill547-1’, and Fennell 6 [V. aestivalis] × Malaga [V. vinifera] ‘DVIT0166’) to canker formation by seven trunk pathogens (Neofusicoccum parvum, Lasiodiplodia theobromae, Phaeomoniella chlamydospora, Togninia minima, Phomopsis viticola, Eutypa lata, and an undescribed Eutypa sp.). Susceptibility was based on the length of wood discoloration (LWD) in the woody stems of rooted plants in duplicate greenhouse experiments. Cultivars of V. vinifera and Concord did not vary significantly in susceptibility to N. parvum or L. theobromae (LWD of 21 to 88 mm at 14 weeks post inoculation; P > 0.16), suggesting that they are similarly susceptible to Botryosphaeria dieback. The table-grape Thompson Seedless was most susceptible to P. viticola (mean LWD of 61 mm at 11 months post inoculation; P < 0.0001). V. vinifera cultivars and Concord showed similar susceptibility to the Esca pathogens, Phaeomoniella chlamydospora and T. minima. Susceptibility to E. lata was greatest in V. arizonica b42-26 (mean LWD of 96 mm at 11 months post inoculation; P < 0.03). In fact, all four American Vitis spp. were more susceptible to Eutypa dieback than the V. vinifera cultivars. Our findings suggest that no one cultivar is likely to provide resistance to the range of trunk pathogens but that certain cultivars may be promising candidates for commercially relevant host resistance in grape-production systems where the dominant cultivars are very susceptible.
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Agustí-Brisach, C., M. León, J. García-Jiménez, and J. Armengol. "Detection of Grapevine Fungal Trunk Pathogens on Pruning Shears and Evaluation of Their Potential for Spread of Infection." Plant Disease 99, no. 7 (July 2015): 976–81. http://dx.doi.org/10.1094/pdis-12-14-1283-re.
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Four vineyards visibly affected by trunk diseases were surveyed at pruning time in 2012 and 2013 in Spain, to determine whether pruning tools are capable of spreading grapevine trunk diseases from vine to vine. In each vineyard, pruning shears were regularly rinsed with sterile water, collecting liquid samples for analysis. Molecular detection of grapevine fungal trunk pathogens (GFTPs) was performed by nested polymerase chain reaction using specific primers to detect Botryosphaeriaceae spp. Eutypa lata, Cadophora luteo-olivacea, Phaeoacremonium spp., and Phaeomoniella chlamydospora. All of these GFTPs, with the exception of E. lata, were detected in samples from the four vineyards, C. luteo-olivacea and Phaeoacremonium spp. being the most prevalent. Co-occurrence of two, three, or four different GFTPs from the same sample were found, the simultaneous detection of C. luteo-olivacea and Phaeoacremonium spp. being the most prevalent. In addition, fungal isolation from liquid samples in semiselective culture medium for C. luteo-olivacea, Phaeoacremonium spp., and P. chlamydospora was also performed but only C. luteo-olivacea was recovered from samples collected in three of four vineyards evaluated. Pruning shears artificially infested with suspensions of conidia or mycelial fragments of C. luteo-olivacea, Diplodia seriata, E. lata, Phaeoacremonium aleophilum, and Phaeomoniella chlamydospora were used to prune 1-year-old grapevine cuttings of ‘110 Richter’ rootstock. Successful fungal reisolation from the cuttings 4 months after pruning confirmed that infested pruning shears were able to infect them through pruning wounds. These results improve knowledge about the epidemiology of GFTPs and demonstrate the potential of inoculum present on pruning shears to infect grapevines.
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Elena, Georgina, and Jordi Luque. "Seasonal Susceptibility of Grapevine Pruning Wounds and Cane Colonization in Catalonia, Spain Following Artificial Infection with Diplodia seriata and Phaeomoniella chlamydospora." Plant Disease 100, no. 8 (August 2016): 1651–59. http://dx.doi.org/10.1094/pdis-10-15-1186-re.
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Diplodia seriata and Phaeomoniella chlamydospora are two fungal pathogens associated with grapevine trunk diseases worldwide. This study aimed to evaluate the period during which grapevine pruning wounds remain susceptible to fungal infection and to describe the colonization of canes artificially inoculated with these pathogens. In the first experiment, pruning wounds made in either fall or winter were separately inoculated with each pathogen at different times after pruning. Wound susceptibility to both pathogens decreased as the period between pruning and inoculation increased, from high percentages recorded in the first inoculation round (D. seriata, 97.5% and P. chlamydospora, 75%) down to approximately 10% 12 weeks after pruning. Pruning wounds remained more susceptible to D. seriata after a late pruning in winter whereas no overall seasonal changes in wound susceptibility were detected for P. chlamydospora. In the second experiment, canes were pruned by leaving two different lengths between the top node and the pruning wound before inoculations. Pathogens were recovered at different incubation periods and from different sites along the canes to estimate fungal cane colonization. A longer pruned internode made cane colonization by P. chlamydospora difficult, as indicated by fungal recoveries lower than 10% at the lowest recovery site, whereas D. seriata was less inhibited.
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Ntougias, Spyridon, Kostas Bourtzis, and George Tsiamis. "The Microbiology of Olive Mill Wastes." BioMed Research International 2013 (2013): 1–16. http://dx.doi.org/10.1155/2013/784591.
