Relevant bibliographies by topics / Multi-photon excitation microscopy

Academic literature on the topic 'Multi-photon excitation microscopy'

Author: Grafiati
Published: 6 September 2023

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Journal articles on the topic "Multi-photon excitation microscopy"

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Piston, David W. "Multi-Photon Excitation Microscopy: An Old Idea in Quantum Theory Applied to Modern Scientific Problems." Microscopy and Microanalysis 6, S2 (August 2000): 1180–81. http://dx.doi.org/10.1017/s1431927600038393.

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Multi-photon excitation microscopy provides attractive advantages over confocal microscopy for three-dimensionalry resolved fluorescence imaging and photochemistry. The most commonly used type of multi-photon excitation is two-photon excitation where simultaneous absorption of two photons leads to a single quantitized event. The powerful advantages of using two-photon excitation microscopy arise from the basic physical principle that the absorption depends on the square of the excitation intensity. In practice, two-photon excitation is generated by focusing a single pulsed laser through the microscope. As the laser beam is focused, the photons become more crowded, but the only place at which they are crowded enough to generate an appreciable amount of two-photon excitation is at the focus. Above and below the focus, the photon density is not high enough for two of them to interact with a single fluorophore at the same time. This dramatic difference between confocal and two-photon excitation microscopy is shown in Fig. 1.
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ROTHSTEIN, EMILY C., MICHAEL NAUMAN, SCOTT CHESNICK, and ROBERT S. BALABAN. "Multi-photon excitation microscopy in intact animals." Journal of Microscopy 222, no. 1 (April 2006): 58–64. http://dx.doi.org/10.1111/j.1365-2818.2006.01570.x.

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Masters, Barry R., and Peter T. C. So. "Multi-photon Excitation Microscopy and Confocal Microscopy Imaging of In Vivo Human Skin: A Comparison." Microscopy and Microanalysis 5, no. 4 (July 1999): 282–89. http://dx.doi.org/10.1017/s1431927699990311.

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Abstract: We compare here multi-photon excitation microscopy and tandem scanning reflected light confocal microscopy for the microscopic observation of human skin in vivo. Multi-photon excitation is induced by a 80-MHz pulse train of femtosecond laser pulses at 780 nm wavelength. This nonlinear microscopic technique is inherently suitable for tissue fluorescence imaging because of its deeper penetration depth and lower specimen photodamage. This technique has noninvasively obtained tissue structural information in human epidermis and dermis. Alternatively, tandem scanning confocal light microscopy based on a white light source can provide video-rate image acquisition with high resolution and high contrast. Reflected light confocal methods have been used to obtain images from the skin surface to the epidermal–dermal junction. The relative merits of these two techniques can be identified by comparing three-dimensionally resolved images obtained from the forearm skin of the same volunteer.
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Cheng, Ping-chin, Chi-Kuang Sun, Fu-Jen Kao, and Bai-Ling Lin. "Non-linear Spectral Microscopy-Multi-Photon Fl, SHG and THG." Microscopy and Microanalysis 7, S2 (August 2001): 1026–27. http://dx.doi.org/10.1017/s1431927600031202.

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The non-linear nature of multi-photon fluorescence (FL) excitation, SHG and THG restricts the signal detecting volume to the vicinity of the focal point. As a result, the technology has intrinsic optical sectioning capability. The use of multi-photon fluorescence excitation also allows micro-fluorometry at high spatial resolution. Figure 1 shows a conventional optical micrograph of maize protoplasts, the time lapse fluorescence spectral change from a single chloroplast is shown in FIG 2. Under high intensity illumination, biological specimen not only emits fluorescence, but also generates harmonic emissions. in addition to the Ti-sapphire laser commonly used in multiphoton microscopy, the use of ultra-fast Cr-fosterite laser made simultaneous detecting two- and three-photon fluorescence, SHG and THG possible. in addition to the fluorescence signals generated by multi-photon excitation process, non-linear phenomena such as harmonic generation can also provide useful information about the structure and optical properties of a specimen (Kao et al., 2000). Simultaneous recording the spectral response in an image (x-y-λ) can provide insight about the nature of the signal.
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Kao, F. J., B. L. Lin, and P. C. Cheng. "Multi-photon Fluorescence Micro-spectroscopy of Plant Tissues." Microscopy and Microanalysis 6, S2 (August 2000): 808–9. http://dx.doi.org/10.1017/s1431927600036539.

