Journal articles on the topic 'Nick Mount'

In this workshop, Professors Nick Bontrager and Taylor Hokanson introduce the tools and services required to fabricate printed circuit boards, or PCBs. The process is more accessible than ever before, and is cheap enough to allow individuals to obtain functional prototypes, in small quantities, that are indistinguishable from similar PCBs that might be produced in the hundreds of thousands for industry or commercial applications. Using an “unpopulated” demonstration board designed by the workshop co-chairs, participants walk through all the steps needed to assemble and solder surface-mount components, finishing the event with a battery-operated, wearable, LED light display.

Embryonic suspensor in angiosperms is a short-lived structure that connects the embryo to surrounding maternal tissues, which is necessary for early embryogenesis. Timely degeneration via programed cell death is the most distinct feature of the suspensor during embryogenesis. Therefore, the molecular mechanism regulating suspensor cell death is worth in-depth study for embryonic development. However, this process can hardly be detected using conventional methods since early embryos are deeply embedded in the seed coats and inaccessible through traditional tissue section. Hence, it is necessary to develop a reliable protocol for terminal deoxynucleotidyl transferase (TdT) dUTP Nick-End Labeling (TUNEL) analysis using limited living early embryos. Here, we provide a detailed protocol for the whole-mount detection of suspensor cell death using a TUNEL system in tobacco. This method is especially useful for the direct and rapid detection of the spatial-temporal characters of programed cell death during embryogenesis, as well as for the diminishment of the artifacts during material treatment by traditional methods.

In investigating pathological changes that occur during development of fibrosis in lungs exposed to silica, we found a significant increase in apoptotic cells in rats exposed to silica by inhalation for 80 days. Since silica is not definitively detected at the light level, we sought to use SEM backscatter imaging to correlate the presence of silica with apoptosis identified by fluorescent assays. To accomplish this, paraffin sections of lungs from rats that had been exposed to silica were placed on carbon planchets. Serial sections were placed on glass slides, immediately before and after the section on the planchet. Using a fluorescein apoptosis detection system (Promega G3250), a TdT-mediated dUTP nick-end labeling (TUNEL) assay was performed on both planchets and slides. The planchets and slides were cover slipped with gel/mount (Biomedia) and viewed in an Olympus AZ70 fluorescent photomicroscope with a wide band blue filter cube. Apoptotic nuclei could be seen in similar regions in both the slide and the planchet preparations.

Cell death occurs during early development in vivo and in vitro, although little is known about the mechanism of blastomere death and the relation to embryonic loss. Apoptosis, characterised by chromatin condensation, DNA fragmentation and membrane blebbing, occurs without damage to surrounding cells in contrast to necrosis. Bovine oocytes and in vitro fertilised embryos (total n = 449) were analysed for (1) DNA fragmentation using terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling (TUNEL) and (2) morphological features of apoptosis. TUNEL labelling was detected in immature and mature oocytes (7%, n = 57 and 23%, n = 60, respectively), and at least one cell of 8- to 16-cell embryos (5%, n = 57), morulae/early blastocysts (79%, n = 39) and expanded/hatched blastocysts (100%, n = 48). In contrast, TUNEL labelling was not detected in zygotes (n = 61), 2-cell embryos (n = 46) or 3- to 7-cell embryos (n = 81). Chromatin condensation, nuclear fragmentation, absence of neighbouring cell destruction and extrusion of cells was frequent among advanced stage embryos. Although not detected during early cleavage under standard conditions, TUNEL labelling indicative of apoptosis was induced by treatment with 10 μM staurosporine for 30 h in 95% of cleavage stage embryos (n = 59). Determination of the expression and localisation of the p53 tumour suppressor gene using reverse transcription polymerase chain reaction and whole-mount immunofluorescence revealed that although p53 transcripts were present throughout early development, nuclear localisation of p53 protein could not be detected in any blastocyst suggesting p53-independent apoptosis. This study has shown that apoptosis is dependent on embryonic developmental stage after standard culture. This suggests that bovine embryos become more capable of accommodating damaged or abnormal cells as development proceeds.

Glutathione peroxidase 3 (GPx3) belongs to the glutathione peroxidase family of selenoproteins and is a key antioxidant enzyme in multicellular organisms against oxidative damage. Downregulation of GPx3 affects tumor progression and metastasis and is associated with liver and heart disease. However, the physiological significance of GPx3 in vertebrate embryonic development remains poorly understood. The current study aimed to investigate the functional roles of gpx3 during embryogenesis. To this end, we determined gpx3’s spatiotemporal expression using Xenopus laevis as a model organism. Using reverse transcription polymerase chain reaction (RT-PCR), we demonstrated the zygotic nature of this gene. Interestingly, the expression of gpx3 enhanced during the tailbud stage of development, and whole mount in situ hybridization (WISH) analysis revealed gpx3 localization in prospective tail region of developing embryo. gpx3 knockdown using antisense morpholino oligonucleotides (MOs) resulted in short post-anal tails, and these malformed tails were significantly rescued by glutathione peroxidase mimic ebselen. The gene expression analysis indicated that gpx3 knockdown significantly altered the expression of genes associated with Wnt, Notch, and bone morphogenetic protein (BMP) signaling pathways involved in tailbud development. Moreover, RNA sequencing identified that gpx3 plays a role in regulation of cell death in the developing embryo. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and phospho-histone 3 (PH3) staining confirmed the association of gpx3 knockdown with increased cell death and decreased cell proliferation in tail region of developing embryos, establishing the involvement of gpx3 in tailbud development by regulating the cell death. Furthermore, these findings are inter-related with increased reactive oxygen species (ROS) levels in gpx3 knockdown embryos, as measured by using a redox-sensitive fluorescent probe HyPer. Taken together, our results suggest that gpx3 plays a critical role in posterior embryonic development by regulating cell death and proliferation during vertebrate embryogenesis.

DURING A RECENT CONVERSATION WITH A HEAD NURSE, we started to reminisce about some of the units where we had worked. After thinking about our conversation for some time, I realized that I had the good fortune to have worked in the best NICU in the country. From 1980 to 1985, that was the NICU at Mount Zion Hospital in San Francisco.

