Dissertations / Theses on the topic 'Native parasitoids'

The introduction of exotic species is one of the most important threats to biodiversity.This phenomenon may cause economic and environmental damage. To prevent these invasions there are institutions like EPPO. Nevertheless, the introduction of exotic pests is an increasing issue, difficult to control. Classic biological control, based on importation of natural enemies from the country of origin, has been successfully used for over 120 years, but it has also raised some criticism. My research work has focused on the study of the new associations occurring between indigenous parasitoids and three exotic pests introduced in Italy and Europe. The three target insects considered were: Cacyreus marshalli Butler (Lepidoptera: Lycaenidae), a pest of Geranium plants; Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), a plague of Castanea sp. and Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae). This ladybug has been introduced as a biological control agent, but since some years it considered as an invasive species. For C. marshalli I performed laboratory tests on acceptance and suitability of immature stages of this butterfly by Exorista larvarum (Diptera: Tachinidae) and Brachymeria tibialis (Hymenoptera: Chalcidicae). The experiments showed that these two parasitoids could be used to contain this pest. For D. kuriphilus I performed field samplings in an infested chestnut area, the samples were maintained in rearing chamber until gall wasp or parasitoids emergence. In the 3-year research many parasitoids of gall wasps were found; one of these, Torymus flavipes (Walker), was found in large number. For H. axyridis the research work included a first phase of field sampling, during which I searched indigenous parasitoids which had adapted to this new host; the only species found was Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae). Laboratory tests were performed on the wasp rearing, biology and capacity to contain H. axyridis.
L'introduzione di specie esotiche è una delle minacce più importanti per la biodiversità, tale fenomeno può causare danni economici e ambientali. Anche per controllare queste introduzioni sono state create apposite istituzioni come EPPO. Tuttavia, l'introduzione di insetti esotici nocivi è un problema crescente, difficile da contrastare. Il controllo biologico classico, basato sull’introduzione dei nemici naturali dal paese di origine, è stato utilizzato con successo per oltre 120 anni, ma ha anche sollevato alcune critiche. Il mio lavoro di ricerca è stato incentrato sullo studio delle nuove associazioni tra parassitoidi indigeni e tre insetti esotici introdotti in Italia e in Europa. I tre insetti studiati sono stati: Cacyreus marshalli Butler (Lepidoptera: Lycaenidae), nocivo per le piante di Geranium; Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), dannoso al castagno e Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), coccinellide introdotto come agente di controllo biologico, ma da alcuni anni considerato come invasivo. Per C. marshalli sono stati effettuati degli esperimenti di laboratorio sulla accettabilità e l’idoneità di questo lepidottero da parte dei parassitoidi Exorista larvarum (Diptera: Tachinidae) e Brachymeria tibialis (Hymenoptera: Chalcididae). Gli esperimenti hanno dimostrato come questi due parassitoidi possano essere impiegati per il contenimento di questa specie. Per D. kuriphilus sono stati effettuati dei campionamenti in un castagneto infestato, i campioni sono stati mantenuti in celle climatizzate fino allo sfarfallamento del galligeno o dei parassitoidi. In tre anni di ricerca sono stati ritrovati molti parassitoidi, uno dei quali, Torymus flavipes (Walker), è stato rinvenuto in elevato numero. Per H. axyridis le ricerche prevedevano una fase di campo, per ricercare eventuali parassitoidi adattatisi a questo nuovo ospite; l’unica specie ritrovata è stata Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae). Con questa specie sono stati effettuati test per comprendere la sua biologia e la sua capacità di contenimento di H. axyridis, oltre che per migliorare le tecniche di allevamento.

The introduction of exotic species is one of the most important threats to biodiversity.This phenomenon may cause economic and environmental damage. To prevent these invasions there are institutions like EPPO. Nevertheless, the introduction of exotic pests is an increasing issue, difficult to control. Classic biological control, based on importation of natural enemies from the country of origin, has been successfully used for over 120 years, but it has also raised some criticism. My research work has focused on the study of the new associations occurring between indigenous parasitoids and three exotic pests introduced in Italy and Europe. The three target insects considered were: Cacyreus marshalli Butler (Lepidoptera: Lycaenidae), a pest of Geranium plants; Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), a plague of Castanea sp. and Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae). This ladybug has been introduced as a biological control agent, but since some years it considered as an invasive species. For C. marshalli I performed laboratory tests on acceptance and suitability of immature stages of this butterfly by Exorista larvarum (Diptera: Tachinidae) and Brachymeria tibialis (Hymenoptera: Chalcidicae). The experiments showed that these two parasitoids could be used to contain this pest. For D. kuriphilus I performed field samplings in an infested chestnut area, the samples were maintained in rearing chamber until gall wasp or parasitoids emergence. In the 3-year research many parasitoids of gall wasps were found; one of these, Torymus flavipes (Walker), was found in large number. For H. axyridis the research work included a first phase of field sampling, during which I searched indigenous parasitoids which had adapted to this new host; the only species found was Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae). Laboratory tests were performed on the wasp rearing, biology and capacity to contain H. axyridis.
