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1

Myers, Meagan B., and Cynthia A. Schandl, eds. Clinical Applications of Nucleic Acid Amplification. New York, NY: Springer US, 2023. http://dx.doi.org/10.1007/978-1-0716-2950-5.

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2

A, Toranzos Gary, ed. Environmental applications of nucleic acid amplification techniques. Lancaster, PA: Technomic Pub. Co., 1997.

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3

Lee, Helen H., Stephen A. Morse, and Ørjan Olsvik, eds. Nucleic Acid Amplification Technologies Application to Disease Diagnosis. Boston, MA: Birkhäuser Boston, 1996. http://dx.doi.org/10.1007/978-1-4612-2454-9.

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4

Zhang, Shusheng, Sai Bi, and Xinyue Song, eds. Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Singapore: Springer Singapore, 2019. http://dx.doi.org/10.1007/978-981-13-7044-1.

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5

Lauerman, Lloyd Herman. Nucleic acid amplification assays for diagnosis of animal diseases. [Madison, Wis.]: American Association of Veterinary Laboratory Diagnosticians, 1998.

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6

Giulio, Pisani, ed. Nucleic acid amplification technology (NAT) for the detection of Hepatitis C Virus (HCV) in plasma pools: Validation report. Roma: Istituto superiore di sanità, 2000.

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7

New York Academy of Sciences. Pharmaceutical science to improve the human condition: Prix Galien 2010 : winners and finalist candidates of the Prix Galien USA, International, and Pro Bono Humanitarian Awards 2010. Malden, MA: Wiley Periodicals, 2011.

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8

McNerney, Ruth. Evaluation of the role of nucleic acid amplification tests in the routine diagnosis of tuberculosis in developing countries with a high prevalence of HIV infection: Report of a three year collaborative research project (1996-1999). Lusaka: s.n., 2000.

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9

Scialpi, Angela, and Alessio Mengoni, eds. La PCR e le sue varianti. Florence: Firenze University Press, 2008. http://dx.doi.org/10.36253/978-88-6453-159-5.

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The book "La PCR e le sue varianti" is designed as a reference tool for those whose laboratory activities deal with methods based on nucleic acid amplification. The text provides the theoretical bases of the polymerase chain reaction (PCR) and its variants (e.g. RT-PCR, quantitative PCR, isothermic PCR) in a rapid and concise manner and describes the principal applications used for genetic identification and the study of genetic polymorphism, in the form of a protocol that can be easily consulted by the users.
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10

Bernd, Kochanowski, and Reischl Udo, eds. Quantitative PCR protocols. Totowa, N.J: Humana Press, 1999.

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11

Saluz, H. P. A laboratory guide for in vivo studies of DNA methylation and protein/DNA interactions. Basel: Birkhäuser Verlag, 1990.

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12

Myers, Meagan, and Cynthia Schandl. Clinical Applications of Nucleic Acid Amplification. Springer, 2023.

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13

Environmental Applications of Nucleic Acid Amplification Technology. CRC, 1997.

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14

H, Lee Helen, Morse Stephen A, and Olsvik Ørjan, eds. Nucleic acid amplification technologies: Application to disease diagnosis. Cambridge, MA: Birkhäuser Boston, 1997.

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15

(Editor), Olsvik, Morse (Editor), and Lee (Editor), eds. Nucleic Acid Amplification Technologies: Application to Disease Diagnosis. Birkhäuser Boston, 1997.

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16

Helen H. Lee Stephen A. Morse. Nucleic Acid Amplification Technologies: Application to Disease Diagnosis. Birkhäuser Boston, 2011.

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17

Zhang, Shusheng, Sai Bi, and Xinyue Song. Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer, 2019.

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18

Zhang, Shusheng, Sai Bi, and Xinyue Song. Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine. Springer, 2019.

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19

O'Leary, Timothy J. Nucleic Acid Amplification Assays for Molecular Hematophathology: Approved Guidline. Natl Committee for, 2003.