Abstract Long-term survivors (LS) of metastatic colorectal cancer (mCRC) who experience multiple recurrences with resectable oligometastatic disease provide an opportunity to explore co-evolution of the tumor and immune microenvironment. We profiled 16 LS of mCRC with a median follow-up of 9.3 years and median of 3 biopsies/resections per patient (range 2-7). We performed multi-omic profiling of 56 primary and 176 metastatic samples from formalin-fixed paraffin-embedded tissue using low pass whole genome sequencing, 3’ RNA sequencing and DNA methylation arrays. A machine learning cell classifier was used to quantify immune cell and fibroblast infiltration from hematoxylin and eosin staining. Copy number profiling showed that the fraction of genome altered remained relatively stable across time and tissue type but that already-altered segments underwent progressive fragmentation. Inter-timepoint divergence of copy number alterations was significantly higher than intra-timepoint divergence, and intra-timepoint divergence was lower for liver and lung metastases than for primary tumors. Chemotherapy treatment did not significantly affect either divergence type. Differential expression and gene set enrichment analysis (GSEA) revealed common pathways dysregulated in metastases compared to primaries, including reductions in E2F (important in G1/S checkpoint) and G2M checkpoint, suggestive of the onset of senescence in metastases. Tumors underwent progressive hypomethylation over time and analysis of genes with concordant changes in promoter methylation and expression revealed dysregulation in pathways related to endocytosis, cell adhesion and migration. This suggests an important role for phenotypic plasticity in driving the metastatic phenotype. There were transient increases in the proportion of macrophages, lymphocytes and neutrophils in tumors that had undergone neoadjuvant chemotherapy in the 6 months prior to resection and slight increases in M1 macrophage activity (by GSEA) in tumors that were previously therapy naïve. There were concordant transient increases in pathways associated with immune response (MYC V1 and MTORC1), as well as xenobiotic metabolism. The latter is a known mechanism of drug resistance to both the platinum- and fluoropyridine-based therapies used in CRC. There were also more sustained increases post-chemotherapy in inflammatory and immune pathways associated with the adaptive immune response and tissue injury and repair. These findings were corroborated by concordant changes in promoter methylation. These data suggest that there is a threshold level of aneuploidy required to facilitate CRC metastasis but the migratory phenotype and adaptation to the metastatic niche are driven by plasticity. Chemotherapy induces differing short-term and long-term anti-tumor immune responses in the local microenvironment. We see evidence that mCRCs are able to mount plastic pro-survival mechanisms in response to chemotherapy. Citation Format: Alison May Berner, Calum Gabbutt, Salpie Nowinski, Jacob Househam, Nick Trahearn, George D. Cresswell, Vinaya Srirangam Nadhamuni, Christopher Kimberley, Matteo Fassan, Ann-Marie Baker, Andrea Sottoriva, Christina Thirlwell, John Bridgewater, Trevor Graham. Multiple roles for plasticity in metastasis and therapy resistance in long-term survivors of metastatic colorectal cancer [abstract]. In: Proceedings of the AACR Special Conference on the Evolutionary Dynamics in Carcinogenesis and Response to Therapy; 2022 Mar 14-17. Philadelphia (PA): AACR; Cancer Res 2022;82(10 Suppl):Abstract nr A045.

Few Capetonians would argue against the claim that the City has been rocked by the current water crisis that many have dubbed the most severe in modern history. Discussions about water saving techniques, membership of the ‘Water Warriors’ club, dinner party comparisons of family daily usage figures, discussion of toilet habits (to flush or not to flush?) and frenzied buying to secure 25-litre water containers have become part of daily life for those of us faced by the imminent (but previously unconscionable) threat of our taps running dry. Even the ‘proudly oily’1 premier of the Western Cape has boasted that she only showers every three days to help beat back Day Zero. But the water crisis has not only raised important questions about residents’ rights to, and responsibility for, the water they use. It has also brought to the surface interesting issues about criminality and crime control, and our individual and collective relationship to water. Stories of violence and incivility at water collection points and in supermarkets have captured attention on social media, and city dwellers have hotly debated the threat of organised crime, laws against rebottling and reselling of municipal water, and the Western Cape government’s Water Disaster Plan, which gives the police and army responsibility for maintaining safety and order at water collection points. Of course, while questions of water saving, risk and safety feel quite new to many Capetonians, scholars, activists and policymakers (including criminologists) have been writing about these issues for much longer. The Centre for Law and Society approached two scholars/activists to discuss the water crisis and its impact on questions of vulnerability, risk and security. Nick Simpson, an environmental and human development consultant (and post-doctoral scholar at the University of Cape Town), discussed questions of criminology in the age of the Anthropocene, and Vivienne Mentor-Lalu, a researcher/facilitator for the Women and Democracy Initiative at the Dullah Omar Institute at the University of the Western Cape, spoke to us about the gendered impact of the drought. Nolundi Luwaya, Kelley Moult, Diane Jefthas and Vitima Jere contributed to this piece.

Few Capetonians would argue against the claim that the City has been rocked by the current water crisis that many have dubbed the most severe in modern history. Discussions about water saving techniques, membership of the ‘Water Warriors’ club, dinner party comparisons of family daily usage figures, discussion of toilet habits (to flush or not to flush?) and frenzied buying to secure 25-litre water containers have become part of daily life for those of us faced by the imminent (but previously unconscionable) threat of our taps running dry. Even the ‘proudly oily’1 premier of the Western Cape has boasted that she only showers every three days to help beat back Day Zero. But the water crisis has not only raised important questions about residents’ rights to, and responsibility for, the water they use. It has also brought to the surface interesting issues about criminality and crime control, and our individual and collective relationship to water. Stories of violence and incivility at water collection points and in supermarkets have captured attention on social media, and city dwellers have hotly debated the threat of organised crime, laws against rebottling and reselling of municipal water, and the Western Cape government’s Water Disaster Plan, which gives the police and army responsibility for maintaining safety and order at water collection points. Of course, while questions of water saving, risk and safety feel quite new to many Capetonians, scholars, activists and policymakers (including criminologists) have been writing about these issues for much longer. The Centre for Law and Society approached two scholars/activists to discuss the water crisis and its impact on questions of vulnerability, risk and security. Nick Simpson, an environmental and human development consultant (and post-doctoral scholar at the University of Cape Town), discussed questions of criminology in the age of the Anthropocene, and Vivienne Mentor-Lalu, a researcher/facilitator for the Women and Democracy Initiative at the Dullah Omar Institute at the University of the Western Cape, spoke to us about the gendered impact of the drought. Nolundi Luwaya, Kelley Moult, Diane Jefthas and Vitima Jere contributed to this piece.

