Relevant bibliographies by topics / Multilocus DNA analyses

Academic literature on the topic 'Multilocus DNA analyses'

Author: Grafiati
Published: 10 December 2022
Last updated: 29 January 2023

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles

Select a source type:

Contents

Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Multilocus DNA analyses.'

Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.

You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.

Journal articles on the topic "Multilocus DNA analyses"

1

Koehler, Andreas, Helge Karch, Thomas Beikler, Thomas F. Flemmig, Sebastian Suerbaum, and Herbert Schmidt. "Multilocus sequence analysis of Porphyromonas gingivalis indicates frequent recombination." Microbiology 149, no. 9 (September 1, 2003): 2407–15. http://dx.doi.org/10.1099/mic.0.26267-0.

Full text
Abstract:
In this study, the genetic relationship of 19 Porphyromonas gingivalis isolates from patients with periodontitis was investigated by multilocus sequence analysis. Internal 400–600 bp DNA fragments of the 10 chromosomal genes ef-tu, ftsQ, hagB, gpdxJ, pepO, mcmA, dnaK, recA, pga and nah were amplified by PCR and sequenced. No two isolates were identical at all 10 loci. Phylogenetic analyses indicated a panmictic population structure of P. gingivalis. Split decomposition analysis, calculation of homoplasy ratios and analyses of clustered polymorphisms all indicate that recombination plays a major role in creating the genetic heterogeneity of P. gingivalis. A standardized index of association of 0·0898 indicates that the P. gingivalis genes analysed are close to linkage equilibrium.
APA, Harvard, Vancouver, ISO, and other styles
2

Murphy, Nicholas P., Rachael A. King, and Steven Delean. "Species, ESUs or populations? Delimiting and describing morphologically cryptic diversity in Australian desert spring amphipods." Invertebrate Systematics 29, no. 5 (2015): 457. http://dx.doi.org/10.1071/is14036.

Full text
Abstract:
Cryptic species are frequently being discovered in refugial habitats, such as desert springs and groundwater systems. Unfortunately, many of these taxa remain as unnamed entities years after their initial discovery. Recent advances in the use of molecular data and coalescent analyses allow DNA-based delimitation of species to move from single locus, tree-based methods to multilocus coalescent analyses. This study compares two DNA-based approaches to delimit species of putatively cryptic freshwater amphipods (Chiltoniidae) from desert springs in central Australia. In addition, a morphometric analysis of 11 characters was undertaken to determine whether the DNA-delimited species were morphologically distinguishable. The single locus method results in identification of lineages that are not supported as species under the multilocus coalescent analyses. We conclude that Wangiannachiltonia guzikae King, 2009, as currently circumscribed, represents six genetically distinct amphipod species, and we describe and name these species despite no clear diagnosable morphological differences. Critically, all of these newly recognised species have extremely limited distributions, which increases the biodiversity significance of their desert spring habitat.
APA, Harvard, Vancouver, ISO, and other styles
3

Zeng, Nian-Kai, Li-Ping Tang, Yan-Chun Li, Bau Tolgor, Xue-Tai Zhu, Qi Zhao, and Zhu L. Yang. "The genus Phylloporus (Boletaceae, Boletales) from China: morphological and multilocus DNA sequence analyses." Fungal Diversity 58, no. 1 (July 29, 2012): 73–101. http://dx.doi.org/10.1007/s13225-012-0184-7.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

O'Donnell, Kerry, Stacy Sink, María Mercedes Scandiani, Alicia Luque, Analía Colletto, Marisa Biasoli, Lisandro Lenzi, et al. "Soybean Sudden Death Syndrome Species Diversity Within North and South America Revealed by Multilocus Genotyping." Phytopathology® 100, no. 1 (January 2010): 58–71. http://dx.doi.org/10.1094/phyto-100-1-0058.

