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Published: 4 June 2021

Last updated: 21 February 2023

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1

Riffkin, Penelope A. "An assessment of white clover nitrogen fixation in grazed dairy pastures of south-western Victoria." Thesis, [Richmond, N.S.W.] : University of Western Sydney, Hawkesbury, 1999. http://handle.uws.edu.au:8081/1959.7/31.

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Australia is amongst the more efficient milk producers in the world.Milk production in the region of south-western Victoria relies mainly on rainfed white clover/perennial ryegrass pastures.As the demand for efficient and competitive milk production increases, the value of N2 fixation must be maximised. The objective of this thesis was to assess N2 fixation in grazed dairy pastures in south-western Victoria. Several tests and experiments were conducted and results noted. Studies revealed low white clover yields to be the major factor limiting N2 fixation in the region. For N2 fixation to have a significant impact on pasture quality and production, problems associated with legume persistence need to be addressed. Strategies may include the breeding of white clover cultivars with greater tolerance to water stress, improved winter production and increased competitiveness with companion species. Alternatively, the introduction of different legume species, better suited to the environment, may be appropriate. Where N2 fixation is unlikely to satisfy N demands, it may be necessary to introduce the strategic use of supplementary feeds or nitrogenous fertilisers. However, this would need to be carefully considered to ensure high input costs did not jeopardise the competitive advantage of low input pasture-based systems

2

Riffkin, Penelope A., of Western Sydney Hawkesbury University, and Faculty of Science and Technology. "An assessment of white clover nitrogen fixation in grazed dairy pastures of south-western Victoria." THESIS_FST_xxx_Riffkin_P.xml, 1999. http://handle.uws.edu.au:8081/1959.7/31.

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Australia is amongst the more efficient milk producers in the world.Milk production in the region of south-western Victoria relies mainly on rainfed white clover/perennial ryegrass pastures.As the demand for efficient and competitive milk production increases, the value of N2 fixation must be maximised. The objective of this thesis was to assess N2 fixation in grazed dairy pastures in south-western Victoria. Several tests and experiments were conducted and results noted. Studies revealed low white clover yields to be the major factor limiting N2 fixation in the region. For N2 fixation to have a significant impact on pasture quality and production, problems associated with legume persistence need to be addressed. Strategies may include the breeding of white clover cultivars with greater tolerance to water stress, improved winter production and increased competitiveness with companion species. Alternatively, the introduction of different legume species, better suited to the environment, may be appropriate. Where N2 fixation is unlikely to satisfy N demands, it may be necessary to introduce the strategic use of supplementary feeds or nitrogenous fertilisers. However, this would need to be carefully considered to ensure high input costs did not jeopardise the competitive advantage of low input pasture-based systems
Masters Thesis

3

Cho, Hyojin. "A study of transcript profiling of soybean roots during nitrogen fixing symbiosis." Diss., Columbia, Mo. : University of Missouri-Columbia, 2006. http://hdl.handle.net/10355/5915.

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Thesis (M.S.)--University of Missouri-Columbia, 2006.
The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on May 7, 2009) Includes bibliographical references.

4

He, Xinhua. "Nitrogen exchange between plants through common mycorrhizal networks /." [St. Lucia, Qld.], 2002. http://www.library.uq.edu.au/pdfserve.php?image=thesisabs/absthe18272.pdf.

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5

Devkota, Dibya. "Habitat, isolation, identification and nitrogen fixation of Rhizobiaceae associated with rangeland legumes from Wyoming, USA." Laramie, Wyo. : University of Wyoming, 2007. http://proquest.umi.com/pqdweb?did=1313917311&sid=1&Fmt=2&clientId=18949&RQT=309&VName=PQD.

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6

Rana, Debashis. "The nitrogen-fixing symbiosis between Rhizobium sp. sin-1 and Sesbania spp. /." free to MU campus, to others for purchase, 1997. http://wwwlib.umi.com/cr/mo/fullcit?p9842559.

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7

Burn, Joanne Elizabeth. "Analysis of the regulatory nodulation gene nodD of rhizobium leguminosarum." Thesis, University of East Anglia, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.329095.

