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Journal articles on the topic 'Non-STEC Escherichia coli'

Author: Grafiati

Published: 25 November 2024

Last updated: 31 July 2025

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1

MATHUSA, EMILY C., YUHUAN CHEN, ELENA ENACHE, and LLOYD HONTZ. "Non-O157 Shiga Toxin–Producing Escherichia coli in Foods." Journal of Food Protection 73, no. 9 (2010): 1721–36. http://dx.doi.org/10.4315/0362-028x-73.9.1721.

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Non-O157 Shiga toxin–producing Escherichia coli (STEC) strains have been linked to outbreaks and sporadic cases of illness worldwide. Illnesses linked to STEC serotypes other than O157:H7 appear to be on the rise in the United States and worldwide, indicating that some of these organisms may be emerging pathogens. As more laboratories are testing for these organisms in clinical samples, more cases are uncovered. Some cases of non-O157 STEC illness appear to be as severe as cases associated with O157, although in general cases attributed to non-O157 are less severe. There is much variation in v

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2

Paquette, Sarah-Jo, Rahat Zaheer, Kim Stanford, James Thomas, and Tim Reuter. "Competition among Escherichia coli Strains for Space and Resources." Veterinary Sciences 5, no. 4 (2018): 93. http://dx.doi.org/10.3390/vetsci5040093.

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Shiga toxin-producing Escherichia coli (STEC) are a subgroup of E. coli causing human diseases. Methods to control STEC in livestock and humans are limited. These and other emerging pathogens are a global concern and novel mitigation strategies are required. Habitats populated by bacteria are subjected to competition pressures due to limited space and resources but they use various strategies to compete in natural environments. Our objective was to evaluate non-pathogenic E. coli strains isolated from cattle feces for their ability to out-compete STEC. Competitive fitness of non-pathogenic E.

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3

SMITH, JAMES L., and PINA M. FRATAMICO. "Effect of Stress on Non-O157 Shiga Toxin–Producing Escherichia coli†." Journal of Food Protection 75, no. 12 (2012): 2241–50. http://dx.doi.org/10.4315/0362-028x.jfp-12-255.

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Non-O157 Shiga toxin–producing Escherichia coli (non-O157 STEC) strains have emerged as important foodborne pathogens worldwide. Non-O157 STEC serogroups O26, O45, O103, O111, O121, and O145 have been declared as adulterants in beef by the U.S. Department of Agriculture Food Safety and Inspection Service. While documentation is limited, treatments including heat and acid that have been shown to inactivate E. coli O157:H7 will likely also destroy non-O157 STEC; however, non-O157 STEC strains show variability in their responses to stress. It has been shown that non-O157 STEC may survive in ferme

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4

CERNICCHIARO, N., D. G. RENTER, C. A. CULL, Z. D. PADDOCK, X. SHI, and T. G. NAGARAJA. "Fecal Shedding of Non-O157 Serogroups of Shiga Toxin–Producing Escherichia coli in Feedlot Cattle Vaccinated with an Escherichia coli O157:H7 SRP Vaccine or Fed a Lactobacillus-Based Direct-Fed Microbial†." Journal of Food Protection 77, no. 5 (2014): 732–37. http://dx.doi.org/10.4315/0362-028x.jfp-13-358.

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The objectives of this study were to determine whether fecal shedding of non-O157 Shiga toxin–producing Escherichia coli (STEC) in feedlot cattle was affected by the use of an E. coli O157:H7 vaccine or a direct-fed microbial (DFM) and whether the shedding of a particular non-O157 STEC serogroup within feces was associated with shedding of O157 or other non-O157 STEC serogroups. A total of 17,148 cattle in 40 pens were randomized to receive one, both, or neither (control) of the two interventions: a vaccine based on the siderophore receptor and porin proteins (E. coli SRP vaccine, two doses) a

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5

HUSSEIN, HUSSEIN S., and LAURIE M. BOLLINGER. "Prevalence of Shiga Toxin–Producing Escherichia coli in Beef Cattle." Journal of Food Protection 68, no. 10 (2005): 2224–41. http://dx.doi.org/10.4315/0362-028x-68.10.2224.

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A large number of Shiga toxin–producing Escherichia coli (STEC) strains have caused major outbreaks and sporadic cases of human illnesses, including mild diarrhea, bloody diarrhea, hemorrhagic colitis, and the life-threatening hemolytic uremic syndrome. These illnesses have been traced to both O157 and non-O157 STEC. In a large number of STEC-associated outbreaks, the infections were attributed to consumption of ground beef or other beef products contaminated with cattle feces. Thus, beef cattle are considered reservoirs of STEC and can pose significant health risks to humans. The global natur

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6

KARCH, HELGE, HANS-IKO HUPPERTZ, JOCHEN BOCKEMÜHL, HERBERT SCHMIDT, ANDREAS SCHWARZKOPF, and REINHARD LISSNER. "Shiga Toxin-Producing Escherichia coli Infections in Germany†." Journal of Food Protection 60, no. 11 (1997): 1454–57. http://dx.doi.org/10.4315/0362-028x-60.11.1454.

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A prospective study was carried out in collaboration with two children's hospitals in Würzburg, Germany to assess the incidence and clinical manifestations of infections due to Shiga toxin-producing Escherichia coli (STEC) in children. Between 1991 and 1995, stool samples from 2788 children with enteritis were investigated for the occurrence of STEC. STEC cultures from stools were screened using PCR with primers complementary to Shiga toxin 1(Stx1) and Shiga toxin 2 (Stx2) genes. PCR-positive samples were further subjected to colony blot hybridization and probe positive colonies were serotyped

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7

ACHESON, DAVID W. K. "How Does Escherichia coli O157:H7 Testing in Meat Compare with What We Are Seeing Clinically?" Journal of Food Protection 63, no. 6 (2000): 819–21. http://dx.doi.org/10.4315/0362-028x-63.6.819.

