Dissertations / Theses on the topic 'NSO cells'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'NSO cells.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
Sage, Elizabeth Ann. "The functional competence of animal cells in culture : the NSO cell proteome." Thesis, University of Kent, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.399618.
Full textDuncan, Philippa Jane. "The effect of hyperosmotic conditions on growth and recombinant protein production by NSO myeloma cells in culture." Thesis, Liverpool John Moores University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.403285.
Full textOsman, Jason John. "Response of GS-NSO mouse myeloma cells to pH fluctuations relevant to those found in large scale fermentation." Thesis, University of Reading, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393530.
Full textWaskow, Claudia, Bonin Malte von, Martin Wermke, Cosgun Kadriye Nehir, Christian Thiede, Martin Bornhauser, and Gerard Wagemaker. "In Vivo Expansion of Co-Transplanted T Cells Impacts on Tumor Re-Initiating Activity of Human Acute Myeloid Leukemia in NSG Mice." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-191633.
Full textYu, Yi-Hsin Biotechnology & Biomolecular Sciences Faculty of Science UNSW. "Role of the RNAi pathway in influenza a virus infected mammalian cells." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2008. http://handle.unsw.edu.au/1959.4/41545.
Full textWaskow, Claudia, Bonin Malte von, Martin Wermke, Cosgun Kadriye Nehir, Christian Thiede, Martin Bornhauser, and Gerard Wagemaker. "In Vivo Expansion of Co-Transplanted T Cells Impacts on Tumor Re-Initiating Activity of Human Acute Myeloid Leukemia in NSG Mice." Public Library of Science, 2013. https://tud.qucosa.de/id/qucosa%3A28097.
Full textWu, Mon-Han. "The effect of osmotic pressure on GS-NSO antibody production cell line." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439532.
Full textChen, Baiyu, and 陳白羽. "Suprachiasmatic nucleus projecting retinal ganglion cells in golden hamsters development, morphology and relationship with NOS expressingamacrine cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37238218.
Full textLong, Xiangtian. "Synthesis and Evaluation of Stimulatory Properties of Glycolipids for Natural Killer T Cells." BYU ScholarsArchive, 2009. https://scholarsarchive.byu.edu/etd/2133.
Full textChen, Baiyu. "Suprachiasmatic nucleus projecting retinal ganglion cells in golden hamsters development, morphology and relationship with NOS expressing amacrine cells." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B37238218.
Full textPiedade, Nuno André Reis. "Transcriptional and redox changes in Huntington´s disease human stem cells." Master's thesis, Universidade de Aveiro, 2017. http://hdl.handle.net/10773/23437.
Full textA doença de Huntington (HD, “Huntington’s disease”) é uma doença neurodegenerativa autossómica dominante, causada pela repetição de sequências CAG no gene HTT. As manifestações clínicas da doença incluem alterações motoras, cognitivas e psiquiátricas, e atualmente não existe cura para a HD. A utilização de células estaminais pluripotentes induzidas (iPSC, ”inducedpluripotent stem cells”) e células estaminais neurais (NSC, “neural stem cells”) fornecem um modelo adequado para o estudo dos eventos iniciais conducentes à neurodegenerescência na HD. Assim, este trabalho teve como objetivo analisar as alterações de transcrição que envolvem a biogénese mitocondrial e a resposta ao stresse oxidativo em células HD-iPSC e HD-NSC, comparativamente a células controlo.Foi avaliada a expressão de PGC-1α (“peroxisome proliferator-activated receptor gamma coactivator 1-alpha”), do fator de transcrição mitocondrial A (TFAM) e subunidades dos complexos da cadeia respiratória mitocondrial e de enzimas que regulam a atividade da piruvato desidrogenase (PDH) em HD-iPSC e HD-NSC. Os resultados mostraram uma diminuição dos níveis de mRNA de PGC-1α, TFAM e subunidades do complexo III (CYC1, MT-CYB e UQCR10), e um aumento de mRNA da subunidade ND1 do complexo I e da cinase1 da PDH (PDK1) em HDiPSCNas HD-NSC apenas se observou uma diminuição nos níveis de mRNA de PGC-1α. Em células HD-iPSC e HD–NSC detetaram-se também níveis aumentados de peróxido de hidrogénio, uma espécie reativa de oxigénio (ROS, “reactive oxygen species”), sem contudo ocorrerem alterações na acetilação no resíduo de lisina 68 da superóxido dismutase 2 (SOD2) nas HD-iPSC; curiosamente, observou-se uma diminuição significativa dos níveis de mRNA de UCP2 (”uncoupling protein 2”) em HD-iPSC e HD-NSC. De forma a avaliar a resposta antioxidante, analisámos ainda os níveis proteicos e de mRNA do Nrf2 (”nuclear factor erythroid 2–related factor 2”) e da subunidade catalítica da enzima gama-glutamilcisteína ligase (GCLc) e heme oxigenase 1 (HO-1). Não foram encontradas alterações nos níveis de mRNA ou proteicos de Nrf2, apesar de se ter verificado uma tendência para um aumento da sua fosforilação no resíduo de serina 40 (P(Ser40)-Nrf2) em células HD-iPSC, sugerindo uma resposta ao aumento de ROS. Verificou-se ainda um aumento da expressão de GCLc nas HD-iPSC. Os resultados demonstram que a alteração transcricional de proteínas envolvidas na biogénese mitocondrial e de subunidades de complexos mitocondriais, assim como elevados níveis de ROS, a que se associa um aumento de GCLc e uma redução de UCP2, constituem potenciais eventos iniciais envolvidos na patogénese da HD.
Huntington´s disease (HD) is an autosomal dominant neurodegenerative disorder caused by an abnormal expansion of CAG repeats in the HTT gene. Clinical manifestations of the disease include motor, cognitive and psychiatric changes, and currently there is no cure for HD. The use of induced-pluripotent stem cell (iPSC) and neural stem cell (NSC) provide reliable models to study early events involved in HD neurodegeneration.Thus, in this study we aimed to determine changes in transcripts related with mitochondrial biogenesis and the response to oxidative events in HD-iPSC and HD-NSC, when compared with the respective control cells. We investigated the expression of the transcription factor peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and its downstream targets, namely TFAM (mitochondrial transcriptional factor A) and subunits of mitochondrial complexes, and enzymes that regulate the activity of pyruvate dehydrogenase (PDH) in both HD-iPSC and HD-NSC. Our analysis revealed reduced mRNA levels of PGC-1α, TFAM and mitochondrial and nuclear-encoded complex III subunits (CYC1, MT-CYB and UQCR10) and enhanced mRNA levels of ND1 subunit of complex I and PDH kinase 1 (PDK1) in HD-iPSC. Interestingly, apart from PGC-1α, unchanged mRNA levels of other targets were observed in HD-NSC. In both HD-iPSC and HD-NSC we observed increased levels of hydrogen peroxide, a reactive oxygen species (ROS), but unchanged acetylation at Lys68 of superoxide dismutase 2 (SOD2 or Mn-SOD); nevertheless, we observed a significant reduction in the expression of uncoupling protein 2 (UCP2) in HD-iPSC and HD-NSC. To evaluate the antioxidant response, we further measured protein and mRNA levels of nuclear factor erythroid 2–related factor 2 (Nrf2) and two of its downstream targets, namely γ- glutamylcysteine ligase catalytic subunit (GCLc) and heme oxygenase 1 (HO-1). We did not find changes in mRNA expression nor protein levels of Nrf2, but we observed a tendency for increased Nrf2 phosphorylation at Ser40 (p-Nrf2) in HDiPSC, suggesting a slight response to ROS. Concomitantly, we found an increase in GCLc expression in HD-iPSC. Our results evidence reduced transcriptional changes associated with decreased mitochondrial biogenesis and complexes subunits, as well as enhanced ROS levels linked to increased GCLc and reduced UCP2 as potential early events in HD pathogenesis.
Ribeiro, Rodrigo Alves. "Avalia??o in vitro da ades?o e prolifera??o de c?lulas mesenquimais do ligamento periodontal humano em diferentes superf?cies de tit?nio." Universidade Federal do Rio Grande do Norte, 2011. http://repositorio.ufrn.br:8080/jspui/handle/123456789/17065.
