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Tournier, J.-Donald, Fernando Calamante, and Alan Connelly. "MRtrix: Diffusion tractography in crossing fiber regions." International Journal of Imaging Systems and Technology 22, no. 1 (February 14, 2012): 53–66. http://dx.doi.org/10.1002/ima.22005.
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Noriega-Gonzalez, David C., Jesús Crespo, Francisco Ardura, Juan Calabia-del Campo, Carlos Alberola-Lopez, Rodrigo de Luis-García, Alberto Caballero-García, and Alfredo Córdova. "Cerebral White Matter Connectivity in Adolescent Idiopathic Scoliosis: A Diffusion Magnetic Resonance Imaging Study." Children 9, no. 7 (July 10, 2022): 1023. http://dx.doi.org/10.3390/children9071023.
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Adolescent idiopathic scoliosis (AIS) is characterized by the radiographic presence of a frontal plane curve, with a magnitude greater than 10° (Cobb technique). Diffusion MRI can be employed to assess the cerebral white matter. The aim of this study was to analyze, by means of MRI, the presence of any alteration in the connectivity of cerebral white matter in AIS patients. In this study, 22 patients with AIS participated. The imaging protocol consisted in T1 and diffusion-weighted acquisitions. Based on the information from one of the diffusion acquisitions, a whole brain tractography was performed with the MRtrix tool. Tractography is a method to deduce the trajectory of fiber bundles through the white matter based on the diffusion MRI data. By combining cortical segmentation with tractography, a connectivity matrix of size 84 × 84 was constructed using FA (fractional anisotropy), and the number of streamlines as connectomics metrics. The results obtained support the hypothesis that alterations in cerebral white matter connectivity in patients with adolescent idiopathic scoliosis (AIS) exist. We consider that the application of diffusion MRI, together with transcranial magnetic stimulation neurophysiologically, is useful to search the etiology of AIS.
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Coenen, Volker A., Bastian E. Sajonz, Peter C. Reinacher, Christoph P. Kaller, Horst Urbach, and M. Reisert. "A detailed analysis of anatomical plausibility of crossed and uncrossed streamline rendition of the dentato-rubro-thalamic tract (DRT(T)) in a commercial stereotactic planning system." Acta Neurochirurgica 163, no. 10 (June 28, 2021): 2809–24. http://dx.doi.org/10.1007/s00701-021-04890-4.
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Abstract Background An increasing number of neurosurgeons use display of the dentato-rubro-thalamic tract (DRT) based on diffusion weighted imaging (dMRI) as basis for their routine planning of stimulation or lesioning approaches in stereotactic tremor surgery. An evaluation of the anatomical validity of the display of the DRT with respect to modern stereotactic planning systems and across different tracking environments has not been performed. Methods Distinct dMRI and anatomical magnetic resonance imaging (MRI) data of high and low quality from 9 subjects were used. Six subjects had repeated MRI scans and therefore entered the analysis twice. Standardized DICOM structure templates for volume of interest definition were applied in native space for all investigations. For tracking BrainLab Elements (BrainLab, Munich, Germany), two tensor deterministic tracking (FT2), MRtrix IFOD2 (https://www.mrtrix.org), and a global tracking (GT) approach were used to compare the display of the uncrossed (DRTu) and crossed (DRTx) fiber structure after transformation into MNI space. The resulting streamlines were investigated for congruence, reproducibility, anatomical validity, and penetration of anatomical way point structures. Results In general, the DRTu can be depicted with good quality (as judged by waypoints). FT2 (surgical) and GT (neuroscientific) show high congruence. While GT shows partly reproducible results for DRTx, the crossed pathway cannot be reliably reconstructed with the other (iFOD2 and FT2) algorithms. Conclusion Since a direct anatomical comparison is difficult in the individual subjects, we chose a comparison with two research tracking environments as the best possible “ground truth.” FT2 is useful especially because of its manual editing possibilities of cutting erroneous fibers on the single subject level. An uncertainty of 2 mm as mean displacement of DRTu is expectable and should be respected when using this approach for surgical planning. Tractographic renditions of the DRTx on the single subject level seem to be still illusive.
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Fayaz, Mohamed Salah, Gerges Attia Demian, Wael Moftah Fathallah, Heba El-Sayed Eissa, Mustafa Shawki El-Sherify, Sadeq Abozlouf, Thomas George, and Suzanne Mona Samir. "Significance of Magnetic Resonance Imaging–Assessed Tumor Response for Locally Advanced Rectal Cancer Treated With Preoperative Long-Course Chemoradiation." Journal of Global Oncology 2, no. 4 (August 2016): 216–21. http://dx.doi.org/10.1200/jgo.2015.001479.
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Purpose To study the predictive and prognostic value of magnetic resonance imaging (MRI)–assessed tumor response after long-course neoadjuvant therapy for locally advanced rectal cancer. Methods This study included 79 patients who had T3 or T4 and/or N+ rectal cancer treated with long-course neoadjuvant chemoradiation. MRI-assessed tumor regression grade (mrTRG) was assessed in 64 patients. MRIs were reviewed by the study radiologist. Surgical and pathologic reports for those who underwent surgery were reviewed. Disease-free survival (DFS) was estimated. Progression during therapy, local relapse, metastasis, and death resulting from the tumor were classified as events. Statistical significance was calculated. Results In 11 patients, the tumor completely disappeared on MRI; that is, it had an mrTRG of 1. All but one patient, who chose deferred surgery, had a complete pathologic response (pCR), with a positive predictive value of nearly 100%. Of the 20 patients who had an mrTRG of 2 on MRI, six had a pCR. mrTRG 3, mrTRG 4, and mrTRG 5 were detected in 24, six, and three patients, respectively, of whom only one patient had a pCR. The 2-year DFS was 77%. The mrTRG was significant for DFS. The 2-year DFS was 88% for patients with a good response versus 66% for those with a poor response (P = .046). Conclusion MRI-assessed complete tumor response was strongly correlated with pCR and, therefore, can be used as a surrogate marker to predict absence of viable tumor cells. Our results can be used to implement use of mrTRGs in larger prospective correlative studies as a tool to select patients for whom deferred surgery may be appropriate. Also, those with a poor response may be offered further treatment options before definitive surgery.
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McCormick, Susan P., and Nancy J. Alexander. "Myrothecium roridum Tri4 encodes a multifunctional oxygenase required for three oxygenation steps." Canadian Journal of Microbiology 53, no. 5 (May 2007): 572–79. http://dx.doi.org/10.1139/w07-025.
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The biosyntheses of both macrocyclic trichothecenes in Myrothecium roridum and simple trichothecenes in Fusarium species begin with the cyclization of farnesyl pyrophosphate to form the sesquiterpene hydrocarbon trichodiene. A previous study showed that Myrothecium has a cluster of 3 genes that are homologous with Fusarium trichothecene genes: Tri4, a P450 oxygenase; Tri5, the sesquiterpene cyclase; and Tri6, a zinc-finger regulatory gene. Fusarium graminearum Tri4 (FgTri4) and M. roridum MrTri4 (MrTri4) have 66.9% identity. In this study, MrTri4 was expressed in Fusarium verticillioides . Liquid cultures of transformant strains expressing MrTri4 converted exogenous trichodiene to isotrichodiol, indicating that MrTri4 controls 3 oxygenation steps and that the product of MrTRI4 is isotrichodiol.
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Zheng, Huiming, Zengtao Zhong, Xin Lai, Wen-Xin Chen, Shunpeng Li, and Jun Zhu. "A LuxR/LuxI-Type Quorum-Sensing System in a Plant Bacterium, Mesorhizobium tianshanense, Controls Symbiotic Nodulation." Journal of Bacteriology 188, no. 5 (March 1, 2006): 1943–49. http://dx.doi.org/10.1128/jb.188.5.1943-1949.2006.
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ABSTRACT The ability of rhizobia to symbiotically fix nitrogen from the atmosphere when forming nodules on their plant hosts requires various signal transduction pathways. LuxR-LuxI-type quorum-sensing systems have been shown to be one of the players in a number of rhizobium species. In this study, we found that Mesorhizobium tianshanense, a moderate-growth Rhizobium that forms nodules on a number of licorice plants, produces multiple N-acyl homoserine lactone (AHL)-like molecules. A simple screen for AHL synthase genes using an M. tianshanense genomic expression library in Escherichia coli, coupled with a sensitive AHL detector, uncovered a LuxI-type synthase, MrtI, and a LuxR-type regulator, MrtR, in M. tianshanense. Deletions of the mrtI or mrtR locus completely abolished AHL production in M. tianshanense. Using lacZ transcriptional fusions, we found that expression of the quorum-sensing regulators is autoinduced, as mrtI gene expression requires MrtR and cognate AHLs and mrtR expression is dependent on AHLs. Compared with the wild-type strains, quorum-sensing-deficient mutants showed a marked reduction in the efficiency of root hair adherence and, more importantly, were defective in nodule formation on their host plant, Glycyrrhiza uralensis. These data provide strong evidence that quorum sensing plays a critical role in the M. tianshanense symbiotic process.
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Muller, Jennifer, Mahdi Alizadeh, Feroze B. Mohamed, Jonathan Riley, John J. Pearce, Benjamin Trieu, Tsao-Wei Liang, Victor Romo, Ashwini Sharan, and Chengyuan Wu. "Clinically applicable delineation of the pallidal sensorimotor region in patients with advanced Parkinson’s disease: study of probabilistic and deterministic tractography." Journal of Neurosurgery 131, no. 5 (November 2019): 1520–31. http://dx.doi.org/10.3171/2018.7.jns18541.
