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Dissertations / Theses on the topic 'Odorant Binding Proteins'

Author: Grafiati

Published: 4 June 2021

Last updated: 18 May 2024

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1

Jacobs, Stephen P. "Chemosensory proteins and odorant binding proteins in aphids." Thesis, University of Nottingham, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.435766.

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2

Cheng, Hui-Yin Patricia. "Towards microarrays of fluorescent odorant binding proteins." Thesis, Imperial College London, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.509508.

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3

Tuccori, Elena. "Development of biosensors based on Odorant Binding Proteins." Thesis, University of Manchester, 2014. https://www.research.manchester.ac.uk/portal/en/theses/development-of-biosensors-based-on-odorant-binding-proteins(7ce472da-bfbf-4fb0-a0bc-ed61d0d3be49).html.

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This PhD project aimed to investigate the possibility of using Odorant Binding Proteins (OBPs) as sensing layers of chemical sensors, for the detection of organic compounds in both vapour and liquid phases. OBPs are small soluble proteins present in high concentrations in the olfactory system of vertebrates and insects. OBPs are attractive in the biosensor field since they can bind odorants and pheromones in a reversible way. They are resistant to high temperatures and protease activity and they can be easily expressed in large amounts. OBPs belonging to different species of mammals and insect

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4

Rihani, Karen. "Role of odorant-binding proteins in Drosophila melanogaster chemosensory perception." Thesis, Bourgogne Franche-Comté, 2019. http://www.theses.fr/2019UBFCK044.

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La perception des signaux chimiques de l’environnement est un processus nécessaire aux interactions sociales entre les animaux. La Drosophile détecte les molécules odorantes et sapides grâce à ses systèmes gustatif et olfactif impliquant plusieurs familles multigèniques de chimiorécepteurs. Ainsi, ces composés chimiques pénétrant dans l'organe sensoriel (sensille) doivent être solubilisés avant d'être transportés à travers la lymphe sensillaire hydrophile baignant les dendrites des neurones chimiosensoriels. Ces événements périrecepteurs font intervenir plusieurs familles de protéines solubles

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5

Agnihotri, Aniruddha Ravindra. "Molecular study of odorant binding proteins to better understand insect chemosensation." Thesis, Agnihotri, Aniruddha Ravindra (2021) Molecular study of odorant binding proteins to better understand insect chemosensation. PhD thesis, Murdoch University, 2021. https://researchrepository.murdoch.edu.au/id/eprint/65502/.

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Like many other organisms, insects use chemical stimuli to regulate behaviours including feeding, egg-laying, and mating. Odorant binding proteins (OBPs) are one of the crucial components of insect chemosensory system, which play an essential role in transporting the hydrophobic volatile odorant molecules to the olfactory receptors. Most of insect OBPs were investigated by using recombinant technology in bacterial cells due to its fast, cost-effective, and high production mechanism. However, one of the major concerns of bacterial expression system is that the protein is frequently expressed in

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6

Foret, Sylvain, and sylvain foret@anu edu au. "Function and Evolution of Putative Odorant Carriers in the Honey Bee (Apis mellifera)." The Australian National University. Research School of Biological Sciences, 2007. http://thesis.anu.edu.au./public/adt-ANU20070613.144745.

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The remarkable olfactory power of insect species is thought to be generated by a combinatorial action of G-protein-coupled olfactory receptors (ORs) and olfactory carriers. Two such carrier gene families are found in insects: the odorant binding proteins (OBPs) and the chemosensory proteins (CSPs). In olfactory sensilla, OBPs and CSPs are believed to deliver hydrophobic air-borne molecules to ORs, but their expression in non-olfactory tissues suggests that they also may function as general carriers in other developmental and physiological processes. ¶ Bioinformatics and experimental approac

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7

Maïbèche-Coisné, Martine. "Etudes structurale et fonctionnelle des odorant-binding proteins chez la noctuelle mamestra brassicae l. (lepidoptera : noctuidea)." Paris 6, 1997. http://www.theses.fr/1997PA066126.

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L'olfaction est un des modes sensoriels predominant chez les lepidopteres crepusculaires et nocturnes, et ces animaux s'averent un modele de choix pour l'etude des mecanismes moleculaires de la reception des signaux olfactifs et de leur transduction vers des niveaux superieurs d'integration. Depuis leur decouverte dans le systeme olfactif peripherique des insectes et des vertebres, le degre d'implication des odorant-binding proteins (obp) dans les processus de discrimination des odeurs n'est toujours pas elucide. Dans ce cadre, nous avons entrepris l'etude de telles proteines chez la noctuelle

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8

Manoharan, Malini. "Genomic, structural and functional characterization of odorant binding proteins in olfaction of mosquitoes involved in infectious disease transmission." Phd thesis, Université de la Réunion, 2011. http://tel.archives-ouvertes.fr/tel-00979587.