Full textIn the last years, many scientific researches in implantology have been focused on alternatives that would provide higher speed and quality in the process of osseointegration. Different treatment methods can be used to modify the topographic and chemical properties of titanium surface in order to optimize the tissue-implant reactions by a positive tissue response. This study aimed to evaluate the adhesion and proliferation of mesenchymal cells from human periodontal ligament on two different titanium surfaces, using cell culture techniques. Grade II titanium discs received different surface treatments, forming two distinct groups: polished and cathodic cage plasma nitriding. Human periodontal ligament mesenchymal cells were cultured on titanium discs in 24-well cell culture plates, at a density of 2 x 104 cells per well, including wells with no discs as positive control. Data obtained by counting the cells that adhered to the titanium surfaces (polished group and cathodic cage group) and to the plastic surface (control group), in the 24, 48 and 72-hour periods after plating, were used to analyze cell adhesion and proliferation and to obtain the cell growing curve in the different groups. The data were submitted to nonparametric analysis and the differences between groups were compared by Kruskal-Wallis and Friedman statistical tests. No statistically significant differences were found in the cells counts between the groups (p>0.05). It was concluded that both treatments produced surfaces compatible with the adhesion and proliferation of human periodontal ligament mesenchymal cells
Nos ?ltimos anos, v?rias pesquisas cient?ficas em Implantodontia t?m buscado alternativas que proporcionem maior rapidez e qualidade no processo de osseointegra??o. Diferentes m?todos de tratamento podem ser utilizados para modificar as propriedades topogr?ficas e qu?micas da sua superf?cie do tit?nio, a fim de otimizar as rea??es tecido-implante atrav?s de uma resposta tecidual favor?vel. O presente trabalho teve como objetivo analisar a ades?o e prolifera??o de c?lulas mesenquimais de ligamento periodontal humano a diferentes superf?cies de tit?nio, atrav?s de t?cnicas de cultivo celular. Discos de tit?nio grau II receberam diferentes tratamentos de superf?cie, constituindo dois grupos distintos: polido e nitretado a plasma na configura??o de gaiola cat?dica. C?lulas mesenquimais obtidas do ligamento periodontal de dentes humanos h?gidos foram cultivadas sobre os discos de tit?nio em placas de cultivo de 24 po?os, na densidade de 2 x 104 c?lulas/po?o, incluindo po?os sem discos como controle positivo. Os dados obtidos das contagens das c?lulas aderidas ?s superf?cies de tit?nio (grupo polido e grupo gaiola cat?dica) e ? superf?cie pl?stica (grupo controle), nos intervalos de 24, 48 e 72 horas ap?s o plaqueamento, foram utilizados para analisar a ades?o e prolifera??o celular e obter a curva de crescimento celular nos diferentes grupos. Os dados foram submetidos a an?lises n?o param?tricas e as diferen?as entre os grupos foram comparadas pelos testes estat?sticos Kruskal-Wallis e Friedman. N?o foram encontradas diferen?as estat?sticas significativas nas contagens celulares entre os grupos estudados (p>0,05). Conclui-se que ambos os tratamentos produziram superf?cies compat?veis com ades?o e prolifera??o de c?lulas mesenquimais do ligamento periodontal humano
Schneider, Júlia. "Efeito do sulfato de dehidroepiandrosterona (SDHEA) sobre células foliculares nos estágios antral inicial e pré-ovulatório." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2017. http://hdl.handle.net/10183/157962.
Full textOvarian follicle is formed by the oocyte (female gamete) and somatic follicular cells. Those closer to the oocyte are cumulus oophorus cells (CCs), which are in direct contact with the female gamete, and the granulosa mural cells (GCs), which form the wall of the ovarian follicle. As GCs and CCs are easily accessed during assisted reproduction procedures and are discarded after oocyte retrieval, they can be used in research aimed at elucidating ovarian physiology. However, when recovered in assisted reproduction cycles, these cells are in a luteinized state due patient hormonal treatment. It is known that the use of luteinized GCs to study the molecular ovulatory process is limited due to this prior cellular exposure to gonadotrophins and their luteinized state. However, luteinized CGs have been shown to reacquire similar characteristics to those of non-luteinized follicles in early stages of differentiation (non-luteinized early antral). Stimulation of these cells with follicle stimulating hormone (FSH) increases expression of genes that characterize CGs typical of pre ovulatory follicles (non-luteinized pre ovulatory). Another important question regarding the ovarian follicle relates to androgens action in this ovarian structure. As it is known, androgen receptor activation, located in follicular cells, is able to modulate expression and activity of important genes for the maintenance of ovarian follicle development. Thus, authors suggest that the reproductive effect of dehydroepiandrosterone (DHEA) and their sulfate (SDHEA) treatment, important androgens, may be due their actions precisely in the follicular microenvironment. Consequently, the aim of this work was to analyze the exposure to SDHEA of non-luteinized follicular cells (early antral and pre-ovulatory stages). Granulosa and cumulus cells were obtained from patients submitted to in vitro fertilization and were separately cultivated. Initially, the best culture time of this proposed cellular model was determined among 6, 8 or 10 days of culture. Cellular viability analysis showed that primary follicular culture for the next steps of the study would be of 8 days. Thereafter, cellular viability assays were used to determine the best SDHEA dose among 5 doses to follicular cells exposure in comparison to a control without hormonal exposure. The analysis showed that the best dose to use was 0,08 μM of SDHEA. Subsequently, after defined the best culture time and the ideal exposure dose of the cells to SDHEA, experiments were performed with two different experimental groups: non-luteinized early antral cells and non-luteinized pre ovulatory cells – exposed to FSH. Both groups were divided in two subgroups: control group (no hormonal exposure) and SDHEA group (with SDHEA exposure). SDHEA, estradiol and progesterone hormonal dosages of the cell culture supernatant were done on days 1, 4, 6 and 8. Over time analysis revealed that SDHEA values were constant in control group during all the cell culture period, without statistical difference between the four hormonal dosages performed in this group. However, treated group showed a difference in the values of this hormone on days 6 and 8, compared to days 1 and 4, due to treatment with SDHEA of these experimental group . Regarding estradiol, independent of cell type and stage of development, it was possible to see that its secretion was high on the first day of culture, decreasing in others due to conditions and time of culture of the non-luteinized cells protocol. In addition, the SDHEA treated cells presented higher estradiol secretion than those not treated. Finally, progesterone dosages revealed that treatment with SDHEA did not alter this hormone secretion from the cells in either of the two development stages. Besides that, the cells had an increased progesterone secretion on the sixth cell culture day compared to first and fourth day; however, this secretion began to decrease on the eight day of culture. In conclusion, SDHEA treatment is able to increases the non-luteinized follicular cells secretion of estradiol, but it is not able to modify the progesterone secretion of the same cells. More studies are needed to better understand the effects of SDHEA on the process that make part of folliculogenesis.
Bond, Jennifer Emma. "Investigation of the dynamic relationship between extracellular and intracellular pH in GS-NSO cell culture." Thesis, Imperial College London, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.415045.
Full textMocherla, Supriya. "Inhibitory Effects of Growth Factors on Proliferation of Porcine Smooth Muscle Cells in the Direct Co-culture System." VCU Scholars Compass, 2007. http://scholarscompass.vcu.edu/etd_retro/142.
Full textKhoo, Soo Hean Gary. "Molecular profiling of the increased antibody productivity in a proliferation arrested NSO mouse myeloma cell line." Thesis, University of Birmingham, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.521990.
Full textChapman, Laurie A. "Interactions of nutrients on methyl mercury toxicity in neuron X spinal chord hybrid cells (NSC-34) and human oligodendrocyte X rhabdomyosarcoma cells (MO3.13)." Thesis, McGill University, 2001. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=36888.
Full textVasou, Andri. "Development of a novel cell-based screening platform to identify inhibitors of viral interferon antagonists from clinically important viruses." Thesis, University of St Andrews, 2016. http://hdl.handle.net/10023/8266.
Full textFerreira, Luciana Corrêa [UNESP]. "Efeitos do LPS bacteriano nos processos funcionais e de diferenciação de células progenitoras da polpa dentária." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/127660.
