Relevant bibliographies by topics / Nucleus Pulposus Regeneration

Academic literature on the topic 'Nucleus Pulposus Regeneration'

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Published: 6 September 2023

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Journal articles on the topic "Nucleus Pulposus Regeneration"

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Kregar Velikonja, Nevenka, Jill Urban, Mirjam Fröhlich, Cornelia Neidlinger-Wilke, Dimitris Kletsas, Urska Potocar, Sarah Turner, and Sally Roberts. "Cell sources for nucleus pulposus regeneration." European Spine Journal 23, S3 (December 3, 2013): 364–74. http://dx.doi.org/10.1007/s00586-013-3106-9.

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Yu, Lei, Zi-Jie Sun, Quan-Chang Tan, Shuang Wang, Wei-Heng Wang, Xiang-Qun Yang, and Xiao-Jian Ye. "Thermosensitive injectable decellularized nucleus pulposus hydrogel as an ideal biomaterial for nucleus pulposus regeneration." Journal of Biomaterials Applications 35, no. 2 (April 26, 2020): 182–92. http://dx.doi.org/10.1177/0885328220921328.

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Extracellular matrix loss is one of the early manifestations of intervertebral disc degeneration. Stem cell-based tissue engineering creates an appropriate microenvironment for long term cell survival, promising for NP regeneration. We created a decellularized nucleus pulposus hydrogel (DNPH) from fresh bovine nucleus pulposus. Decellularization removed NP cells effectively, while highly preserving their structures and major biochemical components, such as glycosaminoglycan and collagen II. DNPH could be gelled as a uniform grid structure in situ at 37°C for 30 min. Adding adipose marrow-derived mesenchymal stem cells into the hydrogel for three-dimensional culture resulted in good bioactivity and biocompatibility in vitro. Meanwhile, NP-related gene expression significantly increased without the addition of exogenous biological factors. In summary, the thermosensitive and injectable hydrogel, which has low toxicity and inducible differentiation, could serve as a bio-scaffold, bio-carrier, and three-dimensional culture system. Therefore, DNPH has an outstanding potential for intervertebral disc regeneration.
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Patt, Stephan, Mario Brock, Heinz Michael Mayer, Carl Schreiner, and Lamartine Pedretti. "Nucleus Pulposus Regeneration After Chemonucleolysis with chymopapain?" Spine 18, no. 2 (February 1993): 227–31. http://dx.doi.org/10.1097/00007632-199302000-00009.

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Risbud, Makarand V., Irving M. Shapiro, Alexander R. Vaccaro, and Todd J. Albert. "Stem cell regeneration of the nucleus pulposus." Spine Journal 4, no. 6 (November 2004): S348—S353. http://dx.doi.org/10.1016/j.spinee.2004.07.031.

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Krouwels, Anita, Juvita D. Iljas, Angela H. M. Kragten, Wouter J. A. Dhert, F. Cumhur Öner, Marianna A. Tryfonidou, and Laura B. Creemers. "Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration." International Journal of Molecular Sciences 21, no. 8 (April 14, 2020): 2720. http://dx.doi.org/10.3390/ijms21082720.

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Matrix production by nucleus pulposus (NP) cells, the cells residing in the center of the intervertebral disc, can be stimulated by growth factors. Bone morphogenetic proteins (BMPs) hold great promise. Although BMP2 and BMP7 have been used most frequently, other BMPs have also shown potential for NP regeneration. Heterodimers may be more potent than single homodimers, but it is not known whether combinations of homodimers would perform equally well. In this study, we compared BMP2, BMP4, BMP6, and BMP7, their combinations and heterodimers, for regeneration by human NP cells. The BMPs investigated induced variable matrix deposition by NP cells. BMP4 was the most potent, both in the final neotissue glysosaminoglycan content and incorporation efficiency. Heterodimers BMP2/6H and BMP2/7H were more potent than their respective homodimer combinations, but not the BMP4/7H heterodimer. The current results indicate that BMP4 might have a high potential for regeneration of the intervertebral disc. Moreover, the added value of BMP heterodimers over their respective homodimer BMP combinations depends on the BMP combination applied.
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Bodnarchuk, J. A., M. V. Khyzhnjak, О. О. Potapov, and N. G. Chopik. "BIOCHEMICAL AND BIOMECHANICAL SUBSTANTIATION OF REPARATIVE REGENERATION OF INTERVERTEBRAL DISCS IN PATIENTS WITH DEGENERATIVE DISC DISEASES." Eastern Ukrainian Medical Journal 8, no. 3 (2020): 249–54. http://dx.doi.org/10.21272/eumj.2020;8(3):249-254.