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The role of odorant binding proteins in the olfaction of mosquitoes, the primary mechanism of human host recognition, has been an important focus of biological research in the field of infectious disease transmission by these insects. This thesis provides an in depth knowledge of these proteins in three mosquito species Anopheles gambiae, Aedes aegypti and Culex quinquefasciatus. A large scale analysis on these genomes has been carried out towards the identification of the odorant binding proteins in the mosquito genomes. Identification of many new OBP members, in particular in the Aedes aegyp

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9

Rojas, Gallardo Diana Marcela. "Evolução molecular da família gênica dos receptores de odores e proteínas ligantes a feromônios e genética de populações de genes quimiossensoriais em espécies de Anastrepha do grupo fraterculus." Universidade Federal de São Carlos, 2016. https://repositorio.ufscar.br/handle/ufscar/8773.

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Submitted by Alison Vanceto (alison-vanceto@hotmail.com) on 2017-05-22T12:42:04Z No. of bitstreams: 1 DissDMRG.pdf: 6251788 bytes, checksum: 7ef11e629010aba06934d05d8113712d (MD5)<br>Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-05-23T20:34:35Z (GMT) No. of bitstreams: 1 DissDMRG.pdf: 6251788 bytes, checksum: 7ef11e629010aba06934d05d8113712d (MD5)<br>Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-05-23T20:34:41Z (GMT) No. of bitstreams: 1 DissDMRG.pdf: 6251788 bytes, checksum: 7ef11e629010aba06934d05d8113712d (MD5)<br>Made availabl

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10

Bunyarataphan, Sasinee. "Biosensors based on bovine odorant binding protein (bOBP)." Thesis, Imperial College London, 2013. http://hdl.handle.net/10044/1/11053.

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Recombinant bovine odorant binding protein (bOBP) is a very promising platform for building protein-based biosensors. The protein possesses a broad binding specificity for hydrophobic molecules with affinities in the sub-micromolar range. Previous work has shown non-covalent binding of 1-aminoanthracene (1-AMA) in the internal cavities of bOBP that results in a large enhancement of fluorescence intensity. We have shown fluorescence titrations of recombinant bOBP with 1-AMA yielded a single type of binding site with a Kd of 0.16 ± 0.023 μM. Competitive displacement assays between 1-AMA and othe

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11

Bengtsson, Linda. "Odorant binding protein and olfactory receptors: plausible role as detectors in an odorant biosensor." Thesis, KTH, Skolan för kemivetenskap (CHE), 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-32897.

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The development of an inexpensive, portable, stable, sensitive and selective biosensor for detection of odorants is a daunting task. Here, we hypothesized the development of a detector layer composed of the protein groups; the olfactory receptors (ORs) and the odorant binding proteins (OBPs), known to bind odorants in animal sensing. We report the design of 13 OR gene-vector constructs, and their subsequent transformation into Escherichia (E.) coli (BL21 (DE3)-STAR-pLysS) strain. Moreover, we report the expression of several ORs into an in vitro wheat germ extract using three separate detergen

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12

Thode, Anna Begnaud. "Investigating protein-alcohol interactions in the Drosophila melanogaster protein LUSH /." Connect to abstract via ProQuest. Full text is not available online, 2007.

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Thesis (Ph.D. in Biochemistry, Biomolecular Structure Program) -- University of Colorado Denver, 2007.<br>Typescript. Includes bibliographical references (leaves 135-146). Online version available via ProQuest Digital Dissertations.

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13

Sengul, Meryem Senay. "Two Odorant-Binding Protein Genes in Mosquitoes: Comparative Genomics, Expression, and Function." Diss., Virginia Tech, 2008. http://hdl.handle.net/10919/26578.

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Insect Odorant-Binding Proteins (OBPs) are small, water-soluble molecules that solubilize hydrophobic odorant molecules in the sensillum lymph and transport them to their cognate receptors in the olfactory receptor neurons. With the availability of the genome sequence of the African malaria mosquito, Anopheles gambiae, there has been a profound interest in the characterization and functional analyses of Obp genes in order to understand the molecular basis of mosquito host-seeking behavior. However, no direct evidence has been found for specific functions of any mosquito OBPs. In this study,

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14

El, kazzy Marielle. "Etude fondamentale pour l'optimisation des performances d'un nez bioélectronique basé sur des protéines liant les odorants." Electronic Thesis or Diss., Université Grenoble Alpes, 2023. http://www.theses.fr/2023GRALV105.

