Relevant bibliographies by topics / NSO cells

Academic literature on the topic 'NSO cells'

Author: Grafiati
Published: 10 December 2022
Last updated: 28 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Contents

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'NSO cells.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "NSO cells"

1

Hosseini, Azar, Vafa Baradaran Rahimi, Hassan Rakhshandeh, and Vahid Reza Askari. "Nigella sativa Oil Reduces LPS-Induced Microglial Inflammation: An Evaluation on M1/M2 Balance." Evidence-Based Complementary and Alternative Medicine 2022 (June 14, 2022): 1–11. http://dx.doi.org/10.1155/2022/5639226.

Full text
Abstract:
Objectives. The immune system plays a critical defence role against infections, injuries, and carcinogenic stimuli. As the macrophages of the brain resides in the innate immune system, microglia and their polarisation (M1/M2) play regulatory roles in inflammation in CNS, such as Parkinson’s, Alzheimer’s, dementia complex, and multiple sclerosis. Nigella sativa belongs to the Ranunculaceae family and has different anti-inflammatory and antioxidant effects. We conducted this study to evaluate the anti-inflammatory and protective properties of N. sativa oil (NSO) on the microglial cells and their polarisation (M1/M2) in the presence of LPS as a model of neuroinflammation. Methods. The protective effects of NSO (10–40 µg/ml) were studied on the LPS-induced microglial cells, and the levels of tumour necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, prostaglandin E2 (PGE2), and IL-10 were evaluated using both ELISA and gene expression methods. The levels of cyclooxygenase-2 (COX-2), inducible NOS (iNOS), and arginase-1 (Arg1) were also evaluated using the real-time PCR method. In addition, nitrite oxide (NO) and urea were measured using biochemical methods. Results. NSO decreased LPS-induced toxicity at all doses ( P < 0.001). NSO (10–40 μg/ml) also significantly reduced the levels of TNF-α, PGE2, IL-1β, and IL-6 in the presence of LPS ( P < 0.01 to 0.001). Pretreatment with NSO attenuated the levels of iNOS but increased Arg1 ( P < 0.001). The ratio of iNOS/Arg1 was also decreased in the presence of NSO ( P < 0.001) than that of the LPS group ( P < 0.001). Conclusion. NSO attenuated LPS-induced inflammation and increased microglia’s anti-inflammatory status. These results may prove that NSO is potentially an immunomodulator for various neurodegenerative diseases by M1 phenotype dominancy, such as Alzheimer’s and Parkinson’s diseases.
APA, Harvard, Vancouver, ISO, and other styles
2

Gvozdeva, Olga V., Alexey A. Belogurov, Ekaterina S. Kuzina, Alexander G. Gabibov, Mariya I. Meschaninova, Alya G. Ven'yaminova, Marina A. Zenkova, Valentin V. Vlassov, and Elena L. Chernolovskaya. "Modified siRNA effectively silence inducible immunoproteasome subunits in NSO cells." Biochimie 125 (June 2016): 75–82. http://dx.doi.org/10.1016/j.biochi.2016.02.015.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Rizk, Fatma H., Marwa A. A. Ibrahim, Marwa M. Abd-Elsalam, Nema A. Soliman, and Sherief M. Abd-Elsalam. "Gastroprotective effects of montelukast andNigella sativaoil against corticosteroid-induced gastric damage: they are much more than antiasthmatic drugs." Canadian Journal of Physiology and Pharmacology 95, no. 6 (June 2017): 714–20. http://dx.doi.org/10.1139/cjpp-2016-0374.

Full text
Abstract:
Corticosteroids are used to treat a variety of diseases like bronchial asthma. However, long-term corticosteroids have a gastric ulcerogenic potential. Montelukast (MTK) and Nigella sativa oil (NSO) are used in treatment of bronchial asthma. Previous studies showed that MTK and NSO had gastroprotective effects in other models of gastric ulcer. The present study assesses synergistic gastroprotective effects of both drugs in dexamethasone (DXM)-induced gastric damage. Fifty male rats were divided into 5 groups: normal control (I), DXM group (II), MTK + DXM group (III), NSO + DXM group (IV), MTK + NSO + DXM group (V). After 7 days, stomachs were removed for biochemical analysis and histological examinations. Significant increases in malondialdehyde (MDA) level, superoxide dismutase (SOD) activity, myeloperoxidase (MPO) activity, and proliferating cell nuclear antigen (PCNA) positive cells, with significant decreases in mucus secretion were detected in DXM-treated group compared with group I. Meanwhile, significant decreases of MDA level, MPO activity, and PCNA positive cells and significant increases in mucus secretion were detected in treated groups compared with group II. SOD activity significantly decreased in group V compared with group II. We could conclude that administration of either MTK or NSO or both with DXM counteracts DXM-induced gastric lesions.
APA, Harvard, Vancouver, ISO, and other styles
4

Madkour, DA, MM Ahmed, SH Orabi, M. Alkafafy, R. Korany, and HK Khalifa. "Emamectin Benzoate-Induced Hepatotoxicity in Rats with Special Reference to Protective Potential of Nigella sativa Oil." Journal of the Hellenic Veterinary Medical Society 73, no. 3 (November 9, 2022): 4607–18. http://dx.doi.org/10.12681/jhvms.28100.

Full text
Abstract:
This study was designed to explore the hepatotoxicity of emamectin in male rats and the possible effect of Nigella sativa oil (NSO) in ameliorating this. Twenty-eight male rats were used in this study. They were divided into four groups, Control group: rats orally administered distilled water; NSO group: rats administered NSO orally; EMB group: rats administered emamectin benzoate orally; and EMB+NSO group: rats orally co-administered NSO with EMB, with the administrations being performed every other day for 6 weeks. Body weight was measured, liver alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) activities were determined, and total protein and albumin levels were recorded. Histopathological examination of the liver was also performed, along with caspase-3 and TNF-α immunostaining of liver tissue. EMB treatment resulted in decreased body weight, while the co-administration of NSO modulated the EMB-induced alterations in body weight. There were also increases in the activities of serum ALT, AST, and ALP and decreases in total protein and albumin levels in the EMB group. Co-treatment with NSO significantly reduced serum ALT, AST, and ALP and improved total protein and albumin levels. Histopathological examination of the liver in the EMB group revealed the presence of different histopathological alterations that were improved by the co-administration of NSO. Immunostaining of caspase-3 and TNF-α in the liver revealed strong expression in the EMB-treated group. Meanwhile, the EMB+NSO group showed weak positivity for immunoreactive cells.
APA, Harvard, Vancouver, ISO, and other styles
5

Hammouda, Souha, Imen Ghzaiel, Pol Picón-Pagès, Wiem Meddeb, Wided Khamlaoui, Sonia Hammami, Francisco J. Muñoz, Mohamed Hammami, and Amira Zarrouk. "Nigella and Milk Thistle Seed Oils: Potential Cytoprotective Effects against 7β-Hydroxycholesterol-Induced Toxicity on SH-SY5Y Cells." Biomolecules 11, no. 6 (May 27, 2021): 797. http://dx.doi.org/10.3390/biom11060797.

Full text
Abstract:
Oxysterols are assumed to be the driving force behind numerous neurodegenerative diseases. In this work, we aimed to study the ability of 7β-hydroxycholesterol (7β-OHC) to trigger oxidative stress and cell death in human neuroblastoma cells (SH-SY5Y) then the capacity of Nigella sativa and Milk thistle seed oils (NSO and MTSO, respectively) to oppose 7β-OHC-induced side effects. The impact of 7β-OHC, associated or not with NSO or MTSO, was studied on different criteria: cell viability; redox status, and apoptosis. Oxidative stress was assessed through the intracellular reactive oxygen species (ROS) production, levels of enzymatic and non-enzymatic antioxidants, lipid, and protein oxidation products. Our results indicate that 7β-OHC (40 µg/mL) exhibit pr-oxidative and pro-apoptotic activities shown by a decrease of the antioxidant enzymatic activities and an increase of ROS production, lipid, and protein oxidation end products as well as nitrotyrosine formation and caspase 3 activation. However, under the pre-treatment with NSO, and especially with MTSO (100 µg/mL), a marked attenuation of oxidative damages was observed. Our study suggests harmful effects of 7β-OHC consisting of pro-oxidative, anti-proliferative, and pro-apoptotic activities that may contribute to neurodegeneration. NSO and especially MTSO showed potential cytoprotection against the cytotoxicity of 7β-OHC.
APA, Harvard, Vancouver, ISO, and other styles
6

Mosbah, Rachid, Mokhtar Ibrahim Yousef, Francesca Maranghi, and Alberto Mantovani. "Protective role of Nigella sativa oil against reproductive toxicity, hormonal alterations, and oxidative damage induced by chlorpyrifos in male rats." Toxicology and Industrial Health 32, no. 7 (November 25, 2014): 1266–77. http://dx.doi.org/10.1177/0748233714554675.