L'introduzione di specie esotiche è una delle minacce più importanti per la biodiversità, tale fenomeno può causare danni economici e ambientali. Anche per controllare queste introduzioni sono state create apposite istituzioni come EPPO. Tuttavia, l'introduzione di insetti esotici nocivi è un problema crescente, difficile da contrastare. Il controllo biologico classico, basato sull’introduzione dei nemici naturali dal paese di origine, è stato utilizzato con successo per oltre 120 anni, ma ha anche sollevato alcune critiche. Il mio lavoro di ricerca è stato incentrato sullo studio delle nuove associazioni tra parassitoidi indigeni e tre insetti esotici introdotti in Italia e in Europa. I tre insetti studiati sono stati: Cacyreus marshalli Butler (Lepidoptera: Lycaenidae), nocivo per le piante di Geranium; Dryocosmus kuriphilus Yasumatsu (Hymenoptera: Cynipidae), dannoso al castagno e Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae), coccinellide introdotto come agente di controllo biologico, ma da alcuni anni considerato come invasivo. Per C. marshalli sono stati effettuati degli esperimenti di laboratorio sulla accettabilità e l’idoneità di questo lepidottero da parte dei parassitoidi Exorista larvarum (Diptera: Tachinidae) e Brachymeria tibialis (Hymenoptera: Chalcididae). Gli esperimenti hanno dimostrato come questi due parassitoidi possano essere impiegati per il contenimento di questa specie. Per D. kuriphilus sono stati effettuati dei campionamenti in un castagneto infestato, i campioni sono stati mantenuti in celle climatizzate fino allo sfarfallamento del galligeno o dei parassitoidi. In tre anni di ricerca sono stati ritrovati molti parassitoidi, uno dei quali, Torymus flavipes (Walker), è stato rinvenuto in elevato numero. Per H. axyridis le ricerche prevedevano una fase di campo, per ricercare eventuali parassitoidi adattatisi a questo nuovo ospite; l’unica specie ritrovata è stata Dinocampus coccinellae (Schrank) (Hymenoptera: Braconidae). Con questa specie sono stati effettuati test per comprendere la sua biologia e la sua capacità di contenimento di H. axyridis, oltre che per migliorare le tecniche di allevamento.

Senna mexicana chapmanii (Fabaceae: Caesalpinoideae), an attractive and threatened species native to pine rocklands of southern Florida, is consumed by folivorous caterpillars of Sulfur butterflies (Lepidoptera: Pieridae). Caterpillars may be deterred or eaten by predators, but also very important are parasitoids, both flies and wasps. This study investigated the effects of plant density on Sulfur caterpillar numbers and rates of parasitization. Senna mexicana chapmanii plantations were established at agricultural and urban areas; both sites are adjacent to protected pine rockland areas. Sulfur butterfly immature stages were collected and reared to glean information regarding number of herbivores and rates of parasitization. Continuing this weekly monitoring protocol over the course of a year provided data to determine that higher plant density has an effect on levels of parisitization and is correlated with the number of herbivores. Elucidating these patterns has important implications understanding the factors that regulate interactions in this plant/herbivore/parasitoid system.

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The objective of this study was to evaluate, under controlled conditions, the biological parameters of Anastrepha fraterculus in fruits of Campomanesia xanthocarpa (Berg.), cherry of rio grande (Eugenia involucrata DC.) And apple (Malus domestica) Gala variety and parameters morphological, biological and parasitism capacity D. longicaudata in larvae of A. fraterculus in fruits of C. xanthocarpa (Berg.), guava (Feijoa sellowiana Berg.), strawberry guava (Psidium cattleianum), Apple ( Malus domestica) Gala variety and peach (Prunus persica cv. Chimarrita). Before starting the experiment, green fruit C. xanthocarpa and the cherry of rio grande, were protected with TNT fabric bags to prevent oviposition of native flies. For apples were used without fruit of insecticides, kept in cold storage. The fruit maturation period were collected 200, 200 and 100 fruits of, C. xanthocarpa, cherry of rio grande and apple and placed in cages to infestation by A. fraterculus. Then these fruits were weighed individually stored in plastic pots with vermiculite, covered with fabric type voile and maintained in a room at 25 ± 1 C, 70 ± 10% RH and 14 hours photophase. The obtained pupae were weighed and isolated until the emergence of adults. After emergence, 25 couples each host were separated, and these individual cage formed by transparent plastic cup of 250 ml, whose top was covered with tissue type voile. At the top of each cage was placed a “oviposition panel” 7 cm in diameter made from rubberized fabric type voile with black silicone containing distilled water inside for obtaining eggs. For tests with D. longicaudata, fruits were protected with fabric bags TNT, picked in their respective ripening periods and taken to the