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Considering its non-linear nature, two-photon excitation may generate very different spectral response in samples when compared with single photon excitation. It is thus necessary to measure the two-photon spectra of samples, so that the two-photon fluorescence microscopic images can be properly interpreted. Fluorescence spectra obtained from bulk samples may not provide useful information for microscopy. For instance, due to the relatively small contribution to the total fluorescence intensity, a small number of fluorescent particles in a generally fluorescing specimen may escape detection when the spectrum of the specimen as a whole is obtained. Under two-photon excitation, the background noise can be greatly reduced due to the naturally limited excitation volume of focused laser beam. In addition, signals resulted from second harmonic generation (SHG) may be mixed with low level broad-band background autofluorescence which is commonly found in biological specimen. Therefore, measuring fluorescence spectrum from a micro-focused volume is essential for the proper interpretation of multi-photon fluorescence images.
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Guo, Yong, Hongyi Han, Luwei Wang, Yinru Zhu, Xinwei Gao, Zhigang Yang, Xiaoyu Weng, Wei Yan, and Junle Qu. "Label free deep penetration single photon microscopic imaging with ultralong anti-diffracting beam." Applied Physics Letters 121, no. 2 (July 11, 2022): 023701. http://dx.doi.org/10.1063/5.0097959.

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Label free single photon microscopic imaging has natural advantages in noninvasive in vivo tissue imaging such as high resolution and rapid imaging speed. Although label free multi-photon microscopy can be used for imaging thick tissue samples, it requires high excitation light power and is phototoxic to the samples. Conventional label free single photon microscopy requires lower excitation light power, but it has limited imaging depth. Observing some highly scattering thick tissue samples with single photon microscopy is a great challenge. To solve the problem, we developed a label free deep penetration single photon microscopic imaging technique with an ultralong anti-diffracting (UAD) beam. The penetrating ability of the UAD beam was verified by passing through turbid media and performed with autofluorescence of chloroplasts in fresh Epipremnum aureum leaves. Benefiting from the anti-diffracting properties and the elongated focal depth of the UAD beam, single photon UAD microscopy has deeper penetration depth and better anti-scattering ability and is one of the ideal methods to observe the deep structure of biological samples.
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Cheng, P. C., B. L. Lin, F. J. Kao, C. K. Sun, and I. Johnson. "Multi-Photon Fluorescence Spectroum of Common Nucleic Acid Probes." Microscopy and Microanalysis 6, S2 (August 2000): 820–21. http://dx.doi.org/10.1017/s143192760003659x.

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Fluorescent probes are commonly used in biological fluorescence microscopy for tracking specific structures and sub-cellular compartments, and for indicating cellular ionic conditions. Recent development in multi-photon fluorescence microscopy has greatly expanded the usage of fluorescent probes in biomedical research. Considering its non-linear nature, two-photon excitation may generate very different fluorescence spectral response in the sample when compared with single photon excitation. It is thus necessary to measure the two-photon spectra of various fluorescent probes, so that two-photon fluorescence microscopy may be operated effectively and the images properly interpreted. This report represents the first installment of a continued effort in characterizing the multi-photon fluorescence spectra of commonly used bio-probes.Two-photon fluorescence spectra excited with near infrared at 780nm were obtained with a SpectraPro-500 spectrophotometer (Acton Research) equipped with a TE-cooled PMT and coupled to a Spectra-Physics Tsunami Ti-sapphire laser pumped by a Coherent Verdi solid-state laser operated at 85MHz, l00fs pulse.
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Mertz, J. "Molecular photodynamics involved in multi-photon excitation fluorescence microscopy." European Physical Journal D - Atomic, Molecular and Optical Physics 3, no. 1 (August 1, 1998): 53–66. http://dx.doi.org/10.1007/s100530050148.

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Schweitzer, Andreas, Heinz Eipel, and Christoph Cremer. "Rapid image acquisition in multi-photon excitation fluorescence microscopy." Optik 115, no. 3 (2004): 115–20. http://dx.doi.org/10.1078/0030-4026-00339.

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Goswami, Debabrata, Dhiman Das, and Soumendra Nath Bandyopadhyay. "Resolution enhancement through microscopic spatiotemporal control." Faraday Discussions 177 (2015): 203–12. http://dx.doi.org/10.1039/c4fd00177j.

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Operating at biologically benign conditions, multi-photon fluorescence imaging microscopy has benefitted immensely from recent developments in microscopic resolution enhancement. Fluorescence microscopy continues to be the best choice for experiments on live specimens, however, multi-photon fluorescence imaging often suffers from overlapping fluorescence of typical dyes used in microscopy, limiting its scope. This limitation has been the focus of our research where we show that by making simple modifications to the laser pulse structure, it is possible to resolve these overlapping fluorescence complications. Specifically, by using pairs of femtosecond pulses with variable delay in place of single pulse excitation, we show controlled fluorescence excitation or suppression of one of the fluorophores over the other through wave-packet interferometry. Such an effect prevails even after the fluorophore coherence timescale, which effectively results in a higher spatial resolution. Here we extend the effect of our pulse-pair technique to microscopic axial resolution experiments and show that such pairs of pulses can also ‘enhance’ axial resolution.
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Dissertations / Theses on the topic "Multi-photon excitation microscopy"

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Tsikritsis, Dimitrios. "Vibrational spectroscopy and microscopy in colorectal cancer." Thesis, University of Edinburgh, 2018. http://hdl.handle.net/1842/33049.

