Academic literature on the topic 'Nanobioconjugates'

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Journal articles on the topic "Nanobioconjugates"

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Cajigas, Sebastian, and Jahir Orozco. "Nanobioconjugates for Signal Amplification in Electrochemical Biosensing." Molecules 25, no. 15 (August 3, 2020): 3542. http://dx.doi.org/10.3390/molecules25153542.

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Nanobioconjugates are hybrid materials that result from the coalescence of biomolecules and nanomaterials. They have emerged as a strategy to amplify the signal response in the biosensor field with the potential to enhance the sensitivity and detection limits of analytical assays. This critical review collects a myriad of strategies for the development of nanobioconjugates based on the conjugation of proteins, antibodies, carbohydrates, and DNA/RNA with noble metals, quantum dots, carbon- and magnetic-based nanomaterials, polymers, and complexes. It first discusses nanobioconjugates assembly and characterization to focus on the strategies to amplify a biorecognition event in biosensing, including molecular-, enzymatic-, and electroactive complex-based approaches. It provides some examples, current challenges, and future perspectives of nanobioconjugates for the amplification of signals in electrochemical biosensing.
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Caizer, Costica, Isabela Simona Caizer, Roxana Racoviceanu, Claudia Geanina Watz, Marius Mioc, Cristina Adriana Dehelean, Tiberiu Bratu, and Codruța Soica. "Fe3O4-PAA–(HP-γ-CDs) Biocompatible Ferrimagnetic Nanoparticles for Increasing the Efficacy in Superparamagnetic Hyperthermia." Nanomaterials 12, no. 15 (July 27, 2022): 2577. http://dx.doi.org/10.3390/nano12152577.

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In this paper, we present the obtaining of Fe3O4-PAA–(HP-γ-CDs) ferrimagnetic nanobioconjugates (PAA: polyacrylic acid, HP-γ-CDs: hydroxypropyl gamma-cyclodextrins) in a hybrid core-shell biostructure (core: inorganic Fe3O4 nanoparticles, and shell: organic PAA–(HP-γ-CDs)) and their use in superparamagnetic hyperthermia without cellular toxicity and with increased efficacy for future alternative cancer therapy. In order to design the optimal experimental conditions for obtaining nanobioconjugates and then superparamagnetic hyperthermia (SPMHT), we used molecular docking simulation and computational assessment of the maximum specific loss power (SLP) that led to nanoparticles’ heating. The nanoparticles and nanobioconjugates obtained were studied and characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transformed-infrared spectroscopy (FT-IR), dynamic light scattering (DLS), and magnetic measurements (MMs). The cell viability of the nanoparticles and nanobioconjugates was assessed by means of the MTT assay using human immortalized keratinocytes (HaCaT) as an in vitro model. Superparamagnetic hyperthermia with nanoparticles and nanobioconjugates was obtained experimentally in a magnetic field of 15.92 kA/m and frequency of 312.2 kHz for the magnetic nanoparticle core with a (average) diameter of 15.8 nm, which resulted in the maximum hyperthermic effect that led to a temperature of ~42.5 °C necessary in the therapy of tumors in a short time so as not to affect healthy tissues. The biological screening of Fe3O4-PAA nanoparticles and PAA–(HP-γ-CDs) nanobioconjugates showed no cytotoxic effect on HaCaT cells for a time interval of 24 h, both under standard (37 °C) and hyperthermia conditions (42.5 °C). Thus, Fe3O4-PA–(HP-γ-CDs) ferrimagnetic nanobioconjugates can be used successfully in superparamagnetic hyperthermia without toxicity and with increased efficiency due to the small layer thickness of the PAA–(HP-γ-CDs) shell, which is suitable in this alternative therapeutic technique.
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Umemura, Kazuo, and Shizuma Sato. "Scanning Techniques for Nanobioconjugates of Carbon Nanotubes." Scanning 2018 (June 13, 2018): 1–19. http://dx.doi.org/10.1155/2018/6254692.

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Nanobioconjugates using carbon nanotubes (CNTs) are attractive and promising hybrid materials. Various biological applications using the CNT nanobioconjugates, for example, drug delivery systems and nanobiosensors, have been proposed by many authors. Scanning techniques such as scanning electron microscopy (SEM) and scanning probe microscopy (SPM) have advantages to characterize the CNT nanobioconjugates under various conditions, for example, isolated conjugates, conjugates in thin films, and conjugates in living cells. In this review article, almost 300 papers are categorized based on types of CNT applications, and various scanning data are introduced to illuminate merits of scanning techniques.
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Asmat, Shamoon, Qayyum Husain, and Mohd Shoeb Khan. "A polypyrrole–methyl anthranilate functionalized worm-like titanium dioxide nanocomposite as an innovative tool for immobilization of lipase: preparation, activity, stability and molecular docking investigations." New Journal of Chemistry 42, no. 1 (2018): 91–102. http://dx.doi.org/10.1039/c7nj02951a.