Full textOs processos de reparo da polpa dentária frente a procedimentos conservadores passam, em geral, pelo confronto entre possíveis microrganismos presentes na área da exposição e as células responsáveis pela diferenciação e produção de tecido mineralizado. Entretanto, a literatura ainda necessita de informações sobre o efeito direto de produtos bacterianos sobre este processo. O objetivo deste estudo foi avaliar os efeitos do lipopolissacarídeo (LPS) bacteriano (E. coli) sobre células progenitoras da polpa dental, utilizando-se condições basais e com mineralização préinduzida por meio osteogênico. Para isso, células progenitoras caracterizadas foram submetidas a diferentes concentrações de LPS bacteriano (com ou sem indutor da mineralização [lM]), para observação da atividade de fosfatase alcalina (ALP). A concentração mínima de 200 ng/ml, para se verificar alteração de atividade enzimática de ALP, foi usada para se observar os efeitos funcionais de mineralização (pelo alizarin vermelho - ARS), expressão das citocinas IL-1β e TNF-α, além da expressão dos genes DSPP e DMP-1. Os dados quantitativos foram analisados por ANOVA e teste de Tukey. As células em meio osteogênico com as diferentes concentrações de LPS apresentaram baixa atividade da ALP no curto prazo, comparadas às tratadas com meio α-MEM. Estas apresentaram alta atividade, comparadas ao controle. 200 ng/ml do LPS afetaram o processo de mineralização ao longo do tempo, reduzindo a ação de mineralização dos grupos que o associaram ao indutor de mineralização. Houve expressão de IL-1β e TNF-α para todos os grupos em todos os tempos, além disso, para a IL-1β, o grupo que associou os 200ng/ml de LPS com o meio indutor após 7 dias apresentou maior expressão. Houve expressão do gene DMP-1 e não expressão de DSPP e ocorreu uma maior expressão gênica nos grupos tratados com meio indutor da mineralização no gene DMP-1 . Esses achados
Dental pulp repair processes due to conservative procedures are, in general, affected by the conflicts between possible microorganisms at the area of exposure and cells responsible for differentiation and production of mineralized tissue. However, the literature still lacks information on how bacterial products affect these processes directly. The purpose of this study was to evaluate the bacterial lipopolysaccharide (E. coli LPS) effects on dental pulp progenitor cells, regarding the expression of differentiation genes and function of mineralization, using basal conditions and pre-induced mineralization by osteogenic media. Characterized progenitor cells was initially submitted to different LPS concentrations (with or without osteogenic medium [lM]), to observe alkaline phosphatase activity (ALP). The minimal concentration (200 ng/ml) to observe phosphatase enzymatic activity was used to assess the functional mineralization effects (by alizarin red staining - ARS), cytokine production (IL-1β e TNF-α), besides the gene expression for odontoblast differentiation markers (DSPP and DMP-1). Quantitative data will be statistically analyzed by ANOVA and Tukey's test. Cells in osteogenic medium with different concentrations of LPS showed low ALP activity shortterm compared to those treated with α-MEM. These showed high activity, compared to control. 200 ng/ml of LPS did affect the mineralization process over time, reducing the action of mineralization of the groups that have associated LPS with the IM. There was expression of IL-1β and TNF-α for all groups at all times, additionally, IL-1β for the group that has associated 200 ng/ml of LPS with osteogenic media after 7 days showed higher expression. There was expression of DMP-1 and DSPP genes and the expression in groups treated with IM was greater in DMP-1. These findings suggest that the inflammatory potential of LPS on the pulp progenitor cells contribute to an early mineralization ...
Ferreira, Luciana Corrêa. "Efeitos do LPS bacteriano nos processos funcionais e de diferenciação de células progenitoras da polpa dentária /." São José dos Campos, 2014. http://hdl.handle.net/11449/127660.
Full textBanca: Cláudio Antonio Talge Carvalho
Banca: Aletéia Massula de Melo Fernandes
Resumo: Os processos de reparo da polpa dentária frente a procedimentos conservadores passam, em geral, pelo confronto entre possíveis microrganismos presentes na área da exposição e as células responsáveis pela diferenciação e produção de tecido mineralizado. Entretanto, a literatura ainda necessita de informações sobre o efeito direto de produtos bacterianos sobre este processo. O objetivo deste estudo foi avaliar os efeitos do lipopolissacarídeo (LPS) bacteriano (E. coli) sobre células progenitoras da polpa dental, utilizando-se condições basais e com mineralização préinduzida por meio osteogênico. Para isso, células progenitoras caracterizadas foram submetidas a diferentes concentrações de LPS bacteriano (com ou sem indutor da mineralização [lM]), para observação da atividade de fosfatase alcalina (ALP). A concentração mínima de 200 ng/ml, para se verificar alteração de atividade enzimática de ALP, foi usada para se observar os efeitos funcionais de mineralização (pelo alizarin vermelho - ARS), expressão das citocinas IL-1β e TNF-α, além da expressão dos genes DSPP e DMP-1. Os dados quantitativos foram analisados por ANOVA e teste de Tukey. As células em meio osteogênico com as diferentes concentrações de LPS apresentaram baixa atividade da ALP no curto prazo, comparadas às tratadas com meio α-MEM. Estas apresentaram alta atividade, comparadas ao controle. 200 ng/ml do LPS afetaram o processo de mineralização ao longo do tempo, reduzindo a ação de mineralização dos grupos que o associaram ao indutor de mineralização. Houve expressão de IL-1β e TNF-α para todos os grupos em todos os tempos, além disso, para a IL-1β, o grupo que associou os 200ng/ml de LPS com o meio indutor após 7 dias apresentou maior expressão. Houve expressão do gene DMP-1 e não expressão de DSPP e ocorreu uma maior expressão gênica nos grupos tratados com meio indutor da mineralização no gene DMP-1 . Esses achados
Abstract: Dental pulp repair processes due to conservative procedures are, in general, affected by the conflicts between possible microorganisms at the area of exposure and cells responsible for differentiation and production of mineralized tissue. However, the literature still lacks information on how bacterial products affect these processes directly. The purpose of this study was to evaluate the bacterial lipopolysaccharide (E. coli LPS) effects on dental pulp progenitor cells, regarding the expression of differentiation genes and function of mineralization, using basal conditions and pre-induced mineralization by osteogenic media. Characterized progenitor cells was initially submitted to different LPS concentrations (with or without osteogenic medium [lM]), to observe alkaline phosphatase activity (ALP). The minimal concentration (200 ng/ml) to observe phosphatase enzymatic activity was used to assess the functional mineralization effects (by alizarin red staining - ARS), cytokine production (IL-1β e TNF-α), besides the gene expression for odontoblast differentiation markers (DSPP and DMP-1). Quantitative data will be statistically analyzed by ANOVA and Tukey's test. Cells in osteogenic medium with different concentrations of LPS showed low ALP activity shortterm compared to those treated with α-MEM. These showed high activity, compared to control. 200 ng/ml of LPS did affect the mineralization process over time, reducing the action of mineralization of the groups that have associated LPS with the IM. There was expression of IL-1β and TNF-α for all groups at all times, additionally, IL-1β for the group that has associated 200 ng/ml of LPS with osteogenic media after 7 days showed higher expression. There was expression of DMP-1 and DSPP genes and the expression in groups treated with IM was greater in DMP-1. These findings suggest that the inflammatory potential of LPS on the pulp progenitor cells contribute to an early mineralization ...
Mestre
Startzman, Ashley N. "Inhibition of stat3 protein as an approach to sensitizing ovarian cancer cells to cisplatin." Honors in the Major Thesis, University of Central Florida, 2008. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1143.
Full textBachelors
Medicine
Molecular Biology and Microbiology
Matusica, Dusan, and matu0012@flinders edu au. "Regulation of p75NTR Trafficking by Neurotrophins in the NSC-34 Motor Neuron Cell Line." Flinders University. School Of Medicine, 2008. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20080808.115027.
Full textWatatani, Yosaku. "Molecular heterogeneity in peripheral T-cell lymphoma, not otherwise specified revealed by comprehensive genetic profiling." Kyoto University, 2020. http://hdl.handle.net/2433/253204.
Full textGianfrancesco, Anthony Giacomo. "Multi-wavelength characterization of cadmium telluride solar cell: Development of Q-EBIC and NSOM measurement techniques." Digital WPI, 2013. https://digitalcommons.wpi.edu/etd-theses/213.
Full textShyu, Duan-Liang. "Rescue of host innate immunity in pigs infected with Nsp1ß mutant PRRSV." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1437049612.
Full textZhang, Lei. "Exploring Electron Transfer Dynamics of Novel Dye Sensitized Photocathodes : Towards Solar Cells and Solar Fuels." Doctoral thesis, Uppsala universitet, Fysikalisk kemi, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-302263.
Full textOscarsson, Johan. "Towards Mixed Molecular Layers for Dye-Sensitized Solar Cells : A Photoelectron Spectroscopy Study." Doctoral thesis, Uppsala universitet, Molekyl- och kondenserade materiens fysik, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-301164.
Full textPhilip, Diana Liz. "The Influence of Synthetic Microenvironments in Determining Stem Cell Fate." University of Akron / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1627669247178055.
Full textSpens, Erika. "Development of a protein-free fed-batch process for NS0 cells: studies on regulation of proliferation." Doctoral thesis, Stockholm : Department of Bioprocess Technology, School of Biotechnology, Royal Institute of Technology, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4162.
Full textRutherford, Claire. "The role of platelet derived growth factor-BB and basic fibroblast growth factor in neo-intimal and fatty streak formation in models of experimental atherosclerosis." Thesis, Queen Mary, University of London, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.313562.
Full textBorba, Claudio Carneiro. "Fatores que influenciaram nos resultados das coletas de células progenitoras hematopoéticas em crianças portadoras de neuroblastoma avançado." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5141/tde-08082016-083044/.