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8

Miller, Simon Hugh, and n/a. "Genetic basis for the host-specific nitrogen fixation phenotype of Caucasian clover rhizobia." University of Otago. Department of Microbiology, 2006. http://adt.otago.ac.nz./public/adt-NZDU20070306.155157.

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Trifolium ambiguum (Caucasian clover) is being released in New Zealand for use in areas where growth of T. repens (white clover) is marginal. Although closely related to T. repens, T. ambiguum has unique and highly specific nodulation requirements and as rhizobial strains capable of effectively nodulating T. ambiguum are not naturally found in New Zealand soils, they must be introduced with the seed. Rhizobium leguminosarum bv. trifolii strains such as ICC105 form effective nodules on T. ambiguum but ineffective (Fix⁻) nodules on T. repens. The T. repens nodules nevertheless develop normally and contain bacteroids. R. l. bv. trifolii strains that are effective on T. repens such as NZP561, fail to nodulate T. ambiguum. As the host-specific nitrogen fixation defect of Caucasian clover rhizobia on T. repens has potentially adverse agronomic implications, the genetic basis for this Fix⁻ phenotype was investigated. Rhizobium leguminosarum bv. trifolii strain ICC105 was converted to Fix⁺ on T. repens by the introduction of an 18-kb fragment of DNA from a white clover rhizobial strain (NZP514) symbiotic plasmid. This fragment contained several nif and fix genes, including nifHDKEN, fixABCX, nifA, nifB, fdxN and fixU. Tn5 mutation of these white clover rhizobial genes demonstrated that most were required to impart the Fix⁺ phenotype on T. repens to ICC105, with the exception of nifA. Mutagenesis of the ICC105 nifA gene and subsequent complementation with various combinations of the white clover rhizobia nif/fix genes as well as transcriptional lacZ fusion studies of the ICC105 nifA and nifH genes demonstrated that ICC105 nifA is expressed and functional during the ineffective nodulation of T. repens and able to activate expression of nifHDKEN and fixABCX operons derived from white clover rhizobium but not from ICC105. Sequence analysis and comparison of the intergenic region between the divergently transcribed nif/fix operons revealed a conserved 111-bp region found between the nifH/fixA promoters of Caucasian clover rhizobia, but not in white clover rhizobia. Attempts to modify this region in ICC105 failed in creating a strain which was Fix⁺ on T. repens; however recombination of the nifHD/fixAB region from a white clover rhizobium into the ICC105 genome produced several strains with a �swapped� nitrogen fixation phenotype (i.e. Fix⁺ on T. repens and Fix⁻ on T. ambiguum). A hypothesis was therefore proposed by which differences in the nifH/fixA promoter regions of Caucasian clover rhizobia and white clover rhizobia modulate the expression of the upstream genes in response to the particular plant host they are nodulating. The incompatibility between the symbiotic plasmid of R. l. bv. trifolii ICC105 and the white clover rhizobium symbiotic plasmid cointegrate, pPN1, was also investigated and potential regions of each plasmid involved in this incompatibility were identified. The research presented in this thesis has contributed to the genetic knowledge of the nitrogen fixation genes, and regulation of these genes in R. l. bv. trifolii. It has also provided progress towards the goal of creating a suitable inoculant strain for T. ambiguum that is able to fix nitrogen in symbiosis with both T. repens and T. ambiguum.

9

Abi-Ghanem, Rita. "Optimizing biological nitrogen fixation and evaluating Iraqi extension education." Pullman, Wash. : Washington State University, 2009. http://www.dissertations.wsu.edu/Dissertations/Summer2009/R_Abi-Ghanem_070909.pdf.

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10

Thorne, Stephen Howard. "Stationary phase survival of Rhizobium leguminosarum." Thesis, Imperial College London, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.265401.

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11

Burgoyne, Tricia A. "Free living nitrogen-fixation in ponderosa pine/Douglas-fir forests of western Montana." Connect to this title online, 2007. http://etd.lib.umt.edu/theses/available/etd-05302007-085002/.