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La détection de molécules odorantes et de composés organiques volatils (COVs) fait l'objet d'une demande croissante dans divers domaines tels que l’industrie alimentaire, la parfumerie, le diagnostic médical, la surveillance environnementale, etc. Bien que précises et fiables, les méthodes les plus couramment employées - la chromatographie en phase gazeuse couplée à la spectrométrie de masse et les panels de nez humain ou encore des nez de chiens dressés- présentent un certain nombre d'inconvénients notamment en termes de coût et de temps. En réponse à ces contraintes, les nez électroniques (N

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15

Brimau, Fanny. "Rôle des odorants-binding protein dans le mécanisme de transduction olfactive : implication de modifications post-traductionnelles dynamiques dans la spécificité de liaison avec les ligands." Thesis, Tours, 2010. http://www.theses.fr/2010TOUR4038/document.

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Les OBP sont des petites protéines solubles qui se lient avec des molécules odorantes et phéromonales. Le rôle des OBPs n’est pas complètement compris. Une hypothèse suggère que l’OBP solubilise et transporte les ligands aux récepteurs olfactifs et la liaison entre les molécules odorantes et l’OBP est non spécifique. Une autre hypothèse suggère que le complexe formé est une liaison spécifique entre une molécule odorante donnée et une OBP spécifique. Ce travail de thèse montre que les OBPs sont impliquées dans la première étape de la discrimination des odeurs. Dans un premier temps, nous avons

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16

Carvalho, Josiane Aparecida de. "Lipocalina bovina e o seu papel na resistência ao carrapato: quantificação em líquidos corporais de raças bovinas com fenótipos contrastantes de infestações com o carrapato Rhipicephalus microplus." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/17/17147/tde-06062017-161955/.

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Rhipicephalus micropulus, conhecido como carrapato dos bovinos, é um dos parasitas mais importantes para a pecuária, pois causa enormes prejuízos ao produtor. Esses prejuízos se estendem desde a ação espoliativa que o carrapato exerce nos seus hospedeiros até a transmissão de uma diversidade de patógenos. Os carrapatos utilizam vários estímulos térmicos, sonoros, visuais, gustativos, táteis e olfativos na fase de busca pelo hospedeiro. Essas substâncias são conhecidas como semioquímicos e podem atuar em indivíduos de uma mesma espécie como os ferormônios ou entre indivíduos de espécies diferen

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17

Santana, Isis Bugia. "Modelagem comparativa e triagem virtual hier?rquica para identifica??o de moduladores das OBPs de Lutzomyia Longipalpis." Universidade Estadual de Feira de Santana, 2016. http://localhost:8080/tede/handle/tede/441.

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Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-02-14T00:41:33Z No. of bitstreams: 1 PPGBiotec - Disserta??o corrigida - Isis Bugia.pdf: 6811384 bytes, checksum: 2380cbb790d35324858de90e106415fc (MD5)<br>Made available in DSpace on 2017-02-14T00:41:33Z (GMT). No. of bitstreams: 1 PPGBiotec - Disserta??o corrigida - Isis Bugia.pdf: 6811384 bytes, checksum: 2380cbb790d35324858de90e106415fc (MD5) Previous issue date: 2016-03-11<br>The Visceral Leishmaniasis (VL) is the second most important vector-borne disease in the world, transmitted in the Americas by Lu

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18

Millery, Julie. "Une adaptation du système olfactif au milieu aérien : les Olfactory-Binding Protein (OBP) chez Xenopus laevis et Xenopus tropicalis." Dijon, 2009. http://www.theses.fr/2009DIJOS047.

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Les Olfactory-binding protein (OBP), sont des protéines largement retrouvées dans le mucus olfactif d’un grand nombre de vertébrés mais elles n’ont cependant jamais été mises en évidence chez le poisson. Assez peu de choses sont connues sur leur fonction exacte au sein du système olfactif mais un rôle dans l’adaptation de ce système au milieu aérien est fortement suspecté. Le xénope possède un double système olfactif composé de deux cavités l’une dédiée à l’olfaction aquatique (CM) et l’autre dédiée à l’olfaction aérienne (CP). Cette particularité nous a permis de tester notre hypothèse. Nous

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19

Watt, William C. "Neuroplasticity in olfactory sensation /." Thesis, Connect to this title online; UW restricted, 2003. http://hdl.handle.net/1773/6252.