laboratory to be infested by A. fraterculus. For C. xanthocarpa and strawberry guava they were infested 20 fruits of each species by repetition, totaling six repetitions. For the guava were held six repetitions with ten fruits in apple four replications with 20 fruits and peach were six replicates of ten fruits each. After eight to ten days the fruits were exposed to parasitoids in individual cages containing two couples of D. longicaudata per fruit. After this period the fruits were weighed individually stored in cups containing vermiculite, covered with fabric like voile and maintained in a room at 25 ± 1 C, 70 ± 10% RH and 14 hours photophase. The obtained pupae were placed in separate culture plates and subject to emergency flies or parasitoids. After emergence, it was found the size and parasitoid development time and the sex ratio. The percentage of parasitism was calculated by formula: N°. parasitoids / (N° of parasitoids + N°. flies) x 100. In the experiment on the biology of A. fraterculus larvae that developed in apple had the longest period of egg-larva (18.37 days) and egg-adult period (34.74 days). To adult stage, it was observed that the insects created in the cherry of rio grande and apple showed higher oviposition period, since the insects coming from C. xanthocarpa posted the largest daily fertility, with a rate of oviposition 20.70 eggs per day. Thus we can conclude that A. fraterculus completed all its stages in the evaluated fruits, occurring a delay in larval development in apples as a result of low nutritional value of this fruit as A.fraterculus host. Parasitoids in the percentage of parasitism in C. xanthocarpa, guava, strawberry guava, apple and peach was 88.34%; 87.63%; 69.66%, 71.05% and 34.25% respectively, confirming the good performance of the parasitoid that even though under laboratory conditions females of D. longicaudata inexperienced with fruit, were able to locate and parasitize fly larvae -the fruit. The size of the parasitoids was higher in A. fraterculus larvae that developed in C. xanthocarpa and peach. The parasitoids coming from C. xanthocarpa larvae showed the lowest development time for males (17.96 days) and females (20.94 days). Have low sex ratio, found in D. longicaudata female, coming from larvae of A. fraterculus that developed in guava and strawberry guava, may have association with the high percentage of unviable pupae, obtained in these hosts
O objetivo do presente trabalho foi avaliar, em condições controladas, os parâmetros biológicos de Anastrepha fraterculus em frutos de Cereja do rio grande (Eugenia involucrata DC.), Guabiroba (Campomanesia xanthocarpa Berg.) e Maçã (Malus domestica variedade Gala), a capacidade de parasitismo, os parâmetros biológicos e morfológicos de D. longicaudata em larvas de A. fraterculus em frutos de guabiroba (C. xanthocarpa Berg.), araçá-vermelho (Psidium cattleianum), goiaba-serrana (Feijoa sellowiana Berg.), pêssego (Prunus persica cv. Chimarrita) e Maçã (Malus domestica variedade Gala). Antes do início do experimento, os frutos verdes de guabiroba e cereja do rio grande, foram protegidos com sacos de tecido TNT para evitar a oviposição de moscas nativas. Para as maçãs foram utilizados frutos sem resíduo de inseticidas, mantidos em câmara fria. Na época de maturação dos frutos foram coletados 200, 200 e 100 frutos, de guabiroba, cereja do rio grande e maçã e acondicionados em gaiolas para infestação por A. fraterculus. Em seguida esses frutos foram pesados, armazenados individualmente em potes plásticos com vermiculita, cobertos com tecido tipo voile e mantidos em sala climatizada a 25±1 C, 70±10% de UR e 14 horas de fotofase. As pupas obtidas foram pesadas e individualizadas até a emergência dos adultos. Após a emergência, foram separados 25 casais de cada hospedeiro, sendo esses individualizados em gaiola formada por copo plástico transparente de 250 mL, cuja parte superior foi coberta com tecido tipo voile. Na parte superior de cada gaiola foi colocado um “painel de oviposição” de 7 cm de diâmetro confeccionado com tecido tipo voile emborrachado com silicone preto contendo água destilada em seu interior para a obtenção de ovos. Para os testes com D. longicaudata os frutos foram protegidos com sacos de tecido TNT, colhidos nas suas respectivas épocas de maturação e levados para o laboratório para serem infestados por A. fraterculus. Para a guabiroba e araçá-vermelho foram infestados 20 frutos de cada espécie por repetição, totalizando seis repetições. Para a goiaba-serrana foram realizadas seis repetições com dez frutos, em maçã quatro repetições com 20 frutos e para o pêssego foram seis repetições com dez frutos cada. Após oito a dez dias os frutos foram expostos aos parasitoides em gaiolas individualizadas contendo dois casais de D. longicaudata por fruto. Após esse período os frutos foram armazenados individualmente em copos, contendo vermiculita, cobertos com tecido tipo voile e mantidos em sala climatizada a 25±1 C, 70±10% de UR e 14 horas de fotofase. As pupas obtidas foram