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1
Malone, Laurell Coleman M. S. "The Multiple Roles of Women Pursuing Doctoral Studies." Diss., Virginia Tech, 1998. http://hdl.handle.net/10919/30544.
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Increases in the employment of women in administrative and managerial careers have drawn attention to a need for research that examines the interdependency of work and family roles, a need that is particularly crucial in the area of academic administration. This was a qualitative study of the strategies and support systems women educational administrators use to deal with the multiple roles they perform in life and work while pursuing doctoral studies.
Forty-four women educational administrators enrolled in Virginia Tech's fall 1996 dissertation seminar were selected to participate in a telephone interview. Each participant's responses were recorded and transcribed. Data were sorted using a variable-oriented format. Matrices were used to categorize and analyze the data, note emerging patterns of strategies and support systems, and compare and contrast roles across personal and situational variables.
The women in this study cited time as the common factor in most role conflicts occurring during their years of doctoral study. Strategies that centered around time management (prioritize, delegate, compartmentalize,) were used to deal with their multiple roles. Feelings of guilt, stress, exhaustion, and isolation were common. They depended on positive and affective support systems that included family, friends, co-workers, and cohort members to deal with responsibilities of home, work, and doctoral study. A strong sense of commitment, determination, and spiritual faith was credited most often as the one thing that kept them going as they responded to the problems, issues, concerns, and challenges of performing multiple roles in life and work.Ed. D.
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Sundström, Rasmus. "Upplevelsen av att dela hem och arbete med samma person." Thesis, Mälardalen University, Department of Social Sciences, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:mdh:diva-402.
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Syftet med denna studie var att undersöka hur multipla roller upplevs och hanteras av personer som delar en professionell och en privat domän. Tidigare forskning har visat att multipla roller ofta upplevs som stressande och är en grund till konflikter i såväl den privata som den professionella domänen. Åtta personer i fyra intervjupar intervjuades individuellt med hjälp av en semistrukturerad intervjuguide. Studiens resultat visar att samtliga deltagare har positiva upplevelser kring de delade domänerna då de anses ge en ökad förståelse för den andra parten och leda till en utvecklad relation. Vidare forskning föreslås koncentreras kring negativa upplevelser av fenomenet då denna undersökning inte undersökt detta.
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Kotler, Pamela L. "Having it all multiple roles and mortality /." New York : Garland Pub, 1989. http://books.google.com/books?id=whFHAAAAMAAJ.
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Voronina, Vera A. "Rx plays multiple roles in eye development." Morgantown, W. Va. : [West Virginia University Libraries], 2003. http://etd.wvu.edu/templates/showETD.cfm?recnum=2984.
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Thesis (Ph. D.)--West Virginia University, 2003.Title from document title page. Document formatted into pages; contains viii, 123 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 94-123).
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Simpson, Raina Jui Yu. "The multiple roles of zinc finger domains." Thesis, The University of Sydney, 2004. http://hdl.handle.net/2123/655.
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Zinc finger (ZnF) domains are prevalent in eukaryotes and play crucial roles in mediating protein-DNA and protein-protein interactions. This Thesis focuses on the molecular details underlying interactions mediated by two ZnF domains. The GATA-1 protein is vital for the development of erythrocytes and megakaryocytes. Pertinent to the protein function is the N-terminal ZnF. In particular, this domain mediates interaction with DNA containing GATC motifs and the coactivator protein FOG. The importance of these interactions was illustrated by the findings in Chapter 3 that naturally occurring mutations identified in patients suffering from blood disorders affect the interaction of the N-terminal ZnF with either DNA (R216Q mutation) or FOG (V205M and G208S mutations). In addition to the interaction FOG makes with GATA-1, it also interacts with the centrosomal protein TACC3. In Chapter 4, this interaction is characterised in detail. The solution structure of the region of FOG responsible for the interaction is determined using NMR spectroscopy, revealing that it is a true classical zinc finger, and characterisation of the interaction domain of TACC3 showed that the region is a dimeric coiled-coil. The FOG:TACC3 interaction appears to be mediated by a-helices from the two proteins. The data presented here represent some of the first described molecular details of how a classical ZnF can contact a protein partner. Interestingly, the a-helix used by the FOG finger to bind TACC3 is the same region utilised by DNA-binding classical zinc fingers to contact DNA. In addition to the multiple roles played by ZnFs, this domain is also known for its robustness and versatility. In Chapter 5, incomplete ZnF sequences were assessed for its ability to form functional zinc-binding domains. Remarkably, CCHX sequences (in the context of BKLF finger 3) were able to form discrete zinc-binding domains and also, mediate both protein-DNA and protein-protein interactions. This result not only illustrates the robust nature of ZnFs, it highlights the need for expanding ZnF sequence criteria when searching for functional zinc-binding modules. Together, the data presented here help further our understanding of zinc finger domains. Similar to the use of DNA-binding ZnFs in designer proteins, these data may start us on the path of designing novel protein-binding ZnFs.
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Simpson, Raina Jui Yu. "The multiple roles of zinc finger domains." University of Sydney. Molecular and Microbial Biosciences, 2004. http://hdl.handle.net/2123/655.
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Zinc finger (ZnF) domains are prevalent in eukaryotes and play crucial roles in mediating protein-DNA and protein-protein interactions. This Thesis focuses on the molecular details underlying interactions mediated by two ZnF domains. The GATA-1 protein is vital for the development of erythrocytes and megakaryocytes. Pertinent to the protein function is the N-terminal ZnF. In particular, this domain mediates interaction with DNA containing GATC motifs and the coactivator protein FOG. The importance of these interactions was illustrated by the findings in Chapter 3 that naturally occurring mutations identified in patients suffering from blood disorders affect the interaction of the N-terminal ZnF with either DNA (R216Q mutation) or FOG (V205M and G208S mutations). In addition to the interaction FOG makes with GATA-1, it also interacts with the centrosomal protein TACC3. In Chapter 4, this interaction is characterised in detail. The solution structure of the region of FOG responsible for the interaction is determined using NMR spectroscopy, revealing that it is a true classical zinc finger, and characterisation of the interaction domain of TACC3 showed that the region is a dimeric coiled-coil. The FOG:TACC3 interaction appears to be mediated by a-helices from the two proteins. The data presented here represent some of the first described molecular details of how a classical ZnF can contact a protein partner. Interestingly, the a-helix used by the FOG finger to bind TACC3 is the same region utilised by DNA-binding classical zinc fingers to contact DNA. In addition to the multiple roles played by ZnFs, this domain is also known for its robustness and versatility. In Chapter 5, incomplete ZnF sequences were assessed for its ability to form functional zinc-binding domains. Remarkably, CCHX sequences (in the context of BKLF finger 3) were able to form discrete zinc-binding domains and also, mediate both protein-DNA and protein-protein interactions. This result not only illustrates the robust nature of ZnFs, it highlights the need for expanding ZnF sequence criteria when searching for functional zinc-binding modules. Together, the data presented here help further our understanding of zinc finger domains. Similar to the use of DNA-binding ZnFs in designer proteins, these data may start us on the path of designing novel protein-binding ZnFs.
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Banga, Surinderjit. "Investigating role strain, coping and subjective well-being in combining multiple roles." Thesis, University of Plymouth, 1997. http://hdl.handle.net/10026.1/2163.