Full text
Abstract:
Sudden death syndrome (SDS) of soybean has become a serious constraint to the production of this crop in North and South America. Phenotypic and multilocus molecular phylogenetic analyses, as well as pathogenicity experiments, have demonstrated that four morphologically and phylogenetically distinct fusaria can induce soybean SDS. Published molecular diagnostic assays for the detection and identification of these pathogens have reported these pathogens as F. solani, F. solani f. sp. glycines, or F. solani f. sp. phaseoli, primarily because the species limits of these four pathogens were only recently resolved. In light of the recent discovery that soybean SDS and Phaseolus and mung bean root rot (BRR) are caused by four and two distinct species, respectively, multilocus DNA sequence analyses were conducted to assess whether any of the published molecular diagnostic assays were species-specific. Comparative DNA sequence analyses of the soybean SDS and BRR pathogens revealed that highly conserved regions of three loci were used in the design of these assays, and therefore none were species-specific based on our current understanding of species limits within the SDS–BRR clade. Prompted by this finding, we developed a high-throughput multilocus genotyping (MLGT) assay which accurately differentiated the soybean SDS and two closely related Phaseolus and mung BRR pathogens based on nucleotide polymorphism within the nuclear ribosomal intergenic spacer region rDNA and two anonymous intergenic regions designated locus 51 and 96. The single-well diagnostic assay, employing flow cytometry and a novel fluorescent microsphere array, was validated by independent multilocus molecular phylogenetic analysis of a 65 isolate design panel. The MLGT assay was used to reproducibly type a total of 262 soybean SDS and 9 BRR pathogens. The validated MLGT array provides a unique molecular diagnostic for the accurate identification and molecular surveillance of these economically important plant pathogens.
APA, Harvard, Vancouver, ISO, and other styles
5

Huang, Chien-Hsun, Chih-Chieh Chen, Jong-Shian Liou, Ai-Yun Lee, Jochen Blom, Yu-Chun Lin, Lina Huang, and Koichi Watanabe. "Genome-based reclassification of Lactobacillus casei: emended classification and description of the species Lactobacillus zeae." International Journal of Systematic and Evolutionary Microbiology 70, no. 6 (June 1, 2020): 3755–62. http://dx.doi.org/10.1099/ijsem.0.003969.

Full text
Abstract:
Taxonomic relationships between Lactobacillus casei , Lactobacillus paracasei and Lactobacillus zeae have long been debated. Results of previous analyses have shown that overall genome relatedness indices (such as average nucleotide identity and core nucleotide identity) between the type strains L. casei ATCC 393T and L. zeae ATCC 15820T were 94.6 and 95.3 %, respectively, which are borderline for species definition. However, the digital DNA‒DNA hybridization value was 57.3 %, which was clearly lower than the species delineation threshold of 70 %, and hence raised the possibility that L. casei could be reclassified into two species. To re-evaluate the taxonomic relationship of these taxa, multilocus sequence analysis (MLSA) based on the concatenated five housekeeping gene (dnaJ, dnaK, mutL, pheS and yycH) sequences, phylogenomic and core genome multilocus sequence typing analyses, gene presence and absence profiles using pan-genome analysis, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling analysis, cellular fatty acid compositions, and phenotype analysis were carried out. The results of phenotypic characterization, MLSA, whole-genome sequence-based analyses and MALDI-TOF MS profiling justified an independent species designation for the L. zeae strains, and supported an emended the description of the name of Lactobacillus zeae (ex Kuznetsov 1956) Dicks et al. 1996, with ATCC 15820T (=DSM 20178T=BCRC 17942T) as the type strain.
APA, Harvard, Vancouver, ISO, and other styles
6

Fang, Wei, Min-wei Guo, Zhi-yong Ruan, Han Xue, Lai-fa Wang, Guo-zhong Tian, Chun-gen Piao, and Yong Li. "Multilocus sequence analysis of the genus Kurthia, and a description of Kurthia populi sp. nov." International Journal of Systematic and Evolutionary Microbiology 65, Pt_11 (November 1, 2015): 3788–93. http://dx.doi.org/10.1099/ijsem.0.000494.

Full text
Abstract:
Four novel bacterial strains belonging to the genus Kurthia were isolated from the surface of a weevil of the family Curculionidae (strain 10y-14T), and from bark samples of hybrid poplar, Populus × euramericana (strains 6-3, 2-5 and 06C10-3-14), in Puyang, Henan Province, China. Phylogenetic analyses of the 16S rRNA gene and multilocus sequence analysis (MLSA) data showed that the four strains form a distinct cluster in the genus Kurthia, indicating that they all belong to a single taxon within the genus. DNA–DNA hybridization levels between strain 10y-4T and Kurthia huakuii LAM0618T and Kurthia massiliensis DSM 24639T were 58.31 and 53.92 %, respectively. This indicates that the four novel strains represent a species distinct from these two closely related species. The DNA G+C content of the novel strains was 42.1–42.6 %. The major fatty acids were iso-C15 : 0 and anteiso-C15 : 0.The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid and unidentified aminophospholipids. The predominant menaquinones were MK-7 (90 %) and MK-6 (10 %). The major cell-wall amino acids were lysine, alanine, glutamic acid and glycine. On the basis of the MLSA and 16S rRNA gene sequence phylogenetic analyses, DNA–DNA reassociation values, DNA base composition, and biochemical and phenotypic characteristics, the four strains are considered to represent a novel species within the genus Kurthia, for which the name Kurthia populi sp. nov. is proposed. The type strain is 10y-14T ( = CFCC 11600T = KCTC 33522T).
APA, Harvard, Vancouver, ISO, and other styles
7

