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Academic literature on the topic 'Myo-inositol 1'

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Published: 6 September 2023

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Journal articles on the topic "Myo-inositol 1"

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Rodrigues, Marta V., Nuno Borges, Mafalda Henriques, Pedro Lamosa, Rita Ventura, Chantal Fernandes, Nuno Empadinhas, Christopher Maycock, Milton S. da Costa, and Helena Santos. "Bifunctional CTP:Inositol-1-Phosphate Cytidylyltransferase/CDP-Inositol:Inositol-1-Phosphate Transferase, the Key Enzyme for Di-myo-Inositol-Phosphate Synthesis in Several (Hyper)thermophiles." Journal of Bacteriology 189, no. 15 (May 25, 2007): 5405–12. http://dx.doi.org/10.1128/jb.00465-07.

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ABSTRACT The pathway for the synthesis of di-myo-inositol-phosphate (DIP) was recently elucidated on the basis of the detection of the relevant activities in cell extracts of Archaeoglobus fulgidus and structural characterization of products by nuclear magnetic resonance (NMR) (N. Borges, L. G. Gonçalves, M. V. Rodrigues, F. Siopa, R. Ventura, C. Maycock, P. Lamosa, and H. Santos, J. Bacteriol. 188:8128-8135, 2006). Here, a genomic approach was used to identify the genes involved in the synthesis of DIP. Cloning and expression in Escherichia coli of the putative genes for CTP:l-myo-inositol-1-phosphate cytidylyltransferase and DIPP (di-myo-inositol-1,3′-phosphate-1′-phosphate, a phosphorylated form of DIP) synthase from several (hyper)thermophiles (A. fulgidus, Pyrococcus furiosus, Thermococcus kodakaraensis, Aquifex aeolicus, and Rubrobacter xylanophilus) confirmed the presence of those activities in the gene products. The DIPP synthase activity was part of a bifunctional enzyme that catalyzed the condensation of CTP and l-myo-inositol-1-phosphate into CDP-l-myo-inositol, as well as the synthesis of DIPP from CDP-l-myo-inositol and l-myo-inositol-1-phosphate. The cytidylyltransferase was absolutely specific for CTP and l-myo-inositol-1-P; the DIPP synthase domain used only l-myo-inositol-1-phosphate as an alcohol acceptor, but CDP-glycerol, as well as CDP-l-myo-inositol and CDP-d-myo-inositol, were recognized as alcohol donors. Genome analysis showed homologous genes in all organisms known to accumulate DIP and for which genome sequences were available. In most cases, the two activities (l-myo-inositol-1-P cytidylyltransferase and DIPP synthase) were fused in a single gene product, but separate genes were predicted in Aeropyrum pernix, Thermotoga maritima, and Hyperthermus butylicus. Additionally, using l-myo-inositol-1-phosphate labeled on C-1 with carbon 13, the stereochemical configuration of all the metabolites involved in DIP synthesis was established by NMR analysis. The two inositol moieties in DIP had different stereochemical configurations, in contradiction of previous reports. The use of the designation di-myo-inositol-1,3′-phosphate is recommended to facilitate tracing individual carbon atoms through metabolic pathways.
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Stephens, L. R., R. R. Kay, and R. F. Irvine. "A myo-inositol d-3 hydroxykinase activity in Dictyostelium." Biochemical Journal 272, no. 1 (November 15, 1990): 201–10. http://dx.doi.org/10.1042/bj2720201.

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A soluble ATP-dependent enzyme which phosphorylates myo-inositol has been characterized in Dictyostelium. The myo-inositol kinase activity was partially purified from amoebae by chromatography on DEAE-Sepharose and phenyl-Sepharose columns. The product of both the partially purified activity and of a crude cytosolic fraction was myo-inositol 3-phosphate. The partially purified preparations of myo-inositol kinase (a) possessed a Km for myo-inositol of 120 microM (in the presence of 5 mM-ATP) and for ATP of 125 microM (in the presence of 1 microM-myo-inositol), (b) did not recognize allo-, epi-, muco-, neo-, scyllo-, 1 D-chiro or 1 L-chiro-inositol as substrates, (c) were competitively inhibited by three naturally occurring analogues of myo-inositol: 1 L-chiro-inositol (Ki 49.5 +/- 0.7 microM: the structural equivalent of myo-inositol, except that the D-3 hydroxy moiety is axial), D-3-deoxy-myo-inositol [Ki 103 +/- 1 microM: (-)-viburnitol], and sequoyitol (Ki 271 +/- 7 microM; unlike 1 L-chiro-inositol and D-3-deoxy-myo-inositol, this was a substrate for the kinase), and finally (d) were apparently non-competitively inhibited by myo-inositol 3-phosphate. The product of myo-inositol kinase could be detected in intact amoebae and was a substrate for the first in a series of inositol polyphosphate kinases present in Dictyostelium which ultimately yield myo-inositol hexakisphosphate. The activity of myo-inositol D-3-hydroxykinase in Dictyostelium lysates showed evidence of developmental regulation.
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Burtle, G. J., and R. T. Lovell. "Lack of Response of Channel Catfish (Ictalurus punctatus) to Dietary Myo-inositol." Canadian Journal of Fisheries and Aquatic Sciences 46, no. 2 (February 1, 1989): 218–22. http://dx.doi.org/10.1139/f89-030.

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Channel catfish (Ictalurus punctatus) fingerlings were fed for 28 wk in aquaria (28 ± 1 °C) on semipurified diets with supplemental myo-inositol (400 mg∙kg diet−1), without myo-inositol, and without myo-inositol but with succinylsulfathiazole to suppress intestinal bacteria synthesis. Omission of myo-inositol from the diet, with or without the antibiotic, did not reduce growth rate, produce overt signs of myo-inositol deficiency, or cause a decrease in tissue (muscle, liver, and brain) concentration of myo-inositol. No lipid accumulation occurred in liver or kidney when myoinositol was deleted from the diet. The only possible lipotropic effect of myo-inositol deficiency was a slightly higher (P < 0.07) amount of lipid in brain tissue. Myo-inositol synthesis by enzymes in liver and brain tissues was not affected by myo-inositol in the diet. Rates of myo-inositol synthesis were 39.8 and 67.3 μmol∙h−1∙g protein−1 for liver and brain, which are higher than synthesis rates reported in rodents (myo-inositol synthesis has not been measured in other fish). This study showed that de novo synthesis of myo-inositol by fingerling channel catfish was sufficient for normal growth and maintenance of tissue levels of myo-inositol and to prevent overt signs of myo-inositol deficiency when the vitamin was not included in the diet.
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Stephens, L. R., P. T. Hawkins, A. F. Stanley, T. Moore, D. R. Poyner, P. J. Morris, M. R. Hanley, R. R. Kay, and R. F. Irvine. "myo-inositol pentakisphosphates. Structure, biological occurrence and phosphorylation to myo-inositol hexakisphosphate." Biochemical Journal 275, no. 2 (April 15, 1991): 485–99. http://dx.doi.org/10.1042/bj2750485.

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1. Standard and high-performance anion-exchange-chromatographic techniques have been used to purify myo-[3H]inositol pentakisphosphates from various myo-[3H]inositol-prelabelled cells. Slime mould (Dictyostelium discoideum) contained 8 microM-myo-[3H]inositol 1,3,4,5,6-pentakisphosphate, 16 microM-myo-[3H]inositol 1,2,3,4,6-pentakisphosphate and 36 microM-D-myo-[3H]inositol 1,2,4,5,6-pentakisphosphate [calculated intracellular concentrations; Stephens & Irvine (1990) Nature (London) 346, 580-583]; germinating mung-bean (Phaseolus aureus) seedlings contained both D- and L-myo-[3H]inositol 1,2,4,5,6-pentakisphosphate (which was characterized by 31P and two-dimensional proton n.m.r.) and D- and/or L-myo-[3H]inositol 1,2,3,4,5-pentakisphosphate; HL60 cells contained myo-[3H]inositol 1,3,4,5,6-pentakisphosphate (in a 500-fold excess over the other species), myo-[3H]inositol 1,2,3,4,6-pentakisphosphate and D- and/or L-myo-[3H]inositol 1,2,4,5,6-pentakisphosphate; and NG-115-401L-C3 cells contained myo-[3H]inositol 1,3,4,5,6-pentakisphosphate (in a 100-fold excess over the other species), D- and/or L-myo-[3H]inositol 1,2,4,5,6-pentakisphosphate, myo-[3H]inositol 1,2,3,4,6-pentakisphosphate and D- and/or L-myo-[3H]inositol 1,2,3,4,5-pentakisphosphate. 2. Multiple soluble ATP-dependent myo-inositol pentakisphosphate kinase activities have been detected in slime mould, rat brain and germinating mung-bean seedling homogenates. In slime-mould cytosolic fractions, the three myo-inositol pentakisphosphates that were present in intact slime moulds could be phosphorylated to myo-[3H]inositol hexakisphosphate: the relative first-order rate constants for these reactions were, in the order listed above, 1:8:31 respectively (with first-order rate constants in the intact cell of 0.1, 0.8 and 3.1 s-1, assuming a cytosolic protein concentration of 50 mg/ml), and the Km values of the activities for their respective inositol phosphate substrates (in the presence of 5 mM-ATP) were 1.6 microM, 3.8 microM and 1.4 microM. At least two forms of myo-inositol pentakisphosphate kinase activity could be resolved from a slime-mould cytosolic fraction by both pharmacological and chromatographic criteria. Rat brain cytosol and a soluble fraction derived from germinating mung-bean seedlings could phosphorylate myo-inositol D/L-1,2,4,5,6-, D/L-1,2,3,4,5-, 1,2,3,4,6- and 1,3,4,5,6-pentakisphosphates to myo-inositol hexakisphosphate: the relative first-order rate constants were 57:27:77:1 respectively for brain cytosol (with first-order rate constants in the intact cell of 0.0041, 0.0019, 0.0056 and 0.000073 s-1 respectively, assuming a cytosolic protein concentration of 50 mg/ml) and 1:11:12:33 respectively for mung-bean cytosol (with first-order rate constants in a supernatant fraction with a protein concentration of 10 mg/ml of 0.0002, 0.0022, 0.0024 and 0.0066 s-1 respectively).
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Lamosa, Pedro, Lu�s G. Gon�alves, Marta V. Rodrigues, L�gia O. Martins, Neil D. H. Raven, and Helena Santos. "Occurrence of 1-Glyceryl-1-myo-Inosityl Phosphate in Hyperthermophiles." Applied and Environmental Microbiology 72, no. 9 (September 2006): 6169–73. http://dx.doi.org/10.1128/aem.00852-06.

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ABSTRACT The accumulation of compatible solutes was studied in the hyperthermophilic bacterium Aquifex pyrophilus as a function of the temperature and the NaCl concentration of the growth medium. Nuclear magnetic resonance analysis of cell extracts revealed the presence of α- and β-glutamate, di-mannosyl-di-myo-inositol phosphate, di-myo-inositol phosphate, and an additional compound here identified as 1-glyceryl-1-myo-inosityl phosphate. All solutes accumulated by A. pyrophilus are negatively charged at physiological pH. The intracellular levels of di-myo-inositol phosphate increased in response to supraoptimal growth temperature, while α- and β-glutamate accumulated in response to osmotic stress, especially at growth temperatures below the optimum. The newly discovered compound, 1-glyceryl-1-myo-inosityl phosphate, appears to play a double role in osmo- and thermoprotection, since its intracellular pool increased primarily in response to a combination of osmotic and heat stresses. This work also uncovered the nature of the unknown compound, previously detected in Archaeoglobus fulgidus (L. O. Martins et al., Appl. Environ. Microbiol. 63:896-902, 1997). The curious structural relationship between diglycerol phosphate (found only in Archaeoglobus species), di-myo-inositol phosphate (a canonical solute of hyperthermophiles), and the newly identified solute is highlighted. This is the first report on the occurrence of 1-glyceryl-1-myo-inosityl phosphate in living systems.
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Offer, J., J. C. Metcalfe, and G. A. Smith. "The uptake of 3H-labelled monodeoxyfluoro-myo-inositols into thymocytes and their incorporation into phospholipid in permeabilized cells." Biochemical Journal 291, no. 2 (April 15, 1993): 553–60. http://dx.doi.org/10.1042/bj2910553.

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Monodeoxyfluoro-myo-inositols were applied to electropermeabilized and intact thymocyte preparations to study their metabolism and uptake in order to investigate their suitability as potential inhibitors of phosphoinositide-mediated cellular responses. Only three of the monodeoxyfluoro-myo-inositols were incorporated into the phospholipids of thymocytes: 1D-3-deoxy-3-fluoro-myo-inositol, 5-deoxy-5-fluoro-myo-inositol and 1D-6-deoxy-6-fluoro-myo-inositol, all of which were weaker substrates for phosphatidylinositol synthase than was myo-inositol. The 3-, 5- and 6-fluoro analogues also behaved as competitive inhibitors, with K1 values of 350 +/- 5 microM, 350 +/- 5 microM and 2.9 +/- 2 mM respectively, compared with a Km for myo-inositol of 31 +/- 4 microM. When incubated with electropermeabilized thymocyte preparations, these three analogues of myo-inositol all formed phospholipids with chromatographic properties which corresponded to those of substituted phosphatidylinositol and phosphatidylinositol monophosphate. The uptake of myo-inositol and of the monodeoxyfluoro-myo-inositols into intact thymocytes was studied by a dual-label technique. All the monodeoxyfluoro-myo-inositols were taken up to some extent, but only 2-deoxy-2-fluoro-myo-inositol and 1D-3-deoxy-3-fluoro-myo-inositol were actively concentrated. The monodeoxyfluoro-myo-inositols were also assayed for their ability to inhibit the uptake of myo-inositol into cells. Both 2-deoxy-2-fluoro-myo-inositol and 1D-3-deoxy-3-fluoro-myo-inositol were effective inhibitors of myo-inositol uptake. Furthermore, 1D-1-deoxy-1-fluoro-myo-inositol, which was not taken up actively, was an effective inhibitor of myo-inositol uptake. The three effective inhibitors all showed Ki values of approximately 150 microM, close to the apparent Km for inositol uptake of 180 microM, and the 4-, 5- and 6-fluoro analogues had Ki values in excess of 10 mM.
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deSolms, S. Jane, Joseph P. Vacca, and Joel R. Huff. "The total synthesis of (±)-myo-inositol-1,3,4-trisphosphate, (±)-myo-inositol-2,4,5-trisphosphate and (±)-myo-inositol-1,3,4,5-tetrakisphosphate." Tetrahedron Letters 28, no. 39 (January 1987): 4503–6. http://dx.doi.org/10.1016/s0040-4039(00)96548-1.

