Academic literature on the topic 'Native virus'
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Journal articles on the topic "Native virus"
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Tanaka, Y., E. Orito, T. Kato, S. Sonoda, K. Ohba, T. Miura, Y. Kondo, et al. "GB virus C/hepatitis G virus infection among Colombian native Indians." American Journal of Tropical Medicine and Hygiene 59, no. 3 (September 1, 1998): 462–67. http://dx.doi.org/10.4269/ajtmh.1998.59.462.
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Petrovic, Tamas, Sava Lazic, Milovan Jovicin, and Bosiljka Djuricic. "Possibility for use of RT-PCR technique in establishing presence of bovine viral diarrhea virus in sperm of breeding bulls." Veterinarski glasnik 59, no. 3-4 (2005): 371–81. http://dx.doi.org/10.2298/vetgl0504371p.
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The bovine viral diarrhea (BVD) virus is a significant health-economic pathogen in cattle which can be excreted and spread also through sperm of persistently or acutely infected bulls. Native sperm of 6 bulls, found to be negative to the BVD virus by isolating the virus and using the RT-PCR method, was experimentally infected with a tenfold dilution of the non-cytopathogen 22146 strain of the BVD virus with a titer of 105,5. This way, dilutions of the BVD virus from 10-1 to 10-6 (5 x 104 TCID/50 do 0,5 TCID/50 in 0.1 ml native sperm were obtained. From sperm infected in this way, the virus was reisolated on FTB cell culture in a microtiter plate with 96 pools in which each sample of the infected sperm was set up in three samples, and each of them was titrated to a dilution of 1:2 to 1:256. The presence of the BVD virus was proven using the technique of fluorescent antibodies in a second blind passage on FTB culture cells. For cell culture an extremely toxic effect of native sperm to a dilution of 1:64 was established. The BVD virus was reisolated from sperm in all three sperm samples with 5 x 104, 5 x 103 i 5 x 102 TCID/50, and it was not reisolated from sperm with 50, with 5, and with 0.5 TCID/50 BVD virus in 0.1 ml native sperm. At the same time, the presence of the BVD viral genome was proved using the RT-PCR method in the same samples of artificially infected native sperm of bulls. A positive re suit was established in native sperm with 5 x 104, 5 x 103, 5 x 102 and 50 TCID/50 BVD virus n 0.1 ml native sperm. The experiment proved that the RT-PCR method has advantages over the isolation of the BVD virus from samples of native sperm of bulls. These are: shortterm investigations (1 to 2 days) and greater sensitivity (10 times bigger than the isolation of the virus). The isolation of the virus takes at least 10 days, and its greater sensitivity is primarily a result of the cyrotoxic effect of native sperm of bulls on cell culture.
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Heinig-Hartberger, Mareike, Fanny Hellhammer, David D. J. A. Zöller, Susann Dornbusch, Stella Bergmann, Katerina Vocadlova, Sandra Junglen, Michael Stern, Kwang-Zin Lee, and Stefanie C. Becker. "Culex Y Virus: A Native Virus of Culex Species Characterized In Vivo." Viruses 15, no. 1 (January 14, 2023): 235. http://dx.doi.org/10.3390/v15010235.
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Mosquitoes are vectors of various pathogens that cause diseases in humans and animals. To prevent the outbreak of mosquito-borne diseases, it is essential to control vector populations, as treatment or vaccination for mosquito-borne diseases are often unavailable. Insect-specific viruses (ISVs) have previously been described as being potentially helpful against arboviral disease outbreaks. In this study, we present the first in vivo characterization of the ISV Culex Y virus (CYV). CYV was first isolated from free-living Culex pipiens mosquitoes in 2010; then, it was found in several mosquito cell lines in a further study in 2018. For mammalian cells, we were able to confirm that CYV does not replicate as it was previously described. Additionally, we found that CYV does not replicate in honey bees or locusts. However, we detected replication in the Culex pipiens biotype molestus, Aedes albopictus, and Drosophila melanogaster, thus indicating dipteran specificity. We detected significantly higher mortality in Culex pipiens biotype molestus males and Drosophila melanogaster, but not in Aedes albopictus and female Culex pipiens biotype molestus. CYV could not be transmitted transovarially to offspring, but we detected venereal transmission as well as CYV in mosquitos’ saliva, indicating that an oral route of infection would also be possible. CYV’s dipteran specificity, transmission routes, and killing effect with respect to Culex males may be used as powerful tools with which to destabilize arbovirus vector populations in the future.
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Callaway, Ewen. "Flu virus finally sequenced in its native form." Nature 556, no. 7702 (April 2018): 420. http://dx.doi.org/10.1038/d41586-018-04908-5.
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Deleersnyder, V., A. Pillez, C. Wychowski, K. Blight, J. Xu, Y. S. Hahn, C. M. Rice, and J. Dubuisson. "Formation of native hepatitis C virus glycoprotein complexes." Journal of virology 71, no. 1 (1997): 697–704. http://dx.doi.org/10.1128/jvi.71.1.697-704.1997.
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Ke, Zunlong, Rebecca S. Dillard, Cheri M. Hampton, Rachel E. Storms, Joshua D. Strauss, and E. R. Wright. "Native-State Structural Analysis of Respiratory Syncytial Virus." Microscopy and Microanalysis 22, S3 (July 2016): 1116–17. http://dx.doi.org/10.1017/s1431927616006425.
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Sharma, Shree G., Volker Nickeleit, Leal C. Herlitz, Anne K. de Gonzalez, Michael B. Stokes, Harsharan K. Singh, Glen S. Markowitz, and Vivette D. D'Agati. "BK polyoma virus nephropathy in the native kidney." Nephrology Dialysis Transplantation 28, no. 3 (December 18, 2012): 620–31. http://dx.doi.org/10.1093/ndt/gfs537.
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Kaito, Masahiko, Hiroyoshi Ohba, Joe Chiba, Michinori Kohara, Hideaki Tanaka, Naoki Fujita, Esteban Cesar Gabazza, Shozo Watanabe, Masayoshi Konishi, and Yukihiko Adachi. "The ultrastructural morphology of native hepatitis B virus." Medical Molecular Morphology 39, no. 3 (September 26, 2006): 136–45. http://dx.doi.org/10.1007/s00795-006-0330-y.
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Okoli, Uzoamaka Adaobi. "A Preliminary Investigation of Viral Pathogen Causing Foetal Abortion in Donkeys at Ethiopia." Biosciences, Biotechnology Research Asia 14, no. 3 (September 25, 2017): 1063–66. http://dx.doi.org/10.13005/bbra/2542.
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ABSTRACT: Rabbit kidney (RK13) cells infected with virus isolated from a homogenate made from the placenta of aborted foetal donkeys were stained with Leishman’s stain, morphological changes including cytopathic effects (CPE), syncytia, and inclusion bodies were seen by light microscopy after incubation for 24hrs-96hrs at 370C. After 48hrs of incubation, about 60% of cells were infected. Another Set of RK 13 cells infected with either native virus or both ether treated virus and native virus in the presence of acyclovir was stained with Giemsa, morphology changes were observed in the native virus infected cell while little or no change was seen in infected cells in the presence of acyclovir and ether treated virus respectively. Virus infected RK13 cells were stained with acridine orange, intracellular fluorescent green colour was seen by fluorescence microscopy in the cell nucleus. The clinical history and CPE of the virus in RK13 cell are similar to Equine Herpes virus.
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Trybala, Edward, Nadia Peerboom, Beata Adamiak, Malgorzata Krzyzowska, Jan-Åke Liljeqvist, Marta Bally, and Tomas Bergström. "Herpes Simplex Virus Type 2 Mucin-Like Glycoprotein mgG Promotes Virus Release from the Surface of Infected Cells." Viruses 13, no. 5 (May 12, 2021): 887. http://dx.doi.org/10.3390/v13050887.
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The contribution of virus components to liberation of herpes simplex virus type 2 (HSV-2) progeny virions from the surface of infected cells is poorly understood. We report that the HSV-2 mutant deficient in the expression of a mucin-like membrane-associated glycoprotein G (mgG) exhibited defect in the release of progeny virions from infected cells manifested by ~2 orders of magnitude decreased amount of infectious virus in a culture medium as compared to native HSV-2. Electron microscopy revealed that the mgG deficient virions were produced in infected cells and present at the cell surface. These virions could be forcibly liberated to a nearly native HSV-2 level by the treatment of cells with glycosaminoglycan (GAG)-mimicking oligosaccharides. Comparative assessment of the interaction of mutant and native virions with surface-immobilized chondroitin sulfate GAG chains revealed that while the mutant virions associated with GAGs ~fourfold more extensively, the lateral mobility of bound virions was much poorer than that of native virions. These data indicate that the mgG of HSV-2 balances the virus interaction with GAG chains, a feature critical to prevent trapping of the progeny virions at the surface of infected cells.
Dissertations / Theses on the topic "Native virus"
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Young, Katie. "Development of native and recombinant mumps virus subunit nasal vaccines using Protollin technology." Thesis, McGill University, 2009. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=92188.