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12

Riffkin, Penelope A. "An assessment of white clover nitrogen fixation in grazed dairy pastures of South-Western Victoria /." [Richmond, N.S.W.] : University of Western Sydney, Hawkesbury, 1999. http://library.uws.edu.au/adt-NUWS/public/adt-NUWS20030528.152118/index.html.

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13

Shah, Ritu. "Role of bacterial NADP dependent isocitrate dehydrogenase in the Bradyrhizobium japonicum and soybean symbiosis /." free to MU campus, to others for purchase, 2003. http://wwwlib.umi.com/cr/mo/fullcit?p3115604.

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14

Ditsch, David C. "Fate of ¹⁵N-depleted fertilizer N in a corn-rye cropping sequence: plant uptake and soil distribution." Diss., Virginia Tech, 1991. http://hdl.handle.net/10919/37238.

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15

Mirza, Babur S. "Saprophytic growth and fate of Frankia strains in soil /." View online, 2009. http://ecommons.txstate.edu/dissertations/AAI3384734/.

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16

Lotfollahi, Mohammad. "The effect of subsoil mineral nitrogen on grain protein concentration of wheat." Title page, table of contents and summary only, 1996. http://web4.library.adelaide.edu.au/theses/09PH/09phl882.pdf.

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Copy of author's previously published work inserted. Bibliography: leaves 147-189. This project examines the uptake of mineral N from the subsoil after anthesis and its effect on grain protein concentration (GPC) of wheat. The overall objective is to examine the importance of subsoil mineral N and to investigate the ability of wheat to take up N from the subsoil late in the season under different conditions of N supply and soil water availability. Greenhouse experiments investigate the importance of subsoil mineral N availability on GPC of wheat and the factors that contribute to the effective utilisation of N. The recovery of N from subsoil, the effect of split N application on GPC and short term N uptake by the wheat at different rooting densities are also studied.

17

Qu, Wenchuan. "Studies on nitrogen cycling processes in Lake Illawarra, New South Wales, Australia." Access electronically, 2004. http://www.library.uow.edu.au/adt-NWU/public/adt-NWU20050302.161641/index.html.

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18

Berdugo, Silvia Eugenia Barrera. "Fixação biológica de N2 e diversidade de bactérias diazotroficas numa Floresta de Restinga." Universidade de São Paulo, 2012. http://www.teses.usp.br/teses/disponiveis/11/11140/tde-20092012-101012/.