Abstract The Roquebrune-Cap Martin basin (RCMB), developed along the eastern rim of the Nice arc, represents an exclusive sedimentary marker constraining the timing of the deformation in the Nice arc (southern Subalpine chain) during Miocene times. Structural and sedimentological analyses as well as 3D geometrical modeling of the RCMB revealed morphological, structural and sedimentological features characterizing an active tectonic control of the sedimentary infills and the basin development. Structural and microstructural analyses along the eastern boundary of the Nice arc evidenced a N-S left-lateral strike-slip ‘en echelon’ faults system named Mont Gros-St Agnès Castillon relayed by the Biancon E-W thrusts and sheets. The formation of the RCMB appears to be genetically linked to these strike-slip ‘en échelon’ faults. Such characteristics include the presence of the Mont Gros strike-slip fault structural high relief bounding the RCMB to the West, the West-East asymmetry of the sedimentary infill with a laterally transition facies from breccias directly below the fault relief to conglomerates and sandstones in the central part of the basin and the presence of mass wasting in all structural levels of the basin. The onset and the evolution of the basin were driven by transpresssive tectonics, generating a deep and narrow tectonic depression, bounded by steep tectonically controlled slopes. The transpresssive character of the eastern Nice arc boundary where the syn-tectonic RCMB is hosted, accommodate a general southward translation of the Nice arc in response to a N-S shortening regime. The sedimentological and previous paleontological analyses suggest that the activity of the eastern Nice arc transpresssive boundary generating the RCMB and thus the southward motion of the Nice arc, started during the Early Miocene (Aquitanian), continuing through the Late Miocene (Tortonian). The style and the timing of the syn-sedimentary deformation of the Nice arc is coherent in space and time with the one affecting the Digne and Castellane arc.

On behalf of the Indonesian nation IR. Sukarno who was accompanied by Drs. Mohammad Hatta on August 17th, 1945 proclaimed Indonesian independence on Jalan Pegangsaan Timur No. 56 Jakarta at 10:00 am. News of the proclamation of Indonesian independence was only heard in Bali on August 23rd 1945 since the return of Mr. I Gusti Ketut Puja from Jakarta. News of the proclamation arrived late in Bali due to lack of communication and transportation with the island of Java. At this time Japan was still showing an attitude of power in Bali, therefore on December 13th 1945 a general attack was carried out against Japanese protests throughout Bali. This attack turned out to be a failure because Japan had known in advance. Since it was impossible to get weapons in Bali, I Gusti Ngurah Rai and his friends finally went to Java to report on the situation in Bali and asked for weapons. After some time living on the island of Java on April 4th, 1946 I Gusti Ngurah Rai and his friends finally returned to Bali where it landed at Yeh Kuning. From here I Gusti Ngurah Rai continued his journey to the village of Munduk Malang. From the village of Munduk Malang, I Gusti Ngurah Rai carried out an attack on the Dutch / NICA ambushes around him. Due to the position of I Gusti Ngurah Rai headquarters and its troops being known by the Netherlands / NICA, it was decided to hold a "Long March" or also known as "The June-July Trip". Long March starts by walking from Bengkel Anyar Village by climbing Mount Batukaru to Mount Agung. The purpose of holding a long march is to increase the people's enthusiasm, divert Dutch attention to the east, and facilitate assistance from Java to Bali. In addition to the fighters using walking during the physical revolution in Bali to be able to connect with other fighters also used transportation. Transportation is used by fighters for long trips and when the situation is safe. Transportation that is used at this time besides walking also uses such as truck, sedan, pickup, bus, bicycle, dock, cart, and boat for the sea. With this means of communication between the fighters remain connected so that the struggle in Bali can last long enough to face the Netherlands / NICA.

Hit & Run, by Nicky Hager and Jon Stephenson. Nelson: Potter and Burton. 2017, 159 pages. ISBN ISBN 978-0-947-50339-0.HIT & RUN tells what happened when a small group of New Zealand SAS soldiers in Afghanistan decided to mount a revenge raid on two Afghan villages where they thought Taliban they believed had been responsible for the death of one of their own were staying. With Ministerial approval, relying on faulty intelligence and backed by American firepower, the SAS raided the villages of Naik and Khak Kuday Dad on the night of 22 August 2010. Their Taliban targets were nowhere to be found: They had slipped away into the mountains and were resting peacefully when the SAS arrived by helicopter in the middle of the night.

The purpose of this study is to isolate, identify and specification of various the Candida species from various clinical samples in a tertiary care hospital, and to characterize various the isolated Candida species. A study was conducted on people of different age groups from January 2019 to December 2019. Candida species isolated from different patients by using Potassium Hydroxide mount and processed by BacTalert 3D (Biomerieux) automated blood culture system. Further culture identification of Candida species were done on Sabouraud dextrose agar (SDA). Speciation of Candida was done using Germ tube test, CHROM agar Candida Medium, Cornmeal agar, Sugar Fermentation test and Sugar Assimilation test.In our study was the most common species isolated, among non albicans Candida i.e. 21 (38.9%); 19(35.2%) of was the most common followed by 9(16.7%) ofand 5(9.3%) of . Maximum number of Candida isolates were obtained from NICU i.e. 27(50.0%) followed by 11 from Med (20.3%), 7 from E/W (13.0%), 2 from BICU (3.7%), 2 from Skin (3.7%), 1 from PICU (1.9%), and 1 from R/R (1.9%).Our study showed that is the most common isolates species. Among , was found to be the most common isolate followed by . Children less than 1 year are most affected with maximum number of Candida species were obtained from NICU department. HiChrom Candida is proven to be more useful as differential agar.

Different phase compositions and microstructures of oxide scales were formed on the surface of SS400 hot rolled alloys by employing various heat treatment processes. Cyclic wet-dry immersion corrosion test, electrochemical impedance spectroscopy were used to investigate the corrosion resistance of strips with scales fabricated by different heat treatment processes. The results reveal that difference in the corrosion resistance of the various scales is due to the difference in the grain size of Fe3O4phase. Furthermore, the difference in the corrosion resistance of different oxide phases, exhibited by various scales, also render the strips to give various corrosion behaviors. It is surmised that the strip with oxide scale, which consist of a small mount of the outer layer Fe2O3phase distributed continuously and a large quantity of the inner layer Fe3O4phase with the fine grain size, and possess nice compactness, continuity, integrity in the morphology structure, has the best corrosion resistance.

This research observed the Toya Devasya, Kintamani. The aim of this research are 1) to know the motivation of domestic tourist’s that visit the Toya Devasya Kintamani, Bangli. 2) to know their perception in regards of its service quality. This research used sample determination method which tend to purposive with taking its sample by using accidental sampling method and determining the number of samples using quota sampling giving 100 questioners to domestic tourist. Data collection technique that used is by interview, observing, documentation, literature study and questioner with analysis technique by qualitative and quantitative descriptive. The result showed the motivation which push domestic tourist’s to visit Toya Devasya are to make the mind calm, relax and free yourself in a beautiful and interesting place, get a new atmosphere. At the same time the motivation that pull those tourist’s to visit Toya Devasya is the beauty of natural mountain kicking, a nice atmosphere to be enjoyed, the source of hot water coming from Mount Batur. The perception of domestic tourist’s to its service quality over all is good, however they still feel that the lack of assurance service in Toya Devasya.

