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Academic literature on the topic 'MSPSHA'

Author: Grafiati

Published: 1 April 2023

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Journal articles on the topic "MSPSHA"

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Han, Sushan, Junzo Norimine, and Brown C. Wendy. "Rapid deletion of antigen-specific CD4+ T cells following infection represents a strategy of immune evasion and persistence for Anaplasma marginale (133.36)." Journal of Immunology 182, no. 1_Supplement (April 1, 2009): 133.36. http://dx.doi.org/10.4049/jimmunol.182.supp.133.36.

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Abstract We reported the rapid and permanent deletion of immunization-induced bovine CD4 T cell responses to Anaplasma marginale major surface proteins (MSPs) after infection. To determine if antigen-specific T cells were anergic, sequestered, or deleted, CD4 T cells specific for a MSP1a T cell epitope were enumerated with a DRB3*1101 tetramer. The pre-challenge frequency of tetramer+ CD4+ cells of 0.025% dropped rapidly at the peak of infection to control levels of 0.0003%, paralleling loss of antigen specific T cell responses. Background frequencies of tetramer+ CD4+ T cells in spleen and lymph node indicated lack of sequestration. We hypothesize that this rapid and long-term deletion of specific T cells is driven by high antigen load during acute and persistent infection. To test this, cattle immunized with MSP1a and MSP2 of A. marginale will be challenged with A. marginale or A. centrale, a subspecies in which MSP2 but not MSP1a epitopes are conserved with those in A. marginale. We predict deletion of CD4+ T cells specific for MSP1a and MSP2 after infection with A. marginale, but only deletion of MSP2-specific T cells after infection with A. centrale, as MSP1a-specific T cells will not be exposed to high antigen load in the latter cattle. This experiment will determine if infection-mediated CD4+ T cell depletion is dependent on the presence of specific antigen in the infecting pathogen.

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Kesmen, H., A. K. Cengiz, A. Bilgici, G. Alayli, and D. Durmus. "FRI0616-HPR SOCIAL SUPPORT WORKS BETTER IN RHEUMATOID ARTHRITIS THAN FIBROMYALGIA." Annals of the Rheumatic Diseases 79, Suppl 1 (June 2020): 913.3–913. http://dx.doi.org/10.1136/annrheumdis-2020-eular.5673.

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Background:Social support is defined as a helpful resource that can meet an individual’s urgent needs and is provided by a network of others, such as family members, friends, colleagues and other communities. Perceived social support (PSS) is the subjective support that individuals may experience, emphasizing the individual’s self-understanding, experience and feelings of social support from different sources. Social support directly exerts beneficial effects on the health-related quality of life in rheumatoid arthritis and fibromyalgia patients.Objectives:The aim of this study is to compare the effect of perceived social support on quality of life of rheumatoid arthritis and fibromyalgia patients. The effect of social support from family, friends and significant others are examined separately.Methods:Fifty-eight patients with rheumatoid arthritis, fifty patients with fibromyalgia and fifty healhy controls were enrolled in the study. Short form 36 (SF-36) was used to determine the quality of life and Multidimensional Perceived Social Support Scale (MSPSS) was used to determine the percieved social support. The effect of three factors in MSPSS (family, friends, significant others) on quality of life of rheumatoid arthritis and fibromyalgia patients were examined seperately.Results:Fibromyalgia patients had lower scores than the rheumatoid arthritis patients and healthy controls regarding the physical function, pain, social functioning and mental health subscales of SF-36 (p<0.05). Regarding the all MSPSS scores, there was no significant difference between the three groups (p>0.05). In rheumatoid arthritis patients, MSPSS-friend and MSPSS-significant others scores were positively correlated with all subscales of SF-36. MSPSS-family score was only correlated with pain, social functioning and mental health subscales of SF-36. But in fibromyalgia patients none of the three MSPSS scores had significant correlation with SF-36 subscales (Table-1).Conclusion:Perceived social support especially from friends and significant others, has an important positive effect on the quality of life of rheumatoid arthritis patients. For fibromyalgia patients the effect of perceived social support on quality of life is lower than that is seen in rheumatoid arthritis.References:[1] De Maria M, Vellone E, Durante A, Biagioli V, Matarese M. Psychometric evaluation of the Multidimensional Scale of Perceived Social Support (MSPSS) in people with chronic diseases. Ann Ist Super Sanita 2018; 54(4):308-315.Table 1.The effect of perceived social support on quality of life in fibromyalgia and rheumatoid arthritis patients (FMS: Fibromyalgia, RA: Rheumatoid arthritis, *p<0.05 **p<0.01)SF-36Physical functioningSF-36PainSF-36General healthSF-36Social functioningSF-36MentalhealthFMS-MSPSSr-0.0340.015-0.187-0.121-0.115Familyp0.8160.9150.1940.4010.428FMS-MSPSSr0.1540.1920.2210.1820.089Friendp0.2850.1810.1230.2060.539FMS-MSPSSr0.1630.2480.1190.175-0.004Significant othersp0.2580.0820.4120.2250.976RA-MSPSSr0,1810,2900,2250,4480,340Familyp0,1730,027 *0,0890,001**0,009**RA-MSPSSr0,3570,3520,3330,3760,389Friendp0,006*0,007**0,011*0,004**0,003**RA-MSPSSr0,3100,3100,3290,3150,463Significant othersp0,018*0,018**0,012*0,016*0,001*RA-MSPSSr0,3340,3660,3500,4320,487Totalp0,010*0,005**0,007**0,001**0,001**FMS-MSPSSr0.1440.2510.0930.1530.024Totalp0.3200.2790.5190.2900.867Disclosure of Interests:None declared

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Barbet, A. F., R. Blentlinger, Jooyoung Yi, A. M. Lundgren, E. F. Blouin, and K. M. Kocan. "Comparison of Surface Proteins of Anaplasma marginale Grown in Tick Cell Culture, Tick Salivary Glands, and Cattle." Infection and Immunity 67, no. 1 (January 1, 1999): 102–7. http://dx.doi.org/10.1128/iai.67.1.102-107.1999.

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ABSTRACT Anaplasma marginale, a tick-borne rickettsial pathogen of cattle, infects bovine erythrocytes, resulting in mild to severe hemolytic disease that causes economic losses in domestic livestock worldwide. Recently, the Virginia isolate of A. marginalewas propagated in a continuous tick cell line, IDE8, derived from embryonic Ixodes scapularis. Development of A. marginale in cell culture was morphologically similar to that described previously in ticks. In order to evaluate the potential of the cell culture-derived organisms for use in future research or as an antigen for serologic tests and vaccines, the extent of structural conservation of the major surface proteins (MSPs) between the cell culture-derived A. marginale and the bovine erythrocytic stage, currently the source of A. marginale antigen, was determined. Structural conservation on the tick salivary-gland stage was also examined. Monoclonal and monospecific antisera against MSPs 1 through 5, initially characterized against erythrocyte stages, also reacted with A. marginale from cell culture and tick salivary glands. MSP1a among geographic A. marginaleisolates is variable in size because of different numbers of a tandemly repeated 28- or 29-amino-acid peptide. The cell culture-derivedA. marginale maintained the same-size MSP1a as that found on the Virginia isolate of A. marginale in bovine erythrocytes and tick salivary glands. Although differences were observed in the polymorphic MSP2 antigen between culture and salivary-gland stages, MSP2 did not appear to vary, by two-dimensional gel electrophoresis, during continuous passage in culture. These data show that MSPs of erythrocyte-stage A. marginale are present on culture stages and may be structurally conserved during continuous culture. The presence of all current candidate diagnostic and vaccine antigens suggests that in vitro cultures are a valuable source of rickettsiae for basic research and for the development of improved diagnostic reagents and vaccines against anaplasmosis.