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Diazotróficos de vida-livre podem ser encontradas associadas à filosfera, dermosfera e rizosfera das espécies vegetais. Alguns dados sugerem que a fixação biológica de N2 (FBN) por bactérias assimbióticas representa uma entrada importante de nitrogênio nos ecossistemas tropicais, variando com as espécies vegetais e nas diferentes partes da planta. O presente trabalho teve como objetivos estimar a quantidade de N2 fixado de forma assimbiótica na filosfera, dermosfera e rizosfera sobre a copa das espécies vegetais Guapira oposita e Euterpe edulis, e avaliar a diversidade das bactérias assimbióticas, através da análise do gene rRNA 16S, em uma Restinga ,em Ubatuba, SP. O estudo foi realizado no Parque Estadual da Serra do Mar, Núcleo Picinguaba, em épocas de baixa e alta pluviosidade. A atividade da nitrogenase foi determinada pela técnica de redução do acetileno e as concentrações de etileno foram determinadas por cromatografia gasosa. A diversidade de bactérias que habitam filosfera, dermosfera e solo foi acessada por pirosequenciamento da região V4 do gene rRNA 16S. A maior fixação de N foi observada na dermosfera de E. edulis nas duas épocas de coleta (175,1± 53,4 ng cm-2 h-1; 97,2 ± 21 ng cm-2 h-1), as taxas de fixação de N mais baixas foram observadas no solo. Na época de alta pluviosidade, a FBN na filosfera de G. oposita (52,0 ± 12 ng cm-2 h-1) foi significativamente maior do que a filosfera de E. edulis (3,6 ± 06 ng cm-2. h-1) e do que no mesmo compartimento mas em diferentes épocas de coleta (7,5 ± 1,3 ng cm-2 h-1). O valor do 15N foi maior no solo onde a fixação de N foi mais baixa. Na filosfera e na dermosfera, a relação C/N foi mais baixa quando a FBN foi mais alta. A FBN no solo e serrapilheira de restinga apresentou grande variação espacial, com locais de alta atividade. As 188629 sequências obtidas foram agrupadas em 16727 Unidades Taxonômicas Operacionais (UTOs), distribuídos em 35 filos. Os principais filos detectados foram Proteobacteria (38%) e Acidobacteria (12%). As classes Alphaproteobacteria e Gammaproteobacteria foram as mais abundantes nos três compartimentos. Potenciais fixadores de N foram detectados nas classes Alpha Beta e Gammaproteobacteria. A abundância de cianobacterias fixadoras de N na filosfera e na dermosfera foi baixa, indicando que outros diazotróficos também colonizam esses ambientes e contribuem com a FBN.
Free-living N2 fixing bacteria can be found associated with the phyllosphere, bark and rizosphere of the diferent plant species. Some data suggest that biological N2 fixation (BNF) by free-living bacteria represents an important input of nitrogen in tropical ecosystem, varying with the plant species and in different parts of the plant. This study aimed to estimate the amount of N2 fixed in the phyllosphere, bark and soil under the canopy of Guapira opposite and Euterpe edullis, and evaluate the diversity of bacteria through the sequencing of the 16S rRNA gene analysis, the phyllosphere, bark and soil in a Restinga area, Ubatuba, SP. The study was conducted in the Parque Estadual da Serra do Mar, Núcleo Picinguaba in seasons of low and high rainfall. Nitrogenase activity was determined by the acetylene reduction assay (ARA) and ethylene concentrations were determined by gas chromatography. The diversity of bacteria in the phyllosphere, bark and soil was accesed using pyrosequencing of the 16S rRNA V4 region. The bark of Euterpe edullis was higher at both sampling times (175,1±53,4 ng. cm-2. h-1, 97,2±21 ng. cm-2. h-1). The BNF rates were lower in soil. In high rainfall conditions, the BNF in the phyllosphere of Guapira opposite increased significantly (52,0±12 ng. cm-2. h-1) when compared with Euterpe edullis (3,6 ± 06 ng. cm-2. h-1) and Guapira opposite (7,5 ± 1,3 ng. cm-2. h-1) phyllosphere. The value of 15N was higher in the soil where the rates of FBN was lower. In the phyllosphere and bark, C/N was lower when BNF was higher. BNF in soil great spatial variation with areas of high activity. The 18.629 sequences obtained were grouped into 16.727 Operational Taxonomic Units (OTUs) distributed in 35 phyla. The main phyla Proteobacteria represented 38% of the OTUs and Acidobacteria 12% of the UTOs. The classes Alphaproteobacteria and Gammaproteobacteria were the most abundant in the three compartmens. Alphaproteobacteria, Betaproteobacteria and Gammaproteobacteria were the main potential N-fixers. The abundance of nitrogen-fixing Cyanobacteria in the phyllosphere and bark was low, indicating that others diazotrophics also colonize these environments and contribute with BNF.

19

Tufail, Muhammad Aammar. "Use of plant growth promoting endophytic bacteria to alleviate the effects of individual and combined abiotic stresses on plants as an innovative approach to discover new delivery strategies for bacterial bio-stimulants." Doctoral thesis, Università degli studi di Trento, 2021. http://hdl.handle.net/11572/305571.

