Dissertations / Theses on the topic 'Murine mode'
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Pileyre, Baptiste. "Etude préclinique évaluant l'utilisation de la fraction vasculaire stromale et les cellules souches dérivées de tissu adipeux dans le traitement des myosites." Electronic Thesis or Diss., Normandie, 2023. http://www.theses.fr/2023NORMR054.
Myositis is a group of autoimmune muscular diseases affecting skeletal muscles, leading to severe damage. While most patients respond to conventional treatments, some remain refractory. The stromal vascular fraction (SVF), freshly extracted from adipose tissue, or stem cells derived from it (ADSC) by expansive culture have shown promising results in various autoimmune pathologies and could enable the management of these patients. To assess their potential and study the mechanisms of action of these therapies, we carried out preclinical studies in vitro and in vivo. To this end, we characterized and used a mouse model of myositis, the Icos-/- NOD mouse, showing spontaneous muscle damage associated with extensive leukocyte infiltrates. To be closer of the autologous use of these therapies, we performed syngeneic transplants of FVS and ADSC in this model. We observed a slowdown in the progression of symptoms, a reduction in the loss of muscle strength and a decrease in muscle atrophy in vivo. We have demonstrated a greater efficacy of ADSCs, particularly in in vitro assays. These tests, carried out with donor cells and assessing regenerative efficacy (fibroblast colony forming unit assay) and immunomodulatory effect (lymphocyte proliferation inhibition assay), demonstrated the dose-dependent effect of these therapies, as well as the superiority of ADSCs in terms of both maximum efficacy and 50% inhibitory dose of the immunomodulatory effect. They also enabled us to identify markers predictive of the proliferative (CD90, CD105 or high hematopoietic stem cell count) and immunomodulatory (CD73 or macrophage count) effects of these therapies. All these elements demonstrate the potential of these therapies in the treatment of myositis, and the interest of investigating their effect through clinical trials
Castelló, Carla Martí. "Ultrassonografia do tumor sólido de Ehrlich inoculado em camundongos." Universidade Federal de Goiás, 2017. http://repositorio.bc.ufg.br/tede/handle/tede/7130.
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The research to prevent cancer, diagnose early, and find new therapies is one of the main challenges of current medicine, and in vivo tumor models are essential for this aim. Imaging techniques, such as ultrasound, assists the research by helping to obtain data that are more accurate and to reduce the number of animals necessary to obtain statistically significant results. Ehrlich carcinoma is one of the most widely used models but it has not an ultrasonographic description. In this study, serial ultrasound examinations were performed, in B-mode and Doppler, on Ehrlich solid carcinomas (ESC) inoculated in mice. From the measurements obtained by ultrasound, the growth patterns were analyzed and the tumors were separated in two groups depending on the specific growth rate (SGR). Ultrasonographic characteristics of capsule, margins, echotexture, vascular flow, and Doppler indices of Resistivity Index (RI) and Pulsability Index (PI) were compared between groups. ESC presents variable growth patterns; a capsule detectable by ultrasound, which sometimes present discontinuity; detectable flow in most of the exams; and the possibility of a central focus of necrosis or several necrosis focuses separated by tissue. In conclusion, tumors with high vascularization tend to have high SGR, while tumors that presented homogeneous echotexture and absence of blood flow tend to have lower SGR. The study also showed that changes in tumor vessels are reflected in Doppler indices, with significantly lower RI and PI, than normal vessels
A pesquisa para o diagnóstico precoce, prevenção e novos tratamentos do câncer é um dos principais desafios da medicina atual e os modelos tumorais in vivo são imprescindíveis para esse fim. Técnicas de imagem, como a ultrassonografia, auxiliam a obter dados mais precisos e diminuir o número de animais para obter resultados estatisticamente significativos. O carcinoma de Ehrlich é um dos modelos mais usados, mas não existem descrições ultrassonográficas dele. Nesse trabalho, foram realizados exames ultrassonográficos seriados, em modo-B e Doppler, em carcinomas sólidos de Ehrlich (ESC) inoculados em camundongos. A partir das medidas obtidas por ultrassom, foram analisados os padrões de crescimento e os tumores foram separados em dois grupos dependendo da taxa específica de crescimento (SGR). Foram comparadas as características ultrassonográficas da capsula, margens, ecotextura, distribuição e quantidade de fluxo e os índices Doppler, Índice de Resistividade (IR) e Índice de Pulsabilidade (IP), entre os grupos. Os ESCs apresentaram-se como tumores com padrões de crescimento variáveis; uma capsula detectável por ultrassonografia, mas que pode apresentar descontinuidade em alguns casos; fluxo detectável na maioria dos exames; e que podem apresentam um foco de necrose central ou vários focos de necrose separados. Conclui-se que os tumores que apresentam alta vascularização tendem a ter alta SGR enquanto os que apresentam ausência de fluxo e ecotextura homogênea estão correlacionados com baixa SGR. O estudo também demonstrou que as alterações e mudanças nos vasos tumorais se encontram refletidas nos índices Doppler, apresentando IR e IP significativamente menores as dos vasos extratumorais.
Zgheib, Sara. "Altérations physiologiques et récupération à long terme dans un modéle murin de séparation associée à une restriction du temps d'accés à l'alimentation : un outil pour l'étude des conséquences de l'anorexie mentale." Thesis, Littoral, 2014. http://www.theses.fr/2014DUNK0428/document.
Anorexia nervosa (AN) is an eating disorder mainly developed in adolescent girls and young women. It is characterized by an obsessive search for thinness, a profound undernutrition and a distorted self-image.It is associated with multiple endocrine and metabolic disturbances, decreased bone mass and microarchitectural alteration. Some of the developed adaptations are supposed to be involved in the blockade of the pathologic state. Unfortunately, these adaptations are poorly known and most of them cannot be studied on patients. So it is necessary to develop an animal model which mimics the main consequences observed in human pathology and allows studying the recovery process. For this purpose we adapted a murine model of time restricted feeding associated with chronic stress induced by separation-based anorexia (SBA). C57B1/6 female mice are submitted to a long term SBA protocol (10 weeks) and then a long term phase of recovery (10 weeks). At the beginning of the protocol mice are 8 weeks old, so their fast growth is finishing. SBA protocol induced a rapid and significant loss of body weight. Body composition analysis by DEXA showed a 40% decrease of the fat mass, a progressive loss of lean mass and a blockade of bone mass acquisition. Mice deveoped a high glucose tolerance. The observation of vaginal smears revealed a disruption of the estrous cycle and ovarian histology showed an atrophy of the ovaries. These two alterations suggest a major alteration of reproductive functions. These animals showed a very low leptinemia, and the GH/IGF-1 axis was disrupted. The study of bone alteration by microtomography indicated an alteration of bone microarchitecture and of cortical bone mass, mimicking osteoporosis often described in AN patients. Body weight, lean and fat masses were normalized quickly during the REC protocol. Bone mineral content still low after 2 weeks of REC protocol was fully corrected after 10 weeks. The estrous cycle ovarian size and the GH/IGF-I were normalized. Surprisingly, hypoleptinemia persisted even after 10 weeks of REC and despite the normalization of the fat mass. This result has been confirmed by the low level of leptin gene expression in various adipose tissues. Finally, the SBA protocol is valuable model of AN because numerous physiological alterations described in AN are mimicked in this model. The recovery phase revealed the high capacity of mice to normalize the long term alterations. Persitent hypoleptinemia could contribute to the normalization of body composition. However, the balance between central and peripheral effects of the uncorrected hypoleptinemia remains to be determined. This persisting hypoleptinemia could be used for the revision of the therapeutic strategies aiming to correct AN-induced osteoporosis
Pereira, Beatriz Aparecida Soares. "Patogenicidade e imunogenicidade de isolados clínicos do complexo Paracoccidioides brasiliensis." Botucatu, 2019. http://hdl.handle.net/11449/181206.
Resumo: Introdução. A correlação entre gravidade da paracoccidioidomicose e patogenicidade e imunogenicidade dos fungos causadores tem sido pouco investigada e foi o objetivo deste estudo. Metodologia. As cincos cepas Pb192, Pb234, Pb326, Pb417 e Pb531 foram identificadas pelo seqüenciamento da região Exon 2 da gp43. A patogenicidade foi determinada pelo cálculo da dose letal 50% (DL50%) e pela contagem do número de unidades formadoras de colônias, realizada na sexta semana pós-infecção de camundongos BALB/c. A imunogenicidade foi determinada pela avaliação da resposta imune humoral específica, utilizando-se a reação de imunodifusão dupla em gel de ágar e da imunidade celular, determinada pela concentração das citocinas interleucina -2, interleucina-10, interferon-γ, fator de necrose tumoral – α e do fator de crescimento do endotélio vascular, em tecido pulmonar. Quatro amostras clínicas foram recém-isoladas de pacientes com paracoccidioidomicose, provenientes da Região de Botucatu - os isolados Pb234 e Pb417, de pacientes com a forma crônica moderada; o Pb326 de um caso com a forma aguda grave; e o Pb531, de um caso com a forma crônica grave. As demais cepas Pb192, Pb01 e 8334 foram cedidas pelo laboratório de Moléstias infecciosas. Resultados. As cepas Pb417 e Pb326 agruparam-se às cepas identificadas como P. brasiliensis S1a, a Pb531 às P. brasiliensis S1b e as cepas Pb234 e Pb192 às cepas depositadas como P. restrepiensis (PS3). Os resultados demonstraram correlação direta entre ... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: Introduction. The investigation of paracoccidioidomycosis and pathogenicity and immunogenicity of the provoking fungi has been little investigated and was the objective of this study. Methodology. As strains, Pb192, Pb234, Pb326, Pb417 and Pb531 were included by sequencing the Exon 2 region of gp43. The pathogenicity was determined by calculating the 50% lethal dose (LD50%) and by counting the number of colony forming units performed in the sixth week post-infection of BALB / c mice. Immunogenicity was determined by the humoral immune response, using the agar gel immunodiffusion reaction and the cellular immunity, determined the concentration of cytokines interleukin-2, interleukin-10, interferonγ, tumor necrosis factor - and factor of vascular endothelial growth in lung tissue. Surgical has been associated with patients with paracoccidioidomycosis, derived from the Botucatu - the Pb234 and Pb417; the Pb326 of a case with a severe severe form; and Pb531, of a case with severe chronic form. The other strains Pb192, Pb01 and 8334 were transferred by the laboratory of Infectious Diseases. Results. Pb417 and Pb326 strains were grouped into the associated strains P. brasiliensis S1a, Pb531 to P. brasiliensis S1b and strains Pb234 and Pb192 to strains deposited as P. restrepiensis (PS3). The results demonstrate the pathogenicity of disease error and severity. The small LD 50 values were measured in the following cases: severe disease, Pb531 and Pb326. Pb531 strain was considered to... (Complete abstract click electronic access below)
Mestre
Silva, Muriel Vilela Teodoro. "Avaliação do efeito da expressão do gene da interleucina 32 (IL-32) humana em modelo murino de infecção por Leishmania (Leishmania) amazonensis." Universidade Federal de Goiás, 2016. http://repositorio.bc.ufg.br/tede/handle/tede/6333.
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Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEG
IL-32 is a proinflammatory cytokine which has different isoforms. IL-32γ isoform is the most powerful and was detected in lesions of patients with cutaneous leishmaniasis. Murine cells respond to IL-32, however mice lack the gene for this cytokine. To understand the role of IL- 32 in Leishmania (L.) amazonensis, we used transgenic mice for human IL-32γ (IL-32γTg). C57BL/6 mice (WT) and C57BL/6 IL-32γTg were infected with L. amazonensis promastigotes in the ear. The lesion development was followed weekly with a digital caliper (measured in mm of injury). After 3, 6 and 9 weeks, the animals were euthanized for tissue parasitism analysis by the limiting dilution technique, in infected ears, draining lymph node and spleen of mice. The draining lymph node cells were incubated (48 h) in the presence or absence of L. amazonensis antigen (Ag) for analysis of cytokines by ELISA. IL-32γTg mice present IL-32 production in spleen, liver, lymph node and ear. IL-32γTg mice have a lower injury than the WT mice during the third week of infection. From the 5th to the 9th week of infection, the two groups had similar lesion development profiles. Interestingly, in the 3rd week of infection, the parasitic load in the lesion of IL-32γTg mice was 100 times greater than that of WT mice. After three weeks, IL-32γTg mice maintained the same parasitic load up to nine weeks. In WT mice, however, the number of parasites increased exponentially during weeks evaluated. The parasite load in the spleen and lymph node was lower in IL-32γTg mice when compared with WT mice. There was no difference in histological sections of the lesions in WT and IL-32γTg mice infected with L. amazonensis. We did not observe differences between WT and IL-32γTg groups on the product -10) by lymph node cells stimulated with Ag, in the 3rd, 6th and 9th week of infection. Our data suggest that IL-32γ favors infection by L. amazonensis in the early stages, allowing the growth of the parasites. However, this cytokine seems to limit the growth and spread of parasites in the later stages of infection. In vitro analyzes show the similar percentage of infected cells and the number of parasites per infected cell in WT macrophages and IL-32γTg after 3 and 48h of infection with L. amazonensis. However, the production of NO by macrophages seems to be lower in IL- 32γTg mouse cells during infection with L. amazonensis. Understanding the mechanisms by which IL-32γ modulates Leishmania amazonensis infection in mice is essential to define the components that control cutaneous leishmaniasis caused by this specie in humans.
A IL-32 é uma citocina pró-inflamatória que apresenta diferentes isoformas. A isoforma IL- 32γ é a mais potente e foi detectada em lesões de pacientes com leishmaniose tegumentar americana. Células murinas respondem à IL-32, no entanto, camundongos não têm o gene para essa citocina. Para entender o papel da IL-32 na infecção por Leishmania (Leishmania) amazonensis, foram utilizados camundongos transgênicos para a IL-32γ humana (IL-32γTg). Camundongos C57BL/6 (WT) e C57BL/6 IL-32γTg foram infectados com formas promastigotas de L. amazonensis na orelha. O desenvolvimento da lesão foi acompanhado semanalmente com paquímetro digital (medida em mm de lesão). Após 3, 6 e 9 semanas, os animais foram eutanasiados para análise de parasitismo tecidual, pela técnica de diluição limitante, nas orelhas infectadas, no linfonodo drenante e no baço dos camundongos. As células do linfonodo drenante foram incubadas (48 h), na presença ou ausência de antígeno de L. amazonensis (Ag), para análise de citocinas pela técnica de ELISA. Camundongos IL- 32γTg apresentam produção de IL-32 no baço, fígado, linfonodo e orelha. Camundongos IL- 32γTg apresentam uma lesão menor do que a lesão dos camundongos WT, na terceira semana de infecção. Da 5ª até a 9a semana de infecção, os dois grupos apresentaram perfis semelhantes de desenvolvimento da lesão. Curiosamente, na 3ª semana de infecção, a carga parasitária na lesão do camundongo IL-32γTg era 100 vezes maior do que a dos camundongos WT. Após três semanas, os camundongos IL-32γTg mantiveram a mesma carga parasitária até nove semanas. Em camundongos WT, no entanto, o número de parasitos aumentou exponencialmente durante as semanas avaliadas. A carga parasitária do linfonodo e no baço foi menor nos camundongos IL-32γTg, quando comparado com camundongos WT. Não foi observada diferença nos perfis histológicos das lesões nos camundongos WT e IL-32γTg infectados por L. amazonensis. Não foi observada nenhuma diferença entre os grupos WT e IL-32γTg em relação à produção de citocinas (IFNγ, TNFα e IL-10), pelas células dos linfonodos estimuladas com Ag, na 3a, 6a e 9a semana de infecção. Os nossos dados sugerem que a IL-32γ favorece a infecção por L. amazonensis nas fases iniciais, permitindo o crescimento do parasito; no entanto, essa citocina parece limitar o crescimento e a disseminação dos parasitos nas fases mais tardias da infecção. As análises in vitro mostraram porcentagem de células infectadas e número de parasitas por célula infectada semelhantes nos macrófagos dos WT e IL-32γTg com 3 e 48h de infecção por L. amazonensis. Entretanto, a produção de NO por macrófagos parece ser menor nas células de camundongos IL-32γTg durante a infecção por L. amazonensis. Compreender os mecanismos pelos quais a IL-32γ modula a infecção por L. amazonensis nos camundongos é fundamental para a definição dos componentes que controlam a leishmaniose tegumentar causada por esta espécie em seres humanos.
Zapana, Priscila Rosse Mamani. "Desenvolvimento de um modelo murino para estudo da resposta imune conferida pela proteína do Nucleocapsídeo do vírus Oropouche." Universidade de São Paulo, 2017. http://www.teses.usp.br/teses/disponiveis/17/17147/tde-25042018-153023/.
Oropouche (OROV) is an arbovirus that occurs in the South American, Amazon region, producing outbreaks of acute febrile illness occasionally associated to meningoencephalitis. Approximately 500,000 cases of Oropouche have been reported in Brazil in the last 60 years. However, there is no available vaccine for OROV. We show here the development of an animal model of OROV suitable for studies on pathogenesis and vaccine testing. A vaccine prototype based on recombinant OROV nucleocapsid protein (NrOROV), an important viral antigen, was evaluated in the animal model. Initialy, we observed that all 12-week-old Balb/c mice inoculated intracerebrally with 8x105 PFU died after the third day of infection. Surprisingly, OROV genome was detectable in the liver as early as 12 hours post infection (pi) and in the spleen at 24 hours pi at 3 log10 PFU/g. Besides, high viral titers were found in brain and spinal cord. To test the NrOROV as a vaccine candidate, animals divided in 5 groups were immunized subcutaneously 3 times, two weeks apart with either OROV, OROV and Freud complete Adjuvant (FCA), NrOROV, NrOROV and FCA, NrOROV and Poly I:C and Montanide ISA 720. The experiment also included a group of naïve animals. After the third immunization, the animals were challenged with 10LD50 by intracerebral route and followed for 20 days. The animals immunized with NrOROV and adjuvants developed specific antibodies that were not able to neutralize the virus or confer protective immunity against OROV. Nevertheless, mice immunized with OROV showed high levels of neutralizing and protective antibodies. Despite the discouraging results with NrOROV as a vaccine, the mouse model is suitable to study pathogenesis, and to test other vaccines for OROV.
DE, PONTI GIADA. "Exploring early therapeutic approaches in a Mucopolysaccharidosis type I (MPS I) mouse model." Doctoral thesis, Università degli Studi di Milano-Bicocca, 2022. http://hdl.handle.net/10281/382061.
The present PhD project has taken into account critical issues around Mucopolysaccharidosis type I (MPSI) and limitations of current therapies to further improve them, by generally focusing on neonatal therapeutic approaches, both in terms of combined HSCT and ERT and of gene therapy, and on trying to reduce the overall toxicities associated with pre-conditioning settings. Overall, the most important open issues regarding this rare life-threatening disorder are the need for a precocious and rapid intervention, the lack of complete disease correction after current therapeutic approaches and side effects due to pre-conditioning regiment. My PhD project partially focused on testing a combined approach of the current standard-of-cares for treating MPSI. HSCT and ERT combination efficacy was tested in a mouse model of MPSI as neonatal intervention, for evaluating additional benefits of continuous enzyme therapy after transplant of donor’s cells. We compared three treatment options starting from MPSI pups’ birth, considering IDUA deficient activity, GAGs storage and vacuoles in visceral organs, the immune response against the recombinant IDUA and skeletal and CNS ameliorations. Therefore, performing a combined approach of HSCT and ERT in the neonatal period could help improving some hard-to-treat MPSI manifestations. The second part of this PhD project was carried out in collaboration with TIGET-SR and Prof. Alessandro Aiuti. It was focused on testing a neonatal gene therapy approach in a mouse model of MPSI, considering the importance of an early phenotype correction. In particular, we evaluated if this early treatment could be applied in MPSI neonates and could be a successful strategy for overcoming the main clinical issues that still remain after current canonical HSCT treatment. We monitored peripheral blood of treated mice for 8 months in terms of enzymatic activity and VCN, and we evaluated the effect of GT performed in MPSI pups at endpoint, considering IDUA deficient activity, vector copies/genome, GAGs storage and vacuoles in visceral organs, the immune response against the recombinant IDUA and skeletal and CNS ameliorations. The last part of this PhD project was centered on trying to reduce the high morbidity and mortality due to the severe conditioning regimen in the context of neonatal MPSI therapies, as a side project. The main objective was to translate the application of hematopoietic cell–specific antibody-drug conjugates (ADCs) as conditioning for early MPSI treatment, in which a precocious intervention is crucial. Since none of the tested setting was able to induce enough engraftment of donor’s cells to be relevant for MPSI early treatment, we tried to understand what could interfere, but we demonstrated the need for further studies prior to ADCs application in humanised models and in MPSI NSG pups. Preliminary results on increased cytokines levels after CD117-SAP in adult NSG compared to other conditioning settings were performed to evaluate impairment of inflammation and possible ADC application with anti-inflammatory drugs prior to early therapy in MPSI pups.
Palma, Luana Carneiro. "Doença esteatóica não alcoólica do fígado: comparação das alterações histológicas hepáticas entre modelo murino e pacientes obesos." Centro de Pesquisas Gonçalo Moniz, 2013. https://www.arca.fiocruz.br/handle/icict/7149.
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Universidade Federal da Bahia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
A Doença Esteatótica Não Alcoólica do Fígado (do inglês Nonalcoholic Fatty Liver Disease – NAFLD) é uma doença crônica hepática de caráter espectral, que vai desde a esteatose simples até a esteato-hepatite não alcoólica. A progressão para cirrose e carcinoma hepatocelular têm sido descrita. A NAFLD apresenta aspectos histológicos semelhantes à doença hepática relacionada ao álcool (esteatose, inflamação lobular, corpúsculos de Mallory e fibrose), mas acomete indivíduos com história negativa de consumo excessivo de álcool. A NAFLD é uma das principais doenças crônicas hepáticas mundiais, e os indivíduos obesos representam a maioria dos casos da doença. Os mecanismos envolvidos na progressão da esteatose para esteato-hepatite não são bem compreendidos. Neste aspecto, modelos murinos da NAFLD têm sido frequentemente utilizados para elucidação destes mecanismos. A maioria dos modelos disponíveis é resultante de modificações genéticas e/ou nutricionais e, em geral, não simulam as alterações metabólicas e histológicas comumente vistas em pacientes com NAFLD. Em nosso grupo, foi proposto um novo modelo de NAFLD. Camundongos C57BL/6 alimentados com dieta rica em gordura (High Fat - HF) demonstraram alterações metabólicas e histológicas sugestivas de NAFLD. O objetivo do presente trabalho foi comparar alterações histológicas hepáticas presentes nestes camundongos com as alterações observadas em pacientes obesos. Amostras de fígados de pacientes obesos e de camundongos alimentados com a dieta HF foram utilizadas. Os tecidos hepáticos foram corados em Hematoxilina & Eosina e Picrossírius Red para avaliação das alterações hepáticas (esteatose, balonização, inflamação, corpúsculos de Mallory-Denk e fibrose). Além disso, foi realizada imunoistoquímica para avaliação da presença de células estrelares ativadas e de células progenitoras hepáticas, células envolvidas na fibrose e no desenvolvimento de carcinoma hepatocelular, respectivamente. Os resultados demonstraram que os fígados de todos os pacientes obesos exibiram esteatose macrovacuolar, balonização hepatocelular, inflamação lobular e fibrose perissinusoidal, o que caracterizou estes pacientes como portadores da NAFLD. As mesmas alterações foram observadas em fígados de camundongos alimentados com a dieta HF. As células estrelares ativadas foram observadas em todos os pacientes obesos, assim como em camundongos de dieta HF. As células progenitoras hepáticas foram observadas na maioria dos pacientes obesos. O fígado de todos os camundongos alimentados com dieta HF exibiram células progenitoras hepáticas. A partir dos dados obtidos, pode-se concluir que fígados de camundongos alimentados com dieta HF exibem alterações histológicas hepáticas similares às observadas em pacientes obesos. Isto abre perspectivas para a utilização do modelo proposto em estudos que busquem elucidar os mecanismos envolvidos na patogênese da NAFLD.
Nonalcoholic Fatty Liver Disease (NAFLD) is a chronic liver disease ranging from simple steatosis to nonalcoholic steatohepatitis. The progression to cirrhosis and hepatocellular carcinoma has been reported. The NAFLD shows histological features similar to alcohol-related liver disease (steatosis, lobular inflammation, fibrosis and Mallory-Denk bodies), but affects individuals with no history of excessive alcohol consumption. The NAFLD is a major chronic hepatic disease in the world, and obese individuals represent the majority of cases of the disease. The mechanisms involved in the progression of steatosis to steatohepatitis are not well understood. In this regard, murine models of NAFLD have been frequently used for elucidation of these mechanisms. Most available models are the result of genetic or nutritional modifications, and generally do not mimic metabolic and histologic changes commonly seen in patients with NAFLD. In our group, we have proposed a new model of NAFLD. Mice fed high fat diet (HF diet) demonstrated metabolic and histological features suggestive of NAFLD. The aim of this study was to compare liver histological alterations present in these mice with the changes observed in obese patients. Samples of livers of obese patients and mice fed HF diet were used. For assessment of liver alterations, such as steatosis, ballooning, inflammation, Mallory- Denk bodies and fibrosis, tissues were stained with hematoxylin & eosin and picrossirius red. In addition, the presence of activated stellate and progenitor liver cells was estimated using immunohistochemistry. The results show that the livers of all obese patients exhibited macrovesicular steatosis, hepatocellular ballooning, perisinusoidal fibrosis, and lobular inflammation, which characterized these patients with NAFLD. Similar changes were observed in livers of mice that fed the HF diet. Activated stellate cells were observed in all obese patients as well as in mice HF. Hepatic progenitor cells were observed in most obese patients. The liver of all animals fed the HF diet exhibited liver progenitor cells. From the data obtained, it can be concluded that livers of mice fed with HF diet exhibit liver abnormalities similar to those observed in obese patients. This opens perspectives for the use of the proposed model in studies that seek to elucidate the mechanisms involved in the pathogenesis of NAFLD.
Fercoq, Frédéric. "Interactions filaire/poumon dans le modèle murin de filariose Litosomoides sigmodontis." Thesis, Paris, Muséum national d'histoire naturelle, 2017. http://www.theses.fr/2017MNHN0018/document.
Filariae are parasitic nematodes transmited to vertebrates by haematophagous arthropods. The filarial species that settle in the coelomic cavities, the lymphatic vessels or the connective tissues have their infectious stages (or L3) which migrate via the lymphatic system after their inoculation into the skin. Using the murine model with the filaria Litomosoides sigmodontis, whose adults reside in the pleural cavity, two phases of interaction between filariae and the lung of BALB/c mice are described 1) during the L3 migration from the skin to the pleural cavity ; 2) during the patent phase of infection, when adults realease microfilariae in the pleural cavity. During the 1st phase L3 join the pulmonary blood system and then cross through the lungs to enter the pleural cavity. This passage induces a transient acute pathology: first haemorrhages following the rupture of the pulmonary capillaries, together with an increase in the number of pulmonary neutrophils and the transient release of IL-1β and the alarmins IL-33 and S100A9 in the pleural cavity. S100A9 appears to facilitate the survival of the filariae either by an anti-inflammatory effect or by facilitating the migration of L3. Neutrophils can release NETs in response to L3. Within days following the infection, a regulatory response takes place in the lungs, with recruitment of macrophages and eosinophils, production of IL-4, CCL2 and IL-9, and downregulation of inflammatory molecules. The formation of granulomas is also observed in pulmonary tissue. The passage of L3 also induces an inflammation of pulmonary blood vessels, in C57BL/6 mice only. During the patent phase of the infection, 40% of the mice do not develop blood microfilaraemia. Comparison of responses of microfilaremic and amicrofilaremic mice shows an exacerbation of pleural inflammation induced by microfilariae. In addition, microfilaremic mice develop microfilaria-dependent pulmonary pathology consisting on fibrosis of the visceral pleura, perivascular accumulation of macrophages and bronchoalveolar inflammation (mucus production and eosinophilia). The control of the filariae (adults and microfilariae), but also the establishment of the pathology are dependent on IL-5 and IL-4R
Babaie, Yasmin. "A murine model for haemangioblast transplantation." Thesis, University of Edinburgh, 2004. http://hdl.handle.net/1842/23691.
Watkins, Alan D. "A murine model of pulmonary inflammation." Thesis, University of Southampton, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.395963.
Bahdoudi, Seyma. "Etude des mécanismes cellulaires et moléculaires impliqués dans les effets neuroprotecteurs du gliopeptide OctaDecaNeuropeptide (ODN) dans un model murin de la Maladie de Parkinson." Thesis, Normandie, 2017. http://www.theses.fr/2017NORMR138/document.
Parkinson's disease (PD) is a neurodegenerative disorder characterized by a progressive loss of loss of dopaminergic (DA) neurons within the substantia nigra pars compacta (SNpc). Different mechanisms are associated with the neuropathogenesis of PD including dysfunction of the mitochondrial respiratory chain, oxidative stress, apoptosis and neuroinflammatory processes. Octadecaneuropeptide (ODN) is a diazepam-binding inhibitor (DBI)-derived peptide, expressed by astrocytes, which protects neurons against oxidative cell damages and apoptosis in an in vitro model of PD. Nevertheless, its protective action in vivo has never been investigated. Therefore, the aim of the project of this thesis was to investigate whether intracerebroventricular (i.c.v) injection of ODN could prevent DA neuron degeneration in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model of PD, and to explore the vulnerability of ODN precursor knockout (DBI KO) mice to MPTP-induced neurotoxicity. The results show that a single i.c.v injection of 10 ng/μl ODN, 1 h after the last systemic administration of MPTP, prevents the reduction of the number of tyrosine hydroxylase (TH)-positive cell bodies and fibers in the SNpc and striatum, respectively. Immunofluorescence imaging, Western blot analysis and Q-PCR studies revealed that ODN totally abolished MPTP-induced decrease of TH positive cells, mRNA expression and protein levels. This neuroprotective effect of ODN is accompanied by a reduction in the number of reactive astrocytes, an inhibition of the expression of pro-inflammatory genes such as interleukins (IL) IL-1β and IL-6, and a decrease of tumor necrosis factor -α. In addition, ODN blocks the inhibition of the anti-apoptotic Bcl-2 gene and the stimulation of Bax and caspase-3 expression induced by MPTP in the SNpc and striatum. ODN also reduces the accumulation of reactive oxygen species (ROS) and lipid oxidation products in DA neurons. Furthermore, DBI-/- mice exhibited more vulnerability to MPTP than wild-type animals (DBI+/+). Thus, ODN KO mice are more sensitive to MPTP-induced inflammatory and oxidative brain damages, suggesting that the endogenous OD may also be neuroprotective. These results indicate that, based on its anti-oxidative, anti-inflammatory and anti-apoptotic effect, the gliopeptide ODN could lead to the development of effective therapeutic agents for the treatment of cerebral injuries involving oxidative neurodegeneration
Nicolas, Sarah. "Mise en évidence du potentiel thérapeutique de l’adiponectine et de son rôle dans les effets antidépresseurs de l’environnement enrichi." Thesis, Université Côte d'Azur (ComUE), 2018. http://www.theses.fr/2018AZUR4019/document.
Major depression is a complex disorder characterized by behavioral and cognitive impairments triggered by various factors including genetic predispositions, stress and environment. The pathophysiology of depression is poorly understood. Numerous evidence suggests that neuroinflammation is associated with depression. Alternative therapeutic strategies are needed and "positive" life experiences could be an efficient way to help the remission of the disorder. To study the potential antidepressant effects of such “positive” living conditions, we used the enriched environment (EE) paradigm on mice. The aim of our work was to fully characterize the antidepressant and anti-inflammatory effects of EE in a well-characterized murine model of depression-like behavior induced by long-term administration of corticosterone. We showed that EE efficiently reverses the anxiety/depression‐like state of mice and reduces neuroinflammation. Moreover, we identified the adipokine Adiponectin as a key player in the beneficial effects of EE. We reported that increased levels of Adiponectin in the brain led to microglia phenotype and activation state regulation, thus reducing global brain inflammation in mice. Indeed, the anti-inflammatory and antidepressants effects of EE are abolished in Adiponectin deficient mice. We demonstrated that anti-inflammatory actions of Adiponectin on microglia is mediated through the Adiponectin Receptor 1. Those results highlight the key role of the adiponergic system in the treatment of psychiatric disorders. Therefore, we tested the effect of AdipoRon, a potent Adiponectin receptors 1 and 2 agonist on corticosterone-treated mice. AdipoRon successfully reversed the corticosterone-induced depression-like state in mice. AdipoRon exerted its pleiotropic actions on various systems including hippocampal neurogenesis, serotonergic neurotransmission and neuroinflammation, which can explain its antidepressant properties. Together, our findings bring insight into the beneficial effects of "positive" life experiences in depression and neuroinflammation, highlight the pivotal role of Adiponectin pathway and emphasizes that AdipoRon or other Adiponectin receptor agonist may constitute a promising novel antidepressant
Zychlinska, Magdalena. "Murine Model System for EBV-related Diseases." Diss., lmu, 2007. http://nbn-resolving.de/urn:nbn:de:bvb:19-73499.
Barnard, Amanda Louise. "A murine model for immunotherapy of melanoma." Thesis, King's College London (University of London), 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298460.
Levison, Scott. "Characterization of a murine model of colitis." Thesis, University of Manchester, 2012. https://www.research.manchester.ac.uk/portal/en/theses/characterization-of-a-murine-model-of-colitis(4b966667-a763-4bcc-8896-7a157cf3d3dd).html.
Archelus, Anderson. "Rôle de l’environnement microbien dans la régulation de l’immunoglobuline A mucosale et dans le développement de la maladie de Berger." Thesis, Limoges, 2018. http://www.theses.fr/2018LIMO0007/document.
IgA nephropathy is the most frequent glomerulonephritis worldwide. It features mesangial immunoglobulin (Ig)A deposits and proteinuria, hematuria and glomerular histological lesions. In 25% patients, it evolves, within 20 years, towards the end stage renal disease. Microbial environment, through the interaction with mucosa, is believed to play a crucial role in the development of the disease. The objectives of my work were to evaluate the effect of the microbial compound lipopolysaccharide (LPS) on the production of the nephritogenic IgA in the mucosa of mice and on the development of IgA nephropathy in the murine model 1KI that spontaneously develops mesangial IgA deposits without the other signs of the disease. Our results and those previously published suggest that the chronic stimulation of mucosa by microbiota leads to the increased production of nephritogenic IgA in the mucosa. These IgA, thanks to a deficient mucosal receptor pIgR in patients with IgA nephropathy, may be abnormally routed in the blood. We also showed that LPS provokes hematuria in the 1KI mice, when they express a specific IgA receptor, CD89, on the surface of polymorphonuclear neutrophils. Altogether our findings highlight the impact of microbial environment on pIgR and on polymorphonuclear neutrophils and thus, potentially, on IgA nephropathy
Helou, Doumet. "Rôle du facteur de transcription Nrf2 dans la régulation des fonctions du neutrophile in vitro et dans l’allergie cutanée." Thesis, Université Paris-Saclay (ComUE), 2018. http://www.theses.fr/2018SACLS305/document.
Neutrophils form the first line of defense against infectious agents. However, their uncontrolled activation may exacerbate certain inflammatory conditions such as cutaneous allergies. Our team has previously shown that Nrf2 transcription factor known for its antioxidant role, regulates skin inflammation in contact hypersensitivity (CHS). Thus, our work was carried out to evaluate in vitro the involvement of Nrf2 pathway in neutrophil functions and to identify Nrf2 role in neutrophil recruitment and activation in CHS.In vitro, we showed that the protein Nrf2 was highly expressed in bone marrow neutrophils. Nrf2 is functional in stimulated neutrophils: it activates the transcription of cytoprotective genes and downregulates that of inflammatory genes. Thus, pretreatment of neutrophils with an Nrf2 activator such as sulforaphane reduces the production of reactive oxygen species (ROS) in response to stimulation. In parallel, Nrf2 does not affect two key functions of neutrophil, phagocytosis and netosis.Finally, Nrf2 is essential for optimal migration of neutrophils toward chemokines. In CHS induced by the dinitrochlorobenzene (DNCB), Nrf2 indirectly regulates the recruitment of neutrophils, through regulation of skin oxidant stress and inflammatory pathways that are involved in chemokines production, including CCL2, CCL4 and CCL11. In addition, Nrf2 induces the up-regulation of scavenger CD36 in macrophages and thus increases their ability to eliminate apoptotic neutrophils leading to the resolution of inflammation.In conclusion, Nrf2 activation in neutrophils participates in the control of ROS production and migration. In addition, Nrf2 emerges as an important effector in the control of neutrophil recruitment and clearance during the skin inflammatory response to allergenic molecules. The demonstration of Nrf2 protective mechanisms leads us to suggest this protein as a new therapeutic target in the control of chronic skin inflammations
Nicolas, Sarah. "Mise en évidence du potentiel thérapeutique de l’adiponectine et de son rôle dans les effets antidépresseurs de l’environnement enrichi." Electronic Thesis or Diss., Université Côte d'Azur (ComUE), 2018. http://theses.univ-cotedazur.fr/2018AZUR4019.
Major depression is a complex disorder characterized by behavioral and cognitive impairments triggered by various factors including genetic predispositions, stress and environment. The pathophysiology of depression is poorly understood. Numerous evidence suggests that neuroinflammation is associated with depression. Alternative therapeutic strategies are needed and "positive" life experiences could be an efficient way to help the remission of the disorder. To study the potential antidepressant effects of such “positive” living conditions, we used the enriched environment (EE) paradigm on mice. The aim of our work was to fully characterize the antidepressant and anti-inflammatory effects of EE in a well-characterized murine model of depression-like behavior induced by long-term administration of corticosterone. We showed that EE efficiently reverses the anxiety/depression‐like state of mice and reduces neuroinflammation. Moreover, we identified the adipokine Adiponectin as a key player in the beneficial effects of EE. We reported that increased levels of Adiponectin in the brain led to microglia phenotype and activation state regulation, thus reducing global brain inflammation in mice. Indeed, the anti-inflammatory and antidepressants effects of EE are abolished in Adiponectin deficient mice. We demonstrated that anti-inflammatory actions of Adiponectin on microglia is mediated through the Adiponectin Receptor 1. Those results highlight the key role of the adiponergic system in the treatment of psychiatric disorders. Therefore, we tested the effect of AdipoRon, a potent Adiponectin receptors 1 and 2 agonist on corticosterone-treated mice. AdipoRon successfully reversed the corticosterone-induced depression-like state in mice. AdipoRon exerted its pleiotropic actions on various systems including hippocampal neurogenesis, serotonergic neurotransmission and neuroinflammation, which can explain its antidepressant properties. Together, our findings bring insight into the beneficial effects of "positive" life experiences in depression and neuroinflammation, highlight the pivotal role of Adiponectin pathway and emphasizes that AdipoRon or other Adiponectin receptor agonist may constitute a promising novel antidepressant
Carollo, Maria. "Investigation of chemokine receptor expression in an experimental model of chronic granulomatous inflammation." Thesis, Queen Mary, University of London, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.249652.
Koval, Lidia. "Investigating the effect of internal fixation stiffness on metaphyseal fracture healing in a mouse model." Thesis, Queensland University of Technology, 2014. https://eprints.qut.edu.au/75644/1/Lidia_Koval_Thesis.pdf.
Bienvenu, Anne-Lise. "Neuroprotection et neuropaludisme des souris et des hommes." Thesis, Lyon 1, 2009. http://www.theses.fr/2009LYO10062.
There are at least 300 million cases of malaria each year, resulting in more than a million deaths. Cerebral malaria is the most severe complication of malaria especially in young African children. Besides drug resistance, another challenge in the fight against malaria is the protective treatment of the host combined to the conventional antimalarial treatment. This opportunity came with the consideration of cerebral malaria residual case fatality rate of about 20%, despite a timely adequate antimalarial treatment. This work describes the available experimental model of cerebral malaria, the place of neuroprotective therapies in this disease and the efficacy of two neuroprotective drugs, recombinant erythropoietin and statins, during experimental cerebral malaria. Last, a proof-of-concept study documented the relevance of neuroprotection during human cerebral malaria in endemic area
Healy, D. M. "Comparative pathology of Lyssaviruses in a murine model." Thesis, Queen's University Belfast, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.546358.
Beer, Abigail J. "Development of an Inducible c-MYC Murine Model." The Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1602754882137456.
Gibbons, Peter Luke. "Development of a Pharmacodynamic Model of Murine Malaria." Thesis, Curtin University, 2015. http://hdl.handle.net/20.500.11937/1031.
Rebouças, Mônica Cardozo. "Resposta à quimioterapia com benzonidazol em camundongos com tríplice infecção por clones da cepa 21SF (São Felipe / Bahia) do trypanosoma cruzi." Centro de Pesquisas Gonçalo Moniz, 2012. https://www.arca.fiocruz.br/handle/icict/7162.
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Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
Populações que residem em áreas endêmicas da doença de Chagas são submetidas a múltiplas infecções pelo Trypanosoma cruzi e podem estar infectadas com cepas ou clones com diferentes graus de virulência e susceptibilidade à quimioterapia. Este é um fator importante para o desenvolvimento e morbidade da doença. As cepas de Trypanosoma cruzi representam complexas populações multiclonais, homogêneas ou heterogêneas, com predominância de um clone principal. São biologicamente classificados em Biodemas (Tipos I, II e III) que apresentam diferentes graus de resistência à quimioterapia. Cepas do Tipo I são muito suscetíveis ao tratamento; cepas do Tipo II possuem uma média susceptibilidade (cepa 21SF); cepas de Tipo III são muito resistentes (cepa Colombiana). No presente estudo, é avaliado o resultado do tratamento de camundongos triplamente infectados com clones da cepa 21SF, em comparação com os infectados com a cepa parental. 50 camundongos foram infectados com a cepa 21SF (infecção única). O inóculo foi de 5 x 104 formas tripomastigotas sanguícolas. 80 camundongos foram infectados, sucessivamente, com 3 clones da cepa 21SF (C6, C7 e C8), inóculo: 1x104 formas tripomastigotas (infecção tripla). A infecção simples com cada clone também foi feita. Os camundongos de ambos os grupos foram divididos em dois subgrupos: tratados com Benzonidazol – BZ (100 mg/kg/dia - 60 doses) e controles não tratados. Após 60 dias do final do tratamento, os camundongos sobreviventes foram eutanasiados, por exsanguinação, pós-anestesia; o sangue foi coletado para o exame sorológico de imunofluorescência indireta; testes de cura foram realizados (parasitemia, xenodiagnósticos, hemocultura) e seções do coração e músculo esquelético foram coletadas, fixadas e processadas para o estudo histopatológico em cortes corados com Hematoxilina & Eosina. A PCR foi também usada como uma técnica diagnóstica complementar. Os testes parasitológicos mostraram uma positividade variando de 54,4% nos camundongos infectados com a cepa parental e tratados; 33,4 a 66,7% nos animais com infecção única pelos diversos clones e tratados e 26,7% nos camundongos com infecção tríplice, tratados. Os títulos sorológicos (TIFI) variaram de 1:20 a 1:280 nos infectados com cepa parental tratados com BZ e de 1:640 a 1:1280 para controles não tratados. Os títulos sorológicos na infecção única com cada clone variaram de 1:10 a 1:1280 em camundongos tratados e de 1:160 a 1:1280 nos controles não tratados. A PCR revelou positividade em todos os animais infectados, tratados. O resultado final foi obtido pela combinação dos testes parasitológicos com os títulos sorológicos revelando positividade de 6,6% nos camundongos infectados com a cepa parental e tratados; 0 a 18,2% nos animais com infecção única pelos diversos clones e tratados e 12% nos camundongos com infecção tríplice, tratados. Estudo histopatológico: Os camundongos infectados com a cepa parental apresentaram lesões que variavam de leves a moderadas, na maioria dos casos, predominante no miocárdio (animais tratados e controles não tratados) camundongos submetidos à infecção única por cada clone apresentaram lesões semelhantes aos demonstrados pela infecção com cepa parental. Os camundongos com infecção tripla apresentaram uma exacerbação de lesões, evoluindo para a miocardite crônica. Nestes casos, havia intensas lesões no músculo esquelético; animais tratados apresentaram uma nítida redução das lesões no miocárdio e no músculo esquelético. Os resultados da quimioterapia com Benzonidazol em animais triplamente infectados, considerando os testes parasitológicos e sorológicos, revelaram baixos índices de cura e agravamento das lesões tissulares nos camundongos submetidos a múltiplas infecções com clones obtidos da cepa 21SF.
People living in endemic areas of Chagas disease are submitted to multiple infections during their lives and could be infected with strains or clones with different virulence and susceptibility to chemotherapy. This is an important factor in the development and morbidity of the disease. Strains of Trypanosoma cruzi represent complex multiclonal populations, which can be homogeneous or heterogenous with predominance of a principal clone. The strains are biologically classified in different Biodemes (Types I, II and III) which disclose different degrees of resistance to chemotherapy. Type I strains are very susceptible to treatment; Type II strains disclose medium susceptibility (21SF strain); strains of Type III are very resistant (Colombian strain). The clones isolated from different strains can also present different degrees of resistance. In the present study the results of treatment of mice triple infected with clones of the 21SF strain is evaluated, in comparison with the infected with the parental strain. For the present study, 50 Swiss mice were infected with the 21SF strain (single infection).The inoculum was of 5 x 104 blood trypomastigotes. 80 mice were infected successively with 3 clones of the 21SF strain (C6, C7 and C8) inoculum: 1x104 trypomastigotes (triple infection). Single infection with each Clone was also done. The mice of both groups were divided into 2 sub-groups: treated with Benzonidazol –BZ (100mg/kg/day – 60 doses) and untreated controls. After 60 days of the end of treatment, surviving mice were killed by exsanguinations after anesthesia; the blood was collected for indirect immunofluorescence serological test; cure tests were performed (parasitemia, xenodiagnosis, haemoculture) and sections of the heart and skeletal muscle were collected, fixed and then processed for the histopathological study in sections stained with Hematoxilin end Eosin. PCR reaction was also performed in blood samples as a complementary diagnostic technique. Parasitological tests have shown positive results that varied from 54.4% for the group of mice infected with the parental strain and treated with BZ; 33.4 to 66.7% for the mice with single infection, with each clone, treated with BZ, and 26.7% with triple infection with the three clones, treated with BZ. Serology titers (IIFT) varied from 1:20 a 1:280 for the mice infected with parental strain treated with BZ and from 1:640 to 1:1280 for untreated controls. Serology titers in the single infection with each clone varied from 1:10 to 1:1280 in treated mice and from 1:160 to 1:1280 in the untreated controls. PCR reaction was positive in all the infected mice, treated. Parasitological tests combined with the sorological titers have shown a positivity of 6.6% for the mice infected with the parental strain and treated with BZ; 0 to 18.2% for the mice with single infection with each clone and treated an 12% for the mice with triple infection and treated with BZ. Histopathological examination revealed in mice infected with the parental strain, mild to moderate inflammatory lesions. Mice submitted to single infection with clones of the 21SF strain presented similar lesions to those determined by infection with parental strain. Mice with triple infection present an intensification of lesions, and evolution to a chronic myocarditis and intense lesions in skeletal muscle; Treated animals presented clear reduction of lesions in myocardium and in skeletal muscle. Results of chemotherapy with BZ in mice with triple infection with clones of the 21 SF T. cruzi strain, considering the results of parasitological and serological tests, revealed low cure rates and aggravation of tissue lesions in those animals submitted to triple infections with clones of the parental strain.
Moore, Brioni R. "Pharmacodynamic studies of antimalarial drugs in a murine malaria model." Thesis, Curtin University, 2011. http://hdl.handle.net/20.500.11937/699.
Delhon, Laure. "Rôle d’ADAMTSL2 et FBN1 dans l’ossification endochondrale : étude des modèles murins mimant la dysplasie géléophysique." Thesis, Sorbonne Paris Cité, 2017. http://www.theses.fr/2017USPCB081/document.
Geleophysic dysplasia (GD) is a rare disease, which belong to acromelic group. This pathology is characterized by short stature, brachydactyly, joint stiffness, thick skin, facial dimorphism, broncho-pulmonary insufficiency and cardiac disease which lead to an early death in the first years of life. Two mode of inheritance have been identified. The first one, autosomal recessive, is due to mutations in ADAMTSL2 gene. The second, autosomal dominant, is due to hot-spot mutations in exon 41-42 of FBN1 gene, which encode the Transforming Growth Factor (TGF) β-binding protein-like domain 5 (TB5) of the protein. FBN1 and ADAMTSL2 encode secreted proteins of the extracellular matrix (ECM). FBN1 encodes fibrilline-1, component of microfibrillar network, playing a role in the bioavailability of TGF- β. ADAMTSL2 protein belongs to ADAMTS family, but does not have enzymatic activity due to lack of catalytic domain. Its function remains unknown. However, ADAMTSL2 partners have been identified by our team: latent-transforming growth factor beta-binding protein 1 (LTBP1) and FBN1, which are directly implied in storage of TGF-β. Recently, another protein, FBN2, have been identified as an ADAMTSL2 partner (Hubmacher D et. al.). The aim of my study was to understand the physiopathological mechanism of Geleophysic dysplasia by analysing murine models. A first murine model for the GD recessive form, CreCMV; Adamtsl2f/f (KO), have been generated. Phenotypic analysis of these mice showed short stature and shorter long bones and extremities. In long bone growth plate of mutant mice, we observed disorganization of chondrocyte columns, associated with decrease of collagen 10 expression, marker of chondrocyte differentiation. Analysis of ECM in growth plate revealed strong structural disorganization. Decrease of FBN1 and LTBP1 and were observed with an overactivation of TGF-β pathway in growth plate of mutant mice. We observed disorganization of microfibrillar network in chondrocyte cultures of mutant mice. These results suggest that ADAMTSL2 protein is implied in structure of microfibrillar network, where is stored TGF-β, and demonstrate major role of ADAMTSL2 in chondrogenesis. In order to study dominant form of GD, mouse model FBN1TB5+/-, have been generated. The mice were obtained by knock-in system, with mutation in exon 42 of FBN1 gene. Our results showed short stature of heterozygous (HT) and homozygous (Ho) mice compared to wild)type mice, at stage P1 and P30. At stage P1, we observed larger chondrocytes and deregulation of chondrogenesis markers in growth plate of HT and Ho mice. Furthermore, we observed high mortality of Ho mice at 2-3 months. We concluded that mutations in TB5 domain of FBN1 were linked to short stature and thus FBN1 have major role in chondrogenesis
Sanlaville, Amélien. "Rôle de la réponse immunitaire adaptative anti-tumorale dans l’induction de la transition épithélio-mésenchymateuse." Thesis, Lyon, 2016. http://www.theses.fr/2016LYSE1276.
Current clinical challenge in many carcinomas is to reduce the risk of metastasis development and cancer recurrence. Epithelial-mesenchymal transition (EMT), a physiological process during embryogenesis, is a central mechanism in oncogenesis. EMT induction contributes to early transformation and dissemination through inhibition of cellular surveillance (apoptosis and senescence) and increased migrative and invasive behavior. Another necessary hallmark of cancer is the ability of tumor cells to evade immune surveillance, a powerful barrier against tumor progression. But cancer cells enjoy intricate relations with the immune system. Whereas inclination of inflammation and innate immune cells to favor tumor development and immune escape, via EMT induction and immunosuppressive microenvironment maintenance, has been well investigated, the role of adaptive immune response in EMT promotion is understudied. Based on the development of a plastic murine mammary tumor cell line model overexpressing Her2/Neu oncogene, this study demonstrate in vivo that tumor cells keep an epithelial phenotype in adaptive immunodeficient mice but undergo EMT under the pressure of T-cell mediated immune response, characterized by loss of epithelial EpCAM marker and acquisition of mesenchymal features and EMT transcriptomic signature. CD4 T cell depletion but not CD8 restores the epithelial phenotype of tumors, suggesting that CD4 T cells mediate an immune response that could lead ton EMT induction. In return, EMT confers the ability of tumor cells to shape immunity like intra-tumor neutrophil infiltration. This work shed a new light on interactions between tumor cells and immune system
Luzzi, Keith J. "Quantification of metastatic inefficiency in a murine liver model." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/mq21109.pdf.
Holland, T. W. C. "The pathogenesis of a murine model of rheumatoid arthritis." Thesis, University of Nottingham, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.330136.
Davami, Mohammad Hassan. "Immunology of crytopsoridiosis : studies with a murine infection model." Thesis, London School of Hygiene and Tropical Medicine (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267888.
Franklin, Sarah Louise. "The extracellular matrix in a model of murine scrapie." Thesis, University of Bristol, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.440256.
Hynes, Ann Marie. "Investigating nephronophthisis using a novel murine and cell model." Thesis, University of Newcastle upon Tyne, 2014. http://hdl.handle.net/10443/2370.
Rutter, Erica M., Tracy L. Stepien, Barrett J. Anderies, Jonathan D. Plasencia, Eric C. Woolf, Adrienne C. Scheck, Gregory H. Turner, et al. "Mathematical Analysis of Glioma Growth in a Murine Model." NATURE PUBLISHING GROUP, 2017. http://hdl.handle.net/10150/624455.
Yahya, Mohd Danial. "Immunological studies of malondialdehyde in a murine lupus model." Diss., The University of Arizona, 1992. http://hdl.handle.net/10150/185941.
Laprano, Nicola. "Metabolic alterations in a murine model of Barth syndrome." Thesis, University of Glasgow, 2018. http://theses.gla.ac.uk/9105/.
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Dissertação (mestrado) - Universidade Estadual de Campinas, Faculdade de Ciências Médicas
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Resumo: Introdução e hipótese: O implante de slings corresponde ao padrão atual de tratamento da incontinência urinária de esforço. Para sua eficácia, exigem um mecanismo de ancoragem adequado. O objetivo deste estudo foi comparar as características biomecânicas de sistemas de fixação de dois modelos de mini slings atualmente disponíveis no mercado (Ophira® e Mini Arc®) por meio de teste de tração uniaxial em modelo murino ex vivo. Materiais e Métodos: Os dispositivos de ancoragem dos mini sling foram implantados cirurgicamente na parede abdominal de 15 ratos divididos em três grupos de cinco animais. Estes grupos foram sacrificados de acordo com a data pós implante em 7, 14 e 30 dias. Na sequência, extraia-se em bloco a parede abdominal de cada animal contendo os dispositivos de fixação. Este material era submetido a teste de tração até o despreendimento do sistema de ancoragem a partir do tecido leito de implante. Foram determinadas a medida da carga máxima (N) e deformação total (mm) até a avulsão. Os resultados foram comparados por meio do teste t de Student e o valor de p 5% foi considerado significativo. Resultados: O sistema de fixação do mini sling Ophira® em comparação ao Mini Arc® exigiu maior carga máxima para avulsão do tecido abdominal nos 3 períodos avaliados com significância estatística: 7º dia, 5,14 ± 0,69 N versus 4,11 ± 0,81 N (p 0,037), 14º dia 10,64 ± 0,81 N contra 9,02 ± 0,54 N (p 0,001) e no 30º dia 18,76 ± 1,48 N versus 14,85 ± 1,28 N (p < 0,001), respectivamente. Quanto ao deslocamento ocorrido durante a tração, o Ophira® desenvolveu maior deflexão significante em todos os momentos avaliados: no dia 7, 11,14 ± 0,53 mm enquanto o Mini Arc® 7,89 ± 1,01 mm (p 0,001), no dia 14 15,80 ± 0,95 mm versus 12,49 ± 1,43 mm (p < 0,001) e no trigésimo dia os valores foram 23,95 ± 1,38 mm contra 18,24 ± 0,50 mm (p < 0,001), respectivamente. Conclusão: O dispositivo de ancoragem do mini sling Ophira® proporcionou melhor fixação à parede abdominal murina em relação ao do Mini Arc®. Este resultado foi mantido mesmo no período pós-operatório tardio
Abstract: Introduction and hypothesis: Slings are currently the standard treatment for stress urinary incontinence. To be effective, they require an adequate anchoring system. The aim of this study is compare the biomechanical features of fixation systems of two mini slings models currently available on the market (Ophira¿ and Mini Arc¿). Materials and Methods: Anchoring devices of each sling were surgically implanted in the abdominal wall of 15 rats divided into three groups of five animals. These groups are formed according to the date of post implant euthanasia on 7, 14 and 30 days. Each abdominal wall was extracted in block containing the mini sling fixation device and was submitted to a tensile strength test for measure the maximum load and total deflection until device avulsion from the tissue. The results were compared using Student test t and a 5% cut off was considered significant. Results: The Ophira¿ mini sling fixation system demanded a greater maximum load compared to the Mini Arc¿ for avulsion of abdominal tissue in 3 evaluation periods with statistical significance: 7th day, 5.14 ± 0.69 versus 4.11 ± N 0,81 N (p 0.037), on day 14 10.64 ± 0.81 N versus 9.02 ± 0.54 N (p 0.001) and on day 30 18.76 ± 1.48 N versus 14.85 ± 1.28 N (p <0.001), respectively. Regarding the displacement occurred during traction, the Ophira¿ has developed significant higher deflection at all times evaluated: on day 7, 11.14 ± 0.53 mm while the Mini Arc¿ 7.89 ± 1.01 mm (p 0.001), on day 14 15.80 ± 0.95 versus 12.49 mm ± 1.43 mm (p <0.001) and on day 30 the values were 23.95 ± 1.38 mm vs. 18.24 ± 0.50 mm (p <0.001), respectively. Conclusion: The Ophira¿ mini sling anchoring device provided better fixation to the host tissue site compared to the Mini Arc¿ device in rats. This outcome were maintained even in the late post operative period
Mestrado
Fisiopatologia Cirúrgica
Mestre em Ciências