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Escherichia coli O157:H7 is but one of a group of Shiga toxin-producing E. coli (STEC) that cause both intestinal disease such as bloody and nonbloody diarrhea and serious complications like hemolytic uremic syndrome (HUS). While E. coli O157: H7 is the most renowned STEC, over 200 different types of STEC have been documented in meat and animals, at least 60 of which have been linked with human disease. A number of studies have suggested that non-O157 STEC are associated with clinical disease, and non-O157 STEC are present in the food supply. Non-O157 STEC, such as O111 have caused large outbr

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8

TERAO, YOSHITAKA, KANA TAKESHITA, YASUTAKA NISHIYAMA, NAOKI MORISHITA, TAKASHI MATSUMOTO, and FUMIKI MORIMATSU. "Promising Nucleic Acid Lateral Flow Assay Plus PCR for Shiga Toxin–Producing Escherichia coli." Journal of Food Protection 78, no. 8 (2015): 1560–68. http://dx.doi.org/10.4315/0362-028x.jfp-14-495.

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Shiga toxin (Stx)–producing Escherichia coli (STEC) is a frequent cause of foodborne infections, and methods for rapid and reliable detection of STEC are needed. A nucleic acid lateral flow assay (NALFA) plus PCR was evaluated for detecting STEC after enrichment. When cell suspensions of 45 STEC strains, 14 non-STEC strains, and 13 non–E. coli strains were tested with the NALFA plus PCR, all of the STEC strains yielded positive results, and all of the non-STEC and non–E. coli strains yielded negative results. The lower detection limit for the STEC strains ranged from 0.1 to 1 pg of genomic DNA

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9

ENACHE, ELENA, EMILY C. MATHUSA, PHILIP H. ELLIOTT, et al. "Thermal Resistance Parameters for Shiga Toxin–Producing Escherichia coli in Apple Juice." Journal of Food Protection 74, no. 8 (2011): 1231–37. http://dx.doi.org/10.4315/0362-028x.jfp-10-488.

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The purpose of the present study was to determine the heat resistance of six non-O157 Shiga toxin–producing Escherichia coli (STEC) serotypes in comparison to E. coli O157:H7 in single-strength apple juice without pulp. The thermal parameters for stationary-phase and acid-adapted cells of E. coli strains from serogroups O26, O45, O103, O111, O121, O145, and O157:H7 were determined by using an immersed coil apparatus. The most heat-sensitive serotype in the present study was O26. Stationary-phase cells for serotypes O145, O121, and O45 had the highest D56°C-value among the six non-O157 serotype

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10

THRAN, B. H., H. S. HUSSEIN, M. R. HALL, and S. F. KHAIBOULLINA. "Shiga Toxin–Producing Escherichia coli in Beef Heifers Grazing an Irrigated Pasture." Journal of Food Protection 64, no. 10 (2001): 1613–16. http://dx.doi.org/10.4315/0362-028x-64.10.1613.

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Shiga toxin–producing Escherichia coli (STEC) produce toxins that have been associated with several human illnesses. E. coli O157:H7 is the most well-studied STEC and was first associated with consumption of improperly cooked ground beef in 1982. E. coli O157:H7 is not the only foodborne STEC because other STEC serotypes are also associated with human illnesses. The objective of this study was to assess prevalence of STEC in 23 yearling beef (Angus) heifers grazing an irrigated grass pasture in spring (April), summer (July), fall (October), and winter (December) of 1999. A total of 86 fecal sa

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11

KATAOKA, AI, ELENA ENACHE, MARIA SOHAIL, PHILIP H. ELLIOTT, and D. GLENN BLACK. "Inactivation of Shiga Toxin–Producing Escherichia coli in Single-Strength Lemon and Lime Juices Containing Preservatives." Journal of Food Protection 74, no. 10 (2011): 1746–50. http://dx.doi.org/10.4315/0362-028x.jfp-11-083.

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The objective of this study was to determine the inactivation of non-O157 Shiga toxin–producing Escherichia coli (STEC) serotypes in comparison with O157 STEC in commercially produced, shelf-stable lemon and lime juices. The present validation tests confirmed that storage of the juices containing preservatives at room temperatures (22°C) for 3 days (72 h) ensures a &gt;6-log reduction of O26, O45, O103, O111, O121, O145, and O157 STEC. These results demonstrate that non-O157 STEC had survival abilities comparable to those of E. coli O157:H7 strains in acidic food products such as lemon and

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12

Tack, Danielle M., Hannah M. Kisselburgh, LaTonia C. Richardson, et al. "Shiga Toxin-Producing Escherichia coli Outbreaks in the United States, 2010–2017." Microorganisms 9, no. 7 (2021): 1529. http://dx.doi.org/10.3390/microorganisms9071529.

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Shiga toxin-producing Escherichia coli (STEC) cause illnesses ranging from mild diarrhea to ischemic colitis and hemolytic uremic syndrome (HUS); serogroup O157 is the most common cause. We describe the epidemiology and transmission routes for U.S. STEC outbreaks during 2010–2017. Health departments reported 466 STEC outbreaks affecting 4769 persons; 459 outbreaks had a serogroup identified (330 O157, 124 non-O157, 5 both). Among these, 361 (77%) had a known transmission route: 200 foodborne (44% of O157 outbreaks, 41% of non-O157 outbreaks), 87 person-to-person (16%, 24%), 49 animal contact (

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13

TAYLOR, E. V., T. A. NGUYEN, K. D. MACHESKY, et al. "Multistate Outbreak of Escherichia coli O145 Infections Associated with Romaine Lettuce Consumption, 2010‡§." Journal of Food Protection 76, no. 6 (2013): 939–44. http://dx.doi.org/10.4315/0362-028x.jfp-12-503.