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Prasad, Puja, and Shalini Gupta. "Nanobioconjugates: Weapons against Antibacterial Resistance." ACS Applied Bio Materials 3, no. 12 (November 20, 2020): 8271–85. http://dx.doi.org/10.1021/acsabm.0c01107.

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Sée, Violaine, Paul Free, Yann Cesbron, Paula Nativo, Umbreen Shaheen, Daniel J. Rigden, David G. Spiller, et al. "Cathepsin L Digestion of Nanobioconjugates upon Endocytosis." ACS Nano 3, no. 9 (September 3, 2009): 2461–68. http://dx.doi.org/10.1021/nn9006994.

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Ramírez-Acosta, Carlos M., Javier Cifuentes, Maria Claudia Castellanos, Rodolfo José Moreno, Carolina Muñoz-Camargo, Juan C. Cruz, and Luis H. Reyes. "PH-Responsive, Cell-Penetrating, Core/Shell Magnetite/Silver Nanoparticles for the Delivery of Plasmids: Preparation, Characterization, and Preliminary In Vitro Evaluation." Pharmaceutics 12, no. 6 (June 17, 2020): 561. http://dx.doi.org/10.3390/pharmaceutics12060561.

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Over the past decade, gene therapies have attracted much attention for the development of treatments for various conditions, including cancer, neurodegenerative diseases, protein deficiencies, and autoimmune disorders. Despite the benefits of this approach, several challenges are yet to be solved to reach clinical implementation. Some of these challenges include low transfection rates, limited stability under physiological conditions, and low specificity towards the target cells. An avenue to overcome such issues is to deliver the therapies with the aid of potent cell-penetrating vectors. Non-viral vectors, such as nanostructured materials, have been successfully tested in drug and gene delivery. Here, we propose the development and in vitro evaluation of a nanostructured cell-penetrating vehicle based on core/shell, magnetite/silver nanoparticles. A subsequent conjugation of a pH-responsive polymer was used to assure that the vehicle can carry and release circular DNA. Additionally, the translocating peptide Buforin II was conjugated with the aid of a polyether amine polymer to facilitate translocation and endosome escape. The obtained nanobioconjugates (magnetite/silver-pDMAEMA-PEA-BUFII) were characterized by UV-Vis spectrophotometry, dynamic light scattering (DLS), thermogravimetric analysis (TGA), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope equipped with energy dispersive spectroscopy (SEM+EDS), and transmission electron microscopy (TEM). They were also encapsulated in lecithin liposomes to form magnetoliposomes. The cell viability of Vero cells in the presence of the nanobioconjugates was above 95% and declined to 80% for the magnetoliposomes. The hemolytic tendency of nanobioconjugates and magnetoliposomes was below 10%, while the platelet aggregation approached that of the negative control (i.e., 35%). Cytoplasm coverage values of about 50% for both Vero and neuroblastoma cells confirmed significant cell penetration. Pearson’s correlation coefficients for both cell lines allowed us to estimate 20–40% colocalization of the nanobioconjugates with lysotracker green, which implied high levels of endosomal escape. The developed vehicles were also capable of loading around 16% of the added DNA and releasing such cargo with 8% efficiency. The developed nanoplatform holds a significant promise to enable highly efficient gene therapies as it overcomes some of the major issues associated with their eventual translation to the pre-clinical and clinical scale.
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Cuellar, Monica, Javier Cifuentes, Jessica Perez, Alejandra Suarez-Arnedo, Julian Serna, Helena Groot, Carolina Muñoz-Camargo, and Juan Cruz. "Novel BUF2-magnetite nanobioconjugates with cell-penetrating abilities." International Journal of Nanomedicine Volume 13 (November 2018): 8087–94. http://dx.doi.org/10.2147/ijn.s188074.

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Giraldo, Kevin A., Juan Sebastian Bermudez, Carlos E. Torres, Luis H. Reyes, Johann F. Osma, and Juan C. Cruz. "Microfluidics for Multiphase Mixing and Liposomal Encapsulation of Nanobioconjugates: Passive vs. Acoustic Systems." Fluids 6, no. 9 (August 31, 2021): 309. http://dx.doi.org/10.3390/fluids6090309.