Full text
Abstract:
This study is aimed at elucidating the possible protective effects of Nigella sativa oil (NSO) in alleviating the toxicity of chlorpyrifos (CPF) on reproductive performance in male rats. Animals were orally administered with NSO (1 ml/kg/day), CPF (20 mg/kg/day), and NSO + CPF every day for 4 weeks. Results showed that CPF decreased spermatid number, sperm count, daily sperm production, and sperm motility while increased dead sperm and abnormal sperm compared with the control. Also the levels of testosterone, thyroxine levels, steroidogenic enzyme 17-ketosteroid reductase, body weight, food intake, and relative weight of reproductive organs were decreased. Thiobarbituric acid reactive substances were increased, while glutathione (GSH) and antioxidant enzymes were decreased in plasma and testes of rats treated with CPF. Histopathological examination of testes showed a decrease in the number of seminiferous tubules, form shrinkage, enlargement of the connective tissue and gametogenic changes in germ cells of rats treated with CPF. NSO alone increased testosterone, semen characteristics, GSH, and antioxidant enzymes and decreased the levels of free radicals. Furthermore, the presence of NSO with CPF alleviates its toxic effects. Our results indicated that NSO can improve semen picture and moderate CPF-induced reproductive toxicity.
APA, Harvard, Vancouver, ISO, and other styles
7

Peakman, Timothy C., Jennifer Worden, Robert H. Harris, Helen Cooper, John Tite, Martin J. Page, Dirk R. Gewert, Michelle Bartholemew, James S. Crowe, and Sara Brett. "Comparison of expression of a humanized monoclonal antibody in mouse NSO myeloma cells and Chinese Hamster Ovary cells." Human Antibodies 5, no. 1-2 (March 1, 1994): 65–74. http://dx.doi.org/10.3233/hab-1994-51-209.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Imam, Aminu, Christianah Oyegbola, Maryam Busari, Rukayat Jaji-Sulaimon, Abdulmusawwir Alli-Oluwafuyi, Akeem Okesina, Adam Afodun, and Moyosore Ajao. "Nigella sativa Oil Preserved Anxiety-Like, Motor and Memory Related Behaviours and Neuronal Integrity in Dichlorvos Induced Acetyl Cholinesterase Inhibitions in Rats." NIgerian Journal of Neuroscience 12, no. 3 (December 31, 2021): 84–91. http://dx.doi.org/10.47081/njn2021.12.3/002.

Full text
Abstract:
Organophosphates are irreversible cholinesterase (ChE) inhibitors with neurological consequences, and there is not yet an effective antidote. Here, we investigated the effects of Nigella sativa oil (NSO) on the ChE inhibition, neurobehavioural and histopathological changes following dichlorvos (DDVP) ingestions in rats. Thirty-two male Wistar rats were randomised into four groups, receiving 1 ml/kg of normal saline, 8.8 mg/kg of DDVP, 8.8 mg/kg of DDVP and 1 ml/kg of NSO, and 1 ml/kg of NSO only respectively, for 14 consecutive days. Locomotor, anxiety-like behaviours and spatial working memory were assessed on the 14th day, using open field (OF), Y-maze and modified elevated plus maze paradigms. The rats were euthanized on the 15th day and the brains excised; three brains were fixed for histopathology, and the other five prepared for biochemical analysis of acetyl cholinesterase (AChE). DDVP exposure caused significant reductions in frontal, amygdala and cerebella AChE activity, spontaneous alternations, line crossing and rearing frequencies and time in centre square, and caused increase in freezing period, transfer latency and necrotic-like cells. NSO intervention was able to reverse DDVP effects on AChE activities, explorative, locomotor, anxiety and spatial memory behaviours in co-exposed rats. It also preserved the histological integrity of the investigated brain regions. It can be concluded that NSO, may be potent against organophosphates induced neurotoxicity and their neurobehavioural consequences through the modulation of AChE activities.
APA, Harvard, Vancouver, ISO, and other styles
9

Barlianto, Wisnu, Maria Rachmawati, Muhammad Irawan, and Desy Wulandari. "Effects of Nigella sativa oil on Th1/Th2, cytokine balance, and improvement of asthma control in children." Paediatrica Indonesiana 57, no. 5 (October 31, 2017): 223. http://dx.doi.org/10.14238/pi57.5.2017.1399.

Full text
Abstract:
Background Asthma is a chronic inflammatory disease of the airways characterized by involvement of a variety of inflammatory cells. Asthma is associated with imbalances between Th1/Th2 cells and their characteristic cytokine profiles. Nigella sativa is a plant that possesses immunomodulatory and anti-inflammatory properties.Objective To investigate the potential anti-asthmatic effect of Nigella sativa oil on Th1/Th2 cells, IFN-ɣ/IL-4 cytokines, and improvement of asthma control.Methods Children aged 6-15 years with asthma in Dr. Saiful Anwar Hospital, Malang, were enrolled in this study. All patients were treated based on standard treatment guidelines for asthma. Nigella sativa oil (NSO) was given per oral as supplementary treatment at a dose of 15-30 mg/kg/day for 8 weeks, in a randomized, single-blind, controlled trial. Peripheral Th1 and Th2 cells were counted by flow cytometry and IFN-ɣ and IL-4 cytokines were measured by ELISA. Improvement of asthma control was assessed by the asthma control test (ACT) score.Results Twenty-eight patients completed the study, 14 in the NSO treatment group and 14 in standard treatment group. No significant differences were found in the number of Th1 and Th2 cells, or in the Th1/Th2 ratio between groups after treatment (P=0.074, P=0.481, and P=0.265, respectively). Compared to the control, the NSO group showed a significant elevation of IFN-ɣ (P=0.046) and reduction of IL-4 (P=0.002). At the end of study, ACT score was not significantly different between groups (P=0.413).Conclusion Supplementation with Nigella sativa oil improves IFN-ɣ/IL-4 balance and asthma control in children with asthma.
APA, Harvard, Vancouver, ISO, and other styles
10

Barlianto, Wisnu, Maria Rachmawati, Muhammad Irawan, and Desy Wulandari. "Effects of Nigella sativa oil on Th1/Th2, cytokine balance, and improvement of asthma control in children." Paediatrica Indonesiana 57, no. 5 (January 5, 2018): 223. http://dx.doi.org/10.14238/pi57.5.2017.223-8.

Full text
Abstract:
Background Asthma is a chronic inflammatory disease of the airways characterized by involvement of a variety of inflammatory cells. Asthma is associated with imbalances between Th1/Th2 cells and their characteristic cytokine profiles. Nigella sativa is a plant that possesses immunomodulatory and anti-inflammatory properties.Objective To investigate the potential anti-asthmatic effect of Nigella sativa oil on Th1/Th2 cells, IFN-ɣ/IL-4 cytokines, and improvement of asthma control.Methods Children aged 6-15 years with asthma in Dr. Saiful Anwar Hospital, Malang, were enrolled in this study. All patients were treated based on standard treatment guidelines for asthma. Nigella sativa oil (NSO) was given per oral as supplementary treatment at a dose of 15-30 mg/kg/day for 8 weeks, in a randomized, single-blind, controlled trial. Peripheral Th1 and Th2 cells were counted by flow cytometry and IFN-ɣ and IL-4 cytokines were measured by ELISA. Improvement of asthma control was assessed by the asthma control test (ACT) score.Results Twenty-eight patients completed the study, 14 in the NSO treatment group and 14 in standard treatment group. No significant differences were found in the number of Th1 and Th2 cells, or in the Th1/Th2 ratio between groups after treatment (P=0.074, P=0.481, and P=0.265, respectively). Compared to the control, the NSO group showed a significant elevation of IFN-ɣ (P=0.046) and reduction of IL-4 (P=0.002). At the end of study, ACT score was not significantly different between groups (P=0.413).Conclusion Supplementation with Nigella sativa oil improves IFN-ɣ/IL-4 balance and asthma control in children with asthma.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "NSO cells"

1

Sage, Elizabeth Ann. "The functional competence of animal cells in culture : the NSO cell proteome." Thesis, University of Kent, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.399618.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Duncan, Philippa Jane. "The effect of hyperosmotic conditions on growth and recombinant protein production by NSO myeloma cells in culture." Thesis, Liverpool John Moores University, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.403285.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Osman, Jason John. "Response of GS-NSO mouse myeloma cells to pH fluctuations relevant to those found in large scale fermentation." Thesis, University of Reading, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.393530.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Waskow, Claudia, Bonin Malte von, Martin Wermke, Cosgun Kadriye Nehir, Christian Thiede, Martin Bornhauser, and Gerard Wagemaker. "In Vivo Expansion of Co-Transplanted T Cells Impacts on Tumor Re-Initiating Activity of Human Acute Myeloid Leukemia in NSG Mice." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2016. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-191633.