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Degenerative disc diseases occupy the second place in the overall structure of morbidity with temporary disability. In 40% of patients with spinal osteochondrosis, diseases of the locomotor apparatus and connective tissue cause primary disability. Disc degeneration is a pathological process that is the main cause of low back pain and is observed in the vast majority of people at some point in their lives. The influence of mechanical stress leads to degenerative changes in the tissues of the nucleus pulposus of the intervertebral disc. Limited transport and low cellular saturation of the discs hinder recovery, make the intervertebral disc particularly vulnerable to injury, and contribute to the appearance of morphological tissue damage associated with the processes of biological aging. The pathological process involves all structural elements of the intervertebral disc. The earliest manifestations of disc degeneration usually occur in the nucleus pulposus, where a reduced content of proteoglycans disrupts mechanical function, which leads to progressive morphological degeneration of the entire intervertebral segment. Existing treatment methods (both surgical and conservative) are not able to adjust the number of cells in the nucleus pulposus and are unable to stop the pathological process in the intervertebral disc. Prevention of degeneration or repair of the intervertebral disc is a potential treatment for lumbar pain syndromes. Cell therapy has become a subject of great interest, as new research reports significant regenerative potential for many cellular sources, including the regeneration of the nucleus pulposus region of the intervertebral disc. The use and implementation of modern cell therapy in practical neurosurgery allows us to approach the problem of intervertebral disc degeneration at a new qualitative level with the use of multipotent cells, biochemical peptides in the reparative processes of the nucleus pulposus, as a possibility of treatment and prevention of vertebrogenic pain syndromes in the future. Keywords intervertebral disc, nucleus pulposus, cell therapy, transplantation, degenerative changes, reparation
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Peng, Xuan, Lingjia Yu, Lin Shi, Huajun Dong, Xiaohui Meng, and Bin Zhu. "Polymeric hydrogels and decellularized nucleus pulposus extracellular matrix technology for nucleus pulposus repair and regeneration." Polymer Testing 117 (January 2023): 107854. http://dx.doi.org/10.1016/j.polymertesting.2022.107854.

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Zhu, Yanxia, Jie Tan, Hongxia Zhu, Guangyao Lin, Fei Yin, Liang Wang, Kedong Song, Yiwei Wang, Guangqian Zhou, and Weihong Yi. "Development of kartogenin-conjugated chitosan–hyaluronic acid hydrogel for nucleus pulposus regeneration." Biomaterials Science 5, no. 4 (2017): 784–91. http://dx.doi.org/10.1039/c7bm00001d.

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Hodgkinson, Tom, Francis Wignall, Judith A. Hoyland, and Stephen M. Richardson. "High BMPR2 expression leads to enhanced SMAD1/5/8 signalling and GDF6 responsiveness in human adipose-derived stem cells: implications for stem cell therapies for intervertebral disc degeneration." Journal of Tissue Engineering 11 (January 2020): 204173142091933. http://dx.doi.org/10.1177/2041731420919334.

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Stem cell–based regenerative strategies are promising for intervertebral disc degeneration. Stimulation of bone-marrow- and adipose-derived multipotent stem cells with recombinant human growth differentiation factor 6 (rhGDF6) promotes anabolic nucleus pulposus like phenotypes. In comparison to mesenchymal stem cells, adipose-derived multipotent stem cells exhibit greater NP-marker gene expression and proteoglycan-rich matrix production. To understand these response differences, we investigated bone morphogenetic protein receptor profiles in donor-matched human mesenchymal stem cells and adipose-derived multipotent stem cells, determined differences in rhGDF6 signalling and their importance in NP-like differentiation between cell populations. Bone morphogenetic protein receptor expression in mesenchymal stem cells and adipose-derived multipotent stem cells revealed elevated and less variable expression of BMPR2 in adipose-derived multipotent stem cells, which corresponded with increased downstream pathway activation (SMAD1/5/8, ERK1/2). Inhibitor studies demonstrated SMAD1/5/8 signalling was required for rhGDF6-induced nucleus-pulposus-like adipose-derived multipotent stem cell differentiation, while ERK1/2 contributed significantly to critical nucleus pulposus gene expression, aggrecan and type II collagen production. These data inform cell regenerative therapeutic choices for intervertebral disc degeneration regeneration and identify further potential optimisation targets.
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Li, Zhen, Keren Mevorat Kaplan, Abraham Wertzel, Marianna Peroglio, Boaz Amit, Mauro Alini, Sibylle Grad, and Avner Yayon. "Biomimetic fibrin–hyaluronan hydrogels for nucleus pulposus regeneration." Regenerative Medicine 9, no. 3 (May 2014): 309–26. http://dx.doi.org/10.2217/rme.14.5.