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We sought to develop an inactivated nasal mumps virus (MuV) vaccine combined with the Protollin (Prl) adjuvant/delivery system. Antigen based on split Jones MuV was produced and characterized.Eight-week old BALB/c female mice were vaccinated with two or three doses of MuV antigen (4 or 8 micrograms) with or without 4 micrograms of Prl. Weight and behaviours were monitored to assess safety, and serum, respiratory secretions and splenocytes were obtained at study termination to assess MuV-specific immunity.
All vaccines were well-tolerated. Administration of 8 micrograms of MuV-Prl induced greater serum and mucosal antibodies than MuV antigen alone. MuV-Prl vaccines seemed to favour a Th1-type immune environment. Serum antibodies induced were capable of neutralizing MuV in vitro.
The intranasal MuV-Prl vaccine was safe and immunogenic. Future work will focus on the development of a trivalent MMR-Prl vaccine. Such a vaccine will be of great interest to the global health community.
Nous avons voulu explorer la faisabilité d'un vaccin contre le virus des oreillons (VdO) inactivé et administré par voie intra-nasale, et combiné avec l'adjuvant Protollin (Prl). Notre laboratoire a généré et a caractérisé des antigènes de virion entier désintégré utilisant un détergent. Des souris femelles de souche BALB/c âgées de huit semaines ont été vaccinées avec deux ou trois doses de VdO désintégré en antigène (4 ou 8 µg), avec ou sans 4 µg Prl. Les souris ont été suivies afin d'évaluer l'innocuité; des sérums et des sécrétions des muqueuses ont été obtenus à des intervalles afin d'évaluer l'immunité spécifique de VdO.
Tous les vaccins ont été bien tolérés chez les souris. Les vaccins VdO-Prl ont produit un plus grand taux d'IgG sériques et IgA au niveau de la muqueuse comparés aux vaccins VdO utilisés seuls. Les vaccins VdO-Prl ont tendance à générer une réponse immunitaire déviée sur Th1. Les anticorps sériques étaient capables de neutraliser le VdO.
Nous avons démontré que l'ensemble des vaccins de virus inactivés VdO-Prl administrés par voie intra-nasale est sans danger est immunogénique. Nous voulons générer un vaccin inactivé trivalent rougeole-oreillons-rubéole combiné avec le Prl. Un tel vaccin serait utile.
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Abroi, Aare. "The determinants for the native activities of the bovine papilloma virus type 1 E2 protein are separable /." Online version, 2004. http://dspace.utlib.ee/dspace/bitstream/10062/1348/5/abroi.pdf.
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Leggewie, Mayke [Verfasser], and Tim-Wolf [Akademischer Betreuer] Gilberger. "Susceptibility of Culex species native to Germany for West Nile virus and the role of Wolbachia in virus-vector interaction / Mayke Leggewie ; Betreuer: Tim-Wolf Gilberger." Hamburg : Staats- und Universitätsbibliothek Hamburg, 2017. http://d-nb.info/1148650571/34.
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Matanin, Brad Matthew. "Purification of the major envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions." Thesis, Virginia Tech, 2007. http://hdl.handle.net/10919/33194.
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Porcine reproductive and respiratory syndrome virus (PRRSV) is the cause of a pandemic that has been devastating the U.S. and global swine industry for more than twenty years. PRRSV vaccine development is challenging due to virus heterogeneity. Evidence indicates that the major envelope protein, GP5, is the primary target for a subunit vaccine. In native virions GP5 primarily exists as a disulfide linked complex with the membrane protein, M, which also possesses immunogenic properties. Recent studies report that the GP5/M complex is a more significant vaccine candidate. Currently, no bulk purification methods have been reported for PRRSV proteins. The objective of this research was to develop a purification process for GP5 or GP5/M from native virions.
PRRS virions were isolated and concentrated through sucrose cushion ultracentrifugation and target envelope proteins were solubilized with Triton X-100 detergent for further processing. GP5/M was not consistently identified in samples and was therefore abandoned. GP5 was identified by Western blot throughout processing with a αORF5 antibody. Cation exchange chromatography (CEX) was utilized for partial fractionation of GP5, although the viral nucleocapsid protein, N, was a major impurity in CEX elution fractions. As a second chromatographic step, hydrophobic interaction chromatography (HIC) further purified GP5 by means of a two-stage elution scheme. Pure GP5 was eluted from the HIC resin in the second HIC elution stage by Triton X-100 displacement; however the protein is present as a homodimeric/tetrameric aggregate. This process will be useful in PRRSV vaccine development and the purified GP5 product could be used as much needed positive controls in animal studies.Master of Science
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Jones, Taylor J. "Documentation of grapevine leafroll-associated viruses in wine grape varieties and native grape species in Virginia, and examination of the movement of grapevine leafroll disease to develop management strategies." Thesis, Virginia Tech, 2012. http://hdl.handle.net/10919/49567.
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Grapevine leafroll-associated virus-2 (GLRaV-2), GLRaV-3, and grapevine fleck virus (GFkV) are widespread in grapes around the world. These viruses can cause significant crop loss and affect wine quality by reducing sugar accumulation and compromising skin color. Mealybugs are vectors of grapevine leafroll-associated viruses (GLRaVs). A statewide survey of commercial and wild grapevines in Virginia was conducted during 2009 through 2011. Also, vector management options were tested in two field studies. GLRaV-2, GLRaV-3, and GFkV were detected in 8%, 25%, and 1%, respectively, of over 1,200 vine samples (41 wine grape varieties) from 77 locations, and 64% of vineyards were positive for at least one of the tested viruses. All 100 wild grapevines tested were free of these three viruses, indicating that they are not alternative hosts. The majority of infected vines from commercial vineyards were planted prior to the 1990\'s; however, some new plantings were also found to be positive, indicating movement of the viruses among vineyards and also potential infection prior to planting. The high frequency of virus-infected vines emphasizes the importance of clean plant materials, as well as management of vector insects. The insecticide trials resulted in promising vector control with dinotefuran and spirotetramat; however, acetamiprid and pryrethroid resulted in an increase in mealybug population. This study is the first to examine multiple grape viruses in VA. It will aid in developing better strategies aimed at controlling mealybugs to restrict the movement of viral diseases.Master of Science in Life Sciences
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Tangkanond, Wipa. "Molecular Evolution of Japanese Encephalitis Virus in Nature." Thesis, University of Liverpool, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.526948.
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Atlan, Hervé. "Nature et origine des virus mammifères présents dans les eaux de surface." Paris 5, 1991. http://www.theses.fr/1992PA05P043.
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Masiri, Jongkit Murphy John F. "The nature of cucumber mosaic virus-induced symptoms in bell pepper (Capsicum annuum L.)." Auburn, Ala., 2009. http://hdl.handle.net/10415/1977.
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Asghar, Naveed. "Ticks and Tick-borne Encephalitis Virus : From Nature to Infection." Doctoral thesis, Södertörns högskola, Miljövetenskap, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:sh:diva-31153.