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Non-O157 Shiga toxin–producing Escherichia coli (STEC) can cause severe illness, including hemolytic uremic syndrome (HUS). STEC O145 is the sixth most commonly reported non-O157 STEC in the United States, although outbreaks have been infrequent. In April and May 2010, we investigated a multistate outbreak of STEC O145 infection. Confirmed cases were STEC O145 infections with isolate pulsed-field gel electrophoresis patterns indistinguishable from those of the outbreak strain. Probable cases were STEC O145 infections or HUS in persons who were epidemiologically linked. Case-control studies wer

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14

Kudva, Indira T., Erika N. Biernbaum, and Julian M. Trachsel. "Growth dynamics and protein-expression of Escherichia coli serotypes O26:H11, O111:H8 and O145:NM in the bovine rumen." PLOS One 20, no. 6 (2025): e0313978. https://doi.org/10.1371/journal.pone.0313978.

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To adapt to the ruminal environment, Shiga toxin-producing Escherichia coli (STEC) O157:H7 (O157) expresses proteins involved in survival rather than virulence. Additionally, STEC O157 strains exhibit distinct in vitro but shared in vivo survival patterns in rumen fluids that sets them apart from non-STEC, commensal E. coli. To determine if similar responses would be observed with other STEC, we evaluated three non-O157 serotypes, O26:H11, O111:H8 and O145:NM, along with a non-STEC E. coli, under the growth conditions used for STEC O157: (i) anaerobically, in vitro, in rumen fluid from cattle

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15

LITT, PUSHPINDER KAUR, JOYJIT SAHA, and DIVYA JARONI. "Characterization of Bacteriophages Targeting Non-O157 Shiga Toxigenic Escherichia coli." Journal of Food Protection 81, no. 5 (2018): 785–94. http://dx.doi.org/10.4315/0362-028x.jfp-17-460.

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ABSTRACT Non-O157 Shiga toxigenic Escherichia coli (STEC) are an important group of foodborne pathogens, implicated in several outbreaks and recalls in the past 2 decades. It is therefore crucial to devise effective control strategies against these pathogens. Bacteriophages present an attractive alternative to conventional pathogen control methods in the food industry. Bacteriophages, targeting non-O157 STEC (O26, O45, O103, O111, O121, O145), were isolated from beef cattle operations in Oklahoma. Their host range and lytic ability were determined against several (n = 21) non-O157 STEC isolate

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16

Szczerba-Turek, Anna, Filomena Chierchia, Piotr Socha, and Wojciech Szweda. "Shiga Toxin-Producing Escherichia coli in Faecal Samples from Wild Ruminants." Animals 13, no. 5 (2023): 901. http://dx.doi.org/10.3390/ani13050901.

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Wildlife can harbour Shiga toxin-producing Escherichia coli (STEC). In the present study, STEC in faecal samples from red deer (n = 106) and roe deer (n = 95) were characterised. All isolates were non-O157 strains. In red deer, STEC were detected in 17.9% (n = 19) of the isolates, and the eae/stx2b virulence profile was detected in two isolates (10.5%). One STEC strain harboured stx1a (5.3%) and eighteen STEC strains harboured stx2 (94.7%). The most prevalent stx2 subtypes were stx2b (n = 12; 66.7%), stx2a (n = 3; 16.7%), and stx2g (n = 2; 11.1%). One isolate could not be subtyped (NS) with th

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17

Cloke, Jonathan, Sharon Matheny, Michelle Swimley, et al. "Validation of the Applied Biosystems RapidFinder Shiga Toxin–Producing E. coli (STEC) Detection Workflow." Journal of AOAC INTERNATIONAL 99, no. 6 (2016): 1537–54. http://dx.doi.org/10.5740/jaoacint.16-0235.

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Abstract The Applied Biosystems™ RapidFinder™ STEC Detection Workflow (Thermo Fisher Scientific) is a complete protocol for the rapid qualitative detection of Escherichia coli (E. coli) O157:H7 and the “Big 6” non-O157 Shiga-like toxin-producing E. coli (STEC) serotypes (defined as serogroups: O26, O45, O103, O111, O121, and O145). The RapidFinder STEC Detection Workflow makes use of either the automated preparation of PCR-ready DNA using the Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit in conjunction with the Applied Biosystems MagMAX™ Express 96-well magnetic particle processor or

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18

Ferdous, Mithila, Kai Zhou, Alexander Mellmann, et al. "Is Shiga Toxin-Negative Escherichia coli O157:H7 Enteropathogenic or Enterohemorrhagic Escherichia coli? Comprehensive Molecular Analysis Using Whole-Genome Sequencing." Journal of Clinical Microbiology 53, no. 11 (2015): 3530–38. http://dx.doi.org/10.1128/jcm.01899-15.

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The ability ofEscherichia coliO157:H7 to induce cellular damage leading to disease in humans is related to numerous virulence factors, most notably thestxgene, encoding Shiga toxin (Stx) and carried by a bacteriophage. Loss of the Stx-encoding bacteriophage may occur during infection or culturing of the strain. Here, we collectedstx-positive andstx-negative variants ofE. coliO157:H7/NM (nonmotile) isolates from patients with gastrointestinal complaints. Isolates were characterized by whole-genome sequencing (WGS), and their virulence properties and phylogenetic relationship were determined. Be

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Novotna, R., P. Alexa, J. Hamrik, A. Madanat, J. Smola, and A. Cizek. "Isolation and characterization Shiga toxin-producing Escherichia coli from sheep and goats inJordanwith evidence of multiresistant serotype O157:H7." Veterinární Medicína 50, No. 3 (2012): 111–18. http://dx.doi.org/10.17221/5603-vetmed.

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Ninety-three rectal swabs of lambs and young goats from two extensively and two intensively managed herds in Jordanwere taken and examined for Shiga toxin-producing Escherichia coli (STEC). The bacteriological examination included the preenrichment of rectal swabs in EC broth with novobiocin, and a subsequent parallel isolation on enterohemolysin agar and immunomagnetic separation with cultivation on CT-SMAC. The STEC O157:H7 strains were demonstrated in 8 of 32 diarrheic lambs 1- to 3-weeks old in one sheep herd with intensive milk production. In the remaining three herds, serogroups O128, O7

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20

Zhang, Yujie, Yen-Te Liao, Xiaohong Sun, and Vivian C. H. Wu. "Is Shiga Toxin-Producing Escherichia coli O45 No Longer a Food Safety Threat? The Danger is Still Out There." Microorganisms 8, no. 5 (2020): 782. http://dx.doi.org/10.3390/microorganisms8050782.

