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Academic literature on the topic 'Murashige and Skoog'

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Published: 9 March 2023
Last updated: 10 March 2023

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Journal articles on the topic "Murashige and Skoog"

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Srilestari, Rina, and Suwardi Suwardi. "INDUCTION OF ABACA BANANA ROOTS BY IN VITRO USING KINDS OF MEDIA AND THIAMIN." Agrivet 26, no. 1 (January 9, 2021): 1. http://dx.doi.org/10.31315/agrivet.v26i1.4304.

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Abaca is a type a banana with high economic value with it stem fiber used in textile and paper industries. As a superior commodity, its number is relatively limited, with the need of a largeplanting area to meet the high market demand. The aim of the research was to observe the abaca banana explants response to various media and Thiamin. The experiment was done at Biotechnology laboratory, UPN “Veteran” Yogyakarta. Treatments were arranged in completely randomized design with 2 factor. The first factor is the growing media: Murashige & Skoog, a half Murashige & Skoog media, Vacint & Went Media and the second factor is the Thiamin concentration: 2 mg/L; 3 mg/L; 4 mg/mL.The results showed there is an interactions on the parameters of planlet height, number of lenghth of root in the combination of Murashige and Skoog and thiamin 3 mg/L medium. Murashige and Skoog medium produced the highest fresh weight and dry weight and Thiamin concentration 3 mg/L produced fresh and dry weight in the highestKey words: abaka, root induction, various media, thiamin
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Arfa, Nadia Fakhriyati, Endang Nurcahyani, Zulkifli Zulkifli, and Tundjung Tripeni Handayani. "Nepenthes mirabilis (Lour.) Druce Planlet at a Various Levels of Murashige & Skoog Medium Density In Vitro." Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati 6, no. 2 (December 1, 2019): 18–22. http://dx.doi.org/10.23960/jbekh.v6i2.25.

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This study aims to determine the variation of the stomata index of the Kantong Semar (Nepenthes mirabilis) planlet at a various medium density of the Murashige and Skoog. This study used a Completely Randomized Design using one factor (medium density of the Murashige and Skoog). We used 5 levels of medium density, i.e.: 1/16 MS, 1/8 MS, 1/4 MS, 1/2 MS, and MS. Homogeneity test used Levene’s test of 5% significance level, then analysis of variance is carried out at 5% significance level and followed by Tukey test at 5% significance level. The results showed that the lower the level of Murashige and Skoog medium density on the Nepenthes mirabilis plantlet, the stomata index also increased.
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Shahriyar, Sayeed, Soleh Akram, Koushik Khan, Md Faruk Miya, and Md Abdur Rauf Sarkar. "In vitro plant regeneration of potato (Solanum tuberosum L.) at the rate of different hormonal concentration." Asian Journal of Medical and Biological Research 1, no. 2 (November 23, 2015): 297–303. http://dx.doi.org/10.3329/ajmbr.v1i2.25625.

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One of the goals of the experiment is to standardization of HgCl2 treatment for explants sterilization. The objectives also include developing a reproducible cost effective protocol for large scale production of Solanum tuberosum of Cardinal variety plantlets from selectively better clones through plant in vitro propagation methods. Selection of growth regulators for proper multiple shoots regeneration, elongation and root induction. To produce genetically uniform plantlets within a short time capable surviving in natural condition raised in in vitro environment. Shoot tip and nodal segment explants from field grown plants were used as experimental materials in this investigation. All explants were cultured on Murashige and Skoog medium supplemented with various plant growth regulators. For surface sterilization of explants, HgCl2 (0.1%) for 2 minutes was found to be most effective for complete destroying of surface pathogens and getting healthy tissues. Shoot regeneration was observed from both shoot tips and nodal explants for the studied plant. Maximum number of shoot per culture (17) was recorded and it also obtained the highest average length of the shoot (5cm) in Murashige and Skoog medium containing no hormone. On the other hand 6-benzyl amino purine (0.2mg/l) in 3 media showed the highest rate of shoot multiplication (73%) and the highest average length (4cm). In case of Gibberellic acid (0.1mg/l) in Murashige and Skoog media showed its highest rate of shoot regeneration (82%) and the highest average length (4.5cm). From the overall experiment it was observed that shoot tips are more responsive for micro propagation. In root induction Murashige and Skoog medium supplemented with different concentration (0.5, 1, 1.5 and 2mg/l) of indol-3-acetic acid and kinetin. Indol-3-acetic acid and kinetin (1.5+1.5 mg/l) showed its lowest rate of root regeneration (40%) and the average length of the root (1.5 cm). On the contrary Murashige and Skoog medium with no hormone showed the rate of root regeneration (96%) and the highest average length of the root (2.5 cm). The supplemented Murashige and Skoog media with no hormone showed the best performance for root regeneration.Asian J. Med. Biol. Res. June 2015, 1(2): 297-303
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Mederos, Sebastiana, Lucía San Andrés, and Javier G. Luis. "Rosmanol controls explants browing of Hypericum canariensis L. during the in vitro estabilishment of shoots." Acta Societatis Botanicorum Poloniae 66, no. 3-4 (2014): 347–49. http://dx.doi.org/10.5586/asbp.1997.042.

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An efficient method for eradication of browning exudate was developed for <em>Hypericum canariensis</em>. For this purpose the effect of natural products on browning exudates were investigated in four types of culture media: Murashige and Skoog (MS, 1962); Gamborg's (B5, 1979); Woody Plant Medium (WPM, Lloyd and McCown 1981) and modified Quoirin and Lepoivre (QL.4) (Mederos 1991, Mederos et al. 1995) basal macroelements; these basal macroelements were supplemented with the microelement formula described by Murashige and Skoog (MS, 1962). During the establishment of shoots organogenesis potential was achieved in the Murashige and Skoog (MS, 1962) and modified Quoirin and Lepoivre (QL. 4) (Mederos 1991, Mederos et al. 1995) media after browning exudates was eliminated by rosmanol treatments. Rosmanol is a powerful diterpenic antioxidant isolated from <em>Salvia canariensis</em> L., a medicinal species endemic to the Canary Islands.
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Flinn, Barry S., David T. Webb, and Wanda Georgis. "In vitro control of caulogenesis by growth regulators and media components in embryonic explants of eastern white pine (Pinus strobus)." Canadian Journal of Botany 64, no. 9 (September 1, 1986): 1948–56. http://dx.doi.org/10.1139/b86-258.

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Horizontally oriented embryos of Pinus strobus (L.) produced shoots on Schenk and Hildebrandt medium containing cytokinin. Shoots developed primarily from cotyledons in contact with the medium. Seed pretreatments at 5 or 27 °C did not affect caulogenesis. N6-Benzyladenine (BA) and N6-(Δ2-isopentenyl)adenine (2iP) both induced caulogenesis, with BA being 10–20 times more potent than 2iP. High BA levels caused callus formation. BA exposures from 1 to 8 weeks were equally caulogenic with horizontal explants, but exposures longer than 2 weeks led to increased variability and callus formation. A 1-week, upside-down, vertical orientation during BA treatment increased the uniformity of cotyledon response and was as caulogenic as a 4-week horizontal BA exposure. Neither auxins nor triiodobenzoic acid induced or significantly enhanced shoot formation. Full-strength Schenk and Hildebrandt medium was superior to Murashige and Skoog medium for shoot induction. Dilution of Schenk and Hildebrandt medium had no significant effect on shoot production, but shoot elongation was suppressed on one quarter strength Schenk and Hildebrandt medium. Half-strength Murashige and Skoog medium was as caulogenic as Schenk and Hildebrandt medium. The NH4 level of the macronutrients was responsible for the difference between Schenk and Hildebrant medium and Murashige and Skoog medium. The higher NH4 concentration of Murashige and Skoog medium inhibited shoot formation.
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Das, P. "Mass cloning of Rose and Mussaenda, popular garden plants, via somatic embryogenesis." Horticultural Science 37, No. 2 (May 6, 2010): 70–78. http://dx.doi.org/10.17221/57/2009-hortsci.

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Protocols were developed for propagation of Rosa hybrida cv. Landora and Mussaenda erythrophylla cv. Rosea via somatic embryogenesis by manipulating growth regulators and culture conditions. Calli were induced from young leaf explants of Rosa hybrida cv. Landora and Mussaenda erythrophylla cv. Rosea on Murashige, Skoog medium supplemented with 6-benzylaminopurine or kinetin along with indole-3-acetic acid or 2,4-dichloroacetic acid within four weeks of culture. The calli were subcultured either in the same medium or in a modified medium for induction of embryogenic callus. Embryogenic calli in rose were developed on Murashige, Skoog medium supplemented with 0.5&ndash;1.0 mg/l 6-benzylaminopurine, 2.0 mg/l 2,4-dichloroacetic acid, and 400&ndash;800 mg/l l-proline or l-glutamine. The results showed that stimulation of auxin-induced somatic embryogenesis by proline has a great impact on development of somatic embryos and secondary somatic embryogenesis in rose. In Mussaenda, embryogenic calli were developed on Murashige, Skoog medium supplemented with 0.5&ndash;1.0 mg/l 6-benzylaminopurine, 2.0&ndash;3.0 mg/l indole-3-acetic acid, and 10 mg/l ascorbic acid. Somatic embryos were isolated and transferred to half-strength Murashige, Skoog medium supplemented with 0.25&ndash;0.5 mg/l 6-benzylaminopurine + 0.1 mg/l gibberelic acid + 5.0 mg/l adenine sulfate and 2% sucrose for maturation and germination. About 70% somatic embryos of Mussaenda germinated. The rose somatic embryos, however, did not germinate. The somatic embryos of rose, when incubated in the dark at 4&deg;C for two weeks and transferred to 1/2 strength Murashige, Skoog medium supplemented with 0.5 mg/l 6-benzylaminopurine, 0.25 mg/l gibberelic acid, and 2% sucrose, showed 60% germination. The seedlings showed a distinct shoot development but the radicles were blunt without well-defined root system. The shoots were harvested and cultured in the multiplication medium containing Murashige, Skoog medium supplemented with 1.0 mg/l 6-benzylaminopurine and 0.1 mg/l indole-3-acetic acid for four weeks and then subcultured in the same medium for further multiplication. The somatic embryos of Mussaenda erythrophylla cv. Rosea germinated into normal plantlets with distinct shoot and well-developed root system. The somatic embryo-derived plantlets grew normally and flowered within two months of transfer to the field.
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Mpika, Joseph, Chanelle Bertille Mvoumbi Tete, Chrichina Mbon Nguekou, Blaise Pascal Ngondo, Laurine Valerie Mboutol-Mandavo, and Attibayeba a. "ORGANOGENESE IN VITRO DES FRAGMENTS DE TUBERCULES DE POMME DE TERRE (SOLANUM TUBEROSUM L. VAR. AIDA) CULTIVES DANS LE DEPARTEMENT DES PLATEAUX (REPUBLIQUE DU CONGO)." International Journal of Advanced Research 10, no. 08 (August 31, 2022): 1065–73. http://dx.doi.org/10.21474/ijar01/15270.

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The production of potatoes rich in starch and essential nutrients does not cover the needs of Congolese consumers. This is due to strong genetic erosion and the absence of high-performance local varieties as well as the unavailability of seeds for new plantations. The seed produced by traditional techniques does not meet the demand of producers. This study aims to produce seed potatoes by the technique of in vitro culture. Tubers disinfected with 10% sodium hypochlorite are kept at 4°C for 20 days to produce microtubers by bud burst others are placed in heat therapy conditions to produce micro-cuttings. Microtubers and microcuttings are taken, then cultured in vitro on Murashige and Skoog medium on the one hand and on Murashige and Skoog medium to which 0.05 mg/ml of indolylbutyric acid is added on the other hand. The cold favors the bud burst of the potato tubers after a week. On vitroplants from microtubers developing on Murashige and Skoog medium to which 0.05 mg / ml of indolylbutyric acid is added, 5 roots, 6 leaves and a stem with a height of 6.05 cm are recorded. On the medium of Murashige and Skoog, we observe 3 roots, 7 leaves and a stem 5.69 cm in height. Regarding microprogation, the results obtained showed that growth hormone plays an important role in the different stages of regeneration compared to the medium without phytohormone.
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Martini, Priscilla Cavalcante, Lilia Willadino, Gilberto Dias Alves, and Virgínia Maria Tenório Sabino Donato. "Propagação de orquídea Gongora quinquenervis por semeadura in vitro." Pesquisa Agropecuária Brasileira 36, no. 10 (October 2001): 1319–24. http://dx.doi.org/10.1590/s0100-204x2001001000015.

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O objetivo deste trabalho foi avaliar a germinação e a organogênese in vitro em Gongora quinquenervis em dois meios nutritivos, Knudson "C" e Murashige & Skoog, com três concentrações de BAP (0,0, 0,5 e 1,0 mg L-1). Os protocormos cultivados no meio Knudson "C" necrosaram. A maioria dos embriões cultivados em meio Murashige & Skoog tendeu a diferenciar-se em calos. Estes calos apresentaram alto potencial morfogenético, regenerando grande número de plantas via organogênese indireta, sobretudo no material proveniente do tratamento desprovido de BAP. Foram formadas 41 plantas pela rota normal de germinação, contrastando com 715 plantas regeneradas via organogênese indireta.
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Abdelazeez, Walla Abdelmaksood, Landysh Zavdetovna Khusnetdinova, and Olga Arnoldovna Timofeeva. "Future outlook of plant growth regulators application influencing callus induction from different type explant Hyoscyamus muticus L. in vitro." Samara Journal of Science 7, no. 3 (August 15, 2018): 10–13. http://dx.doi.org/10.17816/snv201873101.

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The article shows the results concerning the problem of the influence of the hormonal composition of the medium on callus induction in isolated from different explants of Egyptian henbane areas (on the example of Hyoscyamus muticus L.). The authors study 11 variants of Murashige and Skoog medium supplemented with different concentrations and combination of auxins and cytokinins. It was important to find nutrient medium modification of Murashige and Skoog for callus induction. The article describes the fact that callus formation from different explant types of Hyoscyamus muticus L. in vitro was observed on Murashige and Skoog medium fortified with benzylaminopurine and naphthylacetic acid. It shows that the maximum callus induction was observed from root explants on Murashige and Skoog's medium supplemented with 0.5 mg/l of benzylaminopurine and 1.0 mg/l of naphthylacetic acid. And minimal callus formation was observed in the area with benzylaminopurine. Callus induction of leaf and stem explants both on the hormone-free nutrient medium and with the benzylaminopurine only was not observed. Thus, the results show that the frequency of callus formation with culturing root segment is higher compared to leaf and stem segment explants (on the example of Egyptian henbane in culture in vitro ). This work aims to inducing callus formation from various explants of Egyptian henbane, which can be used for plant regeneration or as a source for in vitro production of secondary metabolites.
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López-Puc, Guadalupe, Adriana Canto-Flick, Felipe Barredo-Pool, Patricia Zapata-Castillo, María del C. Montalvo-Peniche, Felipe Barahona-Pérez, Nancy Santana-Buzzy, and Lourdes Iglesias-Andreu. "Direct Somatic Embryogenesis: A Highly Efficient Protocol for In Vitro Regeneration of Habanero Pepper (Capsicum chinense Jacq.)." HortScience 41, no. 7 (December 2006): 1645–50. http://dx.doi.org/10.21273/hortsci.41.7.1645.

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To induce somatic embryogenesis in habanero pepper (Capsicum chinense Jacq.), the cultivar BVll-03, belonging to the red type, was used. Different explants were evaluated, as were different culture media, the composition of which varied in the content of plant growth regulators. Results showed the formation of somatic embryos from cotyledons, zygotic embryos, germinated zygotic embryos, hypocotyls, and cotyledonary leaves. Explants were cultured on Murashige and Skoog medium supplemented with 2,4-D (9.05 μm). The somatic embryos always formed directly from the explant, without callus formation, and the greatest efficiency was obtained when segments of hypocotyls were cultured, obtaining 175 ± 20 somatic embryos per explant. Only the somatic embryos obtained on Murashige and Skoog medium containing 2,4-D (9.05 μm) and treated with abscisic acid (ABA) (1.89 μm) before their transfer to the germination media (Murashige and Skoog + 1.1 μm GA3) emitted their radicule and expanded their cotyledonary leaves (60%), whereas the remaining embryos did not achieve germination because of different causes (abnormalities, delayed development). Not only is this protocol of somatic embryogenesis the first to be reported for this species (C. chinense Jacq.), but it is also the most efficient reported so far, within the Capsicum genus.
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Dissertations / Theses on the topic "Murashige and Skoog"

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Alejos, Marcos. "Transient Expression of BABY BOOM, WUSCHEL, and SHOOT MERISTEMLESS from Virus-Based Vectors in Cotton Explants: Can We Accelerate Somatic Embryogenesis to Improve Transformation Efficiency?" Thesis, University of North Texas, 2019. https://digital.library.unt.edu/ark:/67531/metadc1609070/.

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Upland cotton (Gossypium hirsutum L.) is the world's most prominent fiber crop. Cotton transformation is labor intensive and time consuming, taking 12 to 18 months for rooted T0 plants. One rate limiting step is the necessary production of somatic embryos. In other recalcitrant species, ectopic expression of three genes were shown to promote somatic embryogenesis: WUSCHEL (WUS), SHOOT MERISTEMLESS (STM), and BABY BOOM (BBM). WUS is responsible for maintaining stem-cell fate in shoot and floral meristems. STM is needed to establish and maintain shoot meristems. STM and WUS have similar functions but work in different pathways; overexpression of both together converts somatic cells to meristematic and embryogenic fate. BBM encodes an AP2/ERF transcription factor that is expressed during embryogenesis and ectopic expression of BBM reprograms vegetative tissues to embryonic growth. In prior studies, these genes were constitutively expressed, and cultures did not progress beyond embryogenesis because the embryogenic signal was not turned off. In our study, we set out to use these genes to increase the efficiency of cotton transformation and decrease the time it takes to regenerate a plant. A disarmed cotton leaf crumple virus (dCLCrV) vector delivers WUS, STM, or BBM into cotton tissue cultures through Agrobacterium tumefaciens infection. We propose that virus delivery of embryo-inducing genes is a better approach for transformation because A) inserts more than 800 nucleotides are unstable, and will spontaneously inactivate, B) virus DNA can migrate through plasmodesmata to cells around the infected cell, creating a gradient of embryonic potential, C) the virus DNA does not pass through the germ line and the seed will not contain virus. We propose this method of inducing embryogenesis will facilitate the stable transformation of cotton and will be beneficial to the cotton industry. Ectopic expression of AtBBM, AtSTM, and AtWUS GrWUS:meGFP from a constitutive CaMV 35S promoter produced plants with phenotypes similar to those described in previous studies overexpressing AtBBM, indicating that the AtBBM gene was functional. The cotton cotyledon infiltration of the pART27 constructs showed transformed cells in Coker 312 by GFP localization in the nucleus. Although GFP was detected, no visible embryos appeared from the cotyledon. Cotyledons infiltrated with Agrobacterium harboring overexpression vectors withered and aborted after ~2 weeks. The virus-based vector in tissue culture failed to increase transformation efficiency, resulting in no embryos. The combination of hormone concentration showed no contribution to increasing the transformation efficiency.
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CARAMANICO, LEILA. "STUDY OF GRAPEVINE ROOTSTOCK RESPONSE TO WATER STRESS." Doctoral thesis, Università degli Studi di Milano, 2020. http://hdl.handle.net/2434/707586.

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More than 80% of vineyards around the world use grafted plants: a scion of Vitis vinifera grafted onto a rootstock of single or interspecific hybrids of American Vitis species, resistant or partially resistant to Phylloxera (Daktulosphaira vitifoliae (Fitch, 1856)). The genetic variability of grapevine rootstocks plays a fundamental role in their adaptation to the environment (Serra et al., 2013). In the climate change scenario, predicting an increase of aridity in the near future (Dai, 2013), the more frequent and severe drought events may represent the major constrain for the future of viticulture (IPCC, 2018; Schultz, 2000). Therefore, the selection of new rootstocks able to cope with unfavourable environmental condition is a key asset, as well as a strategy to improve crop yield/vegetative growth balance on scion behaviour (Corso and Bonghi, 2014). So far, the influence of rootstock on scion physiological performance during water stress has always aroused great interest. On the contrary, the scion impact on rootstock response is still less debated. Therefore, the effect of grafting on rootstock behaviour have been investigated. Phenotypical and large-scale whole transcriptome analyses on two genotypes, a drought-susceptible (101-14) and a drought-tolerant (1103 P), own-rooted and grafted with Cabernet Sauvignon, subjected to a gradual water shortage in semi-controlled environmental conditions have been performed. The ungrafted condition affected photosynthesis and transpiration, meaning the decisive role of scion in modulation of gas exchanges and in general in plant adaptation. Molecular evidence highlighted that the scion delays the stimulus perception and rootstock reactivity to drought. Since 1985, the DiSAA research group operating at the University of Milan is carrying on a rootstock crossbreeding program which has led to the release of four genotypes: M1, M2, M3 and M4. They show from moderate to high tolerance to drought (M4 > M1 = M3 > M2). In order to characterize their performance during water stress, their physiological (gas exchanges and stem water potential) and transcriptome response (genes involved in ABA-synthesis and ABA-mediated responses to drought) under well-watered and water stress conditions were examined. The behaviour of M-rootstocks (M1, M2 and M3) was compared with that of other commercial genotypes largely used in viticulture, either tolerant (140 Ru, 41 B, 110 R, 1103 P), less tolerant (SO 4, K 5BB) and susceptible (420 A and Schwarzman). Discriminant analysis (DA) showed that when water availability starts to decrease, rootstocks firstly perceives the stress activating a transcriptome response, consequently physiological changes have been observed. It also demonstrated that the three M-rootstocks were clearly discriminated: M4 was grouped with the most tolerant genotypes while M3 with the less tolerant or susceptible ones from a physiological standpoint, confirming their different attitude to tolerate water stress. M4 has proven to be a promising rootstock due to its ability to adapt to drought conditions. Considering the constant great demand for vine planting materials, the obtainment of genetically homogeneous populations (i.e. clones) from elite individuals through micropropagation represents a rapid alternative to conventional multiplication. For this reason, an efficient high-throughput protocol for M4 in vitro propagation was set up. Its attitude to shooting, root development and callus proliferation was compared to that of other rootstocks largely used in viticulture (K5BB, 1103P, 101-14 and 3309C). Moreover, pro-embryogenic and embryogenic callus from bud explants were also produced, representing a cellular material manipulable with the genetic engineering techniques. In water scarcity condition, among the mechanisms activated by M4, the great ability to scavenge ROS, related to the increased accumulation of stilbenes and flavonoids, may be such as to give it tolerance to the stress. In particular, the higher levels of trans-resveratrol were correlated with the up-regulation of some stilbene synthase genes, mainly VvSTS16, VvSTS18, VvSTS27 and VvSTS29. The over expression of these genes was linked to a structural variation in their promoter region. To confirm that VvSTSs genes may be considered putative factors of M4 better adaptation to water stress, a genome editing protocol based on the CRISPR/Cas9 system, aimed at knock-out the genes, was performed. For testing the gRNAs functionality, a transient assay on in vitro micropropagated plantlets of M4 and 101-14 was performed. The positive results obtained by this experiment will lead to the transformation of somatic embryos and regeneration of whole-edited plants using the vectors developed.
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Books on the topic "Murashige and Skoog"

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Sukhaphat, Čharūn. Kānsưksā prīapthīap kānčharœ̄n khō̜ng klūaimai ʻƯ̄ang King Dam (Dendrobium gratiosissimum Rchb.f.) nai ʻāhān sūt datplǣng mūrāchike læ sakūk (1962) =: A comparative study of the growth rate of Dendrobium gratiosissimum Rchb.f. in sterilized cultures using a modified version of the murashige and skoog (1962). [Nakhon Sawan]: Khana Witthayāsāt læ Thēknōlōyī, Mahāwitthayālai Rātchaphat Nakhō̜n Sawan, 2008.

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Book chapters on the topic "Murashige and Skoog"

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"Murashige & Skoog Medium (MS1)." In Encyclopedia of Genetics, Genomics, Proteomics and Informatics, 1290. Dordrecht: Springer Netherlands, 2008. http://dx.doi.org/10.1007/978-1-4020-6754-9_10971.

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Çördük, Nurşen, and Cüneyt Aki. "In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey." In Endangered Plants [Working Title]. IntechOpen, 2020. http://dx.doi.org/10.5772/intechopen.94378.

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Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.
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JOÃO ELIÉZER DE SOUZA, BATISTA, MANTOVANI CIBELE, FERREIRA KÁSSIA BARROS, SOUZA ANTONIO MARICÉLIO BORGES DE, and PIVETTA KATHIA FERNANDES LOPES. ": SILÍCIO NO CRESCIMENTO E DESENVOLVIMENTO IN VITRO E NA ACLIMATIZAÇÃO DE Cattleya amethystoglossa LINDEN & RCHB.F. (ORCHIDACEAE)." In PERSPECTIVAS DAS CIÊNCIAS AGRÁRIAS NA SOCIEDADE 5.0: EDUCAÇÃO, CIÊNCIA, TECNOLOGIA E AMOR, 328–34. Instituto Internacional Despertando Vocações, 2020. http://dx.doi.org/10.31692/978-65-88970-07-2.328-334.

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As plantas ornamentais são reconhecidamente capazes de proporcionar bem-estar e satisfação àqueles que as contemplam; dentre elas, as orquídeas se destacam como mais apreciadas e de maior valor comercial (LORENZI e SOUZA, 2001). Cattleya amethystoglossa Linden & Rchb.f. é uma orquídea endêmica do Brasil, muitíssimo bela, que ocorre com maior frequência no estado da Bahia, mas também no Espírito Santo e Minas Gerais. (CATTLEYA, 2019). O cultivo in vitro têm auxiliado na preservação de várias espécies vegetais, dentre elas as orquídeas. Esta técnica possibilita o manuseio de grande número de indivíduos em espaço reduzido, com controle dos fatores do ambiente e sob condições assépticas, estimulando o crescimento, e assim adquirindo o controle sobre o cultivo dessas espécies. Estudos que tenham como finalidade dominar os processos de propagação das orquídeas tornam-se extremamente importantes para que seja possível viabilizar a sua multiplicação em coleções vivas e possibilitar tanto a reintrodução na natureza como também a conservação daquelas espécies ameaçadas de extinção (FERREIRA e SUZUKI, 2008). A aplicação de silício tem sido reportada por aumentar o crescimento e o desenvolvimento de várias plantas, portanto, é recomendada a sua inclusão como elemento benéfico no meio de cultura de tecidos para resolver vários problemas na micropropagação e para aumentar o sucesso da cultura de tecidos (SIVANESAN e PARK, 2014). Desta forma, esta pesquisa teve como objetivo, estudar os efeitos da suplementação do meio de cultivo Murashige e Skoog com ácido monossilícico, no crescimento e desenvolvimento in vitro e na aclimatização de plântulas da orquídea Cattleya amethystoglossa Linden & Rchb.f..
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Conference papers on the topic "Murashige and Skoog"

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Taipova, R. M., and B. R. Kuluev. "Agrobacterium-mediated transformation of Amaranthus cruentus L. epicotyls by the ARGOS-LIKE transgene." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.244.

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The present study describes the results of our research in Agrobacterium-mediated transformation of epicotyl segments of Amaranthus cruentus variety “Bagryanyi” by the ARGOS-LIKE transgene of Arabidopsis thaliana controlled by the 35S promoter. For shoot regeneration from epicotyl segments after Agrobacterium-mediated transformation, Murashige-Skoog (MS) medium containing 13 μM 6-benzylaminopurine and 1 μM α-naphthylacetic acid was used. For the selection of transgenic shoots, 10 mg/L of hygromycin B was added to the MS medium.
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Mukhamatdinova, E. A., I. S. Kovtun, and M. V. Efimova. "The regulation of growth parameters of potato microclones by jasmonic acid." In 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology". PLAMIC2020 Organizing committee, 2020. http://dx.doi.org/10.28983/plamic2020.175.

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The microclones of potato (variety Lugovskoy) were grown on the modified Murashige-Skoog (MS) agar medium in the absence (control) or presence of JA at concentrations of 0.001, 0.1, and 10 µM. We evaluated plant growth parameters such as the length of the axial organs, the number of stolons, leaves, the area of the assimilating surface, and the wet and dry mass of aboveground and underground organs. For the first time, has been demonstrated, that jasmonic acid (0.1 and 10 µM) was showed a pronounced growth-stimulating effect on potato plants.
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Momčilović, Jovana, Dragana Jakovljević, Milica Kanjevac, and Biljana Bojović. "FIZIOLOŠKE KARAKTERISTIKE RASTENJA PŠENICE (Triticum aestivum L.) U USLOVIMA IN VITRO." In XXVII savetovanje o biotehnologiji. University of Kragujevac, Faculty of Agronomy, 2022. http://dx.doi.org/10.46793/sbt27.503m.

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This study aimed to examine the effect of different media - Murashige Skoog (MS) and Gamborg (B5) on the growth and development of in vitro seedling cultures of wheat (Triticum aestivum L.). The effects were evaluated through the measurement of root and shoot length, fresh and dry mass, as well as through the determination of the concentration of photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoids). The obtained data indicate that MS has better effects on the growth and development of wheat seedlings, since longer shoot length, and better fresh weight were observed on seedlings from this type of media. Additionally, higher chlorophyll b concentration and lower carotenoid concentration were measured in wheat leaves grown on MS medium. It can be concluded that MS is more suitable for establishing the initial in vitro culture of wheat compared to the B5 medium.
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Shikunets, D. D., and T. A. Krasinskaya. "EFFICIENCY OF REGENERATION PROCESSES OF EXPLANTS OF GRAPE VARIETY MARQUETTE AT THE INTRODUCTION STAGE IN VITRO CULTURE AND STABILIZATION OF STERILE CULTURE." In SAKHAROV READINGS 2022: ENVIRONMENTAL PROBLEMS OF THE XXI CENTURY. International Sakharov Environmental Institute of Belarusian State University, 2022. http://dx.doi.org/10.46646/sakh-2022-1-282-285.

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The regenerative plants of the Marquette variety, obtained during the research, were included in the collection of the genebank of the RUE «Institute of Fruit Growing», kept under in vitro conditions. The use of a sterilizing scheme for green internodes of grapes, including 30% hydrogen peroxide with an exposure of 7 minutes and their cultivation on a modified Murashige-Skoog nutrient medium containing 1.1 mg/l 6-BA, gave the maximum proportion of sterile and viable explants (85.0% ). A significant aftereffect on the stabilization processes of regenerated plants during further cultivation was noted: regenerated plants of the Marquette variety, the introduction of explants of which was carried out on MS medium containing 1.1 mg/l 6-BA, using 30% H2O2 with an exposure of 7 minutes, had a great potential to the laying of new microplants (multiplication factor was 3.15) and a smaller share of callus formation at the base of plants (18.0%).
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Ariyanti, Nur Khofifah, Dyah Nuning Erawati, Rahma Sarita, and Sabilla Jasmine Belinda. "Analisis Peran Air Kelapa Terhadap Pertumbuhan Eksplan Kultur Vanili (Vanilla planifolia)." In Seminar Nasional Semanis Tani Polije 2021. Politeknik Negeri Jember, 2021. http://dx.doi.org/10.25047/agropross.2021.210.

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Prospek vanili (Vanilla planifolia) sebagai komoditas penghasil devisa negara masih sangat berpeluang untuk dikembangkan. Salah satu teknik dalam pengembangan vanili melalui metode budidaya kultur jaringan tanaman dengan penambahan air kelapa sebagai sumber zat pengatur tumbuh alami agar eksplan asal ruas batang vanili dapat tumbuh dan berkembang secara optimal. Air kelapa dapat diperoleh dengan mudah sehingga lebih efisien pada segi biaya dan waktu. Oleh karena itu, tujuan dari penelitian ini adalah menganalisis peran air kelapa terhadap pertumbuhan eksplan kultur vanili. Pelaksanaan penelitian pada bulan Februari - Juni 2021 di Laboratorium Kultur Jaringan Politeknik Negeri Jember. Metode analisis T test digunakan untuk membandingkan perlakuan tanpa penambahan air kelapa (P0) dengan perlakuan penambahan air kelapa 15% (P1) pada media dasar Murashige-Skoog. Hasil penelitian memperlihatkan bahwa 1) Penambahan air kelapa 15% mempengaruhi tinggi tunas eksplan vanili dengan rerata 4,45 cm/eksplan dan jumlah akar dengan rerata 2,03 akar/eksplan pada umur eksplan 56 hsi, 2) Penambahan air kelapa 15% memiliki pengaruh yang sama dengan tanpa penambahan air kelapa terhadap kedinian bertunas, jumlah akar pada 14, 28, 42 hsi dan panjang akar eksplan vanili, 3) Penambahan air kelapa 15% mampu mendukung pertumbuhan panjang tunas dan akar tetapi tidak mampu menggandakan tunas eksplan vanili.
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"Effect of Murashige and Skoog Salts Strength Medium (MS) on Steroids Production and Total Amino Acids Content of Date Palm Embryonic Callus (Sakkoty and Bartamuda cultivar)." In By-Products of Palm Trees and Their Applications. Materials Research Forum LLC, 2019. http://dx.doi.org/10.21741/9781644900178-18.

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Sasco, Elena. "Efectele genetice implicate în răspunsul grăului comun la filtratul de cultură Drechslera sorokiniana (SACC.) subram." In VIIth International Scientific Conference “Genetics, Physiology and Plant Breeding”. Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2021. http://dx.doi.org/10.53040/gppb7.2021.71.

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Helminthosporiosis caused by the fungus Drechslera sorokiniana (Sacc.) causes significant crop and quality losses to Triticum aestivum L. in agroecological conditions with extreme humidity. Increasing the resistance is considered the most cost-effective and sustainable approach to disease control. The aim of this study was to determine the genetic effects involved in the inheritance of resistance, using the ge-netic model of character reproduction in descendants of wheat. Generations F1, F2, BCP1 and BCP2, de-scended from the mutual crossing of the parents Basarabeanca / Moldova 30 and Moldova 30 / Moldova 3 (P1 and P2) were evaluated for the response of callus characters to the action of D. sorokiniana culture filtrate on the medium Murashige Skoog. Fungal metabolites have decreased the effects of gene actions and epistatic interactions, but also their variance. The phenomenon corresponds to the decrease of callus indices. A great importance for the heredity of the character of the surface of the callus manifested the epistatic effects of additive-dominant (ad) type. In the case of callus biomass comparable to the mean val-ues were the a actions, but also the ad and dd epistatic effects. The predominant involvement of epistatic effects indicates the need for resistance selections to helminthosporiosis in late generations of wheat.
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VIEIRA, MUZA DO CARMO, KERLY CRISTINA PEREIRA; GILSON DOURADO DA SILVA, LíDIA MARIA MORAIS MONTEIRO LAMIM; THIMóTEO PAES BARBOSA, MARIANA SILVA PEREIRA DE PAULA, ANDERSON DIAS VAZ DE SOUZA, and JANAINA BORGES DE AZEVEDO FRANçA. "Estabelecimento e Desenvolvimento Inicial In Vitro de Baruzeiro (Dipteryx Alata Vogel)." In II Brazilian Congress of Development. DEV2021, 2021. http://dx.doi.org/10.51162/brc.dev2021-0102.

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Objetivou-se com esse trabalho estabelecer e desenvolver plântulas de Baruzeiro in vitro. As sementes de Baru foram coletadas em árvores adultas no município de Pires do Rio - GO, desinfestadas em água e detergente, álcool 70% e hipoclorito de sódio 2,5% e lavadas em água destilada autoclavada. Estas foram inoculadas em frascos de vidro contendo meio de cultura MS (Murashige e Skoog, 1962) 0,0 e 50% da concentração dos sais. Experimento 1, foi utilizado diferentes princípios ativos (hipoclorito de sódio e ácido acetilsalicílico - AAS) e diferentes concentrações (Hipoclorito de sódio (0; 0,25; 0,50; 0,75; e 1,00%) e AAS. E o Experimento 2 testando diferentes concentrações do meio MS (MS 0 e MS 50%). Avaliou-se o índice de oxidação; contaminação; à porcentagem de sobrevivência, o comprimento da raiz principal (cm); o comprimento da parte aérea (cm); o número de folhas e o diâmetro do caule (cm). Os experimentos foram instalados em delineamento inteiramente casualizado (DIC). Nas condições em que se realizou este estudo conclui-se que: o estabelecimento in vitro levando em consideração o índice de germinação e sobrevivência para os meios com AAS e NaClO foram de 80,0% e 64% (AAS) e 90,0% (NaClO). Quanto ao uso de MS 0% e MS 50% com carvão ativado, houve percentual médio de 71% de germinação. Para o meio MS 50% e carvão ativado houve percentual médio de 91% de germinação com o T2 com 100% de germinação. ,
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Oliveira, Sara Cristine Farias De, Jessica Bianca Reis De Brito, Pedro Camillo Siqueira Dos Santos, Karine Santos Dos Santos, and Joanne Moraes De Melo Sousa. "INVESTIGAÇÃO DA GERMINAÇÃO E DESENVOLVIMENTO IN VITRO DE EPIDENDRUM CARPOPHORUM BARB. RODR." In II Congresso Brasileiro de Biotecnologia On-line. Revista Multidisciplinar de Educação e Meio Ambiente, 2022. http://dx.doi.org/10.51189/conbiotec/60.

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Introdução: As orquídeas, pertencentes a família Orchidaceae, são muito utilizadas comercialmente para a venda de arranjos ornamentais e, devido a isto, são retiradas excessivamente da natureza, levando muitas delas à extinção. Desta forma, o cultivo in vitro apresenta-se como alternativa para estudos e métodos de conservação para redução de risco de extinção. Objetivo: O objetivo deste trabalho foi avaliar a influência do meio de cultura na germinação in vitro de sementes de E. carpophorum, com o intuito de facilitar o desenvolvimento de um protocolo eficiente de micropropagação dessa orquídea. Material e métodos: O trabalho foi realizado no Laboratório de Biotecnologia da Universidade Federal Rural da Amazônia. A metodologia do trabalho iniciou-se com a coleta de cápsulas imaturas de E. carpophorum no Orquidário Bosque Rodrigues Alves e, posteriormente, foram acondicionadas até o início do processo de assepsia e germinação. Após a assepsia em câmara de fluxo laminar, foram utilizados diferentes meios de cultura, distribuídos nos tratamentos: T1= meio MS (Murashige & Skoog), T2= meio MS + carvão ativado (2,5gL-1), T3= metade dos sais do MS e T4= metade dos sais do MS + carvão ativado. Após inoculação das sementes, os frascos foram mantidos em câmara tipo B.O.D com fotoperíodo de 16h e temperatura de 27ºC. Foram feitas análises qualitativas da germinação, sendo consideradas àquelas sementes que apresentaram protocormos de coloração verde e bom desenvolvimento. Resultados: Os tratamentos com meio MS sem carvão ativado (T1 e T3) apresentaram elevada germinação dos protocormos à medida que foram realizadas as avaliações, o tratamento T1 apresentou melhores resultados em comparação ao T3. Já os tratamentos com meio MS com carvão ativado (T2 e T4) apresentaram rápido entumescimento e melhor desenvolvimento dos protocormos. Ressalta-se que o tratamento T4 apresentou os melhores resultados de germinação e desenvolvimento dentre os demais tratamentos. Estes resultados demonstraram a influência do carvão ativado no desenvolvimento dos protocormos de orquídeas, possivelmente devido a capacidade de maior absorção de nutrientes em meios que contém este tipo de aditivo. Conclusão: Portanto, nota-se que a presença do carvão ativado e o uso de metade do meio de cultura MS pode estimular a germinação e o desenvolvimento in vitro de E. carpophorum.
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