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This thesis investigates some of the salient factors involved in women's experience of combining and coping with multiple roles. A total of four studies are undertaken using both quantitative and qualitative methods to progressively focus on those factors which are identified as particularly relevant. Consequently, data is gathered using diary, interview and questionnaire methods in order to cast light upon the precise nature of the relationship between combining multiple roles, reported life satisfaction, coping and personality. In addition to identifying which particular role combinations lead to the greatest conflict, the research also clarifies the optimum number of role demands associated with reports of high life satisfaction. In so doing, the thesis provides support for the Scarcity Hypothesis as well as supplying detail about the precise nature of the role combinations associated with high and low life satisfaction. The implications of these findings for advising women on role combinations are considered. The nature of the relationship between certain key role combinations and preferred coping strategies is explored. Results suggest a relationship between certain role strains and particular coping strategies. Emotion-focused coping is preferred for inter-role conflict whereas 'superwoman' coping is more prevalent for role overload. Finally, the relationship between two major personality factors, role strain and coping, is investigated. Individuals with high scores for neuroticism are identified as expressing greater inter-role conflict, and using fewer emotion-focused coping strategies, compared with those with low scores on this scale. The implications of these findings in relation to potential social and therapeutic interventions are discussed and it is proposed that counselling and counter-conditioning might be employed to help change both cognitions and behaviour. The thesis concludes with a reflexive look at findings, issues of contention, avenues for future research, and potential practical implications.
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Xie, Xiaojun. "Multiple roles for integrins in Drosophila glial development." Thesis, University of British Columbia, 2012. http://hdl.handle.net/2429/42838.
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Glia are well known for providing essential physical and metabolic support to neurons, as well as regulating neuronal development. Glial development is also modulated by external signals from other cells and the extracellular matrix (ECM). Many signals are transduced into glia by specific receptors, such as integrins for the ECM. Previous studies show that integrins are expressed by all major vertebrate glial subtypes and play key roles in many important developmental processes. However complex composition of the integrin family and difficulties of manipulating genes in vertebrates limit the understanding of in vivo functions of integrins in glia.
Drosophila melanogaster is an excellent model for genetic analysis of the nervous system development. In this dissertation, I investigated integrin function in Drosophila glia. Integrins are expressed by glia in both the central and peripheral nervous systems at larval stages, where they form complexes with Talin and Integrin-Linked-Kinase (ILK). I found that integrin complexes were localized to different glia layers in the larval peripheral nerve and optic stalk. By using MARCM and RNA interference techniques, I found that integrins are required for multiple developmental events in individual and populated glia. In the peripheral nerve, integrins are important for glial ensheathment. When integrins were removed, perineurial glia failed to initiate or maintain their wrapping around the nerve and wrapping glia failed to send out numerous membrane processes between axons. In the optic stalk, integrins were necessary for glial migration, deposition and barrier formation. Removal of integrins impaired glial migration into the eye disc. Moreover, perineurial glia tended to aggregate at the anterior half and form multiple layers, and carpet glia failed to form organized septate junctions along the optic stalk. These glial defects resulted in photoreceptor axonal stalling in the eye disc and optic stalk, and mis-targeting in the brain. My work suggests that integrins are important for different aspects of Drosophila glial development and reveals a new glial function in helping photoreceptor axons through the optic stalk. Integrin distribution implicated that integrins may mediate glia-glia or glia-neuron interactions through ECM and non-ECM ligands.
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Donaldson, M. M. "Multiple roles of polo kinase in Drosophila melanogaster." Thesis, University of Cambridge, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.598595.
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The polo gene was originally identified as a maternal effect mutation in Drosophila. Flies homozygous for the original polo1 allele display a variety of defects in cell division throughout development that include a failure to correctly assemble centrosomes in syncytial embryos, spindle defects in the larval, CNS, chromosomal non-disjunction and failures in cytokines during male meiosis. These phenotypes are consistent with roles for PLKs (Polo-like kinases) in the separation and maturation of centrosomes to form a bipolar spindle and in cytokinesis, as reported in studies of the homologous enzymes in yeasts, Xenopus and mammalian cells. Here I describe the phenotypes of two new alleles of polo, polo9 and polo10 discovered in a screen of a collection of P-element insertions on the third chromosome of Drosophila. These alleles have defects in spindle architecture and centrosome reflecting the involvement of Polo in organising the centrosomes and the bipolar spindle. They also have a severe mitotic arrest at metaphase, which coincides with high levels of cyclin B and the continued presence of components of the spindle checkpoint mechanism. This is consistent with a role for Polo in regulating some aspects of anaphase-promoting complex (APC) function - a role in which Polo-like kinases have been implicated in other organisms, but not previously in Drosophila. I also present evidence for a synergistic interaction between the polo gene and the makos gene (which encodes Cdc27, a component of the APC). Mutations in makos enhance some phenotypes seen in larval brain cells in polo mutants. Finally I present the initial data from two yeast two-hybrid screens that use the N-terminal and C-terminal halves of Polo as baits to identify interacting proteins. This screen has highlighted new interacting molecules that will help elucidate the roles of Polo kinase.
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Graff, Tyler C. "Married Mothers' Multiple Roles: Implications for Cardiovascular Health." BYU ScholarsArchive, 2021. https://scholarsarchive.byu.edu/etd/8950.
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In recent years, the traditional nuclear family, as defined by social role theory with mother at home and father in the workplace, is no longer the norm. Nearly three out of every four women with children under the age of 18 are part of the workforce. Mothers are frequently juggling multiple roles as well as most of the responsibilities that are inherent in these roles. The current project examined diurnal ambulatory blood pressure influences associated with the responsibility of having a greater number of roles. We investigate differences between a self-reported healthy population of 112 married stay-at-home and 112 married employed mothers, all of whom have children under the age of 18 currently living in the home. Using a mixed multilevel model analysis, we found that the perception of equity in the division of childcare responsibilities between mothers and their husbands significantly contributed to lower systolic ambulatory blood pressure. We also found that married couples in relationships containing high positivity and low negativity had lower systolic ambulatory blood pressure than those which contained simultaneously high positivity and negativity. Additionally, there was a crossover interaction between these variables such that effect of relationship quality on both systolic and diastolic ambulatory blood pressure was moderated by the perception of equity in the division of childcare responsibilities between spouses. Lastly, we found that there were no ambulatory blood pressure differences between the employed and SAH mother conditions. These findings have applicable implications regarding dynamics and processes within marital relationships. These results demonstrate important social and relational influences on mothers' cardiovascular health.
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Takebayashi, Shinji. "Multiple roles of Notch signaling in cochlear development." Kyoto University, 2007. http://hdl.handle.net/2433/135772.
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Halbig, Kari Michele. "Multiple roles for the zebrafish transcriptional activator SBF/Staf." Thesis, [College Station, Tex. : Texas A&M University, 2008. http://hdl.handle.net/1969.1/ETD-TAMU-2653.
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Smithers, Lucy. "The multiple roles of delta homologues in zebrafish development." Thesis, University of Oxford, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298232.
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Hulea, Laura. "Multiple roles of CUX1 in the DNA damage response." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119486.
