Dissertations / Theses on the topic 'Non-specific binding'
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Shedge, Hemangi Y. "Specific and non-specific binding of proteins and nucleic acids on chemically modified reticulated vitreous carbon electrodes." Connect to this title online, 2009. http://etd.lib.clemson.edu/documents/1246559605/.
Full textMcLure, James Alexander, and james mclure@flinders edu au. "Physicochemical determinants of the non-specific binding of drugs to human liver microsomes." Flinders University. Medicine, 2008. http://catalogue.flinders.edu.au./local/adt/public/adt-SFU20081102.165952.
Full textSlowski, Kathryn Johanna. "Identification of a novel microRNA involved in non-specific binding to a decoy transcript." Thesis, University of British Columbia, 2016. http://hdl.handle.net/2429/59879.
Full textMedicine, Faculty of
Graduate
Child, Chloe Rose. "Determing structure-activity relationships between novel PET radiotracers and their non-specific binding properties." Thesis, Imperial College London, 2012. http://hdl.handle.net/10044/1/9576.
Full textWesterhult, David. "Weak Affinity Chromatography : Evaluation of Different Silica Supports for Protein Immobilizationand Effect of Mobile Phases Regarding Retentionand Non-specific Binding." Thesis, Linnéuniversitetet, Institutionen för naturvetenskap, NV, 2012. http://urn.kb.se/resolve?urn=urn:nbn:se:lnu:diva-17968.
Full textFouquier, d'Herouel Aymeric. "Statistical models of TF/DNA interaction." Licentiate thesis, Stockholm : Numerisk analys och datalogi, Numerical Analysis and Computer Science, Kungliga Tekniska högskolan, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-4633.
Full textCowsill, Benjamin James. "The physics of pregnancy tests : a biophysical study of interfacial protein adsorption." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/the-physics-of-pregnancy-tests-a-biophysical-study-of-interfacial-protein-adsorption(538b8e9c-9111-4eb9-ac7d-e5e75110e315).html.
Full textRichardson, Mandek. "Theoretical and Experimental Investigations to Improve the Performance of Surface Acoustic Wave (SAW) Biosensors." Scholar Commons, 2014. https://scholarcommons.usf.edu/etd/5566.
Full textVasquez, Colins. "Allosteric effects of the GTP-specific binding site and a benzimidazole-derivative non-nucleoside inhibitor on the hepatitis C virus RNA-dependent RNA polymerase." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40750.
Full textLe virus de l’hépatite C (VHC) est un virus d’ARN de polarité positive qui appartient à la famille Flaviviridae. Le VHC exprime la protéine non-structurelle NS5B, une ARN polymérase dépendante de l’ARN indispensable à la réplication du génome virale. Nous décrivons ici les propriétés allostériques du site GTP-spécifique (site-G) du VHC polymérase, ainsi que les propriétés allostériques de VIR-1327, un inhibiteur non-nucléoside (INN) qui est dérivé du benzimidazole. En utilisant des tests biochimiques, nous avons identifié certains résidus du site-G qui influencent l’activité du NS5B, incluant sérine-29, proline-495 et valine-499. Nous avons également démontré que VIR-1327 est un puissant inhibiteur avec un profil de résistance comprenant les mutants P495A et V499A, mais qu’il est non-compétitif avec le GTP. De plus, des résultats biophysiques suggèrent que le méchanisme d’action de VIR-1327 est de prévenir la formation d’un complexe enzyme-matrice stable. D’autres analyses du site-G et des INN vont non seulement fournir une meilleure compréhension de la réplication du VHC, mais aussi valider différents sites allostériques comme étant des cibles virus-spécifiques pour le développement de thérapeutiques contre le VHC.
Engqvist, Martin. "A generic capture assay for immunogenicity, using Biacore." Thesis, Uppsala universitet, Institutionen för biologisk grundutbildning, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-198767.
Full textBodenstein, Johannes. "Antagonism by selected classical irreversible competitive antagonists : an investigation into the proposed non-specific mechanisms involved / Johannes Bodenstein." Thesis, North-West University, 2003. http://hdl.handle.net/10394/92.
Full textThesis (Ph.D. (Pharmacology))--North-West University, Potchefstroom Campus, 2004.
Long, Jhuo Jheng, and 卓政隆. "Effect of Longitudinal Ultrasound Acting on Specific and Non-Specific Binding Particles." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/70354793328792672023.