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Lee, Sung-Hoon, Kack-Kyun Kim, and Bong-Kyu Choi. "Upregulation of Intercellular Adhesion Molecule 1 and Proinflammatory Cytokines by the Major Surface Proteins of Treponema maltophilum and Treponema lecithinolyticum, the Phylogenetic Group IV Oral Spirochetes Associated with Periodontitis and Endodontic Infections." Infection and Immunity 73, no. 1 (January 2005): 268–76. http://dx.doi.org/10.1128/iai.73.1.268-276.2005.

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ABSTRACT Treponema maltophilum and Treponema lecithinolyticum belong to the group IV oral spirochetes and are associated with endodontic infections, as well as periodontitis. Recently, the genes encoding the major surface proteins (Msps) of these bacteria (MspA and MspTL, respectively) were cloned and sequenced. The amino acid sequences of these proteins showed significant similarity. In this study we analyzed the functional role of these homologous proteins in human monocytic THP-1 cells and primary cultured periodontal ligament (PDL) cells using recombinant proteins. The complete genes encoding MspA and MspTL without the signal sequence were cloned into Escherichia coli by using the expression vector pQE-30. Fusion proteins tagged with N-terminal hexahistidine (recombinant MspA [rMspA] and rMspTL) were obtained, and any possible contamination of the recombinant proteins with E. coli endotoxin was removed by using polymyxin B-agarose. Flow cytometry showed that rMspA and rMspTL upregulated the expression of intercellular adhesion molecule 1 (ICAM-1) in both THP-1 and PDL cells. Expression of proinflammatory cytokines, such as interleukin-6 (IL-6) and IL-8, was also induced significantly in both cell types by the Msps, as determined by reverse transcription-PCR and an enzyme-linked immunosorbent assay, whereas IL-1β synthesis could be detected only in the THP-1 cells. The upregulation of ICAM-1, IL-6, and IL-8 was completely inhibited by pretreating the cells with an NF-κB activation inhibitor, l-1-tosylamido-2-phenylethyl chloromethyl ketone. This suggests involvement of NF-κB activation. The increased ICAM-1 and IL-8 expression in the THP-1 cells obtained with rMsps was not inhibited in the presence of the IL-1 receptor antagonist (IL-1ra), a natural inhibitor of IL-1. Our results show that the Msps of the group IV oral spirochetes may play an important role in amplifying the local immune response by continuous inflammatory cell recruitment and retention at an infection site by stimulation of expression of ICAM-1 and proinflammatory cytokines.

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Graham, Alister W. "Black hole and nuclear cluster scaling relations: Mbh ∝ Mnc2.7±0.7." Proceedings of the International Astronomical Union 10, S312 (August 2014): 269–73. http://dx.doi.org/10.1017/s1743921315008017.

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AbstractThere is a growing array of supermassive black hole and nuclear star cluster scaling relations with their host spheroid, including a bent (black hole mass)–(host spheroid mass) Mbh–Msph relation and a different (massive compact object mass)–(host spheroid velocity dispersion) Mmco–σ relations for black holes and nuclear star clusters. By combining the observed Mbh ∝ σ5.5 relation with the observed Mnc ∝ σ1.6–2.7 relation, we derive the expression Mbh ∝ Mnc2–3.4, which should hold until the nuclear star clusters are eventually destroyed in the larger core-Sérsic spheroids. This new mass scaling relation helps better quantify the rapid evolutionary growth of massive black holes in dense star clusters, and the relation is consistently recovered when coupling the observed Mnc ∝ Msph0.6–1.0 relation with the recently observed quadratic relation Mbh ∝ Msph2 for Sérsic spheroids.

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Gao, Yangjun, and Guangyun Li. "Two Regional Deployment Algorithms of Distributed GNSS Forwarding Spoofer for Multiple Receiver Sensors." Sensors 22, no. 20 (October 14, 2022): 7793. http://dx.doi.org/10.3390/s22207793.

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Global navigation satellite system (GNSS) spoofing technology is an effective way to protect sensitive facilities and control foreign objects. To realize effective distributed GNSS forwarding spoofing for multiple receiver sensors in the area, the following research work is carried out: first, the GNSS forwarding spoofing model is established, including a forwarding spoofing mathematical model and an asynchronous traction spoofing method; second, the function scope of forwarding spoofing is fully evaluated; third, two forwarding spoofer area deployment algorithms for multi-spoofer multi-target (MSMT) are proposed—the multi-target same-point spoofing algorithm (MSPSA) is suitable for the same-point spoofing of multiple receiver sensors, and the multi-target different-point spoofing algorithm (MDPSA) is suitable for the different-point spoofing of multiple receiver sensors; and four, the experimental tests of MSPSA and MDPSA for MSMT were carried out. The experimental results show that MSPSA and MDPSA can respectively give the most appropriate deployment scheme of spoofing according to the various needs of the spoofer. For example, the number of spoofing devices is 1, the total distance between each spoofer and each receiver is 40,345.1 m, and the critical spoofing rate (CSR) and spoofing success rate (SSR) are both 100%. The performance of the spoofing deployment scheme can meet the needs of the spoofer.

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Garcia-Garcia, Jose C., José de la Fuente, Gianna Bell-Eunice, Edmour F. Blouin, and Katherine M. Kocan. "Glycosylation of Anaplasma marginale Major Surface Protein 1a and Its Putative Role in Adhesion to Tick Cells." Infection and Immunity 72, no. 5 (May 2004): 3022–30. http://dx.doi.org/10.1128/iai.72.5.3022-3030.2004.

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ABSTRACT Anaplasma marginale, the causative agent of bovine anaplasmosis, is a tick-borne rickettsial pathogen of cattle that multiplies in erythrocytes and tick cells. Major surface protein 1a (MSP1a) and MSP1b form the MSP1 complex of A. marginale, which is involved in adhesion of the pathogen to host cells. In this study we tested the hypothesis that MSP1a and MSP1b were glycosylated, because the observed molecular weights of both proteins were greater than the deduced molecular masses. We further hypothesized that the glycosylation of MSP1a plays a role in adhesion of A. marginale to tick cells. Native and Escherichia coli-derived recombinant MSP1a and MSP1b proteins were shown by gas chromatography to be glycosylated and to contain neutral sugars. Glycosylation of MSP1a appeared to be mainly O-linked to Ser/Thr residues in the N-terminal repeated peptides. Glycosylation may play a role in adhesion of A. marginale to tick cells because chemical deglycosylation of MSP1a significantly reduced its adhesive properties. Although the MSP1a polypeptide backbone alone was adherent to tick cell extract, the glycans in the N-terminal repeats appeared to enhance binding and may cooperatively interact with one or more surface molecules on host cells. These results demonstrated that MSP1a and MSP1b are glycosylated and suggest that the glycosylation of MSP1a plays a role in the adhesion of A. marginale to tick cells.