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Dissertations / Theses on the topic "Nucleus Pulposus Regeneration"

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Kim, Anne Jungjoo. "In situ regeneration of the nucleus pulposus." Diss., Search in ProQuest Dissertations & Theses. UC Only, 2009. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3359553.

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Thesis (Ph.D.)--University of California, San Francisco with the University of California, Berkeley, 2009.
Source: Dissertation Abstracts International, Volume: 70-06, Section: B, page: 3639. Adviser: Jeffrey C. Lotz.
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Simson, Jacob A. "Physical analysis of collagen-GAG composite scaffolds for nucleus pulposus tissue regeneration." Thesis, Massachusetts Institute of Technology, 2008. http://hdl.handle.net/1721.1/57874.

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Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering, 2008.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 27-28).
In this study biomaterial scaffolds for regeneration of nucleus pulposus were developed by freeze drying slurries with different proportions of collagen II (CII), chondroitin-6-sulfate (CS), and hyaluronic acid (HA). The scaffolds were analyzed using biochemical assays to determine final composition. Chemically cross-linked scaffolds were analyzed to determine pore size and cross-link density. It was determined that every material type contained large enough pore size (275 gm) to seed nucleus pulposus cells and mesenchymal stem cells. The addition of CS to the scaffold increased pore size. It was also found that increasing levels of CS and HA resulted in lower cross-link density. These materials will be used next in In Vitro studies to determine their viability as regenerative tissue engineering constructs.
by Jacob A. Simson.
S.B.
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Aladin, Kaderbatcha Darwesh Mohideen. "Nanoscale structure-property and macroscale biomechanical function of nucleus pulposus in health, disease and regeneration." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2010. http://hub.hku.hk/bib/B45197143.

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Ehlicke, Franziska [Verfasser]. "Entwicklung eines injizierbaren Zell-Matrix-Komposites zur Regeneration der Bandscheibe (Nucleus pulposus) : Einfluss verschiedener Stimuli auf die Differenzierung von humanen mesenchymalen Stammzellen in Richtung Nucleus pulposus-Zellen / Franziska Ehlicke." Aachen : Shaker, 2014. http://d-nb.info/1049379608/34.

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Strassburg, Sandra. "An In Vitro Culture System to Study Human Mesenchymal Stem Cell / Nucleus Pulposus Cell Interactions : Implications for Intervertebral Disc Regeneration." Thesis, University of Manchester, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521587.

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Rodrigues, Pinto Ricardo Pedro Ferreira. "Isolation and phenotypic characterisation of human notochordal cells : implications for the development of cell-based therapies for intervertebral disc degeneration." Thesis, University of Manchester, 2015. https://www.research.manchester.ac.uk/portal/en/theses/isolation-and-phenotypic-characterisation-of-human-notochordal-cells-implications-for-the-development-of-cellbased-therapies-for-intervertebral-disc-degeneration(8d5cbfdd-edd0-458c-a048-554f6a2c830b).html.