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One of the main routes to ensure that biomolecules or bioactive agents remain active as they are incorporated into products with applications in different industries is by their encapsulation. Liposomes are attractive platforms for encapsulation due to their ease of synthesis and manipulation and the potential to fuse with cell membranes when they are intended for drug delivery applications. We propose encapsulating our recently developed cell-penetrating nanobioconjugates based on magnetite interfaced with translocating proteins and peptides with the purpose of potentiating their cell internalization capabilities even further. To prepare the encapsulates (also known as magnetoliposomes (MLPs)), we introduced a low-cost microfluidic device equipped with a serpentine microchannel to favor the interaction between the liposomes and the nanobioconjugates. The encapsulation performance of the device, operated either passively or in the presence of ultrasound, was evaluated both in silico and experimentally. The in silico analysis was implemented through multiphysics simulations with the software COMSOL Multiphysics 5.5® (COMSOL Inc., Stockholm, Sweden) via both a Eulerian model and a transport of diluted species model. The encapsulation efficiency was determined experimentally, aided by spectrofluorimetry. Encapsulation efficiencies obtained experimentally and in silico approached 80% for the highest flow rate ratios (FRRs). Compared with the passive mixer, the in silico results of the device under acoustic waves led to higher discrepancies with respect to those obtained experimentally. This was attributed to the complexity of the process in such a situation. The obtained MLPs demonstrated successful encapsulation of the nanobioconjugates by both methods with a 36% reduction in size for the ones obtained in the presence of ultrasound. These findings suggest that the proposed serpentine micromixers are well suited to produce MLPs very efficiently and with homogeneous key physichochemical properties.
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Nazir, Samina, Tajammul Hussain, Salman Akbar Malik, and Ayesha Younus. "The Synthesis and Evaluation of Novel Lactate Dehydrogenase Nanobioconjugates." Current Nanoscience 8, no. 2 (March 1, 2012): 299–304. http://dx.doi.org/10.2174/157341312800167623.

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Dissertations / Theses on the topic "Nanobioconjugates"

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Almeida, Miguel Peixoto de. "Nanobioconjugates of Gold and Silver Nanoparticles for Biosensors." Doctoral thesis, 2019. https://repositorio-aberto.up.pt/handle/10216/118510.

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Almeida, Miguel Peixoto de. "Nanobioconjugates of Gold and Silver Nanoparticles for Biosensors." Tese, 2019. https://repositorio-aberto.up.pt/handle/10216/118510.

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Book chapters on the topic "Nanobioconjugates"

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Dutta, Ankit K., and Naba K. Dutta. "Chapter 11. Protein, Biomimetic Protein, and Designer Peptide-directed Synthesis of Metal Nanoparticles, Metal Nanoclusters and Nanobioconjugates, and Their Potential Applications." In Biomaterials Science Series, 306–66. Cambridge: Royal Society of Chemistry, 2022. http://dx.doi.org/10.1039/9781788012720-00306.

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Dutta, Ankit K., and Naba K. Dutta. "Chapter 11. Protein, Biomimetic Protein, and Designer Peptide-directed Synthesis of Metal Nanoparticles, Metal Nanoclusters and Nanobioconjugates, and Their Potential Applications." In Biomaterials Science Series, 306–66. Cambridge: Royal Society of Chemistry, 2022. http://dx.doi.org/10.1039/9781788012720-00306.

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Shanmugam, Haripriya, Swathika Nataraj, Oviya Govindaraj, and Tamilnayagan Thangavel. "Nanobioconjugates: Plants and microbes assisted synthesis, mechanistics of surface functionalization and their applications." In Comprehensive Analytical Chemistry. Elsevier, 2023. http://dx.doi.org/10.1016/bs.coac.2023.02.003.

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Chawla, Ruchi, Varsha Rani, Mohini Mishra, and Krishan Kumar. "Integrated Role of Nanotechnology and Pharmacogenetics in Diagnosis and Treatment of Diseases." In Pharmacogenetics [Working Title]. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.97643.