Full text
Abstract:
Human cells from acute myeloid leukemia (AML) patients are frequently transplanted into immune-compromised mouse strains to provide an in vivo environment for studies on the biology of the disease. Since frequencies of leukemia re-initiating cells are low and a unique cell surface phenotype that includes all tumor re-initiating activity remains unknown, the underlying mechanisms leading to limitations in the xenotransplantation assay need to be understood and overcome to obtain robust engraftment of AML-containing samples. We report here that in the NSG xenotransplantation assay, the large majority of mononucleated cells from patients with AML fail to establish a reproducible myeloid engraftment despite high donor chimerism. Instead, donor-derived cells mainly consist of polyclonal disease-unrelated expanded co-transplanted human T lymphocytes that induce xenogeneic graft versus host disease and mask the engraftment of human AML in mice. Engraftment of mainly myeloid cell types can be enforced by the prevention of T cell expansion through the depletion of lymphocytes from the graft prior transplantation.
APA, Harvard, Vancouver, ISO, and other styles
5

Yu, Yi-Hsin Biotechnology &amp Biomolecular Sciences Faculty of Science UNSW. "Role of the RNAi pathway in influenza a virus infected mammalian cells." Publisher:University of New South Wales. Biotechnology & Biomolecular Sciences, 2008. http://handle.unsw.edu.au/1959.4/41545.

Full text
Abstract:
The interferon (lFN) signalling and RNA interference (RNAi) pathways are the major antiviral pathways in animals and plants, respectively. Although the mechanism of RNAi remains to be completely characterised, the genes that encode the proteins involved in this process have been identified in the plant, fungi and animal kingdoms (Fagard et al., 2000, Grishok et aI., 2000, Hall et al., 2003, Kanellopoulou et al., 2005, Kolb et al., 2005); with comparative analyses indicating that RNAi is an evolutionarily conserved mechanism. Several studies have identified RNAi suppressors encoded by animal viruses, suggesting an antiviral role for the RNAi pathway in animals as well as plants (Andersson et al., 2005, Bennasser et al., 2006, Garcia et al., 2006, Li et al., 2004, Lichner et al., 2003, Lingel et al., 2005, Lu & Cullen, 2004, Wang et al., 2006). However, most of these studies were performed in non-mammalian systems and as yet, there is no direct evidence indicating that the RNAi pathway plays a significant antiviral role during the infection of mammalian cells. Interestingly, several viruses have now been shown to express their own microRNA (miRNA) in infected cells (Grey et al., 2005, Pfeffer et al., 2005, Pfeffer et al., 2004, Samols et al., 2005, Sullivan et al., 2005). Further, in the case of hepatitis C virus (HCV), there is evidence that the virus usurps the host cell miRNAs to enhance viral replication (Jopling et al., 2005). The principal aim of this project was to investigate the role of RNAi in mammalian cells during viral infection, particularly infection with the influenza A virus. This thesis is divided into six major chapters followed by a brief general discussion. Chapter 1 contains a general introduction to the RNAi pathway. It describes the history of the discovery of RNAi and summarizes the known and proposed antiviral roles of the RNAi pathway in plants and mammalian cells. Chapter 2 describes the general materials and methods used for this project. There are four main result chapters, each dealing with a specific experimental system. Each chapter is divided into a brief introduction, specific materials and methods used, followed by presentation of the experimental results and a brief discussion. Chapter 3 describes the development of an in vitro Dicer activity assay to study the effect of viral proteins on the activity of the mammalian Dicer protein. It was demonstrated that crude cell lysates derived from influenza A virus infected cells impaired the activity of Dicer and this observation was not due to degradation of the Dicer protein by virally-induced proteases. Chapter 4 describes the use of a GFP reporter assay for screening potential RNAi suppressors. This assay is suitable for studying viral proteins in isolation. The effect of the influenza NS1 protein on the RNAi pathway in HEK293 cells was investigated and it was shown that NS1 could exert modest, but nevertheless significant, suppression of the RNAi pathway. Northern studies, performed to examine the processing of shRNA in the presence of NS1, demonstrated that NSI suppressed the RNAi mechanism through interfering with the maturation ofshRNA into siRNA. Chapter 5 describes the effect of over-expressing components of the RNAi pathway on influenza A virus infection. In these experiments, Exportin 5, which encodes a protein involved in the transport of pre-miRNA/shRNA into the cytoplasm, was over-expressed during influenza A virus infection. Reduced viral infection was observed in cells over-expressing Exportin 5, suggesting that this treatment protects cells from virus infection. Chapter 6 describes the expressed small RNA profile during influenza A virus infection in MDCK cells. Novel canine miRNA homologues were identified through cloning and sequencing. No definitive evidence for virally-derived siRNA/miRNA was found but a general reduction of endogenous miRNA expression was detected.
APA, Harvard, Vancouver, ISO, and other styles
6

Waskow, Claudia, Bonin Malte von, Martin Wermke, Cosgun Kadriye Nehir, Christian Thiede, Martin Bornhauser, and Gerard Wagemaker. "In Vivo Expansion of Co-Transplanted T Cells Impacts on Tumor Re-Initiating Activity of Human Acute Myeloid Leukemia in NSG Mice." Public Library of Science, 2013. https://tud.qucosa.de/id/qucosa%3A28097.

Full text
Abstract:
Human cells from acute myeloid leukemia (AML) patients are frequently transplanted into immune-compromised mouse strains to provide an in vivo environment for studies on the biology of the disease. Since frequencies of leukemia re-initiating cells are low and a unique cell surface phenotype that includes all tumor re-initiating activity remains unknown, the underlying mechanisms leading to limitations in the xenotransplantation assay need to be understood and overcome to obtain robust engraftment of AML-containing samples. We report here that in the NSG xenotransplantation assay, the large majority of mononucleated cells from patients with AML fail to establish a reproducible myeloid engraftment despite high donor chimerism. Instead, donor-derived cells mainly consist of polyclonal disease-unrelated expanded co-transplanted human T lymphocytes that induce xenogeneic graft versus host disease and mask the engraftment of human AML in mice. Engraftment of mainly myeloid cell types can be enforced by the prevention of T cell expansion through the depletion of lymphocytes from the graft prior transplantation.
APA, Harvard, Vancouver, ISO, and other styles
7

Wu, Mon-Han. "The effect of osmotic pressure on GS-NSO antibody production cell line." Thesis, Imperial College London, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439532.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Chen, Baiyu, and 陳白羽. "Suprachiasmatic nucleus projecting retinal ganglion cells in golden hamsters development, morphology and relationship with NOS expressingamacrine cells." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2006. http://hub.hku.hk/bib/B37238218.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Long, Xiangtian. "Synthesis and Evaluation of Stimulatory Properties of Glycolipids for Natural Killer T Cells." BYU ScholarsArchive, 2009. https://scholarsarchive.byu.edu/etd/2133.

Full text
Abstract:
Natural killer T cells (NKT cells) are a subset of T cells. They regulate a wide range of diseases including infection, tumor growth, and autoimmune diseases, through recognizing glycolipid antigens in the context of CD1d. An understanding of the scope of glycolipid antigens would facilitate use of this cell type in controlling immune responses. Till today, a lysosomal glycolipid, isoglobotrihexosylceramide (iGb3), is the only natural glycolipid that has been found to be recognized by both human and mouse NKT cells. To elucidate the molecular basis of this specific recognition, iGb3 variants were designed and prepared: i) replacement of the C26 acyl chain with shortened acyl chains; ii) replacement of the distal galactose with glucose and mannose; iii) replacement of the intermediate galactose with glucose; iv) replacement of the proximal glucose with galactose. Among these glycolipids, the iGb3 variants with shortened acyl chains are potent stimulators of NKT cells. The iGb3 variant with intermediate glucose also showed the ability to stimulate NKT cells, but this finding needs to be verified. Our findings support the specific recognition of iGb3 by NKT cells. The search for other natural glycolipid antigens focuses on glycolipids that are isolated from bacteria and parasites. Recently, glycosphingolipids (GSL-1, -3, and -4) isolated from the sphingomonodaceae family of bacteria were characterized. GSL-1 has been shown to be a potent stimulator of NKT cells. Moreover, it has been reported that GSL-4 is a stimulator as well. To verify the structures and stimulatory properties of GSLs, GSL-1 to -4 were prepared and tested for their abilities to stimulate NKT cells. The result that only GSL-1 can stimulate NKT cells suggests that synthesis of these higher order GSLs would be an immune evasion mechanism. Neutral glycosphingolipids from sheep-derived F. hepatica liver flukes, a causative agent of fascioliasis, were isolated and characterized. Their structures are closely related to iGb3. Among these glycolipids, neo-iGb4s could be truncated to iGb3 in the lysosome and thus stimulate NKT cells. To test this hypothesis, these glycosphingolipids were prepared and tested. None of these synthetic glycolipids stimulates NKT cells, which suggests that the secretion of these glycolipids by F. hepatica could be the result of the parasite-immune-evasion mechanism.
APA, Harvard, Vancouver, ISO, and other styles
10

Chen, Baiyu. "Suprachiasmatic nucleus projecting retinal ganglion cells in golden hamsters development, morphology and relationship with NOS expressing amacrine cells." Click to view the E-thesis via HKUTO, 2006. http://sunzi.lib.hku.hk/hkuto/record/B37238218.