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Many Shiga toxin-producing Escherichia coli (STEC) strains, including the serogroups of O157 and most of the top six non-O157 serotypes, are frequently associated with foodborne outbreaks. Therefore, they have been extensively studied using next-generation sequencing technology. However, related information regarding STEC O45 strains is scarce. In this study, three environmental E. coli O45:H16 strains (RM11911, RM13745, and RM13752) and one clinical E. coli O45:H2 strain (SJ7) were sequenced and used to characterize virulence factors using two reference E. coli O45:H2 strains of clinical orig

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21

Gonçalves, V. P., and J. M. Marin. "Fate of non O157 Shiga toxigenic Escherichia coli in composted cattle manure." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 59, no. 4 (2007): 825–31. http://dx.doi.org/10.1590/s0102-09352007000400001.

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To determine the fate of Shiga toxigenic Escherichia coli (STEC) non-O 157 in composted manure from naturally colonized cattle, fresh manure was obtained from three cows carrying non-O157 STEC strains possessing the stx2 gene. Two composting systems were used: a 0.6m deep cave opened in the soil and an one meter high solid manure heap in a pyramidal architecture. Every day, for the 10 first days, and every five days for a month, one manure sample from three different points in both systems was collected and cultured to determine the presence of E. coli and the presence of the stx 2 gene in the

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22

Wang, Hongye, Muthu Dharmasena, Zhao Chen, and Xiuping Jiang. "Persistence of Non-O157 Shiga Toxin–Producing Escherichia coli in Dairy Compost during Storage." Journal of Food Protection 80, no. 12 (2017): 1999–2005. http://dx.doi.org/10.4315/0362-028x.jfp-16-552.

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ABSTRACT Dairy compost with 20, 30, or 40% moisture content (MC) was inoculated with a mixture of six non-O157 Shiga toxin–producing Escherichia coli (STEC) serovars at a final concentration of 5.1 log CFU/g and then stored at 22 and 4°C for 125 days. Six storage conditions—4°C and 20% MC, 4°C and 30% MC, 4°C and 40% MC, 22°C and 20% MC, 22°C and 30% MC, and 22°C and 40% MC—were investigated for the persistence of non-O157 STEC in the dairy compost. During the entire storage, fluctuations in indigenous mesophilic bacterial levels were observed within the first 28 days of storage. After inocula

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LIVEZEY, KRISTIN W., BETTINA GROSCHEL, and MICHAEL M. BECKER. "Use of the ecf1 Gene To Detect Shiga Toxin–Producing Escherichia coli in Beef Samples." Journal of Food Protection 78, no. 4 (2015): 675–84. http://dx.doi.org/10.4315/0362-028x.jfp-14-417.

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Escherichia coli O157:H7 and six serovars (O26, O103, O121, O111, O145, and O45) are frequently implicated in severe clinical illness worldwide. Standard testing methods using stx, eae, and O serogroup–specific gene sequences for detecting the top six non-O157 STEC bear the disadvantage that these genes may reside, independently, in different nonpathogenic organisms, leading to false-positive results. The ecf operon has previously been identified in the large enterohemolysin-encoding plasmid of eae-positive Shiga toxin–producing E. coli (STEC). Here, we explored the utility of the ecf operon a

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CHATZIKYRIAKIDOU, K., R. R. GEIER, S. C. INGHAM, and B. H. INGHAM. "Growth of Strains of the Major Non-O157 Shiga Toxin–Producing Escherichia coli Serogroups Is Not Different from Growth of Escherichia coli O157:H7 in Neutral Broth (pH 7.4) and Acidified Broth (pH 5.6) at 10°C." Journal of Food Protection 77, no. 9 (2014): 1617–23. http://dx.doi.org/10.4315/0362-028x.jfp-14-048.

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Understanding the survival and growth of non-O157 Shiga toxin–producing Escherichia coli (STEC) strains under cold temperatures may be important for protecting public health. The aim of this study was to compare the growth of three strains of each of the major non-O157 STEC serogroups (O26, O45, O103, O111, O121, and O145) with the growth of six O157:H7 STEC strains in broth at 10°C. Brain heart infusion broth (BHIB; pH 7.4) was inoculated with a single strain of stationary-phase STEC culture to produce a starting inoculum of ~106 CFU/ml and stored at 10°C for up to 96 h (three trials per stra

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MAKINO, S., H. KOBORI, H. ASAKURA, et al. "Detection and characterization of Shiga toxin-producing Escherichia coli from seagulls." Epidemiology and Infection 125, no. 1 (2000): 55–61. http://dx.doi.org/10.1017/s0950268899004100.

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Shiga toxin (Stx)-producing Escherichia coli (STEC) strains isolated from a seagull in Japan were examined. A total of 50 faecal samples was collected on a harbour bank in Hokkaido, Japan, in July 1998. Two different STEC strains, whose serotypes were O136[ratio ]H16 and O153[ratio ]H− , were isolated from the same individual by PCR screening; both of them were confirmed by ELISA and Vero cell cytotoxicity assay to be producing active Stx2 and Stx1, respectively. They harboured large plasmids, but did not carry the haemolysin or eaeA genes of STEC O157[ratio ]H7. Based on their plasmid profile

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Rosser, Tracy, Tracy Dransfield, Lesley Allison, et al. "Pathogenic Potential of Emergent Sorbitol-Fermenting Escherichia coli O157:NM." Infection and Immunity 76, no. 12 (2008): 5598–607. http://dx.doi.org/10.1128/iai.01180-08.