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Bacterial endophytes are the organisms that live inside the plant for a full or a part of their life cycle. Endophytic bacteria have captured the interest of agriculture industry due to their plant beneficial properties, such as synthesis of phytohormones, solubilization of soil nutrients, and alleviation of biotic and abiotic stresses. Several studies have reported that stress tolerant endophytic bacteria can work with a similar performance as non-stressed conditions when inoculated to the plants under stressed conditions. Combination of abiotic stresses such as salinity, drought and low nitrogen stress can have additive or agonistic effects on bacterial and plant growth, and their interactions. However, very few studies have reported the impact of combined stress on endophytic bacterial assisted plant growth promotion. Therefore, understanding the underlying mechanisms of endophytic bacterial assisted plant’s tolerance abiotic stresses may provide the means of better exploiting the beneficial abilities of endophytic bacteria in agricultural production. Thus, the aim of this thesis was to study the stress tolerance mechanisms, beneficial characteristics, and plant growth promotion characteristics of endophytic bacteria under individual and combined abiotic stresses. Transcriptome analysis of endophytic bacteria revealed that tolerance mechanisms to deal with one kind of stress is different than concurrent stresses. Salinity and drought stress largely modulated the genes involved in flagellar assembly and membrane transport, showing reduced motility under stress conditions to preserve the energy. Additionally, bacterial endophyte that can fix nitrogen was studied with maize plant growth promotion under drought and low nitrogen stress conditions. The results suggested that diazotrophic bacterial endophyte can promote plant growth under moderate individual and combined stress conditions. Plant growth promoting endophytic bacteria can be utilized as an efficient tool to increase crop production under individual and concurrent abiotic stresses.

20

Tozer, Wade. "Nitrogen isotope variation in the environment : implications for interpretation /." 2006. http://adt.waikato.ac.nz/public/adt-uow20070124.124737/index.html.

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21

Ndiaye, Aissatou. "Impact of a red clover winter cover crop on carbon and nitrogen mineralization by microorganisms in soil aggregates." Thesis, 1998. http://hdl.handle.net/1957/33785.

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Although legumes have been widely studied for their nitrogen-fixing ability, it is uncertain to what extent legume cover crops achieve their nitrogen-fixing potential under the climatic conditions encountered in western Oregon. Furthermore, it is unknown what factors control the proportions of legume cover crop N that are either sequestered into soil organic matter, or that contribute to the N requirements of the following summer crop. Soil was sampled in mid-September 1997, after harvest of a summer broccoli crop, from plots located at the North Willamette Research and Extension Center, Aurora, Oregon. Soil was sampled from main plots that had been either winter cover cropped with red clover (LN�� and LN��) or fallowed during the winter period (FN�� and FN��), and specifically from sub-plots in which the following summer crop had received either zero (N��) or an intermediate (N��) rate of N fertilizer as urea. Levels of total organic carbon (TOC), total Kjeldahl nitrogen (TKN), and readily mineralizable C and N were measured in both whole soil samples and in different aggregate-size classes (<0.25, 0.25-0.5 0.5-1.0, 1.0-2.0, and 2-5mm) prepared by dry sieving the soil. Aggregate size-class distribution was not affected by the cover crop treatment. Although there was no significant effect of cover crop treatment on either TKN or TOC levels in whole soil samples, TOC levels were consistently higher in the small aggregate size-classes <1 mm of the fallow than the legume treatment. There was a significantly higher level of mineralizable C in the <0.25 mm size class of the legume than the fallow treatment. There was a trend for the level of mineralizable N to be greater in soil from the legume than the fallow treatment. However, N fertilizer had a significant positive effect on the level of readily mineralizable N in both fallow and legume cover-cropped treatments, it had a negative effect on TKN levels among all aggregate-size classes. There were differences in the levels of mineralizable N measured among the aggregate-size classes, and immobilization of N between 20 and 40 days of incubation also differed among the aggregate-size classes.
Graduation date: 1999

22

Kifle, Medhin Hadish. "Development of free-living diazotrophic (FLD) inoculants and their effects on crop growth." Thesis, 2008. http://hdl.handle.net/10413/5277.