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Vector-borne diseases are an increasing global threat to humans due to climate changes, elevating the risk of infections transmitted by mosquitos, ticks, and other arthropod vectors. Ixodes ricinus, a common tick in Europe, transmits dangerous tick-borne pathogens to humans. Tick-borne encephalitis (TBE) is a vector-borne disease caused by TBE virus (TBEV). Climate change has contributed to increased tick abundance and incidence of tick-borne diseases, and between 10,000 and 15,000 human TBE cases are reported annually in Europe and Asia. TBEV shows a patchy geographical distribution pattern where each patch represents a natural focus. In nature, TBEV is maintained within the tick-rodent enzootic cycle. Co-feeding is the main route for TBEV transmission from infected to uninfected ticks and for maintenance within the natural foci. The increasing number of TBE cases in Scandinavia highlights the importance of characterizing additional TBEV sequences and of identifying novel natural foci, and in this work we sequenced and phylogenetically characterized four TBEV strains: Saringe-2009 (from a blood-fed nymph), JP-296 (from a questing adult male), JP-554 (from a questing adult male), and Mandal-2009 (from a pool of questing nymphs, n = 10). Mandal-2009 represents a TBEV genome from a natural focus in southern Norway. Saringe-2009 is from a natural endemic focus in northern Stockholm, Sweden, and JP-296 and JP-554 originate from a natural focus “Torö” in southern Stockholm. In addition, we have studied the effect of different biotic and abiotic factors on population dynamics of I. ricinus in southern Stockholm and observed significant spatiotemporal variations in tick activity patterns. Seasonal synchrony of immature stages and total tick abundance are important factors for the probability of horizontal transmission of TBEV among co-feeding ticks. We found that the probability of co-occurrence of larvae, nymphs, and female adults was highest during early summer whereas increasing vegetation height and increasing amounts of forest and open water around the study sites had a significant negative effect on co-occurrence of larvae, nymphs, and female adults. The proximal part of the 3 ́non-coding region (3 ́NCR) of TBEV contains an internal poly(A) tract, and genomic analysis of Saringe-2009 revealed variability in the poly(A) tract indicating the existence of different variants within the TBEV pool of Saringe-2009. Like other RNA viruses, TBEV exists as swarms of unique variants called quasispecies. Because Saringe-2009 came from an engorged nymph that had been feeding on blood for >60 h, we propose that Saringe-2009 represents a putative shift in the TBEV pool when the virus switches from ectothermic/tick to endothermic/mammalian environments. We investigated the role of poly(A) tract variability in replication and virulence of TBEV by generating two infectious clones of the TBEV strain Toro-2003, one with a short/wild-type (A)3C(A)6 poly(A) tract and one with a long (A)3C(A)38 poly(A) tract. The infectious clone with the long poly(A) tract showed poor replication in cell culture but was more virulent in C57BL/6 mice than the wild-type clone. RNA folding predictions of the TBEV genomes suggested that insertion of a long poly(A) tract abolishes a stem loop structure at the beginning of the 3 ́NCR. Next generation sequencing (NGS) analysis of the TBEV genomes after passaging in cell culture and/or mouse brain revealed molecular determinants and quasispecies structure that might contribute to the observed differences in virulence. Our findings suggest that the long poly(A) tract imparts instability to the TBEV genome resulting in higher quasispecies diversity that in turn contributes to TBEV virulence. Phylogenetic analysis of Saringe-2009, JP-296, JP-554, and Mandal-2009 predicted a strong evolutionary relationship among the four strains. They clustered with Toro-2003, the first TBEV strain from Torö, demonstrating a Scandinavian clade. Except for the proximal part of the 3 ́NCR, TBEV is highly conserved in its genomic structure. Genomic analysis revealed that Mandal-2009 contains a truncated 3 ́NCR similar to the highly virulent strain Hypr, whereas JP-296 and JP-554 have a genomic organization identical to Toro-2003, the prototypic TBEV strain from the same natural focus. NGS revealed significantly higher quasispecies diversity for JP-296 and JP-554 compared to Mandal-2009. In addition, single nucleotide polymerphism (SNP) analysis showed that 40% of the SNPs were common between quasispecies populations of JP-296 and JP-554, indicating the persistence and maintenance of TBEV quasispecies within the natural focus. Taken together, these findings indicate the importance of environmental factors for the occurrence pattern of the different life-stages of the tick vector, which are important for the persistence of TBEV in nature. Our findings also show that the selection pressure exerted by specific host also affects the population structure of the TBEV quasispecies. In addition, our results further demonstrate that the evolution of quasispecies has effect on TBEV virulence in mice.Vektorburna sjukdomar är ett växande globalt hot mot både människor och djur. De pågående klimatförändringarna kan leda till förhöjda risker för infektioner överförda av myggor, fästingar och andra leddjursvektorer. Ixodes ricinus är en vanlig fästing i Europa som överför fästingburna patogener som är farliga för människor. Fästingburen encefalit (TBE) är en vektorburen sjukdom som orsakas av TBE-virus (TBEV). De pågående klimatförändringarna har bidragit till en ökning både av vektorn och sjukdomsfrekvensen. Mellan 10 000 och 15 000 mänskliga TBE-fall rapporteras årligen i Europa och Asien. Den geografiska fördelningen av TBEV visar ett ojämnt fördelningsmönster där viruset är koncentrerat till vissa fokusområden. TBEV återfinns i naturen i en livscykel där viruset hela tiden överförs mellan fästingar och däggdjur. Spridningen sker dels från en infekterad fästing till ett ryggradsdjur när fästingen äter på värddjuret. Spridning mellan fästingar sker troligen främst genom så kallad “co-feeding”, det vill säga att flera fästingar suger blod samtidigt från samma värddjur. Viruset kan då passera från en infekterad fästing, genom värddjuret till oinfekterade fästingar. Virus kan identifieras och studeras med genetiska metoder. Det ökande antalet TBE-fall i Skandinavien styrker vikten av att hitta och karakterisera ytterligare TBEV-stammar och identifiera nya naturliga fokusområden. Vi har sekvenserat och fylogenetiskt beskrivit fyra TBEV-stammar: Saringe-2009 (blodfylld nymf), JP-296 (födosökande vuxen hane), JP-554 (födosökande vuxen hane) och Mandal-2009 (födosökande nymfer, n = 10). Mandal-2009 är ett TBEV från ett naturligt fokusområde i södra Norge. Saringe-2009 kommer från ett naturligt fokusområde i norra Stockholms län, Sverige. JP-296 och JP-554 härstammar från Torö som är ett naturligt fokusområde i södra Stockholms län, Sverige. Förutom den genetiska sekvenseringen av TBEV har vi också studerat effekten av olika biotiska och abiotiska faktorer på populationsdynamik av I. ricinus i södra Stockholm och observerade variation i fästingsaktivitetsmönster både temporalt och spatialt. Förekomstmönster av fästinglarver, nymfer och vuxna honor, och det totala antalet fästingar är viktiga faktorer för sannolikheten för horisontell överföring av TBEV mellan fästingar. Vi fann att sannolikheten för synkron förekomst av larver, nymfer och honor var högst under försommaren. Vegetationshöjd, mängden skog och mängd öppet vatten runt undersökningsområden hade signifikanta negativa effekter på sannolikheten för att larver, nymfer och honor skulle förekomma samtidigt. Den variabla delen av den icke-kodande 3 ́regionen (3'NCR) av TBEV-genomet innehåller ofta en intern poly(A)-sekvens. Liksom andra RNA-virus, förekommer TBEV som så kallade ”quasispecies” vilka definieras som grupper av olika genetiska varianter av virus. Genom analysen av TBEV-stam Saringe-2009 avslöjades variation i poly(A)-sekvensen vilket indikerar förekomst av ”quasispecies”. Eftersom Saringe-2009 kom från en blodfylld nymf som hade sugit blod i > 60 timmar, föreslår vi att Saringe-2009 visar en förändring i ”quasispecies”-poolen när viruset överförs från exoterm fästingmiljö till endoterm däggdjursmiljö. Vi undersökte poly(A)-ekvensens variabilitet och dess roll vid replikering och för virulens hos TBEV, genom att skapa två infektiösa kloner av Torö-2003 stammen; en med en kort/vild-typ (A)3C(A)6 poly(A)-sekkvens, och en med en lång (A)3C(A)38 poly(A)-sekvens. Den infektiösa klonen med lång poly(A)-sekvens replikerade sämre än vildtypklonen i cellkultur, men (A)3C(A)38 poly(A) var mer virulent i C57BL/6-möss än (A)3C(A)6 poly(A). Datasimulering av TBEV-genomets sekundär-RNA-struktur visade att de längre poly(A)-sekvenserna påverkar veckningen av en specifik sekundärstruktur (SL14) i början av 3 ́NCR. Djupsekvenseringsanalys av TBEV-gnomen avslöjade skillnader för specifika gener och ”quasispecies”-strukturen efter passering i cellkultur och/eller mushjärna. Dessa förändringar föreslås bidra till de observerade skillnaderna i virulens. Våra resultat indikerar att den långa poly(A)-sekvensen ger instabilitet i TBEV-genomet, vilket resulterar i ökad mångfald av ”quasispecies”-populationen som i sin tur kan bidra till TBEV-virulens. Fylogenetisk analys av Saringe-2009, JP-296, JP-554 och Mandal-2009 visade på ett nära släktskap mellan de fyra skandinaviska TBEV-stammarna. De nya stammarna formerade ett kluster med en tidigare TBEV-stam identifierad på Torö (Toro-2003), vilket skapade ett skandinaviskt klad. Genetisk analys visade att Mandal-2009 innehåller en trunkerad 3 ́NCR som liknar den högvirulenta stammen HYPR. JP-296 och JP-554 hade däremot samma genetiska struktur som den längre Torö-2003 stammen från samma fokusområde. Djupsekvensering visade höge mångfald av ”quasispecies”-populationen för JP-296 och JP- 554 jämfört med Mandal-2009. Analys av enkel nukleotid polymorfism (SNP) visade att 40 % av alla SNP var gemensamma mellan ”quasispecies”-populationen för JP-296 och JP-554. Detta indikerar att TBEV-”quasispecies”-strukturen kan vara konserverad för närbesläktade virus vilken kan leda till att den bevaras inom specifika fokusområden. Sammantaget så visar dessa studier att miljöfaktorer påverkar förekomsten av fästingvektorn och dess olika livsstadier, vilket är en bakomliggande faktor för utbredning av TBEV i naturliga fokusområden. Det visar även på att värdmiljön påverkar strukturen för ”quasispecies”-populationen. Dessutom visar våra studier att evolution och utveckling av ”quasispecies”-strukturen kan påverka virulensen för TBEV i möss.
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Godoy, Bibiane Armiliato. "História evolutiva do vírus da hepatite B em populações nativas americanas." reponame:Biblioteca Digital de Teses e Dissertações da UFRGS, 2014. http://hdl.handle.net/10183/129490.