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Arulkanthan, Appudurai, Wendy C. Brown, Travis C. McGuire, and Donald P. Knowles. "Biased Immunoglobulin G1 Isotype Responses Induced in Cattle with DNA Expressing msp1a of Anaplasma marginale." Infection and Immunity 67, no. 7 (July 1, 1999): 3481–87. http://dx.doi.org/10.1128/iai.67.7.3481-3487.1999.

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ABSTRACT Immunization with the native major surface protein 1 (MSP1) (a heterodimer containing disulfide and noncovalently bonded polypeptides designated MSP1a and MSP1b) of the erythrocytic stage ofAnaplasma marginale conferred protection against homologous challenge (G. H. Palmer, A. F. Barbet, W. C. Davis, and T. C. McGuire, Science 231:1299–1302, 1986). The MSP1a polypeptide possesses a conserved neutralization-sensitive epitope. In the present study, the immune response to DNA-mediated immunization using msp1a was studied. The plasmid pVCL/MSP1a, which encodes the complete msp1a gene of A. marginaleunder the control of human cytomegalovirus immediate-early enhancer/promoter and intron A, was constructed. The immune responses elicited by immunization with pVCL/MSP1a into cardiotoxin-induced regenerating muscle were evaluated in mice and cattle. Antibody reactive with native MSP1a was detected in pooled sera of immunized BALB/c mice 3 weeks following primary immunization. Two calves seronegative for A. marginale were immunized four times, at weeks 0, 3, 7, and 13, with pVCL/MSP1a. By 8 weeks, both calves responded to MSP1a with an antibody titer of 1:100, which peaked at 1:1,600 and 1:800 by 16 weeks after the initial immunization. Interestingly, immunoblotting with anti-immunoglobulin G1 (anti-IgG1) and anti-IgG2 specific monoclonal antibodies revealed a restricted IgG1 anti-MSP1a response in both animals. T-lymphocyte lines, established after the fourth immunization, proliferated specifically againstA. marginale homogenate and purified MSP1 in a dose-dependent manner. These data provide a basis for an immunization strategy to direct bovine immune responses by using DNA vaccine vectors containing single or multiple genes encoding major surface proteins ofA. marginale.

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Porter, Ben, Claire A. Kolaja, Teresa M. Powell, Jacqueline C. Pflieger, Valerie A. Stander, and Richard F. Armenta. "Reducing the Length of the Multidimensional Scale of Perceived Social Support." European Journal of Psychological Assessment 36, no. 5 (September 2020): 829–38. http://dx.doi.org/10.1027/1015-5759/a000553.

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Abstract. The Multidimensional Scale of Perceived Social Support (MSPSS) is a widely used 12-item measure that assesses perceived social support from three sources: friends, family, and significant others. Previously published psychometric properties indicate that a shorter version of this scale may adequately assess perceived social support and reduce participant burden. The current studies sought to develop such a reduced scale across two studies. Study 1 examined a sample of spouses of US military personnel ( N = 5,436) randomly separated into exploratory and confirmatory samples. In the exploratory sample, we developed a 6-item reduced MSPSS using multidimensional item response theory. In the confirmatory sample, the reduced MSPSS fit the hypothesized structure and was highly correlated with the full MSPSS. Study 2 administered the full and reduced MSPSS separately within a sample of undergraduate students ( N = 188). The reduced MSPSS had high correlations with the full measure ( r = .90) and fit the hypothesized factor structure. Across both studies, correlations with related constructs were similar between the reduced and full MSPSS, demonstrating almost no loss of construct validity. Overall, the reduced MSPSS captured perceived social support with little loss of information. This reduced scale may be useful for minimizing survey length and participant burden.

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Islam, Nurul. "Psychometric properties of the Bangla version of multidimensional scale of perceived social support." Psihologija, no. 00 (2020): 26. http://dx.doi.org/10.2298/psi200319026i.

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The Bangla version of the Multidimensional Scale of Perceived Social Support (MSPSS-B) is a popular psychological assessment tool in Bangladesh. It has largely been used to measure perceived social support of Bangladeshi people. In spite of its popularity, it had not gone through an extensive validation procedure yet. Even its psychometric properties were not tested before, except for the test-retest reliability. This cross-sectional study aimed to examine the psychometric properties of MSPSS-B through a questionnaire survey among 812adult Bangladeshi people. The MSPSS-B revealed a three-factor structure through exploratory factor analysis (EFA) on the first split sample (n = 403), explaining 71.64% of the total variance. Acceptable goodness of fit indices (?2/df = 4.293, p = .000, GFI = .920, CFI = .926, TLI = .904, SRMR = .063, and RMSEA = .078) in the MSPSS-B were obtained through confirmatory factor analysis (CFA) on the second split sample (n = 409). The three-factor structure of the MSPSS-B was the same as the original English MSPSS. Acceptable internal item consistencies, significant test-retest reliabilities, reliabilities between two scale versions, convergent and discriminant validities, and measurement invariance between two gender groups were also established in the MSPSS-B through different statistical analyses. Thus, the MSPSS-B with its three factors can be used as a valid and reliable measure to assess the perceived social support of Bangladeshi people.

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Dissertations / Theses on the topic "MSPSHA"

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Prater, Gary L. "NPSAT1 Missile System Prelaunch Safety Package (MSPSP)." Thesis, Monterey, Calif. : Springfield, Va. : Naval Postgraduate School ; Available from National Technical Information Service, 2004. http://library.nps.navy.mil/uhtbin/hyperion/04Jun%5FPrater.pdf.

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Arlery, Fabien. "Formes d’ondes MSPSR, traitements et performances associés." Thesis, Evry, Institut national des télécommunications, 2017. http://www.theses.fr/2017TELE0005/document.