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ABSTRACT Non-sorbitol-fermenting (NSF) Escherichia coli O157:H7 is the primary Shiga toxin-producing E. coli (STEC) serotype associated with human infection. Since 1988, sorbitol-fermenting (SF) STEC O157:NM strains have emerged and have been associated with a higher incidence of progression to hemolytic-uremic syndrome (HUS) than NSF STEC O157:H7. This study investigated bacterial factors that may account for the increased pathogenic potential of SF STEC O157:NM. While no evidence of toxin or toxin expression differences between the two O157 groups was found, the SF STEC O157:NM strains adher

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Dean-Nystrom, Evelyn A., Angela R. Melton-Celsa, Joachim F. L. Pohlenz, Harley W. Moon, and Alison D. O'Brien. "Comparative Pathogenicity of Escherichia coli O157 and Intimin-Negative Non-O157 Shiga Toxin-Producing E. coli Strains in Neonatal Pigs." Infection and Immunity 71, no. 11 (2003): 6526–33. http://dx.doi.org/10.1128/iai.71.11.6526-6533.2003.

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ABSTRACT We compared the pathogenicity of intimin-negative non-O157:H7 Shiga toxin (Stx)-producing Escherichia coli (STEC) O91:H21 and O104:H21 strains with the pathogenicity of intimin-positive O157:H7 and O157:H− strains in neonatal pigs. We also examined the role of Stx2d-activatable genes and the large hemolysin-encoding plasmid of O91:H21 strain B2F1 in the pathogenesis of STEC disease in pigs. We found that all E. coli strains that made wild-type levels of Stx caused systemic illness and histological lesions in the brain and intestinal crypts, whereas none of the control Stx-negative E.

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Skinner, Craig, Guodong Zhang, Stephanie Patfield, and Xiaohua He. "AnIn VitroCombined Antibiotic-Antibody Treatment Eliminates Toxicity from Shiga Toxin-Producing Escherichia coli." Antimicrobial Agents and Chemotherapy 59, no. 9 (2015): 5435–44. http://dx.doi.org/10.1128/aac.00763-15.

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ABSTRACTTreating Shiga toxin-producingEscherichia coli(STEC) gastrointestinal infections is difficult. The utility of antibiotics for STEC treatment is controversial, since antibiotic resistance among STEC isolates is widespread and certain antibiotics dramatically increase the expression of Shiga toxins (Stxs), which are some of the most important virulence factors in STEC. Stxs contribute to life-threatening hemolytic uremic syndrome (HUS), which develops in considerable proportions of patients with STEC infections. Understanding the antibiotic resistance profiles of STEC isolates and the St

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Hosking, Edan, Brooke Roman, Susan Alles, et al. "NeoSeekTM STEC: A Multiplex Molecular Method for Detection and Identification of Select Shiga Toxin–Producing Escherichia coli in Beef." Journal of AOAC INTERNATIONAL 103, no. 2 (2020): 523–32. http://dx.doi.org/10.5740/jaoacint.19-0300.

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Abstract Background: NeoSeekTM STEC is a single-source, service-based method for detection and identification of select Shiga toxin–producing Escherichia coli (STEC), including E. coli O157:H7 and STEC of somatic groups O26, O45, O103, O111, O121, and O145. The method is a multiplex molecular method utilizing more than 80 genetic targets to identify STEC in complex matrices such as food enrichment cultures. Objective: A study was conducted to validate the NeoSeek method for detection of select STEC in raw beef trim. Methods: Performance of the NeoSeek STEC method was compared with that of the

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Faulds, Nikki, Katharine Evans, Jessica Williams, et al. "Validation of the Thermo Scientific SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay and SureTect™ Escherichia coli STEC Identification PCR Assay for the Detection of Escherichia coli O157:H7 and the Escherichia coli STEC Serotypes (O26, O45, O103, O111, O121, O145) from Fresh Raw Spinach, Fresh Baby Leaves, Fresh Cut Tomatoes, Frozen Raw Beef, Raw Beef Trim, and Beef Carcass Sponges: AOAC Performance Tested MethodSM 012102." Journal of AOAC INTERNATIONAL 105, no. 2 (2021): 521–48. http://dx.doi.org/10.1093/jaoacint/qsab126.

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Abstract Background The Thermo Scientific SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay and SureTect Escherichia coli STEC Identification PCR Assay are real-time PCR kits for the rapid detection of E. coli O157:H7 and non-E. coli O157 Shiga toxin-producing E. coli (STEC) serotypes (O26, O45, O103, O111, O121, O145) from fresh raw spinach, fresh baby leaves, fresh cut tomatoes, frozen raw beef, raw beef trim, and beef carcass sponges. Objective Both assays were evaluated for AOAC®Performance Tested MethodsSM certification. Methods Detection and confirmation inclusivity/exclusi

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Faulds, Nikki, Katharine Evans, Jessica Williams, et al. "Validation of the Thermo Scientific SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay and SureTect™ Escherichia coli STEC Identification PCR Assay for the Detection of Escherichia coli O157:H7 and the Escherichia coli STEC Serotypes (O26, O45, O103, O111, O121, O145) from Fresh Raw Spinach, Fresh Baby Leaves, Fresh Cut Tomatoes, Frozen Raw Beef, Raw Beef Trim, and Beef Carcass Sponges: AOAC Performance Tested MethodSM 012102." Journal of AOAC INTERNATIONAL 105, no. 2 (2021): 521–48. http://dx.doi.org/10.1093/jaoacint/qsab126.

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Abstract Background The Thermo Scientific SureTect™ Escherichia coli O157:H7 and STEC Screening PCR Assay and SureTect Escherichia coli STEC Identification PCR Assay are real-time PCR kits for the rapid detection of E. coli O157:H7 and non-E. coli O157 Shiga toxin-producing E. coli (STEC) serotypes (O26, O45, O103, O111, O121, O145) from fresh raw spinach, fresh baby leaves, fresh cut tomatoes, frozen raw beef, raw beef trim, and beef carcass sponges. Objective Both assays were evaluated for AOAC®Performance Tested MethodsSM certification. Methods Detection and confirmation inclusivity/exclusi

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Marin, J. M., R. P. Maluta, C. A. Borges, et al. "Fate of non O157 Shigatoxigenic Escherichia coli in ovine manure composting." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 66, no. 6 (2014): 1771–78. http://dx.doi.org/10.1590/1678-6001.