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Introdução: O Vírus da Hepatite B (HBV) é um vírus de DNA com tropismo por hepatócitos, que apresenta um genoma circular parcialmente dupla fita. Baseado na divergência de sequência do genoma completo, dez linhagens evolutivas, denominadas “genótipos” de HBV, foram descritas (A-J), sendo F e H considerados como “indígenas” da América. Os genótipos de HBV apresentam uma forte estruturação geográfica, o que pode refletir padrões das migrações humanas. Na América do Sul, áreas de alto endemismo incluem a região amazônica, e as maiores taxas de infecção têm sido observadas em populações Nativas Americanas. Embora a forte estruturação geográfica seja indicativa de uma origem antiga, a maioria das análises visando datar a origem dos genótipos “americanos” F e H resulta em datações extremamente recentes que não condizem com eventos históricos relacionados ao HBV. Objetivo: Os objetivos desse trabalho compreendem avaliar o impacto de diferentes taxas evolutivas e da seleção purificadora sobre as estimativas de datação molecular a fim de inferir quais taxas são mais condizentes com a origem do HBV na América; caracterizar o HBV circulante em uma amostra histórica de Nativos Americanos, e discutir os processos históricos que possam ser relevantes para entender os padrões observados. Material e Métodos: Nós realizamos análise Bayesiana utilizando sequências disponíveis dos genótipos F e H e diferentes taxas evolutivas previamente reportadas, e comparamos a ocorrência de mutações sinônimas e não-sinônimas em ramos da filogenia classificados como “antigos” ou “recentes” a fim de inferir a atuação da seleção purificadora ao longo do tempo. Para caracterização do HBV presente nas populações Nativas Americanas, detecção e amplificação do DNA viral foi obtida através de PCR seguido de sequenciamento e análise filogenética. Análise Bayesiana de Skyline Plot foi realizada para comparar a dinâmica populacional do subgenótipo A1 presente na amostra de Nativos Americanos e em outras cepas isoladas no Brasil. Resultados e conclusão: Nossos resultados mostram que as estimativas de datação molecular são fortemente influenciadas pelas taxas evolutivas utilizadas na análise. Além disso, foi observado excesso de mutações não-sinônimas nos ramos recentes da filogenia, o que é compatível com a ocorrência de seleção purificadora, e pode gerar um viés sobre as estimativas, produzindo datações recentes demais. Na amostra de Nativos Americanos, nós constatamos o predomínio do subgenótipo A1, relacionado com populações africanas. Análise de Skyline Plot mostrou que a expansão populacional nas cepas isoladas de Nativos Americanos é mais recente que aquela inferida para outras cepas brasileiras, sugerindo que os processos históricos que contribuíram para a formação do subgenótipo A1 dos Nativos Americanos são relacionados com ondas migratórias mais recentes em direção à região amazônica.Introduction: Hepatitis B virus (HBV) is a hepatotropic DNA virus that presents a partially double-stranded circular genome. Based on sequence divergence of the complete genome, ten HBV evolutionary lineages, called “genotypes” have been described (A-J), with F and H being considered as indigenous from the Americas. HBV genotypes present a remarkable geographic structure which may reflect historic patterns of human migrations. In South America, areas of high endemism include the Amazon basin, and high prevalence rates have been observed in Native American populations. Although the strong geographical structure indicates an ancient origin, most analysis trying to date the origin of the “American” genotypes F and H result in extremely recent dates that disagree with historical events related with HBV. Objective: The aims of this study comprise evaluate the impact of different evolutionary rates and of the purifying selection on molecular dating estimates in order to infer which rates are in better agreement with the origin of HBV in the Americas; to characterize the HBV circulating in a historical sample of Native Americans, and discussing the historical processes that might be relevant to understand the observed patterns. Materials and Methods: We performed a Bayesian analysis using the available sequences of F and H genotypes and different evolutionary rates previously reported, and compared the occurrence of synonymous and non-synonymous mutations in branches of phylogeny classified as “old” or “young” in order to infer the effects of purifying selection over time. For the characterization of HBV from Native American populations, detection and amplification of viral DNA were obtained through PCR followed by sequencing and phylogenetic analysis. Bayesian Skyline Plot analysis was performed to compare the population dynamics of the A1 subgenotype present in the sample of Native American and in other strains isolated from Brazil. Results and Conclusion: Our results show that molecular dating estimates are strongly influenced by the evolutionary rate assumed in the analysis. In addition, we observed an excess of non-synonymous mutations in recent branches of phylogeny, which is compatible with the occurrence of purifying selection and may create a bias on the estimates, producing too recent datings. In the sample of Native Americans, we observed a predominance of the A1 subgenotype, related with African populations. Skyline Plot analysis showed that population expansion in strains isolated from Native Americans is more recent than that inferred from other Brazilian strains, suggesting that the historical processes that contributed to the presence of A1 subgenotype A1 Native Americans are related with more recent migratory waves towards the Amazon region.
Books on the topic "Native virus"
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Bānēt, Paphāsiri. Rāingān chabap sombūn rư̄ang kānphrǣkračhāi læ kānthāithō̜t chư̄a wairat rawāng kung khāo kap kung phư̄nmư̄ang nai lum Mǣnam Bāng Pakong =: Distribution and transmission of virus diseases between Litopenaeus vannamei and native shrimp species in Bangpakong watershed. [Bangkok]: Samnakngān Khana Kammakān Wičhai hǣng Chāt, 2007.
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Bānēt, Paphāsiri. Rāingān khrōngkān wičhai pī thī 2 rư̄ang kānphrǣ kračhāi læ kānthāithō̜t chư̄a wairat rawāng kung khāo kap kung phư̄nmư̄ang nai lum mǣnam Bāng Pakong =: Distribution and transmission of virus diseases between Litopenaeus vannamei and native shrimp species in Bangpakong watershed. [Bangkok?]: Samnakngān Khana Kammakān Wičhai hǣng Chāt, 2006.
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Jack Blank and the Imagine Nation. New York: Aladdin, 2010.
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Diamond, Jared M. Vete, vapen & virus: En kort sammanfattning av mänsklighetens historia under de senaste 13 000 åren. Stockholm: Norstedt, 2006.
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International Symposium on Bluetongue, African Horse Sickness, and Related Orbiviruses (2nd 1991 Paris, France). Bluetongue, African horse sickness, and related orbiviruses: Proceedings of the Second International Symposium. Boca Raton: CRC Press, 1992.
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International Symposium on Bluetongue, African Horse Sickness, and Related Orbiviruses (2nd 1991 Paris, France). Bluetongue, African horse sickness and related orbiviruses: 2nd International Symposium, Paris, 17-21 June 1991 : summary and conclusions. Paris: Office international des epizooties, 1992.
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Dashner, James. Si wang jie yao. Taibei Shi: San cai wen hua chu ban shi ye you xian gong si, 2014.
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Dashner, James. The Death Cure. Somerset, UK: Chicken House, 2012.
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The death cure. Frome, Somerset: Chicken House, 2015.
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Schwemmer, Renae Marie. Ones We Left Behind: When a Virus Spread, a Nation Fell. Independently Published, 2020.
Find full textBook chapters on the topic "Native virus"
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Rademacher, Christoph, and Thomas Peters. "Molecular Recognition of Ligands by Native Viruses and Virus-Like Particles as Studied by NMR Experiments." In Topics in Current Chemistry, 183–202. Berlin, Heidelberg: Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/128_2007_19.
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Michael, T., A. Wilson, Keith Saunders, Mandy J. Dowson-Day, David E. Sleat, Hans Trachsel, and Karl W. Mundry. "Effects of the 5′-Leader Sequence of Tobacco Mosaic Virus RNA, or Derivatives Thereof, on Foreign mRNA and Native Viral Gene Expression." In Post-Transcriptional Control of Gene Expression, 261–75. Berlin, Heidelberg: Springer Berlin Heidelberg, 1990. http://dx.doi.org/10.1007/978-3-642-75139-4_25.
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Warziniack, Travis, Robert G. Haight, Denys Yemshanov, Jenny L. Apriesnig, Thomas P. Holmes, Amanda M. Countryman, John D. Rothlisberger, and Christopher Haberland. "Economics of Invasive Species." In Invasive Species in Forests and Rangelands of the United States, 305–20. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-45367-1_14.
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AbstractWhile the subset of introduced species that become invasive is small, the damages caused by that subset and the costs of controlling them can be substantial. This chapter takes an in-depth look at the economic damages non-native species cause, methods economists often use to measure those damages, and tools used to assess invasive species policies. Ecological damages are covered in other chapters of this book. To put the problem in perspective, Federal agencies reported spending more than half a billion dollars per year in 1999 and 2000 for activities related to invasive species ($513.9 million in 1999 and $631.5 million in 2000 (U.S. GAO 2000)). Approximately half of these expenses were spent on prevention. Several states also spend considerable resources on managing non-native species; for example, Florida spent $127.6 million on invasive species activities in 2000 (U.S. GAO 2000), and the Great Lakes states spend about $20 million each year to control sea lamprey (Petromyzon marinus) (Kinnunen 2015). Costs to government may not be the same as actual damages, which generally fall disproportionately on a few economic sectors and households. For example, the impact of the 2002 outbreak of West Nile virus exceeded $4 million in damages to the equine industries in Colorado and Nebraska alone (USDA APHIS 2003) and more than $20 million in public health damages in Louisiana (Zohrabian et al. 2004). Zebra mussels (Dreissena polymorpha) cause $300–$500 million annually in damages to power plants, water systems, and industrial water intakes in the Great Lakes region (Great Lakes Commission 2012) and are expected to cause $64 million annually in damages should they or quagga mussels (Dreissena bugensis) spread to the Columbia River basin (Warziniack et al. 2011).