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OBJECTIVEDeep brain stimulation (DBS) is an effective procedure in improving motor symptoms for patients with advanced Parkinson’s disease (PD) through the use of high-frequency stimulation. Although one of the most commonly used target sites for DBS, sensorimotor regions of the globus pallidus interna (GPi) have yet to be thoroughly described with advanced neuroimaging analysis in vivo for human subjects. Furthermore, many imaging studies to date have been performed in a research setting and bring into question the feasibility of their applications in a clinical setting, such as for surgical planning. This study compares two different tractography methods applied to clinically feasible acquisition sequences in identifying sensorimotor regions of the GPi and the subthalamic nucleus (STN) in patients with advanced PD selected to undergo DBS.METHODSSeven patients with refractory PD selected for DBS were examined by MRI. Diffusion images were acquired with an average acquisition time of 15 minutes. Probabilistic and deterministic tractography methods were applied to each diffusion-weighted data set using FSL and MRtrix, respectively. Fiber assignment was performed using combined sensorimotor areas as initiation seeds and the STN and GPi, separately, as inclusion masks. Corticospinal tracts were excluded by setting the cerebral peduncles as exclusion masks. Variability between proposed techniques was shown using center of gravity (CoG) coordinates.RESULTSDeterministic and probabilistic corticopallidal and corticosubthalamic pathways were successfully reconstructed for all subjects across all target sites (bilaterally). Both techniques displayed large connections between the sensorimotor cortex with the posterolateral aspect of the ipsilateral GPi and the posterosuperolateral aspect of the ipsilateral STN. The average variability was 2.67 mm, with the probabilistic method identifying the CoG consistently more posterior and more lateral than the deterministic method.CONCLUSIONSSuccessful delineation of the sensorimotor regions in both the GPi and STN is achievable within a clinically reasonable timeframe. The techniques described in this paper may enhance presurgical planning with increased accuracy and improvement of patient outcomes in patients undergoing DBS. The variability found between tracking techniques warrants the use of the probabilistic tractography method over the deterministic method for presurgical planning. Probabilistic tractography was found to have an advantage over deterministic tractography in its sensitivity, in accurately describing previously described tracts, and in its ability to detect a larger number of fibers.
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Meijer, Kim A., Martijn D. Steenwijk, Linda Douw, Menno M. Schoonheim, and Jeroen J. G. Geurts. "Long-range connections are more severely damaged and relevant for cognition in multiple sclerosis." Brain 143, no. 1 (November 15, 2019): 150–60. http://dx.doi.org/10.1093/brain/awz355.
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Abstract An efficient network such as the human brain features a combination of global integration of information, driven by long-range connections, and local processing involving short-range connections. Whether these connections are equally damaged in multiple sclerosis is unknown, as is their relevance for cognitive impairment and brain function. Therefore, we cross-sectionally investigated the association between damage to short- and long-range connections with structural network efficiency, the functional connectome and cognition. From the Amsterdam multiple sclerosis cohort, 133 patients (age = 54.2 ± 9.6) with long-standing multiple sclerosis and 48 healthy controls (age = 50.8 ± 7.0) with neuropsychological testing and MRI were included. Structural connectivity was estimated from diffusion tensor images using probabilistic tractography (MRtrix 3.0) between pairs of brain regions. Structural connections were divided into short- (length < quartile 1) and long-range (length > quartile 3) connections, based on the mean distribution of tract lengths in healthy controls. To determine the severity of damage within these connections, (i) fractional anisotropy as a measure for integrity; (ii) total number of fibres; and (iii) percentage of tract affected by lesions were computed for each connecting tract and averaged for short- and long-range connections separately. To investigate the impact of damage in these connections for structural network efficiency, global efficiency was computed. Additionally, resting-state functional connectivity was computed between each pair of brain regions, after artefact removal with FMRIB’s ICA-based X-noiseifier. The functional connectivity similarity index was computed by correlating individual functional connectivity matrices with an average healthy control connectivity matrix. Our results showed that the structural network had a reduced efficiency and integrity in multiple sclerosis relative to healthy controls (both P < 0.05). The long-range connections showed the largest reduction in fractional anisotropy (z = −1.03, P < 0.001) and total number of fibres (z = −0.44, P < 0.01), whereas in the short-range connections only fractional anisotropy was affected (z = −0.34, P = 0.03). Long-range connections also demonstrated a higher percentage of tract affected by lesions than short-range connections, independent of tract length (P < 0.001). Damage to long-range connections was more strongly related to structural network efficiency and cognition (fractional anisotropy: r = 0.329 and r = 0.447. number of fibres r = 0.321 and r = 0.278. and percentage of lesions: r = −0.219; r = −0.426, respectively) than damage to short-range connections. Only damage to long-distance connections correlated with a more abnormal functional network (fractional anisotropy: r = 0.226). Our findings indicate that long-range connections are more severely affected by multiple sclerosis-specific damage than short-range connections. Moreover compared to short-range connections, damage to long-range connections better explains network efficiency and cognition.
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Obradović, Nadežda, and Vida Ognjenović. "Kuća mrtvih mirisa." World Literature Today 70, no. 4 (1996): 991. http://dx.doi.org/10.2307/40152462.
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Shoja, A., H. Mazaheri, and R. Sarikhani. "λ -fuzzy Fixed Points In Fuzzy Mrtric Spaces." Journal of Mathematics and Computer Science 09, no. 04 (April 29, 2014): 332–41. http://dx.doi.org/10.22436/jmcs.09.04.09.
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Henri-Bellemare, Charlie, Raihaan Patel, Katie Lavigne, M. Mallar Chakravarty, and Martin Lepage. "M160. INVESTIGATING STRUCTURAL CONNECTIVITY CORRELATES OF VERBAL MEMORY DEFICITS AMONG FIRST-EPISODE PSYCHOSIS PATIENTS." Schizophrenia Bulletin 46, Supplement_1 (April 2020): S196—S197. http://dx.doi.org/10.1093/schbul/sbaa030.472.
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Abstract Background Verbal memory is one of the most affected cognitive domains in patients with schizophrenia and related psychoses. Several studies have found associations between cognitive abilities and white matter fractional anisotropy (FA) in schizophrenia; however, only a few tractography studies have investigated FA relative to verbal memory in patients with a first episode of psychosis (FEP) compared with healthy controls (HC). Although white matter tractography differences have been well established between chronic patients and HC, the direction of findings from FEP studies has been inconsistent. Thus, the present study aims to examine whole-brain white matter differences and its association with verbal memory in individuals with a FEP relative to HC using tractography. Methods Diffusion-weighted images were acquired on a 1.5T scanner for patients (n=65) and controls (n=54) at baseline. The Wechsler Memory Scale was used as a measure of verbal memory. Pre-processing was performed on a subject-by-subject basis using MRtrix. Diffusion tractography was generated using a probabilistic anatomically-constrained tractography algorithm, which constrains the reconstruction to specific biological priors. Furthermore, the spherical-deconvolution informed filtering of tractograms (SIFT) tool will be used to ensure the tractogram is biologically meaningful. This results in subject-specific connectomes defining the mean FA between two regions of interest that were defined using the Desikan- Killiany atlas. A linear model was used to test for main effect of group and main effect of verbal memory on white matter tract FA, covarying for age and sex. For both sets of analyses, results were corrected for multiple comparisons using false discovery rate (FDR). Results A significant main effect of group on whole-brain average FA was observed, with patients displaying lower average FA compared to healthy controls (Patients=0.291, controls=0.300, p<0.05). Whole-brain white matter tract FA analysis revealed that there are widespread differences between controls and individuals with a FEP. Group most strongly predicted white matter tract FA differences between left caudal anterior cingulate and left lateral orbitofrontal (patients mean FA=0.302, controls mean FA=0.342), left hippocampus and right isthmus cingulate (patient mean FA= 0.217 controls mean FA= 0.318), and finally left lingual and left rostral anterior cingulate (patients mean FA=0.162, controls mean FA= 0.249. However, none survived correction for multiple comparisons. Further, there was no significant association between verbal memory and white matter tract FA in FEP or HC. Discussion Findings from this study suggest there are some significant differences in whole-brain average FA between individuals experiencing a FEP and healthy controls. However, when analyzing whole-brain tract FA, none of the connections survived corrections for multiple comparisons. These findings might be limited by the scanner resolution included in this study, which may not capture more subtle differences. Nonetheless, these results are consistent with a cross-sectional study comparing healthy individuals to chronic and first-episode patients suggesting that modest differences are present early in the disease and increase as the disease progresses. We suggest that future studies analyze white matter tract using a longitudinal design to identify disease progression.
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Bijyal, Sajan, Avinash Rajra, and Virta Chauhan. "A study of total mesorectal excision for rectal cancer-upfront and after neoadjuvant chemotherapy or chemoradiation." International Surgery Journal 9, no. 9 (August 26, 2022): 1591. http://dx.doi.org/10.18203/2349-2902.isj20222236.
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Background: Colorectal cancer is a major cause of morbidity and mortality throughout the world. The incidence of rectal carcinoma is increasing in developing countries. Challenge in preoperative evaluation remains to identify patients who might benefit from neoadjuvant chemotherapy or chemoradiation (NACT/RT). The aim of this study was to study the correlation of final histopathology specimen (pathological tumor regression grade) with preoperative MRI (magnetic resonance tumor regression grade) (mrTRG).Method: This was a prospective observational analysis with operable rectal cancer of mid or distal rectum who underwent total mesorectal excision either upfront or following CT/RT. Preoperative MRI was done for local staging and also to assess response to neo-adjuvant CT/RT. Histo-pathological specimen was assessed for pathological tumor regression and correlation between mrTRG and pathological tumor regression grading (pTRG) analysed.Results: Out of 79 patients, 69 patients received NACRT and 10 patients underwent upfront surgery. On bivariate analysis mrTRG and pTRG corresponded in 47 patients whereas in 22 patients it didn’t correspond. There was a statistically significant agreement between mrTRG and pTRG (p=0.0491).Conclusions: mrTRG grading was identified as a reliable predictor of pathological response after neoadjuvant chemoradiotherapy and thus a predictor of completeness of resection. So, efforts should be routinely done to do a mrTRG grading as it not only provides oncological safety but also reduces post operative mortality.