Full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Books on the topic "NSO cells"

1

Marcus Cornelis Maris van Dijk. Recognition and processing of apolipoprotein E-rich (neo)lipoproteins) by liver cells. [Netherlsnds: s.n.], 1992.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
2

Bach, Johann Sebastian. Cello suites nos. 1, 2, and 3, for guitar. New York: International Music Co., 1999.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
3

Ligeti, György. String quartets nos. 1 & 2 ; Ramifications ; Cello sonata ; Melodien. Hamburg: Deutsche Grammophon,c2003, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
4

Joëlle, Menrath, ed. Mobile attitude: Ce que les portables ont changé dans nos vies. Paris: Hachette, 2005.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
5

Girard, Serge. L'au-delà à l'écoute de nos prières: La prière des vivants et celle des morts. Chicoutimi, Québec: Éditions JCL, 1997.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
6

Settipani, Christian. Nos ancêtres de l'Antiquité: Études des possibilités de liens généalogiques entre les familles de l'Antiquité et celles du haut Moyen-Age européen. Paris: Éditions Christian, 1991.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
7

Settipani, Christian. Nos ancêtres de l'Antiquité: Études des possibilités de liens généalogiques entre les familles de l'Antiquité et celles du haut Moyen-Age européen. Paris: Editions Christian, 1991.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
8

Algoud, François Marie. Histoire de la volonté de perversion de l'intelligence et des mœurs: Du XVIe siècle à nos jours : les oppositions à celle-ci ; précédée de, Tout se tient, vers Dieu ou vers la bête? Chiré-en-Montreuil: Editions de Chiré, 1996.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
9

Miller, Ada. Nos separan los celos. Debolsillo, 2003.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
10

Miller, Ada. Nos Separan Los Celos. Plaza & Janes, 2005.

Find full text
APA, Harvard, Vancouver, ISO, and other styles
More sources

Book chapters on the topic "NSO cells"

1

Bukovsky, Antonin, and Michael R. Caudle. "Mammalian Neo-Oogenesis from Ovarian Stem CellsIn VivoandIn Vitro." In Cell and Molecular Biology and Imaging of Stem Cells, 67–136. Hoboken, New Jersey: John Wiley & Sons, Inc, 2014. http://dx.doi.org/10.1002/9781118285602.ch4.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Iyer, Anand Krishnan V., Neelam Azad, Liying Wang, and Yon Rojanasakul. "S-Nitrosylation – How Cancer Cells Say NO to Cell Death." In Nitric Oxide (NO) and Cancer, 85–102. New York, NY: Springer New York, 2010. http://dx.doi.org/10.1007/978-1-4419-1432-3_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Swiderek, Halina, Anna Logan, and Mohamed Al-Rubeai. "Transcriptomic Analysis of Antibody Producing NS0 Cell Line Under Hypothermic and Hypoxic Conditions." In Cells and Culture, 157–60. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_25.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Ray, Nitya G., Roberto Rivera, Rajeew Gupta, and Dale Mueller. "Large-Scale Production of Humanized Monoclonal Antibody Expressed in a GS-NSO Cell Line." In Animal Cell Technology, 235–41. Dordrecht: Springer Netherlands, 1997. http://dx.doi.org/10.1007/978-94-011-5404-8_37.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Khoo, Soo Hean Gary, and Mohamed Al-Rubeai. "Towards a Systems-Level Understanding of Increased Specific Productivity in Proliferation Arrested Myeloma NS0 Cells." In Cells and Culture, 425–28. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_73.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

Krampe, Britta, Halina Swiderek, and Mohamed Al-Rubeai. "Transcriptomic and Proteomic Analysis of Antibody Producing NS0 Cells Cultivated at Different Cell Densities in Perfusion Culture." In Cells and Culture, 145–49. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-3419-9_23.

Full text
APA, Harvard, Vancouver, ISO, and other styles
7

Vasylyev, Oleksandr, Iegor Brodnikovskyi, Mykola Brychevskyi, and Ievhenii Pryshchepa. "NiO-10Sc1CeSZ Anode: Structure and Mechanical Behavior." In Advances in Solid Oxide Fuel Cells III, 361–76. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2009. http://dx.doi.org/10.1002/9780470339534.ch33.

Full text
APA, Harvard, Vancouver, ISO, and other styles
8

Pfeilschifter, Josef, and Heiko Mühl. "NOS in Mesangial Cells: Physiological and Pathophysiological Roles." In Nitric Oxide and the Kidney, 198–215. Boston, MA: Springer US, 1997. http://dx.doi.org/10.1007/978-1-4615-6039-5_10.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Watanabe, Shikiko, John Shuttleworth, and Mohamed Al-Rubeai. "Regulation of Cell Cycle and Productivity in NS0 Cells by the Over-Expression of p21CIP1." In Animal Cell Technology: From Target to Market, 149–55. Dordrecht: Springer Netherlands, 2001. http://dx.doi.org/10.1007/978-94-010-0369-8_31.

Full text
APA, Harvard, Vancouver, ISO, and other styles
10

Baluška, František, and Sherrie Lyons. "Symbiotic Origin of Eukaryotic Nucleus: From Cell Body to Neo-Energide." In Plant Cell Monographs, 39–66. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-69944-8_3.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Conference papers on the topic "NSO cells"

1

Rana, Seema, and Rajiv Tangri. "Anaplastic large cell lymphoma ALK negative vs. peripheral T cell lymphoma (NOS) - diagnostic dilemma." In 16th Annual International Conference RGCON. Thieme Medical and Scientific Publishers Private Ltd., 2016. http://dx.doi.org/10.1055/s-0039-1685354.

Full text
Abstract:
Middle aged female presented with generalised lymphadenopathy and fever for last one month. Peripheral blood findings were within normal limits. There was no extra nodal involvement. FNAC performed initially from a cervical node suggested possibility of Hodgkin’s lymphoma and a whole node biopsy was performed. Histopathogical examination revealed effaced nodal architecture and a polymorphous population of lymphocytes, plasma cells, neutrophils and scattered large mononuclear cells with prominent nucleolus. An initial panel of CD3, CD20, LCA, CD15, CD30 and PAX5 was performed. The large atypical cells were positive for LCA, CD3 and CD30 with variable positivity for CD15. CD 30 showed Golgi and membranous staining. These large atypical cells were negative for PAX5 and CD20. In view of above findings, Hodgkin’s lymphoma was ruled out and a possibility of Non- Hodgkin’s lymphoma was considered. Further IHC markers were performed which included CD2, CD5, CD7, EMA, Alk, CD10 and KI67. CD5 showed variable positivity. The cells of interest were negative for CD2, CD7, ALK and EMA. Ki 67 index was 70-80%. Overall histological and IHC findings favoured Alk negative Anaplastic large cell lymphoma. Differential diagnosis considered was peripheral T cell lymphoma (NOS). Hodgkin’s lymphoma, peripheral T cell lymphoma (NOS) and anaplastic large cell lymphoma share common histomorphological findings. With careful analysis of Immunohistochemistry, it is easier to categorise Hodgkin’s lymphoma. ALK negative anaplastic large cell lymphoma and peripheral T cell lymphoma (NOS) are difficult to categorise and show overlapping features. We in this case have discussed clinical, histomorphological and IHC pattern of Alk negative Anaplastic large cell lymphoma.
APA, Harvard, Vancouver, ISO, and other styles
2

Kuttikrishnan, Shilpa, Kirti S. Prabhu, Tamam Elimat, Ashraf Khalil, Nicholas H. Oberlies, Feras Q. Alali, and Shahab Uddin. "Anticancer Activity of Neosetophomone B, An Aquatic Fungal Secondary Metabolite, Against Hematological Malignancie S." In Qatar University Annual Research Forum & Exhibition. Qatar University Press, 2021. http://dx.doi.org/10.29117/quarfe.2021.0106.