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Aujourd’hui, les systèmes MSPSR (Multi-Static Primary Surveillance Radar) passifs se sont installés de manière durable dans le paysage de la surveillance aérienne [1]. L’intérêt que suscitent ces nouveaux systèmes provient du fait qu’en comparaison aux radars mono-statiques utilisés actuellement, les systèmes MSPSR reposent sur une distribution spatiale d’émetteurs et de récepteurs offrant des avantages en termes de fiabilité (redondance), de coûts (absence de joints tournants et émetteurs moins puissants) et de performances (diversité spatiale). Toutefois, le défaut majeur du MSPSR passif réside en l’absence de formes d’ondes dédiées due à l’exploitation d’émetteurs d’opportunités tels que les émetteurs de radio FM (Frequency Modulation) et/ou de DVB-T (Digital Video Broadcasting-Terrestrial) [2]. Afin de pallier à ce défaut, il est envisagé d’utiliser des émetteurs dédiés permettant l’emploi de formes d’ondes optimisées pour une application radar, on parle alors de MSPSR actif. Cette thèse se place dans ce cadre et a pour objectif d’étudier et de définir la ou les formes d’ondes ainsi que les traitements associés permettant d’atteindre de meilleurs performances : une meilleure flexibilité sur la disposition du système (positionnement des émetteurs libres), une continuité de service (non dépendance d’un système tiers) et de meilleurs performances radars (e.g. en terme de précision des mesures, détections, …). Dans ce but, cette thèse étudie : - Les critères de sélection des codes : comportement des fonctions d’ambiguïtés, PAPR (Peak to Average Power Ratio), efficacité spectrale, etc... ; - Les formes d’ondes utilisées en télécommunication (scrambling code, OFDM) afin d’identifier leur possible réemploi pour une application radar ; - L’utilisation d’algorithmes cycliques pour générer des familles de séquences adaptées à notre problème ; - Une approche basée sur une descente de gradient afin de générer des familles de codes de manière plus efficiente ; - Et l’évaluation des performances de ces différents algorithmes à travers l’établissement d’une borne supérieure sur le niveau maximum des lobes secondaires et à travers le dépouillement des données enregistrées suite à des campagnes d’essais
Nowadays, MSPSR (Multi-Static Primary Surveillance Radar) systems are sustainably settled in air surveillance program [1]. Compared to mono-static radar currently in use, an MSPSR system is based on a sparse network of transmitters (Tx) and receivers (Rx) interconnected to a Central Unit and offers advantages in terms of reliability, cost and performance.Two kinds of MSPSR systems exist: the Passive form and the Active one. While the Passive MSPSR uses transmitters of opportunity such as radio Frequency Modulation (FM) transmitters and/or Digital Video Broadcasting-Terrestrial (DVB-T) transmitters [2], the Active MSPSR uses dedicated transmitters, which emit a waveform that is controlled and designed for a radar application. Each receiver processes the signal coming from all transmitters and reflected on the targets; and the Central Unit restores the target location by intersecting “ellipsoids” from all (transmitter, receiver) pairs. Compared to passive MSPSR, the main advantages of the active MSPSR are the use of dedicated waveforms that allow reaching better performances (like a better association of the transmitters’ contributions at the receiver level); more flexibility in the deployment of transmitters and receivers station (in order to meet the requirements in localisation accuracy and in horizontal and altitude coverages); and the guarantee of having a service continuity. On this purpose, this thesis analyses the differents codes criteria such as the ambiguity function behaviour, the PAPR (Peak to Average Power Ratio), the spectrum efficiency, etc... . Then, in order to find dedicated waveforms for MSPSR systems, one solution is to find easily-constructed families of sequences. Thus building on the works carried out by the Telecommunication field for solving multi-user issues, this document investigates the application of spreading codes and OFDM signals in MSPSR concept. Besides, another solution is to directly generate a set of sequences. Based on cyclic algorithms in [3] we derive a new algorithm that allows to optimize sets of sequences. Similarly, using a gradient descent approach, we develop a more efficient algorithm than the cyclic one. Finally, in order to evaluate the performances of the different algorithms, this thesis generalizes the Levenshtein Bound, establishes new lower bounds on the PSLR (Peak Sidelobe Level Ratio) in mismatched filter case, and studies real data recorded during some trials

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Santos, Paula de Souza. "Epitopos imunodominantes da MSP1a de Anaplasma marginale e suas aplicações diagnósticas e vacinais." Universidade Federal de Uberlândia, 2011. https://repositorio.ufu.br/handle/123456789/15730.

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Anaplasmosis, a persistent intraerythrocytic infection of cattle by Anaplasma marginale, causes severe anemia and a higher rate of abortion, resulting in significant loss to both dairy and beef industries. Clinical diagnosis is based on symptoms and confirmatory laboratory tests are required. Currently, all the diagnostic assays have been developed with whole antigens with indirect ELISA based on multiple epitopes. In a pioneer investigation we demonstrated the use of critical motifs of an epitope as biomarkers for immunosensor applications. Mimotopes of the MSP1a protein functional epitope were obtained through Phage Display after three cycles of selection of a 12-mer random peptide library against the neutralizing monoclonal antibody 15D2. Thirty-nine clones were randomly selected, sequenced, translated and aligned with the native sequence. The consensus sequences SxSSQSEASTSSQLGA was obtained, which is located in C-terminal end of the 28-aa repetitive motif of the MSP1a protein, but the alignment and sequences variation among mimotopes allowed us to map the critical motif STSSxL within the consensus sequence. Based on these results, two peptides were chemically synthesized; one based on the critical motif (STSSQL, Am1) and the other based on the consensus sequence aligned with the native epitope (SEASTSSQLGA, Am2). Sera from 24 infected and 52 healthy animals were tested by ELISA for reactivity against Am1 and Am2, which presented sensitivities of 96% and 100%, respectively. The Am1 peptide was incorporated onto a biolectrode (graphite modified with poly-3-hydroxyphenylacetic acid) and direct serum detection was demonstrated by impedance, differential pulse voltammetry, and atomic force microscopy. The electrochemical sensor system proved to be highly effective in discriminating sera from positive and negative animals. These immunosensors were highly sensitive and selective for positive IgG, contaminants did not affect measurements, and were based on a simple, fast and reproducible electrochemical system.
Anaplasmose, uma infecção intraeritrocitária obrigatória de bovinos, causada pela Anaplasma marginale, causa severa anemia e alta taxa de aborto, resultando em perda significativa para a indústria de laticínios e carne. O diagnóstico clínico é baseado nos sintomas e exames laboratorias confirmatórios são necessários. Atualmente, todos os ensaios de diagnósticos têm sido desenvolvidos com ELISA indireto de antígenos totais baseado em epítopos múltiplos. Em uma investigação pioneira demonstramos o uso de motivos críticos de um epítopo como biomarcador para a aplicação em imunossensores. Mimotopos do epítopo funcional da proteína MSP1a foram obtidos por meio de Phage Display, após três ciclos de seleção, de uma biblioteca randômica de peptídeos 12-mer contra o anticorpo monoclonal 15D2. Trinta e nove clones foram selecionados aleatoriamente, sequenciados, traduzidos e alinhados com a sequência nativa. Foi obtida a sequência consenso SxSSQSEASTSSQLGA, que está localizada na extremidade C-terminal do motivo repetitivo de 28-aa da proteína MSP1a, mas o alinhamento e a variação das sequências entre os mimotopos nos permitiu mapear o motivo crítico STSSxL dentro da sequência consenso. Com base nesses resultados, dois peptídeos foram quimicamente sintetizados; um baseado no motivo crítico (STSSQL, Am1) e o outro baseado na sequência consenso alinhada com o epítopo nativo (SEASTSSQLGA, Am2). Soro de 24 animais infectados e 52 animais saudáveis foram testados por ELISA quanto à reatividade contra Am1 e Am2, que apresentou sensibilidades de 96% e 100%, respectivamente. O peptide Am1 foi incorporado a um bioeletrodo (grafite modificado com ácido poli-3-hidroxyfenilacético) e a detecção direta de soro foi demonstrada por impedância, voltametria de pulso diferencial e microscopia de força atômica. O sistema de sensor eletroquímico provou ser altamente eficaz em soro de animais positivos de negativos. Este imunossensor foi altamente sensível e seletivo para IgG positiva, contaminantes não afetaram as medições, e foram baseados e um sistema simples, rápido e reprodutível.
Doutor em Genética e Bioquímica

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Orner, Sherko A. "Functional characterisation of Neisseria meningitidis autotransporters MSPA and APP." Thesis, University of Nottingham, 2007. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.490049.