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Tournier, J.-Donald, Robert Smith, David Raffelt, Rami Tabbara, Thijs Dhollander, Maximilian Pietsch, Daan Christiaens, Ben Jeurissen, Chun-Hung Yeh, and Alan Connelly. "MRtrix3: A fast, flexible and open software framework for medical image processing and visualisation." NeuroImage 202 (November 2019): 116137. http://dx.doi.org/10.1016/j.neuroimage.2019.116137.
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Yoo, Gyu Sang, Hee Chul Park, Jeong Il Yu, Doo Ho Choi, Won Kyung Cho, Young Suk Park, Joon Oh Park, et al. "Carcinoembryonic Antigen Improves the Performance of Magnetic Resonance Imaging in the Prediction of Pathologic Response after Neoadjuvant Chemoradiation for Patients with Rectal Cancer." Cancer Research and Treatment 52, no. 2 (April 15, 2020): 446–54. http://dx.doi.org/10.4143/crt.2019.261.
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PurposeThe purpose of this study was to investigate the role of carcinoembryonic antigen (CEA) levels in improving the performance of magnetic resonance imaging (MRI) for the prediction of pathologic response after the neoadjuvant chemoradiation (NCRT) for patients with rectalcancer.Materials and MethodsWe retrospectively reviewed the medical records of 524 rectal cancer patients who underwent NCRT and total mesorectal excision between January 2009 and December 2014. The performances of MRI with or without CEA parameters (initial CEA and CEA dynamics) for prediction of pathologic tumor response grade (pTRG) were compared by receiver-operating characteristic analysis with DeLong’s method. Cox regression was used to identify the independent factors associated to pTRG and disease-free survival (DFS) after NCRT.ResultsThe median follow-up was 64.0 months (range, 3.0 to 113.0 months). On multivariate analysis, poor tumor regression grade on MRI (mrTRG; p < 0.001), initial CEA (p < 0.001) and the mesorectal fascia involvement on MRI before NCRT (mrMFI; p=0.054) showed association with poor pTRG. The mrTRG plus CEA parameters showed significantly improved performances in the prediction of pTRG than mrTRG alone. All of mrTRG, mrMFI, and initial CEA were also identified as independent factors associated with DFS. The initial CEA further discriminated DFS in the subgroups with good mrTRG or that without mrMFI.ConclusionThe CEA parameters significantly improved the performance of MRI in the prediction of pTRG after NCRT for patients with rectal cancer. The DFS was further discriminated by initial CEA level in the groups with favorable MRI parameters.
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Jameson, Michael B., Kirsten Gormly, David Espinoza, Wendy Hague, Grahame Mark Jeffery, Timothy Jay Price, Christos Stelios Karapetis, et al. "SPAR: A randomized placebo-controlled phase 2 trial of simvastatin in addition to standard chemotherapy and radiation in preoperative treatment for rectal cancer: An AGITG clinical trial." Journal of Clinical Oncology 40, no. 16_suppl (June 1, 2022): TPS3646. http://dx.doi.org/10.1200/jco.2022.40.16_suppl.tps3646.
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TPS3646 Background: In retrospective studies statin use during preoperative chemo-radiation (pCRT) for rectal cancer is associated with improved overall survival, pathological tumor response and treatment toxicity. In vivo preclinical studies show that statins radiosensitize cancer cells, with improved tumor control and reduced radiation-induced gastrointestinal (GI) and skin toxicities. A prospective randomized trial is justified to confirm these clinically important benefits. Tumor regression following pCRT has strong prognostic significance, as assessed radiologically (MRI-based tumor regression grading [mrTRG]) prior to, or pathologically (pathTRG) following, surgery. Using mrTRG after each treatment phase in total neoadjuvant therapy (TNT) programs could assess incremental tumor regression and optimize patient management including surgery. Methods: Design: This double-blind phase 2 trial is recruiting 222 patients planned to receive long-course fluoropyrimidine-based pCRT for rectal adenocarcinoma at 17 sites in New Zealand and Australia. Patients are randomized to simvastatin 40mg or placebo daily for 90 days, starting 1 week prior to pCRT, with minimization for major prognostic variables. Pelvic MRI at baseline and 6-8 weeks after pCRT will assess mrTRG. A protocol amendment allows TNT using consolidation chemotherapy after pCRT; MRI timing is unchanged. Primary objective: comparison of rates of grades 1-2 mrTRG following pCRT with simvastatin or placebo, considering mrTRG in 4 ordered categories (1, 2, 3, 4-5). Secondary objectives: comparison of rates of grades 1-2 pathTRG in resected tumors; incidence of > grade 2 acute GI and non-GI adverse events (AE); incidence of late GI AE; compliance with intended pCRT and trial medication; proportion of patients undergoing surgical resection post-pCRT; 3-year local recurrence rate, disease-free and cancer-specific survival; and pathological scores for radiation colitis. Tertiary and correlative objectives: association between mrTRG and pathTRG grouping; inter-observer scoring agreement on mrTRG and pathTRG; comparison of the association between tumor CD3+ and/or CD8+ T-cell infiltrates in diagnostic biopsies and pathTRG; intensity and distribution of subsets of infiltrating T-cells in irradiated resected normal and malignant tissue; and the effect of simvastatin on markers of systemic inflammation (modified Glasgow prognostic score and the neutrophil-lymphocyte ratio). Eligibility criteria exclude statin use within 6 weeks prior to trial entry, patients intolerant of statins, and planned use of oxaliplatin or biological agents during pCRT. Trial recruitment commenced April 2018 and 95 of 222 patients have been recruited as at 21 January 2022. Clinical trial information: ACTRN12617001087347.
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Žitko, Urban. "Do konca aprila že 31 nezgod in 7 mrtvih." InfoGozd : skrbno z gozdom 3, no. 5 (2022): 18–24. http://dx.doi.org/10.20315/ig.2022.0027.
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Berezovskaia, T. P., Ya A. Dayneko, A. A. Nevolskikh, S. A. Ivanov, and A. D. Kaprin. "Prospective Assessment of mrTRG System Used for Determining the Efficiency of Neoadjuvant Chemoradiotherapy in Patients with Rectal Cancer." Journal of radiology and nuclear medicine 102, no. 1 (March 10, 2021): 6–17. http://dx.doi.org/10.20862/0042-4676-2021-102-1-6-17.
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Background. Evaluation of the therapeutic effect of neoadjuvant chemoradiotherapy (nCRT) for rectal cancer (RC) is of prognostic value and allows to individually plan the type and extent of further surgical intervention. One of the most promising methods of MRI evaluation is magnetic resonance tumour regression grade (mrTRG) system. However, the reliability and safety of this method must be confirmed by the results of clinical trials and practical application.Objective: to analyze our experience in the prospective use of mrTRG system and evaluate the additional contribution of diffusion-weighted images (DWI) to its diagnostic efficiency.Material and methods. The mrTRG values were determined in 125 (including 30 with DWI) patients with locally advanced RC who received combined treatment with nCRT in Tsyb Medical Radiological Research Centre from 2015 to 2019. The obtained data were compared with the modified pathologic response based on Lushnikov’s definition, and the diagnostic parameters of differentiation of patients responding and nonresponding to nCRT were calculated.Results. When the mrTRG system was used without DWI, the following sensitivity and specificity values as well as positive and negative predictive values were obtained: 75%, 60%, 70% and 67%, respectively, with DWI – 87%, 87%, 87% and 87%, respectively.Conclusion. A prospective evaluation of the result of nCRT using the mrTRG system has moderate accuracy for stratification of patients responding and non-responding to nCRT, which requires the use of additional criteria for MRI scores to select patients who may receive less aggressive surgical treatment. Despite the small number of patients with DWI, we obtained higher values of the diagnostic parameters. In this regard, we consider it appropriate to use DWI when assessing the treatment response.
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Munier, Sean M., Akshay N. Desai, Nitesh V. Patel, and Shabbar F. Danish. "Effects of Intraoperative Magnetic Resonance Thermal Imaging Signal Artifact During Laser Interstitial Thermal Therapy on Thermal Damage Estimate and Postoperative Magnetic Resonance Imaging Ablative Area Concordance." Operative Neurosurgery 18, no. 5 (July 17, 2019): 524–30. http://dx.doi.org/10.1093/ons/opz182.