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This project set out to examine the possibility that by acquiring Raman spectra and performing multi-photon imaging we can get better diagnosis and understanding of the biochemistry of an individual cancerous tumour and distinguish it from the healthy tissue. Within the frame of this study, colorectal primary and secondary cancer cells are examined with Raman spectroscopy in order to (i) study and distinguish them according to their chemical composition by applying multivariate methods and (ii) determine whether Raman spectroscopy can identify the cells which are the link between primary and secondary colorectal cancer cells, the so-called Cancer Stem Cells. The second part of this thesis is based on tissue studies. Human colorectal tissue sections are examined in a label-free manner with the use of multi-photon imaging modes (i) Two photon excitation fluorescence, (ii) stimulated Raman scattering and (iii) second harmonic generation, in order to determine whether these can provide fast and accurate diagnosis of colorectal cancer. These techniques were able to distinguish between healthy and cancerous tissue regions, based on the chemically-specific images of the tissue microenvironment and architecture. The hypothesis of Cancer stem cell is examined with the use of Raman spectroscopy shown that the CSCs have some small differences according to their tissue origin.
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Freeman, Kim Renee. "In situ three-dimensional reconstruction of mouse heart sympathetic innervation by two-photon excitation fluorescence imaging." Thesis, 2014. http://hdl.handle.net/1805/4030.

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Indiana University-Purdue University Indianapolis (IUPUI)
The sympathetic nervous system strongly modulates the contractile and electrical function of the heart. The anatomical underpinnings that enable a spatially and temporally coordinated dissemination of sympathetic signals within the cardiac tissue are only incompletely characterized. In this work we took the first step of unraveling the in situ 3D microarchitecture of the cardiac sympathetic nervous system. Using a combination of two-photon excitation fluorescence microscopy and computer-assisted image analyses, we reconstructed the sympathetic network in a portion of the left ventricular epicardium from adult transgenic mice expressing a fluorescent reporter protein in all peripheral sympathetic neurons. The reconstruction revealed several organizational principles of the local sympathetic tree that synergize to enable a coordinated and efficient signal transfer to the target tissue. First, synaptic boutons are aligned with high density along much of axon-cell contacts. Second, axon segments are oriented parallel to the main, i.e., longitudinal, axes of their apposed cardiomyocytes, optimizing the frequency of transmitter release sites per axon/per cardiomyocyte. Third, the local network was partitioned into branched and/or looped sub-trees which extended both radially and tangentially through the image volume. Fourth, sub-trees arrange to not much overlap, giving rise to multiple annexed innervation domains of variable complexity and configuration. The sympathetic network in the epicardial border zone of a chronic myocardial infarction was observed to undergo substantive remodeling, which included almost complete loss of fibers at depths >10 µm from the surface, spatially heterogeneous gain of axons, irregularly shaped synaptic boutons, and formation of axonal plexuses composed of nested loops of variable length. In conclusion, we provide, to the best of our knowledge, the first in situ 3D reconstruction of the local cardiac sympathetic network in normal and injured mammalian myocardium. Mapping the sympathetic network connectivity will aid in elucidating its role in sympathetic signal transmisson and processing.
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Book chapters on the topic "Multi-photon excitation microscopy"

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So, Peter T. C. "Multi-photon Excitation Fluorescence Microscopy." In Frontiers in Biomedical Engineering, 529–44. Boston, MA: Springer US, 2003. http://dx.doi.org/10.1007/978-1-4419-8967-3_35.

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Denk, Winfried, David W. Piston, and Watt W. Webb. "Multi-Photon Molecular Excitation in Laser-Scanning Microscopy." In Handbook Of Biological Confocal Microscopy, 535–49. Boston, MA: Springer US, 2006. http://dx.doi.org/10.1007/978-0-387-45524-2_28.

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Gu, Min. "Three-Dimensional Localisation of Fluorescence Resonance Energy Transfer in Living Cells under Two-Photon Excitation." In Multi-Modality Microscopy, 246–56. WORLD SCIENTIFIC, 2006. http://dx.doi.org/10.1142/9789812774620_0013.

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Conference papers on the topic "Multi-photon excitation microscopy"

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Webb, Watt W., and Chris Xu. "Multi-photon Molecular Excitation to Illuminate Non-linear Laser Microscopy." In International Conference on Ultrafast Phenomena. Washington, D.C.: Optica Publishing Group, 1996. http://dx.doi.org/10.1364/up.1996.wb.1.