Full textObjectives: To evaluate the results of peripheral hematopoietic CD34+ stem cells harvesting in children with neuroblastoma treated at Serviço de Oncologia e Hematologia do Instituto da Criança do Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo; regarding age, weight, stimulation with chemotherapy, G-CSF dose, time between diagnosis and the mobilization beginning and therapeutic 131I-MIBG use and the influence in mobilization and peripheral harvesting of autologous hematopoietic stem cells and to associate the amount of CD34+ cells obtained with the patient\'s clinical evolution. Methods: Between January 1989 and June 2012, children with neuroblastoma underwent to mobilization and peripheral hematopoietic stem cell harvesting and were retrospectively analyzed. Results: The charts of 45 children were reviewed. Median age was 3.1 years (0-12years), and 26 (57%) had metastases in bone marrow at diagnosis. Average time between diagnosis and mobilization was 19.7 ± 12 months (median, 15.8 months). 11/45 (24.4%) received therapeutic 131I-MIBG prior to mobilization. The average G-CSF dose was 26.5 ± 5.3mg/kg/day (mean 28mg/kg/day). There was no correlation between the absolute number of peripheral CD34+ cells and age (p=0.9), weight (p=0.63), G-CSF dose (p=0.46) or the range between diagnosis and early mobilization (p=0.09). The median quantification of CD34+ cells/uL in peripheral blood was 36.6, average 45.2 ±42.6 (minimum 1.7 and maximum 236.3 CD34+ cells/uL). Patients who had received therapeutic 131I-MIBG prior to mobilization, showed fewer absolute amount of CD34+/uL cells in peripheral blood (p=0.04). In 26 patients (57.8%) it was possible to harvest more than 2.0 x106 CD34+ cells/kg at first apheresis and in 19 patients (42.2%) more than one collection were necessary, and eight patients (17.8 %) failure to mobilize. Patients presenting less than 12 CD34+ cells/uL in peripheral blood on the harvesting day failed to reach more then 2.0x106 cells CD34+/kg in 81.8% of the apheresis procedures. It was infused a median number of 2.66 x106 CD34+ cells/kg (mean 1.6 ± 3.38; min 1.8; max 8.74 x106 CD34+ cells/kg). After the stem cells infusion, patients had white blood cells count greater than 1000/mm3 and platelet greater than 50,000/mm3 for two consecutive days on average after 13 ±10 and 46 ± 33 days, respectively. Conclusions: The hematopoietic stem cells harvesting was feasible in all patients included in this report. The G-CSF dose, age, weight and the period between harvesting and diagnosis showed no influence in mobilization and harvesting of autologous hematopoietic stem cells, however the absolute number of peripheral blood CD34+ cells/uL is an important predictive factor for the harvesting outcome. Additionally our findings support for the first time the notion that the use of therapeutic 131I-MIBG could have a negative impact in mobilization of peripheral blood stem cells in children with neuroblastoma
Santos, Pedro Paulo de Andrade. "Express?o imuno-histoqu?mica da triptase em mast?citos nos fibromas de c?lulas gigantes e hiperplasias fibrosas de mucosa oral." Universidade Federal do Rio Grande do Norte, 2008. http://repositorio.ufrn.br:8080/jspui/handle/123456789/17103.
Full textConselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico
The giant cell fibroma is a benign neoplasm characterized by the presence of mono, bi or multinucleate cells, which can have a connection to the presence of mast cells. This research aims to analyze, descriptively and comparatively, the immunohystochemistry expression of the tryptase in mast cells of the giant cell f ibroma, f ibrous hyperplasia and samples of the normal oral mucosa. Thirty cases of giant cell fibroma, ten cases of fibrous hyperplasia and ten cases of normal oral mucosa were selected for the analysis of the immunohistochemistry expression, determination of the number of present mast cells, as well as their location and shape. It could be stated that there was a statistically signif icant difference (p<0,001) in relation to the quantity of mast cells among other samples analyzed where the giant cell f ibroma presented lesser quantity of mast cell and the hyperplasia showed higher concentration of this cellular type. Although the oral mucosa has presented a higher quantity of mast cells when compared to the giant cells fibroma, these were found in usual locations in the connective tissue in normal tissues. There could be noticed a statistically significant difference in relation to the number of non-granulated mast cells (p<0,001). On the areas of fibrosis, we could observe a statistically signif icant difference (p<0,006) among the samples. In relation to the present mast cells in perivascular location, no statistically signif icant difference was found. On the morphological analysis there was a predominance of oval mast cells. It was concluded that despite of the fact there was a lesser quantity of mast cells present in cases of giant cell f ibroma, they appeared to have a stronger relation to the present giant fibroblasts in this lesions, around 59,62%, being also evidenced a strong relation between these cells and the fibrosis areas in both cases of giant cell f ibroma and f ibrous hyperplasias and samples of normal oral mucosa, used as control group in our study, confirming, this way, the role of the mast cells as fibrinogenous inductor
O fibroma de c?lulas gigantes constitui-se de uma neoplasia benigna, caracterizada pela presen?a de c?lulas gigantes, mono, bi ou multinucleadas, c?lulas estas que podem guardar rela??o com a presen?a de mast?citos. O prop?sito desta pesquisa consistiu em analisar descritiva e comparativamente a express?o imuno-histoqu?mica da triptase em mast?citos de fibroma de c?lulas gigantes, hiperplasia fibrosa e esp?cimes de mucosa oral normal. Foram selecionados 30 casos de fibroma de c?lulas gigantes, 10 casos de hiperplasia fibrosa e 10 casos de mucosa oral normal, para a an?lise da express?o imuno-histoqu?mica, determina??o do n?mero de mast?citos presentes, bem como a sua forma e localiza??o. Constatou-se diferen?a estatisticamente significativa (p<0,001) em rela??o a quantidade de mast?citos entre os esp?cimes analisados, onde o fibroma de c?lulas gigantes apresentou a menor quantidade de mast?citos e a hiperplasia exibiu a maior concentra??o deste tipo celular. Embora a mucosa oral tenha apresentado uma maior quantidade de mast?citos quando comparado com os casos de f ibroma de c?lulas gigantes, estes se encontravam em localiza??es usuais no tecido conjuntivo em tecidos normais. Verif icou-se, diferen?a estatisticamente significativa, no que diz respeito ao n?mero de mast?citos n?o degranulados (p<0,001). Nas ?reas de fibrose, observamos diferen?a estatisticamente signif icativa (p<0,006) entre os esp?cimes. Com rela??o aos mast?citos presentes em localiza??o perivascular n?o se observou diferen?a estatisticamente significativa. Na an?lise morfol?gica verif icou-se uma predomin?ncia de mast?citos ovais. Concluiu-se que embora uma menor quantidade de mast?citos estivesse presente nos casos de fibroma de c?lulas gigantes, estes exibiam maior rela??o com os fibroblastos gigantes presentes nestas les?es em torno de 59,62%, sendo evidenciada tamb?m uma forte rela??o entre estas c?lulas e ?reas de fibrose tanto nos casos de fibroma de c?lulas gigantes como de hiperplasias fibrosas e esp?cimes de mucosa oral normal, utilizados como controle em nosso estudo, confirmando desta forma, o papel dos mast?citos como indutor fibrinog?nico
Freitas, Adriana Regina de Oliveira. "Caracterização das vias de sinalização desencadeadas pelas interações PrPc-p66 e PrPc-laminina e sua relevância nos processos de morte celular programada e consolidação da memória." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-20072018-181445/.
Full textPrPc is an extremely conserved 35 KDa glycoprotein which seems to be essential during the transmission and pathogenesis of several neurodegenerative diseases like bovine spongiform encephalopathy or Creutzfeldt-Jacob disease (PRUSINER, 1991). Although the physiological function of this protein remains unclear, it is well established that prion diseases require PrPc expression and are often characterized by deposition of an abnormal PrPc isoform, named PrPsc (GABIZON et. al., 1997). Interested in the normal fuction of PrPc, our group has been dedicated to study the interations that PrPc could entertain with other molecules. We have identified and characterized two interactions in which PrPc is involved: with a 66 KDa ligand protein, recently identified as the STI1 protein (ZANATA et. al., 2002) and with laminin (GRANER et. al., 2000). In this work, we have investigated the signaling pathways triggered by these interactions, as well as their relevance in programmed cell death and memory formation mechanisms. We show in this work that PrPc-p66 interaction transduces neuroprotective signals through a cAMP/PKA-dependent pathway in the neuroblastic layer of rodents\' retina. Moreover, we demonstrated that PrPc-laminin interaction has an important role for short-term memory formation through the activation of cAMP-PKA-MAPK pathways and for long-term memory with the activation of the cAMP/PKA pathway only.
Yoshito, Walter Kenji. "Estudo de rotas de síntese e processamento cerâmico do compósito NiO-YSZ para aplicação como anodo em células a combustível do tipo óxido sólido." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/85/85134/tde-01062011-154148/.