Daerah Istimewa Yogyakarta adalah provinsi yang rawan bencana terutama Gunung Merapi. Untuk meminimalkan korban bencana dikembangkan program kesiapsiagaan masyarakat menghadapi bencana gunung merapi melalui desa siaga. Tujuan penelitian ini mengetahui pelaksanaan kegiatandan penerapan program kesiapsiagaan masyarakat menghadapi bencana gunung merapi di Desa Siaga Umbulharjo. Penelitian ini menggunakan rancanganpenelitian studi kasus dengan analisis kualitatif. Subjek yang diteliti adalah pelaku pelaksanaan kegiatan pada pelaksanaan desa siaga, sedangkan objek penelitian adalah situasi sosial dalam pelaksanaan kegiatan. Pengumpulan data dilakukan dengan metode observasi nonpartisipatif, wawancara mendalam, dokumentasi, member check, dan triangulasi.Pelaksanaan kegiatan sudah baik, pembiayaan sudah cukup, pelatihan-pelatihan sudah baik, sosialisasi, sarana-sarana yang ada, kinerja kader, dan peran Puskesmas baik. Namun, keberhasilan pelaksanaan kegiatan kesiapsiagaan tersebut belum diikuti dengan keberhasilan pelaksanaan desasiaga. Masih banyak masyarakat yang tidak mengetahui tentang desa siaga. Pelaksanaan kegiatan kesiapsiagaan sudah baik, tetapi tidak diikuti dengan pelaksanaan desa siaga secara umum.Kata kunci: Kesiapsiagaan, bencana gunung merapi, desa siagaAbstractYogyakarta Province is a disaster-prone province, especially the mountain trim. Disaster preparedness is essential to minimize the disaster victims so formed the community program on disaster preparedness through Desa Siaga of Mount Merapi alert. To determine the activity and how the implementationof community disaster preparedness programs Mount Merapi onthe implementation of Desa Siaga in the Umbulharjo Village alert This study was a descriptive study using a case study research design with qualitative analysis. Subjects in the study was the perpetrator of the community in dealing with disaster preparedness merapi mountain on implementation of desa siaga with the object of research is the social situation in the implementation of preparedness merapi mountain. Resource persons determined by the method of purposive and snowball effect if necessary. The collection of data by way of nonparticipatory observation, indepth interviews, documentation, member check, and triangulation. Implementation of the activities of disaster preparedness in the face of the mountain communities have nice views of the trim is already sufficient financing, training, training has been good from the parties involved, socialization, existing facilities, the performance of cadres, and the role of good health. The success of the implementation of disaster preparedness in the face of the mountain communities are not followed by smoothing the successful implementation of the desasiaga, seen from many people who do not know what a desa siaga. Implementation of the activities of disaster preparedness in the face of the mountain communities have a good trim but it is not followed by the implementationof desa siaga in general.Key words: Preparedness, mount merapi disaster, desa siaga

Abstract Background Inflammatory bowel disease (IBD) disease activity during pregnancy is related to adverse neonatal and pregnancy-related outcomes. Biologics are used to suppress disease activity, however, since there is known transplacental passage, the American Gastroenterology Association (AGA) recommends timing the final dose with drug-specific half-lives although there is little evidence demonstrating adverse outcomes. Aims We aim to assess the safety of early versus late discontinuation of biologics according to drug-specific half-lives by comparing various neonatal and pregnancy-related outcomes. Methods This is a REB approved single-center retrospective cohort study on all patients with IBD ≥18 years of age on a biologic agent prior to conception, have a documented final dose during pregnancy, and were seen at Mount Sinai Hospital from 2016–2019. Neonate and pregnancy-related outcomes were compared amongst the two groups (Table 1) using the student’s t-test (birthweight, gestational age, Apgar scores) and Fischer’s exact test (NICU admission, congenital anomalies, GBS, chorioamnionitis) analyzed in SPSS Version 27. The level of significance was set at p<0.05. Results We identified 53 patients on biologics pre-conception. 26 patients had a documented final dose (19 early cohort, 7 late cohort) and were included in the analysis. Aside from mean birthweight (3014 vs 3561 g, p=0.036), there were no statistically significant differences between the early and late cohorts for gestational age (37.4 vs 39.0 weeks, p=0.20), 1- and 5-min Apgar scores (7.8 vs 8.8, p=0.37 and 8.5 vs 9.0, p=0.49), NICU admissions (p=0.54), congenital anomalies (p=1.00), GBS (p=0.55), and chorioamnionitis (p=1.00). Conclusions Overall, our study suggests that early and late discontinuation of biologics have comparable safety profiles based on various neonatal and pregnancy-related outcomes. In fact, we see significantly higher birthweights in the late cohort along with a consistent (non-statistically significant) trend of later gestational ages, and higher Apgar scores. Further, no cases involving NICU admissions, congenital abnormalities, GBS, or chorioamnionitis were seen in the late cohort. Next, we hope to verify our findings by conducting a prospective cohort study with a larger study population and more comprehensive data collection. This will provide higher statistical power and allow for additional subgroup analyses based on objective disease activity (FCP levels) and therapeutic drug monitoring (serum drug levels). Funding Agencies None