individualizadas em placas de cultura e observadas até a emergência de moscas ou parasitoides. Após a emergência, era verificado o tamanho e o tempo de desenvolvimento do parasitoide e a razão sexual. A porcentagem de parasitismo foi calculada pela formula: n° de parasitoides / (n° de parasitoides + n° de moscas) x 100. No experimento sobre a biologia de A. fraterculus, as larvas que se desenvolveram na maçã apresentaram o maior período de ovolarva (18,37 dias) e período de ovo-adulto (34,74 dias). Para a fase adulta, foi observado que os insetos que criados em cereja do rio grande e maçã apresentaram maior período de oviposição, já os insetos oriundos da guabiroba apresentaram a maior fecundidade diária, com um ritmo de oviposição de 20,70 ovos por dia. Desta forma podemos concluir que A. fraterculus completou todas as suas fases nos frutos avaliados, ocorrendo um atraso no desenvolvimento larval em maçãs, resultado da baixa qualidade nutricional desse fruto como hospedeiro de A. fraterculus. Nos parasitoides a porcentagem de parasitismo em guabiroba, araçá-vermelho, goiaba-serrana, maçã e pêssego foi de 88,34%; 87,63%; 69,66%, 71,05% e 34,25% respectivamente, constatando a ótima performance do parasitoide que mesmo sendo em condições de laboratório as fêmeas de D. longicaudata sem experiência prévia com frutos, foram capazes de localizar e parasitar as larvas de mosca-dasfrutas. O tamanho dos parasitoides foi maior em larvas de A. fraterculus que se desenvolveram em guabiroba e pêssego. Os parasitoides oriundos de larvas da guabiroba apresentaram o menor tempo de desenvolvimento para machos (17,96 dias) e fêmeas (20,94 dias). Já a baixa razão sexual, encontrada em fêmeas de D. longicaudata, oriundas de larvas de A. fraterculus que se desenvolveram no araçá-vermelho e na goiaba-serrana, pode ter associação com a alta porcentagem de pupas inviáveis, obtida nesses hospedeiros

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Geometridae larvae and their parasitoids were surveyed in a understory at Universidade Federal de São Carlos, Campus São Carlos, SP. Larvae were colected among April 2009 to April 2010, with an entomological umbrella in 18 native species plants. It obtained 863 larvae including 46 species and 24 genera belong to majority the Ennominae. The Larentiinae and Sterrhinae subfamilies were also represented for Eois and Cyclophora, respectively. The Ennominae genus obtained were: Certima, Glena, Herbita, Hymenomima, Iridopsis, Ischnopteris, Isochromodes, Lomographa, Macaria, Melanolophia, Microgonia, Microxydia, Nematocampa, Oxydia, Patalene, Pero, Phyllodonta, Physocleora, Prochoerodes, Sabulodes, Thyrinteina and Trotopera. Macaria rigidata was the more abundant species which represented 31%. It recorded 174 parasitized larvae from which 337 parasitoids emerged. They are distributed among Braconidae, Ichneumonidae, Eulophidae (Hymenoptera) and Diptera, with Hymenoptera predominance. The obtained parasitoids were: Diolcogaster, Protapanteles, Glyptapanteles, Aleiodes, (Braconidae), Casinaria, Charops, Cryptophion, Diradops, Dusona, Jomine, Neotheronia, Podogaster, Polycyrtus, Mesochorinae (Ichneumonidae), Euplectrus (Eulophidae) and Tachinidae (Diptera). It recorded new parasitoids species of the genus: Diradops, Jomine and Neotheronia (Hymenoptera, Ichneumonidae). Geometridae larvae were present throughout the collection period and had increased occurrence at the end of rainy season.
Foi realizado um levantamento de larvas de Geometridae e de seus parasitoides em um subbosque localizado na Universidade Federal de São Carlos, Campus São Carlos, SP. As larvas foram coletadas, no período de abril de 2009 a abril de 2010, por meio de guarda-chuva entomológico em 18 espécies de plantas nativas. Foram coletadas 863 larvas incluindo 46 espécies e 24 gêneros pertencentes na maioria aos Ennominae. As subfamílias Larentiinae e Sterrhinae foram também representadas, respectivamente com os gêneros Eois e Cyclophora. Os gêneros de Ennominae obtidos foram: Certima, Glena, Herbita, Hymenomima, Iridopsis, Ischnopteris, Isochromodes, Lomographa, Macaria, Melanolophia, Microgonia, Microxydia, Nematocampa, Oxydia, Patalene, Pero, Phyllodonta, Physocleora, Prochoerodes, Sabulodes, Thyrinteina e Trotopera. A espécie mais abundante foi Macaria rigidata que representou 31%. Foram registradas 174 larvas parasitadas das quais emergiram 337 parasitoides distribuídos em Braconidae, Ichneumonidae, Eulophidae (Hymenoptera) e Diptera, com a predominância de Hymenoptera. Os parasitoides obtidos foram: Diolcogaster, Protapanteles, Glyptapanteles, Aleiodes, (Braconidae), Casinaria, Charops, Cryptophion, Diradops, Dusona, Jomine, Neotheronia, Podogaster, Polycyrtus, Mesochorinae (Ichneumonidae), Euplectrus (Eulophidae) e Tachinidae (Diptera). Foram registradas novas espécies de parasitoides dos gêneros: Diradops, Jomine e Neotheronia (Hymenoptera, Ichneumonidae). As larvas de Geometridae estiveram presentes em todo o período de coleta e apresentaram maior ocorrência no final do período chuvoso.