Full textThis study aim the definition of synthesis and ceramic processing conditions of the anodic component suitable for operation of SOFC, i.e, homogeneous distribution of NiO in YSZ matrix and porosity after reduction above 30%. The selected synthesis routes included the co-precipitation in ammonia media, mechanical mixing of powders and combustion reaction from nitrate salts. The characterization techniques of powders included the X-ray diffraction, scanning and transmission electron microscopy, laser diffraction, nitrogen gas adsorption technique (BET) and Helium pycnometry. The obtained results indicated that the loss of Ni2+ in co-precipitation process, due to the formation of complex [Ni(NH3)n]2+, can be minimized by controlling the pH around 9.3, keeping the concentration of nickel cation in the solution to be precipitated around 0.1M. In the mechanical mixing method the best condition of powder dispersion, without differential sedimentation, was obtained for zeta potential values at pH around 8.0, fixing the dispersant concentration at 0.8%. For the combustion synthesis it was observed that when stoichiometric and twofold stoichiometric urea was used, amorphous phase was formed and a higher surface area was attained in the final products. Employing the fuel-rich solution condition, crystallization of the powder was observed and the relative intensity of reflections of XRD patterns increased with excess of fuel, due to increasing the reaction temperature. Sinterability studies of pellets prepared from powder synthesized by the three routes described above showed the temperature around 1300 º C for maximum rate densification and porosity between 6.0 and 14%. Reduction results of the composites confirmed that the reduction kinetics occurs in two steps. The first one with a linear behavior and controlled by chemical reaction on the surface. The second reduction step is the reduction that is controlled by gas diffusion in micro pores, generated by reduction of nickel oxide, decreasing the rate of reduction.
Najima, Yuho. "Induction of WT1 specific human CD8+ T cells from human HSCs in HLA class I Tg NOD/SCID/Il2rgKO mice." Kyoto University, 2016. http://hdl.handle.net/2433/215440.
Full textKyoto University (京都大学)
0048
新制・課程博士
博士(医学)
甲第19614号
医博第4121号
新制||医||1015(附属図書館)
32650
京都大学大学院医学研究科医学専攻
(主査)教授 髙折 晃史, 教授 山田 亮, 教授 三森 経世
学位規則第4条第1項該当
Andrade, Mariana Macedo Costa de. "O efeito da tolerância à endotoxina nos linfócitos T regulatórios e Th 17." Universidade de São Paulo, 2016. http://www.teses.usp.br/teses/disponiveis/5/5164/tde-20092016-155050/.
Full textThe control of pathological immune responses (autoimmunity, allergy, transplant rejection) has been a major goal of immunologists. Despite recent advances, most current treatments still seeks to reduce immunity and inflammation rather than restore the healthy state of immune tolerance. Sepsis is a disease triggered by the presence of bacteria and / or bacterial products like lipopolysaccharide (LPS), the main component of the outer membrane of gram-negative bacteria, activating the immune response of the host. The characterization of lymphocyte profile in response to LPS tolerance is extremely important for the study of immunosuppression in sepsis contribution. The aim of this study was to investigate whether the proven reduction in mortality seen previously in animal sepsis model by tolerance to LPS, can be associated with the increase in population of CD4 + regulatory and Th17. Mice C57 / 6 mice received subcutaneous (s.c.) injection of LPS (1mg / kg) for 5 days, followed by cecal ligation and puncture (CLP). Cytokines and marked lymphocytes were measured during after tolerance and CLP challenge. Both subtypes of T cells Treg and Th17 analyzed showed an increase of these cells in the spleen during and after tolerance. This study demonstrated that reduced mortality after previously seen tolerance may be associated with increasing the population of regulatory T cells and Th17 because immunoregulation of the hiperinflamação and neutrophil recruitment
SKODA, SANDRO. "Hidrodinâmica do escoamento nos canais catódicos de um célula a combustível de membrana polimérica condutora de prótons." reponame:Repositório Institucional do IPEN, 2014. http://repositorio.ipen.br:8080/xmlui/handle/123456789/11813.
Full textMade available in DSpace on 2014-11-10T12:39:03Z (GMT). No. of bitstreams: 0
Tese (Doutorado em Tecnologia Nuclear)
IPEN/T
Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
Brown, Jennifer L. "A molecular and immunological investigation of cellular responses to dengue virus identification of potentially upregulated host genes and the constructionof a vaccinia virus expressing the dengue 1 Hawaii NS3 protein." Link to electronic version, 2000. http://www.wpi.edu/Pubs/ETD/Available/etd-0330100-124248/.
Full textBaptistella, Jamila Cristina [UNESP]. "Caracterização das células-tronco oriundas da geléia de Wharton do cordão umbilical de bovinos nos três trimestres de gestação (Bos indicus)." Universidade Estadual Paulista (UNESP), 2016. http://hdl.handle.net/11449/143804.
Full textApproved for entry into archive by Juliano Benedito Ferreira (julianoferreira@reitoria.unesp.br) on 2016-09-06T19:50:07Z (GMT) No. of bitstreams: 1 baptistella_jc_dr_araca.pdf: 2482721 bytes, checksum: 505b73636d8fa1498b06642b2343ed60 (MD5)
Made available in DSpace on 2016-09-06T19:50:07Z (GMT). No. of bitstreams: 1 baptistella_jc_dr_araca.pdf: 2482721 bytes, checksum: 505b73636d8fa1498b06642b2343ed60 (MD5) Previous issue date: 2016-08-01
A possibilidade para o isolamento de células mesenquimais multipotentes de anexo fetal bovino é uma perspectiva interessante devido potencial para utilização destas células em aplicações biotecnológicas. No entanto, ainda há pouco conhecimento disponível sobre as características destas células progenitoras na espécie bovina. Proliferando a morfologia das células, a partir de três fases da gestação, tipicamente apareceu forma de fuso do tipo fibroblastos, apresentando o mesmo número e viabilidade. Além disso, a capacidade proliferativa das células T em resposta a um estímulo mitogénico foi suprimida quando as células da geléia de Wharton foram incluídas na cultura. Multilinhagens foram confirmadas pela sua capacidade adipogênica, condrogénica e diferenciação neurogênica. O presente estudo demonstrou que células tronco mesenquimais colhidas a partir da geléia de Wharton do cordão umbilical de bovino em todas as fases da gestação mostraram capacidade proliferativa, potencial privilegiado e imunológico.
The possibility for isolating bovine mesenchymal multipotent stromal cells from fetal adnexa is interesting prospect because of the potential for these cells to be used for biotechnological applications. However, little knowledge is available about the characteristics of these progenitor cells in the bovine species. Proliferating cell morphology, from three stages of pregnancy, typically appeared fibroblast-like spindle shape, presenting the same viability and number. Moreover, the proliferative capacity of T cells in response to a mitogen stimulus was suppressed when Wharton’s jelly mesenchymal stem cells were included in the culture. Multilineage properties were confirmed by their ability to undergo adipogenic, chondrogenic and neurogenic differentiation. The study demonstrated that Wharton’s jelly mesenchymal stem cells harvested from bovine umbilical all pregnancy stages showed proliferative capacity, privileged and stem ness potential.
Baptistella, Jamila Cristina. "Caracterização das células-tronco oriundas da geléia de Wharton do cordão umbilical de bovinos nos três trimestres de gestação (Bos indicus) /." Araçatuba, 2016. http://hdl.handle.net/11449/143804.
Full textBanca:Marcos Antonio Maioli
Banca:Roberto Gameiro de Carvalho
Banca:Marcos Roberto Bonuti
Banca:Luiz Eduardo Correa Fonseca
Resumo: A possibilidade para o isolamento de células mesenquimais multipotentes de anexo fetal bovino é uma perspectiva interessante devido potencial para utilização destas células em aplicações biotecnológicas. No entanto, ainda há pouco conhecimento disponível sobre as características destas células progenitoras na espécie bovina. Proliferando a morfologia das células, a partir de três fases da gestação, tipicamente apareceu forma de fuso do tipo fibroblastos, apresentando o mesmo número e viabilidade. Além disso, a capacidade proliferativa das células T em resposta a um estímulo mitogénico foi suprimida quando as células da geléia de Wharton foram incluídas na cultura. Multilinhagens foram confirmadas pela sua capacidade adipogênica, condrogénica e diferenciação neurogênica. O presente estudo demonstrou que células tronco mesenquimais colhidas a partir da geléia de Wharton do cordão umbilical de bovino em todas as fases da gestação mostraram capacidade proliferativa, potencial privilegiado e imunológico.
Abstract: The possibility for isolating bovine mesenchymal multipotent stromal cells from fetal adnexa is interesting prospect because of the potential for these cells to be used for biotechnological applications. However, little knowledge is available about the characteristics of these progenitor cells in the bovine species. Proliferating cell morphology, from three stages of pregnancy, typically appeared fibroblast-like spindle shape, presenting the same viability and number. Moreover, the proliferative capacity of T cells in response to a mitogen stimulus was suppressed when Wharton's jelly mesenchymal stem cells were included in the culture. Multilineage properties were confirmed by their ability to undergo adipogenic, chondrogenic and neurogenic differentiation. The study demonstrated that Wharton's jelly mesenchymal stem cells harvested from bovine umbilical all pregnancy stages showed proliferative capacity, privileged and stem ness potential.