Full text
Abstract:
Cancer is one of the most life threatening diseases, causing nearly 13% death in the worldwide. Leukemia, cancer of the hematopoetic cells is the main cause of cancer death in adults and children. Therapeutic agents used in treatment of cancer are known to have narrow therapeutic window and tendency to develop resistance against some cancer cell lines thus, proposing a need to discover some novel agents to treat cancer. In the present study we investigated the anticancer activity of Neosetophomone B(NSP-B), an aquatic fungal metabolite isolated from Neosetophoma sp against leukemic cells (K562 and U937). MTT results demonstrated a dose dependent inhibition of cell proliferation in K562 and U937 cell lines. Annexin staining using flow cytometry indicated that NSP-B treatment cause a dose dependent apoptosis in leukemic cells.Western blot analysis showed that NSP-B mediated apoptosis involves sequential activation of caspase 9, 3 and poly (ADP-ribose) polymerase (PARP) cleavage. Furthermore NSP-B treatment of leukemic cells resulted in upregulation of pro-apoptotic proteins (Bax) with downregulation of anti-apoptotic proteins ( Bcl-2 ).Thus, present study focuses on exploring the mechanism of anticancer activity of NSP-B on leukemic cells, raising the possibility of its use as a novel therapeutic agent for hematological malignancies. Results: We sought to determine whether NSP-B suppresses the growth of leukemic cell lines. We tested a panel of leukemic cell lines with different doses of NSP-B. Cell viability decreased in a concentration-dependent manner in K562 and U937 cell lines. NSP-B induced apoptosis in K562 and U937 cell lines via downregulation of anti-apoptotic proteins and enhancement of pro-apoptotic proteins. NSP-B induced the activation of caspase cascade signaling pathway. Altogether our results suggest that the NSP-B plays an important role in apoptosis in leukemic cell lines .Conclusions: Our data provides insight on anticancer activities of NSP-B in leukemic cell lines (K562 and U937). NSP-B inhibit cell viability via inducing apoptosis. The NSP-B mediated apoptosis occurs via downregulation of anti-apoptotic proteins and enhancement of pro-apototic proteins, thereby activating the caspase-cascade signaling. Further studies are required to elicit role of NSP-B in regulating molecular pathway involved in the progression of cancer. Taken together, above results suggest that NSP-B may have a future therapeutic role in leukemia and possibly other hematological malignancies.
APA, Harvard, Vancouver, ISO, and other styles
3

Slomka, Noa, and Amit Gefen. "Cell-to-Cell Variability in Tensile Strains Occurring in the Plasma Membrane and Nuclear Surface Area of Compressed Myoblasts." In ASME 2011 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2011. http://dx.doi.org/10.1115/sbc2011-53234.

Full text
Abstract:
Confocal-microscopy-based three-dimensional (3D) cell-specific finite element (FE) modeling has recently been introduced by our group as a method to simulate the structural behavior of realistic cell geometries under external loading, while considering details of intracellular organelle [1,2]. This method provides comprehensive knowledge regarding cellular mechanics problems, for example, it is useful in the context of understanding the aetiology of deep tissue injury (DTI) — a type of a serious pressure ulcer associated with sustained cellular deformations [3–6]. In this regard, we previously postulated that sustained deformations of soft tissues near bony prominences could cause cell death by a mechanism of locally stretching cells, the consequence of which being that the permeability of the plasma membrane and nuclear surface area (NSA) in the affected cells increases. This, in turn, pathologically changes cell-matrix and intracellular transport profiles and eventually disrupts cellular homeostasis [1,7]. We hypothesize that tensile strains in the plasma membrane and NSA might differ in magnitude and pattern across externally-loaded individual cells of the same cell type, due to cell-to-cell morphological differences. Hence, in this study, we utilize confocal-based cell-specific 3D modeling to analyze tensile strain states in the plasma membrane and NSA of 3 different skeletal muscle cells (myoblasts) subjected to compression. We were specifically interested in chacterizing cell-to-cell variability in magnitudes and patterns of the localized strains.
APA, Harvard, Vancouver, ISO, and other styles
4

Haynes, Comas. "Preliminary Aeronautical Design Considerations of “High NOS” SOFC/GT Hybrids." In ASME 2004 2nd International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2004. http://dx.doi.org/10.1115/fuelcell2004-2533.

Full text
Abstract:
Aerovehicular application of solid oxide fuel cell/gas turbine (SOFC/GT) auxiliary power units (APUs) is a promising concept that may significantly aid the advent of next-generation, environmentally benign aircrafts. The hybrid concept may not only be a novelty, but a necessity, for viably realizing the benefits of fuel cells on-board. A generic design concept is proposed wherein the strategic integration of the cell stack with a bottoming turbine for APU duty, along with regenerative fuel recirculation, could be used to foster larger reactant “numbers-of-stoichs” (NOS) levels within the stack. This may engender larger gravimetric power densities, capacitance for load following and thermal reliability. Fuel utilization/NOS may then be a significant design degree-of-freedom to include within the design process.
APA, Harvard, Vancouver, ISO, and other styles
5

Prakash, Deep, Raja K. Lenka, A. K. Sahu, P. K. Patro, P. K. Sinha, and A. K. Suri. "Effect of Cathode Functional Layer on the Electrical Performance of Tubular Solid Oxide Fuel Cell." In ASME 2010 8th International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2010. http://dx.doi.org/10.1115/fuelcell2010-33352.

Full text
Abstract:
Development of technology related to solid oxide fuel cells at Materials Group, BARC includes synthesis of materials viz. lanthanum strontium manganite (LSM), yttria stabilized zirconia (YSZ), NiO etc. using chemical methods followed by shaping components and integrating into single cells. The indigenously prepared materials are converted to single cells for studies on material characterization and cell performances. This paper presents results of studies on electrical performances of tubular solid oxide fuel cell under hydrogen and oxygen atmospheres and correlates them with the use of cathode functional layers. Studies on symmetrical cells comprising of YSZ electrolyte sandwiched between cathode layers were carried out using electrical impedance spectroscopy (EIS). The EIS results exhibit improvement of area specific resistance (ASR) on incorporation of functional layers. Based on EIS data, double functional layers with varying compositions were incorporated in button cells as well as tubular single cells. Two types of tubular cells were fabricated, electrolyte supported and cathode supported. The electrolyte supported tubular cells were fabricated by making one-end closed tubes of YSZ, followed by coating with porous LSM inside and porous NiO-YSZ layer outside the tubes. On the other hand, tubular cathode supported cells were fabricated by one-end closed porous LSM tubes, followed by subsequent coatings of electrolyte and anode. The cells were characterized for electrical performance from 800 to 1000°C. Electrical power output from electrolyte supported cells with cathode functional layers increased from 300 mW per cell to 500 mW per cell. On the other hand, cathode supported cells exhibited improvement in ASR from ∼ 2 Ωcm2 to &lt; 1 Ωcm2. The results were correlated with microstructure, area of triple phase boundaries and catalytic activity of cathode.
APA, Harvard, Vancouver, ISO, and other styles
6

Sebastine, I. M., and D. J. Williams. "Requirements for the Manufacturing of Scaffold Biomaterial With Features at Multiple Scales." In ASME 2005 International Mechanical Engineering Congress and Exposition. ASMEDC, 2005. http://dx.doi.org/10.1115/imece2005-82515.

Full text
Abstract:
Tissue engineering aims to restore the complex function of diseased tissue using cells and scaffold materials. Tissue engineering scaffolds are three-dimensional (3D) structures that assist in the tissue engineering process by providing a site for cells to attach, proliferate, differentiate and secrete an extra-cellular matrix, eventually leading cells to form a neo-tissue of predetermined, three-dimensional shape and size. For a scaffold to function effectively, it must possess the optimum structural parameters conducive to the cellular activities that lead to tissue formation; these include cell penetration and migration into the scaffold, cell attachment onto the scaffold substrate, cell spreading and proliferation and cell orientation. In vivo, cells are organized in functional tissue units that repeat on the order of 100 μm. Fine scaffold features have been shown to provide control over attachment, migration and differentiation of cells. In order to design such 3D featured constructs effectively understanding the biological response of cells across length scales from nanometer to millimeter range is crucial. Scaffold biomaterials may need to be tailored at three different length scales: nanostructure (&lt;1μm), microstructure (&lt;20–100μm), and macrostructure (&gt;100μm) to produce biocompatible and biofunctional scaffolds that closely resemble the extracellular matrix (ECM) of the natural tissue environment and promote cell adhesion, attachment, spreading, orientation, rate of movement, and activation. Identification of suitable fabrication techniques for manufacturing scaffolds with the required features at multiple scales is a significant challenge. This review highlights the effect and importance of the features of scaffolds that can influence the behaviour of cells/tissue at different length scales in vitro to increase our understanding of the requirements for the manufacture of functional 3D tissue constructs.
APA, Harvard, Vancouver, ISO, and other styles
7

Gilchrist, Christopher L., David S. Ruch, Dianne Little, and Farshid Guilak. "Nano-Scale and Micro-Scale Substrate Architectures Direct Collagen Alignment in Tendon Neo-Tissue Formation." In ASME 2013 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/sbc2013-14474.