Abstract Background Inflammatory bowel disease (IBD) disease activity during pregnancy is associated with adverse neonatal and pregnancy-related outcomes. Biologics are used to suppress disease activity but crosses the placenta in the third trimester. Conflicting studies and guidelines on the timing of biologic dosing in pregnancy persist as we try to balance the risk of disease flare and possible adverse drug effects. The American Gastroenterology Association (AGA) recommends timing the final dose according to the half-life and dosing regimen of each biologic agent. Aims To compare neonatal and pregnancy-related outcomes in early versus late dosing of biologics. Methods This was a single-center retrospective cohort study conducted at Mount Sinai Hospital from 2016–2021. We included patients with an established diagnosis of IBD before pregnancy who were at least 18 years of age at the time of conception. All patients must have been treated with an IBD-specific biologic agent and had a documented final dose during the pregnancy. The early group received their last biologic dose earlier than the AGA recommendations and the late group received it within the recommended interval. A patient was considered to have a flare based on the overall clinical impression of their gastroenterologist informed by reported symptoms, investigations (fecal calprotectin, endoscopy), and response to treatment. Neonatal and pregnancy-related outcomes were compared amongst the two groups using the student’s t-test (for continuous variables) and Fischer’s exact test (for categorical variables) using SPSS Version 27. Results Of 322 patients who had a completed pregnancy at Mount Sinai Hospital, 107 were included in this study. 67 (62.6%) were in the early and 40 (37.4%) were in the late groups. Baseline characteristics including age, comorbidities, IBD phenotype and disease activity were similar between the two groups. The late group had significantly later gestational ages (37.4 vs 38.7 weeks, p=0.006), higher 5-minute Apgar scores (8.7 vs. 9.0, p=0.042), fewer NICU admissions (25.4% vs 5.0%, p=0.036), and fewer IBD flares (28.3% vs 11.1%, p=0.039) in the 6-month post-partum period. There were no significant differences in the rates of premature birth, caesarian sections, infections, and congenital abnormalities. Results are displayed in figure 1. Conclusions Our study suggests that late dosing of biologics according to the AGA guidelines was associated with favourable outcomes. However, this is an unadjusted analysis based on retrospective data and findings should be confirmed in a prospective manner to account for confounders. Funding Agencies None

In his provocative polemic“The New Hedonism,” Grant Allen mounts a passionate defense offin-de-siècleaestheticism by proposing a modern ethic – the titular “new hedonism,” which he borrows from Oscar Wilde's novelThe Picture of Dorian Gray(1890, rev. 1891) – that fully synthesizes aestheticism's insights with up-to-date scientific knowledge. At first glance, Allen seems an unexpected ally for Wilde, in part because few literary historians have explored the link between the two contemporaries. Many modern-day scholars of Allen's work (including Peter Morton, Bernard Lightman, William Greenslade, and Terence Rodgers) have tended to focus on his popular science writing, his elaborations on Herbert Spencer's evolutionary theories, and his controversial “New Woman” novelsThe Woman Who Did(1895) andThe Type-Writer Girl(1897). Those who do connect Allen and Wilde, such as Nick Freeman, often draw the relationship into focus through the two writers’ shared interest in libertarian socialism rather than their overlapping philosophical and aesthetic concerns (111–28). Yet, as we can begin to see in the epigraphs, the association that Allen made between evolutionary progress and the “beautifying of life” echoes one of the most significant claims of Wilde's earlier, dialogic essay, “The Critic as Artist.” “Aesthetics,” Wilde's speaker, Gilbert, enthuses, “like sexual selection, make life lovely and wonderful, fill it with new forms, and give it progress, and variety and change” (“The Critic as Artist” 204). Allen's survey of human evolutionary history reminds him, too, that our cultural and artistic achievements are all that lift us “above the beasts that perish” (382). For both writers, then, aestheticism's commitment to beauty, “self-development,” and the emancipated pursuit of pleasure entails potentially sweeping consequences for the future evolution of humankind. Allen's vocal support for Wilde – which Allen expressed privately in letters as well as publicly in his 1891 article “The Celt in English Art” – was not simply a convenient political alliance, but an integral part of Allen's complete program for sociocultural improvement.

The Australian and Papua New Guinean species of the water beetle genus Gymnochthebius Orchymont, 1943, are revised, based on the study of 4,904 specimens. The genus is redescribed, and redescriptions are provided for G. australis (Blackburn), G. brisbanensis (Blackburn), G. clarki (Deane), G. levis (Deane), G. lividus (Deane), G. notalis (Deane), and G. tenebricosus (Deane). Lectotypes are designated for Ochthebius australis Blackburn, 1888, and Ochthebius tenebricosus Deane, 1931. Ochthebius fischeri Deane, 1931, and Ochthebius leai Deane, 1931, are synonymized with Ochthebius australis Blackburn, 1888; Ochthebius flavocinctus Deane 1933, is synonymized with Ochthebius lividus Deane, 1933; and Ochthebius angustipennis Deane, 1931, is synonymized with Ochthebius clarki Deane, 1931. Twenty-nine new species are described, and a key to the 36 species known from Australia and Papua New Guinea is given. High resolution digital images of all primary types are presented (online version in color), the male genitalia are illustrated, and Australian geographic distributions are mapped. Only one species, G. clarki, inhabits both Australia and Papua New Guinea; two species, G. bacchusi n. sp. and G. papua n. sp. are endemic to Papua New Guinea; 33 species are endemic to Australia. Members of Gymnochthebius are found at the gravelly/sandy/silty margins of flowing and standing water. A preliminary grouping of species according to microhabitat substrate is presented. Correspondences between ventral morphology and microhabitat preferences suggest that a few species are evolving toward humicolous habits. New species of Gymnochthebius are: G. angulonotus (Queensland, Tinaroo Creek Road via Mareeba), G. bacchusi (Papua New Guinea, Morobe District, c. 7 miles Lae Bulolo Road), G. benesculptus (South Australia, Warburton River, 1 km N White Bull Yard Kalamurina Stn.), G. coruscus (South Australia, Warburton River, 1 km N White Bull Yard Kalamurina Stn.), G. fontinalis (South Australia, Elizabeth (Mound) Springs, 7 km NW Coward Springs R.S.), G. fumosus (New South Wales, Sydney), G. hesperius (Western Australia, Lyndon River Bridge), G. inlineatus (Western Australia, Millstream, creek near Deep Reach), G. lustrosulcus (Queensland, Cloncurry), G. minipunctus (Northern Territory, Palm Valley), G. nanosetus (Northern Territory, Roderick Creek, Gregory National Park), G. nicki (Victoria, Possum Hollow falls, West branch Tarwin River, 5.6 km SSW Allambee), G. nigriceps (South Australia, Mound Spring near Coward Springs), G. papua (Papua New Guinea, Morobe District, ca. 10 km S Garaina Saureri), G. perpunctus (South Australia, Somme Creek, between Angaston and Sedan), G. pluvipennis (South Australia, Warburton