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20

Lee, Helen, and Stephen A. Morse. Nucleic Acid Amplification Technologies: Application to Disease Diagnosis (Urban Tapestry Series). Eaton Publishing Company/Biotechniques Books, 1997.

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21

Kellems, Rodney E. Gene Amplification in Mammalian Cells: A Comprehensive Guide. Taylor & Francis Group, 2020.

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22

Kellems, Rodney E. Gene Amplification in Mammalian Cells: A Comprehensive Guide. Taylor & Francis Group, 2020.

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23

Caspari, Gregor. Nucleic Acid Amplification Techniques for Virus Detection in Transfusion Medicine (Infusionstherapie Und Transfusionsmedizi, 1-2). S Karger Pub, 1998.

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24

(Editor), Elena Hilario, and John MacKay (Editor), eds. Protocols for Nucleic Acid Analysis by Nonradioactive Probes (Methods in Molecular Biology). 2nd ed. Humana Press, 2007.

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25

Kao, Mou-Chieh. Development of DNA-tagged liposome biosensing devices and enzyme-linked dot blot assay in combination with nucleic acid sequence-based amplification for rapid detection of viable shiga toxin-producing escherichia coli. Ithaca, NY : Cornell University, 2000, 2003.

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26

White, Thomas J., Michael A. Innis, David H. Gelfand, and John J. Sninsky. PCR Protocols: A Guide to Methods and Applications. Elsevier Science & Technology Books, 2012.

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27

PCR Protocols: A Guide to Methods and Applications. Academic Press, 1989.

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28

PCR protocols: A guide to methods and applications. San Diego: Academic Press, 1990.

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29

PCR Protocols: A Guide to Methods and Applications. Academic Press, 1989.

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30

PCR Protocols: A Guide to Methods and Applications. Academic Press, 1989.

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31

(Editor), Michael A. Innis, David H. Gelfand (Editor), John J. Sninsky (Editor), and Thomas J. White (Editor), eds. PCR Protocols: A Guide to Methods and Applications. Academic Press, 1989.

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32

Kulkarni, Kunal, James Harrison, Mohamed Baguneid, and Bernard Prendergast, eds. Genitourinary medicine. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780198729426.003.0009.

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In the UK, the continued rise in sexually transmitted infections remains a key public health concern. Since the advent of HIV infection, many genitourinary medicine specialists have also undertaken the management of HIV and AIDS, and there has been a move towards closer links or integration with contraception/family planning under the umbrella of sexual health, creating a continually shifting and developing field. Advances in diagnostic technology, such as the recent nucleic acid amplification tests for gonorrhoea, continue to make this specialty as fascinating and satisfying as ever, combining the science of medicine with the art of clinical practice. This chapter covers some of these recent advances, focusing on the key clinical evidence currently shaping this specialty.
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33

Putman, Shannon B. Cervicitis and Vulvovaginitis. Oxford University Press, 2016. http://dx.doi.org/10.1093/med/9780199976805.003.0038.

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Cervicitis is inflammation and irritation of uterine cervix often caused by sexually transmitted infection (STI); it can lead to pelvic inflammatory disease, endometritis, and pregnancy complications. Vulvovaginitis (inflammation of vagina and/or vulva with itching, erythema, and mucopurulent discharge) may or may not be related to STI. Sexually active women 25 years or younger with STIs should be offered empiric treatment for gonorrhea and chlamydia in addition to counseling regarding safe sex and testing for syphilis, HIV, and viral hepatitis at the time of presentation and prior to nucleic acid amplification testing results. Sexual partners of women testing positive for gonorrhea, chlamydia, or trichomoniasis should be informed and treated to prevent reinfection, with patients and their partners abstaining from intercourse until treatment is complete and symptoms resolve. High-risk patients with STI should be retested at 6 months given recurrent infection rates. Prophylaxis, vaccination, high degree of suspicion, and early intervention can help improve morbidity and mortality.
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