Full text
Abstract:
Biomaterial scaffolds that present defined microenvironmental cues (e.g., nano-topography) to cells have shown promise for a variety of tissue engineering applications. However, the specific cues best suited for promoting the formation of aligned, fibrous tissues such as tendon are not fully understood. In this study, we utilize a micro-photopatterning (μPP) model system to precisely arrange scaffold-mimicking microenvironmental cues and investigate their role in the formation of tendon-like neo-tissues. Our data show that scaffold architectural features at both nano- and micro-length scales may be important parameters for directing tendon-like cell organization and the formation of aligned, fibrillar collagen.
APA, Harvard, Vancouver, ISO, and other styles
8

Zhao, Ruogang, Kristine Wyss, and Craig A. Simmons. "Comparison of Three Material Models to Predict the Time-Dependent Deformation of a Single Cell Under Micropipette Aspiration." In ASME 2008 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2008. http://dx.doi.org/10.1115/sbc2008-192940.

Full text
Abstract:
Micropipette aspiration is an experimental technique that is used widely to measure the mechanical properties of single cells [1]. The viscoelastic properties of the probed cell are often estimated by fitting experimental data to a three-parameter standard linear solid (SLS) half-space model (e.g., [1]). However, this analytical model does not account for the large strains that can occur with micropipette aspiration. This limitation has motivated the development of numerical methods to interpret the experimental data. For example, Zhou [2] implemented a material model combining a hyperelastic neo-Hookean material and a viscoelastic SLS material in an axisymmetric finite element (FE) model to simulate large strain micropipette aspiration of a suspended cell. The time-dependent creep deformation of cells has also been described by power-law rheology [3]; this material model has been applied to micropipette aspiration of nuclei [4], but not whole cells.
APA, Harvard, Vancouver, ISO, and other styles
9

Martins, R. F., M. C. Brant, R. Z. Domingues, and T. Matencio. "NiO/YSZ Composites for SOFC: Synthesis and Characterization." In ASME 2006 4th International Conference on Fuel Cell Science, Engineering and Technology. ASMEDC, 2006. http://dx.doi.org/10.1115/fuelcell2006-97146.

Full text
Abstract:
Solid oxide fuel cell (SOFC) works at high temperature and is normally used in stationary devices which are of wide interest in the world market. The most currently SOFC developers utilize yttria-stabilized zirconia (YSZ) as electrolyte, strontium-doped lanthanum manganite (LSM) as cathode and a Ni/YSZ cermet obtained from NiO/YSZ in situ reduction as anode. The electrode performance is directly influenced by powder grain sizes, homogeneity, purity, and amount of Ni. Although physical mixture is a simpler procedure it hardly gives homogeneous materials as suitable to SOFC applications. Alternative chemical methods are sol-gel, impregnation and those derived from Pechini route. The present work compares thermal stability and hydrogen reducibility of NiO/YSZ composites prepared by impregnation (I), Pechini (P) and physical mixture (PM) procedures.
APA, Harvard, Vancouver, ISO, and other styles
10

Rodríguez-Castillo, José Alberto, Aglaia Ntokou, Ann Atzberger, Michelle D. Tallquist, Werner Seeger, Rory E. Morty, and Katrin Ahlbrecht. "Characterization of pulmonary TCF21 positive cells during neo-alveolarisation." In ERS International Congress 2017 abstracts. European Respiratory Society, 2017. http://dx.doi.org/10.1183/1393003.congress-2017.pa2091.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Reports on the topic "NSO cells"

1

Ehrlich, Marcelo, John S. Parker, and Terence S. Dermody. Development of a Plasmid-Based Reverse Genetics System for the Bluetongue and Epizootic Hemorrhagic Disease Viruses to Allow a Comparative Characterization of the Function of the NS3 Viroporin in Viral Egress. United States Department of Agriculture, September 2013. http://dx.doi.org/10.32747/2013.7699840.bard.

Full text
Abstract:
Project Title: "Development of a plasmid-based reverse genetics system for the Bluetongue and Epizootic Hemorrhagic Disease viruses to allow comparative characterization of the function of the NS3 viroporin in viral egress". Project details: No - IS-4192-09; Participants – Ehrlich M. (Tel Aviv University), Parker J.S. (Cornell University), DermodyT.S. (Vanderbilt University); Period - 2009-2013. Orbiviruses are insect-borne infectious agents of ruminants that cause diseases with considerable economical impact in Israel and the United States. The recent outbreaks of BTV in Europe and of Epizootic Hemorrhagic Disease Virus (EHDV) in Israel, underscore the need for: (i) a better comprehension of the infection process of orbiviruses, (ii) the identification of unique vs. common traits among different orbiviruses, (iii) the development of novel diagnosis and treatment techniques and approaches; all aimed at the achievement of more effective control and treatment measures. It is the context of these broad goals that the present project was carried out. To fulfill our long-term goal of identifying specific viral determinants of virulence, growth, and transmission of the orbiviruses, we proposed to: (i) develop reverse genetics systems for BTV and EHDV2-Ibaraki; and (ii) identify the molecular determinants of the NS3 nonstructural protein related to viroporin/viral egress activities. The first objective was pursued with a two-pronged approach: (i) development of a plasmid-based reverse genetics system for BTV-17, and (ii) development of an "in-vitro" transcription-based reverse genetics system for EHDV2-Ibaraki. Both approaches encountered technical problems that hampered their achievement. However, dissection of the possible causes of the failure to achieve viral spread of EHDV2-Ibaraki, following the transfection of in-vitro transcribed genomic segments of the virus, revealed a novel characteristic of EHDV2-Ibaraki infection: an uncharacteristically low fold increase in titer upon infection of different cell models. To address the function and regulation of NS3 we employed the following approaches: (i) development (together with Anima Cell Metrology) of a novel technique (based on the transfection of fluorescently-labeledtRNAs) that allows for the detection of the levels of synthesis of individual viral proteins (i.e. NS3) in single cells; (ii) development of a siRNA-mediated knockdown approach for the reduction in levels of expression of NS3 in EHDV2-Ibaraki infected cells; (iii) biochemical and microscopy-based analysis of the localization, levels and post-translational modifications of NS3 in infected cells. In addition, we identified the altered regulation and spatial compartmentalization of protein synthesis in cells infected with EHDV2-Ibaraki or the mammalian reovirus. In EHDV2-Ibaraki-infected cells such altered regulation in protein synthesis occurs in the context of a cell stress reponse that includes the induction of apoptosis, autophagy and activation of the stressrelated kinase c-Jun N-terminal Kinase (JNK). Interestingly, inhibition of such stress-related cellular processes diminishes the production of infectious virions, suggesting that EHDV usurps these responses for the benefit of efficient infection. Taken together, while the present project fell short of the generation of novel reverse genetics systems for orbiviruses, the development of novel experimental approaches and techniques, and their employment in the analysis of EHDV-infected cells, yielded novel insights in the interactions of orbiviruses with mammalian cells.
APA, Harvard, Vancouver, ISO, and other styles
2

Meidan, Rina, and Robert Milvae. Regulation of Bovine Corpus Luteum Function. United States Department of Agriculture, March 1995. http://dx.doi.org/10.32747/1995.7604935.bard.