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In this study several free-living diazotrophs (FLD) were isolated and screened for their nitrogen fixing ability on a range of crops grown in greenhouse, hydroponics and field trials. Rhizosphere isolates of free-living diazotrophs (FLD) may be effective biofertilizer inoculants, and may improve plant health where crops are grown with little or no fertilizer, as is the case in the Developing World. FLD isolates from rhizospheric soils in KwaZulu-Natal were assessed by growing them on N-free media, which is a key isolation method. They were then evaluated for their nitrogenase activity by quantifying ethylene production from acetylene by gas chromatography (GC). The free living isolates that produced greater quantities of ethylene were detected by an acetylene reduction assay (ARA). These were further assessed for colony formation on N-free media with different carbon sources, and at a range of temperatures (20, 25 and 300C) and pH values (6.0, 7.0 and 8.0). Isolates G3 and L1 were identified using DNA sequencing by Inqaba Biotechnical Industries (Pty) Ltd as Burkholderia ambifaria Coenye et al, and Bacillus cereus Frankland, respectively. These isolates grew significantly better on an ethanol medium, at temperatures of 20, 25 and 300C and pHs of 6.0, 7.0 and 8.0. Isolates B3 (Burkholderia sp.) and D6 (Bacillus cereus Frankland) also grew well on an ethanol medium, but only at 200C and at a pH of 6.0 and 7.0, respectively, while Isolate E9 (Burkholderia cepacia Frankland) grew well on an ethanol medium only at 300C, and pH 6.0 and 7.0. Temperature and pH strongly influence FLD growth on N-free media using different carbon sources. Further trials were conducted to screen the best isolates under greenhouse condition, using both seed treatments and drenching application techniques onto several crops. The drenching application resulted in an increase in the growth and N-total of all the evaluated crops, relative to an unfertilized control. Growth and N-total of maize and sorghum increased with seed treatments, but did not increase the growth of lettuce and zucchini. Drenching of FLD isolates at 106cfu ml-1, applied on weekly basis, resulted in an increase in the growth of lettuce. Increased doses and frequency of application of the FLD bacteria resulted in a decrease in lettuce growth. This led to the conclusion that application of FLD bacteria at high doses and short intervals may create a situation where the applied FLD bacteria and the resident rhizosphere microbes compete for root exudates. High doses at low frequencies and low doses at high frequencies may be more effective on lettuce. Inoculation of Isolate L1 (B. cereus) at 106cfu ml-1 or in combination with Eco-T® (Trichoderma harzianum Rifai), significantly increased growth of lettuce. This result may have been due to nitrogen fixation, or to secretion of growth promoting substances by both the FLD and T. harzianum, and to biocontrol effects of Eco-T®. Application of Isolate L1 (B. cereus) at 106cfu ml-1 with or without Eco-T® was an effective tool for enhancing plant growth and nitrogen fixation. An FLD, Isolate L1 (B. cereus), was applied to lettuce plants together with a complete hydroponics fertilizer at 25% strength (Ocean Agriculture 3:1:3 (38) Complete), with the N level at 25mg l-1. These plants grew significantly better than the control plants grown on 25% of normal NPK fertilization, or with an inoculation of L1 alone. This indicates that it may be possible to integrate FLD applications with the application of low levels of commercial fertilizers, which is what resource poor farmers can afford.
Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2008.

23

Lotfollahi, M. "The effect of subsoil mineral nitrogen on grain protein concentration of wheat / by Mohammad Lotfollahi." 1996. http://hdl.handle.net/2440/18896.

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Copy of author's previously published work inserted.
Bibliography: leaves 147-189.
xxi, 189 leaves : ill. (some col.) ; 30 cm.
Title page, contents and abstract only. The complete thesis in print form is available from the University Library.
This project examines the uptake of mineral N from the subsoil after anthesis and its effect on grain protein concentration (GPC) of wheat. The overall objective is to examine the importance of subsoil mineral N and to investigate the ability of wheat to take up N from the subsoil late in the season under different conditions of N supply and soil water availability. Greenhouse experiments investigate the importance of subsoil mineral N availability on GPC of wheat and the factors that contribute to the effective utilisation of N. The recovery of N from subsoil, the effect of split N application on GPC and short term N uptake by the wheat at different rooting densities are also studied.
Thesis (Ph.D.)--University of Adelaide, Dept. of Soil Science, 1997

24

Farr, Janet Dawn. "The performance of Uraba lugens Walker (Lepidoptera : nolidae) in relation to nitrogen and phenolics in its food." Thesis, 1985. http://hdl.handle.net/2440/20376.