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Back pain is a highly prevalent condition whose pathogenesis is associated with intervertebral disc (IVD) degeneration. Degeneration is driven by abnormal cell biology, particularly within the IVD’s inner core, the nucleus pulposus (NP). In recent years, there has been an ever-increasing search for cell-based therapies aimed at correcting the cell biology and thus repairing/regenerating the degenerate IVD. The success of these novel therapies, however, requires a thorough understanding of IVD development and of the phenotype of its cells. The embryonic, foetal and juvenile NP is populated by large vacuolated notochordal cells that with skeletal maturity are replaced by smaller NP cells. Since notochordal cells have been shown to display protective and anabolic roles in the IVD their loss in humans has often been suggested to initiate the degenerative process. As such, a detailed understanding of notochordal cells and their regulatory pathways may help identify factors involved in IVD homeostasis and aid the development of novel cell-based therapies targeting IVD degeneration. The study of human notochordal cells has, however, been hindered by ethical, logistical and technical difficulties in obtaining suitable samples and, as such, the human notochordal cell phenotype is, to date, unknown, constituting a major limitation in the field. The work presented here was conducted with the objective of developing a methodology to isolate human developing notochordal cells (NP progenitors) from adjacent sclerotomal cells (annulus fibrosus and vertebral body progenitors), to characterise the notochordal cell phenotype and identify potential factors involved in notochordal cell biology. Initially, human embryonic and foetal spines were characterised to assess their suitability as a source of notochordal cells and to identify a notochord-specific marker that could be used to isolate notochordal cells for microarray studies. The human developing spine contained large vacuolated notochordal cells in all stages analysed (3.5-18 weeks post-conception (WPC)) that specifically expressed KRT8, KRT18 and KRT19 at all stages and CD24 between 5.5-18 WPC. KRT18 and CD24 were independently used to label notochordal cells (7.5-14 weeks post-conception) and separate them from sclerotomal cells. Methodologies were developed to allow extraction of RNA of sufficient quality for microarray analysis from fixed, permeabilised (in the case of KRT18) and/or, labelled and sorted cells (CD24). Microarray analysis identified and real-time qPCR and, for some markers, immunohistochemistry, validated GRB14, SLC19A1, FGF10, ADORA3, TBXA2R, CDH6, ANPEP, CD69, CD24, RTN1, PRPH, MAP1B, ISL1 and CLDN1 as human notochordal cell markers. Ingenuity pathway analysis was performed to investigate the pathways/networks and upstream regulators and downstream effectors of notochordal cells. Inhibition of inflammation and angiogenesis were identified as relevant to notochordal cell biology, function and, possibly, to the known protective and anabolic role notochordal cells display in the IVD. Notochordal marker gene expression was identified in adult NP tissue, and negatively correlated with degeneration. Proteins encoded by ADORA3 and MAP1B were expressed by a proportion of adult NP cells, suggesting the presence of notochord-derived cells in the adult NP.Importantly, this is the first study to detail a methodology and successfully isolate human notochordal cells. Such methodology has the potential to be used to culture and investigate the biology of viable human notochordal cells (CD24+ve). Future studies aimed at developing cell-based therapies for IVD degeneration could also use these identified markers to assess appropriate stem cell differentiation to notochordal cells.
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Khan, Shahnaz. "The effect of the intervertebral disc microenvironment on disc cell and mesenchymal stem cell behaviour : implications for disc degeneration and regeneration." Thesis, University of Manchester, 2013. https://www.research.manchester.ac.uk/portal/en/theses/the-effect-of-the-intervertebral-disc-microenvironment-on-disc-cell-and-mesenchymal-stem-cell-behaviour-implications-for-disc-degeneration-and-regeneration(b5629a75-4cb0-45d8-affb-2b936d9408e1).html.