Full text
Abstract:
The main goal of this research plan was to elucidate regulatory mechanisms controlling the development, function of the bovine corpus luteum (CL). The CL contains two different sterodigenic cell types and therefore it was necessary to obtain pure cell population. A system was developed in which granulosa and theca interna cells, isolated from a preovulatory follicle, acquired characteristics typical of large (LL) and small (SL) luteal cells, respectively, as judged by several biochemical and morphological criteria. Experiments were conducted to determine the effects of granulosa cells removal on subsequent CL function, the results obtained support the concept that granulosa cells make a substaintial contribution to the output of progesterone by the cyclic CL but may have a limited role in determining the functional lifespan of the CL. This experimental model was also used to better understand the contribution of follicular granulosa cells to subsequent luteal SCC mRNA expression. The mitochondrial cytochrome side-chain cleavage enzyme (SCC), which converts cholesterol to pregnenolone, is the first and rate-limiting enzyme of the steroidogenic pathway. Experiments were conducted to characterize the gene expression of P450scc in bovine CL. Levels of P450scc mRNA were higher during mid-luteal phase than in either the early or late luteal phases. PGF 2a injection decreased luteal P450scc mRNA in a time-dependent manner; levels were significantly reduced by 2h after treatment. CLs obtained from heifers on day 8 of the estrous cycle which had granulosa cells removed had a 45% reduction in the levels of mRNA for SCC enzymes as well as a 78% reduction in the numbers of LL cells. To characterize SCC expression in each steroidogenic cell type we utilized pure cell populations. Upon luteinization, LL expressed 2-3 fold higher amounts of both SCC enzymes mRNAs than SL. Moreover, eight days after stimulant removal, LL retained their P4 production capacity, expressed P450scc mRNA and contained this protein. In our attempts to establish the in vitro luteinization model, we had to select the prevulatory and pre-gonadotropin surge follicles. The ratio of estradiol:P4 which is often used was unreliable since P4 levels are high in atretic follicles and also in preovulatory post-gonadotropin follicles. We have therefore examined whether oxytocin (OT) levels in follicular fluids could enhance our ability to correctly and easily define follicular status. Based on E2 and OT concentrations in follicular fluids we could more accurately identify follicles that are preovulatory and post gonadotropin surge. Next we studied OT biosynthesis in granulosa cells, cells which were incubated with forskolin contained stores of the precursor indicating that forskolin (which mimics gonadotropin action) is an effective stimulator of OT biosynthesis and release. While studying in vitro luteinization, we noticed that IGF-I induced effects were not identical to those induced by insulin despite the fact that megadoses of insulin were used. This was the first indication that the cells may secrete IGF binding protein(s) which regonize IGFs and not insulin. In a detailed study involving several techniques, we characterized the species of IGF binding proteins secreted by luteal cells. The effects of exogenous polyunsaturated fatty acids and arachidonic acid on the production of P4 and prostanoids by dispersed bovine luteal cells was examined. The addition of eicosapentaenoic acid and arachidonic acid resulted in a dose-dependent reduction in basal and LH-stimulated biosynthesis of P4 and PGI2 and an increase in production of PGF 2a and 5-HETE production. Indomethacin, an inhibitor of arachidonic acid metabolism via the production of 5-HETE was unaffected. Results of these experiments suggest that the inhibitory effect of arachidonic acid on the biosynthesis of luteal P4 is due to either a direct action of arachidonic acid, or its conversion to 5-HETE via the lipoxgenase pathway of metabolism. The detailed and important information gained by the two labs elucidated the mode of action of factors crucially important to the function of the bovine CL. The data indicate that follicular granulosa cells make a major contribution to numbers of large luteal cells, OT and basal P4 production, as well as the content of cytochrome P450 scc. Granulosa-derived large luteal cells have distinct features: when luteinized, the cell no longer possesses LH receptors, its cAMP response is diminished yet P4 synthesis is sustained. This may imply that maintenance of P4 (even in the absence of a Luteotropic signal) during critical periods such as pregnancy recognition, is dependent on the proper luteinization and function of the large luteal cell.
APA, Harvard, Vancouver, ISO, and other styles
3

Delmer, Deborah P., and Prem S. Chourey. The Importance of the Enzyme Sucrose Synthase for Cell Wall Synthesis in Plants. United States Department of Agriculture, October 1994. http://dx.doi.org/10.32747/1994.7568771.bard.

Full text
Abstract:
The goal of this work was to understand the role of the enzyme sucrose synthase (SuSy) in synthesis of cellulose and callose in plants. The work resulting from the this grant leads to a number of conclusions. SuSy clearly plays diverse roles in carbon metabolism. It can associate with the plasma membrane of cells undergoing rapid cellulose deposition, such as cotton fibers, developing maize endosperm, gravistimulated pulvini, and transfer cells of the cotton seed. It is also concentrated at sites of high callose deposition (tapetal cells; cell plates). When SuSy levels are lowered by mutation or by anti-sense technology, cell walls undergo degeneration (maize endosperm) and show reduced levels of cellulose (potato tubers). In sum, our evidence has very much strengthened the concept that SuSy does function in the plasma membrane to channel carbon from sucrose via UDP-glucose to glucan synthase complexes. Soluble SuSy also clearly plays a role in providing carbon for starch synthesis and respiration. Surprisingly, we found that the cotton seed is one unique case where SuSy apparently does not play a role in starch synthesis. Current evidence in sum suggests that no specific SuSy gene encodes the membrane-associated form, although in maize the SS 1 form of SuSy may be most important for cell wall synthesis in the early stages of endosperm development. Work is still in progress to determine what does control membrane localization - and the current evidence we have favors a role for Ca2+, and possibly also protein phosphorylation by differentially regulated protein kinases. Finally, we have discovered for the first time, a major new family of genes that encode the catalytic subunit of the cellulose synthase of plants - a result that has been widely cited and opens many new approaches for the study of this important plant function.
APA, Harvard, Vancouver, ISO, and other styles
4

Casey, Therese, Sameer J. Mabjeesh, Avi Shamay, and Karen Plaut. Photoperiod effects on milk production in goats: Are they mediated by the molecular clock in the mammary gland? United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598164.bard.

Full text
Abstract:
US scientists, Dr. Theresa Casey and Dr. Karen Plaut, collaborated with Israeli scientists, Dr. SameerMabjeesh and Dr. AviShamay to conduct studies proposed in the BARD Project No. US-4715-14 Photoperiod effects on milk production in goats: Are they mediated by the molecular clock in the mammary gland over the last 3 years. CLOCK and BMAL1 are core components of the circadian clock and as heterodimers function as a transcription factor to drive circadian-rhythms of gene expression. Studies of CLOCK-mutant mice found impaired mammary development in late pregnancy was related to poor lactation performance post-partum. To gain a better understanding of role of clock in regulation of mammary development studies were conducted with the mammary epithelial cell line HC11. Decreasing CLOCK protein levels using shRNA resulted in increased mammary epithelial cell growth rate and impaired differentiation, with lower expression of differentiation markers including ad herens junction protein and fatty acid synthesis genes. When BMAL1 was knocked out using CRISPR-CAS mammary epithelial cells had greater growth rate, but reached stationary phase at a lower density, with FACS indicating cells were growing and dying at a faster rate. Beta-casein milk protein levels were significantly decreased in BMAL1 knockout cells. ChIP-seq analysis was conducted to identify BMAL1 target genes in mammary epithelial cells. Studies conducted in goats found that photoperiod duration and physiological state affected the dynamics of the mammary clock. Effects were likely independent of the photoperiod effects on prolactin levels. Interestingly, circadian rhythms of core body temperature, which functions as a key synchronizing cue sent out by the central clock in the hypothalamus, were profoundly affected by photoperiod and physiological state. Data support that the clock in the mammary gland regulates genes important to development of the gland and milk synthesis. We also found the clock in the mammary is responsive to changes in physiological state and photoperiod, and thus may serve as a mechanism to establish milk production levels in response to environmental cues.
APA, Harvard, Vancouver, ISO, and other styles
5

Semaan, Dima, and Linda Scobie. Feasibility study for in vitro analysis of infectious foodborne HEV. Food Standards Agency, September 2022. http://dx.doi.org/10.46756/sci.fsa.wfa626.

Full text
Abstract:
Hepatitis E virus (HEV) is a member of the Hepeviridae family capable of infecting humans producing a range of symptoms from mild disease to kidney failure. Epidemiological evidence suggests that hepatitis E genotype III and IV cases may be associated with the consumption of undercooked pork meat, offal and processed products such as sausages [1]. A study carried out by the Animal Health and Veterinary Laboratories Agency (AHVLA), found hepatitis E virus contamination in the UK pork production chain and that 10% of a small sample of retail pork sausages were contaminated with the virus [2]. Furthermore, studies have confirmed the presence of HEV in the food chain and the foodborne transmission of Hepatitis E virus to humans [reviewed in 5]. Likewise, Scottish shellfish at retail [6] have also been found positive for HEV viral nucleic acid and some preliminary studies indicate that the virus is also detectable in soft fruits (L Scobie; unpublished data). There are current misunderstandings in what this data represents, and these studies have raised further questions concerning the infectivity of the virus, the processing of these foods by industry and the cooking and/or preparation by caterers and consumers. There are significant gaps in the knowledge around viral infectivity, in particular the nature of the preparation of food matrices to isolate the virus, and also with respect to a consistent and suitable assay for confirming infectivity [1,3]. Currently, there is no suitable test for infectivity, and, in addition, we have no knowledge if specific food items would be detrimental to cells when assessing the presence of infectious virus in vitro. The FSA finalised a comprehensive critical review on the approaches to assess the infectivity of the HEV virus which is published [3] recommending that a cell culture based method should be developed for use with food. In order to proceed with the development of an infectivity culture method, there is a requirement to assess if food matrices are detrimental to cell culture cell survival. Other issues that may have affected the ability to develop a consistent method are the length of time the virally contaminated sample is exposed to the cells and the concentration of the virus present. In most cases, the sample is only exposed to the cells for around 1 hour and it has been shown that if the concentration is less that 1x103 copies then infection is not established [3,5,10,11].
APA, Harvard, Vancouver, ISO, and other styles
6

Pell, Eva J., Sarah M. Assmann, Amnon Schwartz, and Hava Steinberger. Ozone Altered Stomatal/Guard Cell Function: Whole Plant and Single Cell Analysis. United States Department of Agriculture, December 2000. http://dx.doi.org/10.32747/2000.7573082.bard.