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Gaja, Tomáš. "Návrh anténní řady pro MSPS radar pracující v pásmu L." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2017. http://www.nusl.cz/ntk/nusl-316426.

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This thesis deals with the design of an antenna array for the MSPS Radar L band application. The introduction covers a research for a suitable antenna element which can be used as an element of steerable antenna array. The control of the main beam is enabled in the vertical plane. Based on a presented theory, a slotted waveguide antenna array with an omnidirectional radiation in the vertical plane is designed. The operating frequency is set to 1 367.5 MHz. Slotted array achieves 20° width of the main beam in elevation plane. The achieved gain of the array is 9.15 dBi. Further attention of this work is focused on the beam steering that is allowed by diode switching. The last part of the thesis presents manufacturing process of the designed model. The CST Microwave Studio software was used for the antenna array designing process.

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Ekbäck, Maria Palmetun. "Hirsutism and quality of life with aspects on social support, anxiety and depression." Doctoral thesis, Örebro universitet, Institutionen för hälsovetenskap och medicin, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:oru:diva-32020.

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Hirsutism is excessive hair growth in women. The prevalence is estimated at 5%. The aim of this thesis was to describe different aspects of how life is affected for women suffering from hirsutism. Both qualitative and quantitative methods were used. Study I showed that hirsutism deeply affects women’s experiences of their bodies in a negative way and was experienced as a life sorrow. In Study II the patient-physician relationship was described. The patient-physician relationship from the patient’s perspective was suboptimal, as most meetings included feelings of being rejected and even humiliation. In Study III the aim was to translate and psychometrically evaluate an instrument that measures perceived social support, “The Multidimensional Scale of Perceived Social Support” (MSPSS). The translation was performed according to WHO:s official process, and validation was performed in a sample that consisted of 281 participants, 127 women with hirsutism (main sample) and 154 nursing students. MSPSS had good psychometric properties with regard to factor structure, construct validity, internal consistency and reproducibility. Study IV described different aspects of HRQoL in the main sample, the correlation of anxiety, depression, level of hairiness, age and BMI. The F-G scores were dichotomized into minor (F-G ≤14) and major (F-G≥15) hair growth. Higher levels of hair growth were significantly correlated to a lower level of QoL measured by DLQI, EQ-5D and symptoms of both anxiety and depression measured by HADS. Study V investigated if social support was associated with quality of life and outcome of HRQoL compared to a reference group of women (n=1115). SF-36, the MSPSS and the F-G scale were used. Compared to the reference group, women with hirsutism reported lower quality of life in all dimensions of SF-36 (p<0.01) The dimension most affected was vitality (VT=41.2), which had a lower value than has been reported for patients with MS and myasthenia gravis. A Multiple Regression Analysis showed a significant relation between quality of life and social support, indicating its importance for the ability to adapt, in spite of low quality of life.

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Ricci, Luca. "Design of a 12-bit 200-MSps SAR Analog-to-Digital converter." Thesis, KTH, Skolan för elektroteknik och datavetenskap (EECS), 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-284559.

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The Successive Approximation (SAR) Analog-to-Digital converter is one of the most energy-efficient A/D converter. In this thesis, the development of a SAR ADC in a 28-nm CMOS technology based on charge redistribution is presented.The implemented SAR ADC uses a switching procedure based on a modified version of the mono- tonic switching algorithm to reduce the switching energy and area of the DAC. The DAC is a binary- weighted array of unit capacitors. A unit custom capacitor has been designed with a value of 0.8 fF to reduce the DAC energy consumption. Two comparators have been implemented, a dynamic comparator and a static comparator. The dynamic implementation allows to obtain better performance. Therefore, the dynamic comparator is chosen for the SAR ADC. The sampling switches are bootstrapped to reduce the non-linearity introduced when the input signal is sampled. The SAR operations are controlled by an asynchronous logic implemented as a behavioural model in Verilog-A.The effect of the designed circuits on the linearity of the converter is assessed with the integral non- linearity (INL) and differential non-linearity (DNL). Moreover, the performance of the ADC are assessed in terms of signal-to-noise-and-distortion ratio (SNDR). The co-simulation of Verilog-A behavioural models with circuit schematics allowed to evaluate the effect of each block on the overall performance of the ADC. The co-simulations show that the ADC is able to achieve an ENOB of 10.9 at a sampling rate of 200 MSps with a power consumption of 2.83 mW. The resulting FoM is 7.4 fJ/conv-step.
SAR (Analog-Digital-omvandlaren) är en av de mest energieffektiva omvandlare. I den här avhandlingen är utvecklingen av en SAR ADC i en 28-nm CMOS-teknik baserad på laddning omfördelning presen- teras.Den implementerade SAR ADC använder en omkopplingsprocedur baserad på en modifierad version av den monotoniska omkopplingsalgoritm för att reducera omkopplingsenergin och DAC-området. DAC är en binärviktad matris med enhetskondensatorer. En anpassad kondensator för enheten har utformats med ett värde av 0,8 fF för att minska DAC-energiförbrukningen. Två komparatorer har implementerats, en dynamisk komparator och en statisk komparator. Den dynamiska implementering gör det möjligt att få bättre prestanda. Därför väljs den dynamiska komparatorn SAR ADC. Provtagningsomkopplarna startas upp för att minska icke-lineariteten introduceras när insignalen samplas. SAR-operationerna styrs av en asynkron logik implementerad som en beteendemodell i Verilog-A.Effekten av de designade kretsarna på konverterarens linearitet bedöms med integralen icke-linearitet (INL) och differentiell icke-linearitet (DNL). Dessutom är ADC: s prestanda bedömdes i termer av signal-till-brus-och-distorsionsförhållande (SNDR). Samsimulering av Verilog-A beteendemodeller och scheman tillåts utvärdera effekten av varje block på prestandan hos ADC. Omvandlaren kan uppnå en ENOB på 10,9 med en samplingshastighet på 200 MSps, vilket resulterar i en FoM eller 7,4 fJ / konv.- steg.

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Huff, Jason. "Functional determinants of the porin MspA and its role in permeabilizing mycobacterial outer membranes." Thesis, Birmingham, Ala. : University of Alabama at Birmingham, 2010. https://www.mhsl.uab.edu/dt/2010p/huff.pdf.