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Crick, F. H. C., and J. D. Watson. "Virus Structure: General Principles." In Ciba Foundation Symposium - The Nature of Viruses, 5–18. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470715239.ch1.
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Morimoto, Daichi, Kento Tominaga, Hiroaki Takebe, Sigitas Šulčius, and Takashi Yoshida. "Viral Nature of the Aquatic Ecosystems." In The Biological Role of a Virus, 3–25. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-85395-2_1.
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Duesberg, Peter H., and Harvey Bialy. "Duesberg and the right of reply according to Maddox-Nature." In AIDS: Virus- or Drug Induced?, 111–25. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-1651-7_8.
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Koliadin, Vladimir L. "Critical analysis of the current views on the nature of AIDS." In AIDS: Virus- or Drug Induced?, 69–88. Dordrecht: Springer Netherlands, 1996. http://dx.doi.org/10.1007/978-94-009-1651-7_4.
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Hess, W. R. "African Swine Fever Virus in Nature." In African Swine Fever, 5–9. Boston, MA: Springer US, 1987. http://dx.doi.org/10.1007/978-1-4613-2343-3_2.
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Ada, G. L. "Ribonucleic Acid in Influenza Virus." In Ciba Foundation Symposium - The Nature of Viruses, 104–22. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470715239.ch7.
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Doherty, P. C., and S. J. Turner. "The virus-immunity ecosystem." In Infectious Diseases from Nature: Mechanisms of Viral Emergence and Persistence, 17–32. Vienna: Springer Vienna, 2005. http://dx.doi.org/10.1007/3-211-29981-5_3.
Full textConference papers on the topic "Native virus"
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Katz, Alvin, Alexandra Alimova, Paul Gottlieb, Rakhi Podder, Glenn Minko, Hue Wei, and Robert R. Alfano. "Virus-host interaction probed by native Stokes shift fluorescence." In Biomedical Optics 2004, edited by Anita Mahadevan-Jansen, Michael G. Sowa, Gerwin J. Puppels, Zygmunt Gryczynski, Tuan Vo-Dinh, and Joseph R. Lakowicz. SPIE, 2004. http://dx.doi.org/10.1117/12.528330.
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O'Connell, Kevin P., Patricia E. Anderson, Michael S. Horsmon, and James J. Valdes. "NATIVE AND ENGINEERED SIMULANTS FOR DNA VIRUS THREAT AGENTS." In Proceedings of the 24th US Army Science Conference. WORLD SCIENTIFIC, 2006. http://dx.doi.org/10.1142/9789812772572_0058.
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Peresunko, O. P., Ju G. Karpenko, D. N. Burkovets, P. V. Ivashko, A. V. Nikorych, S. B. Yermolenko, Ion Gruia, and M. J. Gruia. "Laser diagnostics of native cervix dabs with human papilloma virus in high carcinogenic risk." In 12th International Conference on Correlation Optics, edited by Oleg V. Angelsky. SPIE, 2015. http://dx.doi.org/10.1117/12.2228804.
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Berntorp, E., S. Lethagen, and I. M. Nilsson. "BIOCHEMICAL PROPERTIES OF COMMERCIAL, VIRUS-INACTIVATED FACTOR VIII CONCENTRATES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644055.
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As all commercial factor VIII concentrates in current use have been subjected to some form of virus-inactivation, we wanted to compare their in vitro biochemical characteristics. Of the 10 concentrates studied, inactivation takes the form of dry heat treatment, varying from 60°C for 30 h to 72°C for 68 h, in six products (Octonativ, KabiVitrum; Hemofil T, Hyland; Factorate HP, Armour - heated two different ways; Monoclate, Armour; Nordiocto, Nordisk Gentofte). In the 4 remaining concentrates, inactivation is either by steam treatment (Kryobulin Tim 3, Immuno), heating at 60°C for 20 h as dry material slammed in heptane (Profilate, Alpha), wet-heating at 60°C for 10 h (Hemate, Behring), or by the New York Blood Center (solvent/detergent) method (OCTA-V. I., Octopharm). The variables studied were VIII:C by one-stage and chromogenic assay, and VI11 :Ag, vW:Ag, by electroimmunoassay, immunoradiometric assay (IRMA), crossed immunoelectrophoresis and SDS agarose gel electrophoresis followed by staining with radioactive antibody and fibrinogen. VIII :C activity values ranged from 20-53 IU/ml in all products but Monoclate (94 IU/ml). All products gave higher values of VIII:Ag than of VI11 :C, indicating partial inactivation of VIII :C during preparation; the ratios ranged from 1.2 to 1.3 for Kryobulin, Nordiocto, OCTA-V. I., and Hemofil T, and from 2 to 4 for the other products, being highest in Profilate and Monoclate. Specific activity was higher in Monoclate, Nordiocto, Hemate, OCTA-V. I. and Factorate HP (16.0, 7.4, 7.1, 4.8 and 3.3 IU/ml protein, respectively) than in the other products (1.3-2.3 IU/mg). All concentrates contained vW:Ag, and non-parallel dose-response curves indicated abnormality in the vWF molecule in all cases; and thus multimeric sizing failed to demonstrate the largest multimers of the vWF in any product. We conclude that although virus-inactivated F VIII concentrates are general ly comparable with regard to VIII :C content, they vary considerably in the degree of VIII :C inactivation during preparation, and in specific activity. No concentrate tested here contained native vWF. In vivo studies have shown VIII :C recovery and half-life to be comparable in heat-treated and non-heat-treated concentrates.
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Chen, Lin, Xianju Chen, Xiankui Tan, Meng Li, Fang Hu, Haiyan Zhang, and Xiaoyuan Wang. "Abstract 4072: A combinational therapy of oncolytic virus and nature killer cells for melanoma treatment." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.sabcs18-4072.
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Chen, Lin, Xianju Chen, Xiankui Tan, Meng Li, Fang Hu, Haiyan Zhang, and Xiaoyuan Wang. "Abstract 4072: A combinational therapy of oncolytic virus and nature killer cells for melanoma treatment." In Proceedings: AACR Annual Meeting 2019; March 29-April 3, 2019; Atlanta, GA. American Association for Cancer Research, 2019. http://dx.doi.org/10.1158/1538-7445.am2019-4072.
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Mitra, Debanjan, and Pradeep K. Mohapatra. "Effect of natural compounds to inhibit human respiratory syncytial virus." In 7th GoGreen Summit 2021. Technoarete, 2021. http://dx.doi.org/10.36647/978-93-92106-02-6.18.
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Current COVID-19 effects are forcing us to think about other deadly viral diseases. Respiratory syncytial virus (RSV) is one of them. Every year thousands of children lost their lives due to respiratory diseases which are occurred by this RSV. Nowadays, bioactive compounds show an enormous effect on many deadly diseases and show excellent therapeutic effects. In this study, we have identified five bioactive compounds from the plant which will be used in the treatment of RSV. Molecular docking on the protein was done by Autodock. Hydrogen was added and routable bonds were fixed in the preparation time of protein for docking. All those compounds show their non-toxic nature which is evaluated by Lipinski's Rule of Five. Molecular docking on RSV matrix protein and surface glycoprotein with those bioactive compounds shows very promising results. Between all those compounds Baicalein appears as a lead compound. It shows -8.1 Kcal/mol in the case of matrix protein and -7.9 kcal/mol in the case of the surface glycoprotein of RSV. Due to its availability and non-toxic nature, it can be used in the treatment of RSV. AS it is derived from plants, it also has very fewer side effects than chemical drugs.
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Badamshina, G. G., E. P. Sizova, and L. M. Fatkhutdinova. "STUDY OF HUMORAL IMMUNITY TO INFECTIONS IN MEDICAL WORKERS." In The 16th «OCCUPATION and HEALTH» Russian National Congress with International Participation (OHRNC-2021). FSBSI “IRIOH”, 2021. http://dx.doi.org/10.31089/978-5-6042929-2-1-2021-1-44-47.