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Intervertebral disc (IVD) degeneration is associated with low back pain (LBP). It has been suggested that changes in the IVD physio-chemical microenvironment (i.e. hypoxia, reduced nutrient and acidic conditions) may lead to disc degeneration. Studying the response of human nucleus pulposus (NP) cells to these conditions could establish the causal relationship between IVD microenvironment and aberrant cellular behaviour, characteristic of disc degeneration. Human bone marrow mesenchymal stem cells (BM-MSCs) are a promising cell population for disc regeneration. However, knowledge of their survival and functioning in the microenvironment of the IVD is still lacking. Moreover, in vitro co-culture model studies that are used to study MSC/disc cell interaction, also need to consider the effect of the microenvironment on cellular responses. BM-MSCs and degenerate NP cells were cultured alone or co-cultured in monolayer under hypoxia (2%O2), reduced nutritional (2% serum or/and 5mM glucose) and acidic (moderate pH 6.8 or severe pH 6.5) conditions alone or in combination for 7 days. Cell viability, proliferation, gene and protein expression was assessed. Degenerate NP cells and BM-MSCs maintained good cell viability under all conditions. Both cell types demonstrated overall similar proliferation and gene and protein responses under the majority of the conditions and combinations studied. Hypoxia promoted aggrecan and versican matrix biosynthesis in both cell types. Nutrient deprived and moderate acidic conditions (pH 6.8) inhibited proliferation of both cell types. Interestingly the combination of hypoxia with these conditions showed a protective effect in modulating cell proliferation. These results imply that hypoxia may be beneficial in some instances. Nutrient deprived conditions had a relatively minor effect on degenerate NP cell gene and protein expression but these conditions specifically inhibited VCAN expression in BM-MSCs. The combination of hypoxia with these conditions increased or restored VCAN expression. Interestingly the combination of hypoxia with reduced glucose conditions increased aggrecan and versican matrix biosynthesis in both NP cells and BM-MSCs. The combination of hypoxia and complete nutrient deprived conditions (both reduced serum and reduced glucose) impaired ACAN, VCAN and PAX-1 gene and aggrecan and versican protein expression in degenerate NP cells implicating disc hypoxia and complete nutrient deprived combined microenvironment in accelerating degenerate changes in NP cells. In contrast, these conditions showed no such detrimental effects on BM-MSC gene and protein expression. pH 6.5 was critical for both cell types proliferation and ACAN and VCAN gene expression suggesting that severe acidic conditions may exacerbate degenerative changes and be inhibitory for implanted MSCs. Finally, a combination of hypoxia, complete nutrient deprived and moderate acidic conditions, reduced cell proliferation without affecting the gene expression profile of both cell types. IVD-like physio-chemical microenvironmental conditions also appeared to influence differentiation of BM-MSC and modulation of degenerate NP cell phenotype observed during co-culture. Noticeably hypoxia, reduced serum or reduced glucose conditions stimulated BM-MSC differentiation and modulation of degenerate NP cell phenotype. Hypoxia also increased or recovered changes at gene expression level in both BM-MSCs and degenerate NP cells under nutrient deprived (reduced serum or/and reduced glucose) conditions during co-culture. Degenerate NP cell and BM-MSC co-culture also showed noticeable increase in aggrecan and versican biosynthesis under hypoxia and reduced glucose combine conditions, implicating these in improving the co-culture responses. Severe pH condition alone, pH 6.8 in combination with hypoxia and finally all IVD-like physio-chemical conditions together compromised co-culture responses. Such results imply that IVD-like physio-chemical microenvironmental conditions may influence MSC based regenerative outcomes. This work has increased our understanding about the influence of disc harsh microenvironment on degeneration and regeneration processes.
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Lok, Peter Yin Cheung. "Development of a novel minimally invasive scaffold system for spinal disc repair." Thesis, The University of Sydney, 2014. http://hdl.handle.net/2123/12583.

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Debilitating chronic back pain caused by severe spinal disc degeneration leads to loss of mobility, affecting quality of life, a significant loss of productivity for the employee and the employer. Currently available surgical intervention options, such as spinal fusion and total disc replacement, seeking only to alleviate pain, are not only invasive, but fail to address the underlying biological causes of spinal disc degeneration, or restore normal physiological spinal motion. Recently proposed tissue engineering approaches focus on stopping and reversing the degenerative cascade, which has a promising regenerative effect, though not without significant challenges before a clinical application is made available, including tumourigenesis risks and proof of efficacy. A minimally invasive nucleus pulposus replacement option, which preserves the competent annulus fibrosis, while replacing the removed degenerated nucleus tissue with a prosthesis, provides an alternative for early disc degeneration, though most commercially available types are at clinical trial stages. There is an opportunity for developing a minimally invasive nucleus pulposus replacement type spinal implant system that restores disc biomechanics and addresses biological degenerative causes. This body of work details the design, development, fabrication, prototyping, verification and validation of this novel implant system. The implant system consisted of a configuration of scaffold and hydrogel interpenetrating polymer network composite delivered minimally invasively via a cannula system, after the nucleus pulposus is removed in a nucleotomy with a set of specialised tools. Implantation of the novel prosthesis was shown to be successful in various spinal disc models, in meeting identified design and functional requirements, including biomechanical loading, resistance to expulsion and radiopacity.
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Francisco, Aubrey Therese. "Laminin-Functionalized Polyethylene Glycol Hydrogels for Nucleus Pulposus Regeneration." Diss., 2013. http://hdl.handle.net/10161/8204.