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Abstract BACKGROUND Magnetic resonance-guided laser interstitial thermal therapy (MRgLITT) is a minimally invasive procedure that utilizes intraoperative magnetic resonance thermal imaging (MRTI) to generate a thermal damage estimate (TDE) of the ablative area. In select cases, the MRTI contains a signal artifact or defect that distorts the ablative region. No study has considered the impact of this artifact on TDE accuracy. OBJECTIVE To determine the effect of intraoperative MRTI signal artifact on postoperative magnetic resonance imaging (MRI)-predicted ablative area. METHODS All ablations were performed using the Visualase MRI-Guided Laser Ablation System (Medtronic). Patients were grouped based on whether the intraoperative MRTI contained signal artifact that distorted the ablative region. Cross-sectional area of the ablative lesion from the MRI image was measured, and the difference between intraoperative TDE and postoperative MRI cross-sectional area was calculated and compared between groups with and without intraoperative MRTI artifact. RESULTS A total of 91 patients undergoing MRgLITT for various surgical indications were examined. MRTI artifact was observed in 43.9% of cases overall. The mean absolute difference between TDE and the postoperative MRI cross-sectional area was 94.8 mm2 (SEM = 11.6) in the group with intraoperative MRTI artifact and 54.4 mm2 (SEM = 5.5) in the nonartifact group. CONCLUSION MRTI signal artifact is common during LITT. The presence of signal artifact during intraoperative MRTI results in higher variation between intraoperative TDE and postoperative MRI cross-sectional ablative area. In cases in which intraoperative MRTI artifact is observed, there may be a larger degree of variation between observed intraoperative TDE and measured postoperative MRTI ablative area.
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Minea, Marius, and Iulian Bădescu. "Delay Factors Modelling for Real-Time Traffic Information Systems." WSEAS TRANSACTIONS ON COMMUNICATIONS 20 (December 28, 2021): 192–97. http://dx.doi.org/10.37394/23204.2021.20.25.
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The paper is presenting an overview of the factors that lead to critical delays which may occur in multi-modal realtime traffic and travel information systems (MRTTI) employing cellular data network communications. The analysis is taking into account MRTTI systems that use mobile devices to detect position and speed of a vehicle and receive traffic information furthermore used to adjust a previously defined route from origin to destination points. Information transmission delays are also investigated for route guidance systems used in critical applications, such as emergency vehicles management. The identification of the delay factors and some models regarding the delays are presented along with measurements on a real system.
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Jakopović, Željko, and Iva Čanak. "Usporedba vezanja AFM1 iz mlijeka živim, mrtvim i liofiliziranim stanicama BMK." Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam 13, no. 1-2 (September 3, 2018): 32–37. http://dx.doi.org/10.31895/hcptbn.13.1-2.3.
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Mikotoksini su sekundarni metaboliti niske molekulske mase koje sintetiziraju plijesni iz rodova Aspergillus, Penicillium i Fusarium te su česti kontaminanti raznovrsnih prehrambenih proizvoda. Kako bi se smanjio rast plijesni, a time i udio mikotoksina u namirnicama, koriste se razne fizikalne i kemijske metode. Posljednjih nekoliko godina ispituje se učinak bakterija mliječne kiseline (BMK) na sposobnost inhibicije rasta plijesni i njihova uloga u uklanjanju mikotoksina iz kontaminirane hrane. Zbog svojih, po zdravlje korisnih svojstava, bakterije mliječne kiseline predstavljaju izvrsno rješenje za tretiranje namirnica kontaminiranih mikotoksinima. Cilj ovog rada bio je ispitati i usporediti sposobnosti, živih, mrtvih i liofiliziranih stanica BMK na vezanje aflatoksina M1 (AFM1) u umjetno kontaminiranom mlijeku. Ovisno o korištenom soju, vremenu inkubacije i tretmanu, uspješnost vezanja AFM1 stanicama BMK iznosila je 23,73-94,49%. Liofilizirane stanice ispitivanih sojeva BMK manje (p<0,05) vežu AFM1 u mlijeku, a jedini izuzetak su stanice Lactobacillus rhamnosus KM koje nakon 4 sata vežu AFM1 u najvećem postotku (>90%). Najveća razlika u uspješnosti vezanja AFM1 između mrtvih i liofiliziranih stanica uočljiva je kod soja L. rhamnosus KM i iznosi 62,49% dok su razlike u vezanju AFM1 najmanje kod Lactococcus lactis 5MS1. Koncentracija slobodnog AFM1 u mlijeku nakon tretiranja BMK određivana je visoko djelotvornom tekućinskom kromatografijom (HPLC).
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Yang, Menghua, Jennifer L. Giel, Tao Cai, Zengtao Zhong, and Jun Zhu. "The LuxR Family Quorum-Sensing Activator MrtR Requires Its Cognate Autoinducer for Dimerization and Activation but Not for Protein Folding." Journal of Bacteriology 191, no. 1 (October 31, 2008): 434–38. http://dx.doi.org/10.1128/jb.01247-08.
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ABSTRACT MrtR, a LuxR homolog in Mesorhizobium tianshanense, is important for symbiosis. We found that MrtR requires its cognate N-acylhomoserine lactone for forming dimers, binding to a single DNA site and activating the downstream promoter. However, MrtR is able to fold independently of its ligand.
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Kaske, M., and W. V. Engelhardt. "The effect of size and density on mean retention time of particles in the gastrointestinal tract of sheep." British Journal of Nutrition 63, no. 3 (May 1990): 457–65. http://dx.doi.org/10.1079/bjn19900133.
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The selective retention of particles in the reticulo-rumen and in the gastrointestinal tract distal to the reticulo-rumen was studied in fistulated sheep maintained on a roughage diet. Polyethylene glycol and plastic particles of different lengths (1 and 10 mm) and densities (092, 1.03, 1.22 and 1.44 g/ml) were either fed or were introduced into the omasum. The mean retention time in the reticulo-rumen (MRTRR) of 1 mm long particles with a density of approximately 1.0 g/ml was about 67 h, that is eight times longer than the MRTRR of fluid; the heavier particles were retained only three times longer than fluid. Particles with a length of 10 mm were retained in the reticulo-rumen 19–28 h longer than 1 mm long particles of the same density. Particles with a length of 10 mm were reduced to smaller particles (0.5–4 mm) due to rumination. Multiple regression analysis indicated that particle density and particle size accounted for 59 and 28% of the total variation of MRTRR respectively. The mean retention time distal to the reticulo- rumen (MRTGut) of 1 and 10 mm long particles with a density near 1.0 g/ml was 18–19 h, similar to that of fluid (16 h). The heavier particles were retained about 3–8 h longer.
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Brown, Gina, Uday Bharat Patel, Jesus Santos Cores, Maria Isabel Gil Garcia, Juan Ramón Ayuso, Iciar Puchades Roman, Fernando Mas Estelles, et al. "Comparison of magnetic resonance imaging and histopathological response to neoadjuvant chemotherapy in locally advanced rectal cancer: The GEMCAD 0801 trial." Journal of Clinical Oncology 30, no. 15_suppl (May 20, 2012): e14097-e14097. http://dx.doi.org/10.1200/jco.2012.30.15_suppl.e14097.
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e14097 Background: MRI methods for rectal cancer response assessment post chemoradiotherapy include post treatment T staging(ymrT), tumor regression grading(mrTRG) and length change/modified RECIST measurement. The usefulness of MRI in evaluating response to neoadjuvant chemotherapy has not been investigated. We assessed the reproducibility and agreement of these three parameters with histopathological T and TRG stage(ypT, pTRG). Methods: 28 eligible patients were enrolled in a prospective phase II trial to evaluate safety and efficacy of neoadjuvant CAPOX-B in patients with MRI defined T3 rectal adenocarcinoma. Patients received 4 cycles of Cap 2000 mg/m2(d1-14),Ox 130 mg/m2(d1) and B 7.5 mg/kg(d1) every 3 weeks(last cycle without B). Seven radiologists assessed MRIs using the following categories: ymrT (T0-T4 using T3 substaging), mrTRG (1-5), and length change(Stable disease, Complete response,Partial response). Agreement was assessed by kappa (central reviewer data verses each local centre reviewer).Agreement between central reviewer MRI results and both pathology endpoints was also assessed. Results: 24 patients had evaluable pre and post chemotherapy imaging and pathology (4 did not have post treatment MRI). Thirteen patients had good response (ypT0-3a) and 11 had poor response (>ypT3a). Sixteen patients had good pTRG(2-4) and 8 had poor pTRG(0&1). ymrTRG showed a moderate level of reproducibility;K=0.45-0.58. ymrT showed a fair to moderate level of agreement;K=0.2-0.53. Length assessment also showed a fair level of agreement; K=0.21-0.38. ymrTRG showed 75% agreement with ypT(16/22); K=0.49(0.13-0.84) and 79% agreement with pTRG(19/24); K=0.55(0.22-0.88). ymrT showed a fair level of agreement with ypT;K=0.21, pTRG;K=0.2, length assessment showed slight agreement with ypT;K=0.1, pTRG;K=0.1. Conclusions: This is the first study to show MRI can evaluate response of rectal cancer following neoadjuvant chemotherapy. As mrTRG showed best agreement with pathology, we recommend mrTRG as the preferred method of post treatment assessment in this setting.
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Munier, Sean M., Allison S. Liang, Akshay N. Desai, Jose K. James, and Shabbar F. Danish. "Characterization of Magnetic Resonance Thermal Imaging Signal Artifact During Magnetic Resonance Guided Laser-Induced Thermal Therapy." Operative Neurosurgery 19, no. 5 (July 31, 2020): 619–24. http://dx.doi.org/10.1093/ons/opaa229.
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Abstract BACKGROUND Magnetic resonance-guided laser interstitial thermal therapy (MRgLITT) is a minimally invasive procedure that utilizes intraoperative magnetic resonance thermal imaging (MRTI) to generate a thermal damage estimate (TDE) of the ablative area. In select cases, the MRTI contains a signal artifact or defect that distorts the ablative region. No study has attempted to characterize this artifact. OBJECTIVE To characterize MRTI signal the artifact in select cases to better understand its potential relevance and impact on the ablation procedure. METHODS All ablations were performed using the Visualase magnetic resonance imaging-guided laser ablation system (Medtronic). Patients were included if the MRTI contained signal artifact that distorted the ablative region during the first thermal dose delivered. Ablation artifact was quantified using MATLAB version R2018a (Mathworks Inc, Natick, Massachusetts). RESULTS A total of 116 patients undergoing MRgLITT for various surgical indications were examined. MRTI artifact was observed in 37.0% of cases overall. Incidence of artifact was greater at higher powers (P < .001) and with longer ablation times (P = .024), though artifact size did not correlate with laser power or ablation duration. CONCLUSION MRTI signal artifact is common during LITT. Higher powers and longer ablation times result in greater incidence of ablation artifact, though artifact size is not correlated with power or duration. Future studies should aim to evaluate effects of artifact on postoperative imaging and, most notably, patient outcomes.
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Cho, Eunhae, Sung Woo Jung, In Ja Park, Jong Keon Jang, Seong Ho Park, Seung-Mo Hong, Jong Lyul Lee, et al. "Improvement in the Assessment of Response to Preoperative Chemoradiotherapy for Rectal Cancer Using Magnetic Resonance Imaging and a Multigene Biomarker." Cancers 13, no. 14 (July 12, 2021): 3480. http://dx.doi.org/10.3390/cancers13143480.
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The response to preoperative chemoradiotherapy (PCRT) is correlated with oncologic outcomes in patients with locally advanced rectal cancer. Accurate prediction of PCRT response before surgery can provide crucial information to aid clinicians in further treatment planning. This study aimed to develop an evaluation tool incorporating a genetic biomarker and magnetic resonance imaging (MRI) to improve the assessment of response in post-CRT patients with locally advanced rectal cancer. A total of 198 patients who underwent PCRT followed by surgical resection for locally advanced rectal cancer between 2010 and 2016 were included in this study. Each patient’s response prediction index (RPI) score, a multigene biomarker developed in our previous study, and magnetic resonance tumor regression grade (mrTRG) score were added to create a new predictive value for pathologic response after PCRT, called the combined radiation prediction value (cRPV). Based on the new value, 121 and 77 patients were predicted to be good and poor responders, respectively, showing significantly different cRPV values (p = 0.001). With an overall predictive accuracy of 84.8%, cRPV was superior to mrTRG and RPI for the prediction of pathologic chemoradiotherapy response (mrTRG, 69.2%; RPI, 77.3%). In multivariate analysis, cRPV was found to be the sole predictor of tumor response (odds ratio, 32.211; 95% confidence interval, 14.408–72.011; p = 0.001). With its good predictive value for final pathologic regression, cRPV may be a valuable tool for assessing the response to PCRT before surgery.
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De Landro, Martina, Jacopo Ianniello, Maxime Yon, Alexey Wolf, Bruno Quesson, Emiliano Schena, and Paola Saccomandi. "Fiber Bragg Grating Sensors for Performance Evaluation of Fast Magnetic Resonance Thermometry on Synthetic Phantom." Sensors 20, no. 22 (November 12, 2020): 6468. http://dx.doi.org/10.3390/s20226468.
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The increasing recognition of minimally invasive thermal treatment of tumors motivate the development of accurate thermometry approaches for guaranteeing the therapeutic efficacy and safety. Magnetic Resonance Thermometry Imaging (MRTI) is nowadays considered the gold-standard in thermometry for tumor thermal therapy, and assessment of its performances is required for clinical applications. This study evaluates the accuracy of fast MRTI on a synthetic phantom, using dense ultra-short Fiber Bragg Grating (FBG) array, as a reference. Fast MRTI is achieved with a multi-slice gradient-echo echo-planar imaging (GRE-EPI) sequence, allowing monitoring the temperature increase induced with a 980 nm laser source. The temperature distributions measured with 1 mm-spatial resolution with both FBGs and MRTI were compared. The root mean squared error (RMSE) value obtained by comparing temperature profiles showed a maximum error of 1.2 °C. The Bland-Altman analysis revealed a mean of difference of 0.1 °C and limits of agreement 1.5/−1.3 °C. FBG sensors allowed to extensively assess the performances of the GRE-EPI sequence, in addition to the information on the MRTI precision estimated by considering the signal-to-noise ratio of the images (0.4 °C). Overall, the results obtained for the GRE-EPI fully satisfy the accuracy (~2 °C) required for proper temperature monitoring during thermal therapies.
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Virant, Špela. "Ne živi ne mrtvi: sodobna nemška dramatika in množični mediji." Ars & Humanitas 5, no. 1 (July 25, 2011): 71–83. http://dx.doi.org/10.4312/ars.5.1.71-83.
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Virant, Špela. "Ne živi ne mrtvi: sodobna nemška dramatika in množični mediji." Ars & Humanitas 5, no. 1 (July 25, 2011): 71–83. http://dx.doi.org/10.4312/ah.5.1.71-83.
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Danilović, Aleksandar. "Faith in ressurrection of the dead and creatio ex nihilo in 2 Macc 7." Sabornost, no. 14 (2020): 39–54. http://dx.doi.org/10.5937/sabornost2014039d.
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The narrative of the martyrdom of a mother and seven brothers has had an enormous impact on the history of both Church and Synagogue. The cult of the Maccabean martyrs began to develop very early, so that in the fourth century at the latest, they were celebrated among the Christians. The story about them is to be found in the seventh chapter of the Second Book of Maccabees. Its canonical status has become the subject of debate in the last few centuries, both among the Orthodox and other Christians. The Synagogue rejected this book in its entirety, although it contains the oldest recorded testimony about the celebration of Hanukkah, one of the most important Jewish holidays today. However, the story of the mother and brothers remained preserved in Talmud in a certain form. Apart from the story of martyrdom, the seventh chapter hides one of the oldest, and certainly one of the most vivid descriptions of the early faith in the resurrection from the dead. In addition, the seventh chapter reveals the oldest explicitly expressed faith in God, who is the absolute Creator and who creates the world out of nothing. This idea will later become one of the central motives of Christian and Jewish cosmology.
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Patel, Uday B., Fiona Taylor, Lennart Blomqvist, Christopher George, Hywel Evans, Paris Tekkis, Philip Quirke, et al. "Magnetic Resonance Imaging–Detected Tumor Response for Locally Advanced Rectal Cancer Predicts Survival Outcomes: MERCURY Experience." Journal of Clinical Oncology 29, no. 28 (October 1, 2011): 3753–60. http://dx.doi.org/10.1200/jco.2011.34.9068.
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Purpose To assess magnetic resonance imaging (MRI) and pathologic staging after neoadjuvant therapy for rectal cancer in a prospectively enrolled, multicenter study. Methods In a prospective cohort study, 111 patients who had rectal cancer treated by neoadjuvant therapy were assessed for response by MRI and pathology staging by T, N and circumferential resection margin (CRM) status. Tumor regression grade (TRG) was also assessed by MRI. Overall survival (OS) was estimated by using the Kaplan-Meier product-limit method, and Cox proportional hazards models were used to determine associations between staging of good and poor responders on MRI or pathology and survival outcomes after controlling for patient characteristics. Results On multivariate analysis, the MRI-assessed TRG (mrTRG) hazard ratios (HRs) were independently significant for survival (HR, 4.40; 95% CI, 1.65 to 11.7) and disease-free survival (DFS; HR, 3.28; 95% CI, 1.22 to 8.80). Five-year survival for poor mrTRG was 27% versus 72% (P = .001), and DFS for poor mrTRG was 31% versus 64% (P = .007). Preoperative MRI-predicted CRM independently predicted local recurrence (LR; HR, 4.25; 95% CI, 1.45 to 12.51). Five-year survival for poor post-treatment pathologic T stage (ypT) was 39% versus 76% (P = .001); DFS for the same was 38% versus 84% (P = .001); and LR for the same was 27% versus 6% (P = .018). The 5-year survival for involved pCRM was 30% versus 59% (P = .001); DFS, 28 versus 62% (P = .02); and LR, 56% versus 10% (P = .001). Pathology node status did not predict outcomes. Conclusion MRI assessment of TRG and CRM are imaging markers that predict survival outcomes for good and poor responders and provide an opportunity for the multidisciplinary team to offer additional treatment options before planning definitive surgery. Postoperative histopathology assessment of ypT and CRM but not post-treatment N status were important postsurgical predictors of outcome.
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ISPÁNOVICS CSAPÓ, Julianna. "ELEMENTI EVROPSKE KULTURE U TEKSTOVIMA DANILA KIŠA I OTOA TOLNAIJA Enciklopedija mrtvih, Svetionik na prodaju." Hungarológiai Közlemények 16, no. 1 (July 15, 2015): 58. http://dx.doi.org/10.19090/hk.2015.1.58-71.
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A tanulmány köztes helyzetben generálódó szövegeket, Danilo Kiš elbeszélésfüzérét és Tolnai Ottó festettvíz-prózáját vizsgálja. A műfaji képlékenység, színjátszás intertextuális vonatkozásai család- és társadalmi mitológiák nyomvonalán, európai kultúrminták (térkép, családfa, enciklopédia, azúr, halál, Halottak Könyve, Necronomicon) felhasználásával bontja ki a textusokban megjelenő régiók kulturális irodalmi térképét. A térképen megjelenő hangsúlyos elemek (áramlás, torkolat, tenger) egyetlen végpontban találkoznak, a szövegeket generáló vezérmotívum, a (kék) halál szférájában. A kutatás célja a fenti szövegek elemzése, a két textus közti intertextuális kapcsolatok kibontása, különös tekintettel az alkalmazott európai kultúrmintákra.
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Behm, David G., and Digby G. Sale. "Voluntary and Evoked Muscle Contractile Characteristics in Active Men and Women." Canadian Journal of Applied Physiology 19, no. 3 (September 1, 1994): 253–65. http://dx.doi.org/10.1139/h94-021.
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Eight men and 8 women were tested for voluntary and isometric evoked contractions of the dorsiflexors. Maximal voluntary contractions were conducted isometrically (30° plantarflexion) and isokinetically. Men produced greater peak torques at all but the highest isokinetic velocity. They exceeded women in measures of absolute voluntary isometric (33.8%), tetanic (46.3%), and twitch (37.9%) peak torque. Men demonstrated a higher tetanus/MVC ratio (17.8%). This ratio disparity would indicate that gender differences in peak torque in this study were more dependent upon muscle mechanisms. Although men exceeded women in measures of absolute voluntary maximum rate of torque development (MRTD) (38.7%), tetanic MRTD (39.1%), twitch MRTD (27.9%), voluntary maximum rate of torque relaxation (MRTR) (46.9%), and tetanic MRTR (41.9%), there were no significant gender differences in percentage rates of torque development or relaxation with voluntary or evoked contractions. Therefore the central and peripheral mechanisms controlling MRTD and MRTR may be similar between the sexes. Key words: gender strength differences, muscle contraction, rate of torque, tetanus, twitch
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Gulay, Kevin Christian M., Edgar Esparza, Xinlian Zhang, Evangeline S. Mose, Jonathan Weitz, Minya Pu, Karen Messer, Andrew M. Lowy, and Herve Tiriac. "Abstract A072: Strategies to improve KRAS G12D inhibitor (MRTX1133) therapy in pancreatic ductal adenocarcinoma." Cancer Research 82, no. 22_Supplement (November 15, 2022): A072. http://dx.doi.org/10.1158/1538-7445.panca22-a072.
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Abstract Background: More than 90% of pancreatic ductal adenocarcinomas (PDAC) harbor oncogenic KRAS, with KRAS G12D being the predominant mutation. Recently, a novel KRAS G12D inhibitor, MRTX-1133, has been developed, potentially revolutionizing PDAC treatment. However, clinical studies on KRAS G12C inhibitors suggest that there may be intrinsic resistance to KRAS inhibitors (KRASi) and acquired resistance can develop rapidly in patients. Moreover, our preliminary data suggest that acquired resistance mechanisms to chemotherapy can also confer partial resistance to KRASi. We hypothesize that effective killing of tumor cells with KRASi will require a rationally designed combinatorial treatment. Methods: We tested chemotherapy and targeted pathway inhibitors which can inhibit signaling upstream and downstream of KRAS to identify drugs that synergize with MRTX-1133. We treated resected human PDAC and mouse tumors from Kras-G12D; P53-R172H; PDX-Cre (KPC) prepared as organotypic slice cultures to test our identified drug combinations. We orthotopically inoculated KPC PDAC cells and treated mice with vehicle, single-agent drugs (MRTX1133 or Afatinib), or combination therapy. We established MRTX-1133-resistant (MRTXR) models in human SUIT2 cells, human organoids and mouse KPC cell lines to study the mechanisms of KRASi resistance. We performed RNA-seq and Reverse Phase Protein Array in these resistance models to identify pathways differentially activated during resistance development. Results: We found that irreversible ErbB inhibitors, Afatinib and Neratinib, had the greatest synergy with MRTX-1133 in parental and in MRTXR cells. We found that combination therapy with MRTX-1133 and Afatinib decreased the number of proliferating cells compared to the control or single-agent treatment in the human and mouse slice cultures. Combination therapy with MRTX1133 and Afatinib significantly reduced tumor growth in mice compared to the vehicle or single-agent controls. We also found that resistant cells demonstrated increased EGFR and HER2 phosphorylation compared to parental cells and that the MRTX-1133 and Afatinib drug combo synergistically decreased EGFR and HER2 phosphorylation. We identified and mapped the differentially expressed pathways and genes involved in KRASi resistance using RNA-seq and RPPA analyses. Conclusion: We identified a likely pathway of resistance to novel KRAS G12D inhibitors, and we demonstrate that this resistance can be effectively targeted using existing therapeutics targeting the ErbB pathway. Citation Format: Kevin Christian M. Gulay, Edgar Esparza, Xinlian Zhang, Evangeline S. Mose, Jonathan Weitz, Minya Pu, Karen Messer, Andrew M. Lowy, Herve Tiriac. Strategies to improve KRAS G12D inhibitor (MRTX1133) therapy in pancreatic ductal adenocarcinoma [abstract]. In: Proceedings of the AACR Special Conference on Pancreatic Cancer; 2022 Sep 13-16; Boston, MA. Philadelphia (PA): AACR; Cancer Res 2022;82(22 Suppl):Abstract nr A072.
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Miščin, Daniel. "Ontološki ključ razumijevanja slike Mrtvi Krist u grobu Hansa Holbeina mlađeg." Crkva u svijetu 56, no. 4 (December 15, 2021): 635–54. http://dx.doi.org/10.34075/cs.56.4.5.
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Prihvaćajući argumente u prilog tzv. kartuzijanskoj hipotezi, tj. stajalištu da je Holbeinova slika Mrtvi Krist u grobu izvorno zamišljena kao nadgrobni spomenik u baselskoj kartuziji, autor analizira tekst epitafa s kojim je ona trebala činiti cjelinu. Kao rezultat te analize, autor naglašava važnost ontološkog značenjskog sloja tog epitafa u čijem svjetlu razvija ontološki ključ za razumijevanje te slike, povezujući ga sa smislom tradicionalne kartuzijanske opomene memento mori. Uzimajući u obzir ontološki smisao te opomene, tjeskoba promatrača Holbeinova Mrtvog Krista tumači se na tragu refleksije o pojmu Ništa (das Nichts) u egzistencijalnoj analici Martina Heideggera i Bernharda Weltea.
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Grishko, P. Yu, A. V. Mishchenko, O. V. Ivko, D. V. Samsonov, and A. M. Karachun. "Treatment monitoring of locally advanced rectal cancer based on multiparametric magnetic resonance tomography." Pelvic Surgery and Oncology 10, no. 1 (August 29, 2020): 20–27. http://dx.doi.org/10.17650/2686-9594-2020-10-1-20-27.
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Objective: to determine the predicting factors for the effectiveness of neoadjuvant treatment in colorectal cancer based on the analysis of overall and relapse-free survival, as well as the possibility of multiparametric magnetic resonance imaging (MRI) in stratifying patients into groups with favorable and unfavorable clinical course.Materials and methods. 112 patients who received preoperative chemoradiotherapy (n = 85) and chemoradiotherapy supplemented with neoadjuvant polychemotherapy (n = 27) followed by surgery were enrolled in retrospective study. To determine the most significant predicting factors and criteria for evaluating the effectiveness of treatment that affect overall and relapse-free survival, Kaplan–Meier estimator and Cox regression were used.Results. The relapse-free survival was significantly affected by the presence or absence of extramural venous invasion according to MRI (mrEMVI) (p = 0.0001), circumferential resection margin status according to pathomorphological data (pCRM) (p = 0.031), change in volume of tumor (mrVolumetric analysis) (p = 0.015), tumor regression grade according to MRI (mrTRG) (p = 0.017) and pathomorphological data (pTRG) (p = 0.038). Independent predictors of overall survival were: extramural venous invasion according to MRI (mrEMVI) (p = 0.0001), posttreatment N staging (p = 0.047) and tumor regression grade according to MRI (mrTRG) (p = 0.059). Based on the most significant MR criteria, a mathematical model was developed to predict the risk of relapse after neoadjuvant treatment.Conclusions. MRI allows stratifying patients into groups with a favorable and unfavorable prognosis at the preoperative stage and optimizing the management of patients after surgery taking into account pathomorphological data.
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Oyefiade, Adeoye, Kiran Beera, Iska Moxon-Emre, Jovanka Skocic, Ute Bartels, Suzanne Laughlin, Vijay Ramaswamy, and Donald Mabbott. "QOL-09. WHOLE-BRAIN WHITE MATTER NETWORK CONNECTIVITY IS DISRUPTED BY PEDIATRIC BRAIN TUMOR TREATMENT." Neuro-Oncology 22, Supplement_3 (December 1, 2020): iii432. http://dx.doi.org/10.1093/neuonc/noaa222.673.
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Abstract INTRODUCTION Treatments for pediatric brain tumors (PBT) are neurotoxic and lead to long-term deficits that are driven by the perturbation of underlying white matter (WM). It is unclear if and how treatment may impair WM connectivity across the entire brain. METHODS Magnetic resonance images from 41 PBT survivors (mean age: 13.19 years, 53% M) and 41 typically developing (TD) children (mean age: 13.32 years, 51% M) were analyzed. Image reconstruction, segmentation, and node parcellation were completed in FreeSurfer. DTI maps and probabilistic streamline generation were completed in MRtrix3. Connectivity matrices were based on the number of streamlines connecting two nodes and the mean DTI (FA) index across streamlines. We used graph theoretical analyses to define structural differences between groups, and random forest (RF) analyses to identify hubs that reliably classify PBT and TD children. RESULTS For survivors treated with radiation, betweeness centrality was greater in the left insular (p < 0.000) but smaller in the right pallidum (p < 0.05). For survivors treated without radiation (surgery-only), betweeness centrality was smaller in the right interparietal sulcus (p < 0.05). RF analyses showed that differences in WM connectivity from the right pallidum to other parts of the brain reliably classified PBT survivors from TD children (classification accuracy = 77%). CONCLUSIONS The left insular, right pallidum, and right inter-parietal sulcus are structurally perturbed hubs in PBT survivors. WM connectivity from the right pallidum is vulnerable to the long-term effects of treatment for PBT.