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Abstract: Introduction. In the course of their work, medical workers are exposed to a biological factor, including bacterial, viral nature. Medical personnel come into contact with patients with measles, rubella, diphtheria, tuberculosis, hepatitis, coronavirus infection and other infectious diseases. The aim of the study is to assess the humoral immunity by the presence antibodies to the measles, rubella, hepatitis B viruses, to the causative agent COVID-19, tuberculosis and diphtheria bacteria in health care workers. Methods. Antibodies to measles, rubella, hepatitis B viruses, diphtheria and tetanus pathogens were measured in blood serum samples of 1221 MW; total antibodies to mycobacterium tuberculosis - in 120 MW; antibodies to the nucleocapsid protein of the SARS-CoV-2 virus – in 301 MW. The study was carried out by the method of enzyme immunoassay using commercial test systems; antibodies to diphtheria toxoid were detected in the passive hemagglutination reaction. The control group consisted of persons of engineering and technical personnel, comparable in age, gender and work experience. Results. Medical personnel were found to have significantly more frequent detection of seronegative reactions to the presence of antibodies to the hepatitis B virus (40.9% and 13.5%, p<0.001) of measles (28.8% and 3.9%, p<0.05); significantly high prevalence in the presence of total antibodies to mycobacterium tuberculosis (7.5% of cases in medical, 0% of cases of workers in the control group, p<0.05). In comparison with doctors, nurses had a significantly higher prevalence of antibodies to the nucleocapsid of the SARS-CoV-2 virus (38.9% and 23.7%, p<0.05). Conclusions. The study of post-vaccination immunity in medical workers showed the presence of a high proportion of seronegative individuals among vaccinated (viral hepatitis B, measles) medical workers and, accordingly, significant biological risks. A higher seroprevalence in total antibodies to Mycobacterium tuberculosis may also indicate insufficient immune protection among MW. The biological significance of seroprevalence to SARS-CoV-2 virus proteins (for nurses) requires further study.
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Radić, Vlado, and Nikola Radić. "RISK MANAGEMENT CHALLENGES IN THE COVID-19 PANDEMIC." In Sixth International Scientific-Business Conference LIMEN Leadership, Innovation, Management and Economics: Integrated Politics of Research. Association of Economists and Managers of the Balkans, Belgrade, Serbia, 2020. http://dx.doi.org/10.31410/limen.2020.57.
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Even before the current pandemic, humanity was faced with numerous situations that had serious global consequences. In addition to wars, nuclear radiation, cataclysmic earthquakes, volcanic eruptions, tsunamis, epidemics of SARS, swine flu, MERS, HIV, Ebola, Zika virus, they led to the cognition that humanity is powerless in the face of such disasters. Regardless of the achievements and development of science and technology, extensive and long-lasting medical research, "invisible" enemies have taken millions of human lives. People have always been faced with a risk, which comes from nature, human activities, or the mistakes of the man himself. Risk is a multidimensional, multifaceted and complex phenomenon, present on a daily basis in human life. Risk management in a state of the pandemic is primarily aimed at preserving the health and lives of the entire population, and measures applied to prevent a pandemic from taking countless human lives have no alternative.
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Milovanović, Ana. "Mogućnosti i izazovi nastave dramske umetnosti u izmenjenom društvenom kontekstu." In Nauka, nastava, učenje u izmenjenom društvenom kontekstu. University of Kragujevac, Faculty of Education in Uzice, 2021. http://dx.doi.org/10.46793/nnu21.189m.
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The subject of the paper are the possibilities and challenges of teaching drama in a changed social context. In the social context changed by the application of the agenda of globalism in Serbian education, the teaching of drama for preschool teachers begins to lose quality at university. Due to the specific nature of drama as art, online teaching of this subject is not possible. Research of teaching the subject Drama and Movement at the Teacher Education Faculty in Belgrade was shown that teaching was limited to lectures and theoretical exercises during the state of emergency, and that practical exercises were necessary after its abolition. An insurmountable challenge was the protection measures against the corona virus, as a result of which it was impossible to hold exercises of puppet animation and puppet directing, ie to prepare puppet shows (except monodramas). High engagement of professor was necessary for the quality of teaching.
Reports on the topic "Native virus"
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Bercovier, Herve, and Ronald P. Hedrick. Diagnostic, eco-epidemiology and control of KHV, a new viral pathogen of koi and common carp. United States Department of Agriculture, December 2007. http://dx.doi.org/10.32747/2007.7695593.bard.
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Original objectives and revisions-The proposed research included these original objectives: field validation of diagnostic tests (PCR), the development and evaluation of new sensitive tools (LC-PCR/TaqManPCR, antibody detection by ELISA) including their use to study the ecology and the epidemiology of KHV (virus distribution in the environment and native cyprinids) and the carrier status of fish exposed experimentally or naturally to KHV (sites of virus replication and potential persistence or latency). In the course of the study we completed the genome sequence of KHV and developed a DNA array to study the expression of KHV genes in different conditions. Background to the topics-Mass mortality of koi or common carp has been observed in Israel, USA, Europe and Asia. These outbreaks have reduced exports of koi from Israel and have created fear about production, import, and movements of koi and have raised concerns about potential impacts on native cyprinid populations in the U.S.A. Major conclusions-A suite of new diagnostic tools was developed that included 3 PCR assays for detection of KHV DNA in cell culture and fish tissues and an ELISA assay capable of detecting anti-KHV antibodies in the serum of koi and common carp. The TKPCR assay developed during the grant has become an internationally accepted gold standard for detection of viral DNA. Additionally, the ELISA developed for detecting serum anti-KHV antibodies is now in wide use as a major nonlethal screening tool for evaluating virus status of koi and common carp populations. Real time PCR assays have been able to detect viral DNA in the internal organs of survivors of natural and wild type vaccine exposures at 1 and 10³ genome equivalents at 7 months after exposure. In addition, vaccinated fish were able to transmit the virus to naive fish. Potential control utilizing hybrids of goldfish and common carp for production demonstrated they were considerably more resistant than pure common carp or koi to both KHV (CyHV-3). There was no evidence that goldfish or other tested endemic cyprinids species were susceptible to KHV. The complete genomic sequencing of 3 strains from Japan, the USA, and Israel revealed a 295 kbp genome containing a 22 kbp terminal direct repeat encoding clear gene homologs to other fish herpesviruses in the family Herpesviridae. The genome encodes156 unique protein-coding genes, eight of which are duplicated in the terminal repeat. Four to seven genes are fragmented and the loss of these genes may be associated with the high virulence of the virus. Viral gene expression was studies by a newly developed chip which has allowed verification of transcription of most all hypothetical genes (ORFs) as well as their kinetics. Implications, both scientific and agricultural- The results from this study have immediate application for the control and management of KHV. The proposal provides elements key to disease management with improved diagnostic tools. Studies on the ecology of the virus also provide insights into management of the virus at the farms that farmers will be able to apply immediately to reduce risks of infections. Lastly, critical issues that surround present procedures used to create “resistant fish” must be be resolved (e.g. carriers, risks, etc.). Currently stamping out may be effective in eradicating the disease. The emerging disease caused by KHV continues to spread. With the economic importance of koi and carp and the vast international movements of koi for the hobby, this disease has the potential for even further spread. The results from our studies form a critical component of a comprehensive program to curtail this emerging pathogen at the local, regional and international levels.
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Dawson, William O., and Moshe Bar-Joseph. Creating an Ally from an Adversary: Genetic Manipulation of Citrus Tristeza. United States Department of Agriculture, January 2004. http://dx.doi.org/10.32747/2004.7586540.bard.
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Citrus is one of the major agricultural crops common to Israel and the United States, important in terms of nutrition, foreign exchange, and employment. The economy of both citrus industries have been chronically plagued by diseases caused by Citrus tristeza virus (CTV). The short term solution until virus-resistant plants can be used is the use of mild strain cross-protection. We are custom designing "ideal" protecting viruses to immunize trees against severe isolates of CTV by purposely inoculating existing endangered trees and new plantings to be propagated as infected (protected) citrus budwood. We crossed the substantial technological hurdles necessary to accomplish this task which included developing an infectious cDNA clone which allows in vitro manipulation of the virus and methods to then infect citrus plants. We created a series of hybrids between decline-inducing and mild CTV strains, tested them in protoplasts, and are amplifying them to inoculate citrus trees for evaluation and mapping of disease determinants. We also extended this developed technology to begin engineering transient expression vectors based on CTV as tools for genetic improvement of tree crops, in this case citrus. Because of the long periods between genetic transformation and the ultimate assay of mature tree characteristics, there is a great need for an effective system that allows the expression or suppression of target genes in fruiting plants. Virus-based vectors will greatly expedite progress in citrus genetic improvement. We characterized several components of the virus that provides necessary information for designing virus-based vectors. We characterized the requirements of the 3 ’-nontranslated replication promoter and two 3 ’-ORF subgenomic (sg) mRNA controller elements. We discovered a novel type of 5’-terminal sgRNAs and characterized the cis-acting control element that also functions as a strong promoter of a 3 ’-sgRNA. We showed that the p23 gene controls negative-stranded RNA synthesis and expression of 3 ’ genes. We identified which genes are required for infection of plants, which are host range determinants, and which are not needed for plant infection. We continued the characterization of native dRNA populations and showed the presence of five different classes including class III dRNAs that consists of infectious and self-replicating molecules and class V dRNAs that contain all of the 3 ’ ORFs, along with class IV dRNAs that retain non-contiguous internal sequences. We have constructed and tested in protoplasts a series of expression vectors that will be described in this proposal.
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Library, Spring. How Close are We Really to the HIV Cure? Spring Library, January 2020. http://dx.doi.org/10.47496/sl.blog.20.