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25

Farr, Janet Dawn. "The performance of `Uraba lugens` Walker (Lepidoptera : nolidae) in relation to nitrogen and phenolics in its food / by Janet Dawn Farr." 1985. http://hdl.handle.net/2440/20376.

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Bibliography: leaves 137-167
viii, 189 leaves, [31] leaves of plates : ill. (some col.) ; 30 cm.
Title page, contents and abstract only. The complete thesis in print form is available from the University Library.
Thesis (Ph.D.)--University of Adelaide, Dept. of Entomology, Waite Agricultural Research Institute, 1986

26

Keith, Heather. "Effects of fire and fertilization on nitrogen cycling and tree growth in a subalpine eucalypt forest." Phd thesis, 1991. http://hdl.handle.net/1885/142474.

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27

Rambau, Mashudu Daniel. "The effects of fertilization with bio-digester slurry and the inclusion of carbohydrate additives at ensiling on the nutritive value of Napier grass (pennisetum purpureum) silage." Diss., 2016. http://hdl.handle.net/11602/696.

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MSCAGR (Animal Science)
Department of Animal Science
The objective of the study was to determine the effects of fertilisation with bio-digester slurry and the inclusion of carbohydrate additives at ensiling on the fermentation characteristics, chemical composition, ruminal degradability, and in vitro digestibility of Napier grass silage. Napier grass planted at the School of Agriculture Experimental Farm, University of Venda in 5 m x 4 m plots replicated three times in a completely randomised design and was irrigated with either biodigester slurry or no bio-digester slurry (tap water) for a period of 12 weeks. After 12 weeks, the Napier was freshly cut and ensiled for 90 days in 1 litre glass jars in a 2 (Control - tap water and slurry irrigation) x 4 (No additive, molasses, maize meal and brown sugar) factorial arrangement. Fermentation quality and nutritive composition were determined using standard protocols. The dry matter (DM) and crude protein (CP) ruminal degradability was determined in sacco by incubating feed samples in nylon bags (external dimension: 6 × 12 cm, pore size of 46 μm) in the rumen in three Bonsmara steers fitted with rumen cannulae for 0, 6, 12, 24, 48, 72, 96 and 120 hours (h). Parameters to describe the dynamics of ruminal degradability of DM and CP were obtained by fitting the data on the exponential equation P = a + b (1 - e-ct) using the NEWAY computer program, where “a” is the rapidly degradable fraction, “b” is the slowly degradable fraction and “c” is the outflow rate. The in vitro DM and CP degradability of rumen undegradable residue collected after 12, 24 and 48 h incubation was determined by sequential digestion in pepsin (abomasal) and pancreatin (small intestine) solutions. Fertilisation with bio-digester slurry increased (P <0.05) CP content of fresh cut Napier grass pre-ensilage. Bio-digester slurry fertilisation with molasses inclusion improved (P <0.05) the silage DM content which improved (P >0.05) fermentation characteristics with pH of 4.2 and lowest NH3-N of 13.3 g/kg. Other chemical compositions and fermentation characteristics were not affected (P >0.05) due to fertilisation x additives treatment combinations. No bio-digester slurry fertilisation with maize meal inclusion increased (P <0.01) DM degradability at 0 h incubation. As time progressed to 24 h, no biodigester slurry fertilisation with no additive included reduced (P <0.01) DM degradability with no difference (P >0.05) on other treatments. Potential DM degradability (a + b) of no bio-digester slurry fertilisation with no additive inclusion silage was reduced (P <0.01). The reduction was associated with low levels (P <0.01) of slowly degradable fraction “b”. In vitro DM and CP digestibility were not affected (P >0.05) due to fertilisation x additives treatment combinations. In conclusion, bio-digester slurry application improved the quality of fresh cut Napier grass, with the combination of bio-digester slurry fertilisation and molasses addition yielding the best silage quality.