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Livestock manure may contain pathogenic microorganisms which pose a risk to the health of animal or humans if the manure is not adequately treated or disposed of. To determine the fate of Shiga toxigenic Escherichia coli (STEC) non O157 in composted manure from naturally colonized sheep, fresh manure was obtained from animals carrying bacterial cells with stx1/ stx2 genes. Two composting systems were used, aerated and non-aerated, and the experiments were done in Dracena city, São Paulo State. Every week, for seven weeks, one manure sample from six different points in both systems was collecte

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Qin, Xuan, Eileen J. Klein, Emmanouil Galanakis, et al. "Real-Time PCR Assay for Detection and Differentiation of Shiga Toxin-Producing Escherichia coli from Clinical Samples." Journal of Clinical Microbiology 53, no. 7 (2015): 2148–53. http://dx.doi.org/10.1128/jcm.00115-15.

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Timely accurate diagnosis of Shiga toxin-producingEscherichia coli(STEC) infections is important. We evaluated a laboratory-developed real-time PCR (LD-PCR) assay targetingstx1,stx2, andrfbEO157with 2,386 qualifying stool samples submitted to the microbiology laboratory of a tertiary care pediatric center between July 2011 and December 2013. Broth cultures of PCR-positive samples were tested for Shiga toxins by enzyme immunoassay (EIA) (ImmunoCard STAT! enterohemorrhagicE. coli[EHEC]; Meridian Bioscience) and cultured in attempts to recover both O157 and non-O157 STEC.E. coliO157 and non-O157

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Babolhavaeji, Kiandokht, Leili Shokoohizadeh, Morteza Yavari, Abbas Moradi, and Mohammad Yousef Alikhani. "Prevalence of Shiga Toxin-Producing Escherichia coli O157 and Non-O157 Serogroups Isolated from Fresh Raw Beef Meat Samples in an Industrial Slaughterhouse." International Journal of Microbiology 2021 (December 15, 2021): 1–6. http://dx.doi.org/10.1155/2021/1978952.

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Background. The aims of the current study are the identification of O157 and non-O157 Shiga Toxin-Producing Escherichia coli (STEC) serogroups isolated from fresh raw beef meat samples in an industrial slaughterhouse, determination of antimicrobial resistance patterns, and genetic linkage of STEC isolates. Materials and Methods. A total of 110 beef samples were collected from the depth of the rump of cattle slaughtered at Hamadan industrial slaughterhouse. After detection of E. coli isolates, STEC strains were identified according to PCR for stx1, stx2, eaeA, and hlyA virulence genes, and STEC

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PAOLI, GEORGE C., CHANDI WIJEY, and GAYLEN A. UHLICH. "Genetically Marked Strains of Shiga Toxin–Producing O157:H7 and Non-O157 Escherichia coli: Tools for Detection and Modeling†." Journal of Food Protection 78, no. 5 (2015): 888–901. http://dx.doi.org/10.4315/0362-028x.jfp-14-472.

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Shiga toxin–producing E. coli (STEC) is an important group of foodborne pathogens in the United States and worldwide. Nearly half of STEC-induced diarrheal disease in the United States is caused by serotype O157:H7, while non-O157 STEC account for the remaining illnesses. Thus, the U.S. Department of Agriculture (USDA) Food Safety and Inspection Service has instituted regulatory testing of beef products and has a zero-tolerance policy for regulatory samples that test positive for STEC O157:H7 and six other non-O157 STEC (serogroups O26, O45, O103, O111, O121, and O145). In this study, positive

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TSENG, M., Q. SHA, J. T. RUDRIK, et al. "Increasing incidence of non-O157 Shiga toxin-producing Escherichia coli (STEC) in Michigan and association with clinical illness." Epidemiology and Infection 144, no. 7 (2015): 1394–405. http://dx.doi.org/10.1017/s0950268815002836.

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SUMMARYInfection with Shiga toxin-producing Escherichia coli (STEC) by serotypes other than O157 (non-O157) have been increasingly reported in the United States. This increase in reporting is primarily due to the improvements in diagnostic tests. We analysed 1497 STEC cases reported in Michigan from 2001 to 2012. A significant increase in the number of non-O157 STEC cases was observed over time, and similar incidence rates were observed for O157 and non-O157 STEC cases in certain time periods. The odds of hospitalization was two times higher in O157 STEC cases relative to non-O157 STEC cases w

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Byrne, Lisa, Gemma L. Vanstone, Neil T. Perry, et al. "Epidemiology and microbiology of Shiga toxin-producing Escherichia coli other than serogroup O157 in England, 2009–2013." Journal of Medical Microbiology 63, no. 9 (2014): 1181–88. http://dx.doi.org/10.1099/jmm.0.075895-0.

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The implementation of direct testing of clinical faecal specimens for gastrointestinal (GI) pathogens by PCR offers a sensitive and comprehensive approach for the detection of Shiga toxin-producing Escherichia coli (STEC). The introduction of a commercial PCR assay, known as GI PCR, for the detection of GI pathogens at three frontline hospital laboratories in England between December 2012 and December 2013 led to a significant increase in detection of STEC other than serogroup O157 (non-O157 STEC). In 2013, 47 isolates were detected in England, compared with 57 in the preceding 4 years (2009–2

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Lonczynski, Thomas, and Laura Cowin. "The Validation of the SIMUL-qPCR Top 7 STEC Assay Collection: AOAC Performance Tested MethodSM 022001." Journal of AOAC INTERNATIONAL 104, no. 4 (2021): 1098–108. http://dx.doi.org/10.1093/jaoacint/qsab006.