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Olive mill wastes (OMWs) are high-strength organic effluents, which upon disposal can degrade soil and water quality, negatively affecting aquatic and terrestrial ecosystems. The main purpose of this review paper is to provide an up-to-date knowledge concerning the microbial communities identified over the past 20 years in olive mill wastes using both culture-dependent and independent approaches. A database survey of 16S rRNA gene sequences (585 records in total) obtained from olive mill waste environments revealed the dominance of members ofAlphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes,andActinobacteria. Independent studies confirmed that OMW microbial communities’ structure is cultivar dependant. On the other hand, the detection of fecal bacteria and other potential human pathogens in OMWs is of major concern and deserves further examination. Despite the fact that the degradation and detoxification of the olive mill wastes have been mostly investigated through the application of known bacterial and fungal species originated from other environmental sources, the biotechnological potential of indigenous microbiota should be further exploited in respect to olive mill waste bioremediation and inactivation of plant and human pathogens. The implementation of omic and metagenomic approaches will further elucidate disposal issues of olive mill wastes.
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YE, Qingtong, Wei ZHANG, Jingyi JIA, Xinghong LI, Yueyan ZHOU, Changping HAN, Xuehong WU, and Jiye YAN. "Fungal pathogens associated with black foot of grapevine in China." Phytopathologia Mediterranea 60, no. 2 (September 13, 2021): 303–19. http://dx.doi.org/10.36253/phyto-12353.
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Grapevine trunk diseases (GTDs) are the most destructive diseases in grape-growing regions worldwide. Black foot is one of the important GTDs affecting young vineyards and nurseries. This disease has not been reported in China. During 2017 and 2019, field surveys were carried out in the Guangxi, Hebei, Ningxia, Shanxi, and Xinjiang provinces of China. Incidence of plants with black foot symptoms was 0.1% to 1% in the surveyed vineyards. Plant samples with poorly developed shoots and canes, chlorotic leaves, and necrotic trunks or roots were collected from the five provinces. In total, 50 fungal isolates were obtained from symptomatic tissues. Based on morphological and multi-gene phylogenetic analyses, five species were identified as Cylindrocladiella lageniformis, Dactylonectria torresensis, D. macrodidyma, D. alcacerensis and Neonectria sp.1. Pathogenicity was assessed using young, healthy detached green shoots of grapevine ‘Summer Black’ and potted 3-month-old ‘Summer Black’ cuttings. Inoculated detached shoots developed necroses after 7 d, and inoculated cuttings after 80 d. Fungi were re-isolated from necrotic lesions. Among the five species, D. macrodidyma was the most aggressive. This is the first report of C. lageniformis, D. torresensis, D. macrodidyma, D. alcacerensis, and Neonectria sp. 1 associated with black foot in China. This study has enhanced knowledge of the fungi associated with black foot in China, and will assist development of control measures for this disease.
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Espargham, Nahid, Hamid Mohammadi, and David Gramaje. "A Survey of Trunk Disease Pathogens within Citrus Trees in Iran." Plants 9, no. 6 (June 16, 2020): 754. http://dx.doi.org/10.3390/plants9060754.
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Citrus trees with cankers and dieback symptoms were observed in Bushehr (Bushehr province, Iran). Isolations were made from diseased cankers and branches. Recovered fungal isolates were identified using cultural and morphological characteristics, as well as comparisons of DNA sequence data of the nuclear ribosomal DNA-internal transcribed spacer region, translation elongation factor 1α, β-tubulin, and actin gene regions. Dothiorella viticola, Lasiodiplodia theobromae, Neoscytalidium hyalinum, Phaeoacremonium (P.) parasiticum, P. italicum, P. iranianum, P. rubrigenum, P. minimum, P. croatiense, P. fraxinopensylvanicum, Phaeoacremonium sp., Cadophora luteo-olivacea, Biscogniauxia (B.) mediterranea, Colletotrichum gloeosporioides, C. boninense, Peyronellaea (Pa.) pinodella, Stilbocrea (S.) walteri, and several isolates of Phoma, Pestalotiopsis, and Fusarium species were obtained from diseased trees. The pathogenicity tests were conducted by artificial inoculation of excised shoots of healthy acid lime trees (Citrus aurantifolia) under controlled conditions. Lasiodiplodia theobromae was the most virulent and caused the longest lesions within 40 days of inoculation. According to literature reviews, this is the first report of L. theobromae and N. hyalinum on citrus in Iran. Additionally, we report several Phaeoacremonium species, S. walteri, Pa. pinodella and C. luteo-olivacea on citrus trees for the first time in the world.
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Lynch, Shannon Colleen, Paul J. Zambino, Joey Sal Mayorquin, Danny Ho Wang, and Akif Eskalen. "Identification of New Fungal Pathogens of Coast Live Oak in California." Plant Disease 97, no. 8 (August 2013): 1025–36. http://dx.doi.org/10.1094/pdis-11-12-1055-re.