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Khairalla, Ahmed Samir Mohammed. "Identification of host immune cell receptors mediating the binding of meningococcal App/MspA proteins." Thesis, University of Nottingham, 2012. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.575478.

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Neisseria meningitidis, a major cause of bacterial meningitis and septicemia, secretes multiple virulence factors to the extracellular environment, including the adhesion and penetration protein (App) and the meningococcal serine protease A (MspA). Both proteins belong to the serine protease auto transporter family and have the capacity to adhere to host epithelial and endothelial cells. Besides, both proteins have been previously shown to be internalized and translocated to the nuclei of human brain microvascular endothelial cells and dendritic cells (DCs), where they modulate gene expression. However, host cell receptors, endocytic pathways, nuclear translocation mechanisms, and gene expression modulation associated with App or MspA are poorly defined. In an effort to address this knowledge gap, recombinant forms of the passenger domains of both proteins were purified under native conditions using cold shock expression technology. The purified proteins, designated rpdApp and rpdMspA, were used to raise polyclonal, affinity-purified guinea pig antisera. These antibodies facilitated the investigation of interactions between both bacterial proteins and host cell molecules. One of these interactions highlighted in the present study is the entry of rpdApp and rpdMspA into human monocyte-derived immature DCs via receptor-mediated endocytosis. In vitro internalization and blocking assays showed that mannose receptor (MR) and transferrin receptor 1 are involved in the uptake of both proteins into DCs. Besides, two different approaches revealed that the binding of rpdApp and rpdMspA to MR is mediated specifically by the C-type lectin-like carbohydrate recognition domains 4':'7 (CTLD4-7). Additionally, the recognition of both bacterial proteins by these domains was shown to be calcium dependent, in addition to being inhibitable by D-mannose or L- fucose, but not D-galactose. Using gel overlay (Far-Western) analysis, rpdApp and rpdMspA were shown to interact with two nucleocytoplasmic shuttling proteins, namely heat shock protein 70 (Hsp70) and galectin-3 (Gal-3), with some data suggesting that interactions with Gal-3 may be mediated by its carbohydrate-recognition domain. Hsp70, which also interacts with the bacterial proteins, is characterized by possessing lectinic activity towards O-linked ~-N-acetylglucosamine (O-G1cNAc). Given the binding of App and MspA to different lectins, this study sought to examine the glycosylation status of both proteins, with a focus on O-GlcNAc modifications and their possible interplay with phosphorylation. Using in silico prediction methods, multiple sites within each protein were identified as potential sites for phosphorylation, O-GlcNAc modification, and alternative phosphorylation/O-GlcNAc modification. These predictions were supported by a series of Western blot analyses, which reproducibly showed that several meningococcal secreted proteins, including those of the expected size of App or MspA (115-120 kDa), are modified by both O-GlcNAc and tyrosine phosphorylation. The identity of the 115- to 120-kDa proteins was confirmed by stripping and reprobing the blots with antibodies against rpdApp and rpdMspA. However, attempts to detect these post-translational modifications in a sample of meningococcal secreted proteins tagged with TAMRA-alkyne by tandem mass spectrometry were unsuccessful. This may be attributed to the suppressive effect of the attached tag on trypsin cleavage and/or ionization efficiency. Finally, the results of Far-Western and ELISA assays clearly demonstrated the interactions of rpdApp or rpdMspA with various histone subunits. Additionally, both proteins were shown to have trypsin-like serine protease activity and to be capable of cleaving recombinant histone H3.1 in vitro. The proteolytic activity of both proteins on H3.l was shown to be specifically abolished by pre-incubation with serine protease inhibitors. Collectively, these results extend our understanding of the virulence potential of App and MspA beyond the adhesive or invasive functions, and reveal their capacity to participate in multiple interactions with host molecules. Besides, the current findings provide the basis for future studies to explore the epigenetic alterations induced by both proteins. Hopefully, the information gained will provide new insights into meningococcal pathogenesis and will help in the development of improved therapeutic approaches against the disease.

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Lesniewska, Karolina. "New tools reveal interaction determinants and post-mitotic function of crucial microtubule regulators." Thesis, University of Edinburgh, 2014. http://hdl.handle.net/1842/9371.

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Microtubules are a major constituent of the cytoskeleton in all eukaryotic cells. They are essential for cell morphogenesis and motility. Specifically in the dividing cells, microtubules form the spindle which segregates chromosomes. Microtubule plus ends constantly switch between phases of growth and shrinkage which is necessary for microtubule reorganization and thus their function. Importantly, microtubule dynamics are highly regulated by microtubule-associated proteins (MAPs). EB1 and Mini spindles (Msps) are unique amongst MAPs because they bind and track growing microtubule plus ends autonomously. Although essential for cell division and thus highly expressed in dividing cells, EB1 and Msps are also abundant in differentiated cells. However, to identify post-mitotic roles of proteins essential for cell division, particularly in context of a multicellular organism, is a challenge requiring new tools which I aimed to develop in my project. Since EB1 acts by recruiting MAPs to the microtubule plus ends, I generated short peptides which bind to Drosophila EB1 to block interactions with these MAPs. I showed that an EB1-MAP interaction was disturbed in Drosophila S2 cultured cells and expressing these peptides in developing Drosophila reduced fly viability. Further screening and analysis of peptides interacting with fly EB1 and its human homologues uncovered sequence determinants promoting strong binding and specificity. To uncover Msps function, I generated a msps temperature sensitive mutant and found that Msps is essential for neuromuscular function in developing Drosophila. This study showed that the regulation of microtubule dynamics has crucial functions at the whole organism level. These new tools allow the roles of microtubule regulation to be dissected in developing organisms.

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Books on the topic "MSPSHA"

1

Body, Scottish Parliamentary Corporate. SPCB/MSPs. [S.l.]: SPCB, 1999.

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Scotland. Parliament. Public Information Service., ed. You and your MSPs. Edinburgh: Public Information Service, 1999.

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Judy, Blair, Barrett Ruth, Shierling Maryellen, and United States. Employment Standards Administration. Wage and Hour Division, eds. Conducting MSPA housing inspection: Test book. [Washington, D.C.?]: U.S. Dept. of Labor, Employment Standards Administration, Wage and Hour Division, 1990.

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Shierling, Maryellen. Conducting MSPA housing inspections: Task book. Washington, D.C.?]: U.S. Department of Labor, Employment Standards Administration, Wage and Hour Division, 1990.

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Shierling, Maryellen. Conducting MSPA housing inspection: Test book. Washington, D.C.?]: U.S. Department of Labor, Employment Standards Administration, Wage and Hour Division, 1990.

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Judy, Blair, Barrett Ruth, Shierling Maryellen, and United States. Employment Standards Administration. Wage and Hour Division, eds. Conducting MSPA housing inspections: Resource book. [Washington, D.C.?]: U.S. Dept. of Labor, Employment Standards Administration, Wage and Hour Division, 1990.

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Matthews, Joseph R. Service providers: ASPs, ISPs, MSPs, and WSPs : a Wiley tech brief. New York: John Wiley & Sons, 2002.