Mitos Sangkuriang merupakan mitos yang pesan kearifan tradisinya “gagal” dicerna oleh masyarakat pemiliknya. Mitos ini pada akhirnya hanya mampu menceritakan asal mula ”kelahiran” sebuah gunung dan cekungan Bandung, dan tidak ada hubungannya dengan kehidupan manusia kecuali tentang perjalanan kehidupan seorang anak manusia yang durhaka pada orang tuanya. Tampak bahwa, pesan-pesan mitos ini sedemikian naifnya jika dibandingkan dengan dinamika perjalanan kehidupan yang dijalani oleh manusia Sangkuriang. Karena itulah perlu cara pandang, pemahaman dan penafsiran yang tidak hanya menangkap berbagai fenomena atas peristiwa yang terjadi dalam kehidupan sehari-hari tetapi juga untuk melihat ‘batin sosial’ suatu masyarakat bahkan melihat ke dalam ‘struktur dalam’ (deep structure) suatu masyarakat. Dengan demikian mitos sebagai sebuah ”proses komunikasi” lintas generasi dalam tataran simbolis mampu hadir dan bermakna dalam tatanan yang apik dan rapih sebagai alternatif solusi mengatasi atau memecahkan berbagai kontradiksi empiris yang selama ini tidak terpahami oleh nalar manusia. Abstract: Sangkuriang myth represents myth whose message of its tradition wisdom is "fail" to be digested by its owner society. This myth in the end only can narrate provenance "birth" a Bandung hollow and mount, and there no relation with human life except about life journey a disaffected human being child at its old fellow. See that, messages of this myth is naive in such a way in comparison with life journey dynamics experienced by Sangkuriang human being. Therefore, it needs a way of approach, interpretation and understanding which do not only catch various phenomenon to the event that happened in everyday life, but also to see 'social mind' society, even see into 'structure in' (structure deep) society. Thereby myth as a "communications process" passed by quickly generation in symbolic devices can attend and have a meaning of in nice structure and good alternatively solution overcome or solve various empirical contradiction is not comprehended by human being natural existence Key Words: incest, social veneering, and Sangkuriang

AbstractBackgroundPropofol can have adverse effects on developing neurons, leading to cognitive disorders, but the mechanism of such an effect remains elusive. Here, we aimed to investigate the effect of propofol on neuronal development in zebrafish and to identify the molecular mechanism(s) involved in this pathway.MethodsThe effect of propofol on neuronal development was demonstrated by a series of in vitro and in vivo experiments. mRNA injections, whole-mount in situ hybridization and immunohistochemistry, quantitative real-time polymerase chain reaction, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling, 5-ethynyl-2′-deoxyuridine labeling, co-immunoprecipitation, and acyl–biotin exchange labeling were used to identify the potential mechanisms of propofol-mediated zisp expression and determine its effect on the specification of retinal cell types.ResultsPropofol impaired the specification of retinal cell types, thereby inhibiting neuronal and glial cell formation in retinas, mainly through the inhibition of Zisp expression. Furthermore, Zisp promoted the stabilization and secretion of a soluble form of the membrane-associated protein Noggin-1, a specific palmitoylation substrate.ConclusionsPropofol caused a severe phenotype during neuronal development in zebrafish. Our findings established a direct link between an anesthetic agent and protein palmitoylation in the regulation of neuronal development. This could be used to investigate the mechanisms via which the improper use of propofol might result in neuronal defects.

Mount Gongga is the highest peak in the Hengduan Mountain region and is located at the southeastern fringe of the Tibetan Plateau, Sichuan Province, southwest China. As a transitional zone between the Tibetan Plateau and Sichuan Basin, Gongga Mountain features particularly diverse topography, geology, climate, and biodiversity and is a globally significant hot spot of biodiversity.

The land of PT. Hide Way Resort area 1.5 ha located in Ngawas village, Pasuruan district, the Province of East Java as at the moment is empty land and was planted apple, Eugenia, red pepper, carrot and pumpkin. It is located almost at the same position with Mount Bromo. This land will be used for accommodation/ 10 villas and 1 meeting room. We have to drive and reach this location and can see the apple and vegetable garden on mountain also very nice scenery we can see during passing this location. Also, we can see the natural village condition and warmest welcome from the people around the village. We can see very nice view after reaching the place by mountain view and hill view in cold temperature. Land and hill view on the area make more convenience to stay here. This kind of location can attract the tourist especially specific tourist who always searching the specific destination since it is not many kind of destination like this. The main concept is staying in natural village with traditional villa style and look like the traditional house in the past including the furniture inside and how to cook the food by burning wood. This kind of situation also completed with daily people around the village activities, traditional art show, transportation to Mount Bromo, golf and other activities. The marketing activities will be used the relationship of the owner with his channels abroad, community of specific tourism, you tube, social media and online travel agent. This opportunity is good to be developed in the future since many inquiry for this kind of specific tourism, increase the economy level of the people around and create more working opportunity.

IntroductionPrimary amoebic meningoencephalitis (PAM) caused by Naegleria fowleri is seldom reported in mainland China.MethodsOne case from South China was presented, and the clinical features of the PAM, especially the early CT features, were compared to those in the literatures from PubMed/Web of Science/China National Knowledge internet (CNKI).Case Presentation and ResultsA 47-year-old man with a high fever came to the fever clinic. Twelve hours later, the man lost consciousness and exhibited generalized tonic-clonic seizures and needed ventilator-controlled ventilation. Then, he was admitted to the neurology intensive care unit (NICU). The opening pressure of his cerebrospinal fluid (CSF) was over 500 mm H2O with highly increased leukocyte/protein levels and very low glucose levels. Three days after admission, high copy numbers of Naegleria fowleri amoebae were detected by metagenomics next-generation sequencing (mNGS) and cysts were visible with wet mount microscopy. Four days after admission, the patient experienced brain death. However, the relatives of the patient did not want to give up, and he received amphotericin B (AmB). During hospitalization, he suffered from severe damage to the liver and kidneys and electrolyte disorders that required continuous renal replacement therapy (CRRT).ReviewAll 20 included PAM patients suffered from fever. Seventeen of them had headache and neck stiffness. Ten of them showed generalized brain edema. To date, 7 cases of PAM have been reported in China. Only one patient survived. Most of the patients showed generalized brain edema. Only the surviving patient showed focal edema. He died three months later.ConclusionRapidly progressive meningoencephalitis in which the CSF results are similar to those suffered from a bacterial infection should be considered a possible case of PAM. It can be rapidly detected with microscopy in CSF wet mounts but needs further molecular investigation for confirmation, and mNGS should be a new method used for rapid and precise identification. Moreover, CRRT may prolong the survival time of PAM patients with multiple organ failure.