Full text
Abstract:
Original objectives (revisions from original proposal are highlighted) 1. Elucidate the direct effects O3 and H2O2 on guard cell function, utilizing assays of stomatal response in isolated epidermal peels and whole cell gas exchange. 2. Determine the mechanistic basis of O3 and H2O2 effects on the plasma membrane through application of the electrophysiological technique of patch clamping to isolated guard cells. 3. Determine the relative sensitivity of Israeli cultivars of economically important crops to O3 and determine whether differential leaf conductance responses to O3 can explain relative sensitivity to the air pollutant: transfer of technological expertise to Israel. Background to the topic For a long time O3 has been known to reduce gas exchange in plants; it has however been unclear if O3 can affect the stomatal complex directly. Ion channels are essential in stomatal regulation, but O3 has never before been shown to affect these directly. Major conclusions, solution, achievements 1. Ozone inhibits light-induced stomatal opening in epidermal peels isolated from Vicia faba, Arabidopsis thaliana and Nicotiana tabacum in V. faba plants this leads to reduced assimilation without a direct effect on the photosynthetic apparatus. Stomatal opening is more sensitive to O3 than stomatal closure. 2. Ozone causes inhibition of inward K+ channels (involved in stomatal opening) while no detectable effect is observed o the outward K+ channels (stomatal closure). 3. Hydrogen peroxide inhibits stomatal opening and induces stomatal closure in epidermal peels isolated from Vicia faba. 4. Hydrogen peroxide enhances stomatal closure by increasing K+ efflux from guard cells via outward rectifying K+ channels. 5. Based on epidermal peel experiments we have indirectly shown that Ca2+ may play a role in the guard cell response to O3. However, direct measurement of the guard cell [Ca2+]cyt did not show a response to O3. 6. Three Israeli cultivars of zucchini, Clarita, Yarden and Bareqet, were shown to be relatively sensitive to O3 (0.12 ml1-1 ). 7. Two environmentally important Israeli pine species are adversely affected by O3, even at 0.050 ml1-1 , a level frequently exceeded under local tropospheric conditions. P. brutia may be better equipped than P. halepensis to tolerate O3 stress. 8. Ozone directly affects pigment biosynthesis in pine seedlings, as well as the metabolism of O5 precursors, thus affecting the allocation of resources among various metabolic pathways. 9. Ozone induces activity of antioxidant enzymes, and of ascorbate content i the mesophyll and epidermis cells of Commelina communis L. Implications, both scientific and agricultural We have improved the understanding of how O3 and H2O2 do affect guard cell and stomatal function. We have shown that economical important Israeli species like zucchini and pine are relatively sensitive to O3.
APA, Harvard, Vancouver, ISO, and other styles
7

de Sousa, Eduardo, Renata Matsui, Leonardo Boldrini, Leandra Baptista, and José Mauro Granjeiro. Mesenchymal stem cells for the treatment of articular cartilage defects of the knee: an overview of systematic reviews. INPLASY - International Platform of Registered Systematic Review and Meta-analysis Protocols, December 2022. http://dx.doi.org/10.37766/inplasy2022.12.0114.

Full text
Abstract:
Review question / Objective: Population: adults (aged between 18 and 50 years) with traumatic knee lesions who underwent treatment with mesenchymal stem cells; Intervention: defined by the treatment with mesenchymal stem cells; The comparison group: treatment with autologous chondrocytes or microfracture treatments; Primary outcome: formation of cartilage neo tissue in the defect area, determined by magnetic resonance imaging (MRI) or by direct visualization in second-look knee arthroscopy.; Secondary outcomes: based on clinical scores such as visual analog scale (VAS) for pain, Western Ontario and McMaster universities score (WOMAC), knee society score (KSS), Tegner and Lysholm.
APA, Harvard, Vancouver, ISO, and other styles
8

Toney, Mike. Develop and Test TXM Battery Cells CRADA No.: 14-044C. Office of Scientific and Technical Information (OSTI), April 2014. http://dx.doi.org/10.2172/1542607.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Carpita, Nicholas C., Ruth Ben-Arie, and Amnon Lers. Pectin Cross-Linking Dynamics and Wall Softening during Fruit Ripening. United States Department of Agriculture, July 2002. http://dx.doi.org/10.32747/2002.7585197.bard.

Full text
Abstract:
Our study was designed to elucidate the chemical determinants of pectin cross-linking in developing fruits of apple and peach and to evaluate the role of breakage cross-linkages in swelling, softening, and cell separation during the ripening. Peaches cell walls soften and swell considerably during the ripening, whereas apples fruit cells maintain wall firmness but cells separate during late stages of ripening. We used a "double-reduction" technique to show that levels of non-methyl esters of polyuronic acid molecules were constant during the development and ripening and decreased only in overripe fruit. In peach, methyl and non-methyl esters increased during the development and decreased markedly during the ripening. Non-methyl ester linkages in both fruit decreased accompanied fruit softening. The identity of the second component of the linkage and its definitive role in the fruit softening remain elusive. In preliminary examination of isolated apples cell walls, we found that phenolic compounds accumulate early in wall development but decrease markedly during ripening. Quantitative texture analysis was used to correlate with changes to wall chemistry from the fresh-picked ripe stage to the stage during storage when the cell separation occurs. Cell wall composition is similar in all cultivars, with arabinose as the principal neutral sugar. Extensive de-branching of these highly branched arabinans pre-stages softening and cell-cell separation during over-ripening of apple. The longer 5-arabinans remain attached to the major pectic polymer rhamnogalacturonan I (RG I) backbone. The degree of RG I branching, as judged from the ratios of 2-Rha:2,4-Rha, also decreases, specially after an extensive arabinan de-branching. Loss of the 4-Rham linkages correlated strongly with the softening of the fruit. Loss of the monomer or polymer linked to the RG I produce directly or indirectly the softening of the fruit. This result will help to understand the fruit softening and to have better control of the textural changes in fruit during the ripening and especially during the storage. 'Wooliness', an undesirable mealy texture that is induced during chilling of some peach cultivars, greatly reduces the fruit storage possibilities. In order to examine the hypothesis that the basis for this disorder is related to abnormality in the cell wall softening process we have carried out a comparative analysis using the resistant cultivar, Sunsnow, and a sensitive one, Hermosa. We investigated the activity of several pectin- and glycan-modifying enzymes and the expression of their genes during ripening, chilling, and subsequent shelf-life. The changes in carbohydrate status and in methyl vs. non-methyl uronate ester levels in the walls of these cultivars were examined as well to provide a basis for comparison of the relevant gene expression that may impact appearance of the wooly character. The activities of the specific polygalacturonase (PGase) and a CMC-cellulase activities are significantly elevated in walls of peaches that have become wooly. Cellulase activities correlated well with increased level of the transcript, but differential expression of PGase did not correspond with the observed pattern of mRNA accumulation. When expression of ethylene biosynthesis related genes was followed no significant differences in ACC synthase gene expression was observed in the wooly fruit while the normal activation of the ACC oxidase was partially repressed in the Hermosa wooly fruits. Normal ripening-related loss of the uronic acid-rich polymers was stalled in the wooly Hermosa inconsistent with the observed elevation in a specific PGase activity but consistent with PG gene expression. In general, analysis of the level of total esterification, degree of methyl esterification and level of non-methyl esters did not reveal any major alterations between the different fruit varieties or between normal and abnormal ripening. Some decrease in the level of uronic acids methyl esterification was observed for both Hermosa and Sunsnow undergoing ripening following storage at low temperature but not in fruits ripening after harvest. Our results support a role for imbalanced cell wall degradation as a basis for the chilling disorder. While these results do not support a role for the imbalance between PG and pectin methyl esterase (PME) activities as the basis for the disorder they suggest a possible role for imbalance between cellulose and other cell wall polymer degradation during the softening process.
APA, Harvard, Vancouver, ISO, and other styles
10

Nam, Suk Woo, Hyung-Joon Choi, and Tae Hoon Lim. Modeling and simulation of NiO dissolution and Ni deposition in molten carbonate fuel cells. Office of Scientific and Technical Information (OSTI), December 1996. http://dx.doi.org/10.2172/460244.

Full text
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'NSO cells' for particular source types:
Journal articles Dissertations / Theses Books
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography