Journal articles on the topic 'Mung bean pulses'

Values for the digestible indispensable amino acid score (DIAAS) of a protein are based on true ileal amino acid (AA) digestibility values obtained in adult humans or in the growing pig as an animal model. An experiment was conducted using growing pigs to determine the true ileal digestibility (TID) values of AA in six cooked Chinese pulses (kidney bean, mung bean, adzuki bean, broad beans, peas and chickpeas). Each pulse was included in a diet as the only source of crude protein (CP). An N-free diet was given to allow determination of gut endogenous AA losses. Seven growing pigs each fitted with a T-cannula at the terminal ileum were allotted to a 7 by 6 incomplete Latin square with seven diets and six 7-d periods. The true digestibility values % for the total indispensable AA were higher (p < 0.001) for broad beans (87.3 ± 2.98) and lower (p < 0.001) for kidney bean (73.3 ± 4.84) than for the other pulses. For the older child (over 3 years), adolescent and adult, the DIAAS (%) was 88 for kidney bean, 86 for mung bean, 76 for chickpeas, 68 for peas, 64 for adzuki bean and 60 for broad beans.

The present study was taken to evaluate the proximate content and dietary fibre compositionof locally available cereal (wheat, maize, oat and barley) and the legumes (mash beans, lentils, mung beansand chickpea). In cereal samples, crude proteins in all cereals were found in the range of 8.75-10.93% butin legumes this range was significantly higher i.e. (19.91-22.06). Crude fibre analysis in cereal samplesshowed values between 1.89-10.6, but in legume samples it ranged between 2.64 to 4.41. Total dietaryfibre was higher in oat and barley 19.0 and 18.34, respectively, whereas total dietary fibre contents inselected legumes ranged between 18.00 (chick pea) to 24.93 (mung bean).

Diversifcation of crop production in the Russian Federation could be partly achieved by the introduction and production of minor and underutilized crops. Green gram or mung bean (Vigna radiata(L.) R. Wilczek) and black gram or urd (V. mungo(L.) Hepper) are grain legume crops cultivated in limited areas in the Russian Federation. Meanwhile, green gram occupies about 8.5 % of the world production area under pulses (without soybean). It is cultivated mainly in countries of Southeast Asia, but production is expanding to the entire subtropical belt of the globe. In our country these crops can be successfully grown on irrigation in a number of regions in the southern area of the European part and the Russian Far East, where the temperatures during their vegetation are about 28–30 °C and always above 15 °C. The purpose of this paper is to summarize the world’s experience in breeding improvement of mung bean and urd as crops with promise for cultivation in certain soil and climatic zones of the Russian Federation. The world production, use of these high-protein crops, history and peculiarities of breeding, including in the USSR, are covered. To expand the production of both crops in the Russian Federation, their popularization and development of breeding are required. Basic requirements for modern varieties include resistance to biotic and abiotic stressors which can be introgressed from wild relatives. The great importance of both crops in the Asian countries led to the rapid development of molecular researches there. The genome of black gram has been fully sequenced, the genome of green gram has been partly sequenced. Some genes and QTL of adaptability traits have been marked and mapped in a number of wild species of the genusVigna. The role of wild relatives in the breeding of crops concerned is discussed. In the world genebanks, signifcant genetic resources of mung bean and urd have been accumulated. All this creates prerequisites for the development of marker-assistant and genomic breeding.

An automatic bio-monitoring system was developed for exploring the life history of insect (Callosobruchus maculates) inhabiting in beans. Callosobruchus maculatus only feeds before exclusion and the demand for food is different in every life stage. The feeding process cracks bean texture fibers and produces feeble ultrasound. Hence the life history of an inhabitant can be explored through spying the feeding cracks. The developed ultrasonic monitoring system consists of a delicate electronic signal-conditioning module, which amplifies, filters, and transforms a biting crack into a square pulse. A computer equipped with software developed in house acquires biting pulses and displays the life history on line. Acquired data are stored for subsequently off-line analysis. The system has been successfully applied to exploration of the life histories of Callosobruchus maculatus inhabiting in Azuki beans and mung beans.

SUMMARYContinuous cultivation of rice–wheat cropping system in the Indo-Gangetic plains is under threat with decline in soil organic carbon (SOC), total factor productivity and overall sustainability. Pulses, an important component of crop diversification, are known to improve soil quality through their unique ability of biological N2 fixation, leaf litter fall and deep root system. Therefore, the effect of inclusion of pulses in the puddled rice system under organic and inorganic amendments on SOC pool and its management indices were evaluated in a long-term experiment after seven cropping cycles. The results indicated that inclusion of pulses in the rice-based system improved the SOC content, being greater in surface soil (0–20 cm) and declining with soil depth. Among the four carbon fractions determined, less labile carbon fraction (Cfrac3) was the dominant fraction in the puddled rice system, particularly under organic treatments, indicating that it is possible to maintain organic carbon for longer time in this system. The rice–wheat–mung bean system resulted in 6% increase in SOC and 85% increase in soil microbial biomass carbon as compared with the conventional rice–wheat system. Application of crop residues, farm yard manure (5 t ha−1) and biofertilisers had greater amount of carbon fractions and carbon management index (CMI) over control and the recommended inorganic (NPKSZnB) treatment in the soil surface, particularly in the system where pulses are included. Interestingly, in the puddled rice system, passive carbon pool is more in surface soil than deeper layers. The relative proportion of active carbon pool in surface layer (0–20 cm) to subsurface layer (20–40 cm) was highest in rice–wheat–rice–chickpea (1.14:1) followed by rice–wheat–mung bean (1.07:1) and lowest in the rice–wheat system (0.69:1). Replacing wheat with chickpea either completely or during alternate year in the conventional rice–wheat system also had positive impact on SOC restoration and CMI. Therefore, inclusion of pulses in the rice-based cropping system and organic nutrient management practices had significant impact on maintaining SOC in an Inceptisol of the Indo-Gangetic plains of India.

Background: Pulses belong to the family leguminosae. Pulses provide protein and fibre, as well as vitamins and minerals, such as iron, zinc, folate, and magnesium. In addition, the phytochemicals, saponins, and tannins found in pulses possess antioxidant and anti-carcinogenic effects, indicating that pulses may have significant anti-cancer effects. It is the practice of germinating seeds to be eaten raw or cooked. Sprouts can be germinated at home or produced industrially. The metabolic activity of resting seeds increases as soon as they are hydrated during soaking. Sprouting grains causes increased activities of hydrolytic enzymes like lipase, improvements in the contents of total proteins, fat, essential amino acids, total sugars, B-group vitamins and starch digestibility, and decrease in phytates and protease inhibitors an increase in amino acid lysine is seen after sprouting, increase in proteolytic activity leads to hydrolysis of prolamins and increased lysine.Methods: Proteins and transaminase activity were estimated from Green mung (Vignaradiata), Cowpea (Vignaunguiculata), Chick pea (Cicerarietinum), Moth bean (Vignaaconitifolia) and Black gram (Vignamungo) in raw (dried) state, after soaking for 12 hours and on sprouting. Students paired t-test was applied to data and statistical significance was established.Results: Chick pea showed highest concentration of proteins whereas Black gram showed the least protein content. Concentration of protein after 12 hours of soaking resulted in chick pea showing highest concentration of proteins whereas green gram showed the least protein content. Chick pea was superior amongst the pulses, because it showed highest protein content after sprouting and highest alanine transaminase activity in 12 hours soaked sample as well as in sprouted sample. Raw moth bean showed highest alanine transaminase activity.Conclusions: Protein content and transaminase activity were found to be highest in sprouted pulses. So, consumption of sprouted pulses should be encouraged. Amongst the pulses studied chickpea (Cicerarietinum) gave the maximum nutritional benefit because of its high protein content and high alanine transaminase activity, as compared to other (green gram, cowpea, moth bean and black gram) pulses.

India is the largest producer of pulses in the world, with 25% share in the global production. The leading pulse producing states are Madhya Pradesh, Maharashtra, Rajasthan, Uttar Pradesh, Karnataka and Andhra Pradesh. The important pulse crops are chickpea (48%), pigeon pea (15%), mung bean (7%), urdbean (7%) and lentil (5%). In the present communication, a survey was conducted from 2015 to 2016 of pigeon pea fields in eighteen locations at Agra, Prayagraj, Aligarh, Basti and Hardoi, districts. The pathogens isolated from pigeon pea plants were Fusarium udum., Sclerotinia rolfsii and Rhizoctonia solani mainly. Physically the textures of soil samples were found clay to sandy at several places among them sandy soil was dominating. Moisture content varied from 6.58 to 11.25%. During the course of study it became evident that pigeon pea (leguminous) plants were found to be wilting. It is also evident that the percent occurrence of wilted pigeon pea plants in different villages of the five districts was in the range of 6.03%-16.01%, whereas, the average wilting occurrence among the district varied from 9.03%-14.5%. Basti district showed maximum percentage of wilt occurrence (14.5%) while Agra, Prayagraj, Aligarh, and Hardoi showed 9.05%, 12.5%, 9.3% and 9.35%, respectively. It was also found that pH of the soil also affected diseases development. As in the fields having pH range of 6.5-8.0, the plants were found to be infected with wilt disease. However, at pH 5.0-6.5 the diseases could not be detected. In Basti, Prayagraj and Aligarh, due to alkaline pH (7.45-7.68) wilting disease on pigeon pea plants occurred frequently. Sandy loam favoured pigeon pea wilt. Sandy loams provide sufficient aeration in the rhizosphere of pigeon pea, which may be required for population build up of inoculum concentration of Fusarium leading to high incidence of wilt.