In the mid-1980s, a new biotype of Bemisia tabaci was introduced into the southern U.S. causing extensive damage to agricultural crops throughout the region. An augmentative biological control project was initiated using a native parasitoid, Eretmocerus eremicus to determine its efficacy against B. tabaci infesting cotton in the desert areas of California and Arizona. A series of experiments were conducted in 1992-1995: release rate studies in cages and open fields; parasitoid dispersal within fields to determine movement rates after point release; and experiments to determine the parasitoid-host spatial relationship. Cage release rate studies, demonstrated that rates of parasitism could be increased 61 to 79% in the highest release treatments, with reductions in whitefly densities of 80 to 100% relative to control treatments. Cotton yields in the high release treatments peaked at 2.5 bales/ac and were 2.6 to 4.2 times greater than in control treatments. Effective release rates were estimated to be equivalent to 770,000 to 1.1 million parasitoids/ha. Field releases equivalent to 7.9 million parasitoids/ha resulted in a peak rate of parasitism of 42% but there were no statistical differences in parasitism, whitefly densities, or cotton yield; relative to no-release plots. High levels of whitefly immigration from surrounding crops, and parasitoid dispersal from release plots, diluted the effects of release. In a second field release rate study, releases equivalent to 2.0 to 3.0 million parasitoids/ha increased levels of the percentage of discovered leaves to greater than 80%. Parasitoid dispersal was analyzed with mark-recapture experiments and data were fit to a diffusion model. One female wasp flew 82 m in one day though the majority of wasps flew a few meters or less. Estimated diffusion rates and median dispersal distances were 0.40 to 0.71 m²/min and 2.4 to 4.4 m/(4 to 8) days respectively. Analysis of dispersal data suggested that releases on 20 m centers would provide effective coverage within a field. Density independent parasitism was common at the spatial scale of leaves and plants. There was positive density dependence for the percentage of discovered leaves suggesting that parasitoids aggregate to high density patches of whitefly but fail to achieve high levels of parasitization possibly due to egg limitation or mutual interference.

Chez la guêpe parasitoïde Venturia canescens, des particules virales dépourvues d'ADN appelées VLP (pour "Virus-like Particules") sont produites spécifiquement dans les ovaires et tapissent le chorion des oeufs qui sont injectés dans la chenille hôte. Les VLP ont une fonction immunosuppressive pour l'hôte parasité et permettent ainsi la survie des oeufs du parasitoïde. Ces VLP résultent de l’intégration d’un nudivirus dans le génome de l’ancêtre de la guêpe, nudivirus qui a été ensuite domestiqué pour former des liposomes viraux capables de véhiculer dans l’hôte des protéines de virulence d'origine cellulaire. L’étude réalisée au cours de cette thèse a eu pour objet, d’une part, d'étudier les mécanismes de domestication virale qui ont conduit au virus symbiotique endogène actuel nommé VcENV (pour V. canescens endogenous nudivirus) et d’autre part, d'apporter des éléments de réponse sur le processus de morphogénèse et le mode d'action parasitaire des VLP
Viral particles devoid of DNA called VLPs (for Virus-Like Particles) are specifically produced in the ovaries of the parasitoid wasp Venturia canescens and line the chorion of the wasp’s eggs injected into the host caterpillar. VLPs are immunosuppressive and allow parasitoid eggs survival. These VLPs result from the integration of a nudivirus into the wasp ancestor genome, nudivirus which was then domesticated to form viral liposomes capable of carrying, into the host, virulence proteins of cellular origin. The aim of the study carried out during this thesis was, first, to analyze the viral domestication mechanisms that led to the current endogenous symbiotic virus called VcENV (for V. canescens endogenous nudivirus) and secondly to provide some answers on VLPs morphogenesis process and parasitic mode of action

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Conselho Nacional de Desenvolvimento Científico e Tecnológico