Thompson, Cristiane C., Vanessa E. Emmel, Erica L. Fonseca, Michel A. Marin, and Ana Carolina P. Vicente. "Streptococcal taxonomy based on genome sequence analyses." F1000Research 2 (March 1, 2013): 67. http://dx.doi.org/10.12688/f1000research.2-67.v1.

Full text
Abstract:
The identification of the clinically relevant viridans streptococci group, at species level, is still problematic. The aim of this study was to extract taxonomic information from the complete genome sequences of 67 streptococci, comprising 19 species, by means of genomic analyses, multilocus sequence analysis (MLSA), average amino acid identity (AAI), genomic signatures, genome-to-genome distances (GGD) and codon usage bias. We then attempted to determine the usefulness of these genomic tools for species identification in streptococci. Our results showed that MLSA, AAI and GGD analyses are robust markers to identify streptococci at the species level, for instance,S. pneumoniae,S. mitis, andS. oralis. AStreptococcusspecies can be defined as a group of strains that share ≥ 95% DNA similarity in MLSA and AAI, and > 70% DNA identity in GGD. This approach allows an advanced understanding of bacterial diversity.
APA, Harvard, Vancouver, ISO, and other styles
8

GUPTA, BHAVNA, NALINI SRIVASTAVA, and APARUP DAS. "Inferring the evolutionary history of IndianPlasmodium vivaxfrom population genetic analyses of multilocus nuclear DNA fragments." Molecular Ecology 21, no. 7 (February 21, 2012): 1597–616. http://dx.doi.org/10.1111/j.1365-294x.2012.05480.x.

Full text
APA, Harvard, Vancouver, ISO, and other styles
9

Zarza, Eugenia, Elizabeth M. Connors, James M. Maley, Whitney L. E. Tsai, Peter Heimes, Moises Kaplan, and John E. McCormack. "Combining ultraconserved elements and mtDNA data to uncover lineage diversity in a Mexican highland frog (Sarcohyla; Hylidae)." PeerJ 6 (December 11, 2018): e6045. http://dx.doi.org/10.7717/peerj.6045.

Full text
Abstract:
Molecular studies have uncovered significant diversity in the Mexican Highlands, leading to the description of many new endemic species. DNA approaches to this kind of species discovery have included both mitochondrial DNA (mtDNA) sequencing and multilocus genomic methods. While these marker types have often been pitted against one another, there are benefits to deploying them together, as linked mtDNA data can provide the bridge between uncovering lineages through rigorous multilocus genomic analysis and identifying lineages through comparison to existing mtDNA databases. Here, we apply one class of multilocus genomic marker, ultraconserved elements (UCEs), and linked mtDNA data to a species complex of frogs (Sarcohyla bistincta, Hylidae) found in the Mexican Highlands. We generated data from 1,891 UCEs, which contained 1,742 informative SNPs for S. bistincta and closely related species and captured mitochondrial genomes for most samples. Genetic analyses based on both whole loci and SNPs agree there are six to seven distinct lineages within what is currently described as S. bistincta. Phylogenies from UCEs and mtDNA mostly agreed in their topologies, and the few differences suggested a more complex evolutionary history of the mtDNA marker. Our study demonstrates that the Mexican Highlands still hold substantial undescribed diversity, making their conservation a particularly urgent goal. The Trans-Mexican Volcanic Range stands out as a significant geographic feature in Sarcohyla and may have acted as a dispersal corridor for S. bistincta to spread to the north. Combining multilocus genomic data with linked mtDNA data is a useful approach for identifying potential new species and associating them with already described taxa, which will be especially important in groups with undescribed subadult phenotypes and cryptic species.
APA, Harvard, Vancouver, ISO, and other styles
10

Mammella, Marco A., Frank N. Martin, Santa O. Cacciola, Michael D. Coffey, Roberto Faedda, and Leonardo Schena. "Analyses of the Population Structure in a Global Collection of Phytophthora nicotianae Isolates Inferred from Mitochondrial and Nuclear DNA Sequences." Phytopathology® 103, no. 6 (June 2013): 610–22. http://dx.doi.org/10.1094/phyto-10-12-0263-r.