Mung bean (Vigna radiata L.) is an important pulse consumed all over the world, especially in Asian countries, and has a long history of usage as traditional medicine. It has been known to be an excellent source of protein, dietary fiber, minerals, vitamins, and significant amounts of bioactive compounds, including polyphenols, polysaccharides, and peptides, therefore, becoming a popular functional food in promoting good health. The mung bean has been documented to ameliorate hyperglycemia, hyperlipemia, and hypertension, and prevent cancer and melanogenesis, as well as possess hepatoprotective and immunomodulatory activities. These health benefits derive primarily from the concentration and properties of those active compounds present in the mung bean. Vitexin and isovitexin are identified as the major polyphenols, and peptides containing hydrophobic amino acid residues with small molecular weight show higher bioactivity in the mung bean. Considering the recent surge in interest in the use of grain legumes, we hope this review will provide a blueprint to better utilize the mung bean in food products to improve human nutrition and further encourage advancement in this field.

Ethidium bromide (EB), at 10-5 to 10-4 M, progressively inhibits NAA-induced rooting of mung bean cuttings. Cycloheximide (CH), 6-methylpurine (6-MP) and kinetin (KIN) also inhibited rooting at the same concentrations, although CH and 6-MP were more effective. At 70 and up to 130 hours of incubation, after cuttings received a 1-ml pulse of NAA (10-4 M), they exhibited a progressive increase in the number of observed adventitious roots. The addition of one of the inhibitors, 6-MP, EB or KIN to cuttings, pulsed 48 hours earlier with NAA, showed an initial slight inhibition with increased inhibition over time. CH, however, inhibited rooting immediately after addition. From these and other similar kinetic studies, it appears that 6-MP, EB and KIN operate at the transcriptional level and that CH inhibits translation. Lineweaver-Burk plot analysis of NAA-induced rooting inhibition showed that EB may act as a competitive inhibitor of NAA. Since EB is a known intercalating agent and competitively inhibits NAA-induced rooting, NAA may influence gene expression by ultimately binding to DNA. Studies with space-filling and computer-generated models show that both NAA and EB can bind to certain dinucleotides by an intercalation mechanism.

Infrared (IR) heating is often used for the treatment of liquid and solid foods. IR treatment is known to enhance their shelf life by reducing moisture content and inactivating the microorganisms. Mung bean (a type of pulse from India) is a short season crop; suffers maximum storage loss when compared to other legume grains. The losses are due to moisture and temperature movements. Drying of grains is an important post-harvest operation. IR drying is advantageous over the conventional drying methods. In this paper, the drying of mung bean is considered. An experimental setup is developed to obtain the required moisture and temperature profiles. The equivalent model is simulated using COMSOL multiphysics software and the percentage error between the experimental and simulated models is calculated. Results of numerical implementation are presented and possible further extensions are identified.

The n-hexane extracts of the weeds ‘bhatpata’ Clerodendrum viscosum, ‘kashiature’ Cassia tora, ‘dhakishak’ Dryoptris filix-max, ‘bonmorich’ Croton bonpalandianum and ‘ghagra’ Xanthium strumarium were used to evaluate their effectiveness for suppressing pulse beetle, Callosobruchus chinensis reared on mung bean Vigna radiata grains. The investigations were done with 1, 2 and 4% n-hexane extracts of the weeds and an untreated control. The weed extracts exhibited considerable effectiveness which varied with weed species, concentrations and exposure durations. The higher concentrations showed the higher rate of insect mortality, fecundity, adult emergence inhibition, and grain protection. The LC50 values of the extracts ranged from 5.3 to 7.8, 4.7 to 6.5 and 4.1 to 6.0 g/100 ml at 24, 48 and 72 hours after treatment, respectively. The fecundity inhibition varied from 31.7 to 78.7%, adult emergence inhibition from 33.8 to 81.1%, and grain damage inhibition from 10.3 to 60.1% when ‘bhatpata’ with concentration of 1 g/100 ml and ‘ghagra’ with concentration of 4g/100 ml were applied, respectively. Among the tested weeds, ghagra (4g/100 ml) showed better efficacy against C. chinensis compared to other tested extracts and may be suggested to control pulse beetle and protection of mung bean grains.Bangladesh J. Agril. Res. 41(1): 75-84, March 2016

The objective of this study was the determination of the efficiency of pulsed light (PL) treatments for the decontamination of endive salad and mung bean sprouts, as well as the assessment of quality changes in relation to discoloration and alteration of respiration activity. Produce samples were artificially inoculated with two bacterial test strains Escherichia coli (DSM 498) and Listeria innocua (DSM 20649) and exposed to PL at different energy doses. The inactivation efficiency with regard to the naturally occurring microbiota was also investigated. Besides microbiological investigations, color changes were determined as well as the produce respiration during chilled storage. The results indicated that inactivation of more than 2 log was possible with one flash in the case of fresh-cut salad, while the reduction on mung bean sprouts was limited to approximately 1.6 log with one flash, irrespective if the natural flora or inoculated E. coli or L. innocua were considered. The UV part of the PL proved to be exclusively responsible for the inactivation of microorganisms. Significant lower levels of microbial counts of treated compared with untreated samples were maintained for up to 6 days. In the case of endive salad, a dose-dependent progressive discoloration and increase in respiration was diminished by applying optical bandpass filters, which only slightly affected the inactivation efficiency. In contrast, PL treatments showed a positive effect on color and general appearance of mung bean sprouts, while the respiration was almost unaffected. However, care must be taken with regard to efficiency-limiting matrix effects and impact on food quality. These aspects have to be assessed for each treated product. The integration of PL in industrial food processing plants could be an alternative way to improve the microbial quality of fresh produce, and therefore have a positive impact on public health by reducing the risk of contaminations with pathogenic bacteria.

Heterocyclic compounds are known to activate the defense response or resistance in plants. In order to study their effect as activatorsof systemic acquired resistance (SAR), two series of heterocyclic compounds having azole group were used for the present study i.e.,substituted triazolothiadiazoles and thiazolidine thioureas to obtain new compounds with improved biological activities in mung beans(Vigna radiata (L.)Wilczek, is an important pulse crop of India. These compounds could be used as a solution to chemically mediateddisease control both at the farm level as well as chemical fungicides. For this polyphenol content, flavonoid content, PAL activity,peroxidase activity and protein content were determined in control and after chemical treatment in mung beans var. ‘RMG-344’. Theresults indicated significant changes and a distinct role of polyphenols, flavonoids, PAL, peroxidase and proteins in the defense responseof mung beans after treatment with these chemicals.

The bioactive compound is secondary plant metabolites eliciting pharmacological or toxicological effects in man and animals. Legumes are a valuable source of proteins and nutrients for the majority of the world population. Vigna mungo is one of the important legume crops extensively cultivated in India and other parts of the world. Pulses and legumes have been gaining interest because they are an excellent source of bioactive compounds. The objective of this present review is to compile all relevant information published regarding bioactive components from the Vigna mungo. Various bioactive components reported in Vigna mungo were found and it includes flavonoids, isoflavonoids, phytoestrogens, phenolic acids, enzymes, fibers, starches, trypsin inhibitors, phytic acid, lectins, saponins, tocopherols, fatty acids and proteins. This review clearly demonstrates that Vigna mungo is rich in bioactive components and these components are located in various organs of the plant.

Green gram (Vigna radiata) is considered the chief legume in Pakistan. Thus, current study was conducted to examine the ameliorating effect of phytohormones pre-treatments under salt stress on proteome profile of green gram by sodium-dodecyl-sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The soluble green gram seedlings proteins were resolved on 4% stacking and 12% resolving gels. The SDS-PAGE resolved 24 polypeptide bands ranging from 200 to 17kDa. Among these, 12 out of 24 bands of proteins were essentials house-keeping or growth proteins of green grams. While, 120, 114.6, 51.8, 29.1, and 22.8 kDa bands were over-expressed under 50 to 350mM salt with phytohormones treatments. The others 104.5 kDa, 99.8 kDa, 95.3 kDa, 91.0 kDa, 55 kDa, 46 kDa, and 17kDa bands were related to the GAᴣ, IAA, and SA induced tolerance. Overall 120 kDa, 114.6 kDa, 104.5 kDa, 99.8, 95.3 kDa, 51.8 kDa, 29.1 kDa and 22.8kDa bands were first time identified in the current study. The information retrieved from NCBI protein database, the resolved peptides were principally belonging to 7S and 8S vicilin, 2S, 8S, 11S, and 16.5S globulins. It is determined that seed priming with SA enhanced tolerance in green gram by rapidly synthesizing stress alleviating peptides.Key word: Cluster analysis, dendrogram, mungbean, salt stress, SDS-PAGEINTRODUCTIONVarious world-wide health concerning organization recommended the use of high graded plant protein such as legumes to prevent the risk of metabolic disorder (Hou et al., 2019). Legumes are most important protein crop on the earth. Among the legumes, the green gram is the major pulses. Its seeds are rich in superior quality storage protein, which account 85% of the total protein while, another 15% have not been broadly studied (Yi-Shen et al., 2018). The soluble storage protein comprises of 60% globulins, 25% albumin and 15% prolamins. Globulins are further divided into 3.4% basic-type (7S), 7.6% legumin-type (11S), and 89% vicilin-type (8S) (Mendoza et al., 2001; Itoh et al., 2006). Other than proteins, the green gram seeds also contain starch, fiber, phenolic compound, saponins, vitamins, calcium zinc, potassium, folate, magnesium, manganese and very low in fat that made it meager man’s meat (Hou et al., 2019). It is also a good source of green manure and fodder (El-Kafafi et al., 2015). Its root has ability to fix 30 to 50 Kg/ha atmospheric nitrogen in the soil which is essential for maintaining soil fertility (Chadha, 2010). The green gram is the valuable and the major Rabi pulse crop of Pakistan. Its cultivation area in 2016-2017 was about 179,000 hectares with seed yield of 130,000 tones. In comparison during 2017-2018, it was cultivated on 161,800 hectares land with 118,800 tones seed yield (GOP, 2018). One of the reasons of this 9% decrease in both land and productivity is the shortage of irrigated land due to soil salinity. The salinity induce oxidative bust in the mungbean cells, caused by responsive oxygen species (ROS) such as hydrogen peroxide, singlet oxygen, hydroxyl radical and superoxide radical. The ROS create hindrance in various metabolic processes of plant via interacting with macromolecules like proteins (Alharby et al., 2016). However, phytohormones like gibberellic acid (GAᴣ), indole acetic acid (IAA), and salicylic acid (SA) take part in the biosynthesis of salt tolerance proteins under salinity. These salt tolerance proteins acclimate plants under salinity stress. Application of biotechnology plays a significant role in agriculture (Khan et al., 2017). Therefore, production of particular proteins under salt stress is a specific response of cell which can be analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE is the simple, valid, and cost-effective biochemical marker (Mushtaq et al., 2018). This marker has been widely used to determine the extent of evolutionary variations in crops (El-Kafafi et al., 2015).OBJECTIVES The present study was directed first time with the aim to investigate the toxic effect of sodium chloride (0-350 mM) and stress acclimation by pre-treatment of GAᴣ, IAA, and SA on the proteome profile of NM-92 cultivar of a Pakistani green gram.MATERIALS AND METHODSThe present study was replicated thrice in the plant laboratory of Department of Genetics, Faculty of Science, and University of Karachi. The seeds of mung bean cultivar NM-92 were acquired from National Agricultural Research Centre (NARC), Islamabad. These freshly collected 15 seedsˉ1 treatment / replication were divided into two sets. The first was named as sodium chloride (SC) stress treatments were imbibed in sterile distilled water (DW) whereas, second set soaked in gibberellic acid (GAᴣ) (BDH Chemicals, England), indole acetic acid (IAA) (Fluka, Switzerland), and salicylic acid (SA) (J.T. Baker, Holland) in the separate beaker for 24 hours under dark condition. After 24 hours, given ample time to both the sets at room temperature. After recovery, all 20 treatments were sown in the 150 X 30 mm sized petri-dishes containing 0, 50, 150, 250, and 350 millimolar (mM) sodium chloride solution (Fisher Scientific, UK) for 72 hours.Protein extraction: Protein extraction was done by taking 0.3g of seedlings in an ice chilled mortar and crushed by adding 600µL 0.2 M Tris-HCl buffer having pH 7.5 contained 5% SDS (w/v) and 5% 2-mercaptoethanol (v/v). The homogenate was incubated at 0oC for 30 min., boiled in the water bath for 3 min. at 100oC. Samples were centrifuged in Heraeus Biofuge D-37520, Germany for 30 min. at 8000 rpm. The protein supernatant was saved at below 0°C for quantitative and qualitative determination with minor modifications. The total soluble protein content of the samples was estimated via “Bovine Serum Albumin (BSA) standard curve” and explicit in µg protein milligramˉ1 fresh weight of mung seedlings.Bovine serum albumin standard curve (2000 μg/mL): Total protein standard curve was made by dissolving 0.05g of Bovine Serum Albumin (BSA) in 25mL of distilled water. Ten serial dilutions were made from 0.1 mL to 1mL by BSA solution then performed Lowry. A standard curve of total proteins was plotted by taking BSA absorbance at Y-axis and 2000 μg BSA / mL at X-axisSample preparation for SDS-PAGE: For qualitative assessment of total proteins; the 35μL of saved protein supernatant was combined with 15μL of sample diluting buffer (SDB). The SDB was made up of 0.0625 M Tris-HCl pH 6.8 with 2% of SDS, 10% of glycerol, 0.003% of bromophenol blue dye and 5% of 2-mercaptoethanol. Boil the 50μL protein SDB supernatant at 100oC in water bath for 3 min., centrifuged at 6000 rpm for 4 min. The supernatant was loaded on SDS-PAGE gel with the given formulae. The SDS- PAGE: Total proteins were fractionated via SDS-PAGE with 4% stacking and 12% resolving gel. The resolving gel of 12% was made by taking 6mL solution A, 1.8 mL 3 M Tris 1 M HCl buffer pH 8.8, 144μL 10% SDS, 5.74 mL sterile distilled water, 720μL 1.5% ammonium persulphate (APS) in deionized water and 10μL TEMED. While, stacking was composed of 1.25mL of solution A, 2.5mL of 0.5M Tris 1M HCl buffer pH 6.8, 100μL 10% SDS, 1.8 mL of distilled water, 500μL 1.5% APS and 12μL TEMED. Solution A was prepared by conjoining 30% acrylamide and 0.8% N, N’-methylene-bisacrylamide in deionized water. To avoid polymerization in the beaker; the prepared solution was quickly poured into the 3 mm thick gel plates after adding TEMED. The stacking was lined over resolving gel, then combs were inserted between the gel plates of SCIE-PLAS TV-100 separation system, UK, and allowed to polymerize for ½ an hour. After polymerization gel was placed in the tank which were filled with Tris-Glycine buffer (electrode buffer) pH 8.4 then combs were removed. The electrode buffer contained 0.3% Tris, 1.41% Glycine and 0.1% SDS in 2000mL d/w. The gel was pre-run for 15 min. at 60 volts and 120 mA currents. The prepared SDS-PAGE samples were loaded in wells with BlueStepTM Broad Range Protein Marker, AMRESCO, USA as standard and run at 60 volts & 120 mA for about 45 min. When samples entered in resolving gel, and then gave 100 volts and 200 mA currents for around 2.5 hours. Furthermore, electrophoresis was carried out at a constant watt.The Gel was washed with 30% ethanol on Uni Thermo Shaker NTS-1300 EYELA, Japan at the constant shaking for 30 min. Then gels were placed in 10% glacial acetic acid in 50% methanol solution (Fixative) for 24 hours. SDS Gel was stained until protein bands were visible thereat placed as 5% of Methanol in 7.5% acetic acid glacial solution to destain the bands background. SDS-PAGE stain composed of 0.125% coomassie brilliant blue R-250 dissolved in 40% of Methanol and 7% acetic acid glacial solution. The stain was stirred on Magnetic stirrer & hot plate M6/1, Germany for 6-10 hours before used. Photographs were taken by Sanyo digital camera VPC-T1284BL and bands were scored through numbering pattern. Gels preserved in 10% acetic acid solution at 4°C.Interpretation of bands and data analysis: The total soluble protein bands relative mobility calculated by below formulae and Dendrogram was constructed via SPSS v. 20Where,F=(Migrated distance of protein band)/(Migrated distance of dye front)Slop=(Log MW of protein marker lower limit band–log〖MW of protein marker upper limit band )/(RF protein marker lower limit band –RF of protein marker upper limit band)RESULTS:The total soluble proteins extracted from green gram were perceived by SDS-PAGE Blue StepTm broad range biochemical markers. The protein-based marker was used to evaluate the toxic effect of sodium chloride along with pre-treatments of GAᴣ, IAA, and SA on proteome assay. In the current work, seedlings total soluble proteome resolved 24 polypeptide bands ranging from 200 to 17.1 kDa were recognized by using SDS-PAGE. The figure 1 showed Dendrogram assay, which classified the 20 treatments of SC, GAᴣ, IAA and SA into two major clusters where, the cluster I was the largest one (figure 1). Cluster I consisted of 15 treatments that further divided into I-A, and I-B. The pre-treatments of SC50+SA, SC150+SA, SC250+SA, and SC350+IAA were grouped together into C-1 of sub-cluster I-A. The C-2 of sub-cluster I-A, pre-treatment SC350+SA was most diverse among 20 treatments. The C-1 treatments showed 99% homology when compared with each other while, it was 97% similar with C-2. The sub-cluster I-B comprised another 10 treatments, SC0+GAᴣ, SC50+GAᴣ, SC150+GAᴣ, SC250+GAᴣ, SC350+GAᴣ, SC0+IAA, SC50+IAA, SC150+IAA, SC250+IAA, and SC0+SA that were also 99% similar for total proteins. Sub-cluster I-B pre-treatments was exhibiting 94% homology with the sub-cluster I-A. The second cluster was the smallest one that was divided into two sub-clusters, II-A and II-B. The II-A was comprised of SC50, SC150, and SC250 while, sub-cluster II-B consisted of SC0 and SC350. Within each sub-cluster, pre-treatments expressed 99% homology whereas, II-A was 97 different from II-B. Furthermore, cluster I showed 75% similarities with cluster II (figure 1). The seedlings storage proteome profile of green gram was shown in table 1.The results showed that 120kDa, 114.6 kDa, 51.8 kDa, 29.1 kDa and 22.8 kDa proteins bands were not induced at 0 mM SC, GAᴣ, IAA, and SA. The table 1 depicted the presence of 120 kDa and 114.6 kDa bands only at 350 mM SC level with all phytohormones treatments. Similarly, 51.8 kDa protein bands were appearing at 150SC, 250SC and 350SC stress with phytohormones. Based on the information collected from the NCBI protein database, this peptide was related to the 8S globulin alpha subunits. The two other, 7S globulins sub-units having 29.1kDa and 22.8 kDa molecular weights bands were synthesized under 50mM, 150mM, 250mM, 350mM SC stress with phytohormones. Concerning protein polypeptide of molecular weight 104.5 kDa, 99.8 kDa, 91.0 kDa, 55.0 kDa, and 46.0 kDa, those were induced by GAᴣ, IAA and SA at 0 to 350 mM SC. While, 17kDa protein band was appearing in SA, and IAA treated samples and 95.3kDa band was only present in SA treatment. Other 12 protein bands were present in all treatments proved as house-keeping proteins of green gram (table 1).DISCUSSIONThe SDS-PAGE profiling for proteome is the reliable and applied biochemical approach that has been used as biochemical marker in various crop differentiation, and characterization. In the current study, first time SDS-PAGE was utilized to investigate the impact of GAᴣ, IAA, and SA pre-soaking on green gram under salt toxicity. The salt toxicity adversely affects all seed, seedling, and plant metabolic process (Parveen et al., 2016). At salt toxicity, the endogenous GAᴣ, IAA, and SA levels markedly decrease (El-Khallal et al., 2009). In such condition, exogenous application of GAᴣ, IAA, and SA enhance seedlings survival rate by increasing synthesis of seed storage proteins. Likewise, our Dendrogram characterization based on 20 treatments showed significant diversity under 0 to 350 mM SC stress. The salicylic acid treatments were grouped together except SC0+SA treatment, exhibiting a close relationship, which proved its acclimating role under salt stress. These findings will help plant breeder toward enhancing food quality and quantity of green gram in future breeding programme on saline sodic land.The SDS-PAGE assay revealed 200. kDa, 109.4 kDa, 77 kDa, 68 kDa, 49 kDa, 38 kDa, 33 kDa, 26 kDa, 24 kDa, 22 kDa, 21 kDa and 19 kDa fractions as essential green gram proteins. Among these, 68 kDa, 49 kDa, 33 kDa, 26 kDa, 24 kDa and 21 kDa peptides were seed biotinylated isoform protein (Riascos et al., 2009), putative NADH-ubiquinone oxidoreductase subunit H (Gostinčar et al., 2019), heat shock protein 33 (Hamidian et al., 2015), globulin protein, seed coat / maturation protein (Dhaubhadel et al., 2005), and protein for dimerization. While, 22 kDa proteins belonged to the class of prolamin alpha zein Z1C1_2, Z1C1_4, and Z1C1_8 precursors, and 19kDa peptide was related with Z1A1_2, Z1A2_2, and Z1B_6 precursors (Miclaus et al., 2011). Further, the 91 kDa peptide is sucrose synthase SS1 protein, and 77kDa protein is the NADPH-cytochrome P450 reductase (Wang et al., 2004). Also, the phosphatase-associated two other proteins having 46 and 55 kDa molecular weight were reported earlier in Mucuna pruriens. Hameed et al. (2012) and Malviya et al. (2008) found 55 and 46kDa peptides as 7S vicilin small sub-units and 17kDa as 11S globulins sub-unit in the studied Vigna radiata. Some other molecular weight proteome such as 68 kDa and 49kDa are 7S vicilin, 33kDa is 8S vicilin, 38 and 26kDa 8S globulins, 24kDa 11S globulins, and 22kDa 16.5S globulins. These proteins required for germination and seed establishment of green gram plant (Hameed et al., 2012).The vast accumulation of 23kDa and 22kDa peptides under salt stress by salicylic acid, were reported previously in the mangrove Bruguiera parviffora and Zea mays (El-Khallal et al., 2009). Correspondingly, El-Kafafi et al. (2015) reported the presence of 115kDa, 23kDa, and 22kDa bands in the salt tolerant lines of green gram. These proteomes induced under salt stress may play a pivotal part in the stress acclimation and osmotic adjustment. Similarly, the induction of 104 kDa and 100kDa MW polypeptide by SC stress in the salt tolerant genotypes of green gram indicated the functional role of phytohormones in various metabolic and defense response El-Kafafi et al. (2015); Alharby et al. (2016), El-Khallal et al. (2009), Qados (2010). Ali et al. (2007), Alharby et al. (2016), and El-Kafafi et al. (2015) observed 17kDa, 26kDa, 33kDa and 77kDa bands involving in salt tolerance and can be considered as a positive biochemical marker for salt stress. Further, 26 kDa MW peptide also functions as osmotin under the salt stress that involved in enhancing the accumulation of glycine betaine and proline in the cells. Hence, proteome assay of green gram showed that GAᴣ, IAA, and SA could regulate the expression of salt stress proteins that are anticipated to play a crucial part in the salt tolerance mechanism. Likewise, the involvement of phytohormones in the induction of changes in the proteome profile pattern was attributed to their part in managing cell division by regulating some genes of apical meristems.CONCLUSIONFinally, the results revealed the presence of the ten new bands with MW of 200kDa, 120 kDa, 114.6 kDa, 109.4kDa, 104.5kDa, 99.8kDa, 95.3kDa, 51.8kDa, 29.1kDa and 22.8kDa have not reported previously under salt stress with phytohormones treatments in green gram. Furthermore, it was observed that phytohormones alleviate the negative impact of salt stress on green gram by enhancing synthesis of salt defense polypeptides. Hence, higher accumulation of proteins was observed in salicylic acid treated seedlings. Thus, present work recommended the pre-soaking of phytohormones to overcome the toxic impact of sodium chloride on green gram. Further research is needed on a biomolecular level to reveal the mechanism of signalling pathways under sever salt stress.CONFLICT OF INTERESTBoth authors have declared that no disagreement of interest regarding this research.REFERENCES Alharby, H. F., E. M. Metwali, M. P. Fuller and A. Y. Aldhebiani, 2016. The alteration of mRNA expression of sod and gpx genes, and proteins in tomato (Lycopersicon esculentum Mill) under stress of Nacl and/or ZnO nanoparticles. Saudi journal of biological sciences, 23(6): 773-781.Ali, A., M. Mageed, I. Ahmed and S. Mariey, 2007. Genetic and molecular studies on barley salt tolerance. In: African crop science conference proceedings. pp: 669-682.Chadha, M., 2010. Short duration mungbean: A new success in South Asia. Asia-Pacific association of agricultural research institutions.Dhaubhadel, S., K. Kuflu, M. C. Romero and M. Gijzen, 2005. A soybean seed protein with carboxylate-binding activity. Journal of experimental botany, 56(419): 2335-2344.El-Kafafi, E.-S. H., A. G. Helal, S. F. El Hafnawy and R. Flaah, 2015. Characterization and evaluation of some mungbean genotypes for salt tolerance. World applied science journal, 33(3): 360-370.El-Khallal, S. M., T. A. Hathout, A. Ahsour and A.-A. A. Kerrit, 2009. Brassinolide and salicylic acid induced antioxidant enzymes, hormonal balance and protein profile of maize plants grown under salt stress. Research journal of agriculture biological sciences, 5(4): 391-402.GOP, 2018. Pakistan economic survey from 2017 to 2018. Ministry of Finance. Islamabad. Government of Pakistan. Accessed 18-8-2019, http://www.finance.gov.pk/su rvey/chapters18/02-Agriculture.pdf.Gostinčar, C., M. Turk, J. Zajc and N. Gunde‐Cimerman, 2019. Fifty aureobasidium pullulans genomes reveal a recombining polyextremotolerant generalist. Environmental microbiology, 21(10): 3638-3652.Hameed, A., M. Qureshi, M. Nawaz and N. Iqbal, 2012. Comparative seed storage protein profiling of mung bean genotypes. Pakistan jouranl of botany, 44(6): 1993-1999.Hamidian, M., J. Hawkey, K. E. Holt and R. M. Hall, 2015. Genome sequence of Acinetobacter baumannii strain d36, an antibiotic-resistant isolate from lineage 2 of global clone 1. Genome announced, 3(6): e01478-01415.Hou, D., L. Yousaf, Y. Xue, J. Hu, J. Wu, X. Hu, N. Feng and Q. Shen, 2019. Mung bean (vigna radiata l.): Bioactive polyphenols, polysaccharides, peptides, and health benefits. Nutrients, 11(6): 1238.Itoh, T., R. N. Garcia, M. Adachi, Y. Maruyama, E. M. Tecson-Mendoza, B. Mikami and S. J. A. C. S. D. B. C. Utsumi, 2006. Structure of 8sα globulin, the major seed storage protein of mung bean. Acta crystallographica section D: Biological crystallography, 62(7): 824-832.Khan, F. F., K. Ahmad, A. Ahmed and S. Haider, 2017. Applications of biotechnology in agriculture-review article. World journal of biology biotechnology, 2(1): 139-142.Malviya, N., S. Nayak and D. Yadav, 2008. Characterization of total salt soluble seed storage proteins of grain legumes using sds-page. Bulletin de ressources phytogénétiques(156): 50.Mendoza, E. M. T., M. Adachi, A. E. N. Bernardo and S. Utsumi, 2001. Mungbean [Vigna radiata (L.) wilczek] globulins: Purification and characterization. Journal of agricultural food chemistry, 49(3): 1552-1558.Miclaus, M., J.-H. Xu and J. Messing, 2011. Differential gene expression and epiregulation of alpha zein gene copies in maize haplotypes. PLoS genetics, 7(6).Mushtaq, F., S. A. Jatoi, S. S. Aamir and S. U. Siddiqui, 2018. Genetic variability for morphological attributes and seed protein profiling in chili (Capsicum annuum L.). Pakistan jouranl of botany, 50(4): 1661-1668.Parveen, A.-u.-H. M., J. Akhtar and S. M. Basra, 2016. Interactive effect of salinity and potassium on growth, biochemical parameters, protein and oil quality of soybean genotypes. Pakistan journal of agricultural sciences, 53(01): 69-78.Qados, A., 2010. Effect of arginine on growth, nutrient composition, yield and nutritional value of mung bean plants grown under salinity stress. Nature, 8: 30-42.Riascos, J., W. Burks, L. Pons, A. Weissinger and S. Weissinger, 2009. Identification of a soybean seed biotinylated protein as a novel allergen. Journal of allergy cinical Immunology, 123(2): S24.Wang, S. Y., J. H. Wu, T. Ng, X. Y. Ye and P. F. Rao, 2004. A non-specific lipid transfer protein with antifungal and antibacterial activities from the mung bean. Peptides, 25(8): 1235-1242.Yi-Shen, Z., S. Shuai and R. FitzGerald, 2018. Mung bean proteins and peptides: Nutritional, functional and bioactive properties. Food nutrition research, 62.

Māsh (Vigna mungo (L.) Hepper belongs to the family Papilionaceae. It is one of the important legume crops extensively cultivated in India and other parts of the world. Pulses and legumes have been gaining interest because they are an excellent source of bioactive compounds. The objective of this present review is to compile all relevant information regarding the medicinal uses of Vigna mungo. It is rich in flavonoids, isoflavonoids, phytoestrogens, phenolic acids, enzymes, fibers, starches, trypsin inhibitors, phytic acid, lectins, saponins, tocopherols, fatty acids, and proteins. Most of the reported components are from the seed part of the black gram. Various processes like cooking, soaking, and germination affect bioactive components. Studies have shown the presence of bioactive compounds in other parts of the plant like leaves, pods, roots, stems, etc. which are normally considered as a waste product. Hence there is a need to isolate and characterize novel bioactive components from other parts of the black gram plant. This review demonstrates that Vigna mungo is rich in bioactive components and able to cure and prevent diseases in addition to its basic nutritional value. Keywords: Māsh, Vigna mungo, black gram, bioactive components, legumes

Grain yield of blackgram or urdbean, a popular and highly proteinaceous pulse crop of India, is very low. This is possibly because of its cultivation on marginal and submarginal lands of low soil fertility where little attention is paid to adequate fertilizer, especially phosphorus and nitrogen. Although large achievements have undoubtedly been made in plant nutrition in cereals like wheat and rice, much less attention has been devoted to the development of suitable agro-techniques for production of pulses, especially blackgram. Inoculation with efficient Rhizobium strains and fertility management are important in the production technology of this crop. Though blackgram is regarded as excellent in symbiotic nitrogen fixation, many workers (Pate & Dart, 1961; Shanthakumari & Sinha, 1974) have opined that actual nitrogen fixation starts about 3 weeks after sowing and the senescence of nodules after profuse flowering. This results in nitrogen hunger at initial stages of crop growth as well as in the reproductive stage. Beneficial effects of starter nitrogen in blackgram have been reported by some workers (Singh et al. 1975). However, symbiotic nitrogen fixation may not be adequate to satisfy the N need of developing pods. Since no information is available on the effect of top dressing of nitrogen in the reproductive phase, the present investigations were planned to study the effect of Rhizobium. inoculation and phosphorus as well as time and method of N application on growth and yield of blackgram and its residual effect on fodder sorghum.

Purpose – The purpose of this paper is a study designed to utilize Vigna mungo L. (black gram) flour to improve the protein quality of wheat through supplementation. Wheat is utilized as a cereal crop all over the world, but its protein quality is inferior owing to the deficiency of amino acids like lysine. Design/methodology/approach – Black gram seeds were roasted and germinated and then incorporated at 10, 15 and 20 per cent level in wheat flour. The composite flour was evaluated for its chemical composition, physiochemical properties and rheological characteristics, and cookies were developed from the composite flour. Findings – Chemical composition of composite flour revealed significant results (p < 0.05). The level of crude protein increased from 9.69 to 11.79 per cent, while ash and crude fat content enhanced from 0.33 to 1.80 per cent and 1.13 to 2.40 per cent, respectively. There was a significant effect (p < 0.05) on the sedimentation value by the addition of black gram in wheat flour. Water absorption of composite flour was higher than control (52.21 per cent), as maximum value was observed in flour having 15 per cent germinated black gram flour (69.45 per cent). Dough development time also increased from 2.90 min to 4.80 min. The pasting properties revealed significant results for all the parameters. Cookies were developed from composite flour, and sensory evaluation has shown that addition of pulse flour at 15 per cent yields cookies with better hedonic response. Originality/value – The black gram is a locally grown legume crop, but there is a lack of systematic approach for its chemical composition and product development. Scientists are in urge to explore such economical and assessable food ingredients to cope with the nutritional deficiencies prevailing in the developing societies. In this regard, black gram has been recognized as a rich source of nutrients, so it can be exploited to improve wheat protein quality.

Pigeon pea is an important pulse crop grown in diversified cropping systems in India. In the rainy season of August 2011 and September 2012, pigeon pea cv. LRG 30 plants with leaf necrosis having wrinkled margin on one side were observed in Kadiri mandal of the Anantapuram district of Andhra Pradesh (A.P.), India. Symptoms included necrotic spots on young leaves followed by wilting of leaves, petiole and branch/axillary shoot proliferation, with small leaves having mosaic symptoms. Symptomatic leaves were sap-inoculated onto 10 seedlings of cowpea (cv. Pusa Komal) using 0.01 M phosphate buffer (pH 7.0). Localized necrotic lesions developed in all the inoculated plants after 2 days post inoculation. Field symptoms were reproduced on healthy pigeon pea upon back inoculation using single lesions of infected cowpea leaves. In direct antigen coating (DAC)-ELISA, all the infected pigeon pea and cowpea leaf samples were positive to a polyclonal antiserum specific to Tobacco streak virus (TSV) supplied by ICRISAT, India. Total RNA was extracted using infected pigeon pea and healthy leaf samples by TRI Reagent (Sigma). Reverse transcription (RT)-PCR was carried out using primers specific to the coat protein (CP) gene of TSV (1). A product of the 700-bp DNA fragment was obtained in field-infected pigeon pea samples but not in healthy controls. The amplicon was cloned into PTZ57R/T using the Ins TA clone PCR kit (Fermentas). Recombinant clone was sequenced in both directions and the CP gene sequence obtained was deposited in GenBank (KF220492). Sequence analysis of the CP gene of TSV from pigeon pea shared 98 to 100% identity with Indian TSV isolates originating from different hosts including groundnut (FJ355948), mung bean (FJ749259), and sunflower (DQ864448), and 88 to 92% similarity with TSV type isolate (white clover: X00435) both at nucleotide and amino acid levels. TSV belongs to the genus Ilarvirus of family Bromoviride and has a wide host range. TSV is pollen borne, assisted by thrips causing mechanical injury (2). To our knowledge, this is the first report of TSV on pigeon pea in India and was widespread in Anantapuram, Kadapa, Kurnool, and Mahbubnagar districts of A.P. Yield loss depends on the stage of infection as early infection resulted in complete failure of the crop. TSV was prevalent on many legume crops such as black gram, green gram, and groundnut in A.P, Tamil Nadu, Karnataka, and Maharashtra states (3). TSV infection of pigeon pea may pose a serious implication for pulse production. References: (1). A. I. Bhat et al. Arch. Virol. 147:651, 2002. (2). M. Sharman et al. Australian Plant Dis. 6:54, 2011. (3). K. Vemana and R. K. Jain. Indian J. Virol. 21:117, 2010.

Background: Pulses are leguminous crops which not only increase the soil fertility, its fitness and maintain soil health but also essential to meet the nutritional demand of burgeoning human population particularly in developing countries. Mungbean is a major pulse crop of Zone IIa (Transitional Plain of Inland Drainage Zone) of Rajasthan state. Farm profitability can also be enhanced by augmenting farm productivity. Selection of high yielding genotypes may play a vital role to achieve sustainable high agricultural yield at farmer’s field. Therefore, it is prerequisite to identify the suitable genotypes for this zone since the available varieties were not tested for its adaptability. The current study was aimed to evaluate twelve mung bean genotypes for seed yield with four checks in Zone IIa of Rajasthan.Methods: The mung bean crop was raised during Kharif 2018 and 2019 at Agricultural Research Station, Fatehpur- Shekhawati, Sikar (Rajasthan). Seed yield and its ancillary characters have been observed by following standard protocols. Present experiment was conducted in randomized block design with three replications. The material was sown in a four row plot of 4 m length with a spacing of 30 cm between rows and 10 cm between plants.Result: The performance of genotypes RMG 1098, RMG 1132, RMG 1134, RMG 1139 and RMG 1147 were superior to the zonal, state checks and other tested genotypes. The selected high yielding mung bean genotypes can increase farm output per se and farm profitability by sustaining soil health, fitness and productivity of this region.

Legume crops are very important group of plants that belong to Fabaceae or leguminaceae family and are rich in different essential amino acids. These legumes prove to be the cheapest and most easily accessible source of required proteins. Humans need proper amount of proteins and essential amino acids that are crucial for the proper functioning of the body. In developed countries a large number of diverse diets including meat, fish, protein, drinks and food supplements are readily available but the developing countries having very small income per capita cannot afford all of these commodities. They fulfill their nutritional value by using legume crops. But in developing countries pulses face a number of biotic challenges. They have low production due to less improved varieties. Major damage to the pulse crops is due to the range of bacterial, fungal and viral diseases that drastically reduce the crop production per unit area. Major diseases of pulse crops include chickpea (Blight, Fusarium wilt, root rot), Green and Black gram (Leaf crinckle virus, Mung bean yellow mosaic virus, Mungbean phyllody disease, Cercospora leaf spot, Powdery Mildew), lentil (Ascochyta Blight, Stemphylium Blight, Anthracnose, Botrytis grey mold, Lentil Rust) and soya bean (Charcoal rot, White mold, Louisiana, Root rot, Septoria glycines, Sclerotium rolfsii, Black root rot). These diseases can be controlled by many physical as well as biological methods that have low cost of production and safe to environment. The detailed elaboration of major pulse diseases and their effective control methods have been summarized in this review paper.

Global demand for pulses such as the mung bean has grown in the last years. For successful production of these crops it is necessary to use high quality seeds. Methodologies based on X-ray image analysis have been used as a complementary tool to evaluate the physical quality of seeds due to their speed and potential for automation. The aim of this study was to evaluate the efficiency of X-ray analysis for non-destructive evaluation of the physical quality of Vigna radiata seeds and to relate the variables obtained with their physiological potential. For this, seeds from eight lots were X-rayed and subsequently subject to germination test. In total, 18 physical and physiological parameters were determined. The X-ray image analysis was efficient for evaluating the internal morphology of Vigna radiata seeds and allowed the identification of various damage types. However, it was not possible to relate the physical variables to the seed quality as the lots presented similar germination percentage. Physical variables such as solidity and circularity are related to percentage of root protrusion and length of seedling hypocotyl. Low relative densities indicate deteriorated tissues, related to severe morphological damage and non-viable seeds.

Background: The main pulses grown in India are chickpea, arhar, lentil, black gram, mung bean, moth bean, horse gram, pea, khesari, cowpea, etc. Black gram is fourth major pulse crop in India, that contributes 13 and 10 per cent of total area and production respectively. This is annual plant that attains 30-100 cm height and its stem is covered with brown hairs and much branched from the base. The pods are long and cylindrical being 5-6 cm length and 4-10 seed in pods. The seeds are generally black, very dark brown. Methods: The field experiment was conducted in kharif-2019 at research farm of Tirhut College of Agriculture Dholi, to study the effect of integrated nutrient management on growth, yield parameters and the yield of black gram cv-PU-31, by the use different sources of nutrient in a integrated manner such as three level of fertilizer i.e. F1-75, F2-100 and F3-125% RDF and two levels of organic manure i.e. M1-control and M2-FYM @ 5 t ha-1 and three levels of biofertilizer i.e., B1-rhizobium, B2-nutrient mobilizer, B3-rhizobium+ nutrient mobilizer. The treatments were allocated in randomized block design (factorial) and replicated thrice. Result: The results revealed that F3 produced taller plants, more dry matter, crop growth rate (CGR) yield attributes resulting higher yield of grain and straw (10.78, 22.61 q ha-1 respectively) which was statistically at par with plant height, dry matter, crop growth rate, yield attributes and yield of grain, straw and highest harvest index (10.73, 22.20 q ha-1 and 32.58% respectively) to F2. Among addition of organic manure significantly maximum plant height, dry matter and crop growth rate and yield attributes resulting maximum yield of grain, straw and harvest index (11.2, 22.79 q ha-1 and 33.31% respectively) was found in M2 over M1. In biofertilizer treatments, B3 recorded higher plant height, dry matter and crop growth rate, yield attributes resulting in significantly higher yield of grain, straw and harvest index (10.26, 21.90 q ha-1 and 31.92% respectively) over B1 and B2.

Mung bean known locally as “Masho” is a recent introduction in the Ethiopian pulse production and grown in few areas of the country and constrained by different biotic factors.However, the relative importance of each pest across locations has not been assessed and well profiled to sound management strategy. The objective of this study was to assess distribution and intensity of major pests on mungbean.The survey was made in 2016 and 2017 main cropping seasons following the main roads and accessible routes in each survey district (lowland areas of North Shewa Zone) and stops were made at every 5 -10 km intervals based on vehicles odometers as per mung bean fields available. Five stops were made in each mung bean field by moving “X” fashion at each stop interval using quadrants and data were collected from each. The result indicated that, Apion clavipes was most prevalent insect pest of mung bean in surveyed areas with maximum prevalence of 100%. Similarly, leaf blight and leaf spot were most prevalent diseases of mung bean in all districts. On the other hand, survey revealed that Cyperus spp. was frequently observed weed in both seasons with the highest weed density per m2. In 2017, weed Spps. Cyperus rotundus and Echinocloacolona L. were more frequently obtained weeds of mung bean in the surveyed districts with the consequent frequencies (77.42, 70.97%) and densities (16.11, 2.8 per m2). The current study indicated that a complex of pests exist on mung bean in the studied areas. Therefore, pest management strategies need to be devised.

Pulses are popularly known as poor man’s meat. Pulses mainly constituted of gram, arhar, mung, urad and lentil etc. The energy content of most pulses have been found to be between 300 and 540 Kcal/100g. India is the largest producer and consumer of pulses in the world contributing around 14.36% of the total global production. Decision support system (DSS) has been designed and developed using a visual basic platform for major pulse crops grown in India. The main objective is to provide decision support regarding recommended water, weed, pest and diseases management, state wise varieties and nutrient management based on the soil test value to the farmer, agriculture workers, students, research workers, extension workers and others, even in absence of the experts. The system is console application, menu driven and user friendly. A sincere effort has been made with the available data to develop the decision support system to bridge the gap between the farmers and the experts using visual basic platform.

Pulse crops are important components of crop production in Ethiopia's smallholders’ agriculture, providing an economic advantage to small farm holdings as an alternative source of protein, cash income, and food security. Mung bean is one of the most important cash crops in the low land of the North Shewa zone. This research was, therefore, initiated to provide an insight of scaling up of Rasa (N-26) mung bean variety and documenting best approaches to maximize production and productivity of the crop through improving the availability of improved varieties to farmers. The activities were done in the low lands of North Shewa areas of Efiratana gidim, Kewot, Shewarobit, Ensaro, and Merhabetie districts, under main growing season for two consecutive years (2016 – 2017) using Rasa variety. About 54 ha of land were covered through disseminating 2.17tons seed and a total of 203 (including 2 female farmers) farmers participated and directly benefited during the dissemination of mung bean variety. The productivity of mung bean in the area was boosted to 1.6 tons ha-1 and a yield advantage of 45.5% was obtained from improved varieties compared to the local cultivar. The introduction of the improved variety Rasa (N-26) which has a large seed size, high biomass, and grain yield attracts the attention of farmers, experts, and local traders. Developing and verifying pesticides, technical backstopping and supplying of early generation seed, and also introducing post-harvest storage materials is very crucial for sustained production and productivity improvement of the crop.

Background: This article discloses information related to a recent patent filed by the author on Extracts of freshly cut farm birds and animals. Objective: The objective was to produce “Liquid-Protein” extracts obtained from live cells of protein rich meat of farmbirds or animals as well as from baby plants of pulses. Method: Freshly cut meat pieces or sprouts of pulses are put in water and pulse-heated for 30 minutes. The nutritious water extract of these is taken that contains amino-acids/proteins and some signaling chemicals emitted from the stressed live cells. Results: The heat-stressed animal cells (of Chicken) released Creatine and many other nutrients in the extract along with Guanosine triphosphate/Guanosine diphosphate/ Guanosine monophosphate/ Inosine Mono Phosphate (GTP/GDP/ GMP/IMP) showing a UV absorption peak at 249 nm. This paper analyses the UV -Visual Absorption spectra and proton NMR data for the extracts. It is disclosed that the vegetarian baby plant cells of pulse seeds released (ATP) Adenosine Tri Phosphate (264 nm peak) along with Resveratrol (306 nm peak) but did not produce Creatine and such an extract had side effects. Conclusion: Cells of birds/animals are similar to those for humans, and the signaling chemicals in the non-vegetarian extract are non-toxic and 100% compatible to humans as compared to plant cell extracts with incompatible chemicals. Since meat-cells manage to “live” for longer than 10 hours with out blood/oxygen supply, by an unclear anaerobic cell respiration involving Creatine and GTP/GDP/GMP/IMP, the extract from these meat cells holds the key for metabolic cell repair for anti-aging of humans.

A large proportion of the Indian population is vegetarian and pulses are important sources of protein in the daily diet .In this paper an attempt has been made to summarize the overall nature of area, production and productivity of mung in India. By and large there has been considerable expansion in area, production and productivity of mung in all the states under study including whole India during the study period. Among the states under study, the maximum annual growth in area (9.75%) and production (14.55%) of mung was observed in Rajasthan. Bihar stands first in productivity of mung among the states under study. Rajasthan, Karnataka and Andhra Pradesh have fails to reach national average per hectare production of 367.37 kg/ha. In this paper an attempt has been made to summarize these measures along with some new measures with an objective to study the yield sustainability of particular crop over the growing regions and compare across the states/regions. Sustainability in yield of mung in different states along with whole India has been measured with the help of existing and proposed measures of sustainability indices. Whole India is showing higher sustainability in yield of mung as per the two existing and proposed methods. According to all the indices including developed two methods Rajasthan is having comparatively lower sustainability to produce mung among the states under study. Results of existing measures and proposed measure are almost in conformity with each other. From the forecasted value, it can be said that, mung productivity of India would increase to 408.84 kg/ha in 2022 as compared to 2012. In Mung, area, production and productivity Rajasthan would be leading state of India in 2022.This projection would be helpful for policy implication and planning.

Abstract Background Mung bean is a pulse crop principally grown in the tropic and subtropic parts of the world for its nutrient-rich seeds. Seven mung beans accessions from Eastern Kenya were evaluated using thirteen phenotypic traits. In addition, 10 SSR markers were used to determine their genetic diversity and population structure. This aimed at enhancing germplasm utilization for subsequent mung bean breeding programs. Results Analysis of variance for most of the phenology traits showed significant variation, with the yield traits recording the highest. The first three principal components (PC) explained 83.4% of the overall phenotypic variation, with the highest (PC1) being due to variation of majority of the traits studied such as pod length, plant height, and seeds per pod. The dendogram revealed that the improved genotypes had common ancestry with the local landraces. The seven mung beans were also genotyped using 10 microsatellite markers, eight of which showed clear and consistent amplification profiles with scorable polymorphisms in all the studied genotypes. Genetic diversity, allele number, and polymorphic information content (PIC) were determined using powermarker (version 3.25) and phylogenetic tree constructed using DARWIN version 6.0.12. Analysis of molecular variance (AMOVA) was calculated using GenALEx version 6.5. A total of 23 alleles were detected from the seven genotypes on all the chromosomes studied with an average of 2.875 across the loci. The PIC values ranged from 0.1224 (CEDG056) to 0.5918 (CEDG092) with a mean of 0.3724. Among the markers, CEDG092 was highly informative while the rest were reasonably informative except CEDG056, which was less informative. Gene diversity ranged from 0.1836 (CEDG050) to 0.5102 (CDED088) with an average of 0.3534. The Jaccards dissimilarity matrix indicated that genotypes VC614850 and N26 had the highest level of dissimilarity while VC637245 and N26 had lowest dissimilarity index. The phylogenetic tree grouped the genotypes into three clusters as revealed by population structure analysis (K = 3), with cluster III having one unique genotype (VC6137B) only. AMOVA indicated that the highest variation (99%) was between individual genotype. In addition, marker traits association analysis revealed 18 significant associations (P < 0.05). Conclusion These findings indicate sufficient variation among the studied genotypes that can be considered for germplasm breeding programs.

Background: Mung bean is important pulse crop having low productivity. Considering the constraints, it is worthwhile to study the influence of different seed hardening and foliar spraying treatments on the production potential of mung bean. Methods: Mung bean var. GAM-5 was imposed with seed hardening treatments and also for foliar spray using CaCl2 2% and 1%, CCC 500 mg/L, CCC 1000 mg/L, NAA 25 mg/L, NAA 50 mg/L during summer season of 2015-16 and 2016-17. These treatments were evaluated for their dry matter partitioning at field conditions. Result: The leaf dry matter decreased in all the treatments after 60 DAS at the time of pod development upto harvest. There was a very less rate of increase in the stem and root dry matter from pod development stage till the harvest leading to an increase in pod weight. The translocation process must have been more prominent in the treatment CaCl2 2% seed hardening + 1% spraying at 30 DAS (T11) than rest of the treatments and was less in untreated control treatment indicating that photosynthesis and translocation efficiency increases by seed hardening and foliar spraying treatments with agrochemical and growth regulators for increasing the pod yield. Therefore, the treatment T11 was more efficient in dry matter production and its partitioning in leaves, stem, root and pods by allocating maximum dry matter to the pods.

Mung bean (Vigna radiata L.) is a pulse crop with multiple uses and it was introduced recently in the study area. However, its productivity is limited by inadequate plant population and NPS fertilizer rate in the study area. Hence, this study was carried out to determine optimum plant population and NPS fertilizer rates for mung bean borda varaity in Bako, Western Ethiopia. The experiment comprised of factorial combinations of four different plant populations (500000, 571429, 666667, and 800000 plants ha-1) and five NPS fertilizer rates (0, 50, 100, 150 and 200 kg ha-1) and it was laid out using Randomised Complete Block Design with three replications. The results indicated that main effect of plant population and NPS fertilizer rates had significant effect on phenology, growth, yield, and yield components of mung bean, except stand count, above-ground biomass, straw and seed yield, which were affected by the main factors and their interactions. Highest nodule dry weight per plant (0.14g), number of pods per plant (4.74g), seeds per pod (10.26g), 100-seed weight (3.61g), and harvest index (31.16%) were observed under minimum plant population (500,000 plants ha-1). Moreover, the highest days for 50% flowering (49.08 days), 90% physiological maturity (64.5 days), effective nodules per plant (2.28),nodule fresh weight (0.33gm), nodule dry weight (0.141g plant-1),plant height (11.10cm), number of leaves per plant (8.80), number of branches per plant (3.11), tap root length (8.99cm), number of pods per plant (4.79), seeds per pod (10.78g), 100-seed weight (3.73) and harvest index (32.98%) were observed under 200 kg ha-1 NPS fertilizer. The highest stand count per hectare (780,667 plants ha−1), above-ground biomass (4,947kg ha-1), seed yield ha-1 (1,371kg ha-1) and straw yield (3,575 kg ha-1) were recorded at higher plant population (800,000 plants ha-1) with 200kg NPS ha-1 fertilizer rate (kg ha-1). However, higher plant population (800,000 plants ha-1) at the rate of 100 kg NPS ha-1fertilizer produced 1,325 kg ha-1seed yield which was the highest net benefit (50,080 ETB) and marginal rate of returns (5,610.8%). Therefore, application of 100 kg NPS ha-1 fertilizer rate with plant population of 800,000 plants ha-1 can be recommended for mung bean production in the study area and similar agro-ecologes. However, the current study was carried out only in one location for one cropping season, hence further studies over many seasons and across several locations are needed to have a conclusive recommendation for wide range of agro ecologies for mung bean production.

Background: Mung bean is important pulse crop in India due to its nutritional value. Its grain contains protein, fat, carbohydrate, vitamin and minerals therefore human uses it in various ways in food. Mung bean crop cultivated in kharif, rabi and summer season in India. Various species of insect pests are infested to mung bean crop and cause very harmful effect to crop and farmer. These pests decrease productivity and quality of mung bean. Methods: Field experiment was carried out for the study of seasonal incidence of insect pests on RMG-62 variety of green gram during kharif season 2019. Mung bean was grown on a plot size of 25 m × 25m with 50 cm row to row and 20 cm plant to plant spacing. Randomly selected 20 plants from weekly interval to record population of insect pests. The population of jassid and whitefly was recorded by counting number of pests on 2 upper, 2 middle and 2 lower leaves of a plant whereas population of aphid was recorded by counting number of aphid on 10 cm twig/plant. Result: The mean population of aphid, jassid and whitefly were recorded. Incidence of aphid started during 33th standard week with 0.9 aphid/10cm twig/plant, jassid and whitefly started during 32th standard week with 0.3 jassid/6 leaves/plant and 2.1 whitefly/6 leaves/plant, respectively. Peak mean population of aphid, jassid and whitefly reached during 36th standard week with 10.2 aphid/10cm twig/plant, 10.1 jassid/6 leaves/plant and 14.1 whitefly/6 leaves/plant, respectively. Simultaneously six species of insect pests also infested green gram, these were Mylabris pustulata, Helicoverpa armigera, Trichoplusia ni., Lampides boeticus, Spoladea recurvalis and Diaphania indica. The population of aphid, jassid and whitefly positively correlated with temperature. Population of aphid and whitefly had positive correlation with humidity, whereas jassid population had positive correlation with minimum humidity and negative correlation with maximum and average humidity. All three pests expressed negative correlation with rainfall.

Background: Intercropping is an effective tool to enhance productivity, biodiversity and stability of the fragile agro-ecosystem. In the era of shrinking resources, intercropping based on pulse crops like soybean can play an important role in crop diversification as well as agricultural sustainability. But, the information on soybean based intercropping systems is limited and needs to be explored for north-west India. Methods: A field experiment was conducted during kharif seasons of 2015 and 2016 to evaluate the effect of different soybean based intercropping systems under different planting patterns on productivity, profitability and energetics. Soybean crop was planted as sole as well as in intercropping systems viz., soybean (45 cm) + mung bean (1:1), soybean (45 cm) + cowpea fodder (1:1), soybean (45 cm) + groundnut (1:1), soybean (45 cm) + maize fodder (1:1) , soybean (60 cm) + mash (1:1), soybean (60 cm) + mung bean (1:2), soybean (60 cm) + cowpea fodder (1:2), soybean (60 cm) + groundnut (1:2), soybean (60 cm) + maize fodder (1:2) and soybean (60 cm) + maize (grain) (1:1). Result: The pooled results over two years elucidated that soybean equivalent yield was affected significantly by different intercropping systems. Soybean (45 cm) + groundnut (1:1) produced the highest soybean equivalent yield and it was significantly better than all other systems except soybean (60 cm) + groundnut in 1:2 row ratio. Similar trend was followed by production efficiency. Both, soybean (45 cm) + groundnut (1:1) and soybean (60cm) + groundnut (1:2) systems produced significantly higher energy productivity as compared to sole soybean. The highest gross (Rs. 98185 /ha) and net returns (Rs. 56122/ha) were obtained in soybean (45 cm) + groundnut (1:1) intercropping system whereas the highest benefit cost ratio was obtained in soybean (60 cm) + maize fodder (1:2) system.

Phosphorus and sulphur is a key element involved in various functions in growth and metabolism of blackgram. It is frequently a major limiting nutrient for plant growth in most Indian soils. Black gram is the major source of protein for the vegetarian diet and important pulse crop both in terms of area and production. Pulses have been in focus in recent times due to the continuous upswing in their prices. The low productivity of blackgram is due to intensive farming practices and use of exhaustive and high yield cultivars and imbalanced external fertilization. Therefore proper fertilization (site specific and balanced fertilization) is needed to increase the production and productivity of blackgram. Blackgram is very much responsive to phosphorus and sulphur application. In crop plants, the nutrient interactions are generally measured in terms of growth response, yield response and changes in concentration and uptake of nutrients. Studies have indicated synergistic effect between phosphorus and sulphur but their relationship depends on their rate of application and crop species etc. A better understanding of nutrient interaction is helpful in maximizing fertilizer use efficiency and net profit. The most of the results revealed that different levels of P and S had a significant effect on growth attributes, yield attributes, grain and haulm yield and quality prarameters of blackgram. The conjoint application up to 60 kg P2O5 ha-1 + 40 kg S ha-1 were found to be superior and synergistic in improving the growth and yield attributes of blackgram. The present paper is a critical review from research findings of eminent scientists on sulphur and phosphorus effect over black gram.