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Intervertebral disc (IVD) disorders and age-related degeneration are believed to contribute to low back pain. There is significant interest in cell-based strategies for regenerating the nucleus pulposus (NP) region of the disc; however, few scaffolds have been evaluated for their ability to promote or maintain an immature NP cell phenotype. Additionally, while cell delivery to the pathological IVD has significant therapeutic potential for enhancing NP regeneration, the development of injectable biomaterials that retain delivered cells, promote cell survival, and maintain or promote an NP cell phenotype in vivo remains a significant challenge. Previous studies have demonstrated NP cell - laminin interactions in the NP region of the IVD that promote cell attachment and biosynthesis. These findings suggest that incorporating laminin ligands into biomaterial scaffolds for NP tissue engineering or cell delivery to the disc may be beneficial for promoting NP cell survival and phenotype. In this dissertation, laminin-111 (LM111) functionalized poly(ethylene glycol) (PEG) hydrogels were developed and evaluated as biomaterial scaffolds for cell-based NP regeneration.

Here, PEG-LM111 conjugates with functional acrylate groups for crosslinking were synthesized and characterized to allow for protein coupling to both photocrosslinkable and injectable PEG-based biomaterial scaffolds. PEG-LM111 conjugates synthesized using low ratios of PEG to LM111 were found support NP cell attachment and signaling in a manner similar to unmodified LM111. A single PEG-LM111 conjugate was conjugated to photocrosslinkable PEG-LM111 hydrogels, and studies were performed to evaluate the effects of hydrogel formulation on immature NP cell phenotype in vitro. When primary immature porcine NP cells were seeded onto PEG-LM111 hydrogels of varying stiffnesses, softer LM111 presenting hydrogels were found to promote cell clustering and increased levels of sGAG production as compared to stiffer LM111 presenting and PEG-only gels. When cells were encapsulated in 3D gels, hydrogel formulation was found to influence NP cell metabolism and expression of proposed NP phenotypic markers, with higher expression of N-cadherin and cytokeratin 8 observed for cells cultured in softer (<1 kPa) PEG-LM111 hydrogels.

A novel, injectable PEG-LM111 hydrogel was developed as a biomaterial carrier for cell delivery to the IVD. PEG-LM111 conjugates were crosslinked via a Michael-type addition reaction upon the addition of PEG-octoacrylate and PEG-dithiol. Injectable PEG-LM111 hydrogel gelation time, mechanical properties, and ability to retain delivered cells in the IVD space were evaluated. Gelation occurred in approximately 20 minutes without an initiator, with dynamic shear moduli in the range of 0.9 - 1.4 kPa. Primary NP cell retention in cultured IVD explants was significantly higher over 14 days when cells were delivered within a PEG-LM111 hydrogel carrier, as compared to cells in liquid suspension.

The studies presented in this dissertation demonstrate that soft, LM111 functionalized hydrogels may promote or maintain the expression of specific markers and cell-cell interactions characteristic of an immature NP cell phenotype. Furthermore, these findings suggest that this novel, injectable laminin-functionalized biomaterial may be an easy to use and biocompatible carrier for delivering cells to the IVD.


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Tai, Pei-Wen, and 戴珮雯. "Developing hyaluronic acid-silk fibroin double-network hydrogel for nucleus pulposus regeneration." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/k52xdj.

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Books on the topic "Nucleus Pulposus Regeneration"

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Shine, Kristy Melissa. Tissue engineering of the intervertebral disc: A scaffold-based approach to nucleus pulposus regeneration. 2009.

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Book chapters on the topic "Nucleus Pulposus Regeneration"

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Grad, Sibylle, Mauro Alini, Daisuke Sakai, and Joji Mochida. "Cell Therapy for Nucleus Pulposus Regeneration." In Cells and Biomaterials for Intervertebral Disc Regeneration, 1–42. Cham: Springer International Publishing, 2010. http://dx.doi.org/10.1007/978-3-031-02580-8_1.

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Mercuri, Jeremy J., and Dan T. Simionescu. "Advances in Tissue Engineering Approaches to Treatment of Intervertebral Disc Degeneration: Cells and Polymeric Scaffolds for Nucleus Pulposus Regeneration." In Polymers in Nanomedicine, 201–31. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/12_2011_149.