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Abstract Background The Simultaneous Multiplex Real Time PCR (SIMUL-qPCR) Top 7 STEC Assay Collection is a quick, reliable method for detecting top seven Shiga toxin-producing Escherichia coli (STEC) in raw beef trim, raw ground beef, and beef carcass sampling sheets. Each assay multiplexes several targets in one run to identify E. coli O157: H7, O26, O45, O103, O111, O121, O145, Shiga toxin, and intimin genes. This collection uses specifically optimized proprietary media for single-step recovery and enrichment of enterohemorrhagic E. coli. Objective This report details the method validation s

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Tolen, Tamra, Yicheng Xie, Thomas Hairgrove, Jason Gill, and T. Taylor. "Evaluation of Commercial Prototype Bacteriophage Intervention Designed for Reducing O157 and Non-O157 Shiga-Toxigenic Escherichia coli (STEC) on Beef Cattle Hide." Foods 7, no. 7 (2018): 114. http://dx.doi.org/10.3390/foods7070114.

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Microbiological safety of beef products can be protected by application of antimicrobial interventions throughout the beef chain. This study evaluated a commercial prototype antimicrobial intervention comprised of lytic bacteriophages formulated to reduce O157 and non-O157 Shiga-toxigenic Escherichia coli (STEC) on beef cattle hide pieces, simulating commercial pre-harvest hide decontamination. STEC reduction in vitro by individual and cocktailed phages was determined by efficiency of plating (EOP). Following STEC inoculation onto hide pieces, the phage intervention was applied and hide pieces

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Tanyitiku, Mary Nkongho, Graeme Nicholas, Jon J. Sullivan, Igor C. Njombissie Petcheu, and Stephen L. W. On. "Survival of Escherichia coli in Edible Land Snails: Implications for Heliciculture and Public Health." Pathogens 13, no. 3 (2024): 204. http://dx.doi.org/10.3390/pathogens13030204.

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Background: Land snails are considered a delicacy in many countries in Europe and sub-Saharan Africa. However, the interaction of microbial pathogens with land snails may present a public health threat when handling and/or consuming snails. This study examines the survival of Escherichia coli in edible land snails in a model system. Methods: Well-studied Shigatoxigenic (STEC) and non-STEC strains were compared. Mature Helix spp. were experimentally fed with E. coli-inoculated oats for 48 h. The snail feces after inoculation were periodically sampled and cultured for a 30-day period and subject

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CELIK, G., A. DIKICI, and A. KOLUMAN. "Survival of Shiga Toxin-Producing Escherichia coli (STEC) Serogroups During Production and Storage of Yogurt." Journal of the Hellenic Veterinary Medical Society 72, no. 1 (2021): 2689. http://dx.doi.org/10.12681/jhvms.26753.

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In this study, the survival of Escherichia coli O157:H7 and non-O157 STEC serogroups of O26, O111, O103, and O145 were investigated during production and storage of yogurt. For this purpose, pathogens were individually inoculated into milk after pasteurization along with the starter culture (approximately 7.00±1.00 log10 cfu/g). After incubation at 44oC (about 180 min), yogurt samples were capped and stored at 4oC for 20 days. Pathogens were enumerated at 0, 5, 10, 15, and 20th days of storage. Lactic acid content (%) and pH of the samples were also screened. Moreover, mesophilic Lactococcus s

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Arthur, Terrance M., Genevieve A. Barkocy-Gallagher, Mildred Rivera-Betancourt, and Mohammad Koohmaraie. "Prevalence and Characterization of Non-O157 Shiga Toxin-Producing Escherichia coli on Carcasses in Commercial Beef Cattle Processing Plants." Applied and Environmental Microbiology 68, no. 10 (2002): 4847–52. http://dx.doi.org/10.1128/aem.68.10.4847-4852.2002.

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ABSTRACT Beef carcass sponge samples collected from July to August 1999 at four large processing plants in the United States were surveyed for the presence of non-O157 Shiga toxin-producing Escherichia coli (STEC). Twenty-eight (93%) of 30 single-source lots surveyed included at least one sample containing non-O157 STEC. Of 334 carcasses sampled prior to evisceration, 180 (54%) were found to harbor non-O157 STEC. Non-O157 STEC isolates were also recovered from 27 (8%) of 326 carcasses sampled after the application of antimicrobial interventions. Altogether, 361 non-O157 STEC isolates, comprisi

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Islam, Mohammad A., Abdus S. Mondol, Enne de Boer, et al. "Prevalence and Genetic Characterization of Shiga Toxin-Producing Escherichia coli Isolates from Slaughtered Animals in Bangladesh." Applied and Environmental Microbiology 74, no. 17 (2008): 5414–21. http://dx.doi.org/10.1128/aem.00854-08.

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ABSTRACT To determine the prevalence of Shiga toxin (Stx)-producing Escherichia coli (STEC) in slaughter animals in Dhaka, Bangladesh, we collected rectal contents immediately after animals were slaughtered. Of the samples collected from buffalo (n = 174), cows (n = 139), and goats (n = 110), 82.2%, 72.7%, and 11.8% tested positive for stx 1 and/or stx 2, respectively. STEC could be isolated from 37.9%, 20.1%, and 10.0% of the buffalo, cows, and goats, respectively. STEC O157 samples were isolated from 14.4% of the buffalo, 7.2% of the cows, and 9.1% of the goats. More than 93% (n = 42) of the

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Ismaili, Arif, Elaine McWhirter, Michelle Y. C. Handelsman, James L. Brunton, and Philip M. Sherman. "Divergent Signal Transduction Responses to Infection with Attaching and Effacing Escherichia coli." Infection and Immunity 66, no. 4 (1998): 1688–96. http://dx.doi.org/10.1128/iai.66.4.1688-1696.1998.

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ABSTRACT Shiga toxin-producing Escherichia coli (STEC) O157:H7 is an attaching and effacing pathogen that causes hemorrhagic colitis and the hemolytic-uremic syndrome. Although this organism causes adhesion pedestals, the cellular signals responsible for the formation of these lesions have not been clearly defined. We have shown previously that STEC O157:H7 does not induce detectable tyrosine phosphorylation of host cell proteins upon binding to eukaryotic cells and is not internalized into nonphagocytic epithelial cells. In the present study, tyrosine-phosphorylated proteins were detected und

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Hajra, Tapas K., Prasanta K. Bag, Suresh C. Das, Souryadeep Mukherjee, Asis Khan, and T. Ramamurthy. "Development of a Simple Latex Agglutination Assay for Detection of Shiga Toxin-Producing Escherichia coli (STEC) by Using Polyclonal Antibody against STEC." Clinical and Vaccine Immunology 14, no. 5 (2007): 600–604. http://dx.doi.org/10.1128/cvi.00342-06.