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A decline of coast live oak (Quercus agrifolia) has been observed throughout southern California. In this study, the identity and pathogenicity of non-Botryosphaeriaceae fungal species consistently recovered from necrotic tissues of branch and bleeding trunk canker samples from these locations were assessed. Species were identified morphologically and by comparison of the complete sequence of the internal transcribed spacer (ITS) of the ribosomal DNA to sequences available in GenBank. Phylogenetic analyses were then conducted using ITS and partial sequences of the β-tubulin and mitochondrial small ribosomal subunit genes for unknown species. Fungi recovered and identified included Fusarium solani, Phaeoacremonium mortoniae, Diatrypella verrucaeformis, and a fungus described herein as Cryptosporiopsis querciphila sp. nov. One-year-old coast live oak seedlings were wound inoculated under controlled conditions to test pathogenicity of the fungal species. Each fungal species was successfully reisolated from necrotic tissue at 70 and 100% for P. mortoniae and all other species, and xylem necrosis was significantly different between all treatments and controls (P < 0.0001 at α = 0.05). Isolates of F. solani were the most aggressive tested. These species represent new records of fungal pathogens of coast live oak in California. Results from the pathogenicity test suggest that these fungi play a role in the decline of southern California coast live oak trees.
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Olmo, Diego, Josep Armengol, Maela León, and David Gramaje. "Pathogenicity testing of lesser-known fungal trunk pathogens associated with wood decay of almond trees." European Journal of Plant Pathology 143, no. 3 (July 2, 2015): 607–11. http://dx.doi.org/10.1007/s10658-015-0699-3.
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Moyo, P., L. Mostert, M. Bester, and F. Halleen. "Trunk Disease Fungi Associated With Diospyros kaki in South Africa." Plant Disease 100, no. 12 (December 2016): 2383–93. http://dx.doi.org/10.1094/pdis-02-16-0245-re.
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Persimmon trees with dieback symptoms and cankers were observed in three production areas in Western Cape Province in South Africa. Isolations were made from diseased branches, cankers, and pruning wounds as well as fungal fruiting bodies on dead branches and old pruning wounds. Several trunk disease pathogens were identified based on morphological characteristics and by molecular methods, including Diaporthe eres, D. infecunda, Eutypella citricola, E. microtheca, Phaeoacremonium parasiticum, P. scolyti, P. australiense, P. minimum, Fomitiporia capensis, Fomitiporia sp., Fomitiporella sp., and Inocutis sp., which were isolated from persimmon for the first time in the world. Other first reports from persimmon in South Africa include D. foeniculina, D. ambigua, D. mutila, Diaporthe sp., Neofusicoccum australe, N. parvum, Diplodia seriata, and Eutypa lata. Pathogenicity tests conducted with all species, except the basidiomycetes, confirmed their status as possible persimmon pathogens. This is the first study to determine and identify fungi associated with diseased persimmon in South Africa. The knowledge gained in this study forms the basis for further research to determine the impact of these fungi on persimmon productivity.
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Úrbez-Torres, J. R., F. Peduto, P. M. Vossen, W. H. Krueger, and W. D. Gubler. "Olive Twig and Branch Dieback: Etiology, Incidence, and Distribution in California." Plant Disease 97, no. 2 (February 2013): 231–44. http://dx.doi.org/10.1094/pdis-04-12-0390-re.
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Eighteen different fungal species were isolated from symptomatic wood of olive trees (Olea europaea) affected by twig and branch dieback in California and identified by means of morphological characters and multigene sequence analyses of the internal transcribed spacer (ITS) region (ITS1-5.8S-ITS2), a partial sequence of the β-tubulin gene, and part of the translation elongation factor 1-α gene (EF1-α). These species included Diaporthe viticola, Diatrype oregonensis, Diatrype stigma, Diplodia mutila, Dothiorella iberica, Lasiodiplodia theobromae, Phaeomoniella chlamydospora, Phomopsis sp. group 1, Phomopsis sp. group 2, and Schizophyllum commune, which are for the first time reported to occur in olive trees; Eutypa lata, Neofusicoccum luteum, Neofusicoccum vitifusiforme, and Phaeoacremonium aleophilum, which are for the first time reported to occur in olive trees in the United States; and Botryosphaeria dothidea, Diplodia seriata, Neofusicoccum mediterraneum, and Trametes versicolor, which have been previously reported in olive trees in California. Pathogenicity studies conducted in olive cultivars Manzanillo and Sevillano showed N. mediterraneum and Diplodia mutila to be the most virulent species and Diatrype stigma and D. oregonensis the least virulent when inoculated in olive branches. Intermediate virulence was shown for the rest of the taxa. This study demystifies the cause of olive twig and branch dieback and elucidates most of the fungal pathogens responsible for this disease in California.
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Shigo, Alex L. "How tree branches are attached to trunks." Canadian Journal of Botany 63, no. 8 (August 1, 1985): 1391–401. http://dx.doi.org/10.1139/b85-193.
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The vascular cambium and the growth ring it produces are continuous from trunk to branch, but the cells formed by the cambium in the upper junction of branch and trunk are oriented at approximately right angles to the normal orientation in the trunk and branch. Branch tissues begin to develop before trunk tissues early in the growing season. Maturation of branch tissues proceeds basipetally. The branch xylem is oriented downward at the branch base and encircles it to form a collar. The collar tissues meet on the trunk below the branch. The branch collar is enveloped later in the growing season by a collar of trunk xylem. Xylem in the trunk collar meet above and below the branch. Conduction into and out of the branch follows the pathway of the branch collar. The branch is structurally attached to the trunk by a series of trunk collars that envelop the branch collars every growing season. When the trunk collar was injured or removed by branch pruning, the trunk xylem above and below the cut was rapidly and extensively infected and decay developed. When pruning cuts did not injure or remove the trunk collar, no infections developed in the trunk xylem. Dye movement and the patterns of spread of bacterial and fungal pathogens also suggested that there was no local direct conduction between trunk xylem above a branch and within a branch.
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Gramaje, D., and J. Armengol. "Fungal Trunk Pathogens in the Grapevine Propagation Process: Potential Inoculum Sources, Detection, Identification, and Management Strategies." Plant Disease 95, no. 9 (September 2011): 1040–55. http://dx.doi.org/10.1094/pdis-01-11-0025.
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Luque, J., G. Elena, F. Garcia-Figueres, J. Reyes, G. Barrios, and F. J. Legorburu. "Natural infections of pruning wounds by fungal trunk pathogens in mature grapevines in Catalonia (Northeast Spain)." Australian Journal of Grape and Wine Research 20, no. 1 (September 22, 2013): 134–43. http://dx.doi.org/10.1111/ajgw.12046.
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Richards, Andrew, Mehrbod Estaki, José Ramón Úrbez-Torres, Pat Bowen, Tom Lowery, and Miranda Hart. "Cover Crop Diversity as a Tool to Mitigate Vine Decline and Reduce Pathogens in Vineyard Soils." Diversity 12, no. 4 (March 30, 2020): 128. http://dx.doi.org/10.3390/d12040128.
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Wine grape production is an important economic asset in many nations; however, a significant proportion of vines succumb to grapevine trunk pathogens, reducing yields and causing economic losses. Cover crops, plants that are grown in addition to main crops in order to maintain and enhance soil composition, may also serve as a line of defense against these fungal pathogens by producing volatile root exudates and/or harboring suppressive microbes. We tested whether cover crop diversity reduced disease symptoms and pathogen abundance. In two greenhouse experiments, we inoculated soil with a 106 conidia suspension of Ilyonectria liriodendri, a pathogenic fungus, then conditioned soil with cover crops for several months to investigate changes in pathogen abundance and fungal communities. After removal of cover crops, Chardonnay cuttings were grown in the same soil to assess disease symptoms. When grown alone, white mustard was the only cover crop associated with reductions in necrotic root damage and abundance of Ilyonectria. The suppressive effects of white mustard largely disappeared when paired with other cover crops. In this study, plant identity was more important than diversity when controlling for fungal pathogens in vineyards. This research aligns with other literature describing the suppressive potential of white mustard in vineyards.
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Saremi, Hossein, Mohammad E. Amiri, and M. Mirabolfathi. "Application of Soil Solarization for Controlling Soilborne Fungal Pathogens in Newly Established Pistachio and Olive Orchards." International Journal of Fruit Science 10, no. 2 (June 11, 2010): 143–56. http://dx.doi.org/10.1080/15538362.2010.492332.
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Bonanomi, Giuliano, Veronica Giorgi, Del Sorbo Giovanni, Davide Neri, and Felice Scala. "Olive mill residues affect saprophytic growth and disease incidence of foliar and soilborne plant fungal pathogens." Agriculture, Ecosystems & Environment 115, no. 1-4 (July 2006): 194–200. http://dx.doi.org/10.1016/j.agee.2006.01.002.
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Li, Hui, Siva L. S. Velivelli, and Dilip M. Shah. "Antifungal Potency and Modes of Action of a Novel Olive Tree Defensin Against Closely Related Ascomycete Fungal Pathogens." Molecular Plant-Microbe Interactions® 32, no. 12 (December 2019): 1649–64. http://dx.doi.org/10.1094/mpmi-08-19-0224-r.
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Antimicrobial peptides play a pivotal role in the innate immunity of plants. Defensins are cysteine-rich antifungal peptides with multiple modes of action. A novel Oleaceae-specific defensin gene family has been discovered in the genome sequences of wild and cultivated species of a perennial olive tree, Olea europaea. OefDef1.1, a member of this defensin family, potently inhibits the in-vitro growth of ascomycete fungal pathogens Botrytis cinerea and three Fusarium spp. OefDef1.1 rapidly permeabilizes the plasma membrane of the conidial and germling cells of B. cinerea. Interestingly, it induces reactive oxygen species and translocates to the cytoplasm only in the germlings but not in the conidia. In medium containing a high concentration of Na1+, antifungal activity of OefDef1.1 is significantly reduced. Surprisingly, a chimeric OefDef1.1 peptide containing the γ-core motif of a Medicago truncatula defensin, MtDef4, displays Na1+-tolerant antifungal activity. In a phospholipid-protein overlay assay, the chimeric peptide exhibits stronger binding to its phosphoinositide partners than OefDef1.1 and is also more potent in inhibiting gray mold disease on the surface of Nicotiana benthamiana and lettuce leaves than OefDef1.1. Significant differences are observed among the four ascomycete pathogens in their responses to OefDef1.1 in growth medium with or without the elevated concentration of Na1+. The varied responses of closely related ascomycete pathogens to this defensin have implications for engineering disease resistance in plants.
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Chacón-Vozmediano, Juan L., David Gramaje, Maela León, Josep Armengol, Juan Moral, Pedro M. Izquierdo-Cañas, and Jesús Martínez-Gascueña. "Cultivar Susceptibility to Natural Infections Caused by Fungal Grapevine Trunk Pathogens in La Mancha Designation of Origin (Spain)." Plants 10, no. 6 (June 9, 2021): 1171. http://dx.doi.org/10.3390/plants10061171.
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Grapevine trunk diseases (GTDs) are one of the main biotic stress factors affecting this crop. The use of tolerant grapevine cultivars would be an interesting and sustainable alternative strategy to control GTDs. To date, most studies about cultivar susceptibility have been conducted under controlled conditions, and little information is available about tolerance to natural infections caused by GTD fungi. The objectives of this study were: (i) to identify tolerant cultivars to GTD fungi within a Spanish germplasm collection, based on external symptoms observed in the vineyard; and (ii) to characterize the pathogenic mycoflora associated with symptomatic vines. For this purpose, a grapevine germplasm collection including 22 white and 25 red cultivars was monitored along three growing seasons, and their susceptibility for esca foliar symptoms was assessed. Fungi were identified by using morphological and molecular methods. Cultivars such as, ‘Monastrell’, ‘Graciano’, ‘Cabernet Franc’, ‘Cabernet Sauvignon’, ‘Syrah’, ‘Moscatel de Alejandría’, ‘Sauvignon Blanc’, and ‘Airén’ displayed high susceptibility to GTDs, whereas others such as ‘Petit Verdot’, ‘Pinot Noir’, ‘Chardonnay’, and ‘Riesling’ were considered as tolerant. The prevalent fungal species isolated from symptomatic vines were Phaeomoniella chlamydospora (27.9% of the fungal isolates), Cryptovalsa ampelina (24.6%), and Dothiorella sarmentorum (21.3%).
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Úrbez-Torres, J. R., P. Haag, P. Bowen, and D. T. O'Gorman. "Grapevine Trunk Diseases in British Columbia: Incidence and Characterization of the Fungal Pathogens Associated with Black Foot Disease of Grapevine." Plant Disease 98, no. 4 (April 2014): 456–68. http://dx.doi.org/10.1094/pdis-05-13-0524-re.
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Black foot disease of grapevines, caused by several fungal species in the genera Campylocarpon, Cylindrocarpon, Cylindrocladiella, and Ilyonectria, causes significant economic losses to the grapevine industry worldwide. This study represents the first attempt to identify and characterize the fungal pathogens associated with black foot disease of grapevines in British Columbia (BC). Field surveys conducted throughout all grape-growing regions in BC that included assessment of foliar symptomatology and isolations from symptomatic vines showed Cylindrocarpon/Ilyonectria spp. occurred in 32 of 90 (35.5%) young vineyards surveyed (≤8 year old) and in 41 of 215 (19%) samples collected. In 20 of the 41 (48.8%) samples, Cylindrocarpon/Ilyonectria spp. were the sole fungi isolated from symptomatic tissue. In the rest of the samples, black foot fungi were found to primarily coexist with fungal taxa associated with Petri disease of grapevines. Colony and conidia phenotypical characterization, along with DNA analyses of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rDNA, and part of the β-tubulin and translation elongation factor 1-α genes, revealed five different black foot fungi occurring in declining young vines in BC, namely Cylindrocarpon pauciseptatum, Ilyonectria liriodendri, Ilyonectria macrodidyma, Ilyonectria robusta, and Ilyonectria torresensis. Pathogenicity studies showed all five species to be highly virulent in the grapevine rootstock cultivar 3309C. Overall, I. liriodendri and I. macrodidyma were the most virulent species when inoculated in Vitis vinifera ‘Chardonnay’ and rootstock 3309C.
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Lima, G., D. Piedimonte, F. De Curtis, A. Abobaker Elgelane, F. Nigro, A. M. D'Onghia, G. Alfano, and G. Ranalli. "SUPPRESSIVE EFFECT OF CURED COMPOST FROM OLIVE OIL BY-PRODUCTS TOWARDS VERTICILLIUM DAHLIAE AND OTHER FUNGAL PATHOGENS." Acta Horticulturae, no. 791 (June 2008): 585–91. http://dx.doi.org/10.17660/actahortic.2008.791.90.
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Pečenka, Jakub, Eliška Peňázová, Dorota Tekielska, Ivo Ondrášek, Tomáš Nečas, and Aleš Eichmeier. "Fungi detected in trunk of stone fruits in the Czech Republic." Acta Agraria Debreceniensis, no. 72 (February 8, 2019): 121–27. http://dx.doi.org/10.34101/actaagrar/72/1602.