Doutor
Riehs, Daniel. "Make Your Data Work for You: True Stories of People and Technology." Thesis, Boston College, 2006. http://hdl.handle.net/2345/422.
Full textTechnology should enhance the human experience. Instead, it often alienates people from aspects of life that are considered most important. Artists are separated from their works, friends are separated from each other, and human ingenuity is filtered though computers before it can impact the world. These five short stories focus mainly on alienations inherent to communications and media technology, but also touch on database management and copyright concerns. Some take place in the present day; others present views of the future. All five stories use fiction to explore the truth of humanity's absurd relationship to technology
Thesis (BA) — Boston College, 2006
Submitted to: Boston College. College of Arts and Sciences
Discipline: College Honors Program
Nihei, Jorge Sadao. "Participação das células T de memória e linfócitos CD4.CD25+Foxp3 (reguladores) nos mecanismos de resistência à infecção experimental pelo trypanosoma cruzi." Universidade Federal da Bahia, 2013. https://www.arca.fiocruz.br/handle/icict/7129.
Full textMade available in DSpace on 2013-10-11T17:20:48Z (GMT). No. of bitstreams: 1 Jorge Sadao Nihei.pdf: 1524347 bytes, checksum: eb6417af423845038169df9f7824ead9 (MD5) Previous issue date: 2013
Universidade Federal da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
A infecção pelo Trypanosoma cruzi (T. cruzi) resulta em uma resposta imune que controla a predominância do parasito, mas é incapaz de eliminá-lo completamente, levando a persistência do parasito. A gravidade da doença de Chagas está correlacionada com a persistência do parasito nos tecidos musculares, neurais e intestinais. A função imunorregulatória das células T reguladoras em limitar a resposta imune poderia ser explorada no sentido de erificar a sua quantidade e qualidade no modelo experimental de infecção por T. cruzi. O principal objetivo deste projeto foi estudar a participação e influência dos linfócitos Treg sobre os mecanismos de resistência a infecção por T. cruzi, principalmente sobre a geração/manutenção de células T CD4+ e CD8+ memória efetora e central. Para tal, foi realizado um estudo comparativo da importância dos linfócitos T, e alterações das células Treg na infecção experimental por T. cruzi, de animais susceptíveis (C57Bl/6) e resistentes (BALB/c) nos dias 10, 20 e 30 apos a infecção. Adicionalmente, com o objetivo de investigar o papel das células T reguladoras, foi realizada a neutralização da citocina IL-2, com o tratamento in vivo com anti-IL-2 monoclonal (clone JES6-1A12), em animais C57Bl/6, durante a infecção com a cepa Tulahuen de T. cruzi. A análise da cinética de infecção (10, 20 e 30 dias) revelou que os animais resistentes (BALB/c) apresentaram maior expansão de células T efetoras CD4+ e CD8+ no baço e no tecido muscular esquelético. Os dados coletados demonstram que o tratamento com anti-IL-2 resultou em diminuição significativa da população de células T CD4+CD25+Foxp3+ nos animais infectados, comparado ao grupo infectado e tratado com imunoglobulina de rato. A diminuição das células Treg nestes animais resultou em alterações importantes nos mecanismos efetores como, por exemplo: o aumento da expansão das células T CD4+ e CD8+ efetoras e a produção de citocinas pró-inflamatórias e anti-inflamatórias no baço e tecido muscular esquelético. O reflexo destas alterações foi relacionado à diminuição da parasitemia e da mortalidade dos animais. Desta forma, as células Treg parecem exercer um controle da resposta imune efetora ao T. cruzi no sentido de tentar controlar a disseminação do patógeno e, ao mesmo tempo, impedir a destruição tecidual decorrente de uma exacerbação da resposta imune. Portanto, sugere-se que as células Treg estariam participando da resposta imune ao T. cruzi no sentido de modular a resposta imune efetora.
The Trypanosoma cruzi (T. cruzi) infection results in an immune response that controls the parasite prevalence, but is unable to eliminate it completely, leading to infection persistence. The severity of Chagas’ disease is correlated with the parasite persistence in the muscle, neural and bowel tissues. The immunorregulatory function of regulatory T cells in limiting the immune response could be exploited in order to verify its quantity and functional activities in an experimental model of T. cruzi infection. The main objective of this project was to study the participation and influence of Treg lymphocytes on the mechanisms of resistance to T. cruzi infection, mainly on generation and maintenance of central/effector memory T cells. In the first step, it was conducted a comparative study of the importance of T lymphocytes and alterations of Treg cells in experimental T. cruzi infection in susceptible (C57BL/6) and resistant (BALB/c) animals for 10, 20 and 30 days of infection. Additionally, in order to investigate the role of regulatory T cells, it was performed the neutralization of the cytokine IL-2, with the in vivo treatment with anti-IL-2 monoclonal antibody (clone JES6-1A12) of C57Bl/6 animals during infection with Tulahuen strain of T. cruzi. The analysis of the kinetics of infection (10, 20 and 30 days) revealed that the resistant animals (BALB/c) showed greater expansion and effector activity of the CD4+ and CD8+ splenic T cells and skeletal muscle tissue. The collected data demonstrate that the treatment with anti-IL-2 resulted in significant reduction of CD4+CD25+Foxp3+ T cells population in infected animals, compared to those infected and untreated. The decrease of Treg cells in those animals resulted in significant changes in the effector mechanisms such as: an increase in expansion of CD4+ and CD8+ T effector cells, and production of pro-inflammatory and anti-inflammatory cytokines in the spleen and skeletal muscle tissue. The reflection of these changes was verified by the decrease in parasitemia and mortality of the animals. Thus, Treg cells seem to have control of the effector immune response to T. cruzi in trying to control the spread of the pathogen and, at the same time, preventing tissue destruction due to an exacerbation of the immune response. Therefore, it is suggested that Treg cells would be participating in the immune response to T. cruzi by modulating the effector immune response.
Bureš, František. "Nové laboratorní úlohy v prostředí NS3." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2017. http://www.nusl.cz/ntk/nusl-361719.
Full textAlmeida, Monique Rocha. "Desenvolvimento de um dispositivo fotoeletroqu?mico a base de g-C3N4, Cu2O e CuO para clivagem da ?gua em H2 e O2." UFVJM, 2016. http://acervo.ufvjm.edu.br/jspui/handle/1/1311.
Full textApproved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-04-20T19:40:16Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) monique_rocha_almeida.pdf: 4635045 bytes, checksum: 2d203824a390ae82a5006e68b621c98b (MD5)
Made available in DSpace on 2017-04-20T19:40:16Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) monique_rocha_almeida.pdf: 4635045 bytes, checksum: 2d203824a390ae82a5006e68b621c98b (MD5) Previous issue date: 2016
Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM)
A convers?o de energia solar em energia qu?mica usando c?lulas fotoeletroqu?micas ? uma estrat?gia interessante para armazenar energia. C?lulas fotoeletroqu?micas s?o dispositivos constitu?dos de fotoeletrodos semicondutores que absorvem luz com energia maior ou igual a energia de bandgap do semicondutor e geram cargas reativas (el?trons e buracos) na superf?cie dos fotoeletrodos capazes de promover a redu??o e oxida??o da ?gua em H2 e O2, respectivamente. Nesta disserta??o, quatro fotoeletrodos de g-C3N4, g-C3N4/Cu1%, g- C3N4/Cu5% e Cu2O/CuO foram preparados com o objetivo de desenvolver uma c?lula fotoeletroqu?mica para clivagem da ?gua em H2 e O2 de forma espont?nea. As medidas de difratometria de raios X confirmaram a presen?a das fases g-C3N4 e Cu2O/CuO nos fotoeletrodos. As imagens de MEV mostraram que os materiais ? base de g-C3N4 possuem morfologia do tipo esponja, enquanto a heterojun??o Cu2O/CuO ? formada por nanopart?culas de forma indefinida. Medidas de reflect?ncia difusa mostraram que o acoplamento do g-C3N4 e Cu2O/CuO resulta em uma melhora significativa na absor??o ?ptica dos fotoeletrodos. Medidas de ?rea espec?fica indicaram que os nanomateriais ? base de g-C3N4 tem alta ?rea superficial (?100 m2 g?1), enquanto a ?rea espec?fica da heterojun??o Cu2O/CuO foi de 17 m2 g?1. Os resultados de redu??o ? temperatura programada evidenciaram a forma??o das heterojun??es. Os testes fotoeletroqu?micos de produ??o de O2 a partir da ?gua usando luz vis?vel indicaram que em potenciais an?dicos, apenas o fotoanodo de g-C3N4 foi est?vel apresentando uma densidade de fotocorrente de 16 ?A cm?2 que corresponde a uma efici?ncia de convers?o de luz de 0,014%. Em potenciais cat?dicos, a maior densidade de fotocorrente (60 ?A cm?2) foi obtida para o fotoeletrodo Cu2O/CuO. A efici?ncia de convers?o de luz do fotocatodo de Cu2O/CuO foi de 0,029%. Com base nos dados obtidos, uma c?lula fotoeletroqu?mica p-n foi constru?da usando a heterojun??o Cu2O/CuO como fotocatodo e g- C3N4 como fotoanodo. Esta c?lula gerou uma densidade de fotocorrente in operando de 0,62 ?A cm?2 e uma fotovoltagem de 0,62 V. A efici?ncia de convers?o solar da fotoc?lula foi de 0,004% sob irradia??o de luz vis?vel. Apesar da baixa efici?ncia obtida, espera-se que esta disserta??o possa servir de inspira??o para o desenvolvimento de novos dispositivos fotoeletroqu?micos para clivagem da ?gua em H2 e O2, usando luz vis?vel.