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ABSTRACT Rabbit antiserum raised against the whole-cell antigen of Shiga toxin-producing Escherichia coli (STEC) strain VT3 (stx 1 + stx 2 + eae +) was repeatedly adsorbed with heat-killed cells of different non-STEC strains and other enteric bacteria. Thus, the antiserum obtained was designated VT3 antiserum. VT3 antiserum reacted with intimin type γ. We assessed the reactivity of VT3 antiserum to whole-cell lysates of 87 strains of E. coli and other enteric bacteria by immunoblotting. The antiserum recognized the 97-kDa protein in whole-cell lysate from strain VT3, and 36 (83.7%) of the 43 S

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GLASS, KATHLEEN A., CHARLES W. KASPAR, JEFFREY J. SINDELAR, et al. "Validation of Pepperoni Process for Control of Shiga Toxin–Producing Escherichia coli." Journal of Food Protection 75, no. 5 (2012): 838–46. http://dx.doi.org/10.4315/0362-028x.jfp-11-486.

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The objective of this study was to compare the survival of non-O157 Shiga toxin–producing Escherichia coli (STEC) with E. coli O157:H7 during pepperoni production. Pepperoni batter was inoculated with 7 log CFU/g of a seven-strain STEC mixture, including strains of serotypes O26, O45, O103, O111, O121, O145, and O157. Sausages were fermented to pH ≤4.8, heated at 53.3°C for 1 h, and dried for up to 20 days. STEC strains were enumerated at designated intervals on sorbitol MacConkey (SMAC) and Rainbow (RA) agars; enrichments were completed in modified EC (mEC) broth and nonselective tryptic soy

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EKIRI, ABEL B., DOUGLAS LANDBLOM, DAWN DOETKOTT, SUSAN OLET, WEILIN L. SHELVER, and MARGARET L. KHAITSA. "Isolation and Characterization of Shiga Toxin–Producing Escherichia coli Serogroups O26, O45, O103, O111, O113, O121, O145, and O157 Shed from Range and Feedlot Cattle from Postweaning to Slaughter." Journal of Food Protection 77, no. 7 (2014): 1052–61. http://dx.doi.org/10.4315/0362-028x.jfp-13-373.

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Cattle are the main reservoirs for Shiga toxin–producing Escherichia coli (STEC) strains. E. coli O26, O45, O103, O111, O121, O145, and O157 are among the STEC serogroups that cause severe foodborne illness and have been declared as adulterants by the U.S. Department of Agriculture, Food Safety and Inspection Service. The objectives of this study were (i) to estimate the prevalence of non-O157 STEC and E. coli O157 in naturally infected beef cows and in steer calves at postweaning, during finishing, and at slaughter and (ii) to test non-O157 STEC isolates for the presence of virulence genes st

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Chui, Linda, Vincent Li, Patrick Fach, et al. "Molecular Profiling of Escherichia coli O157:H7 and Non-O157 Strains Isolated from Humans and Cattle in Alberta, Canada." Journal of Clinical Microbiology 53, no. 3 (2014): 986–90. http://dx.doi.org/10.1128/jcm.03321-14.

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Virulence markers in Shiga toxin-producingEscherichia coli(STEC) and their association with diseases remain largely unknown. This study determines the importance of 44 genetic markers for STEC (O157 and non-O157) from human clinical cases and their correlation to disease outcome. STEC isolated from a cattle surveillance program were also included. The virulence genes tested were present in almost all O157:H7 isolates but highly variable in non-O157 STEC isolates. Patient age was a significant determinant of clinical outcome.

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Elmonir, Walid, Etab Mohamed Abo Remela, and Yasmine Alwakil. "Diversity, virulence and antibiogram traits of Escherichia coli recovered from potable water sources in Gharbia, Egypt." Journal of Water and Health 18, no. 3 (2020): 430–38. http://dx.doi.org/10.2166/wh.2020.239.

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Abstract This study aimed to assess the public health risk of coliforms and Escherichia coli contamination of potable water sources in Egypt. A total of 150 water samples (100 tap and 50 well) were collected from five districts in Gharbia governorate, Egypt. High rates of coliforms contamination were recorded in 52 and 76% of examined tap and well water samples, respectively. E. coli strains were detected in 16% of the water samples (15% tap water and 18% well water; 23.7% rural and 8.1% urban). Rural water sources were 3.5 times more likely to be contaminated than urban sources (P = 0.01). Ei

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GÓMEZ-ALDAPA, CARLOS A., ESMERALDA RANGEL-VARGAS, M. del REFUGIO TORRES-VITELA, ANGÉLICA VILLARRUEL-LÓPEZ, and JAVIER CASTRO-ROSAS. "Behavior of Non-O157 Shiga Toxin–Producing Escherichia coli, Enteroinvasive E. coli, Enteropathogenic E. coli, and Enterotoxigenic E. coli Strains on Alfalfa Sprouts." Journal of Food Protection 76, no. 8 (2013): 1429–33. http://dx.doi.org/10.4315/0362-028x.jfp-13-060.

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Data about the behavior of non-O157 Shiga toxin–producing Escherichia coli (non-O157 STEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC), and enteropathogenic E. coli (EPEC) on seeds and alfalfa sprouts are not available. The behavior of STEC, EIEC, ETEC, and EPEC was determined during germination and sprouting of alfalfa seeds at 20 ± 2°C and 30 ± 2°C and on alfalfa sprouts at 3 ± 2°C. When alfalfa seeds were inoculated with STEC, EIEC, ETEC, or EPEC strains, all these diarrheagenic E. coli pathotypes (DEPs) grew during germination and sprouting of seeds, reaching counts of a

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