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This study was focused on detection of the spectrum of fungi in the wood of stone fruits using molecular genetic methods. Samples were obtained from apricots, plums and sweet cherry trees from region of Moravia, one sample was obtained from Myjava (Slovakia). Segments of symptomatic wood were obtained from dying stone fruit trees with very significant symptoms. This study describes detection of the fungi in the wood of 11 trees in general in 5 localities. The cultivation of the fungi from symptomatic wood and sequencing of ITS was carried out. Eleven fungal genera were determined in the stone fruits wood, particularly Irpex lacteus, Fomes fomentarius, Neofabraea corticola, Calosphaeria pulchella, Cytospora leucostoma, Phellinus tuberculosus, Stereum hirsutum, Collophora sp., Pithomyces chartarum, Aureobasidium pullulans,Fusarium sp. The results of this study demonstrate that the reason of declining of stone fruit trees in Moravia is caused probably by trunk pathogens.
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López‐Moral, Ana, María Carmen Raya, Cristina Ruiz‐Blancas, Ignacio Medialdea, María Lovera, Octavio Arquero, Antonio Trapero, and Carlos Agustí‐Brisach. "Aetiology of branch dieback, panicle and shoot blight of pistachio associated with fungal trunk pathogens in southern Spain." Plant Pathology 69, no. 7 (June 8, 2020): 1237–69. http://dx.doi.org/10.1111/ppa.13209.
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Buzón-Durán, Laura, Natalia Langa-Lomba, Vicente González-García, José Casanova-Gascón, Jesús Martín-Gil, Eduardo Pérez-Lebeña, and Pablo Martín-Ramos. "On the Applicability of Chitosan Oligomers-Amino Acid Conjugate Complexes as Eco-Friendly Fungicides against Grapevine Trunk Pathogens." Agronomy 11, no. 2 (February 12, 2021): 324. http://dx.doi.org/10.3390/agronomy11020324.
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In a context in which the incidence and severity of grapevine fungal diseases is increasing as a result of both climate change and modern management culture practices, reducing the excessive use of phytosanitary products in viticulture represents a major challenge. Specifically, grapevine trunk diseases (GTDs), caused by several complexes of wood decay or xylem-inhabiting fungi, pose a major challenge to vineyard sustainability. In this study, the efficacy of chitosan oligomers (COS)–amino acid conjugate complexes against three fungal species belonging to the Botryosphaeriaceae family (Neofusicoccum parvum, Diplodia seriata, and Botryosphaeria dothidea) was investigated both in vitro and in planta. In vitro tests led to EC50 and EC90 effective concentrations in the 254.6−448.5 and 672.1−1498.5 µg·mL−1 range, respectively, depending on the amino acid involved in the conjugate complex (viz. cysteine, glycine, proline or tyrosine) and on the pathogen assayed. A synergistic effect between COS and the amino acids was observed against D. seriata and B. dothidea (synergy factors of up to 2.5 and 2.8, respectively, according to Wadley’s method). The formulations based on COS and on the conjugate complex that showed the best inhibition rates, COS−tyrosine, were further investigated in a greenhouse trial on grafted vines of two varieties (”Tempranillo” on 775P and “Garnacha” on 110R rootstock), artificially inoculated with the mentioned three Botryosphaeriaceae species. The in planta bioassay revealed that the chosen formulations induced a significant decrease in disease severity against N. parvum and B. dothidea. In summary, the reported conjugate complexes may be promising enough to be worthy of additional examination in larger field trials.
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Marra, Roberta, Mariangela Coppola, Angela Pironti, Filomena Grasso, Nadia Lombardi, Giada d’Errico, Andrea Sicari, et al. "The Application of Trichoderma Strains or Metabolites Alters the Olive Leaf Metabolome and the Expression of Defense-Related Genes." Journal of Fungi 6, no. 4 (December 16, 2020): 369. http://dx.doi.org/10.3390/jof6040369.
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Biocontrol fungal strains of the genus Trichoderma can antagonize numerous plant pathogens and promote plant growth using different mechanisms of action, including the production of secondary metabolites (SMs). In this work we analyzed the effects of repeated applications of selected Trichoderma strains or SMs on young olive trees on the stimulation of plant growth and on the development of olive leaf spot disease caused by Fusicladium oleagineum. In addition, metabolomic analyses and gene expression profiles of olive leaves were carried out by LC–MS Q-TOF and real-time RT-PCR, respectively. A total of 104 phenolic compounds were detected from olive leave extracts and 20 were putatively identified. Targeted and untargeted approaches revealed significant differences in both the number and type of phenolic compounds accumulated in olive leaves after Trichoderma applications, as compared to water-treated plants. Different secoiridoids were less abundant in treated plants than in controls, while the accumulation of flavonoids (including luteolin and apigenin derivatives) increased following the application of specific Trichoderma strain. The induction of defense-related genes, and of genes involved in the synthesis of the secoiridoid oleuropein, was also analyzed and revealed a significant variation of gene expression according to the strain or metabolite applied.
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Berbegal, Mónica, Antonio Ramón‐Albalat, Maela León, and Josep Armengol. "Evaluation of long‐term protection from nursery to vineyard provided by Trichoderma atroviride SC1 against fungal grapevine trunk pathogens." Pest Management Science 76, no. 3 (October 4, 2019): 967–77. http://dx.doi.org/10.1002/ps.5605.
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Ghasemi-Sardareh, Rahil, and Hamid Mohammadi. "Characterization and pathogenicity of fungal trunk pathogens associated with declining of neem (Azadirachta indica A. Juss) trees in Iran." Journal of Plant Pathology 102, no. 4 (July 14, 2020): 1159–71. http://dx.doi.org/10.1007/s42161-020-00598-z.
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Gramaje, David, José Ramón Úrbez-Torres, and Mark R. Sosnowski. "Managing Grapevine Trunk Diseases With Respect to Etiology and Epidemiology: Current Strategies and Future Prospects." Plant Disease 102, no. 1 (January 2018): 12–39. http://dx.doi.org/10.1094/pdis-04-17-0512-fe.
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Fungal trunk diseases are some of the most destructive diseases of grapevine in all grape growing areas of the world. Management of GTDs has been intensively studied for decades with some great advances made in our understanding of the causal pathogens, their epidemiology, impact, and control. However, due to the breadth and complexity of the problem, no single effective control measure has been developed. Management of GTD must be holistic and integrated, with an interdisciplinary approach conducted in both nurseries and vineyards that integrates plant pathology, agronomy, viticulture, microbiology, epidemiology, biochemistry, physiology, and genetics. In this review, we identify a number of areas of future prospect for effective management of GTDs worldwide, which, if addressed, will provide a positive outlook on the longevity of vineyards in the future.
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Úrbez-Torres, J. R., F. Peduto, and W. D. Gubler. "First Report of Ilyonectria macrodidyma Causing Root Rot of Olive Trees (Olea europaea) in California." Plant Disease 96, no. 9 (September 2012): 1378. http://dx.doi.org/10.1094/pdis-04-12-0330-pdn.
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The California olive industry produces 99% of the U.S. olive crop, which represented a value of over $113 million in 2010. During the 2008 and 2009 growing seasons, decline of young super-high-density olive cvs. Arbequina, Arbosana, and Koroneiki trees (<4 years old) was observed in orchards throughout Glenn, Yolo, and San Joaquin Counties. Symptomatic trees showed stunted growth and chlorotic leaves with roots having black, sunken, necrotic lesions, which frequently prolonged into the base and crown of the tree. Twenty-five trees were collected from different orchards and necrotic roots as well as infected trunk tissue were plated onto potato dextrose agar amended with 0.01% tetracycline hydrochloride. Cultures were incubated at room temperature (23 ± 2°C) until fungal colonies were observed. In 17 out of 25 trees collected (68%), light yellow fungal colonies were observed from the symptomatic tissue after 7 to 10 days. Colonies turned dark yellow to orange with age and showed an orange-dark brown reverse. Both microconidia (hyaline, ellipsoidal to ovoidal and aseptate (n = 60) (6.5) 11.5 to 13.5 (17.1) × (3) 3.4 to 4.5 (5.6) μm) and macroconidia (hyaline, cylindrical, straight and/or slightly curved with one, two or three septa (n = 60) (12.5) 26.5 to 38.5 (44.1) × (4) 5.5 to 7.5 (8.5) μm) were observed. Culture and conidial morphology were in concordance with previous published description of Ilyonectria macrodidyma (Halleen, Schroers & Crous) P. Chaverri & C. Salgado (1,3,4). Identification to species level was confirmed by sequence comparison of four Californian isolates (UCCE958, UCCE959, UCCE960, and UCCE961) with sequences available in GenBank using the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rDNA (primers ITS1/ITS4), a portion of the β-tubulin gene (BT1a/BT1b), and a partial sequence of the mitochondrial small subunit rDNA (NMS1/NMS2) (4). Fungal sequences of isolates from olive from California (GenBank JQ868543 to JQ868554) showed 99 to 100% homology with previously identified and deposited I. macrodidyma isolates in Genbank for all three genes. Pathogenicity of I. macrodidyma in olive cvs. Arbequina, Arbosan, and Koroneiki was investigated using two fungal isolates (UCCE958 and UCCE960) as reported by Petit and Gubler (4). The roots of 10 1-year-old trees per fungal isolate for each olive cultivar were individually inoculated with 25 ml of a 106 conidia/ml spore suspension and placed in a lath house at the UC Davis field station. Additionally, 10 trees per cultivar were inoculated with sterile water as controls. Six months after inoculation, most of the inoculated olive plants showed chlorotic leaves similar to those observed in commercial orchards. Root necrosis for each cv. was expressed as the percentage of root length having lesions (2). No significant difference was observed between isolates and average root necrosis was 29.4, 35.6, and 38.3% in Koroniki, Arbosana, and Arbequina, respectiveley. I. macrodidyma was recovered from symptomatic roots in each of the cvs. and identified based on morphology. No root rot symptoms were observed in the controls. To our knowledge, this is the first report of I. macrodidyma causing root rot of olive trees not only in California but anywhere in the world. References: (1) P. Chaverri et al. Stud. Mycol. 68:57, 2011. (2) M. Giovanetti and B. Mosse. New Phytol. 84:489, 1980. (3) F. Halleen et al. Stud. Mycol. 50:421, 2004. (4) E. Petit and W. D. Gubler. Plant Dis. 89:1051, 2005.
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Alonso, Raquel, Susana Tiscornia, Acelino Couto Alfenas, and Lina Bettucci. "Fungi associated to bark lesions of Eucalyptus globulus stems in plantations from Uruguay." Revista Árvore 33, no. 4 (August 2009): 591–97. http://dx.doi.org/10.1590/s0100-67622009000400001.