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The virus’ tricky, constantly mutating nature has so far made it impossible to develop an effective vaccine, even as the constantly improving antiviral drug classes have made HIV infection a manageable chronic health condition.
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Semaan, Dima, and Linda Scobie. Feasibility study for in vitro analysis of infectious foodborne HEV. Food Standards Agency, September 2022. http://dx.doi.org/10.46756/sci.fsa.wfa626.
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Hepatitis E virus (HEV) is a member of the Hepeviridae family capable of infecting humans producing a range of symptoms from mild disease to kidney failure. Epidemiological evidence suggests that hepatitis E genotype III and IV cases may be associated with the consumption of undercooked pork meat, offal and processed products such as sausages [1]. A study carried out by the Animal Health and Veterinary Laboratories Agency (AHVLA), found hepatitis E virus contamination in the UK pork production chain and that 10% of a small sample of retail pork sausages were contaminated with the virus [2]. Furthermore, studies have confirmed the presence of HEV in the food chain and the foodborne transmission of Hepatitis E virus to humans [reviewed in 5]. Likewise, Scottish shellfish at retail [6] have also been found positive for HEV viral nucleic acid and some preliminary studies indicate that the virus is also detectable in soft fruits (L Scobie; unpublished data). There are current misunderstandings in what this data represents, and these studies have raised further questions concerning the infectivity of the virus, the processing of these foods by industry and the cooking and/or preparation by caterers and consumers. There are significant gaps in the knowledge around viral infectivity, in particular the nature of the preparation of food matrices to isolate the virus, and also with respect to a consistent and suitable assay for confirming infectivity [1,3]. Currently, there is no suitable test for infectivity, and, in addition, we have no knowledge if specific food items would be detrimental to cells when assessing the presence of infectious virus in vitro. The FSA finalised a comprehensive critical review on the approaches to assess the infectivity of the HEV virus which is published [3] recommending that a cell culture based method should be developed for use with food. In order to proceed with the development of an infectivity culture method, there is a requirement to assess if food matrices are detrimental to cell culture cell survival. Other issues that may have affected the ability to develop a consistent method are the length of time the virally contaminated sample is exposed to the cells and the concentration of the virus present. In most cases, the sample is only exposed to the cells for around 1 hour and it has been shown that if the concentration is less that 1x103 copies then infection is not established [3,5,10,11].
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Valverde, Rodrigo A., Aviv Dombrovsky, and Noa Sela. Interactions between Bell pepper endornavirus and acute viruses in bell pepper and effect to the host. United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598166.bard.
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Based on the type of relationship with the host, plant viruses can be grouped as acute or persistent. Acute viruses are well studied and cause disease. In contrast, persistent viruses do not appear to affect the phenotype of the host. The genus Endornavirus contains persistent viruses that infect plants without causing visible symptoms. Infections by endornaviruses have been reported in many economically important crops, such as avocado, barley, common bean, melon, pepper, and rice. However, little is known about the effect they have on their plant hosts. The long term objective of the proposed project is to elucidate the nature of the symbiotic interaction between Bell pepper endornavirus (BPEV) and its host. The specific objectives include: a) to evaluate the phenotype and fruit yield of endornavirus-free and endornavirus-infected bell pepper near-isogenic lines under greenhouse conditions; b) to conduct gene expression studies using endornavirus-free and endornavirus-infected bell pepper near-isogenic lines; and c) to study the interactions between acute viruses, Cucumber mosaic virus Potato virus Y, Pepper yellow leaf curl virus, and Tobacco etch virus and Bell pepper endornavirus. It is likely that BPEV in bell pepper is in a mutualistic relationship with the plant and provide protection to unknown biotic or abiotic agents. Nevertheless, it is also possible that the endornavirus could interact synergistically with acute viruses and indirectly or directly cause harmful effects. In any case, the information that will be obtained with this investigation is relevant to BARD’s mission since it is related to the protection of plants against biotic stresses.
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DeJong, Jocelyn. A question of scale? The challenge of expanding the impact of non-governmental organisations' HIV/AIDS efforts in developing countries. Population Council, 2001. http://dx.doi.org/10.31899/hiv2001.1003.
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There are currently more than 36 million people living with HIV/AIDS globally, and in 1999 5.3 million individuals were newly infected with the virus. AIDS activities initiated by nongovernmental organizations (NGOs) have been highly influential on thinking and strategies found within the HIV/AIDS sector. Yet NGOs often experience particular difficulties in increasing the scale of their activities to reach larger numbers of people, to have an impact at levels higher than the community, and to address the broader social determinants of HIV/AIDS. Perceiving the urgent need for NGOs to expand the scale of their activities in the face of an escalating epidemic, Horizons and the International HIV/AIDS Alliance launched an initiative to examine the nature of the challenge to scale up in the context of HIV/AIDS internationally. This publication was prepared as part of this initiative and addresses the specific challenge of deliberately increasing the scale of HIV/AIDS prevention, care, and support programs in developing countries.
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Gelb, Jr., Jack, Yoram Weisman, Brian Ladman, and Rosie Meir. Identification of Avian Infectious Brochitis Virus Variant Serotypes and Subtypes by PCR Product Cycle Sequencing for the Rational Selection of Effective Vaccines. United States Department of Agriculture, December 2003. http://dx.doi.org/10.32747/2003.7586470.bard.
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Objectives 1. Determine the serotypic identities of 40 recent IBV isolates from commercial chickens raised in the USA and Israel. 2. Sequence all IBV field isolates using PCR product cycle sequencing and analyze their S 1 sequence to detennine their homology to other strains in the Genbank and EMBL databases. 3. Select vaccinal strains with the highest S 1 sequence homology to the field isolates and perform challenge of immunity studies in chickens in laboratory trials to detennine level of protection afforded by the vaccines. Background Infectious bronchitis (IB) is a common, economically important disease of the chicken. IB occurs as a respiratory form, associated with airsacculitis, condemnation, and mortality of meat-type broilers, a reproductive form responsible for egg production losses in layers and breeders, and a renal form causing high mortality in broilers and pullets. The causative agent is avian coronavirus infectious bronchitis virus (IBV). Replication of the virus' RNA genome is error-prone and mutations commonly result. A major target for mutation is the gene encoding the spike (S) envelope protein used by the virus to attach and infect the host cell. Mutations in the S gene result in antigenic changes that can lead to the emergence of variant serotypes. The S gene is able to tolerate numerous mutations without compromising the virus' ability to replicate and cause disease. An end result of the virus' "flexibility" is that many strains of IBV are capable of existing in nature. Once formed, new mutant strains, often referred to as variants, are soon subjected to immunological selection so that only the most antigenically novel variants survive in poultry populations. Many novel antigenic variant serotypes and genotypes have been isolated from commercial poultry flocks. Identification of the field isolates of IBV responsible for outbreaks is critical for selecting the appropriate strain(s) for vaccination. Reverse transcriptase polymerase chain reaction (RT-PCR) of the Sl subunit of the envelope spike glycoprotein gene has been a common method used to identify field strains, replacing other time-consuming or less precise tests. Two PCR approaches have been used for identification, restriction fragment length polymorphism (RFLP) and direct automated cycle sequence analysis of a diagnostically relevant hypervariab1e region were compared in our BARD research. Vaccination for IB, although practiced routinely in commercial flocks, is often not protective. Field isolates responsible for outbreaks may be unrelated to the strain(s) used in the vaccination program. However, vaccines may provide varying degrees of cross- protection vs. unrelated field strains so vaccination studies should be performed. Conclusions RFLP and S1 sequence analysis methods were successfully performed using the field isolates from the USA and Israel. Importantly, the S1 sequence analysis method enabled a direct comparison of the genotypes of the field strains by aligning them to sequences in public databases e.g. GenBank. Novel S1 gene sequences were identified in both USA and Israel IBVs but greater diversity was observed in the field isolates from the USA. One novel genotype, characterized in this project, Israel/720/99, is currently being considered for development as an inactivated vaccine. Vaccination with IBV strains in the US (Massachusetts, Arkansas, Delaware 072) or in Israel (Massachusetts, Holland strain) provided higher degrees of cross-protection vs. homologous than heterologous strain challenge. In many cases however, vaccination with two strains (only studies with US strains) produced reasonable cross-protection against heterologous field isolate challenge. Implications S1 sequence analysis provides numerical similarity values and phylogenetic information that can be useful, although by no means conclusive, in developing vaccine control strategies. Identification of many novel S1 genotypes of IBV in the USA is evidence that commercial flocks will be challenged today and in the future with strains unrelated to vaccines. In Israel, monitoring flocks for novel IBV field isolates should continue given the identification of Israel/720/99, and perhaps others in the future. Strains selected for vaccination of commercial flocks should induce cross- protection against unrelated genotypes. Using diverse genotypes for vaccination may result in immunity against unrelated field strains.
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Levantovych, Oksana. COVID 19 MEDIA COVERAGE: AN ANALYSIS OF HEORHII POCHEPTSOV’S VIEW. Ivan Franko National University of Lviv, February 2021. http://dx.doi.org/10.30970/vjo.2021.49.11061.