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The short isoforms of the CUX1 transcription factor have been implicated in various cancer-related processes in tissues culture experiments and were found to contribute to tumorigenicity in transgenic mice. In addition, CUX1 is over-expressed in many human cancers. The functions attributed so far to CUX1 stem from its activity as a transcription factor. However, it is possible that CUX1 plays a non-transcriptional role in the cell. Tandem affinity purification, followed by mass spectrometry, identified numerous binding partners of CUX1. The aim of my studies was to validate and further characterize the interactions of CUX1 with some of these proteins: REV1, PARP1 and Ku70/Ku80. REV1 is an error-prone DNA polymerase involved in translesion synthesis and is responsible for most point mutations in yeast and mammalian cells. We showed that the REV1-CUX1 interaction required the REV1 BRCT domain and was increased following irradiation. I have shown that CUX1 was phosphorylated following DNA damage. My results indicated that ATM phosphorylated CUX1 and that phosphorylation of CUX1 at serines 861, 868 and 1100 promoted its interaction with REV1. ChIP-on-chip experiments showed that REV1 preferentially localized to transcribed regions, that the recruitment increased after gamma-irradiation and that 12.9% of REV1 binding sites overlapped CUX1 binding sites. I have optimized and characterized two reporter assays for point mutations (GFPstop and ouabain resistance) and showed that point mutation frequency correlated, at least in part, with the ability of a promoter to recruit REV1. Reduction in REV1 expression led to a decrease in expression of numerous genes. We propose that the physiological purpose for REV1 recruitment to transcribed regions is to enable efficient transcription of actively transcribed genes, by preventing stalled replication forks from blocking the passage of the transcription machinery. Ku and PARP1 are sensors of DNA damage and promote DNA damage repair. I have validated the interaction of CUX1 with Ku and PARP1 and showed that expression of CUX1 fragments led to their displacement from known promoter targets. Using various experimental approaches, my results indicated that in addition to its transcriptional role, CUX1 might play a non-transcriptional role in the repair of strand breaks.Le facteur de transcription CUX1, et plus particulièrement ses isoformes courtes, sont impliquées dans différents processus associés au développement cancéreux dans des expériences de culture de tissus et dans des modèles de souris transgéniques. De plus, CUX1 est sur-exprimé dans de nombreux cancers humains. Les fonctions attribuées jusqu'à présent à CUX1 sont associées à son activité transcriptionelle. Cependant, il est possible que CUX1 joue un rôle non-transcriptionel dans la cellule. La purification par affinité en tandem, couplée à la spectrométrie de masse, a permis d'identifier plusieurs partenaires d'interaction de CUX1. Le but de mes études a été de valider et caractériser les interactions de CUX1 avec certaines de ces protéines, plus précisément REV1, PARP1 et Ku70/Ku80. REV1 est une ADN polymérase de faible fidélité lors de la synthèse d'ADN impliqué dans le processus de synthèse à travers les lésions (translesion synthesis) et responsable de la plupart des mutations ponctuelles chez la levure, ainsi que chez les mammifères. Nous avons montré que l'interaction entre REV1 et CUX1 nécessitait le domaine BRCT de REV1 et que leur affinité respective augmentait suite à l'irradiation. J'ai montré que CUX1 était phosphorylé suite aux dommages à l'ADN. Mes résultats ont indiqué que ATM phosphorylait CUX1 et que les phosphorylations de CUX1 sur les serines 861, 868 et 1100 favorisaient son interaction avec REV1. Des expériences de localisation génomique par puce ont montré que REV1 était localisé de manière préférentielle dans des régions transcrites, que cette localisation augmentait suite à l'irradiation aux rayons gamma et que 12.9% des sites de localisation de REV1 se superposaient aux sites de localisation de CUX1.J'ai optimisé et j'ai caractérisé deux essais rapporteurs pour l'analyse des mutations ponctuelles (GFPstop et résistance à l'ouabain) et j'ai montré que la fréquence des mutations ponctuelles corrélait, en partie, avec la capacité des promoteurs à recruter REV1. Une réduction de l'expression de REV1 a induit la réduction de l'expression des nombreux gènes. Nous suggérons que le rôle physiologique du recrutement de REV1 au sein de régions génomiques transcrites est de permettre la transcription efficace des gènes transcrits activement, en prévenant le blocage du passage du complexe transcriptionel normalement induit par les fourches de réplication arrêtées à cause du dommage à l'ADN. Ku et PARP1 sont des détecteurs du dommage à l'ADN et stimulent la réparation de l'ADN. J'ai validé l'interaction de CUX1 avec Ku et PARP1 et j'ai montré que, suite à l'expression des fragments de CUX1, Ku et PARP1 ne se localisaient plus sur certaines de leurs cibles génomiques. En utilisant différentes approches expérimentales, mes résultats ont indiqué que, en plus de son rôle transcriptionel, CUX1 pourrait jouer un rôle non-transcriptionel dans la réparation des cassures de l'ADN.
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Dickens, Kristen N. "Counselor Education Doctoral Students' Experiences with Multiple Roles and Relationships." ScholarWorks@UNO, 2014. http://scholarworks.uno.edu/td/1789.
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The purpose of this phenomenological study was to explore the lived experiences of counselor education doctoral students who participated in multiple roles and relationships. Random purposeful sampling was used to conduct in-depth interviews with current doctoral students in CACREP-accredited counselor education programs who had completed at least one year of full-time enrollment as a doctoral student, participated in a minimum of two multiple roles that were provided in an a priori list, and had access to videoconferencing software in order to participate in the study.
The participants in this study reported and described perceptions of their lived experiences as counselor education doctoral students. The primary research question for the study was “How do counselor education doctoral students experience the phenomenon of multiple roles and relationships?” A review of the literature that examined types of multiple roles and relationships between counselor educators and students, ethical standards, and models for ethical management provided the foundation for the study. Semi-structured phenomenological interviews comprised of open-ended questions were used to collect data via videoconferencing software. Audio taped interviews were transcribed and analyzed for key words and descriptive terms. The data were coded into categories, categories were clustered into themes and themes were cross-analyzed to create super-ordinate themes. Super-ordinate themes were used to address the primary and secondary research questions.
Three super-ordinate themes emerged: awareness and education, multiple roles and relationships as transformative, and experiential learning. Implications for counselor education doctoral students and programs are presented along with recommendations for further research. Personal reflections of the researcher were provided.
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Fillpot, Cynthia Ann. "Role conflict and hardiness as predictors of role and life satisfaction for women occupying multiple roles." CSUSB ScholarWorks, 1994. https://scholarworks.lib.csusb.edu/etd-project/900.
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Yoda, Mitsumasa, Tomohiro Ushikubo, Wataru Inoue, and Masaki Sasai. "Roles of noise in single and coupled multiple genetic oscillators." American Institute of Physics, 2007. http://hdl.handle.net/2237/8798.
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Mohamed, Othman. "Identification of multiple roles for Wnt signaling during mouse development." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=85087.
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Signaling molecules play essential roles in communication between cells. Wnt signaling molecules are critical for embryonic development of several organisms. I examined the involvement of Wnt signaling during two major developmental processes, namely embryo implantation and formation of the embryonic body axes. Using RT-PCR analysis, I showed that multiple Wnt genes are expressed in the blastocyst at the time of implantation. Moreover, expression of Wnt 11 requires both estrogen produced by the mother and the uterine environment. Using a transgenic approach, I showed that beta-catenin-regulated transcriptional activity, which is a major transducer of Wnt signaling, is activated in the uterus specifically at the site of implantation in an embryo-dependent manner. These results introduce Wnts as candidate signaling factors that may mediate the communication between the embryo and uterus that initiates implantation.Wnt/beta-catenin signaling triggers axis formation in Xenopus and zebrafish embryos. I showed that, during embryonic development, beta-catenin-regulated transcriptional activity is first detected in the prospective primitive streak region prior to gastrulation. This demarcates the posterior region of the embryo. This activity then becomes restricted to the elongating primitive streak and to the node. In Xenopus embryos, beta-catenin participates in the formation of the organizer through the activation of the homeodomain transcription factors Siamois and Twin. I obtained evidence that a Siamois/Twin-like binding activity exists in mouse embryos and is localized in the node. These results strongly suggest that, as the case in Xenopus and zebrafish, the Wnt/beta-catenin pathway is involved in establishing embryonic body axes.
Furthermore, using the transgenic mouse line that I generated for these studies, I mapped the transcriptional activity of beta-catenin during mouse embryonic development. These results revealed when and where this activity, and presumably Wnt signaling, is active during the development of several organs and embryonic structures.