Full text
Abstract:
Genetic variation within the heterothallic cosmopolitan plant pathogen Phytophthora nicotianae was determined in 96 isolates from a wide range of hosts and geographic locations by characterizing four mitochondrial (10% of the genome) and three nuclear loci. In all, 52 single-nucleotide polymorphisms (SNPs) (an average of 1 every 58 bp) and 313 sites with gaps representing 5,450 bases enabled the identification of 50 different multilocus mitochondrial haplotypes. Similarly, 24 SNPs (an average of 1 every 69 bp), with heterozygosity observed at each locus, were observed in three nuclear regions (hyp, scp, and β-tub) differentiating 40 multilocus nuclear genotypes. Both mitochondrial and nuclear markers revealed a high level of dispersal of isolates and an inconsistent geographic structuring of populations. However, a specific association was observed for host of origin and genetic grouping with both nuclear and mitochondrial sequences. In particular, the majority of citrus isolates from Italy, California, Florida, Syria, Albania, and the Philippines clustered in the same mitochondrial group and shared at least one nuclear allele. A similar association was also observed for isolates recovered from Nicotiana and Solanum spp. The present study suggests an important role of nursery populations in increasing genetic recombination within the species and the existence of extensive phenomena of migration of isolates that have been likely spread worldwide with infected plant material.
APA, Harvard, Vancouver, ISO, and other styles
More sources

Dissertations / Theses on the topic "Multilocus DNA analyses"

1

Scholes, Derek Trevor. "Analysis of relatedness within a moorland population of the red grouse (Lagopus lagopus scoticus) by multilocus DNA fingerprinting." Thesis, University of Liverpool, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283067.

Full text
APA, Harvard, Vancouver, ISO, and other styles

Books on the topic "Multilocus DNA analyses"

1

Threlfall, E. J., J. Wain, and C. Lane. Salmonellosis. Oxford University Press, 2011. http://dx.doi.org/10.1093/med/9780198570028.003.0030.

Full text
Abstract:
Salmonellosis remains the second most common form of bacterial food-poisoning in the UK and in most of the developed economies. Although the number of isolations per annum has declined since 2000, over 10,000 laboratory-confirmed cases are recognised each year in England and Wales, and over 150,000 in Europe. Most of infections are associated with contaminated food, particularly of poultry origin, but also may originate from cattle and pigs, and to a lesser extent, sheep. The most common serovars from cases of human infection is Enteritidis, followed by Typhimurium. Contact with pets, particularly reptiles and amphibians is becoming an increasing problem and infections can be severe, particularly in children. Accurate and reproducible methods of identification and subtyping are crucial for meaningful epidemiological investigations, and traditional phenotypic methods of typing are now being supplemented by DNA- based methods such as pulsed-field gel electrophoresis, variable number of tandem repeats analysis, and multilocus sequence typing. The use of such methods in combination with phenotypic methods has been invaluable for outbreak control at the international level. The occurrence of resistance to antimicrobial drugs is an increasing problem, particularly in relation to the development of resistance to antimicrobials regarded as ‘critically-important’ for last resort therapy in humans. Control measures such as vaccination of poultry flocks appear to have had a substantial impact on the number of infections with Salmonella Enteritidis. Nevertheless good hygiene practices in both catering establishments and the home remain essential for the control of infections at the local level.
APA, Harvard, Vancouver, ISO, and other styles

Book chapters on the topic "Multilocus DNA analyses"

1

Krawczak, M., and B. Bockel. "The formal analysis of multilocus DNA fingerprints." In DNA Fingerprinting: State of the Science, 249–55. Basel: Birkhäuser Basel, 1993. http://dx.doi.org/10.1007/978-3-0348-8583-6_21.