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Conference papers on the topic "Nucleus Pulposus Regeneration"

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Cleary, Heather, Thomas Barkley, Adam Goodman, Michael Payne, John Virtue, Jennifer Vernengo, and Jennifer Kadlowec. "Design of a Bioreactor for Mechanical Stimulation of Adipose Derived Stem Cells for Intervertebral Disc Tissue Engineering." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80745.

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Lower back pain is one of the most common medical problems in the world [1], affecting between 70% and 85% of the US population at some point during their lives [2]. Disc degeneration is caused by biological changes in the disc, which result in dehydration of the nucleus pulposus (NP). The long term goal of this project is to treat disc degeneration with a tissue engineering strategy for the regeneration of the nucleus pulposus using messechymal stem cells derived from adipose tissue. It has been established in cartilage regeneration studies that cyclic compressive loading of stem cells is beneficial for tissue formation compared to static culture [3–7]. In this work, a bioreactor is being developed that can subject cell-seeded polymeric tissue engineering scaffolds to dynamic compressive forces. Ultimately, the bioreactor will be used to study the effects of different loading parameters on the production of new nucleus pulposus tissue from adipose-derived stem cells.
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Francisco, Aubrey T., Robert J. Mancino, Claire G. Jeong, Isaac O. Karikari, Robby D. Bowles, Stephen L. Craig, and Lori A. Setton. "Injectable and Photocrosslinkable Laminin Functionalized Biomaterials for Intervertebral Disc Regeneration." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80660.

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Biological and anatomical changes of intervertebral disc (IVD) degeneration frequently occur in the nucleus pulposus (NP) [1]. Changes in NP matrix composition coincide with the loss of a distinct notochord derived cell population [2],[3], which may have the potential to generate or maintain a functional NP-like matrix. Immature NP cells reside in an environment rich in laminin and express specific laminin-binding receptors [4],[5]. Additionally, NP cells attach in higher numbers to laminins as compared to cells isolated from other regions of the IVD [6]. Our initial work demonstrated that matrix protein and stiffness modulate NP cell-cell interactions upon surfaces [7], with results that suggest soft, laminin-functionalized hydrogels may be useful for promoting an NP-like cell phenotype.
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Tasci, Arzu, Ladina Ettinger, Stephen Ferguson, and Philippe Büchler. "The Role of Agarose Mechanical Response on the Matrix Synthesis of Nucleus Pulposus Cells: A Pilot Study." In ASME 2009 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2009. http://dx.doi.org/10.1115/sbc2009-206763.

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Low back pain is the most common spinal disorder and its main cause is intervertebral disc (IVD) degeneration. IVD has a major role of withstanding loads generated in the spine during daily activities. However, it has a limited capacity for self-repair. Since it has an avascular structure, the pathways it uses for regeneration is quite complex and not yet well understood. The mechanical stimulation studies on the cell seeded constructs revealed that cells regulate their biosynthetic activity with cyclic loading [1,2]. The mechanical properties of the scaffold might play an important role in the transmission of mechanical signals to the embedded cells. The objective of this study is to investigate the effect of agarose concentration on the amount of extracellular matrix synthesis in IVD cell seeded constructs under static culture and cyclic loading conditions.
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Houseman, C., M. Scro, S. Belverud, D. Chen, P. Razzano, M. Levine, D. A. Grande, and N. O. Chahine. "Effect of TGF-β3 on the Gene Expression of Intervertebral Disc Cells in 3D Pellet Cultures." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19246.

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Intervertebral disc (IVD) degeneration typically involves changes in the multiple constitutive tissues of the IVD. Many tissue repair efforts have focused on the use of differentiated disc cells or stem cells for the regeneration of an IVD in vitro. Consequently, successful long term culture of human disc cells is pivotal for tissue regeneration of the IVD. The aim of this study is to establish a long-term in vitro culture system for the growth of disc cells that maintain their phenotype based on the anatomical origin (annulus fibrosus (AF), nucleus pulposus (NP), or the vertebral end-plates (EP)). This maintenance of phenotype is crucial for examination of treatment efficacy, which is typically designed to induce regeneration of a single tissue type (i.e. injection of growth factors into the NP or anti-inflammatory treatment of the EPs).
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5

Orlansky, Amy S., John I. Boxberger, and Dawn M. Elliott. "The Dynamic Viscoelastic Properties of the Rat Lumbar Disc Are Decreased Following Nucleus Pulposus GAG Reduction." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192634.