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Anderson, Victoria Elizabeth. "Sfi1p has multiple roles in the spindle pole body cycle." Thesis, University of Edinburgh, 2006. http://hdl.handle.net/1842/13440.
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In order to identify new mitotic defects a screen for mutants synthetic lethal with a mad1Δ deletion strain was performed in S. cerevisiae. Mad1p is a component of the spindle assembly checkpoint. Four of the mutants isolated in the screen were novel mutations in the essential sfi1 gene. In this study, Sfi1p is shown to be a spindle pole body (SPB) protein that contains a conserved set of 211 amino acid repeats. Its phosphorylation is cell cycle regulated, with most phosphorylation being present in an alpha factor (G0/g1) arrest, the time of spindle pole body duplication. The mutants from the screen all had mutations C-terminal to the conserved 21 amino acid repeats. This C terminal region is not conserved outwith the budding yeast, but within the budding yeasts family is the most conserved region. Deletion of the C terminal region is lethal, and results in mis-localisation of the protein. The sfi1-CT mutants are lethal with a range of spindle checkpoint proteins, indicating that the defect is recognised by the spindle assembly checkpoint. At all temperatures the sfi1-CT mutants showed varying levels of large budded cells delayed in mitosis with spindle pole bodies very close together (0.2 – 0.4 μm) and few normal metaphase (1-1.15 μm) spindles. Examination of these abnormal SPB pairs by electron microscopy revealed many large budded cells containing sets of paired spindle pole bodies still attached by a half bridge, as well as some that had partially separated and had some microtubules between them. In all cases, both spindle pole bodies appear morphologically normal and can nucleate nuclear and cytoplasmic microtubules. This is in contrast to other Sfi1p mutants in the conserved repeats that result in a failure of spindle pole body duplication, and suggests that Sfi1p has multiple functions in both SPB duplication and separation.
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Benedykcinska, A. M. "Multiple roles of β-catenin in brain development and tumourigenesis." Thesis, University College London (University of London), 2014. http://discovery.ucl.ac.uk/1436079/.
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β-catenin is a multifunctional protein with roles in Wnt pathway signal transduction and in cell adhesion. While the normal function of β-catenin is important for CNS development, Wnt pathways have also been intrinsically linked to cancer. Here, the multiple roles of β-catenin in CNS development and in brain tumour pathogenesis were investigated. All experiments used a mouse model where a dominant active form of β-catenin can be induced by Cre mediated recombination of the exon 3 of the CATNNB gene. Three models were established to analyse the effects of dominant active β-catenin: (i) during development with En2Cre, in the midbrain-hindbrain regions, (ii) in mature (L7Cre) Purkinje neurons, and (iii) in adult stem cells using Cre delivery into the SVZ using an established and a specifically developed novel method. En2Cre;β‐cateninlox(ex3) mice express mutant β-catenin in the midbrain-hindbrain junction during early brain development. Although En2 is expressed between E8 and E12.5, the precise timing and duration of stabilized β‐catenin is not known. At later stage, these mice showed decreased motor performance, caused by a significant defect in the vermis region. This phenotype could be rescued by deletion of p53 gene, pointing at a potential role of p53/β-catenin cross-talk. In contrast, constitutive activation of β-catenin in mature cerebellar Purkinje neurons using the L7Cre; β-cateninlox(ex3) model, does not cause cell death or dysfunction of these neurons. To target SVZ stem/progenitor cells, we used an established model with adenovirus-Cre mediated recombination and we developed a highly selective approach through direct, intraventricular injection of Endoxifen into GLAST-Cre(ERT2) mice. Both approaches resulted in similar phenotypes and latencies to tumour development, and required at least one additional tumour suppressor to be inactivated simultaneously to cause brain tumours. The results suggest that β-catenin has diverse effects during different developmental stages. During early development, it causes widespread cell death, whilst no effect is seen in mature cells. In adult SVZ progenitor cells it has no effect unless tumour suppressor genes such as p53 or PTEN are concomitantly inactivated, resulting in formation of brain tumours.
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KINZIG, KIMBERLY PEACOCK. "MULTIPLE ROLES OF THE CNS GLUCAGON-LIKE PEPTIDE-1 SYSTEM." University of Cincinnati / OhioLINK, 2002. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1037717933.
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Liang, Debbie T. "Multiple roles of fission yeast MCM proteins in DNA replication /." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2001. http://wwwlib.umi.com/cr/ucsd/fullcit?p3041400.
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Dobosiewicz, Ilona Harris Victoria Frenkel. "Redefining womanhood multiple roles of female relationships in Jane Austin's novels /." Normal, Ill. Illinois State University, 1993. http://wwwlib.umi.com/cr/ilstu/fullcit?p9323731.
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Thesis (D.A.)--Illinois State University, 1993.Title from title page screen, viewed February 9, 2006. Dissertation Committee: Victoria Frenkel Harris (chair), Richard Dammers, Charles Harris, William Morgan. Includes bibliographical references (leaves 244-255) and abstract. Also available in print.
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Ramakrishnan, Nitya. "Characterizing the roles of multiple Gbetagamma binding sites on Kir3 channels." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:8881/R/?func=dbin-jump-full&object_id=92394.
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Elie-Dit-Cosaque, Christophe. "Studies on Adaptation to Information Systems: Multiple Roles and Coping Strategies." Digital Archive @ GSU, 2009. http://digitalarchive.gsu.edu/cis_diss/37.
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Understanding individual adaptation to Information Systems (IS) has received relatively little attention in IS research. For furthering these issues, a multi-paper dissertation is adopted and studies distinct aspects of user interaction with IT related with adaptation. Thus, in order to better understand how system users adapt to IT disruptions this study examines (1) how system users who become disrupted by IS that provide them with too much information interact with these systems, (2) the influence of espoused cultural values (Srite et al. 2006) on user coping strategies of adaptation to IS, and (3) middle managers responses to the implementation of disruptive IT in public administration. These dissertation studies together help improve our knowledge on individual adaptive responses to IT disruptions.
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Oduwo, Elizabeth. "Understanding the multiple roles for the state in HIV vaccine research." Thesis, McGill University, 2010. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=86994.
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Kenya has a rich and controversial history of HIV vaccine-related research and incidents between researchers, participants and authorities have highlighted pertinent and endemic ethical, legal, social and political issues. The role of the state during these events and in wider vaccine research is unexamined and misunderstood; yet, the state is a deeply involved and dominant party.My thesis provides a coherent explanation of the role of the state in vaccine research and links recurrent ethical issues to the multiple and competing interests the state has in this activity. I develop multiple roles for the state as a Facilitator, Guardian, Participant, Regulator, Researcher and Sponsor based on a common understanding of the key parties in biomedical ethics. These roles explain the complex state participation and are developed and shaped by crucial influential factors in the environment surrounding HIV vaccine research.
Le Kenya a une histoire riche et controversée à l'égard de la recherche relative au vaccin contre le VIH. En effet, plusieurs incident et conflits entre les chercheurs, les participants et les autorités mettent en évidence les difficultés éthiques, légales, sociales et politiques de la recherche d'un vaccin. Le rôle de l'Etat au cours de ces évènements et en ce qui a trait a la recherche de vaccin en général est non examine et mal compris, et pourtant, l'Etat est une partie profondément impliquée et dominante.