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Biffen, M., and D. E. Hanke. "Reduction in the level of intracellular myo-inositol in cultured soybean (Glycine max) cells inhibits cell division." Biochemical Journal 265, no. 3 (February 1, 1990): 809–14. http://dx.doi.org/10.1042/bj2650809.

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Although myo-inositol is included in media for the successful growth of plant tissues, the actual requirement of most tissues, including soybean (Glycine max) callus in suspension culture, for myo-inositol has not been demonstrated. We have made use of deoxyglucose to reduce intracellular levels of myo-inositol. Deoxyglucose is phosphorylated to deoxyglucose 6-phosphate, which inhibits L-myo-inositol 1-phosphate synthase, an important enzyme in the synthesis of myo-inositol. Addition of deoxyglucose to the medium resulted in a decrease in the intracellular level of myo-inositol that corresponded with a decrease in cell division. Cell viability was not affected. When myo-inositol was added to cells along with deoxyglucose, cell division was restored, as were intracellular levels of myo-inositol. Addition of myo-inositol had no affect on the uptake or metabolism of deoxyglucose. From these results we propose that myo-inositol has a role in maintaining cell division in soybean callus tissue in suspension culture.
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Martin, K. L., and T. K. Smith. "The myo-inositol-1-phosphate synthase gene is essential in Trypanosoma brucei." Biochemical Society Transactions 33, no. 5 (October 26, 2005): 983–85. http://dx.doi.org/10.1042/bst0330983.

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The de novo synthesis of myo-inositol occurs via a two-step process: first, glucose 6-phosphate is converted into inositol 1-phosphate by an INO1 (myo-inositol-1-phosphate synthase; EC 5.5.1.4); then, it is dephosphorylated by an inositol monophosphatase. The myo-inositol can then be incorporated into PI (phosphatidylinositol), which is utilized in a variety of cellular functions, including the biosynthesis of GPI (glycosylphosphatidylinositol) anchors. A putative INO1 was identified in the Trypanosoma brucei genome database and, by recombinant expression in Escherichia coli, was shown to be a catalytically active INO1. To investigate the importance of INO1, we created a conditional knockout, which, under non-permissive conditions, showed that INO1 is an essential gene in bloodstream form T. brucei and that the de novo synthesized myo-inositol is used for the formation of PI and GPI anchors.
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SIU, TEVA, and GREGORY A. AHEARN. "Inositol Transport by Hepatopancreatic Brush-Border Membrane Vesicles of the Lobster Homarus Americanus." Journal of Experimental Biology 140, no. 1 (November 1, 1988): 107–21. http://dx.doi.org/10.1242/jeb.140.1.107.

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The mechanism of [3H]myo-inositol transport by the lobster hepatopancreas was examined using purified brush-border membrane vesicles. Transport was stimulated by a 100 mmoll−1 inward Na+ gradient, but other cation gradients were ineffective, suggesting a Na+-dependent transfer mechanism. The transport system was most efficient at pH7.0 (both sides), rather than in the presence of a pH gradient (pHin = 7.0; pHout = 5.5) or at bilaterally low pH (pHin = pHout = 5.5). The system was shown to be electrogenic in two different ways. First, myo-inositol uptake was stimulated by anions of increasing permeability (SCN− &gt; Cl− &gt; gluconate). Second, an outwardly directed, valinomycin-induced K+ diffusion potential (inside negative) enhanced uptake in comparison with vesicles lacking the ionophore. Myo-inositol was transported by a carrier mechanism with an apparent Kt of 0.79mmoll−1, a Jmax of 6.3pmolmg protein−1 s−1, and by apparent diffusion with a permeability coefficient of 5.92 pmolmg protein−1s−1 (mmolT1)−1. D-Glucose was a noncompetitive inhibitor of myo-inositol uptake, but myo-inositol did not significantly reduce the transport of D-[3H]glucose. Vesicles preloaded with myo-inositol trans-stimulated [3H]myo-inositol uptake, whereas those preloaded with D-glucose did not, suggesting that the inositol carrier did not transport D-glucose. It is proposed that myo-inositol does not share the glucose carrier, and that D-glucose may modulateinositol influx by binding to a ‘regulator’ site on the inositol carrier.
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Dissertations / Theses on the topic "Myo-inositol 1"

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Dai, Zhijie, and 戴志洁. "The role of sodium/myo-inositol cotransporter 1 and myo-inositol in osteogenesis and bone formation." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2009. http://hub.hku.hk/bib/B43783533.

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Dai, Zhijie. "The role of sodium/myo-inositol cotransporter 1 and myo-inositol in osteogenesis and bone formation." Click to view the E-thesis via HKUTO, 2009. http://sunzi.lib.hku.hk/hkuto/record/B43783533.

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Chau, Fung-ling Jenny. "Developmental and physiological consequences of sodium/myo-inositol co-transporter 1 deficiency." Click to view the E-thesis via HKUTO, 2005. http://sunzi.lib.hku.hk/hkuto/record/B3549606X.

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Ananieva-Stoyanova, Elitsa Antonova. "Identification and Functional Role of Myo-Inositol Polyphosphate 5-Phosphatase Protein Complexes." Diss., Virginia Tech, 2009. http://hdl.handle.net/10919/28028.