Full text國立中正大學
機械系
99
In biochemistry detection, the analysis usually includes two kinds of particles, one are specific absorption particles which will react with the detection area, another are non-specific absorption particles which will not react with the detection area. However, non-specific absorption particles will bind the detection area with Van der Waal Force, causing the result of detection error. Apply high frequency electric field on PZT, which is the piezoelectric material, will produce an ultrasonic wave. Apply ultrasonic wave on the detection area can remove non-specific absorption particles, makes specific absorption particles react with the detection area. Besides, apply ultrasonic wave on the detection area under the condition that the analysis only contain specific absorption particles, can increase the number of specific absorption particles that react with the detection area.
McLure, James Alexander. "Physicochemical determinants of the non-specific binding of drugs to human liver microsomes." 2008. http://catalogue.flinders.edu.au/local/adt/public/adt-SFU20081102.165952/index.html.
Full textGanguly, Abantika. "Probing Macromolecular Reactions At Reduced Dimensionality : Mapping Of Sequence Specific And Non-Specific Protein-Ligand lnteractions." Thesis, 2012. http://etd.iisc.ernet.in/handle/2005/2478.
Full textHuang, Chun-Chin, and 黃俊欽. "Prediction of Transcription Factor Domain based on Analysis of Specific and non-Specific DNA-Binding Residues on the Protein Sequence." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/52594622165933883244.
Full text國立臺灣大學
工程科學及海洋工程學研究所
97
Protein-DNA interactions are essential for fundamental biochemical activities including DNA transcription, replication, packaging, repair and rearrangement. Proteins interacting with DNA can be classified into two modes distinguished by sequence-specific and non-specific binding respectively. Protein-DNA specific binding provides a mechanism to recognize correct nucleotide base pairs namely sequence-specific identification. On the other hand, protein-DNA non-specific binding shows relatively little base-sequence preference and interacts with DNA backbone. In this thesis, we present a two stage Protein-DNA binding prediction. In the first stage of DNA-binding residues prediction, the predictor for DNA specific binding residues achieves 96.45% accuracy with 50.14% sensitivity, 99.31% specificity, 81.70% precision, and 62.15% F-measure. The predictor for DNA non-specific binding residues achieves 89.14% accuracy with 53.06% sensitivity, 95.25% specificity, 65.47% precision, and 58.62% F-measure. In addition, we combine the results of sequence-specific and non-specific binding residues predicted in previous stage with OR operation, and the predictor achieves 89.26% accuracy with 56.86% sensitivity, 95.63% specificity, 71.92% precision, and 63.51% F-measure. In the second stage, a protein-DNA interaction mode predictor is proposed. It can achieve 75.83% accuracy while using support vector machine with multi-class prediction. This article presents the design of a sequence-based predictor aiming to identify the sequence-specific and non-specific DNA-binding residues in a transcription factor with DNA binding-mechanism concerned. The protein-DNA interaction mode prediction was introduced to provide biochemist more structural hint and help improve previous DNA-binding residues prediction. In addition, we will exploit the experiences learned in this study to design binding-mechanism concerned predictors for other types of DNA-contacted proteins.
"Exploring the Nature of Protein-Peptide Interactions on Surfaces." Doctoral diss., 2014. http://hdl.handle.net/2286/R.I.25814.
Full textDissertation/Thesis
Doctoral Dissertation Biochemistry 2014
Swartz, Ellen Ashley. "Cell specific transcriptional regulation of the smooth muscle [alpha]-actin gene promoter : two M-CAT elements have differences in functionalactivity and binding properties in smooth muscle versus non-smooth muscle cells /." 1997. http://wwwlib.umi.com/dissertations/fullcit/9724670.
Full textSpine title: M-CAT motifs of the SM [alpha]-actin gene. Includes bibliographical references (90-100). Also available online through Digital Dissertations.
Swartz, Ellen Ashley. "Cell specific transcriptional regulation of the smooth muscle [alpha]-actin gene promotoer : two m-cat elements have differences in functional activity and binding properties in smooth muscle versus non-smooth muscle cells /." 1997. http://wwwlib.umi.com/dissertations/fullcit/9724670.
Full textHolman, Garen Gilman. "Binding studies of a sequence specific threading NDI intercalator." Thesis, 2011. http://hdl.handle.net/2152/ETD-UT-2011-08-3863.
Full texttext
Breault-Turcot, Julien. "Développement d’outils analytiques pour la détection de biomolécules directement dans des fluides sanguins." Thèse, 2015. http://hdl.handle.net/1866/13566.