Full text
APA, Harvard, Vancouver, ISO, and other styles
2

Schneider, Peter M., Rolf Fimmers, Ulrike Schacker, and Christian Rittner. "Paternity Analysis Using the Multilocus DNA Probe MZ 1.3." In Advances in Forensic Haemogenetics, 179–81. Berlin, Heidelberg: Springer Berlin Heidelberg, 1992. http://dx.doi.org/10.1007/978-3-642-77324-2_52.

Full text
APA, Harvard, Vancouver, ISO, and other styles
3

Epplen, Jörg T., and Judith MÁthÉ. "Multilocus DNA Fingerprinting Using Nonradioactively Labeled Oligonucleotide Probes Specific for Simple Repeat Elements." In Nonradioactive Analysis of Biomolecules, 468–74. Berlin, Heidelberg: Springer Berlin Heidelberg, 2000. http://dx.doi.org/10.1007/978-3-642-57206-7_40.

Full text
APA, Harvard, Vancouver, ISO, and other styles
4

Stacey, Glyn N., Bryan J. Bolton, and Alan Doyle. "Multilocus Dna Fingerprinting in the Analysis of Cell Stocks: Stability Studies and Application to a Wide Range of Species." In Animal Cell Technology: Basic & Applied Aspects, 31–39. Dordrecht: Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2844-5_5.

Full text
APA, Harvard, Vancouver, ISO, and other styles
5

Hillel, Jossi, E. Ann Dunnington, Alon Haberfeld, Uri Lavi, Avigdor Cahaner, Orit Gal, Yoram Plotsky, H. L. Marks, and Paul B. Siegel. "Multilocus DNA Markers: Applications in Poultry Breeding and Genetic Analyses." In Manipulation of the Avian Genome, 243–56. CRC Press, 2019. http://dx.doi.org/10.1201/9780203748282-16.

Full text
APA, Harvard, Vancouver, ISO, and other styles
6

"Probes, Allele Mutations, and Restriction Enzymes." In DNA Fingerprinting, edited by Lorne t. Kirby. Oxford University Press, 1993. http://dx.doi.org/10.1093/oso/9780716770015.003.0010.

Full text
Abstract:
Positive identification is the ultimate objective of forensic analysis of blood and other tissue specimens. Nucleotide probes can be very effective tools for detecting genetic markers in this identification process. The genetic markers should be highly polymorphic; allelic variants should be easily and readily detectable; if amplification is required, the alleles should be efficiently amplified using PCR technology; and a statistically sound estimate of the population allele and genotype frequencies should be available. Probes are single-stranded fragments of DNA or RNA containing the complementary code for a specific sequence of genome bases. Probes available for DNA profile analysis will, no doubt, eventually number in the hundreds. Currently, the most valuable detect tandem repetitive sequence fragments either at a specific locus under high-stringency analysis conditions or at numerous loci under low-stringency conditions. Each locus consists of many possible alleles with frequencies that vary depending on the specific population. Other factors also enter into the selection of probes, including ease of amplification, stability, cross-reactivity, and general availability. Rate of allele mutation is also a prime consideration in probe selection. Mutation can be considered at two levels: as the basis for the large number of tandem repeat (VNTR) alleles formed during evolution and as a possible reason for spurious unassignable bands in typing analysis. Although highly unlikely, somatic mutations may be of concern in forensic testing if DNA from different tissues, such as blood and hair roots, are being matched. Germ line (gamete) mutations must be considered when parentage analyses are undertaken. These situations could give rise to false negative results and, therefore, false exclusions. Different considerations also apply for single versus multilocus probes. If a band that is not seen in the putative father is detected in an offspring, the man could incorrectly be excluded if the single-locus probe approach is used. This situation would necessitate testing with more than the usual four or five probes.
APA, Harvard, Vancouver, ISO, and other styles
7

"Propagated Fish in Resource Management." In Propagated Fish in Resource Management, edited by ANNE P. HENDERSON, ADRIAN P. SPIDLE, and TIM L. KING. American Fisheries Society, 2004. http://dx.doi.org/10.47886/9781888569698.ch51.