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The intervertebral disc has the important biomechanical function of dissipating energy during spinal loading. With degeneration, the disc experiences, among other changes, a loss of mechanical function and degradation of its composition. Using a rat model of early disc degeneration by injection of Chondroitinase ABC (ChABC), glycosaminoglycan (GAG) content in the nucleus pulposus (NP) was reduced which altered neutral zone mechanical properties. The contribution of decreased NP GAG content to the dynamic viscoelastic properties has yet to be determined. The advantage of dynamic viscoelastic testing is that it provides both viscous and elastic stiffness values as a function of loading frequency. These methods have been employed previously in a rabbit disc regeneration model, in ligament under three modes of loading, and in NP under oscillatory shear and compression. Therefore, the objective of this study was to determine the viscoelastic behavior of a rat lumbar disc at several equilibrium strains and to quantify the impact of GAG reduction on this behavior. Our hypotheses were: 1) elastic stiffness would be greater, and viscous stiffness and loss angle would be lower with increased frequency, and 2) both elastic and viscous stiffness would be lower in the reduced GAG discs at all frequencies.
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6

Natarajan, Raghu N., Alejandro Espinoza, and Gunnar B. J. Andersson. "Effect of Needle Puncture Injury on Human Intervertebral Disc Mechanics." In ASME 2010 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2010. http://dx.doi.org/10.1115/sbc2010-19116.

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Diagnosis, repair and regeneration of the disc often necessitate needle injection to the nucleus pulposus through the annulus. Discography in which a radio opaque material is injected into the nucleus and electrothermal treatment involving inserting a catheter into the disc requires disruption of the annulus through needle puncture. Annulus puncture may also be required during placement of nucleus implants. Needle puncture is also used to inject growth factors, gene and cell therapy for regeneration of the disc. In animal models, disc degeneration is induced over time by needle puncture of the annulus. The severity of the degeneration depends on the magnitude of the annulus needle puncture. One thing that is not clear is how much of the observed changes in the disc biomechanics and biochemical changes are due to nucleus treatment and how much is due to annular disruption through needle puncture. Animal model studies have shown that significant changes in disc mechanics were noticed within 1 week of needle puncture with a large-gauge needle. Another in-vitro animal study showed that biomechanical changes were observed in the disc when the ratio of needle diameter to disc height is greater than 40%. All these studies were focused on the effect of small number of needle diameters and addressed using animal cadaver models. How these needle puncture injury studies on small and large animal models can be extrapolated to human conditions is still not known. Thus there is need to evaluate effect of range of needle puncture diameters in human lumbar disc biomechanics. The purpose of this study is, with the help of a finite element models, quantify the biomechanical effect due to varying size of needle punctures in a human lumbar intervertebral disc.
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Korecki, Casey L., Catherine K. Kuo, Rocky S. Tuan, and James C. Iatridis. "Effect of Age and Frequency on Intervertebral Disc Cell Response to Dynamic Compression." In ASME 2007 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2007. http://dx.doi.org/10.1115/sbc2007-176595.

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The intervertebral disc (IVD) is a unique orthopaedic tissue consisting of at least two cell types: fibroblast-like annulus fibrosus (AF) cells and chondrocyte-like nucleus pulposus (NP) cells. Culture of cells in 3D gel matrices (such as alginate or agarose), maintains the normal morphology and ECM molecule production of chondrocytes for extended periods of time and also allows the application of various forms of mechanical stimulation, such as hydrostatic or compressive loading. In vivo studies have shown IVD cells to be responsive to frequency, duration, and amplitude of mechanical load [1]. IVD literature on mechanobiology uses varying methodologies to apply dynamic loads (compression, hydrostatic forces), with different times of mechanical stimulation, differences in model systems (in vivo, tissue culture, cell culture), species, and ages, and an optimal loading protocol to stimulate extracellular matrix protein accumulation is unknown. The overall goal of this work is to evaluate the potential, and perhaps even feasibility, of mechanical stimulation for extracellular matrix (ECM) regeneration using intervertebral disc cells. Also of interest is whether cells from mature tissue are capable of serving as a potential cell source for future IVD regeneration [2,3].
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