This study shows the diversity of species of tephritid flies, their parasitoids and hosts in the region of Viçosa, Minas Gerais, located in the Zona da Mata Mineira. McPhail traps containing hydrolyzed protein, were installed in orchards with diverse species of fruit trees and a nature reserve, the Atlantic forest, forest known as the Mata do Paraíso. In addition, collections were made periodically cultivated and wild fruits in the region. Were obtained in 21 species of tephritid collections: Ceratitis capitata (Wied.), Anastrepha bahiensis Lima, A. barbiellinii Lima, A. bezzii Lima, A. bistrigata Bezzi, A. dissimilis Stone, A. distincta Greene, A. fraterculus (Wied.), A. furcata Lima, A. grandis (Macquart), A. leptozona Hendel, A. manihoti Lima, A. minensis Lima, A. montei Lima, A. obliqua (Macquart), A. pseudoparallela (Loew), A. pickeli Lima, A. serpentina (Wied.) and A. sororcula Zucchi, and two new species of Anastrepha. Deposited at the Regional Museum of Entomology (UFVB) found five species of tephritid A. connexa Lima, A. consobrina (Loew), A. kuhlmanni Lima, A. turpiniae Stone and A. xanthochaeta Hendel. 39 species of wild and 22 cultivated plant families sampled, we obtained C. capitata, A. bezzii, A. fraterculus, A. grandis, A. obliqua and A. sororcula. Parasitoids were also collected: Braconidae - Doryctobracon areolatus (Szépligeti), D. brasiliensis (Szépligeti), Opius bellus Gahan, Utetes anastrephae (Viereck) Figitidae - Aganaspis pelleranoi (Brèthes) and Pteromalidae - Sycophila sp.1, Sycophila sp.2, Torymus sp. and Pteromalidae sp.3. A taxonomic key for identification of species collected in Viçosa region is presented. An analysis for the fauna of Tephritidae species collected in the region of Viçosa, Minas Gerais. Assessing the population dynamics of three species was also performed, taking into account the influence of temperature, relative humidity and rainfall in addition to the dominance of these survey sites. Through this study added 19 species of fruit flies in Viçosa, Minas Gerais, bringing to 32 species of tephritid in Minas Gerais. Anastrepha barbiellinii, A. connexa, A. consobrina, A. furcata, A. kuhlmanni, A. leptozona and A. xanthochaeta were found first for the state and two new species of Anastrepha findings. Anastrepha fraterculus was the most frequent species in the samples of infected hosts and first observed occurrence in Lauraceae, Solanaceae Siparunaceae and in Brazil. The association of A. obliqua with tangerine and Ponkan cambucá and A. fraterculus with sweet cherry and passion fruit was also first observed through this study. The four species of parasitoids the Pteromalidae family, Sycophila sp.1, Sycophila sp.2, Torymus sp and a fourth species of Pteromalidae not yet identified, are new records for the state, passing 12 species of parasitoids of fruit flies in Minas Gerais. The population fluctuation of Anastrepha fraterculus and Ceratitis capitata in the region of Viçosa was influenced by temperature. Anastrepha minensis population fluctuation was influenced by temperature and precipitation. Fruticultura/Campus and Fruticultura/Fundão were more similar environments for the presence and absence of species of fruit flies (43%) while the small farm the environment was less similarity in terms of presence and absence of species (31 %).
Este estudo apresenta a diversidade de espécies de tefritídeos, seus parasitóides e hospedeiros na região de Viçosa, Minas Gerais, localizada na Zona da Mata Mineira. Armadilhas do tipo McPhail, contendo proteína hidrolisada, foram instaladas em pomares com espécies diversificadas de fruteiras e em uma reserva natural, remanescente da Mata Atlântica, conhecida como Mata do Córrego do Paraíso. Além disso, foram feitas coletas periódicas de frutos cultivados e silvestres na região. Foram obtidas 21 espécies de tefritídeos nas coletas: Ceratitis capitata (Wied.), Anastrepha bahiensis Lima, A. barbiellinii Lima, A. bezzii Lima, A. bistrigata Bezzi, A. dissimilis Stone, A. distincta Greene, A. fraterculus (Wied.), A. furcata Lima, A. grandis (Macquart), A. leptozona Hendel, A. manihoti Lima, A. minensis Lima, A. montei Lima, A. obliqua (Macquart), A. pseudoparallela (Loew), A. pickeli Lima, A. serpentina (Wied.) e A. sororcula Zucchi, além de duas novas espécies de Anastrepha. Depositadas no Museu Regional de Entomologia (UFVB) foram encontradas cinco espécies de tefritídeos: A. connexa Lima, A. consobrina (Loew), A. kuhlmanni Lima, A. turpiniae Stone e A. xanthochaeta Hendel. Das 39 espécies silvestres e cultivadas de 22 famílias botânicas amostradas, obtiveram-se C. capitata, A. bezzii, A. fraterculus, A. grandis, A. obliqua e A. sororcula. Foram coletados também os parasitóides: Braconidae - Doryctobracon areolatus (Szépligeti), D. brasiliensis (Szépligeti), Opius bellus Gahan, Utetes anastrephae (Viereck); Figitidae - Aganaspis pelleranoi (Brèthes); e Pteromalidae - Sycophila sp.1, Sycophila sp.2, Torymus sp. e Pteromalidae sp.3. Uma chave taxonômica para a identificação das espécies coletadas na região de Viçosa é apresentada. Foi realizada uma análise faunística para as espécies de Tephritidae coletados na região de Viçosa, Minas Gerais. A avaliação da flutuação populacional de três espécies também foi realizada, levando em conta a influência da temperatura, umidade relativa e precipitação pluviométrica, além da dominância destas para as áreas amostradas. Através deste estudo foram acrescentadas 19 espécies de moscas-das-frutas em Viçosa, Minas Gerais, elevando assim para 32 espécies de tefritídeos em Minas Gerais. Anastrepha barbiellinii, A. connexa, A. consobrina, A. furcata, A. kuhlmanni, A. leptozona e A. xanthochaeta foram constatadas pela primeira vez para o estado e duas espécies novas de Anastrepha descobertas. Anastrepha fraterculus foi a espécie mais frequente nas amostras de hospedeiros infestados e observada pela primeira vez a sua ocorrência em Lauraceae, Siparunaceae e Solanaceae no Brasil. A associação de A. obliqua com tangerina Ponkan e cambucá e de A. fraterculus com acerola e maracujá-doce também foi observada pela primeira vez por meio deste estudo. As quatro espécies de parasitóides da família Pteromalidae, Sycophila sp.1, Sycophila sp.2, Torymus sp e uma quarta espécie de Pteromalidae ainda não identificada, são novas ocorrências para o estado, passando para 12 espécies de parasitóides de moscas-das- frutas em Minas Gerais. A flutuação populacional de Anastrepha fraterculus e Ceratitis capitata na região de Viçosa foi influenciada pela temperatura. Anastrepha minensis teve a flutuação populacional influenciada pela temperatura e precipitação. Fruticultura/Campus e Fruticultura/Fundão foram os ambientes mais similares quanto à presença e ausência de espécies de moscas-das- frutas (43%) enquanto o Sítio foi o ambiente com menor similaridade quanto à presença e ausência de espécies (31%).

Seasonal fluctuations, development and phylogeny of Dubas bug Ommatissus lybicus de Bergevin (Hemiptera: Tropiduchidae) and its native natural enemy Pseudoligosita babylonica Viggiani (Hymenoptera: Trichogrammatidae) were studied on date palm Phoenix dactylifera Linnaeus (Arecales: Arecaceae) in the Sultanate of Oman. P. babylonica emergence and 0. lybicus egg hatching were significantly influenced by temperature whereas relative humidity had no significant effect. P. babylonica developed successfully at temperatures between 22.3°C and 32.4°C with an optimum at 30.0°C whilst no development occurred at temperatures of 17.6,34.4 and 37.2°C. 0. lybicus eggs developed at temperatures between 17.6°C and 32.4°C with an optimum at 27.5°C, whereas no development occurred at 34.4 and 37.2°C. Linear regression estimated the lower thermal threshold (Tzower) and thermal constant (DD) whereas non-linear models (Briere l " and Briere 2nd equations) estimated TZowen upper (Tmax) and optimum thermal threshold (Topt). P. babylonica successfully parasitised 0. lybicus eggs of 1 to 42 days old with embryogenesis of 1.3% to 57.2%. There was a negative response of P. babylonica female density on parasitism %. P. babylonica showed arrhenotokous parthenogenesis where male progeny developed from unfertilised eggs and females from fertilised eggs. Three sex forms were recorded (female, normal kale and deformed male). Deformed males successfully mated with females and this resulted in the production of all three forms. The results of phylogenic analysis on samples of 0. lybicus and P. babylonica collected from four different geographical locations in Oman revealed 98.1-100% nucleotide similarity among 0. lybicus samples with 8 different genotypes of which two were dominant based on sequences of the Cytochrome Oxidase Subunit I (COl) and showed 85% nucleotide similarity with Hemiptera species. P. babylonica samples shared 96.8-100% nucleotide similarity among each other with 10 different genotypes based on sequences of Cytochrome B (CytB) and shared 86% nucleotide similarity with Achrysocharoides sp. Predictive models were developed and tested for P. babylonica emergence and 0. lybicus egg hatching and showed promise for increasing the efficiency of P. babylonica in suppressing 0. lybicus populations and suggested improved timings for the applications of non-persistent insecticides used in an rPM programme. VI.