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Ricchiuti, Vittorio, and Pietro Ciammaichella. "Analysis of output slave clock stability in SDH systems in terms of percentile MRTIE(τ)." International Journal of Communication Systems 11, no. 6 (November 1998): 351–57. http://dx.doi.org/10.1002/(sici)1099-1131(199811/12)11:6<351::aid-dac376>3.0.co;2-#.
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Milosavljević, Mladen, Dušica Milosavljević, and Sunčica Milosavljević. "Forenzičko – kriminalistički aspekti identifikacije (tragova) kostura (leševa) u segmentu forenzičke bioantropologije." Годишњак факултета правних наука - АПЕИРОН 1, no. 1 (July 15, 2011): 117. http://dx.doi.org/10.7251/gfp1101117m.
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Borba sa organiziranim kriminalom, te slučajevi ubijenih žrtava i neophodnost njihove identifikacije, u kasnijim postupcima pred istražne i ekspertne organe postavljaju veoma teške zadatke. Ovo tim prije, što kriminalci veoma često preduzimaju različite radnje u cilju što većeg uništenja/destrukcije ubijenih, a sve u cilju onemogućavanja pouzdane identifikacije. S druge strane, identifikacija umrlih/ubijenih može biti povezana i sa različitim terorističkim (pa i samoubilačkim) aktivnostima u kojima može poginuti veći broj nevinih civila. Opet, nemoguće je zaobići mogućnost masovnih nezgoda (npr. pad aviona, sudari vozova, autobusa i slično), gdje imamo situacije sa velikim brojem mrtvih u različitim stanjima (kompletna i nekompletna tijela). Središte rada biće, ipak, usmjereno ka identifikaciji kostura. U cjelokupnom segmentu veoma je važno razlikovati životinjske od ljudskih kostiju, utvrditi pol, starost i visinu žrtava/lica, a u konačnici izvršiti preciznu identifikaciju konkretnog lica. O navedenim parametrima upravo govorimo u ovom radu.
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Bender, Andrew, Sandra Duezel, Ulman Lindenberger, Ilja Demuth, Elisabeth Steinhagen-Thiessen, Thad Polk, and Simone Kühn. "PROXIES OF FRAILTY AND INFLAMMATION PREDICT ALTERED WHITE MATTER MICROSTRUCTURE IN OLDER ADULTS." Innovation in Aging 6, Supplement_1 (November 1, 2022): 291. http://dx.doi.org/10.1093/geroni/igac059.1156.
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Abstract Age-related elevations in inflammation are associated with both neurodegeneration and increased frailty in older adults. Here we used state-of-the-art diffusion-MRI (dMRI) methods to examine how specific markers of white matter (WM), including fiber density [FD], fiber cross-section [FC], and extracellular cerebral spinal fluid [CSF] are linked to inflammation and frailty in a population-based aging cohort. We hypothesized that increased inflammation and frailty would be associated with reduced FD and FC and increased CSF. Participants included 255 older adults (Mage=70.46; SD=3.79 years; 42% women) recruited from the Berlin Aging Study-II (BASE-II) for MRI scanning. Measures of blood serum ferritin and grip strength (corrected for sex and body mass) served as proxies of inflammation and frailty, respectively. Processing of dMRI data (b=1000 s/mm2; 60-directions) followed the MRtrix3 fixel-based analysis (FBA) pipeline, extended for voxelwise estimation of FD, FC, and CSF, with data transformed to a sample-specific template. The voxelwise estimates of FD, FC, and CSF were then regressed on the measures of blood serum ferritin and grip strength, correcting for multiple comparisons. Greater inflammation (ferritin) and frailty (grip strength) predicted lower FD in the internal capsule and anterior commissure. Higher ferritin also predicted lower FC in dorsal cingulum and forceps minor. Finally, frailty, but not inflammation, predicted increased CSF in multiple regions. These findings demonstrate common and specific associations of inflammation and frailty with WM in older adults and highlight the utility of newer methods for characterizing WM alterations.
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KUSABA, Kenichirou, Seiji FUJINO, and Takao HATAZAKI. "501 Performance estimation of MRTR method on PC with multicores." Proceedings of The Computational Mechanics Conference 2009.22 (2009): 66–67. http://dx.doi.org/10.1299/jsmecmd.2009.22.66.
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Takebe, Naoko, Thomas Dexheimer, Thomas Silvers, Rene Delosh, Julie Laudeman, Siddhartha Paul, Russell Reinhardt, et al. "Abstract 1054: RAS-pathway inhibitors (Sotorasib, MRTX-1257, TNO-155, BI-3406) in a Complex Spheroid Combination Screen with PDMR Cell Lines." Cancer Research 82, no. 12_Supplement (June 15, 2022): 1054. http://dx.doi.org/10.1158/1538-7445.am2022-1054.
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Abstract The KRAS G12C selective inhibitors, sotorasib and MRTX-1257, as well as the SHP-2 inhibitor TNO-155 and the SOS-1 inhibitor were assayed alone and in combination with approved and investigational anticancer agents in complex spheroids including tumor cells, endothelial cells (HUVEC) and mesenchymal stem cells (MSCs). The cell lines were from the PDMR collection (https://pdmr.cancer.gov/models/database.htm). The Ras mutation status of each line was known and included KRAS G12C (5 lines), KRAS G12D (7 lines) and 8 lines which were RAS WT or harbored other RAS mutations. The high concentration range of each agent was near the clinical Cmax for the drug or was 10 μM and decreased in half-logs covering a 3-log range. The complex spheroids were established for 3 days before drug(s) were added. Seven days after drug exposure the experiment was terminated with CellTiter-Glo 3D. Cell viability was determined relative to a vehicle treated control and IC50 values were calculated from concentration response curves. The concentration response for sotorasib and MRTX-1257 (except for concentrations 3 μM or higher) inhibitors were shallow to flat. The 4 KRAS G12C mutant line were most sensitive to sotorasib and MRTX-1257 with the KRAS G12C mutant pancreatic cancer line being the next most sensitive. A similar pattern was observed with the SHP-2 inhibitor TNO-155; however, there was no selectivity for the KRAS G12C mutant lines in response to the SOS-1 inhibitor BI-3406. There was heterogeneity in the occurrence of additivity/synergy amongst the KRAS G12C mutant lines in the combination studies likely indicating that factors in addition to KRAS mutations influenced response. The most successful combination was TNO-155 plus ipatasertib which resulted in more than 1-log of cell kill in 9 of 20 lines including the 5 KRAS G12C mutant lines. Venetoclax in combination with the RAS pathway inhibitors was active in 4 of the 5 KRAS G12C lines as was the combination of sapanisertib and TNO-155. TNO-155 in combination with sotorasib, MRTX-1257 or BI-3406 was highly active in the LG0567-F671 NSCLC harboring KRAS G12C. The response of the RAS mutant lines to the combinations was compared with the response of RAS WT lines to the same combinations. Often at the higher concentrations of the drugs/investigational agents, lines without RAS mutations are responsive. Next steps include PDX studies with the same tumor models. This project was funded in part with federal funds from the NCI, NIH, under contract no. HHSN261200800001E. Citation Format: Naoko Takebe, Thomas Dexheimer, Thomas Silvers, Rene Delosh, Julie Laudeman, Siddhartha Paul, Russell Reinhardt, Chad Ogle, Joel Morris, Nathan Coussens, James H. Doroshow, Beverly A. Teicher. RAS-pathway inhibitors (Sotorasib, MRTX-1257, TNO-155, BI-3406) in a Complex Spheroid Combination Screen with PDMR Cell Lines [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 1054.
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Kovač, Marta. "Prirodni inokulum entomopatogenih gljiva u prezimljavajućoj populaciji hrastove mrežaste stjenice Corythucha arcuata (Heteroptera, Tingidae)." Entomologia Croatica 20, no. 1 (December 29, 2021): 13–20. http://dx.doi.org/10.17971/ec.20.1.3.
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Prirodni neprijatelji invazivne hrastove mrežaste stjenice, Corythucha arcuata (Heteroptera, Tingidae) do sada su slabo istraživani, no otkriće entomopatogenih gljiva kao uzročnika uginuća odraslih jedinki nađenih na području spačvanskog bazena produbilo je potrebu za provedbom entomopatoloških istraživanja. Cilj ovog istraživanja bio je utvrditi brojnost hrastove mrežaste stjenice, prirodni mortalitet i prirodni inokulum entomopatogenih gljiva, odnosno njihovu prisutnost u populaciji računajući prosječan broj prezimljavajućih, mrtvih i zaraženih jedinki u 1 m2 mahovine na odabranim lokalitetima spačvanskog bazena. Istraživanje je provedeno u ožujku 2021. godine, a podaci su uspoređivani s onima iz ožujka 2019. godine. Rezultati su pokazali da na tim lokalitetima postoji određeni pad gustoće populacije ovog štetnika, a prezimljavajuću generaciju bilježi vrlo visoki mortalitet od 64%. Također, utvrđeno je povećanje prirodnog inokuluma entomopatogenih gljiva s 13% na 19% populacije, a u ukupnom udjelu zaraženih jedinki njih čak 75% bilo je zaraženo gljivama roda Beauveria. Navedeno povećanje prirodnog inokuluma ukazuje na redukcijski potencijal entomopatogenih gljiva i ulogu koji ti organizmi imaju u prirodnoj regulaciji populacija hrastove mrežaste stjenice, a koja bi s vremenom mogla biti sve važnija.