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MSPCA Humane Education Seminar (2nd 1988 Jalal Shuja Centre). MSPCA Second Humane Education Seminar: The Jalal Shuja Centre, 17th-19th August 1988 : a summary of the proceedings, talks, and selected papers on humane education, plant and animal ecosystems and the environment. Mauritius: SPCA, 1988.

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United States. Congress. House. Committee on Education and the Workforce. Subcommittee on Workforce Protections. The effect of the Fair Labor Standards Act on Amish families and H.R. 2038, the MSPA Clarification Act: Hearing before the Subcommittee on Workforce Protections of the Committee on Education and the Workforce, House of Representatives, One Hundred Fifth Congress, second session, hearing held in Washington, DC, April 21, 1998. Washington: U.S. G.P.O., 1998.

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United States. Congress. House. Committee on Education and the Workforce. Subcommittee on Workforce Protections. H.R. 1886, MSPA Clarification Act, H.R. 2757, housing opportunities for migrant employees, H.R. 3121, amending the Migrant and Seasonal Agricultural Worker Protection Act: Hearing before the Subcommittee on Workforce Protections of the Committee on Education and the Workforce, House of Representatives, One Hundred Sixth Congress, first session, hearing held in Washington, DC, October 21, 1999. Washington: U.S. G.P.O., 2000.

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Book chapters on the topic "MSPSHA"

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Han, Xue, Yuanyuan Wu, and Jie Zheng. "Pair Comparison Between Chinese MSPs and American MSPs." In Disruptive Innovation through Digital Transformation, 69–84. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-3944-2_5.

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Han, Xue, Yuanyuan Wu, and Jie Zheng. "Emerging E-Health MSPs in China." In Disruptive Innovation through Digital Transformation, 49–67. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-3944-2_4.

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Wang, Xiangzhan, Bijiang Chen, Jun Niu, Wen Luo, Yunchao Guan, Jun Zhang, and Kejun Wu. "A 12-b 100-MSps Pipelined ADC." In Electrical Engineering and Control, 669–76. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-21765-4_83.

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Huang, Di, Zhijie Wang, Jun Wang, and ZiYao Liu. "Parallel Symbol Timing Recovery Using FPGA for 600 Msps QPSK." In Communications and Networking, 216–25. Cham: Springer International Publishing, 2018. http://dx.doi.org/10.1007/978-3-319-78130-3_23.

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Cococcioni, Marco. "mspMEA: The Microcones Separation Parallel Multiobjective Evolutionary Algorithm and Its Application to Fuzzy Rule-Based Ship Classification." In Recent Advances in Computational Intelligence in Defense and Security, 445–65. Cham: Springer International Publishing, 2015. http://dx.doi.org/10.1007/978-3-319-26450-9_17.

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Puozaa, Doris K., Alhassan Nuhu Jinbaani, Desmond S. Adogoba, Douglas Busagri, Masawudu Abdul Rasheed, Abdul Rashid Issah, and Richard Oteng-Frimpong. "Enhancing Access to Quality Seed of Improved Groundnut Varieties Through Multi-Stakeholder Platforms in Northern Ghana." In Enhancing Smallholder Farmers' Access to Seed of Improved Legume Varieties Through Multi-stakeholder Platforms, 65–79. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8014-7_5.

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AbstractOne of the main constraints to groundnut production has been the use of low yielding varieties, susceptible to biotic and abiotic stresses. Multi-stakeholder platforms (MSPs) have been used by agricultural research organizations to help make their research more relevant and to facilitate the adaptation and dissemination of findings. In the implementation of TLIII project in Ghana, eight MSPs were set up with the objective to improve groundnut productivity among actors through improved access to varieties and related technologies. Through trainings, demonstrations and field days, the platform members totalling 347 (55% being female) were exposed to two existing improved groundnut varieties and several candidate varieties which were yet to be released. The actors have become important sources of high-quality groundnut seed in their communities and beyond with the production of 5 tons of seed of the new varieties. The seed farms where improved varieties were planted and managed using good agronomic practices (GAPS) yielded up to 1.6 tons/ha, 70% higher than the use of their old varieties and practices on their personal farms which gave less than 0.5 tons/ha. This form of experiential learning was observed to be very useful in the willingness of platform members to uptake technology. As unintended benefits, through the use of funds raised by the associations, the sanitation and health of some members and their households were improved, and many engaged in non-farm businesses. Going forward, good conflict resolution skills and access to funds for supporting platform activities and members’ individual interests would be very important in the sustainability of these platforms.

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Monyo, Emmanuel S., Essegbemon Akpo, Chris O. Ojiewo, and Rajeev K. Varshney. "A Cross-Case Analysis of Innovation Platform Experiences in Seven Countries in West and East Africa and South Asia." In Enhancing Smallholder Farmers' Access to Seed of Improved Legume Varieties Through Multi-stakeholder Platforms, 185–97. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-8014-7_13.

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AbstractThe Bill and Melinda Gates Foundation (BMGF) funded Tropical Legumes (TL III) project was implemented in seven sub-Saharan Africa countries (Burkina Faso, Ghana, Mali, Nigeria, Ethiopia, Tanzania, and Uganda) and South Asia (India). Shortage of seed of improved varieties has been identified as the greatest hindrance to farmer adoption of new agricultural technologies developed through this project. This chapter compares the different approaches followed by different countries in the establishment of Multi-Stakeholder Platforms (MSPs) for supply of improved legume seed to farmers. Achievements from this initiative are mixed and multi-dimensional. The details herein provide the reader with insights on the level of success of innovation platforms in the different countries and implications for agricultural technology dissemination to smallholder farmers. Key achievements include strengthened linkages among various legume seed value chain actors, participation of several cadres of seed producers in a decentralized system resulting into significant increase in the production of certified and quality declared seed of legumes, and rapid adoption and use of newly released varieties by smallholder farmers. As for those areas where the initiative did not produce the desired results, it is a testament that unless a well thought-out inclusive and comprehensive approach which defines the critical roles of each player in the value chain is developed, current seed shortages will continue, eroding emerging market opportunities and good intentions of development partners. The reader is directed to individual chapters for details of the process followed by each country/crop in the establishment of MSPs, their composition, key achievements, challenges, and lessons for overall improvement of the national legume seed systems.

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Monika Mathur, Ghanshyam Singh, and S. K. Bhatnagar. "An Analytical Approach for Accurate Design of MSPA Using Dielectric Constant Engineering (DCE)." In Proceedings of the International Conference on Recent Cognizance in Wireless Communication & Image Processing, 107–14. New Delhi: Springer India, 2016. http://dx.doi.org/10.1007/978-81-322-2638-3_12.

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Brandt, Brian, and Joseph Lutsky. "A 10-bit, 20–30 MSPS CMOS Subranging ADC with 9.5 Effective Bits at Nyquist." In Analog Circuit Design, 75–94. Boston, MA: Springer US, 2000. http://dx.doi.org/10.1007/978-1-4757-3198-9_4.