The parasitosis in the tropical countries like Ecuador, are found in the different population groups of all ages, sexes, social conditions, ethnic groups, especially in those without hygienic conditions, pertaining to different geographic zones and the prevalence varies in agreement with the ecology, the human factors and the social economic characteristics. The fecal contamination on land and water where an adequate disposition of excretes, the surrounding conditions, the deficient living conditions, the rural life, the absence of bathrooms, the custom to not use shoes and to have contact with the water. The deficiency of hygienic education, contamination of foods and human migration all favor intestinal parasites. The parasitic plague in Ecuador and especially in the province of Imbabura is not very well known, this is because of the little attention and economical means that are given to an investigation. This investigation clearly points out the different concepts, definitions, consequences, treatments and prophylaxis that the community should take into account. This investigative work was done in the rural area of the Imbabura province for the following ethnic groups: Indigenous, Black, and Mestizo, with children younger than five years old and it is directed to the health personnel, and students It is estimated that in the whole world more than 20 million people are infected by the "himenolepsis" parasite. The Intestinal parasite has a direct relation with the Nutritional state of any given country, as well as the basic services, like water, collection of trash, elimination of excretes, illiteracy, bad family hygiene habits, and a healthy environment. This is how 40 thousand children die of hunger in the world daily, the difference here is an American child eats 500 times more than third world child. The lack of interest by the governments both international and national in health Programs in rural zones, impedes a solution being found. 50% of investigations are dedicated to the advancements in military, which also influences the lack of water, 3 billion people in the world lack drinkable water. There are many forms of exposure to the parasite, including, ground (garbage or trash left on floor) or contaminated water, contaminated food, biting insects, domestic or wild animals which have the parasite, another person, and their clothes or bed sheets. The most common of these being contaminated water. There are many reasons these parasites are found in higher numbers in third world countries. The lack of healthiness in the rural population increases the intestinal parasite in children under five years old, the lack of knowledge by part of the mothers on the consequences of the intestinal parasites. Bad hygiene habits that the mothers have as well as the children under five years old in not washing their hands before eating and after using the restroom add to the rapid spread of the parasite. The nutritional state of the children greatly increases exposure, mostly concerning food preparation. The presence of intestinal parasites in the children under five years old does the same damage with no importance to age, race, or sex. The bad form in which excretes are eliminated in the town also contributes to parasite spreading. To fight against further parasite spreading it is advised that a program of investigation is applied with many disciplinary teams including: Doctors, Epidemists, Microbiologists, Anthropologist, Nutritionists, and nurses to study and help find solution for the well-being of the areas that most need it. To also help, it is advised that Nutritionists, Nurses, Doctors, and people who have direct knowledge of what is health, capacitate the leaders of the community and citizens on the basic hygiene norms, including preparation and manipulation of foods, consequences and ways to prevent parasites. This would help slow the rapid spread of parasites among youth as well as adults. Have meetings teaching proper use of bathrooms both private and public in rural areas, to avoid massive parasite contamination. It is important to teach families that they should treat the water before drinking it because water has a direct link with parasite spreading. Demand that government pay more attention to health issues in rural communities.

The results presented in this dissertation allow to better understand the outbreak dynamics of the ACGW, as well as the importance of different chestnut cultivar susceptibility. Furthermore, studying the role of native parasitoids is crucial to assess the impact of T. sinensis on endemic ACGW natural enemies once the exotic parasitoid colonizes these areas, either naturally or by future introductions. As regards the WCSB two highly specific DNA-based diagnostic protocols were devised, showing a promising sensitivity in the detection of WCSB biological traces. In addition, the potential of HRM analysis for insect genotyping was highlighted.

Invasive insects are considered one of the most serious threats affecting forests today; however, surprisingly little research has addressed the impacts of invasive species establishment on native forest insect communities. Such information is lacking for even the most thoroughly studied invasive forest insect, the gypsy moth. Using gypsy moth as a case study, my thesis addresses the questions: What are the ecological impacts of an exotic forest insect upon its establishment in a new community of native species? Does the community shift after the invasive establishes, and if so, what are the drivers in this realignment? I used multivariate analysis to assess native caterpillar communities collected in forest stands with and without a history of gypsy moth outbreak. I found that gypsy moth outbreak history had no significant effects on native caterpillar communities; however, current gypsy moth abundance was related to shifts in the structure of late season caterpillar assemblages. These results suggest that gypsy moth may affect native caterpillar communities through short-term mechanisms but not through long-term ecological changes. I used quantitative food webs to investigate the effects of gypsy moth on native host-parasitoid webs from the same caterpillar communities, and found that food web structure was resilient to both gypsy moth outbreak history and current abundance. The gypsy moth shared few parasitoids with native species in my study sites, none of numerical significance, thus minimizing the opportunity for enemy-mediated indirect interactions. Finally, I conducted a greenhouse experiment and found that early spring feeding by forest tent caterpillar can indirectly influence gypsy moth susceptibility to its virus, demonstrating that the complex interactions that can occur between native and exotic species do not always benefit the invader. Overall, I argue that the establishment of the gypsy moth into North American forests will not cause major changes in native caterpillar communities.