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To survive, an organism must constantly adjust its internal state to changes in the environment from which it receives signals. The signals set off a chain of events referred to signal transduction. Signal transduction systems are especially important in multicellular organisms, such as plants and animals, because of the need to coordinate the activities of hundreds to trillions of cells. Plants, in particular, have a special need for perceiving signals from their environment because of their static nature. As in the animal cell, the first steps in perception of a signal include signal interaction with a receptor, signal amplification through second messenger production, and signal termination through second messenger hydrolysis. Myo-inositol polyphosphate 5-phosphatases (5PTases) (EC 3.1.3.56) have unique signal terminating abilities toward the second messenger inositol trisphosphate (Ins (1,4,5)P3, InsP3). In Arabidopsis thaliana there are 15 members of the 5PTase family, the majority of which contain a single 5PTase catalytic domain. Four members of the Arabidopsis 5PTase family, however, have a unique protein domain structure, with additional N-terminal WD40 repeats that are implicated in protein-protein interactions. The research presented here focused on the identification of 5PTase interacting proteins and the characterization of their functional role in Arabidopsis. To accomplish this goal, I examined a 5PTase13-interacting protein, the sucrose (Suc) nonfermenting-1-related kinase, SnRK1.1, an important energy sensor that is highly conserved among eukaryotes. My identification of a 5PTase13:SnRK1.1 complex points to the novel interaction of this metabolic modulator and inositol signaling/metabolism. 5PTase13 , however, plays a regulatory role in other plant specific processes as well, since I also identified the Arabidopsis homolog (Atp80) of the human WDR48 (HsWDR48, Hsp80) as a novel protein interactor of 5PTase13. My results indicate that Atp80 is important for leaf emergence, development and senescence likely via a regulatory interaction with 5PTase13 and PINOID â binding protein (PBP1).
Ph. D.
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Le, Calvez Pierre-Benoit. "Synthesis of novel multisubstrate adducts : putative inhibitors of myo-inositol 1-phosphate synthase." Thesis, Queen's University Belfast, 2010. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.527923.

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Alves, Karla Shangela da Silva. "Estudo dos nÃveis salivares de mioinositol e quiroinositol em crianÃas saudÃveis e portadores de diabetes infanto- juvenil." Universidade Federal do CearÃ, 2012. http://www.teses.ufc.br/tde_busca/arquivo.php?codArquivo=7224.

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CoordenaÃÃo de AperfeiÃoamento de NÃvel Superior
A Diabetes mellitus à uma doenÃa de causa mÃltipla, ocorrendo quando hà falta de insulina ou quando a mesma nÃo atua de forma eficaz, causando um aumento da taxa de glicose no sangue (hiperglicemia). Ainda nÃo se sabe precisamente o mecanismo de aÃÃo da insulina, alguns trabalhos sugerem que pode ser possivelmente mediado atravÃs do fosfoglicano inositol (IPGs), cujas algumas formas foram identificadas como: mioinositol e D-quiroinositol. Hà estudos que relacionam a reduÃÃo da glicemia em indivÃduos diabÃticos com o aparecimento desses inositÃis nas secreÃÃes corpÃreas, embora ainda nÃo haja registro de identificaÃÃo dessas molÃculas na composiÃÃo salivar. O objetivo deste estudo foi determinar a relaÃÃo salivar do mioinositol e quiroinositol em crianÃas com diabetes tipo 1 e comparar a presenÃa e concentraÃÃo dessas substÃncias com um grupo de crianÃas sadias (nÃo diabÃticas). Um total de 45 (quarenta e cinco) voluntÃrios, 25 com diabetes tipo 1 descompensados e 20 sadios (nÃo diabÃticos), de ambos os sexos, com idades de 3 a 12 anos, foram selecionados e convidados a participar do estudo. Amostras de saliva foram coletadas e centrifugadas. Os sobrenadantes foram separados, liofilizados e purificados. Logo em seguida, foram analisados por cromatografia lÃquida de alta eficiÃncia (HPLC) para a identificaÃÃo do mioinositol e quiroinositol. A partir dessa anÃlise, foi observado uma menor concentraÃÃo de quiroinositol (p=0,001, Kruskal- Wallis ANOVA seguido por mÃtodo de Dunnâs) e uma maior da concentraÃÃo de mioinositol (p=0,001, Kruskal- Wallis ANOVA seguido por mÃtodo de Dunnâs) nas crianÃas afetadas em comparaÃÃo com as crianÃas saudÃveis. Os pacientes com diabetes tiveram a razÃo mio/quiroinositol maior que do grupo controle (p=0,001, Kruskal- Wallis ANOVA seguido por mÃtodo de Dunnâs) e apresentaram uma correlaÃÃo entre sua proporÃÃo o DM1(p= 0,001). O resultado desse estudo sugere que o mioinositol e o quiroinositol encontrado na saliva de crianÃas com DM1 podem influenciar no controle metabÃlico e desempenhar um papel de marcadores da DM1.
Diabetes mellitus is a disease of multiples causes that occurs either when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin it produces, causing a rise in blood glucose levels (hyperglycemia). It is not clear the action mechanism of insulin but it has been suggested that inositol phosphoglicans, such as myoinositol and D-chiro-inositol, can be important secondary messengers in insulin signal transduction. Although there are some studies linking a reduction in blood glucose levels in diabetic patients with the presence of these inositols in body secretions, there are not reports about the presence of these molecules in salivary composition. Thus, this study aimed to determinate the myoinositol and D-chiro-inositol salivary relation in children with type 1 diabetes and to compare the presence and concentration of these molecules with healthy children (non-diabetic). It has been selected and invited 45 volunteers of both sexes aged 3-12 years, 25 with decompensate type 1 diabetes and 20 healthy children. Saliva samples were collected and centrifuged. The supernatants were separated, purified and lyophilized. The identification of myoinositol and D-chiro-inositol were carried out by means of high-performance liquid chromatography (HPLC). The results showed that children with type 1 diabetes have a lower concentration of D-chiro-inositol and a higher concentration of myoinositol than healthy children. Consequently, the myo/chiro-inositol rate was higher in type 1 diabetes children and there is a correlation between the rate and type 1 diabetes incidence. In conclusion, our data suggests that myoinositol and chiroinositol found in the saliva of children with type 1 diabetes may influence in metabolic control and plays an important role as markers of type 1 diabetes.
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Sayer, Lloyd. "A novel approach towards the stereoselective synthesis of inositols and its application in the synthesis of biologically important molecules." Thesis, University of St Andrews, 2016. http://hdl.handle.net/10023/15658.

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Myo-inositol is ubiquitous in nature and is found at the structural core of a diverse range of biologically important derivatives, including phosphatidylinositols, inositol phosphates and mycothiol. The synthesis of myo-inositol derivatives is notoriously difficult due to the need to control both regio- and enantioselectivity. As a result, synthetic routes to derivatives of this type are often lengthy and low yielding. The first biosynthetic step in the production of all myo-inositol metabolites is the isomerisation of D-glucose 6- phosphate to L-myo-inositol 1-phosphate as mediated by L-myo-inositol 1-phosphate synthase (INO1). For the protozoan parasite Trypanosoma brucei, INO1 is essential for survival and its version of the enzyme (TbINO1) has a high turnover. This makes TbINO1 an attractive candidate for the biocatalytic production of L-myo-inositol 1- phosphate, and a potential starting point for drastically shortened syntheses of important myo-inositol derivatives. The production of L-myo-inositol 1-phosphate by TbINO1 has been optimised to achieve complete conversion in reaction conditions that facilitate product isolation. Due to problems with an in-batch process, the TbINO1 enzyme was immobilised and the process was transferred to a flow system. This has allowed for production of significant quantities of L-myo-inositol 1-phosphate with a high level of purity. L-myo-inositol 1- phosphate obtained from the flow system has been used to prepare mycothiol glycosylation acceptor, 1,2,4,5,6-penta-O-acetyl-D-myo-inositol, in a concise synthesis with a greatly improved yield over the literature.
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Chopera, Denis Rutendo. "Molecular characterization of XvlNO1, a myo-inositol 1-phosphate synthase gene from Xerophyta viscosa." Master's thesis, University of Cape Town, 2005. http://hdl.handle.net/11427/4249.

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Includes bibliographical references.
Myo-inositol I-phosphate synthase (INO 1) catalyses the conversion of glucose-6-phosphate to myo-inositol I-phosphate, which is subsequently dephosphorylated to myo-inositol. Myo-inositol is a precursor for a number of important metabolites that include membrane components, storage molecules, phytohormones and a variety of osmoprotectants. Xerophyta viscosa Baker (Family Velloziaceae) is a monocotyledonous angiosperm which has the ability to resume full physiological function after desiccation. The full-length cDNA for INO1 from X viscosa was isolated using the RACE technique.
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Duthu, Brigitte. "Phosphoranylation de polyols : nouvelle voie d'acces aux myo-inositol phosphates." Toulouse 3, 1988. http://www.theses.fr/1988TOU30129.

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Un procede original de syntheses de myo-inositol phosphates a ete mis au point permettant d'obtenir en une seule preparation plusieurs myo-inositol phosphates a la fois. On fait reagir le myo-inositol avec un dioxa-2,8 aza-5 phospha-1 bicyclo(3. 3. 0) octane. Quand la phosphoranylation est totale on obtient les myo-inositol mono, bis, tris, tetrakis phosphates
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Hegeman, Carla Elizabeth. "Isolation and Characterization of Soybean Genes Involved in Phytic Acid Metabolism: Phytase and 1-L-myo-Inositol-1-Phosphate Synthase." Diss., Virginia Tech, 1999. http://hdl.handle.net/10919/29906.