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The article analyses the peculiarities of the coverage of the covid pandemic in the Ukrainian media, the emphasis placed by the media in news, and how the online mode of modern life and social distancing affects the growth of media influence. Special attention is paid to the view of the famous publicist Heorhii Pocheptsov, who does not exclude the possibility that the coronavirus was invented intentionally to control millions of people around the world. Permanently, the world faces numerous challenges of different scales: economic, military, socio-political, environmental, epidemiological ones. In 2020, the largest and the most unexpected event, undoubtedly, was the deadly coronavirus pandemic, which spread from the small Chinese province of Wuhan to the whole world and already took more than one million people’s lives in less than a year. Thus, the media, that in the post-information society actually have an unprecedented impact on people, form a person’s perception of such challenges. As a result, our understanding of the pandemic is directly related to the information we consume from the media. In fact, from the very start of quarantine, the media space began to be captured by analytical materials in which experts from various fields tried to predict what the world would be like after the end of coronavirus. These experts were of two types: some claimed that irreversible changes would deepen the permanent economic and socio-political crisis, and by claiming that they intensified panic, while others argued that any crisis is a chance to restart and grow. The experts put different emphases covering the covid pandemic in the media, but it is important to pay attention to the analysis of the famous publicist, propaganda researcher – Heorhii Pocheptsov, who sees the coronavirus as a tool to influence millions of people. The pandemic will end sooner or later, but no matter whether the virus was artificially invented or not, the processes that have already been launched around the world cannot stop as if nothing had happened. But Heorhii Pocheptsov’s opinion about the possible artificial nature of the virus should make us more vigilant while consuming information from TVs or from the online media, as it is possible that this information might be a part of a great game that we were not warned about.
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Meir, Shimon, Michael Reid, Cai-Zhong Jiang, Amnon Lers, and Sonia Philosoph-Hadas. Molecular Studies of Postharvest Leaf and Flower Abscission. United States Department of Agriculture, 2005. http://dx.doi.org/10.32747/2005.7696523.bard.
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Original objectives: Understanding the regulation of abscission competence by exploring the nature and function of auxin-related gene expression changes in the leaf and pedicelAZs of tomato (as a model system), was the main goal of the previously submitted proposal. We proposed to achieve this goal by using microarray GeneChip analysis, to identify potential target genes for functional analysis by virus-induced gene silencing (VIGS). To increase the potential of accomplishing the objectives of the previously submitted proposal, we were asked by BARD to show feasibility for the use of these two modern techniques in our abscission system. Thus, the following new objectives were outlined for the one-year feasibility study: 1.to demonstrate the feasibility of the VIGS system in tomato to perform functional analysis of known abscission-related genes; 2. to demonstrate that by using microarray analysis we can identify target genes for further VIGS functional analysis. Background to the topic: It is a generally accepted model that auxin flux through the abscission zone (AZ) prevents organ abscission by rendering the AZ insensitive to ethylene. However, the molecular mechanisms responsible for acquisition of abscission competence and the way in which the auxin gradient modulates it are still unknown. Understanding this basic stage of the abscission process may provide us with future tools to control abscission for agricultural applications. Based on our previous study, performed to investigate the molecular changes occurring in leaf and stem AZs of MirabillisJalapaL., we have expanded our research to tomato, using genomic approaches that include modern techniques for gene discovery and functional gene characterization. In our one-year feasibility study, the US team has established a useful system for VIGS in tomato, using vectors based on the tobacco rattle virus (TRV), a Lcreporter gene for silencing (involved in regulation of anthocyanin biosynthesis), and the gene of interest. In parallel, the Israeli team has used the newly released Affymetrix Tomato GeneChip to measure gene expression in AZ and non-AZ tissues at various time points after flower removal, when increased sensitivity to ethylene is acquired prior to abscission (at 0-8 h), and during pedicelabscission (at 14 h). In addition, gene expression was measured in the pedicel AZ pretreated with the ethylene action inhibitor, 1-methylcyclopropene (1-MCP) before flower removal, to block any direct effects of ethylene. Major conclusions, solutions and achievements: 1) The feasibility study unequivocally established that VIGS is an ideal tool for testing the function of genes with putative roles in abscission; 2) The newly released Affymetrix Tomato GeneChip was found to be an excellent tool to identify AZ genes possibly involved in regulation and execution of abscission. The VIGS-based study allowed us to show that TAPG, a polygalacturonase specifically associated with the tomato AZ, is a key enzyme in the abscission process. Using the newly released Affymetrix Tomato GeneChip we have identified potential abscission regulatory genes as well as new AZ-specific genes, the expression of which was modified after flower removal. These include: members of the Aux/IAAgene family, ethylene signal transduction-related genes, early and late expressed transcription factors, genes which encode post-translational regulators whose expression was modified specifically in the AZ, and many additional novel AZ-specific genes which were previously not associated with abscission. This microarray analysis allowed us to select an initial set of target genes for further functional analysis by VIGS. Implications: Our success in achieving the two objectives of this feasibility study provides us with a solid basis for further research outlined in the original proposal. This will significantly increase the probability of success of a full 3-year project. Additionally, our feasibility study yielded highly innovative results, as they represent the first direct demonstration of the functional involvement of a TAPG in abscission, and the first microarray analysis of the abscission process. Using these approaches we could identify a large number of genes involved in abscission regulation, initiation and execution, and in auxin-ethylene cross-talk, which are of great importance, and could enable their potential functional analysis by VIGS.
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Meir, Shimon, Michael S. Reid, Cai-Zhong Jiang, Amnon Lers, and Sonia Philosoph-Hadas. Molecular Studies of Postharvest Leaf and Flower Senescence. United States Department of Agriculture, January 2011. http://dx.doi.org/10.32747/2011.7592657.bard.
Full textAbstract:
Original objectives: To understand the regulation of abscission by exploring the nature of changes of auxin-related gene expression in tomato (Lycopersicon esculatumMill) abscission zones (AZs) following organ removal, and by analyzing the function of these genes. Our specific goals were: 1) To complete the microarray analyses in tomato flower and leaf AZs, for identifying genes whose expression changes early in response to auxin depletion; 2) To examine, using virus-induced gene silencing (VIGS), the effect of silencing target genes on ethylene sensitivity and abscission competence of the leaf and flower AZs; 3) To isolate and characterize promoters from AZ-specific genes to be used in functional analysis; 4) To generate stable transgenic tomato plants with selected genes silenced with RNAi, under the control of an AZ-specific promoter, for further characterization of their abscission phenotypes. Background: Abscission, the separation of organs from the parent plant, results in postharvest quality loss in many ornamentals and other fresh produce. The process is initiated by changes in the auxin gradient across the AZ, and is triggered by ethylene. Although changes in gene expression have been correlated with the ethylene-mediated execution of abscission, there is almost no information on the initiation of the abscission process, as the AZ becomes sensitized to ethylene. The present project was focused on elucidating these early molecular regulatory events, in order to gain a better control of the abscission process for agricultural manipulations. Major conclusions, solutions, achievements: Microarray analyses, using the Affymetrix Tomato GeneChip®, revealed changes in expression, occurring early in abscission, of many genes with possible regulatory functions. These included a range of auxin- and ethylene-related transcription factors (TFs), other TFs that are transiently induced just after flower removal, and a set of novel AZ-specific genes. We also identified four different defense-related genes, including: Cysteine-type endopeptidase, α- DOX1, WIN2, and SDF2, that are newly-associated with the late stage of the abscission process. This supports the activation of different defense responses and strategies at the late abscission stages, which may enable efficient protection of the exposed tissue toward different environmental stresses. To facilitate functional studies we implemented an efficient VIGS system in tomato, and isolated two abscission-specific promoters (pTAPG1 and pTAPG4) for gene silencing in stable transformation. Using the VIGS system we could demonstrate the importance of TAPGs in abscission of tomato leaf petioles, and evaluated the importance of more than 45 genes in abscission. Among them we identified few critical genes involved in leaf and flower abscission. These included: PTRP-F1, PRP, TKN4, KNOTTED-like homeobox TF, KD1, and KNOX-like homeodomain protein genes, the silencing of which caused a striking retardation of pedicel abscission, and ERF1, ERF4, Clavata-like3 protein, Sucrose transporter protein, and IAA10 genes, the silencing of which delayed petiole abscission. The importance of PRPand KD1 genes in abscission was confirmed also by antisense–silencing using pTAPG4. Experiments testing the effects of RNAi silencing of few other genes are still in progress, The analysis of the microarray results of flower and leaf AZs allowed us to establish a clear sequence of events occurring during acquisition of tissue sensitivity to ethylene, and to confirm our hypothesis that acquisition of ethylene sensitivity in the AZ is associated with altered expression of auxin-regulated genes in both AZs. Implication, both scientific and agricultural: Our studies had provided new insights into the regulation of the abscission process, and shaded light on the molecular mechanisms that drive the acquisition of abscission competence in the AZ. We pointed out some critical genes involved in regulation of abscission, and further expanded our knowledge of auxin-ethylene cross talk during the abscission process. This permits the development of novel techniques for manipulating abscission, and thereby improving the postharvest performance of ornamentals and other crops.