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Steenkamp, Yolande, Dominik Fischer, and Derick de Jongh. "Leadership in Multi-stakeholder Partnerships (MSPs) that Address the SDGs: Relational Leadership and Ubuntu in Dialogue." In Relational Economics and Organization Governance, 213–27. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-86526-9_11.

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Conference papers on the topic "MSPSHA"

1

Wang, Zhelong, and Ying Chen. "ECG signal compression based on MSPIHT algorithm." In 2008 International Conference on Technology and Applications in Biomedicine (ITAB). IEEE, 2008. http://dx.doi.org/10.1109/itab.2008.4570530.

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Eriksson, Joakim, Fredrik Österlind, Niclas Finne, Nicolas Tsiftes, Adam Dunkels, Thiemo Voigt, Robert Sauter, and Pedro José Marrón. "COOJA/MSPSim: interoperability testing for wireless sensor networks." In 2nd International ICST Conference on Simulation Tools and Techniques. ICST, 2009. http://dx.doi.org/10.4108/icst.simutools2009.5637.

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Ibrahim, Raied A. R., and Mustapha C. E. Yagoub. "Practical novel design component of microstrip patch slot antenna MSPSA for RFID applications." In 2010 IEEE 23rd Canadian Conference on Electrical and Computer Engineering - CCECE. IEEE, 2010. http://dx.doi.org/10.1109/ccece.2010.5575105.

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Zhang Haifeng and Wang Yuesheng. "Improved MSPCA with application to process monitoring." In International Technology and Innovation Conference 2006 (ITIC 2006). IEE, 2006. http://dx.doi.org/10.1049/cp:20061149.

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Lakshmi, S. Sathiya, and M. Vanitha Lakshmi. "Segmentation and compression of medical image using MSPIHT in telemedicine application." In 2014 International Conference on Information Communication and Embedded Systems (ICICES). IEEE, 2014. http://dx.doi.org/10.1109/icices.2014.7033975.

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Xia, L. Y., H. T. Pan, Y. J. Cai, X. F. Sun, and Li Yu. "Fault detection for chemical process based on improved MSPCA." In 2010 8th World Congress on Intelligent Control and Automation (WCICA 2010). IEEE, 2010. http://dx.doi.org/10.1109/wcica.2010.5554749.

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Xu Tao. "Sensor fault diagnosis and data reconstruction based on MSPCA." In 2008 Chinese Control Conference (CCC). IEEE, 2008. http://dx.doi.org/10.1109/chicc.2008.4605055.

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Eriksson, Joakim, Fredrik Osterlind, Thiemo Voigt, Niclas Finne, Shahid Raza, Nicolas Tsiftes, and Adam Dunkels. "Demo abstract: Accurate power profiling of sensornets with the COOJA/MSPsim simulator." In 2009 IEEE 6th International Conference on Mobile Adhoc and Sensor Systems (MASS). IEEE, 2009. http://dx.doi.org/10.1109/mobhoc.2009.5337011.

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Miksch, Markus, Martin Steiner, Michael Miksch, and Alexander Meschtscherjakov. "Motion Sickness Prevention System (MSPS)." In AutomotiveUI'16: 8th International Conference on Automotive User Interfaces and Interactive Vehicular Applications. New York, NY, USA: ACM, 2016. http://dx.doi.org/10.1145/3004323.3004340.

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Cabalkova, Petra, David Kubal, Martin Pelant, Radek Plsek, and Vojtech Stejskal. "Aspects of target detection in MSPSR system under clutter conditions." In 2014 15th International Radar Symposium (IRS). IEEE, 2014. http://dx.doi.org/10.1109/irs.2014.6869190.

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Reports on the topic "MSPSHA"

1

Owili, Jackie, Katherine Pittore, and Eunice Likoko. Multi-stakeholder platforms (MSPs) and food systems policies in Bangladesh, Ethiopia, Nigeria and Vietnam : mapping of MSPs and policies to promote a shift towards a food system framing for healthier diets. Wageningen: Wageningen Centre for Development Innovation, 2021. http://dx.doi.org/10.18174/549177.

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Palmer, Guy, Varda Shkap, Wendy Brown, and Thea Molad. Control of bovine anaplasmosis: cytokine enhancement of vaccine efficacy. United States Department of Agriculture, March 2007. http://dx.doi.org/10.32747/2007.7695879.bard.

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Anaplasmosis an arthropod-born disease of cattle caused by the rickettsia Anaplasma marginale and is an impediment to efficient production of healthy livestock in both Israel and the United States. Currently the only effective vaccines are derived from the blood of infected cattle. The risk of widespread transmission of both known and newly emergent pathogens has prevented licensure of live blood-based vaccines in the U.S. and is a major concern for their continued use in Israel. Consequently development of a safe, effective vaccine is a high priority. In this collaborative project we focused on two approaches to vaccine development. The first focused o n improving antigen delivery to livestock and specifically examined how DNA vaccines could be improved to enhance priming and expansion of the immune response. This research resulted in development and testing of two novel vaccine delivery systems--one that targeted antigen spread among dendritic cells (the key cell in priming immune responses and a follow-on construct that also specifically targeted antigen to the endosomal-lysosomal compartment the processing organelle within the dendritic cell that directs vaccine antigen to the MHC class ll-CD4* T cell priming pathway). The optimized construct targeting vaccine antigen to the dendritic cell MHC class II pathway was tested for ability to prime A. marginale specific immune responses in outbred cattle. The results demonstrated both statistically significant effects of priming with a single immunization, continued expansion of the primary immune response including development of high affinity lgG antibodies and rapid recall of the memory response following antigen challenge. This portion of the study represented a significant advance in vaccine delivery for livestock. Importantly the impact of these studies is not limited to A. marginale a s the targeting motifs are optimized for cattle and can be adapted to other cattle vaccinations by inserting a relevant pathogen-specific antigen. The second approach (which represented an addition to the project for which approval was requested as part of the first annual report) was a comparative approach between A . marginale and the Israel A . centrale vaccines train. This addition was requested as studies on Major Surface Protein( MSP)- 2 have shown that this antigen is highly antigenically variable and presented solely as a "static vaccine" antigen does not give cross-strain immunity. In contrast A. . centrale is an effective vaccine which Kimron Veterinary institute has used in the field in Israel for over 50 years. Taking advantage of this expertise, a broad comparison of wild type A. marginale and vaccine strain was initiated. These studies revealed three primary findings: i) use of the vaccine is associated with superinfection, but absence of clinical disease upon superinfection with A. marginale; ii) the A. centrale vaccine strain is not only less virulent but transmission in competent in Dermacentor spp. ticks; and iii) some but not all MSPs are conserved in basic orthologous structure but there are significant polymorphisms among the strains. These studies clearly indicated that there are statistically significant differences in biology (virulence and transmission) and provide a clear path for mapping of biology with the genomes. Based on these findings, we initiated complete genome sequencing of the Israel vaccine strain (although not currently funded by BARD) and plant to proceed with a comparative genomics approach using already sequenced wild-type A. marginale. These findings and ongoing collaborative research tie together filed vaccine experience with new genomic data, providing a new approach to vaccine development against a complex pathogen.

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