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Vranes, Branko. "Laza Kostic’s Santa Maria della Salute in the poetic scope of Branko Miljkovic and Borislav Radovic." Prilozi za knjizevnost, jezik, istoriju i folklor, no. 88 (2022): 51–63. http://dx.doi.org/10.2298/pkjif2288051v.
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The poetry of Laza Kostic left a strong impression on several Serbian interwar modernists, but it was in the years after the Second World War that Kostic?s role in the forming of modern poetic expression became completely clear to poets and commentators. Various aspects of the reception of Kostic?s poetry, especially of his poem Santa Maria della Salute, in Serbian interwar and post-war literary-critical thought have been largely explored. In this paper, I focus on the way in which members of the second generation of post-war modernists reacted to Kostic?s late poem in their verses. In the poem Laza Kostic [Laza Kostic] from the cycle Seven Dead Poets [Sedam mrtvih pesnika], which was written in 1956, Branko Miljkovic emphasized the autopoetic dimension of Santa Maria della Salute. In the poem Before the Pharmacy of Our Lady of the Sea [Pred lekarnicom u Gospe od mora], which was published in Poems 1971-1982 [Pesme 1971-1982], Borislav Radovic elaborated on ecological problems raised in Kostic?s late poem.
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Vysikaylo, Philip I., and Viktor V. Belyaev. "DEVELOPMENT OF ANATOLY A. VLASOV’S IDEAS AT MRTTI (MRSU): ON THE 110TH BIRTHDAY OF ANATOLY ALEXANDROVICH VLASOV." Bulletin of the Moscow State Regional University (Physics and Mathematics), no. 3 (2018): 107–16. http://dx.doi.org/10.18384/2310-7251-2018-3-107-116.
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Siddiqui, M., S. Balyansikova, I. Nagtegaal, N. West, G. Murray, S. Bosch, A. van-Tilberg, et al. "Agreement between histopathologic regression and MRI Tumour Regression (mrTRG) scales used to assess response for rectal cancers." International Journal of Surgery 55 (July 2018): S43—S44. http://dx.doi.org/10.1016/j.ijsu.2018.05.190.
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Woolstrum, J. M., C. E. Seyler, and R. D. McBride. "Hall instability driven seeding of helical magneto-Rayleigh–Taylor instabilities in axially premagnetized thin-foil liner Z-pinch implosions." Physics of Plasmas 29, no. 12 (December 2022): 122701. http://dx.doi.org/10.1063/5.0103651.
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Helical magneto-Rayleigh–Taylor instability (MRTI) structures have been observed in z-pinch-driven liner implosion experiments with a pre-imposed axial magnetic field. We show that the formation of these helical structures can be described by a Hall magnetohydrodynamical (HMHD) model. We used the 3D extended magnetohydrodynamics simulation code PERSEUS (which includes Hall physics) [Seyler and Martin, Phys. Plasmas 18, 012703 (2011)] to study these helical instabilities and show that a Hall interchange instability in low-density coronal plasma immediately surrounding the dense liner is responsible for producing helically oriented effects in the magnetic field and current density within the coronal layer. This seeds the helical pitch angle of the MRTI even when other proposed helical seeding mechanisms are either not present in the experiments or not accounted for in the simulations. For example, this mechanism does not require low-density power-feed plasmas to be swept in from large radius or the development of electrothermal instabilities. The Hall Instability is, thus, a new, independent explanation for the origin of the helical instabilities observed in axially premagnetized liner experiments. Simulation results supporting this mechanism are presented.
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Mladenovic, Ivica. "NOVE KLASNE BORBE U FRANCUSKOJ: SLUČAJ „ŽUTIH PRSLUKA”." Srpska politička misao 68, no. 2/2020 (August 3, 2020): 275–97. http://dx.doi.org/10.22182/spm.6822020.12.
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Jedanaestoro mrtvih, preko četiri hiljade lakše i više od stotinu teško povređenih (od čega sedamnaest izbijenog oka i četiri pokidane ruke): ovo je dosadašnji crni bilans represije nad „Žutim prslucima”. Francuska ne pamti ovako dramatičnu društveno-političku situaciju – izazvanu unutrašnjim rascepima – još od ubistva alžirskih studenata 1961. Čak je i Evropski parlament, 14. februara 2019. godine, osudio nesrazmernu upotrebu sile i korišćenje, u ostatku Evrope inače zabranjenih, GLI-F4 granata za razbijanje demonstracija, dok je UN, kada je reč o policijskom nasilju, Francusku svrstao u isti rang sa Sudanom i Zimbabveom. O situaciji u zemlji najslikovitije možda govori izvođenje vojske na ulice tokom tzv. akta XIX „Žutih prsluka”, u subotu 23. marta 2019. godine. Ovaj rad nudi odgovore na nekoliko pitanja: Da li je ovaj specifičan pokret moguće tumačiti u svetlu novih klasnih rascepa u Francuskoj, i na koji se on način uklapa u Marksov koncept klasne borbe? Koje su socijalne grupe koje čine njegovo jezgro i po čemu se one razlikuju od tradicionalnih klasa? U kojim socijalnim grupama nalazimo najveću podršku metodama borbe ovog pokreta? Koje su njegova ključna svojstva i po čemu se razlikuje u odnosu na prethodne socijalne pokrete u Francuskoj, kao i, generalno, savremene društvene pokrete u Evropi? Kakva je politička artikulacija i politički potencijal „Žutih prsluka”?
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Olivos O., Luis, José Juárez E., and Julio De Lucas B. "Estudio farmacocinético comparativo entre diazepam y midazolam, post administración intravenosa e intramuscular en avestruz doméstico (Struthio camelus)." Ciencia e Investigación 4, no. 1 (June 18, 2001): 40–49. http://dx.doi.org/10.15381/ci.v4i1.3392.
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El interés en el manejo de especies animales con fines experimentales y de estudios farmacocinéticos, por la necesidad de pautas terapéuticas más racionales, nos llevó a desarrollar el presente estudio. Se utilizaron seis avestruces juveniles sanos, a los que se administró diazepam (DZP) y midazolam (MDZ) vía IV e IM, a dosis única de O,5mg/kpv. En cuanto a DZP, la cuantificación por HPLC/UV(220nm) de los parámetros farmacocinéticos post administración IV, nos dieron los siguientes valores: MRTi=71,17±29,97min, t1/2β=49,30±21,18min, Cli=87±50mUkglmin y Vc=1,82±O,35L/kpv; mientras que las concentraciones plasmáticas post administración IM, no permitieron el tratamiento cinético. Para MDZ los parámetros farmacocinéticos post administración IV e IM corresponden respectivamente a MRTi=59,70±37,14 y 50,09±12,71min, 1/2β=39,64±25,30 y 35,32±1O,93min, Cli=37±20mL/kg/min y Vc=O,72±O,24L/kpv. La biodisponibilidad post administración IM fue 99,97±30,43% con Cmax=O,36±O,09 μ,g/mL y tmax=1l±2,24min. No se detectaron concentraciones plasmáticas de l-hidroxi-midazolam (metabolito activo). Existe una correlación entre los datos de laboratorio obtenidos y las observaciones clínicas in situ. Se concluye que DZP no fue una opción válida para tranquilización o inducción anestésica en avestruces juveniles. MDZ sí demostró una depresión del SNC significativa por ambas vías. La técnica analítica utilizada es confiable de acuerdo a los parámetros de validación empleados.
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Arocena, Gastón M., Rodrigo Sieira, Diego J. Comerci, and Rodolfo A. Ugalde. "Identification of the Quorum-Sensing Target DNA Sequence and N-Acyl Homoserine Lactone Responsiveness of the Brucella abortus virB promoter." Journal of Bacteriology 192, no. 13 (April 16, 2010): 3434–40. http://dx.doi.org/10.1128/jb.00232-10.
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ABSTRACT VjbR is a LuxR-type quorum-sensing (QS) regulator that plays an essential role in the virulence of the intracellular facultative pathogen Brucella, the causative agent of brucellosis. It was previously described that VjbR regulates a diverse group of genes, including the virB operon. The latter codes for a type IV secretion system (T4SS) that is central for the pathogenesis of Brucella. Although the regulatory role of VjbR on the virB promoter (P virB ) was extensively studied by different groups, the VjbR-binding site had not been identified so far. Here, we identified the target DNA sequence of VjbR in P virB by DNase I footprinting analyses. Surprisingly, we observed that VjbR specifically recognizes a sequence that is identical to a half-binding site of the QS-related regulator MrtR of Mesorhizobium tianshanense. As shown by DNase I footprinting and electrophoretic mobility shift assays, generation of a palindromic MrtR-like-binding site in P virB increased both the affinity and the stability of the VjbR-DNA complex, which confirmed that the QS regulator of Brucella is highly related to that of M. tianshanense. The addition of N-dodecanoyl homoserine lactone dissociated VjbR from the promoter, which confirmed previous reports that indicated a negative effect of this signal on the VjbR-mediated activation of P virB . Our results provide new molecular evidence for the structure of the virB promoter and reveal unusual features of the QS target DNA sequence of the main regulator of virulence in Brucella.
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Siddiqui, M. R. S., K. L. Gormly, J. Bhoday, S. Balyansikova, N. J. Battersby, M. Chand, S. Rao, P. Tekkis, A. M. Abulafi, and G. Brown. "Interobserver agreement of radiologists assessing the response of rectal cancers to preoperative chemoradiation using the MRI tumour regression grading (mrTRG)." Clinical Radiology 71, no. 9 (September 2016): 854–62. http://dx.doi.org/10.1016/j.crad.2016.05.005.
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