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Trees with stem bark lesions are frequently observed in Eucalyptus globulus Labill. plantations, particularly in the central west region of Uruguay. These lesions constitute a problem for trunk decortications at harvest and they also facilitate the access of fungi that could cause wood decay. Seven, three and oneyear-old plantations, located at three sites in close proximity were selected. Four types of trunk lesions were present in trees regardless the age of plantation and more than one type was found in each plantation. The aim of this study was to investigate the fungal composition associated with these lesions and compare them to healthy tissues and try to find out the origin of these symptoms. Another purpose was to elucidate the real role of the fungi considered pathogens by means of experimental inoculations. Segments from lesions and healthy tissues yielded 897 fungal isolates belonging to 32 taxa, 681 isolates from bark lesions and 216 from healthy tissues. Both healthy and symptomatic tissues showed similar fungal species composition, but with differences in frequencies of colonization. Cytospora eucalypticola Van der Westhuizen, Botryosphaeria spp., Pestalotiopsis guepinii (Desm.) Stey. and Penicillium spp. were the dominant species isolated. As symptoms were not reproduced after experimental inoculation with Botryosphaeria ribis Grossenb. & Duggar and B. eucalyptorum Crous, & M.J. Wingf, it could be suggested that these lesions were originated by unfavorable environmental conditions. The frost that occurred for several days out of season and flooding may have been involved in the development of bark lesion.
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Del Frari, Giovanni, Helena Oliveira, and Ricardo Boavida Ferreira. "White Rot Fungi (Hymenochaetales) and Esca of Grapevine: Insights from Recent Microbiome Studies." Journal of Fungi 7, no. 9 (September 17, 2021): 770. http://dx.doi.org/10.3390/jof7090770.
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Esca is a major grapevine trunk disease that heavily affects vineyards in the Northern hemisphere. The etiology and epidemiology of this disease have been subject of dispute ever since the earliest disease reports. The reason behind such debate is the presence of multiple internal and external symptoms, as well as several putative and confirmed wood pathogens. While the role of pathogenic fungi, as causal agents of wood symptoms, has been thoroughly assessed, their role in the expression of leaf symptoms remains to be fully elucidated. In this review, we analyzed etiological and epidemiological data, with a special focus on the microbiological aspect of esca and the involvement of Hymenochaetales (Basidiomycota). Vineyard studies have associated leaf symptoms with the presence of white rot, most frequently caused by Fomitiporia mediterranea (Hymenochaetales), while tracheomycotic fungi are commonly found, with similar abundance, in symptomatic and asymptomatic vines. Pathogenicity trials have excluded a direct effect of Hymenochaetales species in triggering leaf symptoms, while the data concerning the role of tracheomycotic fungi remains controversial. Recent microbiome studies confirmed that F. mediterranea is more abundant in leaf-symptomatic vines, and treatments that effectively control leaf symptoms, such as sodium arsenite spray and trunk surgery, act directly on the abundance of F. mediterranea or on the presence of white rot. This suggest that the simultaneous presence of Hymenochaetales and tracheomycotic fungi is a pre-requisite for leaf symptoms; however, the relation among fungal pathogens, grapevine and other biotic and abiotic factors needs further investigation.
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Úrbez-Torres, J. R., P. Haag, P. Bowen, and D. T. O'Gorman. "Grapevine Trunk Diseases in British Columbia: Incidence and Characterization of the Fungal Pathogens Associated with Esca and Petri Diseases of Grapevine." Plant Disease 98, no. 4 (April 2014): 469–82. http://dx.doi.org/10.1094/pdis-05-13-0523-re.
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Esca and Petri disease are two economically important grapevine diseases worldwide. This study reports for the first time the occurrence of both diseases on grapevines in British Columbia (BC) and subsequently in Canada. Visual assessment of 55,699 vines in 118 vineyards revealed a low incidence of esca with only 104 (0.2%) vines showing foliar symptoms. Young vine decline (YVD) was observed in 1,910 (7.8%) of 24,487 monitored young vines and in 52 (8%) of 654 young vines used as re-plants in mature vineyards. In 8 of 51 monitored young vineyards, YVD-affected vines ranged between 15 and 55%. Morphological studies along with DNA analyses of the ITS1-5.8S-ITS2, and part of the β-tubulin, actin, and translation elongation factor 1-α gene regions, allowed us to identify Cadophora luteoolivacea, Phaeomoniella chlamydospora, Phaeoacremonium iranianum, Togninia fraxinopennsylvanica, Togninia minima, and the novel species Phaeoacremonium canadense and Phaeoacremonium roseum from esca and Petri disease infected vines in BC. This study includes for the first time the EF1-α DNA marker in Phaeoacremonium spp. delineation. Pathogenicity studies showed all seven fungi to cause vascular symptoms similar to those observed in esca and Petri disease infected vines. Additionally, the “tiger-stripes” foliar symptom of esca was successfully reproduced when healthy potted vines were inoculated with BC isolates of Pa. chlamydospora, Pm. canadense, Pm. iranianum, T. fraxinopennsylvanica, and T. minima.