ApoA-1 binding protein (AIBP), a mediator of cholesterol efflux from endothelia cells to HDL, plays a vital role in zebrafish angiogenesis. Although it is evolutionary conserved from Drosophila to human, its role of angiogenesis in higher vertebrate has not been studied. Thus, we propose that the role of AIBP in angiogenesis is also conserved from zebrafish to mice to humans. Mouse models display tremendous power in the study of human cardiovascular disease as the murine and human vasculatures are highly similar. We therefore generated Aibp -/- mice, which are viable and fertile. We then compared retinal vasculature development in Aibp -/- mice and wild-type control mice by whole mount immunostaining. Our data show that AIBP deficiency in mice results in significantly enhanced angiogenesis, including an increase in the radial extension of vascular plexus, the development of a denser upper capillary layer, and more tip cells as well as filopodia in the retinas. Notch signaling pathway plays an essential role for proper angiogenesis. However, the role of cholesterol metabolism in the Notch pathway is poorly understood. We found the expressions of the Notch downstream targets Hes1, Hey1 and Hey2 were upregulated in Aibp -/- retinas compared to controls. Furthermore, the protein level of Notch intracellular domain (NICD) was decreased in Aibp -/- retinas. These results suggest that the activity of Notch signaling is decreased in retinas from Aibp -/- mice. Mechanistically, we found AIBP positively regulates Notch signaling by affect on γ-secretase activity. Moreover, we performed Matrigel plug assay. Comparing that of wild-type, Matrigel plugs from Aibp -/- mice has more VE-cadherin staining and higher VE-cadherin mRNA expression level; and shows lower Hey1 expression as well as decreased NICD protein levels. It indicates that Matrigel plugs from Aibp -/- mice expresses more blood vessels and show lower Notch signaling activity. Our study demonstrated that AIBP inhibits angiogenesis by activating the Notch pathway and are the first to connect AIBP, a cholesterol metabolism regulator, to Notch signaling.

Daerah penelitian dengan latar belakang Gunung Rinjani yang indah, memiliki potensi untuk dikembangkan sebagai daerah tujuan wisata pantai seperti Karang Bedil, Sesait, Desa Salangan, Sukadana, Tanjung Batu, Sungian, Labuhan Pandan, serta pantai timur Pulau Gili Sulat dan Gili Lawang. Untuk kegiatan wisata snorkling, diving dan surfing tempat yang baik adalah di Tanjung Awar-awar, Tanjung Menangis dan Tanjung Batu. Olah raga surfing (selancar air) disarankan sebaiknya dilakukan antara Bulan Oktober – Mei. Dalam membangun sarana penunjang di kawasan wisata pantai dan bahari faktor yang penting adalah memberikan perhatian khusus dalam menjaga kelestarian lingkungan daerah tujuan wisata, agar keaslian alam yang ditawarkan kepada wisatawan tetap asli. Kata kunci: Karakteristik pantai, sebaran sedimen permukaan dasar laut, batimetri, dan perubahan garis pantai. The study area, with the background of the beautiful Mount Rinjani, has a potency to develop as a coastal tourism such as Karang Bedil, Sesait, Desa Salangan, Sukadana, Tanjung Batu, Sungian, Labuhan Pandan, and the east coast of Gili Sulat and Gili Lawang Islands. For snorkling, diving and surfing the nice place is in Tanjung Awar-awar, Tanjung Menangis and Tanjung Batu. The right time for snorkling sport should be conducted between October - May. To build the supporting facilities in the coastal and venerable tourism objects, the important factor is to pay a special attention for keeping the everlasting environment of the tourism object destination, so that the original of nature which is offering to the tourists is still pristine. Keywords: coastal characteristic, sea floor surficial sediment, bathymetry and coastline chnges

Abstract Background Numerous laboratory and fewer field-based studies have found that ixodid ticks develop more quickly and survive better at temperatures between 18 °C and 26 °C and relative humidity (RH) between 75 and 94%. Ixodes anatis Chilton, 1904, is an endophilic, nidicolous species endemic to North Island brown kiwi (Apteryx mantelli) (NIBK) and the tokoeka (Apteryx australis), and little is known about the environmental conditions required for its development. The aims of this study were to determine and compare the conditions of temperature and RH that ensure the best survival of the kiwi tick and the shortest interstadial periods, in laboratory conditions and outdoors inside artificial kiwi burrows. Methods Free-walking engorged ticks were collected off wild kiwi hosts and placed in the laboratory under various fixed temperature and humidity regimes. In addition, sets of the collected ticks at different developmental stages were placed in artificial kiwi burrows. In both settings, we recorded the times taken for the ticks to moult to the next stage. Results Larvae and nymphs both showed optimum development at between 10 °C and 20 °C, which is lower than the optimum temperature for development in many other species of ixodid ticks. However, larvae moulted quicker and survived better when saturation deficits were < 1–2 mmHg (RH > 94%); in comparison, the optimum saturation deficits for nymph development were 1–10 mmHg. Conclusions Our results suggest that the kiwi tick has adapted to the stable, but relatively cool and humid conditions in kiwi burrows, reflecting the evolutionary consequences of its association with the kiwi.