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The objective of this research was to isolate and characterize soybean genes involved in phytic acid metabolism for use in genetic engineering strategies to improve phosphorus utilization. A soybean phytase from germinated cotyledons was purified 28,000-fold to apparent homogeneity and was determined to be a glycosylated homodimer with 70 kD subunits. Soybean phytase preferred phytate as substrate (Km = 60 mM) and was capable of removing of all six phosphate groups from phytate. The pH and temperature optima for soybean phytase activity were 4.5 and 58*C, respectively. The N-terminus and four internal peptides from the purified soybean phytase were sequenced by Edman degradation. The amino acid sequence data were used to design degenerate oligonucleotide primers for PCR amplification of the soybean phytase coding sequence. A protein 547 amino acids in length was predicted from the 1641 bp coding sequence. The phytase protein showed significant similarity to plant purple acid phosphatases (PAPs) and contained the conserved metallo-phosphomonoesterase active site motif. The soybean phytase coding sequence was placed under the control of a constitutive 35S CaMV promoter in a soybean biolistic transformation vector and was introduced into "Williams 82" suspension culture cells by particle bombardment. Stably transformed cell suspension lines were recovered. DNA blot analysis demonstrated that the recombinant soybean phytase coding sequence had integrated into the genomes of two cell lines. Expression of the transgene was confirmed by RNA blot analysis. Phytase activity was three to four fold higher in these two lines compared to control non-transformed cultures. A soybean L-myo-insoitol-1-phosphate synthase (MIPS) cDNA was isolated from total RNA from developing seeds. The protein encoded by the soybean MIPS cDNA showed 87-91% homology to MIPS protein sequences from other plant species. RNA blot analysis of staged developing soybean seeds revealed that MIPS is transcribed early in the cotyledonary stage of development. Compared to other soybean tissues, MIPS expression levels were highest in developing seeds. DNA blot analysis demonstrated that multiple copies of the MIPS gene are present within the soybean genome.
Ph. D.
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Books on the topic "Myo-inositol 1"

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Biswas, B. B., and Susweta Biswas, eds. myo-Inositol Phosphates, Phosphoinositides, and Signal Transduction. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0343-5.

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Healy, Christopher. Synthetic studies on the inhibition of L-myo-inositol 1-phosphate synthase. Birmingham: University of Birmingham, 1991.

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Tan, Chui Heong. Synthetic studies on the inhibition of L-myo-inositol-1-phosphate synthase. Birmingham: University of Birmingham, 1994.

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Book chapters on the topic "Myo-inositol 1"

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Schomburg, Dietmar, and Dörte Stephan. "myo-Inositol 1-kinase." In Enzyme Handbook 13, 913–15. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-59176-1_174.

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Schomburg, Dietmar, and Dörte Stephan. "myo-Inositol 1-O-methyltransferase." In Enzyme Handbook 11, 169–71. Berlin, Heidelberg: Springer Berlin Heidelberg, 1996. http://dx.doi.org/10.1007/978-3-642-61030-1_37.

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Schomburg, Dietmar, and Margit Salzmann. "1L-Myo-inositol-1-phosphatase." In Enzyme Handbook 3, 411–16. Berlin, Heidelberg: Springer Berlin Heidelberg, 1991. http://dx.doi.org/10.1007/978-3-642-76463-9_86.

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Schomburg, Dietmar, and Dörte Stephan. "1D-myo-Inositol-tetrakisphosphate 1-kinase." In Enzyme Handbook, 151–53. Berlin, Heidelberg: Springer Berlin Heidelberg, 1997. http://dx.doi.org/10.1007/978-3-642-59025-2_29.

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Murthy, Pushpalatha P. N. "Inositol Phosphates and Their Metabolism in Plants." In myo-Inositol Phosphates, Phosphoinositides, and Signal Transduction, 227–55. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0343-5_8.

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Raboy, Victor, and Paolo Gerbasi. "Genetics of myo-Inositol Phosphate Synthesis and Accumulation." In myo-Inositol Phosphates, Phosphoinositides, and Signal Transduction, 257–85. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0343-5_9.

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Sopory, Sudhir K., and Meena R. Chandok. "Light-Induced Signal Transduction Pathway Involving Inositol Phosphates." In myo-Inositol Phosphates, Phosphoinositides, and Signal Transduction, 345–70. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0343-5_12.

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Schultz, Carsten, Anne Burmester, and Christoph Stadler. "Synthesis, Separation, and Identification of Different Inositol Phosphates." In myo-Inositol Phosphates, Phosphoinositides, and Signal Transduction, 371–413. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0343-5_13.

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Hokin, Lowell E. "History of Phosphoinositide Research." In myo-Inositol Phosphates, Phosphoinositides, and Signal Transduction, 1–41. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0343-5_1.

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Biswas, Susweta, and B. B. Biswas. "Metabolism of myo-Inositol Phosphates and the Alternative Pathway in Generation of myo-Inositol Trisphosphate Involved in Calcium Mobilization in Plants." In myo-Inositol Phosphates, Phosphoinositides, and Signal Transduction, 287–316. Boston, MA: Springer US, 1996. http://dx.doi.org/10.1007/978-1-4613-0343-5_10.