Full text
Abstract:
<em>Abstract.</em>—Captive Atlantic sturgeon <em>Acipenser oxyrinchus </em>considered for use as broodstock in a restoration program were genotyped using nuclear DNA microsatellites and compared to wild collections from the Hudson River, New York (source of parents of the captive sturgeon) and from Albemarle Sound, North Carolina. Because the potential broodfish were the progeny of a small number of parents, maintaining genetic diversity and minimizing inbreeding is essential to a successful breeding and supplementation program. The microsatellite loci used in this analysis generated unique multilocus genotypes for each of 136 Atlantic sturgeon. Analyses indicated significant genetic separation between the New York and North Carolina collections and correctly identified the potential broodstock as a subset of the Hudson River population. Pairwise genetic distance (–ln proportion of shared alleles) between half and full siblings in the potential broodfish was as great as 1.386, a value exceeded by only 36% of the sampled broodfish pairs available for mating. Because the current broodstock population does not seem to have deviated far from their ancestral population in the Hudson River, progeny from that broodstock, or the parents themselves, would seem to be genetically suitable for release back into the Hudson River.
APA, Harvard, Vancouver, ISO, and other styles
8

Allendorf, Fred W., W. Chris Funk, Sally N. Aitken, Margaret Byrne, and Gordon Luikart. "Genetic Identification." In Conservation and the Genomics of Populations, 512–39. Oxford University Press, 2022. http://dx.doi.org/10.1093/oso/9780198856566.003.0022.

Full text
Abstract:
Genetic analysis allows genetic identification of individuals, populations, and species for a range of conservation purposes, including wildlife trafficking, detecting invasive species, determining relatedness in captive breeding, and identifying community composition. Genomics provides increased power for genetic identification at individual, population, and species levels, and is a key tool in wildlife forensics. DNA barcoding using specific markers has become common for species identification, and metabarcoding of environmental or mixed samples through genomics informs community composition, diet analysis, and identifying cryptic, elusive, or rare individuals and species. Genetic identification has become prominent in wildlife forensics providing critical evidence to enable prosecutions and deter illegal wildlife activities. Multilocus genotyping allows determination of parentage and relatedness, population assignment, and origin of samples. Determination of the relatedness or parentage of individuals provides information on identification of dispersal and migration patterns, and facilitates management of captive breeding populations.
APA, Harvard, Vancouver, ISO, and other styles
9

"DMA Amplification." In DNA Fingerprinting, edited by Lorne t. Kirby. Oxford University Press, 1993. http://dx.doi.org/10.1093/oso/9780716770015.003.0008.

Full text
Abstract:
Amplification of DNA may be necessary to increase the quantity of sample available for profiling, to reduce the analysis time, or to produce probes for the hybridization process (Higuchi 1989, Li 1988, Marx 1988, Mullis 1990, Paabo 1989, Saiki 1986). Stretches of nucleotides up to at least 3,000 bp from any DNA-containing samples may be efficiently amplified by the polymerase chain reaction (PCR). Alternatively, living tissue can be placed in culture, and fibroblasts, epithelial type cells, or lymphoblasts grown. The culture process differs considerably from the PCR approach in that the total genome is reproduced. Also, tissue culture is usually at least a two-week procedure, whereas the polymerase chain reaction requires only a few hours. Cultured cells can be used for enzyme and other biochemical tests, and storage in liquid nitrogen is a standard practice for regrowth at a later time. Probe material, that is, DNA capable of hybridizing with its complementary region in the genome, must be amplified, aliquoted, and stored to provide an ongoing source for use with each profile analysis. Probe amplification has been mainly carried out in bacterial culture; however, probes can be chemically synthesized as discussed in Chapter 2 or amplified by the PCR system. At least 10 to 50 ng of high molecular weight genomic DNA are required for VNTR analysis using single-locus probes, and at least 0.5 to 1.0 μg required if multilocus probes are used. If only a small quantity of DNA is available, amplification using the PCR may be the only feasible option for obtaining sufficient material for analysis. PCR has revolutionized the approach to the recovery of DNA from a variety of sources. Microgram quantities of DNA can be produced in vitro by the amplification of picogram starting amounts. Single-copy genomic sequences greater than 2 kb in length have been amplified more than 10 millionfold in a few hours. Amplified material can also be directly sequenced without the necessity of incorporating DNA fragments into vectors such as M13 (Gyllensten 1989,1989a). Availability of oligonucleotide primers is the key to the amplification process.
APA, Harvard, Vancouver, ISO, and other styles
You might also be interested in the extended bibliographies on the topic 'Multilocus DNA analyses' for particular source types:
Journal articles
We offer discounts on all premium plans for authors whose works are included in thematic literature selections. Contact us to get a unique promo code!

To the bibliography