Ma thèse propose une explication cohérente du rôle de l'E tat dans la recherche d'un vaccin et relie les difficultés éthiques récurrentes aux multiplies intérêts concurrents que l'Etat détient a l'egard de cette activité. En se basant sur une compréhension commune des principales parties impliquées en éthique biomédicale, je développe de multiples rôles pour l'Etat en tant que facilitateur, gardien, participant, régulateur, chercheur et mémé commanditaire de l'activité. Ces rôles, expliquant la participant complexe de l'Etat sont façonnes par des facteurs cruciaux et influents provenant de l'environnement qui entoure la recherche d'un vaccin contre le VIH. En effet, l'implémentation de ces rôles créé un environnement propice aux conflits et aux difficultés éthiques.
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Heine, Danielle Lynn. "Multiple roles of Wnt6 during Xenopus organogenesis : heart, kidney and eye." Thesis, University of Aberdeen, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.439976.
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Using stage-specific over-expression in transgenic Xenopus embryos, I show that both Wnt6 and β-catenin can negatively regulate several molecular markers for heart muscle differentiation as well as earlier heart-specific transcription factors. In addition, Wnt6 is required for proper heart development since knockdown of XWnt6 results in a non-beating heart and up-regulation of several of these myocardial markers. Complementary to this finding, activation of canonical Wnt signalling or over-expression of mouse Wnt6 inhibits mouse embryonic stem cell differentiation into beating cardiomyocytes. Wnt6 functions to restrict heart muscle differentiation. Due to Wnt6 expression within the pronephritic anlage and later within the pronephritic tubules, and because this kidney specific expression is conserved in mouse where Wnt6 is expressed in the ureter bud, we investigated an additional role for XWnt6 in kidney development. Knockdown of Wnt6 studies in Xenopus show that Wnt6 is clearly required for pronephros development since loss of XWnt6 function results in loss of expression of a number of pronephros-specific genes including many associated with MET such as Na+ K+ATPase and NKCC2 which are required for proper tubule formation and function. Therefore, XWnt6 is not required for those genes that require XWnt4 expression and it appears instead that these two Wnt proteins work in parallel to direct expression of different genes required for kidney development. In addition, both gain and loss of XWnt6 function result in eye phenotype and XWnt6 function is required for expression of the proneural gene, Sox2. Furthermore, it appears likely that XWnt6 signals with the Wnt receptor Frizzzled5 to regulate expression of Sox2 in the developing retina to promote proper eye formation (Van Raay, Moore et al. 2005). In summary, XWnt6 restricts heart muscle differentiation whereas it is a positive regulator of kidney and eye development.
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Elie-dit-Cosaque, Christophe Max-Olivier. "Studies on adaptation to information systems : Multiple roles and coping strategies." Paris 9, 2008. https://portail.bu.dauphine.fr/fileviewer/index.php?doc=2008PA090038.
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L’adaptation individuelle aux technologies de l’information (TI) a reçu relativement peu d’attention dans la recherche en systèmes d’information (SI). Afin de contribuer à une meilleure compréhension des problématiques que soulève ce concept, un format de thèse sur travaux est adopté. L’objectif est d’analyser des aspects distincts de l’interaction des utilisateurs avec les TI en lien avec la notion d’adaptation. Par conséquent, afin de mieux comprendre comment les utilisateurs de TI s'adaptent à ces dernières, cette étude examine (1) l’interaction d’utilisateurs avec une TI qui leur fournit différentes quantités d’information, en lien avec le succès de cette TI (DeLone et McLean 1992) (2) l’influence des valeurs culturelles épousées (Srite et Karahanna 2006) d'individualisme-collectivisme et d’évitement de l’incertitude sur les stratégies d’adaptation et de coping des individus lors de l’implémentation d’une TI disruptive, (3) et, partant d’une conceptualisation basée sur les études d’Emirbayer et Mische (1998) et Crozier et Friedberg (1977), les actions prises par des cadres intermédiaires lors de l’intégration d’une TI disruptive dans les unités locales d’une administration publique. Pour répondre aux questions de recherche qui sont posées, ces études adoptent des méthodologies quantitatives expérimentales (études 1 et 2) et qualitative (étude 3) et entendent contribuer à améliorer notre compréhension des réponses des individus aux changements liés à l’introduction des TIUnderstanding individual adaptation to Information Systems (IS) has received relatively little attention in IS research. For furthering these issues, a multi-paper dissertation is adopted and studies distinct aspects of user interaction with IT related with adaptation. Thus, in order to better understand how system users adapt to IT disruptions this study examine (1) how system users who become disrupted by IS that provide them with too much information interact with these systems, (2) the influence of espoused cultural values (Srite and Karahanna 2006) on user coping strategies of adaptation to IS, and (3) middle managers responses to the implementation of disruptive IT in public administration. These dissertation studies together help improve our knowledge on individual adaptive responses to IT disruptions
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Megumi, Yuzuru. "Multiple roles of Rbx1 in the VBC-Cul2 ubiquitin ligase complex." Kyoto University, 2006. http://hdl.handle.net/2433/144318.
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Dengler, Sarah Hart. "Distinct roles for Foxo1 at multiple stages of B cell differentiation." Diss., Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2008. http://wwwlib.umi.com/cr/ucsd/fullcit?p3320954.
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Thesis (Ph. D.)--University of California, San Diego, 2008.Title from first page of PDF file (viewed October 3, 2008). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 69-83).
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Lorgeoux, Rene-Pierre. "Multiple roles of DDX17 in human immunodeficiency virus type 1 replication." Thesis, McGill University, 2013. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=119579.
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Human immunodeficiency virus type 1 (HIV-1) is a small retrovirus that highly depends on the host cell machinery to replicate by completing its lifecycle and producing new infectious viral particles. The complexity of HIV-1 life cycle regulation only reflects the diversity of the virus-host interactions. Cellular helicases are enzymes involved in every step of nucleic acid metabolism, through rearranging ribonucleoprotein complexes. The understanding and the importance of helicases in HIV-1 replication started to emerge a decade ago. Since then, many studies reported the promoting or inhibiting effect of this protein family on HIV-1. My thesis project was to investigate the role of helicases in HIV-1 replication and comprises two parts. First, we performed a shRNA screen in SupT1 cells to knockdown 130 helicases and monitor their effect on the production of HIV-1 particles. This work allowed us to identify cellular pathways that are important for HIV-1 replication, as well as 35 potential helicases that dramatically affect virus production. Second, we chose to further investigate the role of DDX17 in HIV-1 replication. In addition to showing for the first time that a helicase is required for HIV-1 frameshift, we found that DDX17 promotes viral RNA packaging. Considering the role of DDX17 as a cofactor of the zinc antiviral protein (ZAP) in exosome-mediated HIV-1 mRNA degradation, this emphasizes the fact that helicases are multifunctional proteins. Finally, this work identifies helicases that potentially strongly modulate HIV-1 production. Individual investigation for each candidate will be needed to unravel the mechanisms underlying their effect on HIV-1 replication.Le virus de l'immunodéficience humaine de type 1 (VIH-1) est un petit rétrovirus qui dépend fortement de la machinerie cellulaire afin de compléter son cycle de réplication et produire de nouvelles particules virales infectieuses. La complexité de la régulation du cycle de réplication du VIH-1 reflète la diversité des interactions hôte-virus. Les hélicases sont des enzymes impliquées dans toutes les étapes du métabolisme des acides nucléiques, en réarrangeant les complexes ribonucléprotéiques. La compréhension de l'importance des hélicases dans la réplication du VIH-1 a commencé il y a une dizaine d'années. Depuis, plusieurs études ont rapporté les effets stimulateurs ou inhibiteurs de cette famille de protéines sur le VIH-1. Mon projet de thèse était d'investiguer le rôle des hélicases dans la réplication du VIH-1 ; il comprenait deux parties. Premièrement, nous avons supprimé l'expression de 130 hélicases au moyen de shRNAs dans les cellules SupT1. Ce travail nous a permis d'identifier les voies cellulaires majoritairement impliquées dans la réplication du VIH-1, ainsi que 35 hélicases affectant de manière drastique la production virale. Dans un second temps, nous avons choisi de nous intéresser plus en détails au rôle de la protéine DDX17 dans la réplication du VIH-1. En plus d'identifier pour la première fois une hélicase étant requise pour le décalage du cadre de lecture (-1), nous montrons que DDX17 favorise l'encapsidation de l'ARN viral. Considérant que DDX17 agit également en tant que co-facteur de ZAP (protéine antivirale zinc) dans la dégradation des ARNs du VIH-1 par l'exosome, cela souligne le fait que les hélicases sont multifonctionnelles. Finallement, au cours de ce travail nous avons identifié un certain nombre d'hélicase ayant le potentiel de fortement moduler la production du VIH-1. Des études individuelles seront nécessaires afin de mettre à jour les mécanismes responsables de l'effet de chacun des candidats sur la réplication du VIH-1.
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Quach, Emma D. "Multiple Roles in Later Life| Role Enhancement and Conflict and Their Effects on Psychological Well-Being." Thesis, University of Massachusetts Boston, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10239615.
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Holding both work and family roles can be a central experience for men and women, young or old. Yet, to date, the bulk of knowledge on holding roles in both domains is specific to young adults, a critical gap as conditions warrant longer work life. This inquiry thus focused on older working men and women (over 50 years of age) with at least one family role (spouse, parent of adult children, caregiver to an aging parent, or grandparent). With survey data from the Health and Retirement Study in 2010 and 2012, latent profile analysis, path analyses, and regressions were conducted to investigate multiple roles in later adulthood: 1) The extent older workers experience role enhancement and conflict between work and family roles because of role stressors and rewards, and patterns of role enhancement and conflict experiences, 2) The extent role enhancement and conflict (a) mediate between role rewards/stressors and psychological well-being (aging self-perceptions, life satisfaction, and depressive symptoms), and (b) interact with each other when exerting their psychological impacts, 3) Gender differences in role enhancement and conflict experiences and in their psychological consequences. Holding multiple roles in later life was characterized predominantly by work and family roles mutually enhancing each other, rather than conflicting with each other, a pattern driven primarily by low role stressors and secondarily by high role rewards. Role enhancement and conflict mediated the effects of role stressors/rewards on psychological well-being, especially on self-perceptions on aging. Interactive effects were also found: Psychological well-being was fostered by work conflicting with and enhancing the family but compromised by a similar circumstance in the family. Finally, gender differences emerged. Women benefitted more than men from multiple sources of role enhancement and from their work role (even when it enhanced and conflicted with the family). Men’s psychological well-being was neutral to multiple sources of role enhancement, enhanced by multiple sources of role conflict, and compromised by later-life family (when it enhanced and conflicted with work). In conclusion, although men and women experienced multiple roles in unique ways, they overwhelmingly benefitted from socially recognized activities from work and family roles.
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Rebelo, de Andrade Goncalo N. N. S. "Khd1p, a protein with multiple roles in mRNA localization and telomeric silencing." Diss., lmu, 2008. http://nbn-resolving.de/urn:nbn:de:bvb:19-89346.
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Doyle, Alexander Edward. "Myo51, the fission yeast type V myosin with multiple roles during meiosis." Thesis, University of Kent, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508317.
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Erickson, Drew Talyn. "Multiple Roles for the Transcription Factors Sox6 and Jumonji in Mouse Hematopoiesis." Diss., The University of Arizona, 2006. http://hdl.handle.net/10150/195728.
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Sox6, a member of the Sox transcription factor family, is essential for the silencing of epsilon-y-globin gene expression in definitive erythropoiesis of mice and humans. Homozygous Sox6 null mice are neonatal lethal, precluding analysis at later stages. We created adult mice that are deficient in Sox6 specifically in hematopoietic tissues, by transplanting embryonic liver stem cells from Sox6-deficient mice into lethally-irradiated congenic wild-type adult mice. The mice receiving mutant stem cells (mutant-engrafted) showed high expression levels of epsilon-y in bone marrow, spleen and circulating blood compared to mice receiving wild-type and heterozygous stem cells (control-engrafted). The level of expression of epsilon-y in circulating blood was directly correlated with the percentage of successful mutant donor cell engraftment. Additionally, the mutant-engrafted adult mice showed an increase in erythroid precursor cells in bone marrow, spleen and blood. Thus, Sox6 continues to function as a major regulator of epsilon-y in adult definitive erythropoiesis and is required for normal erythrocyte maturation. Moreover, Sox6 may provide a novel therapeutic target by reactivating epsilon-y in patients with hemoglobinopathies such as sickle cell anemia and beta-thalassemia.We have also identified another transcription factor, jumonji, as a downstream target of Sox6. Jumonji is a crtitical transcription factor in neural, cardiac and erythroid development. We report here that jumonji is over-expressed in the fetal liver of Sox6-deficient mice (p100H/p100H). Transfection assays in H2.35 cells reveal that a ~1.6-kb genomic fragment, including the 5' UTR of jumonji, contains both promoter activity and Sox6-mediated repression. Chromatin immunoprecipitation and electromobility shift assays demonstrate that Sox6 binds to a region within the second exon of jumonji. Further transfection analyses confirm that one of five putative binding sites for Sox6 in this region is required for the majority of Sox6-mediated transcriptional repression. In irradiated mice engrafted with Sox6-deficient hematopoietic stem cells, jumonji expression levels are significantly elevated in blood and bone marrow. These results demonstrate that Sox6 plays a major role in the direct repression of jumonji transcription, and it is likely that jumonji plays a cell-autonomous role in the subsequent hematopoietic cell phenotype seen in Sox6-deficient mice.
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Providence, Cheryl Jepsen. "Effects of instrumentality and expressiveness on women's preferences for multiple life-career roles." Virtual Press, 1993. http://liblink.bsu.edu/uhtbin/catkey/897474.
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Based on Super's (1990) developmental model of career development and Spence and Helmreich's (1978) gender identity theory, this study examined the relationship between the gender-related traits of instrumentality and expressiveness and women's preferences for multiple life-career roles. Super (1990) suggested that a career, as represented by the major life roles of student, worker, citizen, homemaker, and leisurite, is influenced by sex role stereotyping and individual differences. It was hypothesized in this project that gender role orientation (levels of instrumentality and expressiveness) would have an effect on women's role preferences.Adult women (N = 100) from three medium-sized midwestern communities were recruited from churches and community sororities. The women ranged in age from 20 to 82, with a mean age of 44.8 years. A response rate of 53%% was obtained. Role preferences, as expressed by women's participation, commitment, and value expectations, were measured by Nevill and Super's (1986) Salience Inventory (SI). Gender role orientation was assessed by Spence and Helmreich's (1974) Personal Attributes Questionnaire (PAQ). Scores on the PAQ were grouped into the categories ofandrogynous, instrumental, expressiveness, and undifferentiated by the median split technique. A demographic questionnaire was also administered.The demographic variables were grouped conceptually into five categories: personal, occupational, role satisfaction, parental influence, and spousal support for the purpose of preliminary analyses. Results of these analyses (Pearson Productmoment correlations, canonical correlations, and discriminant function analyses) revealed that personal and role satisfaction variables may moderate women's gender role orientation and role preferences. The main analyses were then conducted with three separate between-subjects MANOVAs. Although the results of the MANOVAs involving women's participation and commitment to multiple life-career roles were nonsignificant, another MANOVA involving women's value expectations was significant. Post-hoc procedures indicated that androgynous women had greater expectations of achieving their values in their work role than did instrumental women. It was also found that androgynous women had higher value expectations in the community and home roles than did undifferentiated women.A number of theoretical, empirical, and counseling implications were discussed. Limitations of the study were discussed in terms of the sample characteristics and the statistics employed.Department of Counseling Psychology and Guidance Services
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Gale, Emily Anne. "Multiple roles for retinoic acid in the development of the chick nervous system." Thesis, King's College London (University of London), 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.244287.
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Aktar, Rubina. "Multiple roles for the extracelllular matrix protein Tenascin-X in nerve gut function." Thesis, Queen Mary, University of London, 2016. http://qmro.qmul.ac.uk/xmlui/handle/123456789/18406.
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Tenascin X (TNX) is a matricellular protein involved in regulating cellular functions by interacting with other extracellular matrix (ECM) proteins within the cell matrix and has anti-adhesive properties evidenced in tumours and wound healing. TNX is the only member of the tenascin family that is lost in Joint Hypermobility Syndrome (JHS) and exerts a crucial architectural function. Of importance, TNX deficient and JHS patients have gastrointestinal (GI) dysfunction. Despite this association no study has described the role of TNX in the GI tract. Thus, the aim of this thesis was to characterise the expression of TNX in the stomach and colon in mouse and human tissue. Second, we aimed to elucidate the functional role of TNX using TNX knockout (TNX KO) mice. Expression studies revealed TNX in vagal afferent endings in the mouse, and myenteric cell bodies in human stomach. In colon, TNX strongly associated with cholinergic submucous and myenteric neurons in both species, however, was not found in CGRP positive fibres. Cell bodies in nodose ganglia, dorsal root ganglia, ventral and dorsal horn were also TNX positive. Functional studies in stomach, using single fibre electrophysiology showed TNX KO mice had increased vagal afferent mechanoreceptor sensitivity. Octanoic acid breath test revealed rapid gastric emptying in TNX KO. Colonic manometry showed the amplitude and frequency of colonic contractions were reduced in TNX KO mice, particularly in the distal colon. Ussing chamber studies measuring changes in ion flux (indirect measure of secretion) showed no major difference between TNX KO and wild type (WT) mice. The specific localisation of TNX with neuronal structures in the gut is shown here for the first time suggesting that TNX is more than just an architectural protein. Indeed, its role in specific GI functions supports this observation and provides a mechanism for GI symptoms in JHS.
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Pierson, Frank William. "The roles of multiple infectious agents in the predisposition of turkeys to colibacillosis." Diss., Virginia Tech, 1993. http://hdl.handle.net/10919/29318.
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Colibacillosis is considered one of the more costly diseases encountered in the production of market turkeys. It is responsible for a significant amount of mortality in birds between the ages of 6-12 weeks.
Research conducted over the past 5 years has shown that within the Shenandoah Valley production area, multiple primary infectious agents are responsible for the predisposition of turkeys to colibacillosis. These agents were first identified as potential contributors through field case studies. They include hemorrhagic enteritis (HE) virus, Newcastle disease virus, and Bordetella avium. Further retrospective serologic studies affirmed the role of these primary agents and uncovered the potential involvement of Mycoplasma meleagridis.
Trials were conducted to determine the reproducibility of some multiple agent interactions under laboratory conditions. It was found that Newcastle disease virus or B. avium infection followed by HE virus and Escherichia coli challenge produced clinical colibacillosis.
It is believed that hemorrhagic enteritis virus is the pivotal agent in this process of predisposition. Almost all turkeys are vaccinated for hemorrhagic enteritis in the field. The virulent strains of the virus are known to be immunosuppressive. It is suspected that the vaccine strains are mildly so. Infection with HE vaccine virus was shown to cause an increase in CT8+ cells in peripheral blood. These cells are believed to be suppressor T-cells and may account for the reputed immunosuppressive effects of the virus.
Thus, interactions of multiple infectious agents including Newcastle disease virus, B. avium, M. meleagridis, and HE virus appear to be involved in the predisposition of turkeys to secondary E. coli infections.Ph. D.
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Christensen, Kimberly Laura. "The developmental regulator SIX1 plays multiple roles in breast cancer initiation and progression /." Connect to full text via ProQuest. Limited to UCD Anschutz Medical Campus, 2007.
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Thesis (Ph.D. in Biophysics & Genetics, Program in Molecular Biology) -- University of Colorado Denver, 2007.Typescript. Includes bibliographical references (leaves 115-132). Free to UCD affiliates. Online version available via ProQuest Digital Dissertations;
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Chu, Moong Li. "The Role of Work-Study Boundary Congruence in the Study and Life Outcomes of Working Student." Thesis, Griffith University, 2018. http://hdl.handle.net/10072/382730.
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Boundary congruence, which is defined as the match between the person’s role management preferences and the constraints of the environment, is associated with work-study conflict, work-study facilitation (Butler, 2007), well-being (LeComte-Hinely, 2013; Li, Wang, You, & Gao, 2015), and academic performance (Butler, 2007). Boundary congruence is a role management process (Kreiner, Hollensbe, & Sheep, 2009), which is especially important for university students who are working while studying (Butler, 2007); that is, have multiple roles to manage and balance in their lives (Lingard, 2012). As there was no measure of work-study boundary congruence suitable for use with university students, and there was little consensus over the conceptualisation and operationalisation of the work-study boundary congruence construct, the main aims of this research program were to (a) develop and validate a psychometrically sound measure of work-study boundary congruence for university students who work, and (b) apply the scale to assess mediation and moderation models of work-study boundary congruence. This research was guided by boundary management, person-environment fit (Kreiner, Hollensbe, & Sheep, 2009), and conservation of resources theories (Hobfoll, 1988; Hobfoll, Freedy, Lane, & Geller, 1990), so as to increase our understanding of how students manage the dual, competing roles of work and study. This PhD thesis reports a series of three studies presented as three journal articles, which meet these objectives: Study 1 addresses the first aim; with Studies 2 and 3 fulfilling the second aim. Study 1 involved conducting focus groups, generating and reviewing items, and piloting and testing a Work-Study Congruence Scale suitable for use with working students. A 16-item scale, with four key dimensions of work-study boundary congruence with university demands/resources, occupation, family, and leisure was devised. Data from a large sample of Australian university students (N = 511; 70% female; Mage = 24.02 years) were collected and analysed for item suitability and factorial structure (exploratory and confirmatory analyses). Scale reliability and validity were assessed. Study 2 used the newly developed scale to examine potential mechanisms (i.e., work-study facilitation and work-study conflict) underpinning the associations between work-study boundary congruence and the general well-being and engagement of university students. This study employed a sample of Australian university students (N = 251; 70% female; Mage = 24.68 years). The results showed that work-study boundary congruence was related to more work-study facilitation and less work-study conflict. Conflict was associated with less well-being, while facilitation was associated more with well-being and greater university engagement. Work-study boundary congruence was associated positively with well-being via both work-study facilitation and work-study conflict. Study 3 used a sample of Australian university students (N = 401; 67% female; Mage = 21.71 years) and investigated the mediating effect of work-study boundary congruence on the associations between contextual supports (of family and work) and well-being, effort at university, and perceived future employability. The study also assessed the moderating role of proactive personality in these associations. The results showed that contextual supports were associated with well-being, academic performance, and perceived employability via work-study boundary congruence, and that lower levels of proactivity strengthened the associations between both contextual supports and work-study boundary congruence, which, in turn, strengthened the indirect links between the contextual supports and outcomes via work-study boundary congruence. The development and validation of a new work-study boundary congruence scale suitable for use with university students, which is theory-driven, will contribute to the boundary management research and literature. University students, and those who work to assist them, will benefit from understanding the effect of work-study boundary congruence on student well-being, university engagement, academic performance, and perceived employability. In addition, they will also benefit from understanding the roles that contextual supports and personal resources play in boundary management, especially where there are risks of working students experiencing poor well-being and/or discontinuing with their studies at university due to an inability to manage conflicting life roles. Increasing research in this area will also inform training and intervention programs that can help students manage their multiple roles.Thesis (PhD Doctorate)
Doctor of Philosophy (PhD)
School of Applied Psychology
Griffith Health
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Arthur, David Benjamin. "Extracellular nucleotide signaling in neuronal differentiation and survival Multiple roles of the P2Y₂ receptor /." Connect to a 24 p. preview or request complete full text in PDF format. Access restricted to UC campuses, 2006. http://wwwlib.umi.com/cr/ucsd/fullcit?p3205808.