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“One size fits all” is an erroneous paradigm in drug delivery, due to side effects/adverse effects and variability observed in drug response. The variability is a result of geneotypic variations (variability in genomic constitution) which is studied in the branch of science called Pharmacogenomics. The variability in drug response is studied by multigene analysis or profiling of whole-genome single nucleotide polymorphism (SNP) and is recorded in terms of the pharmacokinetic (absorption, distribution, metabolism and elimination) and pharmacodynamic (drug-receptor interaction, immune response, etc.) response of the drug. Therefore, a foray into this research area can provide valuable information for designing of drug therapies, identifying disease etiology, therapeutic targets and biomarkers for application in treatment and diagnosis of diseases. Lately, with the integration of pharmacogenomics and nanotechnology, a new facade for the diagnosis and treatment of diseases has opened up, and the prescription pattern of drugs has moved to pharmacotyping (individualized dose and dosage-form adjusted therapy) using nanoplatforms like nanobioconjugates, nanotheranostics, etc.
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Conference papers on the topic "Nanobioconjugates"

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Ljubimova, Julia Y. "Abstract 191: Double blockade of interacting CK2 and EGFR pathways by tumor-targeting nanobioconjugates increases therapeutic efficacy against glioblastoma multiforme." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-191.

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Forero, Maria Ximena, Carlos David Suarez, Manu Forero-Shelton, Veronica Akle, Juan C. Cruz, and Carolina Munoz-Camargo. "Novel anticancer agents based on co-immobilization of Temozolomide and Hydroxyurea on Magnetite-Buforin II nanobioconjugates: efficacy study in 3D Glioblastoma spheroids." In 2021 IEEE 2nd International Congress of Biomedical Engineering and Bioengineering (CI-IB&BI). IEEE, 2021. http://dx.doi.org/10.1109/ci-ibbi54220.2021.9626121.

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Ding, Hui, Satoshi Inoue, Alexander V. Ljubimov, Rameshwar Patil, Jose Portilla-Arias, Bindu Konda, Keith L. Black, Eggehard Holler, and Julia Y. Ljubimova. "Abstract 4428: Inhibition of tumor vascular protein laminin-411 by nanobioconjugate for glioma treatment." In Proceedings: AACR 102nd Annual Meeting 2011‐‐ Apr 2‐6, 2011; Orlando, FL. American Association for Cancer Research, 2011. http://dx.doi.org/10.1158/1538-7445.am2011-4428.

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Inoue, S., H. Golseiz, R. Patil, H. Ding, S. Rudenkyy, E. Holler, KL Black, and JY Ljubimova. "Direct tumor targeting using nanobioconjugate with a combination of monoclonal antibodies for breast cancer treatment." In CTRC-AACR San Antonio Breast Cancer Symposium: 2008 Abstracts. American Association for Cancer Research, 2009. http://dx.doi.org/10.1158/0008-5472.sabcs-2124.

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Inoue, Satoshi, Hui Ding, Bindu Konda, Jose Portilla-Arias, Rameshwar Patil, Andreas Espinoza, Sergiy Rudenkyy, et al. "Abstract 3854: Newly designed nanobioconjugate for direct targeting and systemic treatment of HER2-positive breast cancer." In Proceedings: AACR 101st Annual Meeting 2010‐‐ Apr 17‐21, 2010; Washington, DC. American Association for Cancer Research, 2010. http://dx.doi.org/10.1158/1538-7445.am10-3854.

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Siddiqui, Imtiaz A., Dhruba J. Bharali, Minakshi Nihal, Vaqar M. Adhami, Rahime Jashari, Shaker A. Mousa, and Hasan Mukhtar. "Abstract 251: Aptamer conjugated prostate specific membrane antigen (PSMA) targeting EGCG nanobioconjugate for prostate cancer prevention and treatment." In Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA. American Association for Cancer Research, 2014. http://dx.doi.org/10.1158/1538-7445.am2014-251.

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Ljubimova, Julia Y. "Abstract 5938:In vivotargeting of laminin-411-β1 integrin-Notch signaling pathway using nanobioconjugate alters glioma microenvironment for effective treatment." In Proceedings: AACR Annual Meeting 2017; April 1-5, 2017; Washington, DC. American Association for Cancer Research, 2017. http://dx.doi.org/10.1158/1538-7445.am2017-5938.

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Siddiqui, Imtiaz A., Dhruba J. Bharali, Rahime Jashari, Vaqar M. Adhami, Shaker A. Mousa, and Hasan Mukhtar. "Abstract 5263: Prostate-specific membrane antigen (PSMA)-targeting nanobioconjugate-encapsulated green tea polyphenol EGCG for prostate cancer prevention and therapy." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-5263.

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Siddiqui, Imtiaz A., Vaqar M. Adhami, Islam Rady, and Hasan Mukhtar. "Abstract 3725: Pre-clinical evaluation of prostate specific membrane antigen (PSMA) nanobioconjugate encapsulating green tea polyphenol EGCG for prostate cancer." In Proceedings: AACR Annual Meeting 2018; April 14-18, 2018; Chicago, IL. American Association for Cancer Research, 2018. http://dx.doi.org/10.1158/1538-7445.am2018-3725.

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