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20

FARINA, DONATELLA. "Research and development of innovative molecular sensor technologies for the monitoring of volatile organic compounds in wines." Doctoral thesis, Università degli studi della Basilicata, 2021. http://hdl.handle.net/11563/146584.

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The scientific and social increasing demand for quantitative/qualitative determination of natural and artificial chemical species in different sector of human life (health, living environment, food, etc.) highlights the need for new strategies and new measurement methods. In particular, the agro-food industries need quick and sensitive tools to identify molecules in its final products or to monitor them during production processes. This need is due to the increase of food frauds. Indeed, in last decades, adulteration and falsification of food become a very serious problems also as regard human

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21

Cann, Paul. "Étude des mécanismes de réception des phéromones mâles chez les petits ruminants." Thesis, Lille, 2020. http://www.theses.fr/2020LILUS115.

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Chez les petits ruminants (mouton et chèvre) la reproduction est saisonnée, c’est-à-dire qu’il y a alternance de période de repos et d’activité sexuelle. Ces deux périodes se caractérises chez les femelles par deux états physiologiques différents : en période d’activité sexuelle, le cycle ovarien des femelles est actif (oestrus) et la reproduction est possible, alors qu’en période de repos sexuel elles sont en anoestrus profond. Dans le but d’induire l’oestrus chez des femelles en repos sexuelle, les éleveurs ont le plus souvent recours à des hormones exogènes. Ces hormones sont de moins en mo

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22

Calvário, Joana Ramos dos Santos. "Production and Characterization of Odorant Binding Proteins." Master's thesis, 2022. http://hdl.handle.net/10362/133791.

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Odorant Binding Proteins (OBPs) are small soluble proteins responsible for the binding, solubilization and transportation of Volatile Organic Compounds (VOCs) to olfactory receptors in vertebrates and insects. These proteins have been gaining attraction in artificial olfaction fields due to their natural selectivity toward VOCs. Furthermore, OBPs are reported to be structurally stable, resistant to thermal and proteolytic degradation, and easily produced in E. coli. Therefore, it is possible to implement OBPs in biosensors in order to develop a sensor array with improved selectivity by mimicki

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23

Wang, Ping. "Functional evolution of odorant binding proteins genes in Drosophila melanogaster." 2009. http://www.lib.ncsu.edu/theses/available/etd-12182008-115702/unrestricted/etd.pdf.

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24

Foret, Sylvain. "Function and Evolution of Putative Odorant Carriers in the Honey Bee (Apis mellifera)." Phd thesis, 2006. http://hdl.handle.net/1885/45748.

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The remarkable olfactory power of insect species is thought to be generated by a combinatorial action of G-protein-coupled olfactory receptors (ORs) and olfactory carriers. Two such carrier gene families are found in insects: the odorant binding proteins (OBPs) and the chemosensory proteins (CSPs). In olfactory sensilla, OBPs and CSPs are believed to deliver hydrophobic air-borne molecules to ORs, but their expression in non-olfactory tissues suggests that they also may function as general carriers in other developmental and physiological processes. ¶ Bioinformatics and experimental approaches

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25

Vinkler, David. "Evoluce a exprese odoranty vázajících proteinů u vybraných zástupců rodu Mus." Master's thesis, 2011. http://www.nusl.cz/ntk/nusl-312772.

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Odorant-binding proteins (OBPs) are small soluble proteins expressed at high levels in the proximity of olfactory receptors. OBPs act as solubilizers and carriers of the lipophilic odorants in the aqueous mucus of mammals and other vertebrates. OBPs have now been studied nearly thirty years, but in comparison to the wealth of data available on their structural chemistry and molecular biology, our knowledge about gene expression and function of these proteins is still insufficient. This work provides new insights into the tissue specificity of OBP and presents several new sequences of genes gov

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26

Gonçalves, Filipa Daniela Gomes. "Liposome-OBP conjugates for odour reduction and fragance release." Doctoral thesis, 2021. http://hdl.handle.net/1822/75192.

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Tese de Doutoramento em Engenharia Química e Biológica<br>A atividade diária e o exercício físico são responsáveis pela produção de odores corporais desagradáveis que podem causar ansiedade e embaraço social. A procura de novas soluções que previnam o desenvolvimento desses odores é atualmente objeto de interesse para as indústrias da cosmética e têxtil. Nos mamíferos, as proteínas de ligação a odores (OBPs) são responsáveis pelo transporte de moléculas odoríferas do muco nasal aos recetores olfativos. As OBPs são proteínas extracelulares com uma estrutura robusta e estável em barril

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