Disserta??o (Mestrado) ? Programa de P?s-Gradua??o em Qu?mica, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2016.
The conversion of solar energy into chemical energy using photoelectrochemical cells is an interesting strategy to store energy. Photoelectrochemical cells are made up of semiconductor photoelectrodes that absorb light with energy equal or higher than the bandgap energy of the semiconductor to generate reactive charges (electrons and holes) on the surface of the photoelectrodes, which can promote the oxidation and reduction reactions of water to form H2 and O2, respectively. In this dissertation, four photoelectrodes of g-C3N4, g-C3N4/Cu1%, g- C3N4/Cu5%, and Cu2O/CuO were prepared in order to develop a photoelectrochemical cell for spontaneous water splitting into H2 and O2. The X-ray diffraction patterns confirmed the presence of g-C3N4 and Cu2O/CuO phases in the photoelectrodes. The SEM images showed that the materials based on g-C3N4 have sponge-like morphology, whereas the Cu2O/CuO heterojunction is formed by nanoparticles with undefined shapes. Diffuse reflectance measurements showed that coupling g-C3N4 and Cu2O/CuO results in a significant improvement in optical absorption of the photoelectrodes. Surface area measurements indicated that the nanomaterials based on g-C3N4 have high surface areas (?100 m2 g?1), while the specific area for the Cu2O/CuO heterojunction was 17 m2 g?1. The temperature programmed reduction results evidenced the formation of the heterojunctions. The photoelectrochemical assays of O2 production from water using visible light indicated that at anodic potentials, only the photoanode g-C3N4 was stable showing a photocurrent density of 16 ?A cm?2, which corresponds to a light conversion efficiency of 0.014%. At cathodic potentials, the higher photocurrent density (60 ?A cm?2) was obtained for the Cu2O/CuO photoelectrode. The light conversion efficiency of the Cu2O/CuO photocathode was 0.029%. Based on the obtained data, a p-n photoelectrochemical cell was constructed using the Cu2O/CuO heterojunction as the photocathode and g-C3N4 as the photoanode. This photocell generated a photocurrent density in operando of 0.62 ?A cm?2 and photovoltage of 0.62 V. The light conversion efficiency of the photocell was 0.004% under visible light irradiation. Despite the low efficiency obtained for the p-n photocell, it is expected that this dissertation may serve of inspiration for the development of new photoelectrochemical devices for water splitting into H2 and O2 using visible light.
Schade-Weskott, Mathilde L. "Interaction of the African horsesickness virus NS3 protein with selected cellular proteins and its importance in viral egress from mammalian cells." Diss., University of Pretoria, 2014. http://hdl.handle.net/2263/65853.
Full textDissertation (MSc)--University of Pretoria, 2018.
Microbiology and Plant Pathology
MSc
Unrestricted
Melo, Edilailsa Janu?rio de. "Hematitas nanoparticuladas como fotocatalisadores potenciais para degrada??o qu?mica de contaminantes org?nicos em meio aquoso e a produ??o de hidrog?nio por fragmenta??o molecular da ?gua e da am?nia." UFVJM, 2017. http://acervo.ufvjm.edu.br/jspui/handle/1/1371.
Full textApproved for entry into archive by Rodrigo Martins Cruz (rodrigo.cruz@ufvjm.edu.br) on 2017-06-22T12:28:16Z (GMT) No. of bitstreams: 2 edilailsa_januario_melo.pdf: 2845467 bytes, checksum: 55f2a32335b696129bb30309f301b7ee (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)
Made available in DSpace on 2017-06-22T12:28:16Z (GMT). No. of bitstreams: 2 edilailsa_januario_melo.pdf: 2845467 bytes, checksum: 55f2a32335b696129bb30309f301b7ee (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017
Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES)
O desenvolvimento de novos semicondutores fotocatalisadores, em particular os ativos na produ??o de hidrog?nio por fragmenta??o molecular da ?gua e da am?nia (presente no lixiviado de biodigestores ou de aterros sanit?rios e em efluentes industriais), tem dominado a ordem priorit?ria de interesse nas tecnologias avan?adas para a gera??o de energia limpa. Este trabalho teve como objetivo principal avaliar a produ??o fotocatalisada de hidrog?nio gasoso, a partir da fragmenta??o molecular da ?gua ou da am?nia, por uso da hematita pura ou da hematita dopada com os c?tions met?licos cobalto, n?quel, cobre e zinco como materiais semicondutores. Os materiais preparados por coprecipita??o foram caracterizados pelas t?cnicas de EDX, MEV, DRX, espectroscopia M?ssbauer, FTIR, BET e XPS. A taxa da produ??o de hidrog?nio foi avaliada por medidas de densidade da corrente gerada pelo H2(g) evolu?do da fragmenta??o molecular da ?gua ou da am?nia, em c?lula fotoeletroqu?mica (PEC). Foram tamb?m realizados testes fotocatal?ticos, sob luz vis?vel, com as hematitas, pura e com dopantes, como fotocatalisadores para degrada??o do corante ?ndigo de carmim, utilizado como mol?cula modelo, simulando a decomposi??o de substrato org?nico poluente em ?gua presente em efluentes industriais. Os resultados dos testes de degrada??o do corante mostraram que as hematitas dopadas com cobre e zinco tiveram relativamente alta atividade na degrada??o do corante; os melhores resultados foram obtidos com a hematita com zinco. Na evolu??o do hidrog?nio da fotocat?lise da ?gua, a dopagem com c?tions met?licos n?o alterou significativamente a atividade fotoeletroqu?mica da hematita. Ainda assim, a amostra de hematita dopada com n?quel foi a que apresentou um discreto aumento da densidade de corrente, maior propor??o de hidrog?nio gasoso produzido, sob radia??o com comprimento de onda maior do que 450 nm. A densidade de corrente gerada da degrada??o da am?nia foi maior, se comparada ? fragmenta??o da ?gua. No entanto, a dopagem tamb?m n?o alterou de forma significativa a atividade PEC dos materiais. Das amostras de hematitas dopadas, a com cobre foi a que apresentou os melhores resultados fotoeletroqu?micos, ainda que abaixo da efici?ncia fotoeletroqu?mica da hematita pura.
Disserta??o (Mestrado) ? Programa de P?s-gradua??o em Biocombust?veis, Universidade Federal dos Vales do Jequitinhonha e Mucuri, 2017.
Technological developments of semiconductors to be used as photocatalysts for hydrogen production and for the degradation of organic pollutants in water in different circumstances, such as ammonia sluggishness from biodigesters and organic residues from industrial effluents, are strongly challenging the interest of the scientific community. The main objective of this work was to evaluate the molecular hydrogen production from the molecular fragmentation of water and ammonia using pure hematite and cobalt, nickel copper and zinc dopant prepared as co-precipitators as semiconductor materials. The materials prepared were characterized by EDX, MEV, XRD, M?ssbauer, FTIR, BET and XPS spectroscopy. The evaluation of the hydrogen production was carried out through measurements of current densities generated by the decomposition of water and ammonia in a photoelectrochemical cell (PEC). Photocatalytic tests were also carried out under visible light using pure hematite and with dopants as photocatalysts for the degradation of carmine indigo dye used as a model molecule. The results of the dye degradation tests showed that copper and zinc doped hematite increased dye degradation, and the best results were obtained for zinc hematite. In the evolution of hydrogen from water, the doping with metallic cations did not significantly alter photoelectrochemical activity of hematite, the hematite with nickel was the sample that presented a small increase in current density when in the presence of light. The current density generated by the ammonia degradation and consequent hydrogen production was higher when compared to water, however, doping also did not significantly alter the PEC activity of the materials, comparing the materials, the hematite with copper was the sample that presented the best results.
Barbosa, Tercio Augusto Penteado 1979. "Historicidade e atualidade do estudo da célula nos livros didáticos de ciências do ensino fundamental." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/253957.