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Molecular excitation by two or more infra red photons simultaneously absorbed from strongly focused 100 femtosecond mode locked laser pulses provides intrinsic 3-d resolution for fluorescence microscopy and photochemical micro pharmacology in living biological cells. Confinement of non-linear excitation to the focal volume illuminated by ~1016 photons/nm2s eliminates out of focus photobleaching and photodamage and provides intrinsic 3-d resolution. To facilitate this technology new methods have developed for accurate measurements of multi-photon excitation cross sections and spectra in the absence of adequate data to guide non-linear laser microscopy applications. Absolute cross section measurements have been facilitated by development of a method that takes advantage of the interference of excitation pulse trains that are time shifted by a conventional Michaelson interferometer to allow variable relative delay of two spatially superimposed beams. Two photon excitation with single mode CW lasers provides an independent absolute calibration of cross sections. Measurements of more than twenty useful two-photon excitation spectra (690-1050nm) have revealed some with large blue shifts of the excitation peak. Two photon excitation spectra of some asymmetric molecules superimpose on the one photon absorption spectra (with wavelength doubled) presumably due to relaxation of parity selection rules in these cases. Most of the tested fluorescent molecules show blue shifted excitation peaks with strongly enhanced cross sections exceeding those observed at twice the one photon absorption. No red shifts of the excitation spectra have been detected. Three photon excitation spectra tend to mimic the one photon absorption spectra. Multi- photon excited emission spectra appear to duplicate the one photon fluorescence emission spectra.
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Kubo, Toshiki, Kenta Temma, Kazunori Sugiura, Hajime Shinoda, Kai Lu, Nicholas I. Smith, Tomoki Matsuda, Takeharu Nagai, and Katsumasa Fujita. "Multi-photon activation of fluorescent proteins using visible wavelength for high-resolution imaging." In Conference on Lasers and Electro-Optics/Pacific Rim. Washington, D.C.: Optica Publishing Group, 2022. http://dx.doi.org/10.1364/cleopr.2022.p_cm15_05.

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We experimentally demonstrated the photo-activation of reversibly photo-switchable fluorescent proteins by visible-wavelength two-photon excitation and applied the activation technique in confocal imaging of biological cells. Higher spatial resolution than conventional confocal microscopy was confirmed.
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Wang, Ke, Tzu-Ming Liu, Juwell Wu, Nicholas G. Horton, Charles P. Lin, and Chris Xu. "Multi-color femtosecond source for simultaneous excitation of multiple fluorescent proteins in two-photon fluorescence microscopy." In SPIE BiOS, edited by Ammasi Periasamy, Karsten König, and Peter T. C. So. SPIE, 2013. http://dx.doi.org/10.1117/12.2000583.

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Eibl, Matthias, Sebastian Karpf, Hubertus Hakert, Daniel Weng, Torben Blomker, and Robert Huber. "Pulse-to-pulse wavelength switchable fiber laser for multi-color two-photon excitation fluorescence (TPEF) microscopy." In 2017 Conference on Lasers and Electro-Optics Europe (CLEO/Europe) & European Quantum Electronics Conference (EQEC). IEEE, 2017. http://dx.doi.org/10.1109/cleoe-eqec.2017.8086485.

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Qing Li, Xiaobo Zhou, Zhigang Deng, Matthew Baron, Merilee A. Teylan, Yong Kim, and Stephen T. C. Wong. "A novel surface-based geometric approach for 3D dendritic spine detection from multi-photon excitation microscopy images." In 2009 IEEE International Symposium on Biomedical Imaging: From Nano to Macro (ISBI). IEEE, 2009. http://dx.doi.org/10.1109/isbi.2009.5193290.

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Bonora, Stefano, Sujin Lee, Yifan Jian, Michelle Cua, Edward N. Pugh, Robert J. Zawadzki, and Marinko V. Sarunic. "Multi-actuator adaptive lens for wavefront correction in optical coherence tomography and two-photon excitation fluorescence microscopy (Conference Presentation)." In Adaptive Optics and Wavefront Control for Biological Systems II, edited by Thomas G. Bifano, Sylvain Gigan, and Joel Kubby. SPIE, 2016. http://dx.doi.org/10.1117/12.2211810.

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Zeng, Shaoqun, Qingming Luo, Wei Zhang, Qian Liu, Chengjun Li, and Qiang Lu. "In vivo functional microscopic imaging based on multi-photon excitation: principles and methods." In Biomedical Topical Meeting. Washington, D.C.: OSA, 2002. http://dx.doi.org/10.1364/bio.2002.sud36.

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