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Conference papers on the topic "Myo-inositol 1"

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Sajja, Sujith, Shane A. Perrine, Farhad Ghoddoussi, Matthew P. Galloway, and Pamela J. VandeVord. "Increased Levels of Myo-Inositol are Associated With Impaired Working Memory and Active Avoidance in Blast Neurotrauma Animals." In ASME 2012 Summer Bioengineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/sbc2012-80466.

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Impaired working memory and anxiety are major clinical symptoms commonly associated with subjects exposed to blast overpressure [1–4]. Despite this association, there remains a vital need for biomarkers to help effectively diagnosis blast-induced neurotrauma (BINT). Clinically, elevated myo-inositol has been associated with several neurodegenerative disorders including dementia and elevated levels may reflect activation of microglia. In the present study, we evaluated the cognitive and behavioral changes in blast exposed animals using the novel object recognition (working memory paradigm) and light/dark (anxiety test) assessments. In addition, we used high resolution magic angle spinning H-MRS to assess neurochemical changes in the prefrontal cortex and amygdala, brain regions associated with working memory and anxiety respectively. Results suggest that exposure to blast has a significant effect on the levels of myo-inositol which appear to be linked with impaired working memory.
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Medini, L., P. Maderna, E. Tremoli, and C. Galli. "PLATELETS FROM TYPE IIA HYPERCHOLESTEROLEMIC PATIENTS GENERATE MORE INOSITOLPHOSPHATES AFTER THROMBIN STIMULATION IN COMPARISON WITH THOSE OF NORMAL SUBJECTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643415.

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Functional and biochemical responses of platelets to stimulating agents have been reported to be amplified in several pathological conditions, including hyperlipidemia. Enhanced aggregation and thromboxane formation are, e.g. frequently observed in the presence of high plasma cholesterol levels (type Ila hypercholesterolemia). Stimulation of phosphoinositides breakdown through specific phosphohydrolases (phospholipase C) resulting in the formation of inositolphosphates (IPs), is one of the early events in platelet activation. A study was thus designed in order to investigate IPs generation in thrombin stimulated platelets from type Ila hypercholesterolemic patients in comparison with a group of normocholesterolemic subjects. Preparation of washed platelets, prelabelling with 2[3H] myo inositol and separation of lipid and water soluble inositides was carried out according to Watson et al (1). Product generation (inositoltrisphosphate, IP3; inositolbisphosphate, IP2; inositol mono phosphate, IP) in relation to the 2[3H) inositol incorporated in phosphoinositides was evaluated at 10 and 90 s after platelet stimulation with 1 U/ml NIH in the presence of 10 mM lithium chloride. The major differences found in platelets from type Ila patients in comparison with those of controls were the following:1) in non stimulated platelets a greater incorporation of myoinositol in phosphoinositides and lower levels of labelled IP2; 2) after stimulation, the levels of all labelled IPs were significantly greater, and those of IP2 were double than in controls. In particular the percent increment of IP2 over basal values in platelets of type Ila patients was more than two fold greater. It is concluded that the enhanced generation of IPs in platelets from type IIa patients, following thrombin stimulation, may contribute to the greater sensitivity to agonists of the aggregatory process in this pathological condition.1) Watson P.S., McConnell R.T. and Lapetina E.G., J. Biol. Chem.21:13199, 1984
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Novais, Aurea Maria Lago, and Renan Carvalho Castello Branco. "Mechanisms of Neuroplasticity After Pediatric Stroke: A Review." In XIII Congresso Paulista de Neurologia. Zeppelini Editorial e Comunicação, 2021. http://dx.doi.org/10.5327/1516-3180.241.

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Introduction: Stroke in childhood constitute a rare event and its incidence is increasing due to advances in neuroimaging.This study clarifies anatomic and molecular mechanisms involved in neuroplasticity after children stroke, demonstrating its specificities in motor,somatosensory and language habilities. Methods: We used database, from 2000 to march 2021,of SpringerLink,NEJM,PubMed, AHA (Stroke),Scielo,VHL and JAMA.The research was based in the keywords “neuplasticity”, “stroke” and “children”; 57 were selected including original articles, case reports and reviews, considering abstract according to the objective of the present study and methodologies that satisfy criterias of cientific valuation, considering p <0,005 as statistical significance. Results: Reduction of ipsilesional cortex and better prognosis between the ages of 1 and 6 years were observed. About motor function, it was found persistence of some perilesionais circuits, contralateral reorganization with increasing activation of suplementary motor area, unbalance of intrahemisferics inhibitory mechanisms, increase of excitability and changes in the concentration of N-acetyl-aspartate, choline, myo-inositol and creatine. Somatosensory skills presented limited plasticity. Contralesional alterations in arched fasciculi and temporoparietal area, circuit remodelation and compromissing of complex cognitive functions were observed for language habilites. Conclusion: Better outcomes in the ages of 1 to 6 years demonstrate the duality between early vulnerability and early plasticity. The plasticity of motor system demonstrates therapeutic targets and potencial rehabilitation markers; otherwise, the limited potencial of somatossensorial habilities indicates its premature determination. Language skills presented limited prognosis.
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