Background: The main cause for low yield in greengram is its susceptibility to Mungbean Yellow Mosaic Virus (MYMV) which is the most prevalent and destructive viral pathogen cause 85% yield lose. Inter specific hybridization between Vigna radiata and Vigna mungo could be an alternate approach to develop MYMV resistant genotypes in greengram which leads to additional source of creating variability for desirable attributes including yield, nutritional quality, biotic and abiotic stresses.Methods: The present investigation was carried out at National Pulses Research Centre (NPRC), Tamil Nadu Agricultural University, Vamban during 2016-2017. Interspecific hybridization has been effected between Vigna radiata var. VBN (Gg)2, VBN (Gg) 3 (as females) and Vigna mungo Var. Mash 114 (as male) during Summer 2016. The interspecific F1 hybrids, F2 and F3 populations were evaluated during Kharif 2016, Rabi 2016-17 and summer 2017 respectively. The F2 and F3 populations were evaluated for days to 1st flowering, days to maturity, plant height (cm), number of branches / plant, number of clusters / plant, number of pods / clusters, pod length (cm), number of seeds / pod, number of pods / plant, single plant yield (g.). The MYMV resistance has been confirmed under infector row method using CO 5 as susceptible check variety. Phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV), Heritability (h2) in broad sense, genetic advance as per cent of mean, Skewness and Kurtosis were estimated for yield and yield components. Result: Four true F1 plants were recovered in Vigna radiata var. VBN (Gg)2 x Vigna mungo Var. Mash 114 cross combination. The fertile F1 had the shallow lobbed leaf of Vigna radiata var. VBN (Gg)2 and black colour seed of Vigna mungo var. Mash 114 with pollen fertility of 42.0 per cent and crossability of 12.50%. Most interestingly all the four interspecific F1 plants were free from MYMV disease. In F2 generation, only one healthy plant was survived which had a pod, stem and branching behaviour of blackgram and greengram characters of lobbed leaf and green seed colour. In F3 generation, number of branches per plant, number of clusters per plant, number of pods per plant, pod length and seed yield per plant had high GCV, high PCV, high heritability coupled with high genetic advance as per cent of mean. Present study suggests that MYMV resistant cultivars of greengram can be explored through interspecific hybridization with Vigna mungo var. Mash 114 as a source of resistance and the hidden transgressive segregants can be recovered in F3 generation.

The study has observed an increasing trend in pulses production, driven mainly by yield improvements. The contributions of area expansion and prices to black gram growth have been erratic, suggesting that these cannot be the sustainable sources of black gram growth. Further, farmers’ area allocation decisions to pulses are not price-dependent, but depend on non price factors, mainly rainfall. However, the growth in pulses production in the long-run must come from technological changes. Numerous past studies on black gram cultivation in Tamil Nadu is criticized for using the weaker Nerlovian Partial Adjustment models and for analytical interpretation through Ordinary Least Square (OLS) creating spurious results for time series data. This problem can be avoided if Econometric technique of co-integration is used. It is for the present paper measuring the dis-Equilibrium in acreage response of black gram by using a vector error correction model. Our unit root analysis indicates that underlying data series were not stationary and are all integrated of order one, that is I(1). The Johansen co-integration approach indicates the presence of a co-integrating relationship in the acreage response model. Black gram acreage is significantly influenced by relative price of black gram, and other competing crops such as groundnut whenever resourceallocation is concerned famers preferred to allocate irrigated land to other competing crops which are more remunerative and high yielding than black gram crop. The black gram supply elasticity’s are found to be inelastic both in the short-and long-run. The long-run and short run price elasticity’s were 0.41 and 0.28, respectively.

Green gram (Vigna radiata (L.) wilczek,) commonly known as moong or mung bean or golden gram was introduced to Eritrea as a pulse crop by Ministry of Agriculture at its National Agricultural Research Institute (NARI) in collaboration with Association for Strengthening Agricultural Research in Eastern and Central Africa (ASARECA) in 2012. But its agronomic practices for semi-arid conditions of Eritrea are not yet standardised. Therefore , to find the optimum inter row spacing and phosphorus dose for its higher productivity ,a field experiment was conducted at the experimental farm of Hamelmalo Agricultural College, Keren, Eritrea during summer 2015 and 2016. The experiment was conducted in randomised complete block design (RCBD) with 12 treatment combinations of two factors consisting of four inter row spacing (Broadcast, 18cm, 30cm, and 45cm) and three phosphorus levels (0, 20 and 40 kg P2O5 ha-1) each replicated thrice. The results of the study revealed that sowing of K-26 bold seeded variety either by broadcast method or at 18cm inter row spacing at10cm plant to plant spacing fertilized with 40kg P2O5 ha-1 through DAP fertilizer drilled at the time of sowing proved significantly superior to increase growth, yield attributes and seed yield of green gram.

Background: Among pulses, blackgram is one of most important crop. Blackgram has originated from Indian sub-continent where it has been cultivated from ancient times and standout amongst most expensive pulse crop in India. Being observed, the productivity of blackgram is low in India as well as in Punjab due to lack of knowledge regarding various agronomic implementations, among them, time of sowing and selection of suitable cultivar are one of the major limiting factors in production, especially during kharif season. Methods: The research was carried out to investigate the effect of different dates of sowing on growth, yield attributes and yield of various cultivars of kharif blackgram (Vigna mungo L.) during kharif season 2019 at Student’s Research Farm, Department of Agriculture, Khalsa College, Amritsar. The experiment was laid-out in split-plot design, consisting of four dates of sowing (8th July, 18th July, 28th July and 8th August) as main-plot treatments and three cultivars (Mash114, Mash 338 and KUG 479) as sub-plot treatments, with four replications. Result: Among the sowing dates, the higher seed yield (10.27 q/ha) of blackgram was recorded when crop was raised on 8th July, which was significantly 12.6%, 20.3% and 30.5% higher than 18th July, 28th July and 8th August sown crop respectively. Among blackgram cultivars, the higher seed yield was observed in Mash 114 (10.19 q/ha) which was significantly 14% and 32% superior over Mash 338 and KUG 479 respectively. The sowing dates and cultivars had significant effect on the pods/plant, seeds/pod and seed weight/plant. The significantly higher number of pods/plant (24.3), seeds/pod (6.8) and seed weight/plant (3.2 g) were obtained with 8th July sown crop as compared to 28th July and 8th August sown crop. Among the cultivars, the significantly higher number of pods/plant (23.6), seeds/pod (6.4) and seed weight/plant (3.2 g) were recorded with Mash 114. The growth parameters viz. Plant height, nodules/plant, leaf area index and dry matter accumulation, were shown decreased trend by 29.2%, 19.13%, 12.2% and 25.4% respectively with the delay in sowing from 8th July to 8th August.

Among the important pulse-yielding plants Vigna radiata is important. In the Indian subcontinent, it is an essential source of dietary protein particularly in the vegetarian population. It contains abundant nutrients with biological activities. Soaked seeds are eaten as a good source of protein and mature seeds are cooked or can be sprouted for human consumption. Mung beans and its sprouts contain chemical constituents like flavonoids, phenolic acids, organic acids, amino acids, carbohydrates, and lipids. It is related to different biological activities like antioxidant, antimicrobial, antitumor, anti-inflammatory, anti-diabetic, and antihypertensive effects, etc. The seeds are a traditional source of cures for paralysis, rheumatism, coughs, fevers, and liver ailments. As a green manure or cover crop, the plant can be grown, enriching the soil with the nitrogen formed on its roots. The powerful Amplified Fragment Length Polymorphism (AFLP) is a molecular marker used for a variety of applications like assessing genetic diversity within species or among closely related species, inferring population-level phylogenies, generating genetic maps, and determining the relatedness among cultivars. AFLP has become extremely beneficial in the study of taxa including bacteria, fungi, and plants, where much is still unknown about the genomic makeup of various organisms. A study was made to assess the genetic diversity and phylogenetic relationships of a set of five varieties of Vigna radiata (L) Wilczek using the AFLP technique. Five different varieties of Vigna radiata viz. B1, TARM 2, PDM 84, TM 99, and TM 98 were subjected to AFLP analysis. A total of 471 fragments were scored across all the 12 AFLP primer sets used and the results were used to plot a dendrogram. It was observed that these five varieties formed three distinct groups among which the aromatic B1 variety formed a distinct group.

One of the most important basic needs for higher agricultural production is quality seed, characterized by high viability and vigour. Maintenance of seed viability and vigour from Harvest till the next growing season is of the utmost importance in a seed production programme. During seed storage, qualitative and quantitative losses of up to 20% have been reported in India. The poor seed quality may also be due to the poor storability which is very often being decided by the internal and external factors. In pulses, the major cause for seed deterioration during storage is bruchid damage. In this context, evolving an improved storage strategy to prolong the shelf life of seeds under ambient storage conditions with easily available cost-effective resources was carried out. The experiment was conducted at the Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai nagar. Freshly harvested seeds of black gram cv. ADT 3 were cleaned and graded using BSS, 8x8 wire mesh sieve. Then seeds were dried under sunlight to bring the moisture content to required level (8.5%). The seeds were treated with Thiram @ 2g kg-1, Neem oil @ 10ml kg-1 and Malathion dust @ 200 mg kg-1. Two hundred and fifty grams of seeds from all treatments along with control were packed in two containers viz. cloth bag and polythene bag (700 gauges) with three replication and stored in at ambient temperature (30o c – 35o c) and relative humidity of 52-80%. Seed samples were drawn from each replication at monthly interval for assessing the viability and vigour. Germination test was conducted in between paper method and seedlings were evaluated on seventh day for vigour index bruchid infestation. The untreated seeds recorded lower germination per cent and vigour index after seven months of storage. There was no bruchid damage on seeds treated with neem oil @ 10ml kg-1 and thiram @ 2g kg-1. It was concluded that the shelf life of black gram seeds could be increased by