"Birds of a feather (and colour) will flock (and fly) together." — Old English Proverb, 1545 (approx) While the notion of the 'coalition' is one normally associated with formalised alliances between political parties, coalitional affiliations are not limited to mainstream politics, and instead share a focus on strategy and outcome across the full range of human endeavours. Parties with varying priorities will put to one side their differences in order to focus on overlapping concerns. Thus coalitions come in all shapes and sizes and cross all walks of life: from families, clubs and teams to friendships, churches and sects, from companies and co-operatives to scientific formula, mathematical groupings and multimedia/multi person online gaming environments. This issue of M/C Journal mounts a timely critical reflection on the multiple contemporary meanings and uses of 'coalition' and coalitional thinking. Some of the questions the authors of this edition have addressed include: how does the notion of coalition inform political practices and powers? How have coalitions changed in recent times? What other (non-political party) coalitions exist and how might they work? How do coalitions inform understandings and expressions of race and whiteness, gender and sexuality, class and poverty, nations and borders? What does it mean to be 'post-coalitional' and how might we map persistence and change in recent political and non-political groupings and collectives? Recent history has revealed large cracks and major shifts in public and political alliances. In Australia for example, November 2007 marked a change in politics and culture that saw the demise of then Prime Minister John Howard and his Coalition government. The coupling of neoliberalism and social conservatism was said to be the hallmark of that government's commitment to 'old Australian values', to severe forms of border control, the refusal of same-sex marriage, scepticism toward climate change, and rapid privatisation policies for public services. The Coalition, it appeared, no longer represented the interests of the public. Since then, the incumbent Labor leader was deposed from within his own party, and Australia’s first female Prime Minister, after having lost a majority, formed a new coalition with smaller parties and independents in order to keep governing. This new coalition came in the wake of Britain’s 2010 election, in which the Conservatives joined with the Liberal Democrats to form the first ever British coalition government, and later was followed by the mid-term resurgence of Republican power in the American Lower House. And of course it was not too long ago that the ‘coalition of the willing', as a collective American-led force fighting the war-or-terror, fell apart in the later stages of the Bush administration, and that the 2008 shift in American politics to Barack Obama's presidency became a singular moment of international historical significance. We ask then, as connections to particular coalitions shift, what new affiliations are formed? And which aspects of older coalitions continue in the midst of change? What do regions, nations and individuals do when the groups they belong to fall apart or lose power? Larger coalitional shifts tell us much about culture, history, law, media, technology and human behaviour. As Australia and the Western world continually move away from supporting the power and policy of previously dominant groups, questions emerge as to the nature and ethics of collectives (of all kinds) as the expression of political, social and personal change. And despite these changes, borders are strengthened, and the associated fears of difference and otherness (from racism to war, Islamaphobia to homophobia) continue to reassert themselves across the globe. This logic of coalition is systematically unpacked and interrogated in this edition’s feature essay by Nick Mansfield. In “Coalition: The Politics of Decision”, Mansfield draws upon the work of Jacques Derrida to carefully analyse the implications of coalition for contemporary politics. Coalition can be distinguished from community or family, and is more akin to friendship, according to Mansfield. This is for two fundamental reasons: firstly, coalitions involve “decision”, and secondly, coalitions are always inevitably in relation to enmity. While coalitions start with a gesture of friendship, Mansfield argues via Derrida’s reading of Schmitt, that this category can slip into enemy territory. Mansfield’s paper uses this theoretical framework in order to comment on political action today. Indeed, each of the papers presented in this volume understand and illuminate ‘coalition’ as a critical tool or useful conceptual framework. In this collection, coalition is deployed in a manner which illuminates the relationships between different parties, interest groups, affiliational thinking and behaviours, and even the bodily senses. Coalitions are understood as contesting and reiterating dominant political paradigms and socio-cultural norms. Ann Deslandes, Randall Livingstone and Christopher Phillips bring our attention to the coalitions that contest dominant forms of political power. Deslandes’s thoughtful engagement with the ‘global justice movement’ focuses on the “ethical scene of activist coalition”. Deslandes examines what she terms the three ethics of coalition, risk, prayer and gift. In so doing, she asks important questions of privileged activists, who must risk the possibility of repeating “domination”. In both Livingston and Phillips, the Internet is a primary tool and site for critical engagement. Livingstone’s paper looks at the “virtual coalition” of online editors concerned with combating Western bias on the major Web encyclopaedia Wikipedia: “WikiProject: Countering Systemic Bias”. Since its inception in 2001, Wikipedia has grown in popularity to be one of the most accessed websites available. This research provides much needed insight into the extent to which Western bias frames the information uploaded to Wiki. At a time when there is a temptation to regard the Internet as liberatory, discussion of its uneven political power is significant. After all, as Livingstone reiterates, the Web does not dissolve border and boundaries. Further exploring relations between visibility and democracy. Phillips takes Gailbraith’s A Good Society as a starting point for a discussion of how contemporary coalitions work, and what larger coalitions of previously marginalised or silenced groups might look like. In this way, Phillips asks if Gailbraith’s vision has been borne out in the American context with particular reference to the 2008 Obama campaign’s use of Internet technologies and the more recent rise of the Tea Party to a position of considerable influence. Given the surprising similarities between such opposing forces, might an understanding of the coalitional ground shared by both be possible? An ongoing theme of this edition of M/C is its engagement with the current local/global coalitional and post-coalitional conditions in which people live - from larger contexts of geopolitics through to the micropolitics of everyday practices, pleasures and identifications. Elaine Kelly engages with the changes to land rights legislation in Australia over the past five years, with the Northern Territory Intervention and more recently with the decision by the Labor Party to uphold the nomination of Muckaty as a site for nuclear waste. Kelly extends the discussion of coalition to encompass its etymology – to grow together. Framing her discussing using critical race and whiteness theory, Kelly argues that private and governmental coalitional interests are at play in land rights reform. This in turn reiterates a relationship between neoliberalism and social conservatism which prompts the question: in whose interests is this “growth”?Also in the Australian context, Anthony Lambert’s paper “Rainbow Blindness” filters contemporary government attitudes and legislative change with respect to marriage and same-sex relationships through the effects of recent coalitional changes in the Australian and global political landscape. Lambert argues that the confusion surrounding the issue of gay marriage and the blurring/changes within political positions constitutes Australia as living within a ‘post-coalitional’ framework – one defined by persistence and change, where a new sensibility towards equity and difference is accompanied by the reassertion of larger coalitional affiliations and normative regimes. Duncan McKay’s paper sees a coalitional model of engagement as potentially providing productive possibilities between governmental bodies and the Western Australian Arts community. McKay passionately critiques the WA Department of Culture and the Arts (DCA) policy document Creating Value arguing that it “may be considered that the DCA and many WA cultural producers may not be engaged in the same project at all, let alone be in effective partnership or coalition”. Blair McDonald’s poetic contribution contends that out of a reading of Foucauldian resistance to sexual norms, new coalitions of behaviour and identity may be possible. In a coalitional context, Foucault cannot and does not simply seek to exit the networks of power and sexuality that he himself constructs. In retracing Foucault’s attention to power and sex-desire, the author seeks a movement toward “new coalitions” or “rallying points” at the limits of bodies and pleasures; in the bodies that are as yet ”unformed” and pleasures that are as yet “unknown”. Meanwhile, Lauren Cruikshank’s “Synaestheory: Fleshing Out a Coalition of the Senses” demonstrates how understanding the relationship between senses as coalitional breaks down the Cartesian dominance regarding subjectivity as exemplified by the mind/body split. Cruikshank’s careful analysis also challenges the privileging of vision in Western culture. As noted above, around the world, many new coalitional minority governments have taken power in recent times. In Australia, Christopher Payne of the Liberal Party referred to the negotiations following the August 2010 election (which resulted in a hung parliament) as Labor, the Independents and the Greens “trying to put together a coalition of the mongoose and the cobra” (ABC). Here, Payne attempts to cast doubt over the stability of this sort of coalition, by positing the Greens as the cobra and the Independents as potential pray to be attacked and devoured. More importantly, Payne has referenced, as this collection of papers does, the changeability of coalitions, and the sometimes antagonistic relationships that may need to co-exist in coalitions of all kinds. ReferencesAustralian Broadcasting Authority (ABC) Online News. “Pyne Warns of Labor ‘Mongoose and Cobra Coalition.” 4 Sep. 2010. 1 Dec. 2010 ‹http://www.abc.net.au/news/stories/2010/09/04/3002524.htm›.

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