Full textDissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Educação
Made available in DSpace on 2018-08-24T19:06:58Z (GMT). No. of bitstreams: 1 Barbosa_TercioAugustoPenteado_M.pdf: 4688716 bytes, checksum: 2bb450c8f464324b5c030e751371737d (MD5) Previous issue date: 2014
Resumo: Considerando a importância das células como unidades fundamentais da vida, estas se tornam uma questão central no ensino de ciências, sendo um dos conceitos mais complexos para o aluno de ensino fundamental ao se iniciar o estudo dos seres vivos. A dificuldade de abstração dos estudantes para compreendê-las e contextualizá-las se torna frequentemente um problema, pois estes estão acostumados ao que é concreto em seu cotidiano, mas as células, pelo seu tamanho, somente são vistas com auxílio de microscópios. Portanto, é necessária uma ruptura com as ordens de grandeza e o senso comum dos estudantes, sendo essencial que entendam a importância das células para os seres vivos também através de uma perspectiva histórica. Para que isso ocorra, os estudantes devem substituir sua visão da ciência como uma coleção de fatos e teorias estagnadas pela compreensão de que o conhecimento científico é passível de contínua correção temporal. Atualmente diversas pesquisas mencionam a importância e a relevância da história da ciência nas salas de aula, nos diversos níveis de ensino, podendo esta ser um instrumento importante ao professor que, utilizando-se de fontes adequadas e atualizadas, pode promover aos seus alunos uma visão mais crítica em relação à ciência e a construção do conhecimento científico. Constatamos, nesta investigação, que ainda é pequena a presença desses conteúdos nas escolas. Isto se deve, principalmente, à escassez de material disponível, tanto ao professor quanto às escolas. Buscando superar esse hiato, realizamos uma extensa pesquisa bibliográfica da história do descobrimento das células, suas estruturas e funções, estabelecendo uma linha do tempo com diversos episódios históricos que permitiram o avanço do conhecimento sobre as células, evitando um recorte histórico comparativo entre os episódios. Em seguida, fizemos uma análise do conteúdo das coleções didáticas de ciências melhor avaliadas pelo PNLD do período 2011/2013, objetivando descrever a estrutura e o padrão de distribuição do conteúdo sobre células, destacando suas características e propriedades. Além disso, buscamos descrever as unidades e/ou capítulos específicos dessas obras didáticas que tratem a história do descobrimento e desenvolvimento dos estudos sobre células, buscando identificar o tipo de abordagem histórica (internalista ou externalista) presente nessas unidades e/ou capítulos, quando houve. Em nossa avaliação dos livros didáticos verificamos que há uma grande deficiência de conteúdos contextualizados sobre as células e sua historicidade. Acreditamos que uma abordagem que considere uma perspectiva histórica, e que relacione descobertas importantes com tecnologias presentes em nosso cotidiano, poderia contribuir de forma significativa com a compreensão dos conceitos fundamentais relacionados à biologia da célula compatíveis com o currículo de ciências do Ensino Fundamental
Abstract: Considering the importance of cells as the fundamental units of life, they become a central issue in science education, being one of the more complex concepts for student teaching in the beginning of living things study. The student's abstraction difficulty in understanding and contextualizing cells often becomes a problem, because they are accustomed to what is concrete in their daily lives, and cells, because of their size, are only seen with the aid of microscopes. Therefore, to break these orders of magnitude and common sense, it is essential that students understand the importance of the cells also through a historical perspective. Thus, it must be avoided the exclusive study of cell structures and teaching just the scientific results. For this to occur, students must replace their vision of science as a collection of facts and stagnant theories by a comprehension that scientific knowledge is subject to continuous temporal correctness. Currently, several studies mention the relevance and importance of science history in the classroom in various levels of education, which may be an important tool to the teacher that, using updated and appropriate sources, can promote in the students a more critical vision of science and the construction of scientific knowledge. We noticed, in this research, that such contents are poorly present in schools. This is mainly due to the scarcity of available material, both to teachers and schools. In order to overcome this gap, we conducted an extensive literature review of the history of cells discovery, their internal structures and functions, establishing a timeline with several historical episodes that allowed the advancement of knowledge about cells, avoiding a comparative historical view. Then we did a content analysis of the school textbooks best evaluated by the brazilian government PNLD science program (2011/2013), aiming to describe the structure and pattern of distribution of content over cells, highlighting its characteristics and properties. In addition, we seek to describe the units and/or chapter specific to those textbooks that address the history of the discovery and development of studies on cell, seeking to identify the type of historical approach (internalist or externalist) present in these units and/or chapters, when there. In our review of the textbooks we find that there is a great deficiency of contextualized content on cells and its historicity. We believe that an approach that considers a historical perspective, and linking major breakthroughs with technologies present in our daily lives, could contribute significantly to the understanding of the fundamental concepts related to cell biology compatible with the curriculum of elementary school science
Mestrado
Ensino de Ciencias e Matematica
Mestre em Multiunidades em Ensino de Ciências e Matemática
SILVEIRA, LIVIO de B. "Verificacao do comportamento de mastocitos na parede nao mineralizada da bolsa periodontal supra-ossea submetida a radiacao laser de baixa intensidade (Estudo in anima nobile)." reponame:Repositório Institucional do IPEN, 2001. http://repositorio.ipen.br:8080/xmlui/handle/123456789/10864.
Full textMade available in DSpace on 2014-10-09T13:57:55Z (GMT). No. of bitstreams: 1 07162.pdf: 2783402 bytes, checksum: dc83e64aba9a733af3d1707e45c68dcc (MD5)
Dissertacao (Mestrado Profissionalizante em Lasers em Odontologia)
IPEN/D-MPLO
Instituto de Pesquisas Energeticas e Nucleares, IPEN/CNEN-SP; Faculdade de Odontologia, Universidade de Sao Paulo
Wijayasinghe, Athula. "Development and Characterisation of Cathode Materials for the Molten Carbonate Fuel Cell." Doctoral thesis, KTH, Materials Science and Engineering, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3811.
Full textAmong the obstacles for the commercialization of the MoltenCarbonate Fuel Cell (MCFC), the dissolution of thestate-of-the-art lithiated NiO cathode is considered as aprimary lifetime limiting constraint. Development ofalternative cathode materials is considered as a main strategyfor solving the cathode dissolution problem. LiFeO2and LiCoO2had earlier been reported as the most promisingalternative materials; however, they could not satisfactorilysubstitute the lithiated NiO. On the other hand, ternarycompositions of LiFeO2, LiCoO2and NiO are expected to combine some desirableproperties of each component. The aim of this work was todevelop alternative cathode materials for MCFC in the LiFeO2-LiCoO2-NiO ternary system. It was carried out byinvestigating electronic conductivity of the materials, firstin the form of bulk pellets and then in ex-situ sinteredporous-gas-diffusion cathodes, and evaluating theirelectrochemical performance by short-time laboratory-scale celloperations.
Materials in the LiFeO2-NiO binary system and five ternary sub-systems,each with a constant molar ratio of LiFeO2:NiO while varying LiCoO2content, were studied. Powders withcharacteristics appropriate for MCFC cathode fabrication couldbe obtained by the Pechini method. The particle size of LiFeO2-LiCoO2-NiO powders considerably depends on thecalcination temperature and the material composition. Theelectrical conductivity study reveals the ability of preparingLiFeO2-LiCoO2-NiO materials with adequate electricalconductivity for MCFC cathode application.
A bimodal pore structure, appropriate for the MCFC cathode,could be achieved in sintered cathodes prepared usingporeformers and sub-micron size powder. Further, this studyindicates the nature of the compromise to be made between theelectrical conductivity, phase purity, pore structure andporosity in optimization of cathodes for MCFC application. Cellperformance comparable to that expected for the cathode in acommercial MCFC could be achieved with cathodes prepared from20 mole% LiFeO2- 20 mole% LiCoO2- 60 mole% NiO ternary composition. It shows aniR-corrected polarization of 62 mV and a iR-drop of 46 mV at acurrent density of 160 mAcm-2at 650 °C. Altogether, this study revealsthe possibility of preparing LiFeO2-LiCoO2-NiO cathode materials suitable for MCFCapplication.
Keywords: molten carbonate fuel cell (MCFC), MCFC cathode,LiFeO2-LiCoO2-NiO ternary compositions, electrical conductivity,porous gas diffusion electrodes, polarization, electrochemicalperformance, post-cell characterization.
Brown, Jennifer L. "A Molecular and Immunological Investigation of Cellular Responses to Dengue Virus: Identification of Potentially Upregulated Host Genes and the Construction of a Vaccinia Virus Expressing the Dengue 1 Hawaii NS3 Protein." Digital WPI, 2000. https://digitalcommons.wpi.edu/etd-theses/187.
Full text