Full textThis thesis discusses the development of surface plasmon resonnance (SPR) biosensors to perform detection directly on unpurified and undiluted blood based fluids such as serum or blood. Every biosensor discussed in the following chapters rely on a home-built portable SPR device developed in Professor Masson’s research laboratories. Non-specific adsorption, which greatly hinders biosensing in crude fluids, will be the first topic of the thesis. Serum adsorption was performed on the SPR sensor surface and then characterized by SPR and mass spectrometry. This study provided useful information for biosensing directly in blood-based fluids. It also provided a better fundamental understanding of the nonspecific adsorption process on surfaces. The first biosensor was developed to detect prostate specific antigen (PSA), a protein normally contained in serum, which is a known biomarker for prostate cancer. In order to detect low concentrations of this protein directly in serum, a microstructured gold film was used to amplify the signal generated by the binding event on the biosensor. A peptide monolayer covered the metallic surface of the sensor to reduce non-specific protein adsorption. The SPR portable instrument was modified to simultaneous detect fluorescence in order to perform a SPR and ELISA test in a single instrumental platform. Each technique provided a control for the other for detection of the prostate cancer biomarker at concentration levels required for the screening of the disease. The SPR and ELISA combination also extended the dynamic range of the biosensing assay. Finally, the portable SPR device was used to detect small biomolecules with potential therapeutic activity directly in a sample of blood. Peptides with an anti-atherosclerotic activity were thus detected in an unpurified and undiluted blood sample at micromolar concentration. The addition of a porous membrane to the microfluidic used for the biosensing assay facilitated the successful detection of these molecules in whole blood. The present thesis describes novel strategies and instrumental modifications to unlock the possibility of performing biosensing directly on unpurified and undiluted blood-based fluids. Modifications of the fluidic system, the SPR instrument and biosensor used will allow detection in fluids with high complexity such as serum or blood. The work described herein reports a prostate cancer screening assay performed in 12 minutes directly in serum using a portable SPR/fluorescence instrument. Finally, this thesis reports one of the first scientific papers where a SPR biosensor is used to perform analysis directly in blood.
Gregory, Kasimir Phennah. "A quantum chemical investigation of Hofmeister effects in non-aqueous solvents." Thesis, 2022. http://hdl.handle.net/1959.13/1460595.
Full textSpecific ion effects (SIEs) encompass any phenomenon induced by ions that is dependent on the identity of the ions, and not just their charge or concentration. These occur in salts, electrolyte solutions, ionic liquids, acids and bases and have been known for over 130 years, from which the Hofmeister series originated. They are important in biology, nutrition, electrochemistry and various interfacial or geophysico-phenomena. It is perhaps harder to find a “real-world” system in which specific ion effects don’t occur, than systems where they do. Nonetheless, despite such ubiquity and effect on our daily lives, our understanding of these salty solutions is limited. This thesis addresses the knowledge gap surrounding the lack of parameters (for both ion and solvent) for quantifying SIEs in aqueous and nonaqueous environments. This thesis begins with a deeper introduction to the topic of SIEs and highlights the current state-of-play. The theories underlying quantum mechanics and computational chemistry are discussed to highlight how they may be applied to elucidate some of the fundamental origins of SIEs. These methods were subsequently used to investigate possible energetic origins of counterion and solvent induced reversals to the Hofmeister series, and highlights that the Lewis acidity and basicity (collectively Lewis strength) indices of the cations and anions respectively, can quantify SIEs. Following this revelation, these empirical parameters were recast in terms of intermolecular forces. Electrostatics appeared to govern the Lewis strength indices, so these were replaced with an electrostatic parameter, ϸ (“sho”), that originates from Coulomb’s Law. For anions, ϸ is shown to quantify SIE trends observed in enthalpies of hydration, polymer lower critical solution temperatures, enzyme and viral activities, SN2 reaction rates and Gibbs free energies of transfer from water to nonaqueous solvents highlighting the versatility of ϸ as a new SIE parameter. Cation interactions are more prone to deviations from ϸ correlations. In the absence of any cosolute (i.e., pure ion-solvent interactions) however, cation solvent interactions follow a strong trend with Coulomb’s Law for ~15 different solvents. This supports a conclusion that competing electrostatic interactions between the solvent and a cosolute for the cation may mask each other allowing non-electrostatic contributions to play a dominant role. Furthermore, with similarity to the ion parameterisation, the ϸ values at the negative and positive solvent dipolar atoms correlate with the solvent’s Lewis basicity and acidity respectively. Additionally, these analyses can be related to macroscopic solvent parameters such as the relative permittivity. The data deficiency issue facing the SIE field was more generally addressed in this thesis by the generation of IonSolvR, a repository containing over 3000 distinct QM/MD trajectories of up to 52 ions in 28 bulk solvents on nanosecond-scales. Finally, the key findings of this thesis are summarised and an outlook on the field of SIEs and the broader implications arising from this thesis is presented.