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Thesis (Ph. D.)--University of California, San Diego, 2006.Title from first page of PDF file (viewed April 3, 2006). Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 139-159).
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McCormick, Michael Craig. "The roles of EXT1 and EXT2 in heparan sulfate polymerization and hereditary multiple exostoses." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2000. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0019/NQ48673.pdf.
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Parker, Jodey Alexander. "The Caenorhabditis elegans homologue of huntingtin interacting protein 1 has multiple roles in development." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 2001. http://www.collectionscanada.ca/obj/s4/f2/dsk3/ftp04/NQ61154.pdf.
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Joliat, Melissa J. "Autoimmunity, Immune Deficiency and Cancer: Multiple Roles of the Protein Tyrosine Phosphate SHP-1." Fogler Library, University of Maine, 2001. http://www.library.umaine.edu/theses/pdf/JoliatMJ2001.pdf.
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Sin, Yuk-ling, and 冼玉玲. "The stress of multiple roles: the case of part-time learners and their coping." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 1994. http://hub.hku.hk/bib/B31977765.
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O'Toole, Alison. "Tumour Necrosis Factor-#alpha# signalling : potential roles in the pathophysiology of multiple organ failure." Thesis, University of Bristol, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364934.
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Hockley, James Robert Frederick. "Multiple roles for NaV1.9 in visceral afferent activation by noxious mechanical and inflammatory stimuli." Thesis, Queen Mary, University of London, 2014. http://qmro.qmul.ac.uk/xmlui/handle/123456789/9105.
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Chronic visceral pain affects millions of individuals worldwide, remains poorly understood, and current therapeutics are constrained by undesirable adverse events. Inflammation and distension of visceral organs are common causes of pain, suggesting drugs targeting these signalling pathways may be efficacious visceral treatments. The voltage-gated sodium channel subtype 1.9 (NaV1.9) has been strongly associated with the development of inflammatory pain by rodent studies and more recently, by the identification of channelopathies in man. The aim of these studies was to investigate the role of NaV1.9 in visceral afferent signalling in the gut. Data from this thesis demonstrates that NaV1.9 is expressed by approximately half of gut-projecting rodent dorsal root ganglia sensory neurons. Consistent with significant expression in visceral afferents, NaV1.9 is required for normal mechanosensation, and for the direct excitation and mechanical hypersensitisation of mouse colonic afferents by inflammatory mediators applied as an inflammatory soup (bradykinin, ATP, histamine, PGE2, and 5HT) or derived from man (as inflammatory bowel disease tissue supernatants). Additionally, the importance of P2Y receptor activation in both rodent and human gut to algogenic purinergic signalling was demonstrated. Collectively, these results demonstrate that NaV1.9, is required for persistence of responses to intense mechanical stimulation, contributes to inflammatory mechanical hypersensitivity, and is essential for activation by noxious inflammatory mediators, including those from diseased human bowel. These findings suggest that NaV1.9 represents a high-value target for development of visceral analgesics.
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Ross, A. J. "Multiple roles of integrin-α3 in the development and maintenance of the neuromuscular junction." Thesis, University College London (University of London), 2015. http://discovery.ucl.ac.uk/1471753/.
Full textAbstract:
The neuromuscular junction (NMJ) is the synaptic contact between motoneurons and muscle, where neurotransmission results in the contraction of the muscle fibres. The basal lamina instructs NMJ development at virtually every stage. Largely, the functions of the basal lamina are mediated through interactions with cell surface adhesion receptors; however, less is known about the identities and roles of these at the NMJ. Integrin-α3 is an extracellular matrix receptor that has previously been identified at the NMJ active zones, the sites of neurotransmitter release in the presynaptic terminus. As integrin-α3 binds to laminin-α4 and other active zone components, my hypothesis is that it may be important for relaying signals provided by the basal lamina during NMJ development. In this study, the integrin-α3 knockout mouse was used to explore the functions of this protein at the NMJ. Mutants displayed defects in active zone assembly and developmental motoneuron patterning. NMJs frequently resembled those found in aged animals, and in some cases, nerve terminals were detached from the synaptic cleft. Finally, electrophysiological analysis revealed defects in neurotransmission at mutant NMJs, including reduced efficiency of synaptic vesicle release, and impaired sensitivity of nerve terminals to external Ca2+. Previous studies have implicated integrin-α3 in muscle development; however, I find no expression of integrin-α3 in the muscle, and no defects in myogenesis in integrin-α3 mutants. These results indicate multiple roles for integrin-α3 at the NMJ, for active zone assembly, adhesion of nerve terminal, morphological integrity and neurotransmission. To my knowledge, this study identifies for the first time a cell surface receptor for the anchorage of pre- and postsynaptic elements at the NMJ. These results suggest that alterations in integrin-α3 expression or function may underlie some of the changes associated with ageing at the NMJ, and that mutations in its encoding gene may cause myasthenic syndromes.
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Takara, Thomas J. (Thomas Joji). "Multiple roles of the replication initiation protein Cdtl during helicase loading in S. cerevisiae." Thesis, Massachusetts Institute of Technology, 2011. http://hdl.handle.net/1721.1/65299.
Full textAbstract:
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Biology, 2011.Cataloged from PDF version of thesis.
Includes bibliographical references.
The faithful transmission of genetic information is critical for the events of cell division and propagation. In eukaryotic cells, chromosomal replication is carefully coordinated with the cell cycle to ensure that the entire genome is replicated exactly once prior to cell division. Underpinning this coordination is the tightly regulated loading and activation of the eukaryotic replicative DNA helicase, the hetero-hexameric Mcm2-7 complex. As cells enter G1 phase of the cell cycle, all potential sites of replication initiation are selected by the loading of inactive Mcm2-7 double hexamers. The anti-parallel orientation of the Mcm2-7 hexamers within the double hexamer is proposed to be critical to establish bidirectional sister replisomes upon helicase activation in S phase. Although the proteins involved in helicase loading are known, the mechanism that drives Mcm2-7 double-hexamer formation and loading is unclear. The replication initiation protein Cdtl is essential for loading Mcm2-7 onto origin DNA, but its functions during the loading event are unclear. Through analysis of Cdtl mutations, I identified regions of Cdtl that are essential for Mcm2-7 helicase binding, origin recruitment, and activation. I found that multiple Cdtl molecules are recruited to the origin during the helicase-loading process, and disrupting of the assembly of the multi-Cdtl intermediate prevents Mcm2-7 loading. Finally, I demonstrated that the Nterminal domain of Cdtl, although dispensable for stable helicase loading, is required for subsequent helicase activation and replication initiation. These findings reveal that Cdtl performs multiple functions during helicase loading and influences the competence of loaded Mcm2-7 to subsequently become activated. This work provides insight into the process of Mcm2-7 double-hexamer loading and supports a model in which Cdtl initiates Mcm2-7 double-hexamer formation early in the helicase-loading process.
by Thomas J. Takara.
Ph.D.